CN103394053B - Traditional Chinese medicine composition capable of preventing and treating piglet yellow and white scours as well as preparation and applications thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine composition for preventing and treating yellow scour of piglets, which is prepared from the following components in parts by weight: Huoxiang 200±20 parts; Codonopsis 180±10 parts; Atractylodes macrocephala 100±10 parts; 10 parts; Pinellia 100±10 parts; Poria 100±10 parts; ebony 100±10 parts; ginger 60±5 parts; roasted licorice 60±5 parts. The traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum of the invention has the advantages of high cure rate, less toxic and side effects, and less drug resistance.

Description

A traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum and its preparation and application

technical field

The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum and its preparation and application.

Background technique

Piglet yellow and pullorum are infectious diseases caused by enterotoxigenic Escherichia coli (ETEC). They are the most common clinical infectious diseases of piglets before 30 days of age. Their high morbidity and mortality rates seriously affect the development of pig industry. Often bring great harm and economic loss to the pig industry. Among them, yellow scour of piglets often occurs within 1 week after piglets are born, mostly at the age of 1 to 3 days, and often occurs in the whole litter, with an incidence rate of more than 90% and a mortality rate of up to 100%. Piglet pullorum mostly occurs in 10-30 day-old piglets, most of whom are 10-20 days old, and the incidence rate can reach 30%-80%.

Yellow scour of newborn pig-lets , also known as colibacillosis of newborn piglets or early-onset colibacillosis of piglets, is an acute and highly fatal intestinal infectious disease of newborn piglets within one week of age. The main features are diarrhea, excretion of yellow loose feces, and acute death. Watery feces, yellow or grayish yellow, containing small flakes of curd and small air bubbles, sick pigs are thirsty, reduce milk intake, dehydration, emaciation, coma, and exhaustion. Common pathological changes of enteritis and sepsis, high morbidity and mortality.

White scour of piglets, also known as delayed colibacillosis, is an acute infectious disease that often occurs in piglets aged 10 to 30 days. It is mainly characterized by excreting milky white or off-white loose feces with a fishy smell. The piglets suddenly have loose stools, and the same litters occur one after another, with white, off-white, fishy, mushy or pulpy feces. Piglets lack energy, fear of cold, dehydration, reduced or no milk intake, and sometimes spit up milk. Generally, the condition of sick pigs is relatively mild, and can be cured by timely treatment, but most of them become stiff pigs due to repeated attacks. In severe cases, pigs suffer from fecal incontinence and die in about 1 week.

Piglet yellow and pullorum mostly occur in piglets aged 10 to 30 days, within 7 days of age, and piglets over 30 days of age rarely develop the disease. There is no seasonality, and the climate changes drastically in winter and spring, and it is more common when it is rainy, humid or poorly kept warm, and the sows lack milk. The disease seriously affects the growth and development of piglets. Therefore, in production practice, the prevention and control of piglet yellow and pullorum should be regarded as the top priority of pig farm disease control. Piglet yellow and pullorum are currently mainly treated with antibiotics clinically. Although antibiotics can inhibit or kill many pathogenic E. coli, there are a large number of non-pathogenic bacteria in the intestinal tract of the body in addition to pathogenic E. It plays a very important role in the normal metabolism of the body. Blind use of antibiotics will also inhibit or kill many non-pathogenic bacteria and destroy their composition, seriously affecting the digestive function of the body and aggravating the disease; develop drug resistance. The wanton abuse of chemical drugs, antibiotics and monomeric drugs not only produces many drug-induced diseases such as multidrug resistance and receptor hypersensitivity, but also causes pollution of livestock and poultry products, endangering human food hygiene and safety. Traditional Chinese medicine is derived from animals, plants and minerals, and contains a variety of biologically active ingredients. Most of them are made of natural plants. They are rich in resources, easy to obtain, good in effect, low in price, have minimal toxic and side effects, almost no residue, and no antibiotics. Medicinal properties, do not pollute the environment. Therefore, it is particularly important to select traditional Chinese medicine preparations to replace chemical medicines and antibiotics.

Contents of the invention

The technical problem to be solved by the invention is to overcome the existing defects and provide a traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum with high cure rate, low toxicity and side effects, and less resistance.

In order to solve the problems of the technologies described above, the present invention provides the following technical solutions:

A traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum, prepared from the following components in parts by weight:

Huoxiang 200±20 parts; Codonopsis 180±10 parts; Atractylodes macrocephala 100±10 parts; Purslane 100±10 parts; Pinellia 100±10 parts; parts; licorice 60 ± 5 parts.

Preferably, it is prepared from the following components by weight:

Huoxiang 200 parts; Codonopsis 180 parts; Atractylodes macrocephala 100 parts; Purslane 100 parts; Pinellia 100 parts;

A preparation method of a traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum, comprising the following steps:

Take each component in parts by weight, add 5 times the weight of the medicinal materials in water, decoct twice, 2 hours each time, combine the decoction, filter, and let the filtrate stand for 12 hours, take the supernatant and concentrate to a relative density of 1.20 ～1.30, add ethanol until the alcohol content is 60%, let it stand, filter, recover the ethanol, add water until the relative density is 1.10～1.20, and obtain.

Further, 1.5 parts of potassium sorbate was added, mixed evenly, cooled for 48 hours, filtered and divided into brown sterile polyester bottles, and steamed at 100°C for 30 minutes to obtain oral liquid.

Application of a traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum in the preparation of medicines for preventing and treating piglet yellow and pullorum.

The invention also provides a traditional Chinese medicine compound oral liquid for preventing and treating piglet yellow and pullorum, which contains the traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum and pharmaceutically acceptable auxiliary materials.

In the Chinese medicine compound oral liquid for preventing and treating piglet yellow and pullorum above, the Chinese medicine composition for preventing and treating piglet yellow and pullorum is:

Huoxiang 200±20 parts; Codonopsis 180±10 parts; Atractylodes macrocephala 100±10 parts; Purslane 100±10 parts; Pinellia 100±10 parts; parts; licorice 60±5 parts,

or

Huoxiang 200 parts; Codonopsis 180 parts; Atractylodes macrocephala 100 parts; Purslane 100 parts; Pinellia 100 parts; The oral liquid is specifically prepared through the following steps:

Take each component in parts by weight, add 5 times the weight of the medicinal materials in water, decoct twice, 2 hours each time, combine the decoction, filter, and let the filtrate stand for 12 hours, take the supernatant and concentrate to a relative density of 1.20 ～1.30, add ethanol to 60% alcohol content, place, filter, recover ethanol, add water until the relative density is 1.10～1.20, add 1.5 parts of potassium sorbate, mix well, cool for 48h, filter with brown sterile polyester Pack in separate bottles, and steam at 100°C for 30 minutes to obtain the product.

The present invention has the following beneficial effects:

The medicament of the present invention can be directly fed to livestock and poultry when it is specifically used.

Chinese veterinarians believe that the internal cause of piglet yellow and pullorum is the weak spleen and stomach of piglets. Piglets grow vigorously and rapidly, and require more water and grain essence than adult pigs, and the burden on the spleen and stomach is heavy. In addition, piglets are easily injured by diet, so they are prone to diarrhea. The external causes are mainly (1) Feeling the evils of the season: feeling cold, damp, filthy, turbid and pestilential qi, or sleeping in the open for cold and damp, invasion of cold and dampness, guest evil and foul qi, depression and suffocation of the middle burner, all of which can cause spleen and stomach injuries, abnormal transportation and transformation, and qi Unfavorable movement, loss of ascending and descending, clear and turbidity related, disorder in the stomach, and diarrhea. (2) Unclean feeding: Unclean feeding, rotten and spoiled food by mistake, or cold and cold feed can damage the spleen and stomach most, and turbidity and turbidity are mixed together to form diarrhea.

To sum up, the disease is mainly due to the delicate viscera and viscera of piglets, lack of physical qi or congenital dysplasia and acquired nutritional deficiencies, which lead to weak spleen and stomach and decreased resistance of piglets. Spleen and stomach, dampness and poison trap the spleen, deficiency of spleen and stomach qi, and stagnation of yang in the middle, resulting in stagnation of qi movement, ups and downs and disorder, the spleen governs movement and transformation and raises clear yang, stomach governs acceptance and lowers turbid yin, now there is cold in the middle of the deficiency, and the rise and fall Dereliction of duty, so it is abdominal pain, not wanting to eat or drink; the digestion and absorption function of the spleen and stomach is disturbed, clear and turbid are mixed and descended, damp and hot are pressed down, the large and small intestines are not secreted, and water and dampness cannot be divided, resulting in diarrhea, so feces Thin, fishy, or stagnant in the intestines, causing intestinal qi and blood to block and turn into white pus, so the diarrhea is white or white with pus.

It is suitable for invigorating the spleen and dispelling dampness, harmonizing the stomach and relieving diarrhea, astringing and astringent, clearing away heat and detoxification.

This prescription is concentrated from traditional Chinese medicines such as Huoxiang, Codonopsis, Atractylodes Rhizoma Atractylodes Rhizome, Black Plum, etc. It can invigorate the spleen and dispel dampness, harmonize the stomach and detoxify the root cause, astringe the astringent and treat the symptoms to achieve the purpose of treating yellow and white scour of piglets.

The medicine of the present invention is based on using Huoxiang pungent and warm to dispel cold, aromatize dampness, regulate qi and neutralize, harmonize the stomach and delight the spleen, and treat both the exterior and interior as king. Ginger warms the yang of the middle burner and releases the cold in the interior, which can relieve nausea and relieve stomach qi, and make the stomach qi smooth. Pinellia dries dampness and lowers adverse events, harmonizes the stomach and relieves nausea; Atractylodes macrocephala invigorates the spleen and eliminates dampness, helps Codonopsis ginseng to invigorate qi; Poria cocos invigorates the spleen and transports dampness; purslane has the effects of clearing away heat and detoxification, dispersing blood and reducing swelling, and can cure dysentery, pus and blood, Black plum tastes sour and astringent, flat in nature, astringent in the intestines, relieves diarrhea, promotes body fluid, and is an adjuvant altogether. Glycyrrhiza invigorates qi, harmonizes urgency, relieves the poison of various medicines, and relieves the pungent nature of ginger. When all medicines are used together, both symptoms and root causes can be treated.

The medicine of the present invention has the functions of replenishing qi, invigorating the spleen and removing dampness, strengthening the body and dispelling pathogenic factors, harmonizing the stomach and relieving diarrhea, astringent and astringent, detoxifying and turbidity expelling, dispelling cold and relieving pain. The formula fully refers to the eight principles of veterinary medicine, Wei Qi Ying Xue, and the theory of visceral syndrome differentiation, taking into account both inside and outside, treating Qi and blood at the same time, specializing in medicine, and rigorous compatibility. The way to use soldiers lies in precision, and the way to use medicine lies in the pure composition characteristics.

Modern veterinary pharmacological research and long-term clinical practice have proved that traditional Chinese medicine has the advantages of high overall curative effect, low drug resistance, low residue, and small toxic and side effects. It has a significant inhibitory effect on the growth and reproduction of many viral pathogens, even reaching The pharmacological effect of killing pathogens. According to the etiology, pathogenesis, clinical pathology and drug action mechanism of piglet yellow and pullorum, the present invention carries out the prevention and treatment of piglet yellow and pullorum with Chinese medicine compound, and develops a Chinese medicine compound preparation with remarkable curative effect, reliable quality, convenient application and low price, and The popularization and application in pig production has very important economic and social significance for promoting the healthy development of pig industry.

The medicine of the present invention can be directly fed to pigs when it is specifically used.

The usage amount of medicine of the present invention is: therapeutic dose: piglet, 2ml/kg. BW, 1 time/d, continuous use for 3-5 days; preventive dose: piglets, 1ml/kg. BW, 1 time/d, for 3-5 consecutive days.

Detailed ways

Product and preparation example

Example 1

A traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum, prepared from the following components in parts by weight:

Huoxiang 220 parts; Codonopsis 190 parts; Atractylodes macrocephala 110 parts; Purslane 110 parts; Pinellia 110 parts;

A preparation method of a traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum, comprising the following steps:

Take each component in parts by weight, add 5 times the weight of the medicinal materials in water, decoct twice, 2 hours each time, combine the decoction, filter, and let the filtrate stand for 12 hours, take the supernatant and concentrate to a relative density of 1.20 ～1.30, add ethanol until the alcohol content is 60%, let it stand, filter, recover the ethanol, add water until the relative density is 1.10～1.20, and obtain.

Further, add 1.5 parts of potassium sorbate, mix well, cool for 48 hours, filter and pack in brown sterile polyester bottles, and circulate steam at 100°C for 30 minutes to obtain oral liquid.

Example 2

A traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum, prepared from the following components in parts by weight:

Huoxiang 180 parts; Codonopsis 170 parts; Atractylodes macrocephala 90 parts; Purslane 90 parts; Pinellia 90 parts;

Its preparation method is identical with embodiment 1.

Example 3

The traditional Chinese medicine composition for preventing and treating piglet yellow and pullorum is prepared from the following components:

Huoxiang 200g, Codonopsis 180g, Atractylodes macrocephala 100g, purslane 100g, Pinellia 100g, Poria cocos 100g, ebony plum 100g, ginger 60g, licorice 60g.

Its preparation method is carried out according to the following steps: add the above 9 flavors according to the parts by weight, add 5 times the weight of medicinal materials in water, decoct twice for 2 hours each time, combine the decoctions, filter, and let the filtrate stand for 12 hours, take the above Concentrate the supernatant to a relative density of 1.20-1.30, add ethanol to an alcohol content of 60%, let it stand, filter, recover the ethanol, add water to a relative density of 1.10-1.20, add 1.5 g of potassium sorbate, mix well, and cool for 48 hours. Filter and pack in brown sterile polyester bottles, steam at 100°C for 30 minutes, and store for later use.

Application example

1) Treatment trials:

In this example, a randomized double-blind test method was used to conduct a treatment test of different doses for the treatment of naturally occurring yellow scours and pullorum in piglets, and enrofloxacin soluble powder was selected as the positive drug control group. Observe the therapeutic effect of the present invention on piglet yellow and pullorum, so as to explore the feasibility of its application in livestock and poultry production.

Treatment test for naturally occurring yellow and pullorum of piglets: the sick pigs with natural onset of yellow and pullorum were randomly divided into 5 groups with 36 piglets in each group. The 1st-3 group is to adopt the drug treatment group of the present invention (establishing three groups of low, middle and high doses respectively); The 4th group is enrofloxacin soluble powder positive drug control group; The 5th group is positive control group (infection , without administration). They were kept in isolation, and the symptoms, cure, and death of sick pigs were recorded in detail.

Administration method, medication time: Medicine 1 group: every pig is fed with 5ml of the medicine of the present invention every day, and fed continuously for 3 days; Medicine 2 groups: every pig is fed with 10ml of medicine of the present invention every day, continuously fed for 3 days ; Medicine 3 groups: every pig was fed with 15ml of the medicine of the present invention every day for 3 days. Positive drug control group: each pig was fed with enrofloxacin soluble powder (used according to the instructions) every day for 3 consecutive days; positive control group: no drug administration.

Experimental results:

After 24 hours of drug feeding in the treatment group, the spirit of the diseased pigs in the drug group was better than that of the positive control group. After 36 hours, the number of deaths was counted, and the number of deaths of the diseased pigs in the drug group was significantly less than that of the positive control group; However, the positive control group still died one after another. After 3 days, the spirit and appetite of the sick pigs in the drug group were greatly improved (as seen from the increase in the amount of milk and feed), and the feces were normal.

The statistical results of the test show that among the 36 piglets in the high-dose treatment group of the present invention, 33 recovered from illness, and 3 still had diarrhea, but did not die, and the cure rate was 91.6%; among the 36 piglets in the middle-dose treatment group, 32 recovered from illness, 4 piglets were still diarrhea, but did not die, the cure rate was 88.9%; among the 36 piglets in the low-dose treatment group, 26 piglets were cured, 8 piglets were still diarrhea, and 2 piglets died, the cure rate was 72.2%; enrofloxacin soluble powder Of the 36 piglets in the treatment group (control group), 26 were cured, 5 were still loose, and 5 died, with a cure rate of 72.2%; among the 36 piglets in the positive control group (no drug administration), 24 The head is still loose, and 12 are dead, and the death rate of the high and middle dose treatment groups is 0.0%, and the sick pigs of the treatment group are compared with the sick pigs of the control group, showing that the symptoms are alleviated, and the course of the disease is shortened. Doses have therapeutic effects on piglet yellow and pullorum, can relieve symptoms and reduce mortality, and high and medium doses are superior to enrofloxacin soluble powder in reducing mortality and improving cure rate. Both high and medium doses of the present invention can achieve the effect of curing diseases and improving the survival rate, and there is no difference between the two at the level of 0.05, so the medium dose can be used in clinical use, which is economical and effective.

) prevention test

In the pig farm where the epidemic occurred, 100 healthy pigs without disease were randomly divided into 2 groups, 50 in each group, the first group was the drug prevention group; the second group was the control group; each pig in the first group was fed daily Medicine 10ml of the present invention (continuously feed 7 days; The 2nd group does not add; Other measures of two groups of pigs are all by the normal disinfection immunization procedure of pig farm.

The results showed that the incidence rate of yellow and pullorum in the drug prevention group was 3.0%, and the incidence rate in the control group was 75.0%. The spirit and appetite of the diseased pigs in the drug group were better than those in the control group, and the disease symptoms were lighter than those in the control group. After the prevention group was given treatment after the onset, the cure rate was 86.0%, while that of the control group was 67.0%.

From the test results, the present invention has the functions of replenishing qi, invigorating the spleen and removing dampness, strengthening the body and dispelling pathogenic factors, harmonizing the stomach and relieving diarrhea, astringent and astringent, detoxification and turbidity removal, dispelling cold and relieving pain. No adverse reactions were found in the drug during the clinical trials. High, medium and low doses have good therapeutic effect on piglet yellow and pullorum. The therapeutic effect of the low-dose group was lower than that of the high-dose and middle-dose groups, and there was no significant difference in mortality, cure rate and total effective rate between the middle-dose and high-dose groups. According to the severity of the disease and the cost of treatment, it is recommended to use medium and high doses (10-15ml for piglets) for clinical application; preferably, it should be used continuously for 3-5 days.

In order to clarify the toxic and side effects of the present invention, in order to make an accurate and objective evaluation of its safety, provide a scientific basis for clinical trial medication, and ensure the safety of clinical medication, the toxicity test of the present invention was carried out.

The LD ₅₀ and maximum tolerance dose test of the present invention on mice showed that after oral administration according to the designed dose, the behavioral signs of the mice in each group were normal within 7 days, and no symptoms of poisoning and death occurred. Therefore, the LD ₅₀ cannot be measured, and the maximum dose of 50 g/kg has to be taken as the highest dose. After culling on the 8th day, there were no pathological changes in the organs of the mice in each group, and no pathological features were found in the histological examination of the liver, kidney, and spleen. Hemogram and serum GPT activity detection showed no significant difference with normal mice.

In order to grasp the toxic and side effects and the severity of the continuous use of the Chinese herbal medicine compound of the present invention, as well as the development and recovery after drug withdrawal, provide reference for formulating safe clinical dosage. The subacute toxicity test of the present invention was carried out on mice. During the test period, except that the high-dose group caused poor palatability due to the large dose of the drug and affected food intake, the diet, behavior, and feces of the mice in all other groups were normal and well-developed. , without any symptoms of poisoning, except for the significant difference between the high-dose group and the control group in weight gain and nutrition, the rest had no significant difference. The hemogram and serum GPT of each group were within the normal range. Histological examination showed no obvious poisoning lesions in liver, kidney, spleen, etc. According to 3-10 times of the clinical dosage, it is fed for 30 days, and no toxic and side effects are seen, which shows that the clinical dosage of the preparation is very safe.

The drug of the present invention is subjected to an accelerated test for 3 months under the condition of temperature: 40°C±2°C and relative humidity: 75%±5% according to the packaging conditions on the market, and the quality has no obvious change. In the long-term test for 24 months under the condition of temperature: 25℃±2℃ and relative humidity: 60%±10%, the quality has no obvious change. The above test results show that the medicine of the present invention is stable in storage quality under specified conditions.

Finally, it should be noted that: the above is only a preferred embodiment of the present invention, and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, it still The technical solutions recorded in the foregoing embodiments may be modified, or some technical features thereof may be equivalently replaced. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (5)

1. prevent and treat a Chinese medicine composition for piglet Huang, Hakuri, it is characterized in that, prepared by the component of following weight portion: Herba Pogostemonis 200 ± 20 parts; Radix Codonopsis 180 ± 10 parts; The Rhizoma Atractylodis Macrocephalae 100 ± 10 parts; Herba Portulacae 100 ± 10 parts; The Rhizoma Pinelliae 100 ± 10 parts; 100 ± 10 parts, Poria; Fructus Mume 100 ± 10 parts; 60 ± 5 parts, Rhizoma Zingiberis Recens; Radix Glycyrrhizae Preparata 60 ± 5 parts.

2., according to the Chinese medicine composition of the control piglet Huang described in claim 1, Hakuri, it is characterized in that, prepared by the component of following weight portion: Herba Pogostemonis 200 parts; Radix Codonopsis 180 parts; The Rhizoma Atractylodis Macrocephalae 100 parts; Herba Portulacae 100 parts; The Rhizoma Pinelliae 100 parts; 100 parts, Poria; Fructus Mume 100 parts; 60 parts, Rhizoma Zingiberis Recens; Radix Glycyrrhizae Preparata 60 parts.

3. the control piglet described in claim 1 or 2 is yellow, the preparation method of the Chinese medicine composition of Hakuri, comprises the following steps: get each component by weight, adds the water of medical material 5 times of weight, decoct 2 times, each 2 hours, collecting decoction, filter, filtrate leaves standstill 12 hours, and getting supernatant concentration to relative density is 1.20 ~ 1.30, add ethanol to alcohol content 60%, place, filter, reclaim ethanol, adding water to relative density is 1.10 ~ 1.20, to obtain final product.

4. the control piglet described in claim 1 or 2 is yellow, the application of the Chinese medicine composition of Hakuri in preparation control piglet Huang, Hakuri medicine.

5. a control piglet is yellow, the traditional Chinese medicine compound oral liquid of Hakuri, it is characterized in that, yellow containing the control piglet described in claim 1 or 2, the Chinese medicine composition of Hakuri and potassium sorbate, obtain especially by following steps: get each component by weight, add the water of medical material 5 times of weight, decoct 2 times, each 2 hours, collecting decoction, filter, filtrate leaves standstill 12 hours, getting supernatant concentration to relative density is 1.20 ~ 1.30, add ethanol to alcohol content 60%, place, filter, reclaim ethanol, adding water to relative density is 1.10 ~ 1.20, add potassium sorbate 1.5 parts, be mixed, cooling 48h, filter with brown aseptic polyester bottles subpackage, 100 DEG C of flowing steams 30 minutes, obtain.