CN103243046B - A kind of growth promoter and using method thereof impelling Photosynthetic bacterium strain Fast-propagation - Google Patents
A kind of growth promoter and using method thereof impelling Photosynthetic bacterium strain Fast-propagation Download PDFInfo
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- CN103243046B CN103243046B CN201310158292.7A CN201310158292A CN103243046B CN 103243046 B CN103243046 B CN 103243046B CN 201310158292 A CN201310158292 A CN 201310158292A CN 103243046 B CN103243046 B CN 103243046B
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- 241000894006 Bacteria Species 0.000 title claims abstract description 107
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 100
- 239000007952 growth promoter Substances 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 35
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 37
- 230000001580 bacterial effect Effects 0.000 claims abstract description 21
- 230000008569 process Effects 0.000 claims abstract description 15
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000004334 sorbic acid Substances 0.000 claims abstract description 13
- 235000010199 sorbic acid Nutrition 0.000 claims abstract description 13
- 229940075582 sorbic acid Drugs 0.000 claims abstract description 13
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 11
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 11
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 11
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 11
- 238000005286 illumination Methods 0.000 claims abstract description 9
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- 239000002253 acid Substances 0.000 claims abstract description 8
- 230000004913 activation Effects 0.000 claims abstract description 7
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- 238000004090 dissolution Methods 0.000 claims abstract description 6
- LROWVYNUWKVTCU-STWYSWDKSA-M sodium sorbate Chemical compound [Na+].C\C=C\C=C\C([O-])=O LROWVYNUWKVTCU-STWYSWDKSA-M 0.000 claims abstract description 6
- 235000019250 sodium sorbate Nutrition 0.000 claims abstract description 6
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- 238000000855 fermentation Methods 0.000 claims description 21
- 230000004151 fermentation Effects 0.000 claims description 21
- 230000012010 growth Effects 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 150000003839 salts Chemical class 0.000 claims description 10
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 6
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- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
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- 239000000047 product Substances 0.000 claims description 6
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- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 3
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
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- 229930003756 Vitamin B7 Natural products 0.000 claims description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- 235000019270 ammonium chloride Nutrition 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 235000002867 manganese chloride Nutrition 0.000 claims description 3
- 239000011565 manganese chloride Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- 235000011912 vitamin B7 Nutrition 0.000 claims description 3
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- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
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- 230000009471 action Effects 0.000 description 3
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
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- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of growth promoter and the using method thereof of impelling Photosynthetic bacterium strain Fast-propagation, its growth promoter is by weight by the one in 1 part of Sorbic Acid, potassium sorbate or sodium sorbate or mixture, join in 1-4 part ethanol, stirring, mixed dissolution process, obtain this product growth promoter; Growth promoter is joined in the photosynthetic bacterium liquid substratum prepared, be stirred to abundant dissolving, the photosynthetic bacterium bacterial classification of access activation, load in airtight or semi-open light transmission container, at 25 ~ 32 DEG C, 1500-6000lux illumination cultivation, the concentration adding the sorb acid group of growth promoter in described photosynthetic bacterium liquid substratum controls within the scope of 0.005 ~ 1.0mmol/L, advantage of the present invention is easy to use, and consumption is few, and cost is low, photosynthetic bacterial thallus Fast-propagation can be promoted, and use safety, to eco-friendly, there are wide market outlook.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of growth promoter and the using method thereof of impelling Photosynthetic bacterium strain Fast-propagation.
Background technology
Photosynthetic bacterium (PhotosyntheticBacteria is called for short PSB) ecologicaI distribution is very extensive, and being mainly distributed in ocean, rivers, lakes and marhshes, active sludge and soil, is that a class is aquatic, hydrosphere microorganism.Photosynthetic bacterium is using sunlight as the energy, with gas chromatographies such as sodium-acetate, Sodium.alpha.-hydroxypropionate, N.F,USP MANNITOL, oxysuccinic acid, citric acid, glucose, glycerine for Carbon and nitrogen sources, there is the features such as growth and breeding speed is fast, substratum wide material sources, meta-bolites are nutritious.Photosynthetic bacterial thallus contains rich in protein, amino acid, biological required VITAMIN, the antiviral activity factor, Coenzyme Q10 99.0 and several physiological active substances, can provide organism necessary nutrition, promote animal and plant growth, strengthen its disease-resistant function, reduce the generation of disease, improve biological survival rate.Its product can be widely used in all many-sides such as fodder industry, agriculture production, environment protection, is more and more subject to scientific worker and business people's attention.
The important index of photosynthetic bacterium product is somatic cells quantity and purity.Existing photosynthetic bacterium production technique mostly adopts open or semi open model liquid illumination cultivation, by improving medium nutrient content, increasing intensity of illumination, improve pH value, creating the culture environment of relative anaerobism and suppress varied bacteria growing.But too high pH value can suppress the growth of photosynthetic bacterium itself, make final somatic cells quantity on the low side; And be that raw material (substratum) is cultivated in the process of photosynthetic bacterium with industrial and agricultural wastewater, because water body is mostly in subacidity, not only cost is high to consume a large amount of alkali lye heightening pH value, and contaminate environment, in this micro-acid or neutral aerobic environment, the infection of miscellaneous bacteria also will be inevitable, be unfavorable for the foundation of photosynthetic bacterium dominant population.In a word, existing photosynthetic bacterium production technique, in strain growth speed, the somatic cells quantity contained, thalline purity, can not meet the requirement of production application far away.
Summary of the invention
The present invention, for solving the problems of the prior art, provides a kind of and can impel the quick growth and breeding of Photosynthetic bacterium strain, the growth promoter greatly improving somatic cells quantity and using method thereof.
The present invention is achieved by the following technical solutions:
A kind of growth promoter impelling Photosynthetic bacterium strain Fast-propagation, described growth promoter is: by weight by the one in 1 part of Sorbic Acid, potassium sorbate or sodium sorbate or mixture, join in 1-4 part ethanol, stirring, mixed dissolution process, obtain this product growth promoter;
The temperature of described stirring, mixed dissolution process is-10 ~ 45 DEG C, and the time is 1 ~ 10min;
Described ethanol is medical 75-95% ethanol.
A kind of using method impelling the growth promoter of Photosynthetic bacterium strain Fast-propagation:
Joined by growth promoter in the photosynthetic bacterium liquid substratum prepared, be stirred to abundant dissolving, the photosynthetic bacterium bacterial classification of the activation of access 10-30%, loads in airtight or semi-open light transmission container, at 25 ~ 32 DEG C, and illumination cultivation;
The concentration adding the sorb acid group of growth promoter in described photosynthetic bacterium liquid substratum controls within the scope of 0.005 ~ 1.0mmol/L.
Preferably, the concentration adding the sorb acid group of growth promoter in described photosynthetic bacterium liquid substratum is: 0.01 ~ 0.5mmol/L.Be more preferably: 0.03 ~ 0.1mmol/L.
Described photosynthetic bacterium liquid substratum refers to that the city organic industrial sewage of seed fermentation substratum or comprehensive treating process in the usual way, agricultural byproducts waste or industrial waste are commonly used in laboratory.
It is composed as follows that seed fermentation substratum is commonly used in described laboratory:
Ammonium chloride 1.0g, sodium-acetate 1.0g, magnesium sulfate 0.2g, sodium bicarbonate 1.0g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.2g, ironic citrate 0.005g, yeast extract paste 1.0g, peptone 1.0g, inorganic salts solution 10mL, growth cofactor 0.01g, distilled water 1000mL, PH=6.5 ~ 8.0;
Wherein grow cofactor to comprise: one or more materials in VITMAIN B1, amino-benzene methyl alcohol, vitamin H;
Wherein inorganic salts solution refers to: containing ironic citrate 5mg, calcium sulfate 0.05mg, boric acid 1mg, manganous chloride 0.05mg, zinc sulfate 1mg in often liter of distilled water.
The preparation method of the photosynthetic bacterium bacterial classification of described activation is:
Seed fermentation substratum is commonly used in the photosynthetic bacterium laboratory prepared, load in the transparent glass anaerobism bottle of 150ml capacity, through 115 DEG C of autoclavings 30 minutes, after cooling, strict aseptic technique, access Rhodopseudomonas palustris Rhodopeudomonaspalustris bacterial classification, culture presevation CGMCC1.2180,1500-6000lux illumination cultivation 3-5 days, culture temperature 28-32 DEG C, the photosynthetic bacterium bacterial classification of obtained activation.
Culture presevation number is the Rhodopseudomonas palustris Rhodopeudomonaspalustris bacterial classification of CGMCC1.2180 is the bacterial classification that national DSMZ buys.
The present invention compared with prior art has following significant advantage:
1. promote photosynthetic bacterium Fast-propagation.Under identical Adaptable growth condition, it is fast that application the present invention makes photosynthetic bacterium growth breed, and more do not add this photosynthetic bacterium promotor reproduction speed fast 1 ~ 2 day, greatly improves product cell concentration.
2. addition is few, use safety.This impels the growth promoter of Photosynthetic bacterium strain Fast-propagation to belong in food to allow the additive used, and addition is few, belongs to safe interpolation scope, can be used for all many-sides such as agricultural, environmental protection, medicine.
3. the culture medium raw material wide material sources be suitable for.The culture medium raw material that the present invention is suitable for can be that seed culture medium is commonly used in laboratory; When producing photosynthetic bacterium in enormous quantities, can city organic industrial sewage, industrial waste, agricultural byproducts waste be raw material after comprehensive treating process, after inoculating photosynthetic bacterium, carry out fermentation culture; In pharmaceutical sector etc., application, can analytical pure inorganic salts chemical reagent be raw material, make substratum, inoculation, cultivates, purifies, makes photosynthetic bacterium product.
4. suppress varied bacteria growing.Sorbic Acid can mould fungus inhibition, yeast and aerobic spoilage organism grow, and does not suppress photosynthetic bacterium growth, and especially in acid condition, the Sorbic Acid of 0.02mmol/L can play bacteriostatic action; With the organic waste water processed by fermentation for raw material carry out photosynthetic bacterium cultivate time, can varied bacteria growing be suppressed, promote photosynthetic bacterium growth, make photosynthetic bacterium become dominant microflora, play larger water purification or accumulate the effect of active substance.
5. thalline adaptability is good.The present invention all has growth promoting function to commercially available multiple Photosynthetic bacterium strain, does not have particular requirement, use safety, to eco-friendly to liquid nutrient medium.
Accompanying drawing explanation
Fig. 1 is that different concns photosynthetic bacterium growth promoter is to the promoter action figure of photosynthetic bacterium growth
Embodiment
Specific embodiments of the present invention is described in detail with reference to the accompanying drawings.
Embodiment 1
Follow these steps to operation:
(1) photosynthetic bacterium actication of culture (indoor):
Seed fermentation substratum was commonly used in preparation photosynthetic bacterium laboratory, loads in the transparent glass anaerobism bottle of 150ml capacity, through 115 DEG C of autoclavings 30 minutes.After cooling, strict aseptic technique, Rhodopseudomonas palustris (the Rhodopeudomonaspalustris culture presevation CGMCC1.2180) bacterial classification that access is bought, cultivate 3 ~ 5 days according to carrying out continuous illumination under 2500lux at incandescent light, culture temperature 28-32 DEG C, the obtained photosynthetic bacterium bacterial classification through overactivation.Test under microscope photosynthetic bacterial thallus is healthy and strong, motion is active, pollution-free.
The composed as follows of seed fermentation substratum is commonly used in described laboratory:
Ammonium chloride 1.0g, sodium-acetate 1.0g, magnesium sulfate 0.2g, sodium bicarbonate 1.0g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.2g, ironic citrate 0.005g, yeast extract paste 1.0g, peptone 1.0g, inorganic salts solution 10mL, growth cofactor 0.01g, distilled water 1000mL, PH=7.0.
Wherein grow cofactor to comprise: the microbial culture such as VITMAIN B1, amino-benzene methyl alcohol, vitamin H commonly use material.
Wherein inorganic salts solution refers to: the inorganic salts solution of trace, containing ironic citrate 5mg, calcium sulfate 0.05mg, boric acid 1mg, manganous chloride 0.05mg, zinc sulfate 1mg in often liter of distilled water.
(2) different concns photosynthetic bacterium growth promoter is to the promoter action (indoor) of photosynthetic bacterium growth:
In laboratory conditions, carry out photosynthetic bacterium growth promoter and add the preferred of concentration.Preparation commonly uses seed fermentation substratum containing the photosynthetic bacterium laboratory of different concns photosynthetic bacterium growth promoter, and add in the transparent glass anaerobism bottle of 250ml capacity respectively, loading amount is 180ml, through 115 DEG C of autoclavings 30 minutes, cools for subsequent use.
The one-tenth of different concns photosynthetic bacterium growth promoter is grouped into sees design table 1, establish 11 process altogether, be numbered 1,2,3,4,5,6,7,8,9,10,11(contrast), by the photosynthetic bacterium bacterial classification that activated according to 10% inoculum size, being linked into the laboratory containing photosynthetic bacterium growth promoter cooled commonly uses in seed fermentation substratum, carry out continuous illumination cultivation (temperature 28-32 DEG C) 4 days at incandescent light according under 2500lux, measure photosynthetic bacterium bacterium number.
Table 1
In test, different concns photosynthetic bacterium growth promoter compound method is:
Weigh Sorbic Acid and the ethanol of calculated amount shown in table 1, put into beaker, stirred at ambient temperature 3 minutes, mixed dissolution, add photosynthetic bacterium laboratory and commonly use in seed fermentation substratum, abundant stirring and dissolving.
Test-results: as shown in table 2, Fig. 1.
Fig. 1 be different concns photosynthetic bacterium growth promoter to the promoter action figure of photosynthetic bacterium growth, ordinate zou is bacterium number (hundred million/ml), and X-coordinate is different photosynthetic bacterium concentration.
Table 2
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11(contrasts) |
| Bacterium number (hundred million/ml) | 16.2 | 16.8 | 18.7 | 19.8 | 20.3 | 19.8 | 19.3 | 17.7 | 16.8 | 16.6 | 15.1 |
Test-results (table 2) shows, test the growth that each concentration Sorbic Acid and alcohol mixture all can promote photosynthetic bacterium, the span that wherein bacterium number is higher is 0.01 ~ 0.5mmol/L(3 ~ No. 8 process), the span that bacterium number is higher is 0.03 ~ 0.1mmol/L(4 ~ No. 7 process), wherein add additive capacity (0.05mmol/L) bacterium number with No. 5 process the highest.
Embodiment 2
Operation can be followed these steps to the present invention:
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) method is with embodiment 1 (2).
Difference is, the one-tenth of the photosynthetic bacterium growth promoter added is grouped into sees design table 3,
Add the ethanol (its add-on is shown in design table 3) of different concns, establish 7 process altogether, be numbered 1,2,3,4,5,6,7(contrast).
Table 3
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Sorbic Acid (mmol/L) | 0.05 | 0.08 | 0 | 0 | 0 | 0 | 0 |
| Potassium sorbate (mmol/L) | 0 | 0 | 0.05 | 0.08 | 0 | 0 | 0 |
| Sodium sorbate (mmol/L) | 0 | 0 | 0 | 0 | 0.05 | 0.08 | 0 |
| Ethanol (mmol/L) | 0.15 | 0.20 | 0.15 | 0.20 | 0.15 | 0.20 | 0 |
Test-results: as shown in table 4.
Test-results shows, the mixture of Sorbic Acid, potassium sorbate, sodium sorbate and the ethanol adding 0.05mmol/L or 0.08mmol/L in seed culture medium is respectively commonly used in laboratory, all can promote the growth of photosynthetic bacterium, improves photosynthetic bacterium bacterium number.
Table 4
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Bacterium number (hundred million/ml) | 19.7 | 19.2 | 19.9 | 20.5 | 20.4 | 20.8 | 16.3 |
Embodiment 3
Operation can be followed these steps to the present invention:
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) photosynthetic bacterium growth promoter cultivates the impact (in laboratory conditions) on growth under different pH condition: working method is with embodiment 1 (2);
Difference is, the composition that the photosynthetic bacterium growth promoter added in seed culture medium is commonly used in laboratory consists of the Sorbic Acid of 0.05mmol/L and the ethanol of 0.2mmol/L, and adjust ph is respectively 6.5,7.0,7.5,8.0.
Test-results: the cultivation results of different pH value culture condition is as table 5.
Table 5
| PH value | 6.5 | 7.0 | 7.5 | 8.0 |
| Photosynthetic bacterium growth promoter (hundred million/ml) | 19.2 | 20.8 | 20.2 | 18.6 |
| Contrast (hundred million/ml) | 16.5 | 16.3 | 15.6 | 14.8 |
Test-results shows, pH value is in 6.5 ~ 8.0 scopes, and the interpolation Sorbic Acid of 0.05mmol/L and the ethanol of 9mmol/L all can promote photosynthetic bacterium growth in various degree, through the cultivation of 4 days, greatly improve cell concentration.
Embodiment 4
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) fermentation culture is expanded:
Conventional seed fermentation substratum (pH7.5) 10L in configuration laboratory, add the mixture of 0.015g potassium sorbate (relative to 0.1mmol/L) and 0.5ml ethanol, stirring and dissolving, method is with embodiment 1 (2), not add the conventional seed fermentation substratum of potassium sorbate and ethanol laboratory for contrast, by the photosynthetic bacterium bacterial classification of 30% inoculum size access through overactivation, load (cultivating base unit weight is 70%) in 5000 milliliters of transparent vials (also can adopt white plastic bucket), irradiate under being placed in nature sunlight and cultivate (within the scope of temperature 25-32 DEG C), within 8 days, expand fermentation culture ripe.
Result showed: through the cultivation of 8 days, and photosynthetic bacterial thallus cell quantity reaches more than 1,800,000,000/milliliter, and add the growth that Sorbic Acid can promote photosynthetic bacterium, the control group bacterium number more not adding Sorbic Acid is high by 17.8%.The motion of test under microscope photosynthetic bacterium is active, and thalline is healthy and strong, and phenomenon pollution-free, without exception, can be applicable to the fertilising of the crop such as vegetables or Chinese medicine, also can be used for livestock and poultry cultivation.
Embodiment 5
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) fermentation culture is expanded:
By photosynthetic bacterium seed fermentation nutrient solution (active photosynthetic bacterium number, every m1 reaches more than 1,000,000,000), by 20% be transferred to expand in fermention medium expand numerous, this expansion fermention medium selects beer waste water, be 6.5 ~ 7.0 by sodium hydroxide adjust ph, in 10 kg white plastic tanks, fill beer waste water 8 kilograms, often liter adds the potassium sorbate of 0.1mmol/L and the mixture of 0.5ml ethanol, is uniformly mixed dissolving.Cultivate under white plastic bucket after switching can be placed on nature solar irradiation, 10 days ripe, and its technical indicator can reach as table 6.
Table 6
Embodiment 6
Operation can be followed these steps to the present invention:
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) fermentation culture is expanded: by photosynthetic bacterium seed fermentation nutrient solution (containing active photosynthetic bacterium number, every m1 reaches 1,000,000,000), by 30% inoculum size be transferred to expand in fermention medium expand numerous, this expansion fermention medium selects pig farm organic waste water (pH7.0), pig farm organic waste water 7 kilograms is housed in 10 liters of white plastic buckets, often liter of mixture adding a certain amount of potassium sorbate (detailed addition is in table 7) and 1ml ethanol again, then (temperature 25 ~ 33 DEG C) is cultivated 10 days under being placed on nature solar irradiation, its photosynthetic bacterium content can reach as following table 7.
Table 7
Test-results shows, adds the growth that potassium sorbate is conducive to photosynthetic bacterium under complicated microbial environment.
The all commercially available acquisition of the photosynthetic bacterium bacterial classification adopted in the embodiment of the present invention, there is no particular requirement to it.
If use the soluble salt such as potassium sorbate, sodium sorbate in test, ethanol and sorbate directly can be added in photosynthetic bacterium liquid substratum, be stirred to abundant dissolving.
The selection principle expanding fermention medium when the present invention applies is:
In agricultural, poultry industry, environmental protection industry application, city organic industrial sewage, industrial waste, agricultural byproducts waste can be raw material after comprehensive treating process, then add additive; In pharmaceutical sector etc., application, then with analytical pure inorganic salts chemical reagent for raw material, should make substratum, inoculation, cultivates.
Additive of the present invention is for the photosynthetic bacterium bacterial classification of advantage, and its effect is more remarkable.
Claims (6)
1. one kind is impelled the growth promoter of Photosynthetic bacterium strain Fast-propagation, it is characterized in that, described growth promoter is: by weight by the one in 1 part of Sorbic Acid, potassium sorbate or sodium sorbate or mixture, join in 1-4 part ethanol, stirring, mixed dissolution process, obtain this product growth promoter;
The temperature of described stirring, mixed dissolution process is-10 ~ 45 DEG C, and the time is 1 ~ 10min;
Described ethanol is medical 75-95% ethanol.
2. impel a using method for the growth promoter of Photosynthetic bacterium strain Fast-propagation, it is characterized in that,
Joined by growth promoter in the photosynthetic bacterium liquid substratum prepared, be stirred to abundant dissolving, the photosynthetic bacterium bacterial classification of the activation of access 10-30%, loads in airtight or semi-open light transmission container, at 25 ~ 32 DEG C, and 1500-6000lux illumination cultivation;
The concentration adding the sorb acid group of growth promoter in described photosynthetic bacterium liquid substratum controls within the scope of 0.005 ~ 1.0mmol/L.
3. a kind of using method impelling the growth promoter of Photosynthetic bacterium strain Fast-propagation as claimed in claim 2, is characterized in that,
The concentration adding the sorb acid group of growth promoter in described photosynthetic bacterium liquid substratum is: 0.03 ~ 0.1mmol/L.
4. a kind of using method impelling the growth promoter of Photosynthetic bacterium strain Fast-propagation as claimed in claim 2, is characterized in that,
Described photosynthetic bacterium liquid substratum refers to that the city organic industrial sewage of seed fermentation substratum or comprehensive treating process in the usual way, agricultural byproducts waste or industrial waste are commonly used in laboratory.
5. a kind of using method impelling the growth promoter of Photosynthetic bacterium strain Fast-propagation as claimed in claim 4, it is characterized in that, it is composed as follows that seed fermentation substratum is commonly used in described laboratory:
Ammonium chloride 1.0g, sodium-acetate 1.0g, magnesium sulfate 0.2g, sodium bicarbonate 1.0g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.2g, ironic citrate 0.005g, yeast extract paste 1.0g, peptone 1.0g, inorganic salts solution 10mL, growth cofactor 0.01g, distilled water 1000mL, PH=6.5 ~ 8.0;
Wherein grow cofactor to comprise: one or more materials in VITMAIN B1, amino-benzene methyl alcohol, vitamin H;
Wherein inorganic salts solution refers to: containing ironic citrate 5mg, calcium sulfate 0.05mg, boric acid 1mg, manganous chloride 0.05mg, zinc sulfate 1mg in often liter of distilled water.
6. a kind of using method impelling the growth promoter of Photosynthetic bacterium strain Fast-propagation as claimed in claim 2, it is characterized in that, the preparation method of the photosynthetic bacterium bacterial classification of described activation is:
Seed fermentation substratum is commonly used in the photosynthetic bacterium laboratory prepared, load in the transparent glass anaerobism bottle of 150ml capacity, through 115 DEG C of autoclavings 30 minutes, after cooling, strict aseptic technique, access Rhodopseudomonas palustris Rhodopeudomonaspalustris bacterial classification, culture presevation number is CGMCC1.2180,1500-6000lux illumination cultivation 3-5 days, culture temperature 28-32 DEG C, the photosynthetic bacterium bacterial classification of obtained activation.
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| CN102992894A (en) * | 2012-11-20 | 2013-03-27 | 蒋常德 | Preparation method for high-concentration photosynthesis bacterial manure |
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