CN102992894A - Preparation method for high-concentration photosynthesis bacterial manure - Google Patents

Abstract

The invention discloses a high-concentration photosynthetic bacterial fertilizer in the technical field of microorganisms and a preparation method thereof. The photosynthetic bacterial fertilizer medium of the present invention is composed of ammonium chloride, sodium acetate, sodium chloride, sodium carbonate, magnesium chloride, potassium dihydrogen phosphate, yeast Paste, brown sugar, sodium α-naphthalene acetate, sodium nitrophenolate, potassium sorbate, natural water, etc. The present invention adds potassium sorbate to inhibit the growth of miscellaneous bacteria, so as to achieve the high concentration of natural water to cultivate photosynthetic bacteria, photosynthetic bacteria The concentration can exceed 10 billion/ml. The α-naphthalene sodium acetate and sodium nitrophenolate added in the present invention can not only promote the rapid reproduction of photosynthetic bacteria as a culture medium, but also promote the rapid rooting and growth of plants after they are applied to the field, so that they can be added once to play a double role. Moreover, the compounding of photosynthetic bacteria, α-naphthyl acetate and sodium nitrophenolate on plant growth is far greater than the effect of a single substance. It can prevent disease, fertilize, degrade drug residues, repair soil, and use less. Quick effect.

Description

A kind of preparation method of high-concentration photosynthetic bacteria fertilizer

technical field

The invention belongs to the technical scope of functional microbial bacterial fertilizer, and in particular relates to a preparation method of high-concentration photosynthetic bacterial fertilizer.

Background technique

Photosynthetic bacteria can promote the transformation of soil substances, improve soil structure, improve soil fertility, and promote crop growth. Most photosynthetic bacteria have the ability to fix nitrogen and can increase soil nitrogen levels. Through their metabolic activities, they can effectively increase certain organic components, sulfide, and ammonium nitrogen in the soil, and promote the transformation of harmful pollutants (such as pesticides, fertilizers). At the same time, it can promote the proliferation of beneficial microorganisms, so that they can participate in the material cycle of soil ecology. In addition, the abundant physiologically active substances produced by photosynthetic bacteria such as proline, uracil, cytosine, vitamins, coenzyme Q, carotenoids, etc. can be directly absorbed by plants, which helps to improve crop nutrition and activate the activity of plant cells , Promote root development, improve photosynthesis and reproductive growth. The fermentation broth of photosynthetic bacteria contains a variety of physiologically active substances, such as chlorophyll A, B, carotenoids, multivitamins (especially B vitamins), and various plant hormones (such as IAA, GA3, ABA, ethylene, cytokinin, etc.) ), nucleic acids, salicylic acid, various amino acids, and single-cell proteins. These physiologically active substances can either promote the growth of plant roots, or promote the growth of plant leaves, or promote the accumulation of certain specific components in fruits, or increase crop yields, thereby improving the quality of agricultural products. Photosynthetic bacteria can enhance the ability of crops to resist and prevent diseases. Photosynthetic bacteria contain anti-bacterial and anti-viral substances, which can inactivate the pathogenicity of pathogens and inhibit the growth of pathogens. At the same time, the activity of photosynthetic bacteria can promote the proliferation of beneficial microorganisms such as actinomycetes and inhibit the growth of harmful bacteria such as filamentous fungi, thereby effectively inhibiting the occurrence and spread of certain plant diseases. Because photosynthetic bacteria have the function of disease resistance and disease prevention, they can prolong the storage period of fruits. Photosynthetic bacteria can increase the chlorophyll content of plants, and promote the growth of microbial flora such as nitrogen-fixing bacteria, rhizobia, and actinomycetes in the soil. After spraying photosynthetic bacteria on crops such as tobacco, radish, sweet potato, and soybean, it can increase nitrogen-fixing bacteria in the soil. Bacteria, rhizobia, actinomycetes and bacteria, while filamentous fungi are reduced, playing a role in soil restoration. Photosynthetic bacteria can also reduce the toxicity of pesticides to crops and degrade residual pesticides. Therefore, photosynthetic bacteria have broad application prospects in planting.

However, the existing photosynthetic bacteria have a low bacterial content (less than 5 billion/ml), and they are used in a large amount as bacterial fertilizers, and the effect is slow. Generally, they are diluted 50-100 times for spraying or root irrigation. The stability is poor, and the use conditions are relatively harsh. The effect is obvious when it is used above 20 degrees. Moreover, the water for cultivating photosynthetic bacteria in existing documents and patents is mainly distilled water or purified water, or the culture medium needs to be sterilized, the operation process is complicated, and the energy consumption is high.

Contents of the invention

Aiming at the deficiencies of the prior art, the present invention provides a method for preparing a high-concentration photosynthetic bacterial fertilizer with fast bacterial growth, less bacteria, high fertilizer activity, high stability, and fast effect.

The technical solution adopted in the present invention is: a preparation method of high-concentration photosynthetic bacteria fertilizer, characterized in that the raw materials of the culture medium of photosynthetic bacteria fertilizer are: 1-1.8 parts of ammonium chloride, 6-18 parts of sodium acetate , 2-30 parts of sodium chloride, 1-3 parts of sodium carbonate, 0.1-0.3 parts of magnesium chloride, 0.2-05 parts of potassium dihydrogen phosphate, 0.2-0.5 parts of yeast extract, 0.2-0.4 parts of brown sugar, 0.1 parts of sodium α-naphthalene acetate -0.2 parts, 0.1-0.2 parts of sodium nitrophenolate, 0.1-0.2 parts of potassium sorbate, 1000 parts of natural water, etc.

The preparation method of high-concentration photosynthetic bacteria fertilizer of the present invention:

Add in order to the water tank or bucket: 1-1.8 parts of ammonium chloride, 6-18 parts of sodium acetate, 2-30 parts of sodium chloride, 1-3 parts of sodium carbonate, 0.1-0.3 parts of magnesium chloride, 0.2 parts of potassium dihydrogen phosphate -05 parts, 0.2-0.5 parts of yeast extract, 0.2-0.4 parts of brown sugar, 0.1-0.2 parts of sodium α-naphthalene acetate, 0.1-0.2 parts of sodium nitrophenolate, 0.1-0.2 parts of potassium sorbate, 1000 parts of natural water, etc. Stir to dissolve completely, add 150-250 parts of bacteria, adjust the pH to 6.5 with acetic acid, pack into 5L transparent glass bottles or transparent plastic barrels, the filling volume accounts for 95% of the bottle volume, and cover tightly with rubber stoppers; Cultivate at 20-40 degrees for 3-5 days, stir once a day, and the light intensity is 1500-6000lex. The effect of cultivating in natural sunlight is better, and no external light source is needed at night. When △OD660 (using the medium solution as Blank) is greater than 7.0, and the detection of live bacteria is greater than 10 billion/ml, the finished product can be packaged, or it can continue to be used as a strain to expand the culture again.

The present invention screens out 11 culture medium components from more than 40 kinds of photosynthetic bacteria culture medium components, considers the mutual influence of 11 culture medium components, uses 11 factors and 5 levels of tests to optimize the culture medium, and optimizes the concentration of photosynthetic bacteria after cultivation It can exceed 12 billion/ml, which is much higher than the culture concentration of existing photosynthetic bacteria.

The beneficial effect of adopting the above-mentioned technical scheme: the high-concentration photosynthetic bacteria fertilizer contains potassium sorbate as a food preservative, and potassium sorbate can effectively inhibit the activity of mould, yeast and aerobic bacteria, and can also prevent botulism, The growth and reproduction of harmful microorganisms such as Staphylococcus and Salmonella, but it is almost ineffective against beneficial microorganisms such as photosynthetic bacteria and Lactobacillus acidophilus. Its inhibitory effect on growth is stronger than its bactericidal effect. The role of bacteria.

The high-concentration photosynthetic fertilizer contains sodium α-naphthalene acetate, which is a broad-spectrum plant growth conditioner, which can rapidly promote cell division and expansion (fruit swelling agent, swelling factor), and induce the formation of adventitious roots (rooting agent), which can regulate growth, promote rooting, germination, flowering, prevent flower and fruit drop, form seedless fruit, promote early maturity, increase production, etc., and can also enhance the drought resistance, cold resistance, disease resistance, salt and alkali resistance, and drought resistance of plants. Hot air capacity. It is a broad-spectrum, high-efficiency, low-toxic plant growth conditioner. the

The high-concentration photosynthetic bacteria fertilizer contains sodium nitrophenolate, which is a powerful cell rejuvenation agent. After contacting with plants, it can quickly penetrate into the plant body, promote the flow of protoplasm of cells, and improve cell vitality. It can speed up the growth rate, break dormancy, promote growth and development, prevent flower drop, fruit cracking, and fruit shrinkage, improve product quality, increase yield, and improve crop resistance to disease, insects, drought, waterlogging, cold, salt, and alkali. Lodging and other resistance. It is widely used in food crops, economic crops, melons and fruits, vegetables, fruit trees, oil crops and flowers. It can be used at any time between plant sowing and harvest, for seed dipping, seedbed filling, foliar spraying and bud spreading, etc. Because of its advantages of high efficiency, low toxicity, no residue, wide application range, no side effects, and wide concentration range, it has been popularized and applied in many countries and regions in the world. Sodium nitrophenolate is also used in animal husbandry and fishery. While increasing the output and quality of meat, eggs, wool, and skins, it can also enhance animal immunity and prevent various diseases.

The culture medium of the high-concentration photosynthetic bacteria fertilizer contains two kinds of plant growth regulators, sodium α-naphthalene acetate and sodium nitrophenolate, which not only have the above-mentioned advantages for plants, but also can promote the rapid division of photosynthetic bacteria cells, Combined with the antiseptic and antibacterial effects of potassium sorbate, natural water can be directly used to cultivate photosynthetic bacteria, which can achieve low miscellaneous bacteria and high concentration of photosynthetic bacteria. In the initial cultivation stage of photosynthetic bacteria fertilizer, when the pH value is relatively low, photosynthetic bacteria will compete with miscellaneous bacteria in natural water, but in the presence of potassium sorbate, other harmful bacteria and miscellaneous bacteria will be inhibited, and α - Sodium naphthalene acetate and sodium nitrophenolate promote the rapid division of photosynthetic bacteria and accelerate their reproduction. With the reproduction of photosynthetic bacteria, the pH will continue to rise, and the high pH will lead to the death of other bacteria. In the end, other bacteria Basically gone.

The α-naphthalene sodium acetate and sodium nitrophenolate added in the present invention can not only promote the rapid reproduction of photosynthetic bacteria as a culture medium, but also promote the rapid rooting and growth of plants after they are applied to the field, so that they can be added at one time to play a double role. , and photosynthetic bacteria combined with α-naphthyl acetate and sodium nitrophenolate, its effect on plant growth is far greater than the effect of a single substance, which can prevent disease, fertilize, degrade drug residues, use less, and get quick results , the effect is stable. The photosynthetic bacterial fertilizer prepared by the preparation method can be continuously expanded and cultivated as strains, has simple operation and low equipment investment, is suitable for farmers to cultivate themselves, and can be widely popularized.

The field effect of high-concentration photosynthetic bacterial fertilizer of the present invention is illustrated below in conjunction with test.

1. Application effect of high-concentration photosynthetic fertilizer on tobacco

1. Test location: a farmer's planting site in Longzhu Village, Tantou Town, Longhui County, Hunan Province

2. Test method: Flue-cured tobacco variety: Zhongyan 99-10-1. When transplanting flue-cured tobacco seedlings, use 500 times of high-concentration photosynthetic fertilizer to irrigate the roots once; Once, and then continuously spray the second and third times every 20 days (a total of 4 kg of high-concentration photosynthetic bacteria fertilizer per mu). The control area was sprayed with the same amount of water while spraying with 250 times of bacterial fertilizer each time;

3. test results:

After the application of high-concentration photosynthetic bacteria fertilizer, the plant height, stem girth, pitch, number of effective leaves, and maximum leaf area were all greater than those of the control sprayed with water, which indicated that the agronomic traits of the tobacco plants could be effectively improved and the robust growth of the tobacco plants could be promoted. The morbidity and disease degree of the applied tobacco plants are significantly reduced, and the disease resistance of the tobacco plants can be effectively improved. After the application of high-concentration photosynthetic bacteria fertilizer, the tobacco matures earlier, improves the quality, and greatly increases the yield, average price, output value, and the proportion of high-to-medium tobacco leaves. From Table 1, it can be concluded that the output, output value, and average price of tobacco leaves in the experimental demonstration area are 38.1 kg/mu, 774.6 yuan/mu, and 1.24/kg higher than the control area, respectively. Subtracting the increased cost of 350 yuan (including 310 yuan for labor), a net increase of 424.6 yuan/mu can be achieved. The application of the photosynthetic bacterial fertilizer product can increase the output per mu of tobacco leaves by 19.2%, and the net output value of tobacco leaves per mu increases by 30.9%.

Table 1 Effect of high-concentration photosynthetic fertilizer on tobacco yield and value increase

deal with Yield (kg/mu) Output value (yuan/mu) Average price (yuan/kg) control area 198.2 2505.2 12.64 processing area 236.3 3279.8 13.88

Two , the test effect of high concentration photosynthetic bacterial fertilizer on paddy rice

1. Test location: a farmer's farmland in Longzhu Village, Tantou Town, Longhui County, Hunan Province

2. experiment method:

⑴. Soak the seeds with 100 times high-concentration photosynthetic fertilizer dilution solution for 2-4 hours;

⑵. At the seedling stage, tillering stage, and booting stage, spray once with a 250-fold dilution of high-concentration photosynthetic bacterial fertilizer at 1000 ml/mu;

3. test results:

⑴. The effect on the germination rate of rice is the best. It can promote seed germination, rooting, and increase germination potential and germination rate. The germination potential increases by 5.4-7.8%, and the germination rate increases by 6.3-8.5%. Germination is fast and neat, the root system is developed, there are many thick white roots, and the seedling rate is high;

⑵. It has obvious effects on rice seedling raising, field growth and development, and improvement of per unit yield, especially on improving seedling quality, high survival seedlings, and increasing thousand-grain weight. According to different application periods in the plot test, the rice yield can be increased by 30-60 kg per mu, the yield increase range is 5-8%, the increased income is 60-120 yuan, the cost is deducted by 40 yuan, and the net increased income per mu is 20-80 yuan;

⑶. Improve drought resistance, disease resistance, lodging resistance and other stress resistance of rice.

3. Application effect of high-concentration photosynthetic fertilizer on apples

1. Test location: Qiansuo Town, Suizhong County, Liaoning Province

2. Test method: The test is divided into: control area, that is, the area where high-concentration photosynthetic bacteria fertilizer is not used. The test area is an area where high concentration of photosynthetic fertilizer is used. The two areas applied the same other fertilizers and other agricultural operations, and the same water was used for the control. Experimental area: in spring, dilute the high-concentration photosynthetic bacterial fertilizer 500 times and apply it in the water or in holes. Spray once with a 250-fold dilution of high-concentration photosynthetic bacteria fertilizer during the apple flowering period and fruit expansion period (a total of 5 kg per mu);

3. test results

After using foliar spraying in the flowering period, the survey found that the fruit setting rate of apples was significantly increased. Compared with the control, the fruit setting rate in the test area increased by 8-9%; The number of abnormalities was significantly reduced, and the first-grade fruit applied with high-concentration photosynthetic fertilizer was 12% higher than that in the control area when harvesting, and the fruit size was relatively uniform, and the yield was significantly increased. The yield in the experimental area was 14% higher than that in the control area; more importantly The most important thing is that the color of the fruit is uniform, the surface is smooth, the brightness increases, the sugar content increases by 2-3 Baume degrees, and the shape and taste are better.

4. Experimental effect of high-concentration photosynthetic fertilizer on strawberries

1. Test location: a grower in Taohong Town, Longhui County, Hunan Province

2. Test method: During the planting period, the seedlings are planted, and the roots are irrigated once with a 500-fold dilution of high-concentration photosynthetic bacterial fertilizer.

During the growth period of strawberries, spray 1:250 times high-concentration photosynthetic bacteria fertilizer on the leaves, once every 10 days (days), and spray 3 times;

3. test results:

It was found that early flowering and fruiting, large and plump fruits, many fruits, less abnormal-shaped fruits, good taste and bright coloring, the average yield per plant of strawberry (Fengxiang) was 118.4g, which was higher than that of the control (no photosynthetic fertilizer) 19.07% and the soluble solid content of strawberry fruit after spraying the bacterial fertilizer reached 9.82%, 11.9% higher than that of the control. At the same time, after the bacterial fertilizer was applied, the acid content decreased, the Vc content increased, and the flavor and quality of the fruit were significantly improved. And it is storage-resistant, and it can be stored for more than 10 days than strawberries that do not use bacterial fertilizer. The yield of plots using high-concentration photosynthetic bacterial fertilizer increased by 24% compared with the control area, and the sugar content increased by 1-2 degrees compared with the control area. the

5. Application test of high-concentration photosynthetic fertilizer on muskmelon

1. Test time: February-June 2011

2. Test site: a grower's greenhouse in Cang County, Hebei Province

3. Test method: use 100 times of high-concentration photosynthetic fertilizer to soak for 2 hours; 10 days after emergence, spray with 250 times of bacterial fertilizer for the first time, and then spray for the second to fourth time every 20 days continuously ( A total of 4 kg of bacterial fertilizer per mu);

4. Test results:

⑴. Yield increased and quality improved. The yield per mu of melon with bacterial fertilizer reached 4498 kg, which was 13.4% higher than that of the control; the weight of a single melon was 1.36 kg, which was 0.114 kg higher than that of the control; the refraction sugar content was 16.4, which was 1.22 higher than that of the control;

⑵. Less investment, high benefit. Bacterial manure increases investment by 40 yuan per mu, labor cost increases by 100 yuan, and the total increase is 140 yuan, which can increase net income by about 300 yuan, and the input-output ratio is 1:2.

6. Application effect of high-concentration photosynthetic fertilizer on tomato

1. Test time: June-September 2011

2. Test location: a grower in Dulin Township, Cang County, Hebei Province

3. Test method: Before transplanting, soak the roots of the seedlings with a 200-fold dilution of bacterial fertilizer for 30 minutes, and irrigate the roots with a 500-fold dilution of bacterial fertilizer 10 days after planting. During the growth of tomatoes, spray 1: 250 times of bacterial fertilizer, once every 20 days, spray 3 times;

4. Test results: It was found that early flowering and fruiting, large and plump fruits, many fruiting numbers, few shaped fruits, good taste and bright coloring, the average plant height of tomatoes increased by 9% compared with the control (without spraying bacterial fertilizer), and the yield increased by 18% compared with the control %. the

Detailed ways

The substantive features of the present invention can be realized from the following examples, but these examples are only for illustration, not to limit the present invention.

Example 1

The preparation method of this high-concentration photosynthetic bacteria fertilizer:

Add 1.2 parts of ammonium chloride, 8 parts of sodium acetate, 12 parts of sodium chloride, 1.6 parts of sodium carbonate, 0.2 parts of magnesium chloride, 0.3 parts of potassium dihydrogen phosphate, 0.3 parts of yeast extract, and 0.3 parts of brown sugar in a bucket or tank , 0.15 parts of sodium α-naphthalene acetate, 0.15 parts of sodium nitrophenolate, 0.2 parts of potassium sorbate, 1000 parts of natural water, etc. Stir to dissolve completely, add 200 parts of bacteria, adjust the pH to 6.5 with acetic acid, pack into 5L transparent glass bottles or plastic barrels, the liquid volume is 95% of the volume of the bottles, and cover tightly with rubber stoppers; place in 20 Cultivate at -40 degrees for 4 days, stir once a day, light intensity 1500-6000lex, cultivate under natural sunlight, no light source at night, △OD660 (medium solution as blank) is equal to 7.9, viable bacteria detection 12.1 billion/ml , the finished product can be packaged.

Example 2

The preparation method of this high-concentration photosynthetic bacteria fertilizer:

Add in order to the bucket or tank: 1.0 parts of ammonium chloride, 6 parts of sodium acetate, 20 parts of sodium chloride, 1.2 parts of sodium carbonate, 0.2 parts of magnesium chloride, 0.3 parts of potassium dihydrogen phosphate, 0.2 parts of yeast extract, 0.2 parts of brown sugar , 0.2 parts of sodium α-naphthalene acetate, 0.2 parts of sodium nitrophenolate, 0.2 parts of potassium sorbate, 1000 parts of natural water, stir and dissolve completely, add 150 parts of bacteria, adjust the pH to 6.5 with acetic acid, and pack it into 5L Transparent glass bottle or plastic bucket, the liquid volume is 95% of the volume of the bottle, and it is tightly closed with a rubber stopper; it is cultivated at 20-40 degrees for 4 days, stirred once a day, and the light intensity is 1500-6000lex. Cultivate under the sun, without light source at night, △OD660 (medium solution as blank) is equal to 7.1, and the viable bacteria detection is 10.5 billion/ml, then the finished product can be packaged.

Claims (5)

1. the preparation method of a high density photosynthetic bacterial fertilizer, the raw material that it is characterized in that the substratum of photosynthetic bacterial fertilizer is by weight: ammonium chloride 1-1.8 part, sodium-acetate 6-18 part, sodium-chlor 2-30 part, yellow soda ash 1-3 part, magnesium chloride 0.1-0.3 part, 0.2-05 part of potassium primary phosphate, yeast extract paste 0.2-0.5 part, brown sugar 0.2-0.4 part, α-naphthaleneacidsodium 0.1-0.2 part, sodium nitrophenolate 0.1-0.2 part, potassium sorbate 0.1-0.2 part, 1000 parts of grades of Natural Water composition.

2. the preparation method of a kind of high density photosynthetic bacterial fertilizer according to claim 1, the preparation process that it is characterized in that high density photosynthetic bacterial fertilizer of the present invention: in water vat or bucket, add successively: ammonium chloride 1-1.8 part, sodium-acetate 6-18 part, sodium-chlor 2-30 part, yellow soda ash 1-3 part, magnesium chloride 0.1-0.3 part, 0.2-05 part of potassium primary phosphate, yeast extract paste 0.2-0.5 part, brown sugar 0.2-0.4 part, α-naphthaleneacidsodium 0.1-0.2 part, sodium nitrophenolate 0.1-0.2 part, potassium sorbate 0.1-0.2 part, 1000 parts of Natural Waters, stirring and dissolving is complete, the bacterial classification that adds 150-250 part, transfer pH to 6.5 with acetic acid, divide the clear-glass bottle or the plastics oil drum that are filled to 5L, loading amount account for the bottle volume 95%, cover tightly with rubber plug; Place under the 20-40 degree and cultivated 3-5 days, stir once every day, intensity of illumination is 1500-6000lex, culture effect is better under the natural sun, do not need external light source evening yet, as △ OD660(with culture medium solution as blank) greater than 7.0, viable bacteria detects greater than 10,000,000,000/milliliter, get final product packaging final prod, also can continue as bacterial classification enlarged culturing again.

3. the preparation method of a kind of high density photosynthetic bacterial fertilizer according to claim 1, the raw material that it is characterized in that the best medium of photosynthetic bacterial fertilizer is by weight: 1.2 parts of ammonium chlorides, 8 parts of sodium-acetates, 12 parts in sodium-chlor, 1.6 parts in yellow soda ash, 0.2 part in magnesium chloride, 0.3 part of potassium primary phosphate, 0.3 part of yeast extract paste, 0.3 part in brown sugar, 0.15 part of α-naphthaleneacidsodium, 0.15 part of sodium nitrophenolate, 0.2 part of potassium sorbate, 1000 parts of grades of Natural Water composition.

4. the preparation method of a kind of high density photosynthetic bacterial fertilizer according to claim 1 is characterized in that α-naphthaleneacidsodium, and it is the high purity α-naphthaleneacidsodium.

5. the preparation method of a kind of high density photosynthetic bacterial fertilizer according to claim 1 is characterized in that Natural Water, and it is well water, wherein a kind of of river, pond water etc.