CN103181463A - Sheep selenium-containing microecological feed additive and preparation method thereof - Google Patents
Sheep selenium-containing microecological feed additive and preparation method thereof Download PDFInfo
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- CN103181463A CN103181463A CN 201110445199 CN201110445199A CN103181463A CN 103181463 A CN103181463 A CN 103181463A CN 201110445199 CN201110445199 CN 201110445199 CN 201110445199 A CN201110445199 A CN 201110445199A CN 103181463 A CN103181463 A CN 103181463A
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- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 48
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 48
- 239000011669 selenium Substances 0.000 title claims abstract description 48
- 241001494479 Pecora Species 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000003674 animal food additive Substances 0.000 title abstract 4
- 241000894006 Bacteria Species 0.000 claims abstract description 15
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000000047 product Substances 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 8
- 241000228212 Aspergillus Species 0.000 claims abstract description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 7
- 239000004310 lactic acid Substances 0.000 claims abstract description 7
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 7
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 7
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 7
- 239000007787 solid Substances 0.000 claims abstract description 6
- 239000012265 solid product Substances 0.000 claims abstract description 5
- 244000068988 Glycine max Species 0.000 claims abstract description 4
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 4
- 240000008042 Zea mays Species 0.000 claims abstract description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 4
- 235000005822 corn Nutrition 0.000 claims abstract description 4
- 229940065287 selenium compound Drugs 0.000 claims abstract description 3
- 150000003343 selenium compounds Chemical class 0.000 claims abstract description 3
- 239000000654 additive Substances 0.000 claims description 9
- 230000000996 additive effect Effects 0.000 claims description 9
- 230000000813 microbial effect Effects 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 229940088598 enzyme Drugs 0.000 claims description 7
- 239000004459 forage Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 5
- 102000008114 Selenoproteins Human genes 0.000 claims description 4
- 108010074686 Selenoproteins Proteins 0.000 claims description 4
- 238000009472 formulation Methods 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 235000015099 wheat brans Nutrition 0.000 claims description 4
- 241000235342 Saccharomycetes Species 0.000 claims description 3
- RJFAYQIBOAGBLC-UHFFFAOYSA-N 2-amino-4-methylselanyl-butanoic acid Chemical compound C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 claims description 2
- 239000004382 Amylase Substances 0.000 claims description 2
- 102000013142 Amylases Human genes 0.000 claims description 2
- 108010065511 Amylases Proteins 0.000 claims description 2
- 108010059892 Cellulase Proteins 0.000 claims description 2
- 102000035195 Peptidases Human genes 0.000 claims description 2
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 claims description 2
- 108010012715 Superoxide dismutase Proteins 0.000 claims description 2
- 235000019418 amylase Nutrition 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 229940106157 cellulase Drugs 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 230000001079 digestive effect Effects 0.000 claims description 2
- 230000007269 microbial metabolism Effects 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000003651 drinking water Substances 0.000 claims 1
- 235000020188 drinking water Nutrition 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 6
- 230000004060 metabolic process Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract 2
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 239000000969 carrier Substances 0.000 abstract 1
- 229940079919 digestives enzyme preparation Drugs 0.000 abstract 1
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 230000001850 reproductive effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 206010053759 Growth retardation Diseases 0.000 description 2
- 206010039921 Selenium deficiency Diseases 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 231100000001 growth retardation Toxicity 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000014860 sensory perception of taste Effects 0.000 description 2
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 206010058359 Hypogonadism Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 241001417949 Psychrolutidae Species 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical group [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000004641 brain development Effects 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 210000002390 cell membrane structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000000527 lymphocytic effect Effects 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
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- 210000000496 pancreas Anatomy 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000003161 proteinsynthetic effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
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- 230000003612 virological effect Effects 0.000 description 1
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention provides a sheep selenium-containing microecological feed additive which can improve animal body metabolism and a preparation method thereof. The feed additive is characterized in that an organic selenium compound is added to a culture solution containing soybean cake hydrolysate and corn steep liquor, and then vaccinated with active yeast, lactic acid bacteria, bacillus, and aspergillus for liquid fermentation. As the fermentation progresses, the added bacteria multiply, the added organic selenium is gradually transformed into organic selenium proteins and thus a selenium-containing liquid protein product is prepared. Then bran and other carriers are added and yeast is vaccinated for solid fermentation to produce a solid product. The product of the invention includes a lot of beneficial live bacterium preparations, beneficial enzyme preparations and selenium-rich proteins. The product used as a sheep microecological feed additive can significantly increase metabolism of sheep, promote healthy and rapid growth of sheep, significantly improve sheep reproductive rate, and play a role on improving disease resistance of sheep.
Description
1. technical field
The present invention relates to fowl poultry microbial forage additive field, specifically a kind of sheep is with containing selenium microbial forage additive and preparation method thereof.After the product that utilizes this method to make enters in the sheep body, can play and promote digestion, promotion absorbs, promotes the metabolic effect of sheep to selenium, also can play vegetative cell simultaneously, prevent cell impaired, make up good micro-ecological environment in the sheep body, promote that the absorption of nutriment transforms, improve immunity of organisms, promote the healthy growth fast of sheep, can also significantly improve the sheep breeding potential, and the raising of sheep premunition is had effect.
2. technical background
Selenium is that humans and animals carries out the requisite a kind of trace element of normal activities, and especially a lot of important enzyme in the human body all needs the participation of selenium could synthesize and guarantee normally carrying out of metabolism.The researcher carries out the diet research in nine weeks by a definite date at the male sex sportsman of 12 two teens, allows experimental group absorb the selenium of 16 micrograms every day, and control group has only the selenium of 2 micrograms every day.The researcher points out, when the selenium picked-up is not enough, just can't discharge the carbon dioxide that accumulates in the health effectively, also can't allow oxygen exchange fully in vivo, makes completely long-pending metabolic refuse in the health, naturally will be more and more tired.China people's diet structure is with the cereal master, and the bioavailability of selenium is very low in cereal, is 20-35% only, if children eat purified diet more, wherein the content of selenium is lost too much, the easier selenium deficiency that causes.
Each system of whole body all can be adversely affected during selenium deficiency.Especially to puberty gonad maturity influence more direct.The lad of The Nile, Egypt delta suffers from when lacking selenium disease, and main performance is growth retardation and hypogonadism.Confirm after deliberation that in Iran maiden and school-ager have growth retardation and the slow person of sexal maturity, food is strengthened selenium after, patient is grown is tending towards normal.Zoopery confirms, lacks the animal brain depauperation of selenium.Some tissue of eyeball lacks selenium, will influence photochemistry, makes eyesight become undesired.Selenium is again the composition of insulin, and the selenium in the pancreas is reduced to a half of normal contents, and the possibility of diabetes is just arranged.Have the selenium of denier to distribute in the overwhelming majority tissue, wherein liver, muscle and bone content are higher.Selenium is the main component of tens of kinds of enzymes in the body.Selenium is also relevant with brain development and intelligence.University of the U.S. finds that the interior selenium content of teenager's body clever, that succeed in school is all than fathead height.Selenium promotes the effect of lymphopoiesis and mobility in addition, cutaneous lesions such as epithelium and mucosal tissue are normal to keeping, defense against bacterial, viral intrusion, promotion wound healing, minimizing acne, and the malfunctioning grade of the correction sense of taste all has magical effect.
3. summary of the invention
In view of the important function of selenium for the humans and animals vital movement, the invention provides and to improve the metabolic a kind of sheep of animal body with containing selenium microbial forage additive and preparation method thereof.After the product that utilizes this method to make enters in the sheep body, can play and promote digestion, promotion absorbs, promotes the metabolic effect of animal to selenium, make up good micro-ecological environment in the sheep body, promote the absorption of nutriment to transform, improve immunity of organisms, promote the healthy growth fast of sheep.Its key character is at first to add organic selenium compounds in the nutrient solution that contains soya-bean cake hydrolyzate and corn steep liquor, inserts active yeast, lactic acid bacteria, bacillus, Aspergillus again and carries out liquid fermentation.Along with the carrying out of fermentation, the bacterial classification of adding is bred in a large number, the organic selenium that adds is also gradated be organic selenoprotein, namely makes the liquid protein product that contains selenium.And then add carrier such as wheat bran and insert the saccharomycete bacterial classification and carry out solid fermentation, make solid product.Contain a large amount of useful active bacteria formulations, useful enzyme preparation in this invention product and contain the abundant albumen of selenium, can significantly improve the metabolism of sheep as the sheep microbial forage additive, promote the healthy growth fast of sheep, can also significantly improve simultaneously the breeding potential of sheep, and the raising of sheep premunition is had effect.
For achieving the above object, a large amount of useful active bacteria formulation of the present invention comprises active yeast, lactic acid bacteria, bacillus, Aspergillus; Active biological enzyme comprises proteolytic enzyme, amylase, contains selenium digestive ferment, superoxide dismutase, cellulase; Contain zymoprotein, selenium methionine that participation protein active division center that selenoprotein forms for the organic selenium that adds transforms forms after microbial metabolism, contain the compound of formation such as selenium polypeptide.
For achieving the above object, preparation method of the present invention, its key character is:
(1) soya-bean cake hydrolyzate, corn steep liquor and sterilized water are according to percentage (10-14): (4-5): (81-86) be configured to fluid nutrient medium earlier, carry out the high-temperature boiling sterilization;
(2) fluid nutrient medium behind boiling sterilization is respectively charged in the round, and seals behind organic selenium material of adding percentage (0.5-0.8);
(3) get the preferred bacterial classification of cultivating that is in logarithmic phase and inoculate, active yeast, lactic acid bacteria, bacillus, Aspergillus bacterium liquid is precentagewise (8-12) respectively: (5-8): (3-5): (70-80) add.Adjusting the pH value is (7.0-7.3), and temperature is (28-32 ℃), Continuous Cultivation (7) sky;
(4) after the end of liquid fermentation stage, liquid fermentation material and wheat bran are (through high-temperature sterilization, and spreading for cooling to room temperature) add according to the ratio of percentage by weight (1: 1.4), insert saccharomycete bacterium liquid (2-5) % simultaneously and carry out solid fermentation, incubation time be (4) sky;
(5) the solid fermentation stage mainly is to utilize wheat bran to contain selenium microbial liquid product to make solid product as carrier absorption.Can be observed after solid fermentation finishes and be covered with the white hypha body in the material, after dehydrating, can be made into and contain selenium microbial solid product.
4. advantage of the present invention
(1) selenium in this product can participate in the synthetic of the interior plurality of enzymes of sheep body, is conducive to metabolism and normally carries out and can promote growing;
(2) keep the normal sense of taste of sheep and appetite, regulate V
AMetabolism;
(3) can promote lymphocytic division and cell to change, keep the immunologic function of cell;
(4) promote protein synthetic, accelerate cell division and breeding, but and the stabilized cell membrane structure;
(5) power of regeneration of enhancing wound tissue is accelerated reparation, the healing of wound tissue.
Claims (4)
1. a kind of sheep of the present invention is with containing selenium microbial forage additive and preparation method thereof, and its key character is that it contains a large amount of useful active bacteria formulations, useful enzyme preparation and contain the abundant albumen of selenium; Useful active bacteria formulation wherein comprises active yeast, lactic acid bacteria, bacillus, Aspergillus; Active biological enzyme comprises proteolytic enzyme, amylase, contains selenium digestive ferment, superoxide dismutase, cellulase; Contain zymoprotein, selenium methionine that participation protein active division center that selenoprotein forms for the organic selenium that adds transforms forms after microbial metabolism, contain the compound of formation such as selenium polypeptide.
2. additive for microbe feedstuff as claimed in claim 1, it is characterized in that the living bacterial cells sum in the product reaches 2,500,000,000/ml-35, hundred million/ml, based on active yeast, lactic acid bacteria, it accounts for total viable count (70-80) %, and bacillus, Aspergillus account for (20-30) % of total viable count respectively.
3. additive for microbe feedstuff as claimed in claim 1 is characterized in that the addition in the feed is (0.2-0.4) % of feed gross weight, and the addition in drinking-water is (0.01-0.02) % of water weight.
4. a kind of sheep of the present invention is with containing selenium microbial forage additive and preparation method thereof, it is characterized in that at first in the nutrient solution that contains soya-bean cake hydrolyzate and corn steep liquor, adding organic selenium compounds, insert active yeast, lactic acid bacteria, bacillus, Aspergillus again and carry out liquid fermentation.Along with the carrying out of fermentation, the bacterial classification of adding is bred in a large number, the organic selenium that adds is also gradated be organic selenoprotein, namely makes the liquid protein product that contains selenium.And then add carrier such as wheat bran and insert the saccharomycete bacterial classification and carry out solid fermentation, make solid product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110445199 CN103181463A (en) | 2011-12-28 | 2011-12-28 | Sheep selenium-containing microecological feed additive and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110445199 CN103181463A (en) | 2011-12-28 | 2011-12-28 | Sheep selenium-containing microecological feed additive and preparation method thereof |
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| Publication Number | Publication Date |
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| CN103181463A true CN103181463A (en) | 2013-07-03 |
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| CN 201110445199 Pending CN103181463A (en) | 2011-12-28 | 2011-12-28 | Sheep selenium-containing microecological feed additive and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105707901A (en) * | 2016-02-04 | 2016-06-29 | 王植 | Activated selenium and production method thereof |
| CN109496992A (en) * | 2018-12-04 | 2019-03-22 | 湖南袁禾农业科技有限公司 | A kind of production method of super selenium-rich yellow meal worm |
| CN112913782A (en) * | 2021-03-16 | 2021-06-08 | 新疆农垦科学院 | Method for improving reproductive performance of sheep |
| CN114947014A (en) * | 2022-06-09 | 2022-08-30 | 宁夏九盛牧业科技研究院(有限公司) | Additive premix capable of reducing fat of Tan sheep carcass and preparation method thereof |
-
2011
- 2011-12-28 CN CN 201110445199 patent/CN103181463A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105707901A (en) * | 2016-02-04 | 2016-06-29 | 王植 | Activated selenium and production method thereof |
| CN105707901B (en) * | 2016-02-04 | 2019-03-05 | 王植 | Active selenium and its production method |
| CN109496992A (en) * | 2018-12-04 | 2019-03-22 | 湖南袁禾农业科技有限公司 | A kind of production method of super selenium-rich yellow meal worm |
| CN109496992B (en) * | 2018-12-04 | 2021-05-25 | 湖南袁禾农业科技有限公司 | A kind of production method of super selenium-enriched Tenebrio molitor |
| CN112913782A (en) * | 2021-03-16 | 2021-06-08 | 新疆农垦科学院 | Method for improving reproductive performance of sheep |
| CN114947014A (en) * | 2022-06-09 | 2022-08-30 | 宁夏九盛牧业科技研究院(有限公司) | Additive premix capable of reducing fat of Tan sheep carcass and preparation method thereof |
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Application publication date: 20130703 |