CN103091431A - Coating apparatus for immobile phase in capillary column - Google Patents
Coating apparatus for immobile phase in capillary column Download PDFInfo
- Publication number
- CN103091431A CN103091431A CN201110334660XA CN201110334660A CN103091431A CN 103091431 A CN103091431 A CN 103091431A CN 201110334660X A CN201110334660X A CN 201110334660XA CN 201110334660 A CN201110334660 A CN 201110334660A CN 103091431 A CN103091431 A CN 103091431A
- Authority
- CN
- China
- Prior art keywords
- capillary column
- coating liquid
- closed container
- coating
- coating apparatus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011248 coating agent Substances 0.000 title claims abstract description 53
- 238000000576 coating method Methods 0.000 title claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 40
- 230000005526 G1 to G0 transition Effects 0.000 claims description 4
- 230000010412 perfusion Effects 0.000 abstract description 9
- 238000007789 sealing Methods 0.000 abstract description 4
- 238000007872 degassing Methods 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 239000002904 solvent Substances 0.000 description 4
- 210000004907 gland Anatomy 0.000 description 3
- 238000001291 vacuum drying Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000002309 gasification Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Paints Or Removers (AREA)
Abstract
The invention discloses a coating apparatus for an immobile phase in a capillary column. The coating apparatus comprises a closed container for holding a coating liquid, wherein the top of the closed container is provided with an insert connection port, the insert connection port position is filled with an elastic sealing plug, the elastic sealing plug is provided with a through hole for insertion of a capillary column, and the inner wall of the through hole and the outer wall of the capillary column requiring processing form an interference match. With the coating apparatus, degassing and perfusion of the coating liquid can be completed in one time so as to increase capillary column immobile phase coating success rate to more than 95%, and the formed immobile phase has good thickness uniformity.
Description
Technical field
The present invention relates to a kind of perfusion in the capillary column, apply the fixedly device of phase.
Background technology
Capillary chromatographic column is applied in the analyzing and testing field widely, capillary chromatographic column mainly is comprised of capillary column and the related accessory of glass or metal material, wherein the inwall of capillary column is coated with fixedly phase, and its performance has critical impact to detecting effect.
Add man-hour and be generally fixing phased soln or be dispersed in and be configured to coating liquid in organic solvent, then coating liquid is fed in capillary column, then make fixedly through vacuum drying that the phase material is covering at the capillary column inwall.
In prior art, coating liquid is dissolved with a small amount of air, if do not processed the film that is difficult to form the thickness homogeneous after fixing drying mutually, makes to apply success ratio only in 10% left and right.
Summary of the invention
The invention provides a kind of air that can remove in coating liquid, and can disposablely complete the coating unit of coating liquid perfusion.
A kind of coating unit of stationary phase in capillary column, comprise the closed container for the splendid attire coating liquid, this closed container top is provided with interface, this interface place is filled with the resilient seal plug, this resilient seal is provided with beyond the Great Wall for the through hole that inserts capillary column, and interference fit between the outer wall of the inwall of described through hole and capillary column to be processed.
coating liquid when coating unit of the present invention uses in the heating closed container, and the end that capillary column to be processed inserts in closed container is positioned on the liquid level of coating liquid, rising along with temperature, in coating liquid, the air of dissolving can be discharged gradually, discharge another port by capillary column to be processed, the basic emptying of air of dissolving in coating liquid after the coating liquid boiling, be inserted into the end that capillary column to be processed inserts in closed container under the liquid level of coating liquid this moment, the coating liquid solvent of gasification raises the closed container internal pressure, then coating liquid is pressed in capillary column to be processed, also just completed the perfusion of coating liquid.
After completing perfusion, capillary column to be processed is in the outer port sealing of closed container, again capillary column to be processed is extracted closed container, make solvent evaporates in coating liquid by vacuum drying at last, and residue forms fixedly phase at capillary column inwall to be processed.
Coating unit of the present invention can disposablely complete the degassed of coating liquid and the perfusion, with capillary column fixedly the coating success ratio of phase be increased to more than 95%, the fixedly phase thickness homogeneity of formation is good.
Description of drawings
Fig. 1 is the structural representation of coating unit of the present invention;
Fig. 2 is that the coating unit in Fig. 1 is completed the structural representation when pouring into after degassed.
Embodiment
Referring to accompanying drawing, the coating unit of a kind of stationary phase in capillary column of the present invention, comprise the closed container 1 for splendid attire coating liquid 2, this closed container 1 top is provided with interface, this interface place is filled with resilient seal plug 4, resilient seal plug 4 is provided with for the through hole that inserts capillary column 5, interference fit between the outer wall of the inwall of through hole and capillary column to be processed 5.
Closed container 1 top can also be provided with gland bonnet 3, and during use, gland bonnet 3 is closed, the coating liquid 2 of being convenient to annotate when gland bonnet 3 is opened.
Guarantee good impermeability after capillary column 5 inserts, resilient seal plug 4 can adopt can aitiogenic material with coating liquid, as tetrafluoroethene etc.
Coating liquid when coating unit of the present invention uses in the heating closed container, and the end that capillary column to be processed inserts in closed container is positioned on the liquid level of coating liquid, rising along with temperature, in coating liquid, the air of dissolving can enter capillary column along the arrow A direction, discharges by the another port of capillary column.
The basic emptying of air of dissolving in coating liquid after the coating liquid boiling, be inserted into the end that capillary column to be processed inserts in closed container under the liquid level of coating liquid this moment, the coating liquid solvent of gasification raises the closed container internal pressure, the arrow B direction is exerted pressure to the liquid level of coating liquid, then coating liquid is pressed in capillary column along the arrow C direction, has also just completed the perfusion of coating liquid.
After completing perfusion, capillary column is in the outer port sealing of closed container, then capillary column to be processed is extracted closed container, make solvent evaporates in coating liquid by vacuum drying at last, and residue forms fixedly phase at capillary column inwall to be processed.
Coating unit of the present invention can disposablely complete the degassed of coating liquid and the perfusion, with capillary column fixedly the coating success ratio of phase be increased to more than 95%, the fixedly phase thickness homogeneity of formation is good.
Claims (1)
1. the coating unit of a stationary phase in capillary column, comprise the closed container for the splendid attire coating liquid, it is characterized in that, this closed container top is provided with interface, this interface place is filled with the resilient seal plug, this resilient seal is provided with beyond the Great Wall for the through hole that inserts capillary column, and interference fit between the outer wall of the inwall of described through hole and capillary column to be processed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110334660XA CN103091431A (en) | 2011-10-31 | 2011-10-31 | Coating apparatus for immobile phase in capillary column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110334660XA CN103091431A (en) | 2011-10-31 | 2011-10-31 | Coating apparatus for immobile phase in capillary column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103091431A true CN103091431A (en) | 2013-05-08 |
Family
ID=48204258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110334660XA Pending CN103091431A (en) | 2011-10-31 | 2011-10-31 | Coating apparatus for immobile phase in capillary column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103091431A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103995072A (en) * | 2014-05-10 | 2014-08-20 | 宁波市成大机械研究所 | Gas-phase capillary glass tubular column filling method and apparatus |
| CN111097560A (en) * | 2019-11-22 | 2020-05-05 | 安徽工业大学 | A kind of pipette tip based on secondary molding, preparation method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS633260A (en) * | 1986-06-24 | 1988-01-08 | Kurita Water Ind Ltd | chromatography equipment |
| JPH04323557A (en) * | 1991-04-23 | 1992-11-12 | Hitachi Plant Eng & Constr Co Ltd | Device for filling column for chromatography |
| CN101105482A (en) * | 2007-07-27 | 2008-01-16 | 福州大学 | Novel device made of monolithic column of capillary electrochromatography and its application method |
| CN202305492U (en) * | 2011-10-31 | 2012-07-04 | 天津市科密欧化学试剂有限公司 | Device for coating stationary phase in capillary column |
-
2011
- 2011-10-31 CN CN201110334660XA patent/CN103091431A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS633260A (en) * | 1986-06-24 | 1988-01-08 | Kurita Water Ind Ltd | chromatography equipment |
| JPH04323557A (en) * | 1991-04-23 | 1992-11-12 | Hitachi Plant Eng & Constr Co Ltd | Device for filling column for chromatography |
| CN101105482A (en) * | 2007-07-27 | 2008-01-16 | 福州大学 | Novel device made of monolithic column of capillary electrochromatography and its application method |
| CN202305492U (en) * | 2011-10-31 | 2012-07-04 | 天津市科密欧化学试剂有限公司 | Device for coating stationary phase in capillary column |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103995072A (en) * | 2014-05-10 | 2014-08-20 | 宁波市成大机械研究所 | Gas-phase capillary glass tubular column filling method and apparatus |
| CN111097560A (en) * | 2019-11-22 | 2020-05-05 | 安徽工业大学 | A kind of pipette tip based on secondary molding, preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2014060293A3 (en) | Sealing apparatus and method | |
| WO2009149458A8 (en) | Compression sealing apparatus and method for use of the same | |
| CN106363070B (en) | A kind of processing technology of metal bi multiple tube | |
| CN106840535A (en) | Engine cylinder-body flaw detection device | |
| CN103091431A (en) | Coating apparatus for immobile phase in capillary column | |
| CN204422286U (en) | The telescopic mounting of pressure pipeline gas sample | |
| WO2010051912A3 (en) | Leak testing of a pressure vessel | |
| CN202305492U (en) | Device for coating stationary phase in capillary column | |
| CN202704235U (en) | Automobile tire repair glue bottle | |
| CN207101968U (en) | A kind of chromatography column apparatus of automatic liquid-feeding | |
| CN106932284B (en) | A contact surface large deformation shear seepage test device and its use method | |
| CN204570241U (en) | A kind of bowl cover for adding liquid auxiliary agent | |
| CN103091432A (en) | Method for coating stationary phase in capillary column | |
| CN206411134U (en) | The repeatable vapour-pressure type ELISA sampling devices utilized | |
| CN203275035U (en) | Vacuum freezing drier board layer leakage detection device | |
| CN202372317U (en) | Device for detecting overall leak rate in accumulated way | |
| CN102814259B (en) | Sensor housing silicone grease injection apparatus | |
| CN206258227U (en) | Engine cylinder-body flaw detection device | |
| JP2017129363A (en) | Oil injection device and oil injection method | |
| CN103395583B (en) | A storage tank manhole operation well | |
| CN205027611U (en) | Battery sealing washer vapour -pressure type pressure measurement | |
| CN220399005U (en) | A negative pressure sampling device | |
| CN204332609U (en) | The few oily equipment live quantitative/recharging oil device of electric power | |
| CN204855100U (en) | Oil duct leak test plugging device | |
| CN104990944A (en) | Oilfield sewage pipeline microbiological detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130508 |