CN103091384B - Prefabricated agarose gel electrophoresis sample loading buffer and use method thereof - Google Patents

Abstract

A preloaded agarose gel electrophoresis loading buffer and a use method thereof belong to the technical field of agarose gel electrophoresis. The buffer solution includes several drops of concentrated sample buffer solution, plastic seal, and tongue seal. The plastic seal is made of low-density polyethylene plastic folded and tightly bonded. Each drop of concentrated sample buffer solution is evenly injected and sealed at the crease of the plastic seal. , a few drops of concentrated sample loading buffer are evenly dispersed and sealed at the crease of the plastic seal, and a sealing tongue is provided on the edge of the plastic seal. When using, suck the nucleic acid sample into the pipette according to the calculation, adjust the pipette to increase the suction volume by one drop of buffer solution, and then suck one drop of buffer solution to achieve the purpose of mixing evenly and loading the sample. The invention performs sample loading without repeated measurement of concentrated sample loading buffer solution, and the operation is simple and easy to learn, saving time and effort.

Description

Preloaded agarose gel electrophoresis loading buffer and its usage

technical field

The invention relates to a prepacked agarose gel electrophoresis sample loading buffer and a use method thereof, belonging to the technical field of agarose gel electrophoresis.

Background technique

Agarose gel electrophoresis is a common technique in molecular biology experiments and is generally used for the detection, separation and purification of nucleic acid samples. In the sample loading step of agarose gel electrophoresis, the usual practice is: measure a certain volume of concentrated loading buffer, mix the nucleic acid sample with the concentrated loading buffer according to a certain ratio, and then use a micro-sampler to The mixture is added to the wells of the gel. Since the volumes of various liquids involved in this step are generally less than 10 μl, and the concentrated loading buffer needs to be repeatedly measured, the operation is cumbersome, time-consuming and labor-intensive.

Contents of the invention

The object of the present invention is to provide a kind of preloaded agarose gel electrophoresis loading buffer and using method, by means of this prepacked agarose gel electrophoresis loading buffer, it can be convenient and quick during agarose gel electrophoresis to load nucleic acid samples.

The preloaded agarose gel electrophoresis loading buffer provided by the present invention is characterized in that the preloaded agarose gel electrophoresis loading buffer includes several drops of concentrated loading buffer, plastic seal, tongue seal, The plastic seal is made of low-density polyethylene plastic folded and tightly bonded. Each drop of concentrated sample buffer solution is evenly injected and sealed at the crease of the plastic seal, and several drops of concentrated sample buffer are evenly dispersed and sealed at the crease of the plastic seal. , there is an easy-to-tear plastic seal on the edge of the plastic seal, see Figure 1.

There are 6-12 drops of drop-shaped concentrated loading buffer sealed in the plastic package of the prepacked agarose gel electrophoresis loading buffer.

The drop-shaped concentrated loading buffer solution has a volume of 1-2 μl (preferably 1 μl), and the distance between two adjacent drops is greater than 1 cm. The distance is greater than 1cm.

When using the prepacked agarose gel electrophoresis loading buffer, follow the steps below: ①Pinch the flap and gently tear off the plastic seal, spread it flat on the laboratory bench, and unfold the prepacked agarose gel electrophoresis The schematic diagram of the loading buffer is shown in Figure 2; ②According to the concentration multiple and volume of the drop-shaped concentrated loading buffer, use a pipette to absorb the nucleic acid sample solution, and the volume of the nucleic acid sample solution is the volume of the drop-shaped concentrated loading buffer × ( Concentration ratio of concentrated sample loading buffer - 1); ③Adjust the volume adjustment knob of the pipette to increase the volume of the set volume by one drop of concentrated sample loading buffer, press the control button of the pipette to remove the nucleic acid in the tip Slowly push the sample solution to the top of the tip, move the tip into the drop-shaped concentrated loading buffer and slowly release the control button to inhale the concentrated loading buffer; ④Press the control button to the bottom, blow out the sample solution and concentrated Loading buffer, then loosen the control button to suck back the sample solution and concentrated loading buffer to achieve the purpose of mixing; ⑤ Add the mixed sample solution and concentrated loading buffer to the gel hole of the agarose gel in electrophoresis.

The loading buffer of the present invention may comprise a plurality of tearable flaps. In general, multiple drops of buffer solution are required for sample loading. Cut out the required number of drops according to the needs, and tear the seal to load the sample, so as not to waste materials. Compared with the above-mentioned sample loading process of traditional agarose gel electrophoresis, using the present invention for sample loading does not need to repeatedly measure the concentrated sample loading buffer solution, the operation is simple and easy to learn, and saves time and effort.

Description of drawings

Fig. 1 is the schematic diagram of undeveloped preloaded agarose gel electrophoresis loading buffer;

Fig. 2 is the schematic diagram of the preloaded agarose gel electrophoresis loading buffer developed;

In the figure 1, concentrated loading buffer solution, 2, plastic seal, 3, tongue seal.

Detailed ways

The present invention will be further described below in conjunction with accompanying drawing and specific embodiment:

As shown in Figure 1, the preloaded agarose gel electrophoresis loading buffer is composed of a drop-shaped concentrated loading buffer 1, a plastic seal 2, and a sealing tongue 3. The plastic seal 2 is made of a rectangular low-density polyethylene plastic folded in half and tightly bonded. The drop-shaped concentrated loading buffer solution 1 is evenly injected into the crease of the plastic seal 2 and sealed. The edge of the plastic seal 2 is provided with a sealing tongue 3 that is easy to tear the plastic seal 2. There are 6-12 drops of concentrated sample loading buffer 1 sealed in the plastic package 2, each drop has a volume of 1-2 μl (preferably 1 μl), and the distance between two adjacent drops is greater than 1 cm, located on the drop-shaped concentration at the first and last positions The distance between sample buffer 1 and the edge of plastic package 2 is greater than 1cm.

When using this prepacked agarose gel electrophoresis loading buffer, follow the steps below: ①Pinch the flap 3 and gently tear off the plastic seal 2, and lay it flat on the test bench; ②Concentrate the loading buffer according to the drop shape Concentration multiple and volume of 1, use a pipette to absorb the nucleic acid sample solution with a volume of drop-shaped concentrated loading buffer × (concentration multiple-1); ③Adjust the volume adjustment knob of the pipette to increase the set capacity value One drop-shaped concentrated loading buffer volume, press the control button of the pipette to slowly push the nucleic acid sample solution in the tip to the tip of the tip, move the tip into the drop-shaped concentrated loading buffer 1 and slowly release the control button to inhale the concentrated loading buffer 1; ④ Press the control button to the bottom, blow out the sample solution and concentrated loading buffer 1 in the tip, and then release the control button to suck back the sample solution and concentrated loading buffer 1, reaching The purpose of mixing; ⑤ Load the mixed sample solution and concentrated loading buffer 1 into the gel holes of the agarose gel for electrophoresis.

Compared with the traditional agarose gel electrophoresis sample loading process, using this prepacked agarose gel electrophoresis sample loading buffer for sample loading does not need to repeatedly measure the concentrated sample buffer, the operation is simple and easy to learn, saving time and effort .

Claims (2)

1. prepackage type agarose gel electrophoresis sample-loading buffer, it is characterized in that, prepackage type agarose gel electrophoresis sample-loading buffer comprises several drop-wise and concentrates sample-loading buffer, plastic packaging, flap, plastic packaging is formed by LDPE (Low-density polyethylene plastics) doubling close adhesion, often drop-wise concentrates sample-loading buffer and evenly injects and be sealed in plastic packaging crease place, several drop-wise concentrate sample-loading buffer and are sealed in plastic packaging crease place dispersedly, are provided with the flap being easy to tear plastic packaging at plastic packaging edge; Be sealed with 6-12 drop-wise in plastic packaging and concentrate sample-loading buffer;

Drop-wise concentrates sample-loading buffer, and each volume dripped is 1-2 μ l, and the spacing of adjacent two is greater than 1cm, and the drop-wise being positioned at head and the tail position concentrates sample-loading buffer and is greater than 1cm apart from the distance at plastic packaging edge; Each volume dripped is 1 μ l.

2. the using method of prepackage type agarose gel electrophoresis sample-loading buffer according to claim 1, is characterized in that, comprise the following steps: 1. pinch flap and tear plastic packaging gently, lie on experiment table; 2. concentrate cycles of concentration and the volume of sample-loading buffer according to drop-wise, draw nucleic acid samples solution with pipettor, the volume of nucleic acid samples solution is that drop-wise concentrates sample-loading buffer volume × (concentrated sample-loading buffer cycles of concentration-1); 3. regulate the capacity regulating knob of pipettor to make it set capability value to increase one after another drop of shape and concentrate sample-loading buffer volume, nucleic acid samples solution in suction nozzle is slowly pushed to suction nozzle top by the control knob of pressing pipettor, suction nozzle is moved into drop-wise and to concentrate in sample-loading buffer and slowly to unclamp control knob to suck concentrated sample-loading buffer; 4. press control knob on earth, the sample solution in blowout suction nozzle and concentrated sample-loading buffer, then unclamp control knob to suck back sample solution and concentrated sample-loading buffer, reach the object of mixing; 5. mixed sample solution and concentrated sample-loading buffer are loaded onto in the glue hole of Ago-Gel and carry out electrophoresis.