CN103045467B - Biomedical Homogenizer - Google Patents

Abstract

The present invention provides a biomedical homogenization device comprising: the base body is provided with at least one power wheel, and the power wheel is arranged on the inner wall of the base body; the rotating assembly is rotatably assembled on the seat body and comprises a driving shaft and two rotating discs, the driving shaft is mutually assembled with the power wheel, two ends of the driving shaft are respectively provided with the two rotating discs, at least one rotating disc is provided with at least one driven wheel, and the power wheel drives the driven wheel to rotate relative to the rotating discs; and at least one accommodating component which is rotatably arranged between the two turntables and is mutually assembled with the driven wheel. The biomedical homogenizing device can increase the repeated impact frequency of the magnetic beads in the micro-tube at a fixed rotating speed so as to achieve the effect of improving the homogenization of the substances.

Description

Biomedical Homogenizer

technical field

The present invention relates to a biomedical homogenization device, in particular to a biomedical homogenization device which can enhance the homogenization effect and simultaneously have low temperature.

Background technique

In the field of molecular biology research, it is often necessary to extract the DNA, RNA or protein of various species to be tested or identified in order to carry out subsequent detection and observation of specific factors. Generally speaking, all kinds of tissues (such as skin, liver, kidney, etc.), cells, vaccines and other substances may be mixed with other impurities during the initial treatment, and even cannot be directly used due to the large volume of the tissue. Therefore, it is usually processed in a homogeneous manner, so that it can be applied to subsequent screening or purification procedures.

Traditional methods of homogenization can use high-speed centrifugation, ultrasonic vibration, or machine tool cutting. Among them, taking tissue homogenization as an example, the tissue fragments to be homogenized are usually placed in a microtube, and then cut by a machine tool with a blade on the head, so that the tissue fragments in the microtube are sucked into the microtube under high-speed operation. The blade part of the tool performs shearing and dispersing actions in the space gap, and generates high-pressure spray from the side of the head of the tool to quickly achieve the effect of micronizing tissue fragments. However, the method of cutting with the help of this traditional machine tends to produce a large number of tissue fragments remaining on the head of the machine, resulting in incomplete homogenization of the tissue fragments; moreover, it is more likely that due to careless cleaning of the head of the machine, Under the repeated use of the instrument, problems such as tissue fragment contamination and bacterial growth have occurred, which seriously affect the experimental quality of the tissue fragment in subsequent operations.

Therefore, at present, the homogenization of tissues, cells and other substances is mostly carried out by high-speed centrifugation, and high-speed centrifugal homogenizers have also been developed on the market, such as the Bullet BlenderTM homogenizer sold by Zhide International Co., Ltd. . Taking tissue homogenization as an example, at least one magnetic bead is placed in the microtube filled with the tissue to be homogenized, and then the microtube is placed in the slot provided by the homogenizer, and passed through The high-speed centrifugation of the homogenizer enables the magnetic beads to repeatedly hit the tissue to be homogenized in the microtube to achieve the purpose of tissue fragmentation and homogenization.

However, the above-mentioned high-speed centrifugal homogenizer can only drive the slot where the microtube is placed to rotate synchronously, so as to produce the effect of high-speed centrifugation. In this way, the frequency at which the magnetic beads repeatedly collide in the microtube is limited by the centrifugal speed of the homogenizer, and it is impossible to increase the repeated collision frequency of the magnetic beads in the microtube at a fixed speed, resulting in tissue homogenization. The effect is obviously limited; even, in the case of synchronous high-speed centrifugation, it is easy to make the magnetic beads fall to the bottom of the microtube, and only produce frequent micro-vibrations at the bottom of the microtube, which cannot hit the microtube comprehensively. The tissue to be homogenized in the microtubules seriously affects the homogenization effect of the tissue, as well as the experimental quality of the subsequent manipulation of the homogenized tissue for screening, purification or identification.

In view of this, it is indeed necessary to develop a biomedical homogenization device that can improve the homogenization effect, so as to solve the above-mentioned various problems through synchronous and independent high-speed centrifugation of the box containing the microtubes.

Contents of the invention

The main purpose of the present invention is to improve the above-mentioned shortcomings to provide a biomedical homogenization device, which can increase the frequency of repeated collisions of magnetic beads in microtubes at a fixed rotational speed, so as to improve the homogenization effect of substances.

The second object of the present invention is to provide a biomedical homogenization device, which can comprehensively impact the substance to be homogenized in the microtube, so as to achieve the effect of complete homogenization of the substance and maintain the quality of subsequent experiments.

Another object of the present invention is to provide a biomedical homogenization device, which can complete the homogenization operation in a low temperature environment, so as to improve the homogenization effect and the usability of the device.

In order to achieve the purpose of the aforementioned invention, the biomedical homogenization device of the present invention includes: a base body provided with at least one power wheel, and the power wheel is arranged on the inner wall of the base body; a rotating component is rotatably assembled on the base body , the rotating assembly includes a drive shaft and two turntables, the drive shaft and the power wheel are assembled with each other, the two ends of the drive shaft are respectively provided with the two turntables, and at least one turntable is provided with at least one driven wheel, the power wheel The driven wheel is driven to rotate relative to the turntable; and at least one accommodating component is rotatably arranged between the two turntables and assembled with the driven wheel.

The biomedical homogenization device of the present invention can be provided with two sets of power wheels on the base body, and the two sets of power wheels are respectively arranged on the corresponding inner walls of the base body, and each set of power wheels is stacked by two power wheels with the same number of teeth. combined. Moreover, a set of driven wheels is provided on the two turntables, and each set of driven wheels is composed of two driven wheels with the same number of teeth, and each set of driven wheels of the two turntables is mutually offset.

Wherein, the two ends of the drive shaft can pass through the two sets of power wheels respectively, and a power source is provided at one end of the drive shaft.

Wherein, the two turntables can be respectively engaged with each engaging portion of the two ends of the drive shaft, and are fixed by a buckle ring.

Furthermore, the driven wheel may have a rotating part and a connecting part, the rotating part may rotate relative to the turntable, the connecting part may be arranged on the inner surface of the rotating disk, and the receiving member may be provided by the connecting part Assembled to limit the accommodating member to be located between the two turntables.

Wherein, the place where the driven wheel is not provided on the two turntables is additionally provided with a set of joints, and the sets of joints can rotate relative to the turntable.

Wherein, one end of the accommodating member can be assembled with the assembling part of the driven wheel, and the other end of the accommodating member can be assembled with the assembling part of the turntable.

Wherein, a plurality of storage slots can be distinguished in the accommodation member, and the number storage slots are closed by a cover plate, and the cover plate is pivotally arranged on the periphery of the accommodation member.

In addition, the biomedical homogeneous device of the present invention can also be provided with a magnetic piece on the peripheral wall of the driving shaft, and the magnetic piece is wrapped around the peripheral wall of the driving shaft to provide magnetic force.

In addition, in the biomedical homogenization device of the present invention, an air circulation component can be further arranged in the base to control the air temperature inside the base, preferably maintaining a homogeneous environment at 4°C.

In the present invention, by means of the common connection configuration of the power wheel and the driven wheel, the rotating assembly can easily drive the accommodating member to rotate synchronously and independently, so as to increase the turnover frequency of the accommodating member at a stable rotational speed, thereby The number of repeated collisions of the magnetic beads in the microtube is increased, and the substance to be homogenized in the microtube can be comprehensively impacted to achieve the effect of enhancing the homogenization of the substance.

Description of drawings

Fig. 1 is a three-dimensional exploded view of the biomedical homogenization device of the present invention.

Fig. 2 is a schematic perspective view of the combined configuration of the biomedical homogenization device of the present invention.

Fig. 3 is a schematic diagram of some components of the biomedical homogenization device of the present invention.

Fig. 4 is a schematic cross-sectional view of the combined configuration of the biomedical homogenization device of the present invention.

Fig. 5 is a schematic diagram of the operation of the biomedical homogenization device of the present invention.

[Description of main component symbols]

〔this invention〕

1 seat body 11 power wheel 2 rotating assembly 21 driving shaft

211 Clamping part 212 Buckle 213 Magnetic piece 22 Turntable

221 assembly 23 driven wheel 231 rotating part 232 assembly

24 linkages 3 accommodation components 31 sets of connecting holes 32 storage slots

33 cover plate 4 microtube 41 magnetic beads 5 gas circulation components

6 adjustable controls S Accommodating space W Retrieval window B Open and close cover

M power source

Detailed ways

In order to make the above-mentioned and other objects, features and advantages of the present invention more comprehensible, the preferred embodiments of the present invention are specifically cited below, together with the accompanying drawings, as follows:

Please refer to FIG. 1, the biomedical homogenization device of the present invention includes a base 1, a rotating assembly 2 and at least one accommodating member 3, the rotating assembly 2 is rotatably assembled on the base 1, and the rotating assembly 2 Used to drive the rotation of the accommodating member 3 . Thereby, the number of microtubes 4 (as shown in FIG. 3 ) can be placed in the housing member 3, so that when the housing member 3 rotates synchronously and independently with the rotating assembly 2, the number of microtubes 4 can be increased. The magnetic beads 41 in the tube 4 collide with the frequency to achieve the effect of comprehensive fragmentation and homogenization of the material.

Wherein, the arrangement number of the housing members 3 determines the design type of the biomedical homogenization device of the present invention, so that each housing member 3 can produce synchronous rotation and independent rotation, and increase the volume of each microtube 4. The impact frequency of the magnetic beads 41 is the main principle. The accommodating member 3 can be at least one group, but the number of the accommodating member 3 is not limited. Placement of the tube 4, the present invention chooses to be provided with four accommodation components 3 in a symmetrical arrangement, and adopts the following design as a preferred embodiment of the present invention corresponding to the four accommodation components 3, please refer to Figures 1 to 5 shown in detail below.

Please refer to FIG. 1, which is a preferred embodiment of the present invention. The biomedical homogenization device includes a base 1, a rotating assembly 2 and four accommodating components 3. The rotating assembly 2 is rotatably assembled on the base. body 1, and the rotating assembly 2 is used to drive the rotation of each of the accommodating components 3.

The base body 1 is only used for supporting or accommodating the rotating assembly 2 , so the form of the base body 1 is not limited here. Especially as shown in FIG. 2 , the seat body 1 of this embodiment may have an accommodating space S for assembling the rotating assembly 2 in the accommodating space S. As shown in FIG. Moreover, a pick-up window W may be provided on the base body 1 , and the pick-up window W is preferably tightly sealed with an opening and closing cover plate B. Even, other equipment (for example: gas circulation components, etc.) can be optionally assembled on the base body 1, so that the biomedical homogenization device of the present invention can be widely used, or used in a low temperature environment.

Please refer to Figures 1 and 2, the base 1 is provided with at least one power wheel 11, the power wheel 11 can be driven by a power source, in this embodiment, the power source M can be a motor, the at least A power wheel 11 forms two groups and is respectively arranged on the corresponding inner wall of the base body 1 . The at least one set of power wheels 11 can be composed of two power wheels, and preferably two gears with the same number of teeth are superimposed on each other, so that when the rotating assembly 2 drives each of the accommodating members 3 to rotate, each of the accommodating members 3 can be made to rotate. Set member 3 to carry out the homogenization operation at the same centrifugal speed.

The rotating assembly 2 is rotatably assembled on the base body 1 , preferably assembled between the two sets of power wheels 11 . The rotating assembly 2 of this embodiment includes a drive shaft 21 and two turntables 22, the drive shaft 21 is assembled with the two sets of power wheels 11, especially the two ends of the drive shaft 21 respectively penetrate through the two sets of power wheels 11, And one end of the driving shaft 21 is provided with a power source M, so that the power source M outputs power to drive the rotation of the rotating assembly 2 . Moreover, the two ends of the drive shaft 21 are respectively provided with the two turntables 22, and the junction of the two turntables 22 and the drive shaft 21 can form a non-circular shape, so that the two can rotate synchronously. In this embodiment, Two ends of the drive shaft 21 are respectively provided with a clamping portion 211 for the two turntables 22 to be socketed, and are fixed by a buckle 212 to ensure that the two turntables 22 can rotate synchronously with the drive shaft 21 .

In addition, in the present invention, a magnetic piece 213 can also be installed on the outer peripheral wall of the driving shaft 21. As shown in the drawing of FIG. to attract the magnetic beads 41 in the microtube 4 . Wherein, the arrangement form of the magnetic member 213 is optional but not limited to completely covering the drive shaft 21 , which can be easily understood by those skilled in the art, and will not be described in detail here.

Referring again to FIG. 1 , the present invention optionally but not limitedly provides at least one driven wheel 23 on one of the turntables 22 in the rotating assembly 2 , so as to engage the accommodating member 3 by means of the driven wheel 23 . The driven wheel 23 can be driven by the power wheel 11 and rotate relative to the rotating disk 22. The driving method can be various existing driving methods, such as: using gears to mesh between the two for indirect transmission; A ring of internal teeth is arranged on the outer periphery of 22, and the driven wheel 23 meshes with the internal teeth; or as shown in this embodiment, the driven wheel 23 and the power wheel 11 are driven to rotate by linkages 24 such as chains and belts. In this embodiment, the two turntables 22 are preferably provided with a set of driven wheels 23 (that is, a set of two driven wheels with the same number of teeth (Module), hereinafter referred to as "driven wheels 23" for short), and the two The driven wheels 23 of the turntables 22 are preferably arranged in a mutual misalignment, especially as shown in the drawing of FIG. , it is possible to leave a more appropriate distance between each of the accommodating members 3, so as to avoid affecting the rotation effect during centrifugation. And, the place where the driven wheel 23 is not provided with the two rotating disks 22 is provided with an array of connectors 221, which can be positioned and rotated relative to the rotating disk 22. The driven wheels 23 on the turntable 22 correspond to each other and are used for receiving one end of the receiving member 3 .

Wherein, each of the driven wheels 23 in the driven wheels 23 has a rotating part 231 and a connecting part 232 respectively, the rotating part 231 can rotate relative to the turntable 22, and the connecting part 232 is preferably protruded on the The inner surface of the rotating disk 22 is used to assemble the other end of the receiving member 3 by the joint part 232, and restrict the receiving member 3 to be located between the two rotating disks 22, and make the rotating part 231 of each driven wheel 23 and The power wheels 11 of the base body 1 correspond to each other to be engaged with each other by a link 24 .

In order to adapt to the configuration of multiple sets of power wheels 11 and multiple sets of driven wheels 23 in this embodiment, the rotating assembly 2 of the biomedical homogenization device of the present invention is selected to be provided with a number of linkages 24, and the number of linkages 24 are respectively arranged around the The power wheel 11 and the driven wheel 23 drive the power wheel 11 and the driven wheel 23 to rotate by the circular movement of the link 24, especially because the number of teeth of the power wheel 11 is greater than the number of teeth of the driven wheel 23, and the driven wheel 23 can be forced to rotate. Driving wheel 23 is done the rotation of more number of revolutions.

Please refer to Figures 1 and 3, each of the containing components 3 is rotatably arranged between the two turntables 22, preferably a set of connecting holes 31 are provided on the corresponding two side walls of each of the containing components 3, The assembly holes 31 at one end of each of the accommodation members 3 are assembled on the assembly portion 232 of the driven wheel 23, and the assembly holes 31 at the other ends of each of the accommodation members 3 are assembled on the assembly of the turntable 22. The member 221 makes the rotation of the accommodating member 3 more balanced and stable. Wherein, the shape and size design of the containing member 3 are only based on the principle of being able to hold several microtubes 4 and being pivotally arranged between the two rotating disks 22 of the rotating assembly 2, so the containing member 3 is not limited here. form as it should be presented.

Particularly, as shown in FIG. 3 , the accommodating member 3 is formed into a box shape, and several cavities 32 are distinguished in the box-shaped accommodating member 3 , and the diameter width of each cuvette 32 matches the diameter of the microtube 4. It is a better principle to design the width of the diameter so that the microtube 4 can be accommodated in the container 32 without excessive left and right shaking in the container 32, which can be easily understood by those skilled in the art, and is no longer More on that. And, the number capacity groove 32 of this accommodating member 3 is closed with a cover plate 33, and this cover plate 33 is preferably pivotally arranged on the periphery of this accommodating member 3, so that it is easy to open and close, especially can reach more when closing. The fastening effect is good, so that the cover plate 33 will not be loosened during high-speed centrifugation and the microtube 4 will not fall.

It is worth noting that the biomedical homogenization device of the present invention can bear the above, and a gas circulation component 5 (as shown in FIG. The air temperature in the seat 1 enables the biomedical homogenization device of the present invention to provide different homogenization environments, preferably maintained at a low temperature of 4°C, so as to be widely applicable to various animal tissues, cells or bacteria species. Moreover, an adjustment control 6 can be arranged on the outer wall of the base 1, so that the menu can be controlled by the panel of the adjustment control 6, and the high-speed centrifugal speed of the biomedical homogenization device of the present invention in the homogenization process can be mastered, as well as various animal tissues. , The ambient temperature suitable for cells or strains, to achieve a better effect of material homogenization.

Please refer to Figures 4 and 5, which are only one form of the biomedical homogenization device of the present invention configured with the base 1, the rotating assembly 2 and the number of accommodating members 3, so as to illustrate the application of the present invention in the homogenization process. Action situation.

Before operating the biomedical homogenization device of the present invention, the material to be homogenized (for example: animal tissue, cells or bacterial species, etc.) is first selected to be added to the microtube 4, and at least A magnetic bead 41 (as shown in FIG. 3 ), and then several microtubes 4 that have been configured are placed in the cavities 31 of each accommodating member 3, and the appropriate rotation speed and temperature are set through the control 6 , to carry out the homogenization operation at the same time. In this way, the drive shaft 21 of the rotary assembly 2 is driven to rotate by means of the power source M outputting power, so that the number accommodating member 3 arranged between the two turntables 22 of the rotary assembly 2 can rotate synchronously; The driven wheel 23 of the turntable 22 can use the power wheel 11 and the link 24 to make the number of accommodating members 3 rotate relative to the two turntables 22, so that the accommodating member 3 assembled in the driven wheel 23 can not only In addition to the synchronous rotation of the rotating assembly 2, the driven wheel 23 can drive the housing member 3 to rotate independently, so as to increase the turnover frequency of the housing member 3 at a stable speed, so that the magnetic beads 41 can be rotated independently. The microtube 4 is repeatedly impacted to achieve a complete and homogeneous effect.

To sum up, the main feature of the biomedical homogenization device of the present invention is that, by virtue of the common connection configuration of the power wheel 11 and the driven wheel 23, the rotating assembly 2 can easily drive the containing member 3 to rotate synchronously and independently , to increase the turnover frequency of the housing member 3 at a stable rotational speed, thereby increasing the number of repeated collisions of the magnetic beads 41 in the microtube 4, so that the microtube 4 can be fully impacted and homogeneous Homogenized substances can achieve the effect of improving the homogenization of substances. Even, when the biomedical homogenization device of the present invention is operating at a medium-high speed, it can still maintain a better homogenization effect by increasing the turnover frequency of the accommodating member 3; As in the case where the traditional magnetic beads 41 fall on the bottom of the microtube 4, the biomedical homogenization device of the present invention is not limited by the speed of the centrifugation speed, but also maintains the effect of stable homogenization.

The biomedical homogenization device of the present invention can increase the repeated impact frequency of the magnetic beads in the microtube at a fixed rotational speed, so as to achieve the effect of improving the homogenization of substances.

The biomedical homogenization device of the present invention can comprehensively impact the substance to be homogenized in the microtube, so as to achieve the effect of complete homogenization of the substance and achieve the effect of maintaining better quality in subsequent experiments.

The biomedical homogenization device of the present invention can complete the homogenization operation in a low-temperature environment to improve the homogenization effect, and is widely applicable to various animal tissues, cells or strains, relatively achieving the effect of improving the usability of the device.

Claims (11)

1. a Biomedicine homogenizing device, is characterized in that comprising:

A pedestal, is provided with at least one power wheel, and this power wheel is arranged at the inwall of this pedestal;

A rotary components, be assembled in rotationally this pedestal, this rotary components comprises a main drive shaft and two rotating disks, this main drive shaft and this power wheel mutually group connect, the two ends of this main drive shaft are respectively arranged with this two rotating disks, and have at least a rotating disk to be provided with at least one follow-up pulley, this power wheel drives this follow-up pulley with respect to turntable rotation; And

At least one accommodating member, is arranged between these two rotating disks rotationally, and with this follow-up pulley mutually group connect.

2. Biomedicine homogenizing device as claimed in claim 1, is characterized in that, this pedestal is provided with two groups of power wheels, and these two groups of power wheels are arranged at respectively the corresponding inwall of this pedestal, and respectively organizes power wheel and be jointly formed by stacking by the power wheel of two identical numbers of teeth.

3. Biomedicine homogenizing device as claimed in claim 2, is characterized in that, these two rotating disks are all provided with one group of follow-up pulley, respectively organize follow-up pulley and be made up of the follow-up pulley of two identical numbers of teeth, and each group of follow-up pulley of these two rotating disks is mutual dislocation configuration.

4. Biomedicine homogenizing device as claimed in claim 2 or claim 3, is characterized in that, this two groups of power wheels are run through respectively at the two ends of this main drive shaft, and are provided with a propulsion source in wherein one end of this main drive shaft.

5. the Biomedicine homogenizing device as described in claim 1,2 or 3, it is characterized in that, this follow-up pulley has a rotation section and a group connecting part, this rotation section this turntable rotation relatively, this group connecting part is located at the internal surface of this rotating disk, to be assembled for this accommodating member by this group connecting part, and limit this accommodating member between this two rotating disk.

6. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, this two rotating disk is not provided with this follow-up pulley part and is separately provided with several connecting pieces, and this connecting piece can be with respect to this turntable rotation.

7. Biomedicine homogenizing device as claimed in claim 6, is characterized in that, one end of this accommodating member is mounted on the group connecting part of this follow-up pulley, and the other end of this accommodating member is mounted on the connecting piece of this rotating disk.

8. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, this main drive shaft periphery wall is provided with one in order to the magnetic part of magnetic force to be provided, and this magnetic part is coated on this main drive shaft periphery wall.

9. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, a gas circulation assembly is separately set in this pedestal, in order to control the air themperature of this pedestal inside.

10. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, these two rotating disks are sticked in respectively the Access Division of this both ends of main drive shaft, and is fixed by a clasp.

11. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, distinguishes and has several tanks in this accommodating member, these several tanks are sealed with a cover plate, and this cover board hub is in the periphery of this accommodating member.

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