CN102988262B - Bee venom composition with acne removing function - Google Patents

Abstract

The invention discloses a bee venom composition with anti-acne effect. It includes anti-acne cosmetic base ingredients and also contains bee venom. The invention solves the problems of existing acne-removing cosmetics such as high irritation, skin allergies, traditional Chinese medicine acne-removing cosmetics leaving dark marks, and bad smell. Oil secretion, antibacterial, anti-inflammatory, promote wound healing, inhibit pigmentation, smooth scars, remove acne marks, etc. The bee venom composition with anti-acne effect of the present invention is various in variety, including cream, lotion, water, gel, jelly and mask. It is not easy to relapse, and is suitable for people with various skin types who are troubled by acne, adult acne, scars, and acne marks.

Description

A kind of bee venom composition with anti-acne effect

Technical field:

The invention belongs to the field of cosmetics, and in particular relates to a bee venom composition with acne-removing effects.

Background technique:

With the acceleration of the pace of life, the increase of work and life pressure, coupled with the frequent occurrence of environmental pollution and food safety issues, the incidence of acne is increasing year by year. Acne is a common chronic pilosebaceous gland inflammatory disease. It occurs in areas with strong sebum secretion such as the face, chest and upper back. The course of the disease is long and easy to recur. Severe cases may have pustules, nodules, cysts or scars. If the inflammation is obvious, it will cause pain or tenderness, accompanied by seborrhea, and even an orange peel face. Acne has a very high incidence rate among young men and women. It not only seriously affects the beauty of the face, but also affects people's social and psychological aspects, causing patients to have negative emotions such as lack of self-confidence, embarrassment, loss, anxiety, tension, irritability, and depression. thereby affecting the quality of life.

At present, the cosmetics for treating acne on the market are mostly added with chemical bactericides, hormones, astringents and anti-inflammatory agents, etc. Although they can sterilize or reduce inflammation in a short period of time, they are very irritating to the skin, and long-term use will increase the burden on the skin. , And cause allergic symptoms such as skin redness, suppuration or pain, and because the pathogenic bacteria are very prone to drug resistance, acne will relapse soon, and form scars or pigmentation. Antibiotics and hormones have been banned by the "Hygienic Standards for Cosmetics" (2007 edition) due to their long-term use and large side effects. In addition, traditional Chinese medicine anti-acne cosmetics are not popular with consumers due to their slow onset, long treatment cycle, unpleasant smell and color, and will leave black or red marks after healing. Therefore, it is particularly urgent to develop a more safe, durable and comprehensive anti-acne cosmetic.

Invention content:

The purpose of the present invention is to provide a safer and longer-lasting acne-removing cosmetic for the problems of the existing acne-removing cosmetics, such as high irritation, skin allergies, and traditional Chinese medicine acne-removing cosmetics, which tend to leave dark marks and smell bad. The bee venom composition has clear functional components, stable quality, no toxic and side effects, no skin allergy, no scar or pigmentation after the acne is cured, and it is not easy to recur after the acne is stopped.

The bee venom composition with anti-acne effect of the present invention includes the base component of the anti-acne cosmetics, and is characterized in that it also contains bee venom.

Preferably, the said bee venom is prepared by the following method. The crude bee venom is dissolved in water, then ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 10,000 Da, and the filtrate is freeze-dried to obtain the bee venom.

The bee venom purification method not only simplifies the bee venom purification process and saves costs, but also can effectively enrich and concentrate active substances such as melittin and melittin, which have significant cosmetic and skin care effects, and remove hyaluronidase , phospholipase A ₂ and other substances that are likely to cause skin allergic reactions, inflammation and pain, while ensuring safety and no skin irritation and allergies, the acne-removing effect is significantly enhanced. The purification method solves the problem in the prior art that the active substances with beauty and skin care effects in the bee venom have not been effectively enriched, fills the gap in the application of the bee venom in acne-removing cosmetics, and fully exerts its anti-acne effect.

Preferably, the content of said bee venom accounts for 0.00001%-2% of the total mass of the bee venom composition.

Said bee venom is preferably prepared by the following method: take crude bee venom and add 10-30 times the volume of water to fully dissolve, centrifuge at 5000-10000g for 10-30min, and ultrafilter the supernatant through an ultrafiltration membrane with a molecular weight cut-off of 10000Da Afterwards, the filtrate was freeze-dried to obtain bee venom.

In order to enhance the effects of anti-inflammatory, sun protection, moisturizing, acne marks removal, etc., the bee venom composition with anti-acne effect of the present invention preferably also contains seaweed polysaccharide and pearl powder, and the content of seaweed polysaccharide accounts for 0.01%- 5%, and the content of pearl powder accounts for 0.2%-15% of the total mass of the bee venom composition.

The seaweed polysaccharide refers to sodium alginate, seaweed polysaccharide, wakame polysaccharide, pearl vegetable polysaccharide, agarose cauliflower polysaccharide, Eucheuma polysaccharide, hijiki polysaccharide, laminaria polysaccharide, sea wormwood polysaccharide, spirulina polysaccharide, sargassum polysaccharide, One or a combination of cuprophyll polysaccharides, fucus polysaccharides, carrageenan polysaccharides, eel polysaccharides and chlorella polysaccharides. Commercially available, but also prepared according to existing methods.

The pearl powder in the present invention refers to one or a combination of nano pearl powder, water-soluble pearl powder, and pearl hydrolyzate liposome. Commercially available, but also prepared according to existing methods.

In order to enhance the effects of dredging pores and balancing oil secretion, the bee venom composition with anti-acne effect of the present invention can also preferably contain PCA zinc and caprylyl salicylic acid, and the content of PCA zinc accounts for 0.01% of the total mass of the bee venom composition. %-1.5%, and the content of octanoyl salicylic acid accounts for 0.1%-2% of the total mass of the bee venom composition.

PCA zinc, caprylyl salicylic acid and cosmetic matrix described in the present invention are all commercially available.

The crude bee venom can be wasp crude bee venom and/or bee crude bee venom, which can be collected by yourself with a bee venom collector, or purchased from the market.

The bee venom composition with anti-acne effect of the present invention is prepared by the following method. The bee venom is prepared in powder form or pre-prepared into an aqueous solution with a mass fraction of 10%-50%, which is homogeneous or mixed with other ingredients, According to the type of acne-removing cosmetics prepared, it can be prepared according to the conventional process, and acne-removing cosmetics such as cream, lotion, water, gel, jelly or mask can be prepared.

The bee venom composition with anti-acne effect of the present invention has good stability, uniform coating and good spreadability. The results of animal experiments and human trial experiments prove that the bee venom composition with anti-acne effect of the present invention is safe, has no irritation or photosensitivity to the skin, can effectively prevent and reduce the damage of ultraviolet rays to the skin, and can also sterilize, reduce inflammation, Moisturizes, balances oil secretion, and improves skin self-protection ability.

The bee venom composition with anti-acne effect of the invention can be used as ordinary cosmetics or functional cosmetics with anti-acne effect.

The solution of the present invention is based on the inventor's understanding of acne formation conditions, pathogenesis, prevention and treatment methods, and the beauty and skin care efficacy of functional active substances, combined with the research achievements of modern acne-removing cosmetics and pharmacology, from many functional active substances Screen out bee venom, seaweed polysaccharide, pearl powder, PCA that can significantly accelerate the metabolism of keratinocytes, dredge hair follicles, regulate oil secretion, antibacterial, bactericidal, anti-inflammatory, moisturizing, acne marks and scars, anti-oxidation, and UV damage protection Zinc and caprylyl salicylic acid, through scientific composition, make them play their respective and mutual synergistic effects significantly:

The bee venom in the bee venom composition with anti-acne effect of the present invention contains rich active ingredients such as melittin, melittin, catecholamines, free amino acids, vitamins, trace elements and glycerin, and has small molecular weight and low immunogenicity. Less prone to allergic reactions.

Melittin can effectively inhibit the proliferation of bacteria and fungi such as Propionibacterium acnes, Escherichia coli, Pseudomonas aeruginosa, Streptococcus and Staphylococcus aureus, reduce the skin's inflammatory response to acne, and have an analgesic effect. It also has significant anti-oxidation, anti-ultraviolet radiation and other effects. Melittin can improve microcirculation, promote the synthesis of elastic fibers and collagen in the dermis, restore skin tension and elasticity, and promote the rapid closure of acne, repair existing dark scars or prevent dark scar formation. Catecholamines have an anti-inflammatory effect on damaged skin caused by acne, light, etc., and can reduce the formation of acne scars. Active substances such as free amino acids, vitamins and trace elements can provide nutrition to the skin, effectively prevent the formation of pigmentation caused by ultraviolet rays on acne, improve keratin metabolism, prevent hyperkeratosis of hair follicles, enhance epidermal barrier function, reduce water loss, It can reduce the damage of air pollutants to the skin, regulate the permeability of the skin, and has the functions of anti-oxidation and promoting skin self-repair. Glycerin can keep the skin hydrated to reduce the secretion of sebaceous glands, and promote the penetration of other active ingredients into the deep layer of the skin, helping to improve the skin's self-repair ability.

The seaweed polysaccharide in the bee venom composition with anti-acne effect of the present invention has extremely strong moisturizing properties, can soften cutin, and has a broad-spectrum bactericidal effect by dissolving the bacterial inner membrane, and can also remove free radicals and prevent skin fat It can prevent skin peroxidation, resist ultraviolet rays, repair damaged cells, and reduce pigmentation. Seaweed polysaccharides can also accelerate the transdermal absorption of acne-removing active ingredients, thereby improving the effect of acne-removing.

The pearl powder in the bee venom composition with anti-acne effect of the present invention contains amino acids such as glycine and methionine necessary for the human body to promote the regeneration of skin collagen, and trace elements such as Zn, which are easily absorbed by the human body and have the functions of sterilization, anti-inflammation, Detoxification, remove acne marks and other effects. Pearl powder can also promote epidermal cell regeneration and wound healing, so that impurities such as dust, oil stains, bacteria, peeling, and dead cells blocked in the skin pores can be discharged smoothly, making the skin delicate, smooth, white and moist. have significant synergies.

The PCA zinc in the bee venom composition with anti-acne effect of the present invention is to replace the sodium ion with zinc ion with antibacterial effect, which can effectively inhibit Propionibacterium acnes, Escherichia coli, Pseudomonas aeruginosa, Streptococcus and gold Staphylococcus aureus and other bacteria and fungi proliferate, and has excellent moisturizing effect. Zinc can regulate the secretion of sebaceous glands and prevent hyperkeratosis of hair follicles by inhibiting 5-α reductase, so as to maintain the oil-water balance of the skin. DNA synthesis, protein synthesis, cell division and maintenance of various enzyme activities in human tissues are inseparable from Zn. Therefore, supplementing Zn to the skin can help maintain the normal metabolism of the skin, promote epidermal cell growth and wound healing, and reduce Skin allergies.

Caprylyl salicylic acid in the bee venom composition with anti-acne effect of the present invention has fat-soluble properties and a relatively large molecular weight, and can penetrate into the stratum corneum and deep pores through the sebaceous glands that secrete oil in the form of fusion with lipids, Dissolves horn plugs, decomposes keratin and dead skin clogged in sebaceous glands and pores, improves pore blockage, blocks acne formation, reduces or inhibits inflammation symptoms, removes blackheads in pores, and shrinks enlarged pores. It can lock the effect on the stratum corneum without affecting the active epidermal cells and dermal tissue. It has good stability, low irritation to the skin, and the probability of cumulative irritation and inflammation is lower than that of ordinary fruit acids. Salicylic Acid also stimulates cell renewal and wound healing to improve skin tone, eliminate blemishes and reveal clear skin.

Compared with the prior art, the present invention has the following beneficial effects:

1. In view of the special physiological structure of the skin and its sensitivity to the ingredients used in cosmetics, the bee venom composition with anti-acne effect in the present invention selects pure natural bee venom as the main raw material. Although it is very rare to add bee venom to cosmetics, bee venom has already been included in the "International Cosmetic Raw Material Standard Chinese Name Catalog (2010 Edition)", which shows that it is safe and feasible to apply bee venom to cosmetics. In addition, in the bee venom composition with anti-acne effect of the present invention, the used bee venom is purified to effectively remove the substances in the crude bee venom that are likely to cause skin allergies, inflammation and pain, and the melittin , Apimin and other ingredients are highly enriched, thus exerting a more significant effect on acne. Simultaneously, the bee venom composition with acne-removing effect of the present invention can be quickly absorbed by the skin without causing skin allergies or bringing any burden to the skin.

2. The bee venom composition with anti-acne effect of the present invention balances oil secretion, dissolves horn plugs, inhibits bacterial proliferation, repairs scars, resists ultraviolet rays, reduces inflammation, resists allergies, resists oxidation, moisturizes, resists post-inflammatory pigmentation, and promotes acne removal. Acne ingredients are absorbed through the skin and promote the healing of acne wounds, etc., and play a comprehensive and significant anti-acne effect, making the skin return to normal, delicate and smooth. After discontinuation, acne and other symptoms are not easy to recur, and the acne-removing effect is remarkable, durable and safe.

3. The bee venom composition with anti-acne effect of the present invention uses pure natural bee venom, which has good compatibility with the skin, can be quickly absorbed by the skin, is safe and mild, does not cause irritation, allergies and other reactions, and has no burden. The bee venom composition with anti-acne effect of the present invention can dredge pores, balance oil secretion, and sterilize bacteria through the synergistic effect of bee venom, seaweed polysaccharide, pearl powder, and bee venom, PCA zinc, and caprylyl salicylic acid. , Anti-inflammatory, anti-acne marks, moisturizing, sun protection and other effects, to comprehensively repair and improve the skin's barrier function, and completely eliminate acne. When the bee venom is compounded with seaweed polysaccharide and pearl powder, the bee venom composition with anti-acne effect of the present invention has particularly remarkable curative effect on inflammatory damage, pustular and cystic acne. When the bee venom is compounded with PCA zinc and caprylyl salicylic acid, the bee venom composition with anti-acne effect of the present invention is particularly effective in curing blackheads, whiteheads, punctate, papular and atrophic acne.

4. The bee venom composition with anti-acne effect of the present invention has various varieties, which can meet the needs of people of different genders, ages and skin types who are troubled by acne, adult acne, acne marks or scars.

Detailed ways:

The following examples further illustrate the present invention, but the embodiments of the present invention are not limited to the following examples, and all equivalent changes or modifications made according to the method of the present invention should be regarded as the scope of protection of the present invention.

The raw materials used below are commercially available unless otherwise specified.

Example 1

Example 2

The crude bee venom was collected by a bee venom collector, fully dissolved by adding 10 times the volume of water, centrifuged at 5000g for 30min, the supernatant was ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 10000Da, and the filtrate was freeze-dried to obtain bee venom.

The bee venom composition with anti-acne effect of the present embodiment is composed of the following components according to the total mass percentage of 100%:

Phase A: carbomer 0.2%, glycerin 4%, propylene glycol 3%, deionized water balance.

Phase B: Cetearyl Ether - 212%, Jojoba Oil 3%, Cetearyl Alcohol 2%, Glyceryl Monostearate 1%, Caprylic/Capric Triglyceride 4%, Ethyl Palmitate 3% hexyl ester, 2% polydimethylsiloxane, 3% isopropyl myristate, 0.1% ethyl paraben.

Phase C: bee venom 0.00001% (added in powder form).

Phase D: Azone/PEG-40 hydrogenated castor oil 1%, triethanolamine 0.2%, phenoxyethanol/methylparaben/butylparaben/ethylparaben/propylparaben/isobutylparaben 0.1 %.

Process: Heat phase A and phase B to 80°C respectively, and add phase B to phase A while stirring. Stir and cool to 45°C, add phase C to the mixture of phases A and B, stir and mix for 10 minutes, then add phase D, mix homogeneously to obtain the bee venom composition with anti-acne effect of this embodiment—containing bee venom acne cream.

The cream prepared in this embodiment has luster, delicate texture, uniform distribution, and no separation of oil and water phases.

Example 3

Purchase the crude wasp venom from Wuhan Yimin Bee Products Co., Ltd., add 30 times the volume of water to fully dissolve it, centrifuge at 10,000 g for 10 minutes, and ultrafilter the supernatant through an ultrafiltration membrane with a molecular weight cut-off of 10,000 Da, then freeze-dry the filtrate to obtain Vespa venom poison.

Use a bee venom collector to collect crude bee venom, add 30 times the volume of water to fully dissolve it, centrifuge at 10,000 g for 10 min, and ultrafilter the supernatant through an ultrafiltration membrane with a molecular weight cut-off of 10,000 Da, then freeze-dry the filtrate to obtain bee venom.

The bee venom composition with anti-acne effect of the present embodiment is composed of the following components according to the total mass percentage of 100%:

Phase A: 5% glycerin, 3% propylene glycol, 2% butanediol, and the balance of deionized water.

Phase B: wasp venom 1%, bee venom 1% (bee venom is added in the form of powder), deionized water 38%.

Phase C: Azone/PEG-40 hydrogenated castor oil 1%, triethanolamine 0.2%, phenoxyethanol/methylparaben/butylparaben/ethylparaben/propylparaben/isobutylparaben 0.1 %.

Process: at 30°C, mix phase A first, then add the pre-mixed phase B into phase A, stir and mix evenly, then add the components of phase C, and mix well to obtain the bee venom combination with acne-removing effect of this embodiment Drug-acne lotion containing bee venom.

The water preparation prepared in this embodiment has no precipitation, high transparency, easy absorption and no stimulation.

Example 4

Purchase honeybee crude bee venom from Fujian Shenfeng Technology Development Co., Ltd., add 15 times the volume of water to fully dissolve, centrifuge at 6000g for 20min, and ultrafilter the supernatant through an ultrafiltration membrane with a molecular weight cut-off of 10000Da, then freeze-dry the filtrate to obtain bee venom .

The bee venom composition with anti-acne effect of the present embodiment is composed of the following components according to the total mass percentage of 100%:

Phase A: xanthan gum 0.4%, acrylate/C _10-30 alkyl acrylate crosspolymer 0.6%, EDTA-2Na 0.1%, glycerin 5%, propylene glycol 5%, butanediol 5%, deionized water balance.

Phase B: 0.5% bee venom (added in the form of a 10% bee venom solution prepared in advance with distilled water), 0.01% laminarin, 3% pearl hydrolyzate liposome, and 20% deionized water.

Phase C: PEG-40 hydrogenated castor oil ester 1%, triethanolamine 0.2%, fragrance 0.1%, phenoxyethanol/methylparaben/butylparaben/ethylparaben/propylparaben/isobutylparaben Esters 0.1%.

Process: Mix the components of phase A and heat to 80°C to stir evenly. When cooled to 40°C, add the pre-mixed evenly phase B, stir for 10 minutes until transparent, add phase C, stir and mix well to obtain the anti-acne product of this example Effective Bee Venom Composition Contains - Acne gel with bee venom.

The gel prepared in this embodiment is transparent, has a fine texture, no agglomeration, and no paste separation under the conditions of -10°C and 40°C.

Example 5

Use a bee venom collector to collect crude wasp venom, add 25 times the volume of water to fully dissolve it, centrifuge at 8000 g for 15 minutes, ultrafilter the supernatant through an ultrafiltration membrane with a molecular weight cut-off of 10000 Da, and freeze-dry the filtrate to obtain wasp venom.

The bee venom composition with anti-acne effect of the present embodiment is composed of the following components according to the total mass percentage of 100%:

Phase A: xanthan gum 0.2%, acrylate/C _10-30 alkyl acrylate crosspolymer 0.6%, glycerin 5%, propylene glycol 3%, deionized water balance.

Phase B: bee venom 0.00001% (add in the form of a 10% bee venom solution pre-prepared with deionized water), sodium alginate 1%, laver polysaccharide 0.5%, wakame polysaccharide 0.5%, pearl vegetable polysaccharide 0.5%, 0.5% polysaccharide from Geliflower, 0.5% polysaccharide from Eucheuma polysaccharide, 0.5% polysaccharide from hijiki, 0.5% polysaccharide from Artemisia chinensis, 0.5% polysaccharide from Sargassum algae, 0.2% water-soluble pearl powder, 30% deionized water.

Phase C: Azone/PEG-40 hydrogenated castor oil 1%, triethanolamine 0.3%, phenoxyethanol/methylparaben/butylparaben/ethylparaben/propylparaben/isobutylparaben 0.1 %.

Process: Mix the components of phase A and heat to 80°C to stir evenly. When cooled to 45°C, add the pre-mixed phase B and stir for 3 minutes. Add the components of phase C in sequence at room temperature and stir evenly to obtain this product The bee venom composition with anti-acne effect of the embodiment—acne-removing gel containing bee venom.

The gel formulated in this embodiment has a light texture and a cooling feel.

Example 6

According to the method for preparing bee venom in Example 2, honeybee venom was obtained.

The bee venom composition with anti-acne effect of the present embodiment is composed of the following components according to the total mass percentage of 100%:

Bee venom 2% (added in the form of powder), spirulina polysaccharide 0.2%, copper algae polysaccharide 0.2%, fucus polysaccharide 0.2%, carrageenan polysaccharide 0.2%, eel polysaccharide 0.2%, chlorella polysaccharide 0.2 %, nano pearl powder 5%, water-soluble pearl powder 10%, almond powder 11%, honey 5%, preservative 1%, deionized water balance. Dissolve bee venom, spirulina polysaccharide, copper algae polysaccharide, fucus polysaccharide, carrageenan polysaccharide, eel polysaccharide, chlorella polysaccharide, and water-soluble pearl powder in deionized water, and dissolve them all at room temperature. Add pre-mixed nano-pearl powder and almond powder, stir and mix at 50r/m for 5 minutes, then add honey and preservatives, stir and mix for 5 minutes to obtain the bee venom composition with anti-acne effect of this embodiment—containing Bee Venom Acne Mask.

Example 7

According to the method for preparing bee venom in Example 3, wasp venom was obtained.

The bee venom composition with anti-acne effect of the present embodiment is composed of the following components according to the total mass percentage of 100%:

Phase A: carbomer 0.1%, glycerin 5%, propylene glycol 3%, deionized water balance.

Phase B: Caprylyl Salicylic Acid 1%, Ceteareth-21.2%, Cetearyl Ether-211.5%, Hydrogenated Polyisobutene 3%, Caprylic/Capric Triglyceride 3%, Palmitic Acid Ethylhexyl 3%, polydimethylsiloxane 2%, ethylparaben 0.1%.

Phase C: 0.5% bee venom (added in the form of a 50% bee venom solution prepared in advance with deionized water), 0.5% PCA zinc, and 20% deionized water.

Phase D: Azone/PEG-40 hydrogenated castor oil 1%, triethanolamine 0.1%, phenoxyethanol/methylparaben/butylparaben/ethylparaben/propylparaben/isobutylparaben 0.1 %.

Process: Heat phase A and phase B to 80°C respectively, slowly add phase B into phase A while stirring. Stir and cool to 55°C, add phase C to the mixture of phases A and B, stir and mix for 10 minutes, then add phase D, mix and homogenize to obtain the bee venom composition with anti-acne effect of this embodiment—containing bee venom Acne Lotion.

The emulsion prepared in this embodiment has a delicate hand feeling, uniform coating, no separation of oil and water, and easy absorption.

Example 8

Use a bee venom collector to collect crude wasp venom, add 25 times the volume of water to fully dissolve it, centrifuge at 8000 g for 15 minutes, ultrafilter the supernatant through an ultrafiltration membrane with a molecular weight cut-off of 10000 Da, and freeze-dry the filtrate to obtain wasp venom.

The bee venom composition with anti-acne effect of the present embodiment is composed of the following components according to the total mass percentage of 100%:

Phase A: 5% glycerin, 3% propylene glycol, 2% butanediol, and the balance of deionized water.

Phase B: bee venom 0.00001% (bee venom is added in the form of powder), PCA zinc 1.5%, deionized water 30%.

Phase C: caprylyl salicylic acid 2%, azone/PEG-40 hydrogenated castor oil 1%, triethanolamine 0.2%, phenoxyethanol/methylparaben/butylparaben/ethylparaben/propylparaben Ester/Isobutylparaben 0.1%.

Process: at 35°C, mix phase A first, then add the pre-mixed phase B to phase A, stir and mix evenly, then add the components of phase C, and mix well to obtain the bee venom combination with acne-removing effect of this embodiment Drug-acne lotion containing bee venom.

The water preparation prepared in this embodiment has no precipitation, high transparency, easy absorption and no stimulation.

Example 9

According to the method for preparing bee venom in Example 4, honeybee venom was obtained.

The bee venom composition with anti-acne effect of the present embodiment is composed of the following components according to the total mass percentage of 100%:

Phase A: xanthan gum 0.4%, acrylate/C _10-30 alkyl acrylate crosspolymer 0.6%, EDTA-2Na 0.1%, glycerin 5%, propylene glycol 5%, butanediol 5%, deionized water balance.

Phase B: 2% bee venom (added in the form of a 10% bee venom solution prepared in advance with distilled water), 0.01% PCA zinc, and 20% deionized water.

Phase C: caprylyl salicylic acid 0.1%, PEG-40 hydrogenated castor oil ester 1%, triethanolamine 0.2%, fragrance 0.1%, phenoxyethanol/methylparaben/butylparaben/ethylparaben/hydroxybenzoate Propyl Phenyl/Isobutyl Paraben 0.1%.

Process: Mix the components of phase A and heat to 80°C to stir evenly. When cooled to 40°C, add the pre-mixed evenly phase B, stir for 10 minutes until transparent, add phase C, stir and mix well to obtain the anti-acne product of this example Powerful Bee Venom Composition - Acne gel with bee venom.

The gel prepared in this embodiment is transparent, has fine texture, no agglomeration, and no paste separation under the conditions of -10°C and 40°C.

Example 10

The following various samples were subjected to animal tests:

The percentages in this embodiment refer to mass percentages.

The wasp venom and honeybee venom of this example were prepared according to the preparation method in Example 3.

Physiological saline (0.9% NaCl solution);

Composition containing crude mee venom (i.e. sample A, crude mee venom content is 2% by mass fraction):

A1: Vespa crude venom 2%, normal saline 98%;

A2: 0.5% crude wasp venom, 1.5% crude bee venom, 98% normal saline.

Composition containing bee venom (i.e. sample B, the content of bee venom is 2% by mass fraction):

B1: Vespa venom 2%, normal saline 98%;

B2: Wasp venom 0.5%, bee venom 1.5%, normal saline 98%.

Composition containing seaweed polysaccharide and pearl powder (i.e. sample C, the total content of seaweed polysaccharide and pearl powder is 2% by mass fraction):

C1: Laminarin 1%, nano pearl powder 1%, saline 98%;

C2: Hijiki polysaccharide 0.5%, sodium alginate 0.5%, water-soluble pearl powder 1%, physiological saline 98%;

C3: 0.5% spirulina polysaccharide, 0.5% eel polysaccharide, 0.5% chlorella polysaccharide, 0.5% water-soluble pearl powder, 98% normal saline;

C4: laver polysaccharide 0.5%, pearl vegetable polysaccharide 0.5%, sargassum polysaccharide 0.5%, pearl hydrolyzate liposome 0.5%, normal saline 98%.

Composition containing bee venom, seaweed polysaccharide and pearl powder (i.e. sample D, the total content of bee venom, seaweed polysaccharide and pearl powder is 2% by mass fraction):

D1: Vespa venom 0.5%, laminarin 0.5%, nano pearl powder 1%, normal saline 98%;

D2: Bee venom 0.3%, Vespa venom 0.2%, Hijiki polysaccharide 0.3%, Sodium alginate 0.2%, Water-soluble pearl powder 1%, Normal saline 98%;

D3: bee bee venom 0.1%, spirulina polysaccharide 0.4%, eel polysaccharide 0.5%, chlorella polysaccharide 0.5%, water-soluble pearl powder 0.5%, normal saline 98%;

D4: Vespa venom 1%, laver polysaccharide 0.2%, pearl vegetable polysaccharide 0.2%, sargassum polysaccharide 0.1%, pearl hydrolyzate liposome 0.5%, normal saline 98%.

Composition containing PCA zinc and octanoyl salicylic acid (i.e. sample E, the total content of PCA zinc and octanoyl salicylic acid is 2% by mass):

E1: PCA zinc 1%, caprylyl salicylic acid 1%, normal saline 98%;

E2: PCA Zinc 1.5%, Caprylyl Salicylic Acid 0.5%, Normal Saline 98%.

Composition containing bee venom, PCA zinc and capryl salicylic acid (i.e. sample F, the total content of bee venom, PCA zinc and capryl salicylic acid is 2% by mass):

F1: Vespa venom 0.5%, PCA zinc 1%, caprylyl salicylic acid 0.5%, normal saline 98%;

F2: bee venom 0.4%, wasp venom 0.4%, PCA zinc 1%, caprylyl salicylic acid 0.2%, normal saline 98%.

Take 102 rabbits, smear thick coal tar solution on the opening of the ear canal on the inner side of each rabbit’s ears once a day, the smearing area is 3cm×3cm, 0.4mL each time, for 14 days continuously, to make rabbit ear acne model, and were randomly divided into 17 groups, namely the normal saline group, the composition group containing crude bee venom (sample A1-A2), the composition group containing bee venom (sample B1-B2), the composition group containing seaweed polysaccharide and pearl powder (sample C1-C4), composition group containing bee venom, seaweed polysaccharide and pearl powder (sample D1-D4), composition group containing PCA zinc and octanoyl salicylic acid (sample E1-E2), containing bee venom, PCA Composition groups of zinc and octanoyl salicylic acid (samples F1-F2), 6 rats in each group.

Apply corresponding samples to the affected parts of both ears of rabbits in each group, about 0.5 mL each time, 3 times/d, for 7 consecutive days. The therapeutic effect was observed daily, and after 7 days and 24 hours after the last drug application, auricular skin biopsy was taken from the same side ear where the drug was applied, and histopathological changes were observed. The pathological changes of the specimens were divided into 4 grades according to the thickness of the epidermis, the expansion degree of the hair follicle opening and the amount of keratin: "0" means no acne; "+" means a small amount of dense keratin in the infundibulum of the hair follicle; A moderate amount of keratinization can be seen in the infundibulum of the hair follicle, which extends to the sebaceous gland; "+++" means that there is extensive keratinization in the expanded hair follicle. Effective rate=(Number of cases at "0" level+Number of cases at "+" level)/Number of experimental cases×100%. The experimental results are shown in Table 1:

Table 1: Effect of samples on the rabbit ear acne model

After 7 days of application of normal saline, there was no significant change in acne, the skin was rough, grainy to the touch, embolisms could be seen in the hair follicle opening of rabbit ears, and inflammatory cell infiltration and nodules could be seen around the sebaceous glands. Compared with the normal saline group, the improvement effect of samples A-F on rabbit ear acne is more obvious.

Since the allergen in the crude bee venom has been removed, compared with the crude bee venom-containing composition (ie sample A), the bee venom-containing composition (i.e. sample B) has no obvious irritation and sensitization to the skin, and Because the active ingredients in the bee venom are enriched, the improvement effect of the bee venom-containing composition on rabbit ear acne is more obvious than that of the crude bee venom-containing composition.

Compared with the composition containing bee venom (i.e. sample B), the composition containing seaweed polysaccharide and pearl powder (i.e. sample C), the effect of containing bee venom, seaweed polysaccharide and pearl powder composition (i.e. sample D) on rabbit ear acne The improvement effect is more obvious, which shows that the seaweed polysaccharide, pearl powder and bee venom in the present invention have a significant synergistic effect.

Compositions containing mee venom, zinc PCA and caprylyl salicylic acid (i.e. sample F) The improvement effect on rabbit ear acne is more obvious, which shows that the PCA zinc, octanoyl salicylic acid and bee venom in the present invention have a significant synergistic effect.

In a word, the bee venom composition with anti-acne effect of the present invention has a very significant therapeutic effect on the rabbit ear acne model, and it can show obvious effect after 4 days of administration.

Example 11

The human trial experiment was carried out on the bee venom composition with anti-acne effect prepared in Examples 1-9, and the samples 1-9 were the bee venom composition with anti-acne effect prepared in Examples 1-9 in turn.

A total of 45 volunteers with mild and moderate facial acne vulgaris were selected, including 15 males and 30 females; aged 17-35 years, with an average of 28.3 years; the course of the disease was 4 weeks to 7 years, with an average of 3.5 years, before applying cosmetics For 2 weeks, no glucocorticoids, retinoic acid and antibiotics, no hyperlipidemia and abnormal liver and kidney function, alcoholism is prohibited, and excessive exposure to strong sunlight or ultraviolet rays is prohibited.

45 volunteers were randomly divided into 9 groups, 5 people in each group. After the subject cleans the skin of the affected part with warm water and wipes it dry, apply the corresponding sample once every night, stop using other drugs or cosmetics during use, and determine the curative effect after 4 weeks (the method of using the mask prepared in Example 6 is: wash the face with warm water On the affected area, apply the mask to the skin of the affected area for 10 minutes and then wash off with water, once a night for a total of 4 weeks). Before treatment and at the 4th week of treatment, count the total number of inflammatory lesions (the sum of inflammatory papules and pustules) and the total number of non-inflammatory lesions (the sum of whiteheads and blackheads) on the face of volunteers, and record in detail after use adverse reactions. Criteria for judging: basic recovery: curative effect index ≥ 90%; marked effect: 60% ≤ curative effect index < 90%; progress: 20% ≤ curative effect index < 60%; invalid: curative effect index < 20%. Efficacy index=(total number of skin lesions before treatment-total number of skin lesions after treatment)/total number of skin lesions before treatment×100%. Effective rate = (basic number of cured cases + number of markedly effective cases) / total number of cases × 100%.

After 4 weeks, each of the bee venom compositions with anti-acne effect of embodiment 1-9 has the therapeutic effect on acne as shown in table 2:

Table 2: Therapeutic effect of the bee venom composition with anti-acne effect on acne

The bee venom composition with anti-acne effect of the present invention has good compatibility with the skin, can be quickly absorbed by the skin, is safe and mild, does not cause irritation, allergies and other reactions, does not burden the skin, and can also dissolve horn plugs, Anti-inflammatory, promote rapid wound healing, repair scars, reduce pigmentation. After using the bee venom composition with anti-acne effect of the present invention for 4 weeks, the skin returned to normal, delicate and smooth, and the acne symptoms were not easy to recur after discontinuation, and the anti-acne effect was safe, significant, long-lasting and comprehensive (Table 2).

Among the above-mentioned 45 volunteers with mild and moderate facial acne vulgaris, there were 648 original facial inflammatory lesions and 997 non-inflammatory lesions. The bee venom composition prepared in Example 4-6 (bee venom, seaweed polysaccharide and pearl powder are the main acne-removing ingredients) totally cured 216 facial inflammatory lesions and 275 non-inflammatory lesions; the bee venom prepared in Example 7-9 The poison composition (bee venom, PCA zinc and caprylyl salicylic acid as the main anti-acne ingredients) cured 177 facial inflammatory lesions and 301 non-inflammatory lesions. It can be seen that when bee venom is compounded with seaweed polysaccharide and pearl powder, the anti-inflammatory, sunscreen, moisturizing, and acne-removing effects of the bee venom composition with anti-acne effect of the present invention are significantly enhanced, and it is effective for curing inflammatory damage, pustule, etc. Sexual and cystic acne have obvious effects. When bee venom is compounded with PCA zinc and caprylyl salicylic acid, the bee venom composition with anti-acne effect of the present invention can significantly enhance the effects of dredging pores, balancing oil secretion, etc., and can cure non-inflammatory damage, spot-like, Efficacy is obvious for papular and atrophic acne.

In a word, the bee venom composition with anti-acne effect of the present invention is suitable for people of different genders, ages and skin types who are troubled by problems such as acne, adult acne, acne marks or scars.

The above-mentioned embodiment is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above-mentioned embodiment, and any other changes, modifications, substitutions, combinations, Simplification should be equivalent replacement methods, and all are included in the protection scope of the present invention.

Claims (1)

1. a bee venom composition with anti-acne effect, comprising anti-acne cosmetic base, is characterized in that, its formula is: (1): In terms of mass fraction, it also includes 0.5% of wasp venom, 0.5% of laminarin and 1% of nano pearl powder; Or (2): In terms of mass fraction, it also includes 0.3% bee meevenom, 0.2% wasp bee venom, 0.3% hijiki polysaccharide, 0.2% sodium alginate and 1% water-soluble pearl powder; Or (3): In terms of mass fraction, it also includes 0.1% honey bee venom, 0.4% spirulina polysaccharide, 0.5% eel polysaccharide, 0.5% chlorella polysaccharide and 0.5% water-soluble pearl powder; Or (4): In terms of mass fraction, it also includes 1% of wasp venom, 0.2% of laver polysaccharide, 0.2% of pearl vegetable polysaccharide, 0.1% of sargassum polysaccharide and 0.5% of pearl hydrolyzate liposome; Or (5): In terms of mass fraction, it also includes 0.5% of Vespa venom, 1% of PCA zinc and 0.5% of octanoyl salicylic acid; Or (6): In terms of mass fraction, it also includes 0.4% honey bee venom, 0.4% wasp venom, 1% PCA zinc and 0.2% octanoyl salicylic acid; The wasp venom is prepared by adding 30 times the volume of the wasp crude venom to fully dissolve it, centrifuging at 10,000 g for 10 minutes, ultrafiltering the supernatant through an ultrafiltration membrane with a molecular weight cut-off of 10,000 Da, and freeze-drying the filtrate to obtain the wasp venom; The honeybee venom is prepared by fully dissolving honeybee crude venom with 30 times the volume of water, centrifuging at 10,000 g for 10 minutes, ultrafiltering the supernatant through an ultrafiltration membrane with a molecular weight cut-off of 10,000 Da, and freeze-drying the filtrate to obtain honeybee venom.