CN102925514B - Fructus Mangifera Indicae purposes in Biological cellulose fermentation culture medium - Google Patents
Fructus Mangifera Indicae purposes in Biological cellulose fermentation culture medium Download PDFInfo
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Abstract
The application relates to the application in Biological cellulose fermentation culture medium of the tropical fruit (tree) Fructus Mangifera Indicae, expands the range of application of Fructus Mangifera Indicae, adds its by-product value；Obtain different mouthfeel and the biology cellulose product of perception simultaneously；Obtain higher yield of biological cellulose and better characteristics through the special nitrogen source of interpolation again, add again the range of application of tropical fruit (tree) Fructus Chaenomelis.In general, it is one the most cost-effective biology cellulose production method.
Description
Technical field
The present invention relates to the production method of a kind of biology cellulose.
Background technology
Biology cellulose (biocellulose, also known as Bacterial cellulose, it is called for short BC) typically refer at different conditions, by the general designation of the cellulose of certain Microbe synthesis in acetic acid Pseudomonas (Acetobacter), Agrobacterium (Agrobacterium), rhizobium (Rhizobium) and Sarcina (Sarcina) etc..Compared with native cellulose, it has the advantageous properties such as high-purity, high-crystallinity, high polymerization degree, high retentiveness and high rehydration.It is widely used to every field.The production of biology cellulose, it is common that by microbial inoculants such as acetic acid Pseudomonas in the culture medium rich in nutritional labelings such as nitrogen source and carbon sources, static fermentation 5-10 days at 20-30 DEG C, translucent biology cellulose thin film can be obtained on the surface of liquid culture medium.
Fermenting and producing biology cellulose usually uses Sucus Cocois as the main component of culture medium at present, although it is with low cost, be prone to application, but its source is more limited to, supply is relatively small, along with the application of biological cellulose membrane product constantly expands, demand is the most increasing, and the production scale of expanding day needs the raw material of more suitable for producing；And, need the biology cellulose product providing mechanical strength bigger in some field, and the product using Sucus Cocois to obtain as fermentation medium can not meet its demand.
Summary of the invention
It is an object of the invention to provide the source of more biology cellulose fermentation substrate, the plantation most commonly seen mainly by tropical area and the production scale the biggest tropical fruit (tree) source as fermentation substrate.Both expand the scope of tropical fruit (tree) application, add its earning rate；Biology cellulose fermentation can be supplied again in large quantity required.
The present invention mainly provides Fructus Mangifera Indicae purposes in Biological cellulose fermentation culture medium, contains the mango juice of percentage by weight 50 ~ 90% in described fermentation medium, and described mango juice sugar content is at more than the 10% of percentage by weight.
Fructus Mangifera Indicae is a kind of typical tropical fruit (tree), and its fruits nutrition enriches, and sugar content is up to 12%～20%, and containing protein 5.56%, fat 16.1%, carbohydrate 67.29%, possibly together with abundant vitamin A, vitamin B, vitamin C etc..It is sugary main based on fructose, sucrose, can be utilized by biology cellulose fermenting and producing bacterium completely, so as fermenting carbon source, being that extraordinary carbon source is originated.And vitamin contained in Fructus Mangifera Indicae and other inorganic elementss can also be utilized for biology cellulose fermenting and producing bacteria growing, can preferably improve its fermentation efficiency.For the ease of using, after Fructus Mangifera Indicae is removed the peel by the present invention, mango juice is made in squeezing concentration, as the main component of fermentation medium, after being adjusted pH value, is directly used in fermenting and producing biology cellulose；Further according to the physiological property of different fermentations strain, it can also be carried out suitable allotment, as added appropriate nitrogen source or inorganic elements etc., be used further to fermentation.
The mango juice for fermentation medium of the present invention can prepare in advance in original producton location, then transports to biology cellulose grown place；But it is so higher for movement requirement；Preferably Mango Fruit is transported to biology cellulose grown place, then prepare use.
The mango juice for fermentation medium of the present invention directly can use after being concentrated into certain sugar content on request, the biology cellulose produced can be with light yellow and light Fructus Mangifera Indicae fragrance, simultaneously because Fructus Mangifera Indicae itself is possibly together with the cellulose of high level, can organically combine with the fibre structure of biology cellulose when fermentation, finer and close solid netted fibre structure is provided, the biology cellulose making fermentation obtain has the mouthfeel of more toughness and beautiful perception, also improves the mechanical strength of biology cellulose；Using after mango juice can also be gone depigmentation, impurity, the biology cellulose produced is pure transparent, and mouthfeel is sharp and clear.
Although the inorganic elements containing relatively horn of plenty and sugar in mango juice, but can deposit after fermentation, in the biology cellulose fermentation later stage, the phenomenon that power is not enough, yield of biological cellulose is relatively low.Finding through research, main cause is the nitrogen source deficiency in sweat needed for fermented bacterium.So, the present invention further provides the source, nitrogen source being suitable for needed for biology cellulose fermenting and producing, it is vegetable protein peptone, and described vegetable protein peptone is to add Fructus Chaenomelis extract hydrolysis gained in vegetable protein；Described Fructus Chaenomelis extract, for gathering immature Fructus Caricae serosity, filters gained after going the removal of impurity by the tannin sedimentation method.The preferred soybean protein of described vegetable protein and/or glutelin.Described vegetable protein peptone is to add in vegetable protein after Fructus Chaenomelis extract hydrolyzes and be dried to powder to obtain；Its molecular weight is about 2000.
The present invention use vegetable protein through Fructus Chaenomelis extract hydrolyze after as biology cellulose fermented bacterium nitrogen source originate, its can fully meet fermented bacterium to nitrogen source needed for, improve fermentation efficiency, improve fermentation product production；This tropical fruit (tree) of Fructus Chaenomelis can also be made full use of rich in Papain enzyme viability；Owing to the preparation of described vegetable protein peptone not only have employed vegetal albumen source, additionally use vegetal protease source, make breaks down proteins in peptone in hgher efficiency, various aminoacid and micromolecule polypeptide content are higher, and kind and character are more conducive to used by fermenting and producing biology cellulose, fermentation efficiency and Product yields more can be improved；And be all food source so that the foodsafety of the biology cellulose that fermenting and producing obtains is guaranteed.
On the basis of the studies above, the present invention provides the Biological cellulose fermentation culture medium of a kind of excellence, and it contains the mango juice of percentage by weight 50 ~ 90%, and described mango juice sugar content is at more than the 10% of percentage by weight；Hydrolyze the vegetable protein peptone obtained through Fructus Chaenomelis extract possibly together with vegetable protein, described Fructus Chaenomelis extract, for gathering immature Fructus Caricae serosity, filters gained after going the removal of impurity by the tannin sedimentation method.
The yield of biological cellulose of the fermentation medium that the yield of biological cellulose utilizing the fermentation of above-mentioned fermentation medium to obtain makees main component than Sucus Cocois can improve more than 10%.And the toughness of product and mechanical strength are also improved to some extent.
Above-mentioned fermentation medium, wherein said vegetable protein is soybean protein and/or glutelin.
Above-mentioned fermentation medium, wherein said vegetable protein peptone is to add in vegetable protein after Fructus Chaenomelis extract hydrolyzes and be dried to powder to obtain；Its molecular weight is about 2000.
The pH value of above-mentioned fermentation medium is 5.2-6.5.Citric acid or glacial acetic acid can be used to be adjusted.
The present invention provides the application in Biological cellulose fermentation culture medium of the tropical fruit (tree) Fructus Mangifera Indicae, expands the range of application of Fructus Mangifera Indicae, adds its by-product value；Obtain different mouthfeel and the biology cellulose product of perception simultaneously；Obtain higher yield of biological cellulose and better characteristics through the special nitrogen source of interpolation again, add again the range of application of tropical fruit (tree) Fructus Chaenomelis.In general, it is one the most cost-effective biology cellulose production method.
Detailed description of the invention
Below in conjunction with embodiment, the detailed description of the invention of the present invention is described in further detail.Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
Embodiment 1:
After fresh mango peeling, squeezing concentrates, and prepares the mango juice that sugar content is the 10% of percentage by weight.PH5.8 is regulated with citric acid.The seed liquor of inoculation biology cellulose fermenting and producing strain, such as acetobacter xylinum, static shallow tray fermentation 5 days at 30 DEG C, obtains membranaceous biology cellulose on the top layer of culture medium.Yield of biological cellulose is 6.9g/L.The elastic modelling quantity of wet biological cellulose membrane is 6.78Gpa.Gained biological cellulose membrane is faint yellow, news have light Fructus Mangifera Indicae fragrance.
Embodiment 2:
After fresh mango peeling, squeezing concentrates, and prepares the mango juice that sugar content is the 15% of percentage by weight.Described mango juice active carbon adsorption goes depigmentation, and small molecular weight impurity is removed in ultrafiltration；The aqueous solution of the magnesium sulfate adding the sodium chloride containing 0.5%, the zinc sulfate of 1% and 1% is diluted to sugar content 12%, regulates pH6.0 with citric acid.The seed liquor of inoculation biology cellulose fermenting and producing strain, such as acetobacter xylinum, static shallow tray fermentation 5 days at 30 DEG C, obtains membranaceous biology cellulose on the top layer of culture medium.Yield of biological cellulose is 7.2g/L.The elastic modelling quantity of wet biological cellulose membrane is 4.95Gpa.Gained biological cellulose membrane is clear, colorless.
Embodiment 3:
After fresh mango peeling, squeezing concentrates, and prepares the mango juice that sugar content is the 15% of percentage by weight.
Gather immature Fructus Caricae serosity, filter after going the removal of impurity by the tannin sedimentation method and prepare Fructus Chaenomelis extract.Being added by described Fructus Chaenomelis extract in soybean protein, water at normal temperature solution 1 day, prepare soy peptone, crushed after being dried, prepared molecular weight is the soy peptone of about 2000.
By following percentage by weight consumption preparation fermentation medium:
The mango juice of sugar content 15%: 60%；
Soy peptone: 10%；
PH5.2 is regulated with glacial acetic acid.
Water: surplus.
In the above-mentioned fermentation medium prepared, inoculate the seed liquor of biology cellulose fermenting and producing strain, such as acetobacter xylinum, static shallow tray fermentation 5 days at 30 DEG C, obtain membranaceous biology cellulose on the top layer of culture medium.Yield of biological cellulose is 10.6g/L.The elastic modelling quantity of wet biological cellulose membrane is 7.28Gpa.Gained biological cellulose membrane is faint yellow, news have light Fructus Mangifera Indicae fragrance.
Embodiment 4:
After fresh mango peeling, squeezing concentrates, and prepares the mango juice that sugar content is the 18% of percentage by weight.Described mango juice active carbon adsorption goes depigmentation, and small molecular weight impurity is removed in ultrafiltration.
Gather immature Fructus Caricae serosity, filter after going the removal of impurity by the tannin sedimentation method and prepare Fructus Chaenomelis extract.Being added by described Fructus Chaenomelis extract in glutelin, water at normal temperature solution 1 day, prepare glutelin peptone, crushed after being dried, prepared molecular weight is the glutelin peptone of about 2000.
By following percentage by weight consumption preparation fermentation medium:
The mango juice of sugar content 18%: 60%；
Glutelin peptone: 10%；
PH5.2 is regulated with glacial acetic acid.
Water: surplus.
In the above-mentioned fermentation medium prepared, inoculate the seed liquor of biology cellulose fermenting and producing strain, such as acetobacter xylinum, static shallow tray fermentation 5 days at 30 DEG C, obtain membranaceous biology cellulose on the top layer of culture medium.Yield of biological cellulose is 11.4g/L.The elastic modelling quantity of biology cellulose is 6.49Gpa.Gained biological cellulose membrane is clear, colorless.
Embodiment 5:
After fresh mango peeling, squeezing concentrates, and prepares the mango juice that sugar content is the 12% of percentage by weight.
Gather immature Fructus Caricae serosity, filter after going the removal of impurity by the tannin sedimentation method and prepare Fructus Chaenomelis extract.Being added by described Fructus Chaenomelis extract in soybean protein, water at normal temperature solution 1 day, prepare soy peptone, crushed after being dried, prepared molecular weight is the soy peptone of about 2000.
By following percentage by weight consumption preparation fermentation medium:
The mango juice of sugar content 12%: 90%；
Soy peptone: 5%；
PH6.2 is regulated with glacial acetic acid.
Water: surplus.
In the above-mentioned fermentation medium prepared, inoculate the seed liquor of biology cellulose fermenting and producing strain, such as acetobacter xylinum, static shallow tray fermentation 5 days at 30 DEG C, obtain membranaceous biology cellulose on the top layer of culture medium.Yield of biological cellulose is 13.7g/L.The elastic modelling quantity of biology cellulose is 8.16Gpa.Gained biological cellulose membrane is faint yellow, news have light Fructus Mangifera Indicae fragrance.
Embodiment 6:
After fresh mango peeling, squeezing concentrates, and prepares the mango juice that sugar content is the 20% of percentage by weight.
Gather immature Fructus Caricae serosity, filter after going the removal of impurity by the tannin sedimentation method and prepare Fructus Chaenomelis extract.Being added by described Fructus Chaenomelis extract in soybean protein, water at normal temperature solution 1 day, prepare soy peptone, crushed after being dried, prepared molecular weight is the soy peptone of about 2000.
By following percentage by weight consumption preparation fermentation medium:
The mango juice of sugar content 20%: 60%；
Soy peptone: 10%；
PH6.2 is regulated with glacial acetic acid.
Water: surplus.
In the above-mentioned fermentation medium prepared, inoculate the seed liquor of biology cellulose fermenting and producing strain, such as acetobacter xylinum, static shallow tray fermentation 5 days at 30 DEG C, obtain membranaceous biology cellulose on the top layer of culture medium.Yield of biological cellulose is 15.3g/L.The elastic modelling quantity of biology cellulose is 8.24Gpa.Gained biological cellulose membrane is faint yellow, news have light Fructus Mangifera Indicae fragrance.
Illustrate: the seed liquor of the fermented bacterium that above-described embodiment is used is under equal conditions, the activation of identical bacterial strain obtains.Gained fermentation results data are the meansigma methods of repeatedly parallel test.
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, on the premise of without departing from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (6)
1. mango juice purposes in Biological cellulose fermentation culture medium together with vegetable protein peptone, it is characterized in that: described fermentation medium contains the mango juice of percentage by weight 50 ~ 90%, after described mango juice is fresh mango peeling, squeezing concentrates gained, and sugar content is more than the 10% of percentage by weight；And wherein possibly together with vegetable protein peptone, described vegetable protein peptone is to add in vegetable protein after Fructus Chaenomelis extract hydrolysis is dried to powder to obtain, and its molecular weight is about 2000；Described Fructus Chaenomelis extract, for gathering immature Fructus Caricae serosity, filters gained after going the removal of impurity by the tannin sedimentation method.
Purposes the most according to claim 1, it is characterised in that: described vegetable protein is soybean protein and/or glutelin.
3. a Biological cellulose fermentation culture medium, it is characterised in that: containing the mango juice of percentage by weight 50 ~ 90%, after described mango juice is fresh mango peeling, squeezing concentrates gained, and sugar content is more than the 10% of percentage by weight；Obtain after Fructus Chaenomelis extract hydrolyzes and is dried to powder possibly together with vegetable protein, molecular weight is the vegetable protein peptone of about 2000, and described Fructus Chaenomelis extract, for gathering immature Fructus Caricae serosity, filters gained after going the removal of impurity by the tannin sedimentation method.
Fermentation medium the most according to claim 3, it is characterised in that: described vegetable protein is soybean protein and/or glutelin.
Fermentation medium the most according to claim 3, it is characterised in that: the pH value of described culture medium is 5.2-6.5.
6. the arbitrary described fermentation medium of claim 3-5, it is used for fermenting and producing biology cellulose.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101347152A (en) * | 2008-08-01 | 2009-01-21 | 内蒙古蒙牛乳业（集团）股份有限公司 | Milk and dairy product containing fruit particles and production method thereof |
| CN101671708A (en) * | 2009-10-19 | 2010-03-17 | 华南理工大学 | Method for fermented-producing bacteria cellulose with pineapple peel juice by two-step method |
| CN101805765A (en) * | 2010-04-01 | 2010-08-18 | 东华大学 | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101347152A (en) * | 2008-08-01 | 2009-01-21 | 内蒙古蒙牛乳业（集团）股份有限公司 | Milk and dairy product containing fruit particles and production method thereof |
| CN101671708A (en) * | 2009-10-19 | 2010-03-17 | 华南理工大学 | Method for fermented-producing bacteria cellulose with pineapple peel juice by two-step method |
| CN101805765A (en) * | 2010-04-01 | 2010-08-18 | 东华大学 | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace |
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