CN102908150A - Composite nervous action potential turning curve calibration and detection system - Google Patents

Abstract

The invention relates to a compound nerve action potential tuning curve calibration and detection system, which is characterized in that it includes a computer, a sound card, an acoustic sensor, a preamplifier, electrodes, a high-impedance input stage, a bioelectric amplifier and earplugs; The test platform, the auditory test platform includes a test execution system and a sound card drive system, and the test execution system includes several calibration and detection modules, wherein the system calibration and debugging module is used to calibrate and debug the sensitivity of the sound card and the acoustic sensor; the acoustic sensor position calibration module is used for The acoustic sensor is calibrated in the external auditory canal of the test animal's ear; the electrode position calibration module is used to calibrate whether the recording electrode is placed on the round window membrane of the test animal's ear; the compound nerve action potential threshold curve detection module is used to test the compound nerve action potential The stimulation intensity threshold value; the compound nerve action potential tuning curve detection module detects the compound nerve action potential tuning curve. The invention can be widely used in hearing detection.

Description

A Compound Nerve Action Potential Tuning Curve Calibration and Detection System

technical field

The invention relates to an auditory detection system, in particular to a sound card-based compound nerve action potential tuning curve calibration and detection system.

Background technique

The compound nerve action potential (CAP) is the cochlea is subjected to transient acoustic stimulation, and after a certain latency period, the sum of the discharges of thousands of individual auditory nerves is recorded through the round window membrane in the ear canal. The synchronicity of the activity of each nerve fiber is a basic condition for whether the round window membrane can record CAP. In order to effectively induce a synchronous response of a large number of nerve fibers, the acoustic signal must have better transient characteristics, that is, a shorter rise/fall time. Because the click or tone burst has good transient characteristics, it is often used to induce CAP. The time domain of Click is a very narrow rectangular pulse with a wide spectrum and poor frequency characteristics. Tone burst is the interception of pure tones in a time window. It has certain frequency characteristics and is a transient signal at the same time. It is often used Based on the superimposed recording of auditory evoked potentials, to understand the auditory sensitivity of the auditory system to this frequency, the present invention uses the stimulating sound Tone burst to detect the CAP threshold curve and CAP tuning curve of the test animal at a specific frequency, the CAP tuning curve (CAP TC) and its Q value are used to characterize the frequency-selective properties of compound neural action potentials at characteristic frequencies.

In 1976, Dallos and Cheatham first used the method of compound nerve action potential to mask another short pure tone with a short pure tone, and successfully recorded the compound nerve action potential tuning curve for objectively evaluating the frequency selection characteristics of the test animal cochlea under physiological conditions. In recent years, there have been some patents related to compound nerve action potentials at home and abroad, for example: the patent application number published on April 13, 2009 is US 20090259140A1, and the patent title applied by Craig A. Buchman is "Stimulating the cochlea with sound or mechanical The method and instrument for recording the corresponding evoked potential in the cochlea" consists of a stimulator and an electrophysiological response recorder. The stimulator includes earphones that generate sound signals, a mechanical stimulator that can generate vibration signals in the patient's inner ear, an ossicular chain direct drive system, Bone anchored hearing aids and bone conduction hearing aids, electrophysiological response recorders include intracochlear diagnostic devices that can be placed in the cochlea and can be removed from the cochlea at the end of the experiment instead of a cochlear implant, which can be used for stand-alone diagnostic purposes or in conjunction with the cochlea Combined with intracellular drug delivery to record stimulus-evoked cochlear or auditory nerve responses such as summation potentials, microphonic potentials, and compound nerve action potentials. The electrocochleogram detection in the above-mentioned patent focuses on the evaluation of hearing impairment and the selection of corresponding therapy. Although the compound nerve action potential is mentioned, it does not involve the detection of the compound nerve action potential tuning curve. Another application number published on August 10, 2000 is US006640121B1, a patent titled "Ear Probe for Cochlear Nerve Detection" invented by Fred Telischi, consisting of an integrated unit with an insertion end and a terminal, the integrated unit includes an optical fiber Laser Doppler flowmeter probes are used to measure blood flow and velocity, as well as recording electrodes for electrocochlear graphs. This patent focuses on the design of ear probes. Similar to the previous invention patent, although this invention mentions compound nerve action potentials , but not related to the detection of compound neural action potential tuning curves.

Contents of the invention

In view of the above problems, the object of the present invention is to provide a compound nerve action potential tuning curve calibration and analysis method capable of quantitatively detecting and analyzing the compound nerve action potential and its tuning curve, and having an acoustic sensor position calibration function and an electrode position calibration function. Detection Systems.

To achieve the above object, the present invention adopts the following technical solutions: a compound nerve action potential tuning curve calibration and detection system, characterized in that: it includes a computer, a sound card, an acoustic sensor, a preamplifier, electrodes, a high impedance input stage, a biological electric amplifier and earplugs, the acoustic sensor includes a miniature speaker and a miniature microphone; the computer is connected to the sound card through a corresponding data interface, the input end of the miniature speaker is connected with the sound card, and the output end of the miniature speaker is connected to the sound card through the sound The tube is inserted in the earplug, the input end of the miniature microphone is inserted in the earplug through the sound transmission tube, and the output end of the miniature microphone is connected with the sound card through the preamplifier; the electrode It includes a recording electrode placed on the round window membrane of the test animal, a reference electrode clamped on the skin wound of the test animal's ipsilateral auricle, and a ground electrode inserted on the back skin of the test animal. The recording electrode, reference electrode and ground electrode The output ends are respectively connected to the input ends of the high-impedance input stage, and the output ends of the high-impedance input stage are connected to the sound card through the bioelectric amplifier; an auditory test platform is set in the computer, and the auditory test platform Including a test execution system and a sound card drive system, the test execution system includes a system calibration and debugging module, an acoustic sensor position calibration module, an electrode position calibration module, a compound nerve action potential threshold curve detection module and a compound nerve action potential tuning curve detection module; The system calibration debugging module is used to calibrate and debug the sensitivity of the sound card and the acoustic sensor; the acoustic sensor position calibration module is used to calibrate the position of the acoustic sensor in the external auditory canal of the test animal ear before the test; the electrode position The calibration module is used to calibrate whether the recording electrode is placed on the round window membrane of the test animal's ear before the test; the compound nerve action potential threshold curve detection module is used for testing within the set test frequency range. Lower the stimulation intensity threshold of the compound nerve action potential, and draw the compound nerve action potential threshold curve to judge whether the auditory intensity sensitivity of the test animal is normal; Next, carry out the compound nerve action potential tuning curve detection according to the set suppression criterion, and draw the relation curve between the suppression sound intensity change and the suppression sound frequency, judge whether the auditory frequency sensitivity of test animal is normal; Described sound card driving system is used for Drive the sound card, perform D/A conversion on the signal sent by the test execution system, and send the electrical signal after D/A conversion to the ear of the test animal through the miniature speaker; The A/D conversion is performed on the signal sent back by the bioelectrical amplifier and the signal after the A/D conversion is sent to the corresponding module of the test execution system.

The electrode position calibration module includes a test parameter setting module, a test signal generation module, a test signal stimulation module, a detection signal acquisition module and a detection signal analysis module; the test parameter setting module is used to set the parameters of the stimulating sound, and the test signal The generation module generates corresponding stimulation sounds according to the set stimulation sound parameters, and sends them to the test signal stimulation module, and the test signal stimulation module sends stimulation sounds, and the stimulation sounds are sent to the tympanic membrane of the test animal through the sound card and the micro-speaker Nearby, the electrode collects the voltage signal on the round window membrane of the test animal or near the round window membrane to amplify the electrical signal through the high impedance input stage and the bioelectric amplifier, and the bioelectric amplifier will amplify the electrical signal through the The sound card is sent to the detection signal acquisition module, and the detection signal acquisition module sends the collected signal to the detection signal analysis module, and the detection signal analysis module judges whether the recording electrode is placed on the test signal according to the collected voltage signal waveform. On the animal's round window membrane, the specific process is: calibrate the position of the recording electrode according to the time-domain waveform of the detected voltage signal. When the detected voltage signal waveform follows the waveform of the stimulus sound well, it is considered that the CM waveform is detected. , the position of the recording electrode is appropriate. If the detected voltage signal waveform does not follow the waveform of the stimulation sound well, adjust the position of the recording electrode until it is suitable.

The compound nerve action potential threshold curve detection module includes a start test button, a test parameter setting module, a short pure tone signal generation module, a short pure tone signal stimulation module, a detection signal acquisition module, a detection signal analysis module and a result analysis display module; the test The parameter setting module is used to set the scope of the test frequency, the scope of the short pure tone stimulation intensity and the test frequency step size, the start test button starts, and the test parameter setting module sends each parameter to the short pure tone signal generation module. The short pure tone signal generation module generates the short pure tone and sends it to the described pure tone signal stimulation module, and the described pure tone signal stimulation module sends the short pure tone to be sent near the tympanic membrane of the test animal through the sound card and the miniature speaker, and the electrodes will test The voltage signal on the animal round window membrane is amplified through the high-impedance input stage and the bioelectric amplifier, and the bioelectric amplifier sends the amplified signal to the detection signal acquisition module through the sound card, and the detection signal The acquisition module sends the collected signal to the detection signal analysis module, and the detection signal analysis module extracts the stimulation sound intensity threshold value of the compound nerve action potential according to the test result under each short pure tone stimulation frequency, and sends the stimulation sound The sound intensity threshold is sent to the result analysis and display module, and so on. When all the short pure tone frequency points are tested, the compound nerve action potential threshold curve is drawn and displayed, and the setting of each short tone stimulation frequency in the test frequency range is completed. The test of the stimulus intensity threshold of the compound nerve action potential; wherein, the extraction process of the stimulus intensity threshold under a certain pure tone stimulation frequency is as follows: continuously increase or decrease the intensity of the burst, and obtain the compound under the stimulation of different intensities of the burst The magnitude of the nerve action potential is to find the corresponding stimulation sound intensity value when the compound nerve action potential is just detected, and the stimulation sound intensity value is the stimulation sound intensity threshold of the compound nerve action potential.

The compound nerve action potential tuning curve detection module includes a start test button, a test parameter setting module, a test signal generation module, a test signal stimulation module, a detection signal acquisition module, a detection signal analysis module and a result analysis display module; the test parameter setting The module sets the stimulating sound frequency, the stimulating sound intensity, the range of suppressing sound frequency, the suppressing sound frequency step size, the range of suppressing sound intensity and the suppression criterion according to the test needs, and the stimulating sound frequency is based on the test of the compound nerve action potential threshold curve detection module. Result is set, and described start test button starts, and described test parameter setting module sends each parameter to described test signal generation module, and described test signal generation module generates short pure tone stimulating sound and narrow-band suppression sound respectively, and sends it to The test signal stimulation module, the test signal stimulation module sends out stimulation sound and suppression sound at the same time, the stimulation sound and suppression sound are sent to the vicinity of the test animal's tympanic membrane through the sound card and the micro-speaker, and the electrodes are connected to the round window membrane of the test animal. The voltage signal is amplified through the high-impedance input stage and the bioelectric amplifier, and the bioelectric amplifier sends the amplified signal to the detection signal acquisition module through the sound card, and the detection signal acquisition module collects the collected The signal is sent to the detection signal analysis module, and the detection signal analysis module extracts the compound nerve action potential at each suppression frequency within the suppression sound frequency range and satisfies the set suppression criterion. The specific process is: at a certain suppression frequency, continuously increase or Reduce the intensity of the suppression sound, stop adjusting when the amplitude of the compound nerve action potential reaches the set inhibition criterion, then the point at this time is a point in the compound nerve action potential tuning curve, and send this point to the result analysis and display module and so on to measure the next inhibition frequency point, when all the inhibition frequency points are tested, the result analysis and display module draws and displays the compound nerve action potential tuning curve.

The acoustic sensor position calibration module includes a test parameter setting module, a test signal generation module, a test signal stimulation module, a detection signal acquisition module and a detection signal analysis module, the test parameter setting module is used to set the parameters of the stimulus sound, and the test The signal generation module generates the corresponding stimulation sound according to the setting stimulation sound parameters, and sends it to the test signal stimulation module, and the test signal stimulation module sends the stimulation sound, and the stimulation sound is sent to the ear of the test animal through the sound card and the micro-speaker , the miniature microphone collects the acoustic signal in the ear canal of the test animal and it will be sent to the preamplifier for amplification, and the preamplifier sends the amplified signal to the detection signal acquisition module through the sound card, so The detection signal acquisition module sends the collected signal to the detection signal analysis module, and the detection signal analysis module judges whether the position of the acoustic sensor is appropriate according to the time domain waveform of the recovery signal.

The system calibration debugging module includes a test parameter setting module, a test signal generation module, a test signal stimulation module, a detection signal recording module, an electroacoustic calibration analysis system and an injection syringe, and the electroacoustic calibration analysis system includes a B&K microphone, a Pulse acquisition Card and notebook, described notebook is provided with Pulse collection system; Described test parameter setting module sets stimulus sound parameter, and described test signal generation module generates corresponding stimulus sound according to the stimulus sound parameter of setting, and sends signal to described test signal Stimulation module, the test signal stimulation module sends a digital stimulation signal to the sound card, and the digital stimulation signal is converted by the D/A module of the sound card to generate an analog signal and send it to the micro-speaker and the Pulse acquisition card at the same time, and the Pulse The acquisition card sends the signal to the Pulse acquisition system; the micro speaker sends the stimulation sound to the injection syringe, the micro microphone and the B&K microphone receive the sound signal in the injection syringe at the same time, and the micro microphone The received sound signal is subjected to acoustic-electric conversion and is amplified by the preamplifier and sent to the sound card and the Pulse acquisition card at the same time, the Pulse acquisition card sends the signal to the Pulse acquisition system, and the sound card transmits the signal to the Pulse acquisition system. The A/D module sends the detection signal recording module after conversion, and the B&K microphone sends the received signal to the Pulse acquisition system through the Pulse acquisition card; according to the digital signal sent by the test signal stimulation module, the described The signal recorded by the detection signal recording module and the signal collected by the Pulse acquisition system obtain the A/D and D/A conversion relationship of the sound card, the electrical-acoustic conversion relationship of the micro-speaker and the acoustic-electric conversion relationship of the miniature microphone.

Because the present invention adopts the above technical scheme, it has the following advantages: 1. The present invention is provided with a test execution system in the computer, and the test execution system includes a system calibration and debugging module, an acoustic sensor position calibration module, an electrode position calibration module, and a compound nerve action potential Threshold curve detection module and compound nerve action potential tuning curve detection module, compared with the prior art, the present invention has increased sound card and acoustic sensor sensitivity calibration, acoustic sensor position calibration and electrode position calibration, can be aimed at the test of calibration module before detection Results The calibration and adjustment of the positions of the acoustic sensors and electrodes effectively ensured the accuracy and precision of the detection results. 2. The present invention integrates the detection of the compound nerve action potential threshold curve and the detection of the compound nerve action potential tuning curve in one test execution system, which not only combines the reflection of the auditory system intensity sensitivity with the reflection of the auditory system frequency sensitivity , and the detection results of the compound nerve action potential threshold curve can guide the detection of the compound nerve action potential tuning curve. Provides a more complete and comprehensive detection means. 3. The present invention adopts a multimedia sound card based on an IEEE 1394 interface, and is connected to a computer through the IEEE 1394 interface. Compared with the existing hardware platform for compound nerve action potential detection, the external sound card does not need a bottom driver, and the system is simple in structure and high in portability , and can achieve high sampling accuracy, easier to build and portable. The invention can be widely used in hearing detection.

Description of drawings

Fig. 1 is a schematic diagram of the system structure of the present invention

Fig. 2 is a schematic structural diagram of the test execution system of the present invention

Fig. 3 is the structural block diagram of the system calibration debugging module of the present invention

Fig. 4 is a structural schematic diagram of the acoustic sensor position calibration module of the present invention

Fig. 5 is a structural schematic diagram of the electrode position calibration module of the present invention

Fig. 6 is a structural schematic diagram of the compound nerve action potential threshold curve detection module of the present invention

Fig. 7 is a structural schematic diagram of the compound nerve action potential tuning curve detection module of the present invention

Detailed ways

The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

As shown in Figure 1, the present invention comprises a computer 1 that is loaded with windows system, sound card 2, a miniature loudspeaker 3, a miniature microphone 4, a preamplifier 5, an electrode 6, a high impedance input stage 7, a Bioelectrical amplifier 8 and a soft earplug 9, wherein, micro-speaker 3 and micro-microphone 4 form an acoustic sensor, in order to isolate the sound in the ear canal from external sound, the output end of micro-speaker 3 and the input end of micro-microphone 4 are inserted Be located in the same earplug 9.

Sound card 2 of the present invention adopts the multimedia sound card that can be connected with computer 1 by IEEE1394 interface, such as the present invention adopts the portable multimedia sound card that has 24bit sampling depth, the highest sampling rate 196kHz produced by RME company when detecting, certainly sound card of the present invention 2. Other structural forms and connection methods can also be used, such as a multimedia sound card or a common sound card connected through a USB interface.

The microspeaker 3 of the present invention includes two electro-acoustic transducers 31, 32 that can produce stimulation sound and suppression sound respectively, and the two electro-acoustic transducers 31, 32 are used to convert the electric stimulation signal that sound card 2 produces To form an acoustic stimulation signal, the input ends of the two electro-acoustic transducers 31, 32 are respectively connected to the output end of the sound card 2 through a TRS interface, and the output ends of the two electro-acoustic transducers 31, 32 are respectively inserted through a sound tube In the earplug 9, the micro-speaker 3 sends the acoustic stimulation signal to the vicinity of the tympanic membrane of the test animal through the earplug 9. Miniature loudspeaker 3 can adopt the various products of prior art, adopts the ER2 plug-in type earphone that Etymotic Company produces when such as the present invention detects, and it has the continuous output ability more than 106 dB SPL, 16kHz operating bandwidth and interaural isolation more than 70dB.

The miniature microphone 4 of the present invention includes an acoustic-electric transducer for collecting acoustic signals near the tympanic membrane of the test animal. The input end of the miniature microphone 4 is inserted in the earplug 9 through a transmission sound tube, and the acoustic signal in the test animal ear canal is converted into an analog voltage signal through the acoustic-electric transducer, and the output end of the miniature microphone 4 is connected to the analog voltage signal through a wire. The analog voltage signal is sent to the preamplifier 5 . The miniature microphone 4 can adopt various products of the prior art, such as the ER-10B+ produced by the U.S. Etymotic Company when the present invention detects.

The preamplifier 5 of the present invention is used to amplify the electrical signal output by the miniature microphone 4, and the amplification factor can be adjusted according to actual needs, and the adjustment factor can be selected from 0dB, 20dB and 40dB. The input end of the preamplifier 5 is connected to the output end of the miniature microphone 4, and the output end of the preamplifier 5 is connected to the sound card 2 through a TRS interface.

Electrode 6 of the present invention is used for recording voltage signal, and it comprises a recording electrode that is placed on the test animal's round window membrane, a reference electrode that is sandwiched in the skin wound of the test animal's ipsilateral auricle, and a reference electrode that is inserted on the test animal's back skin the ground electrode. The recording electrode can be a silver ball electrode, the reference electrode can be an alligator clip electrode, and the ground electrode can be an injection needle. The voltage signal difference between the recording electrode and the reference electrode includes the compound nerve action potential and the cochlear microphone potential.

The high-impedance input stage 7 of the present invention is used to increase the input impedance of the detection circuit, which is beneficial for recording bioelectrical signals, such as compound nerve action potentials.

The bioelectric amplifier 8 of the present invention is used for filtering and amplifying the compound nerve action potential signal and the cochlear microphone potential collected by the electrode 6 . The input end of the bioelectrical amplifier 8 is connected to the output end of the high-impedance input stage 7 through a BNC interface. Connect the sound card 2 through a TRS interface. The bioelectric amplifier 8 of the present invention adopts CP511 produced by Grass Company of the United States. This bioelectric amplifier 8 can provide high gain up to 200k, low noise, high precision and adjustable filter passband range, and can meet the collection requirements of CAP weak signals .

As shown in Fig. 1 and Fig. 2, an auditory test platform is set in the computer 1, and the auditory test platform includes a test execution system 9 and an acoustic card driving system 10, and the test execution system 9 includes a system calibration and debugging module 91, an acoustic sensor position Calibration module 92 , an electrode position calibration module 93 , a compound nerve action potential threshold curve detection module 94 and a compound nerve action potential tuning curve detection module 95 . System calibration debugging module 91 is used for calibrating and debugging the sensitivity of sound card 2 and acoustic sensor; Acoustic sensor position calibration module 92 is used for before testing to the position of acoustic sensor in the external auditory canal of test animal ear, thereby guaranteeing that the position of acoustic sensor is suitable Under the circumstances, the accurate compound nerve action potential tuning curve is tested; the electrode position calibration module 93 is used to calibrate whether the recording electrode is placed on the round window membrane of the test animal ear before the test, so as to ensure that the electrode 6 can collect accurate CAP signals; The compound nerve action potential threshold curve detection module 94 is used for testing to set the test frequency range, the stimulation intensity threshold of the compound nerve action potential under the stimulation of each short pure tone frequency, draws the compound nerve action potential threshold curve, and judges the auditory intensity sensitivity of the test animal. Whether the degree is normal; the compound nerve action potential tuning curve detection module 95 under the short pure tone stimulation of the set frequency and intensity, according to the set inhibition criterion, tests the relationship curve between the intensity change of the inhibition sound and the frequency of the inhibition sound, and judges the test animal Whether the auditory frequency sensitivity is normal.

The sound card driving system 10 is used to drive the sound card 2 to carry out D/A conversion on the signal sent by the test execution system, and the electrical signal after the D/A conversion is sent to the ear of the test animal through the micro speaker 3; Perform A/D conversion with the signal sent back by the bioelectrical amplifier 8, and send the A/D converted signal to the corresponding module of the test execution system 9.

公司的电声校准分析系统为实施例进行说明，它包括一B&K麦克风912、一Pulse采集卡913和一笔记本，笔记本内设置一Pulse采集系统914。将微型扬声器3、微型麦克风4与B&K麦克风912置于注射针筒911，且保证微型扬声器3、微型麦克风4、B&K麦克风912与注射针筒911紧密贴合，使注射针筒911成为一密闭腔体，近似于测试过程中耳塞插入到测试动物鼓膜附近，B&K麦克风912所测的声音强度和微型扬声器3产生声音强度相同。As shown in Figure 3, in the foregoing embodiment, the system calibration debugging module 91 is used to obtain the A/D and D/A conversion relationship of the sound card 2, the electro-acoustic conversion relationship of the miniature speaker 3 and the acoustic-electric conversion relationship of the miniature microphone 4, It includes a test parameter setting module, a test signal generation module, a test signal stimulation module, a detection signal recording module, an electroacoustic calibration analysis system and an injection syringe 911 . The electro-acoustic calibration analysis system can adopt various electro-acoustic calibration analysis systems according to needs, and the present invention is based on the Danish

The company's electro-acoustic calibration and analysis system is described as an embodiment. It includes a B&K microphone 912, a Pulse acquisition card 913 and a notebook, and a Pulse acquisition system 914 is set in the notebook. Place the micro-speaker 3, micro-microphone 4 and B&K microphone 912 in the injection syringe 911, and ensure that the micro-speaker 3, micro-microphone 4, B&K microphone 912 and the injection syringe 911 fit closely, so that the injection syringe 911 becomes a closed cavity body, similar to the earplug inserted near the tympanic membrane of the test animal during the test, the sound intensity measured by the B&K microphone 912 is the same as the sound intensity produced by the micro-speaker 3 .

The test parameter setting module is used to set the parameters of the stimulation sound. The test signal generation module generates the corresponding stimulation sound to the test signal stimulation module according to the set stimulation sound parameters. The test signal stimulation module sends the digital stimulation signal to the sound card 2, and the digital stimulation signal passes through the sound card. The D/A module of 2 converts and generates an analog electrical signal and sends it to the microspeaker 3 and the Pulse acquisition card 913 at the same time, and the Pulse acquisition card 913 sends the collected data to the Pulse acquisition system 914; The transducer 31 sends the stimulating sound to the injection syringe 911, the miniature microphone 4 and the B&K microphone 912 simultaneously receive the sound signal in the injection syringe, and the miniature microphone 4 performs acoustic-electric conversion on the received sound signal and sends it to the preamplifier 5 for further processing. To amplify, the preamplifier 5 sends the amplified signal to the sound card 2 and the Pulse acquisition card 913 simultaneously, and the Pulse acquisition card 913 sends the collected signal to the Pulse acquisition system 914; the A/D module of the sound card 2 performs A/D on the signal After the conversion, the detection signal recording module is sent, and the B&K microphone 912 sends the received signal to the Pulse acquisition system 914 through the Pulse acquisition card 913. The tester stimulates the digital signal sent by the module according to the test signal, the signal recorded by the detection signal recording module and the Pulse acquisition system. The signals collected by 914 are calculated to obtain the D/A and A/D conversion relationship of the sound card 2, the electro-acoustic conversion relationship of the miniature speaker 3 and the acoustic-electric conversion relationship of the miniature microphone 4, specifically:

1) When calibrating and debugging the D/A relationship of sound card 2, the tester uses the digital stimulus signal (measurement point ①) sent by the test signal stimulation module and the analog voltage output by the sound card 2 collected by the Pulse acquisition system 914 (measurement point ② ) to obtain the D/A relationship of the sound card;

2) When calibrating and debugging the A/D relationship of the sound card 2, testers use the analog voltage signal (measurement point ③) collected by the Pulse acquisition system 914 and output from the preamplifier 5 and the digital voltage signal (measurement point ③) collected by the detection signal recording module (measurement Point ④) to obtain the A/D relationship of sound card 2;

3) When calibrating and debugging the micro-speaker 3, the tester is based on the relationship between the analog voltage output by the sound card 2 collected by the Pulse acquisition system 914 (measurement point ②) and the sound pressure signal received by the B&K microphone 912 (measurement point ⑤) Obtain the electro-acoustic conversion relationship of the micro-speaker 3;

4) When the miniature microphone 4 is calibrated and debugged, the relationship between the sound pressure signal (measurement point ⑤) input into the miniature microphone 4 collected by the Pulse acquisition system 914 and the analog voltage signal (measurement point ③) output by the preamplifier 5 is obtained The acoustic-electric conversion relationship of the miniature microphone 4.

As shown in FIG. 4 , in the above embodiments, the acoustic sensor position calibration module 92 includes a test parameter setting module 921 , a test signal generation module 922 , a test signal stimulation module 923 , a detection signal acquisition module 924 and a detection signal analysis module 925 . The test parameter setting module 921 is used to set the parameters of the stimulation sound, and the test signal generation module 922 generates the corresponding stimulation sound according to the setting stimulation sound parameters, and sends it to the test signal stimulation module 923, and the test signal stimulation module 923 sends the stimulation sound, stimulating The sound is sent to the test animal's ear through one of the electro-acoustic transducer 31 of the sound card 2 and the miniature loudspeaker 3, that is: the short pure tone of 1kHz, 50dB SPL is sent to the test animal's ear through the sound card 2 and the electro-acoustic transducer 31, Microphone 4 collects the acoustic signal in the ear canal of the test animal and it will be sent to preamplifier 5 for amplification, and preamplifier 5 sends the amplified signal to detection signal acquisition module 924 through sound card 2, and detection signal acquisition module 924 will collect The signal is sent to the detection signal analysis module 925 for analysis. Since the relative positional relationship between the acoustic sensor and the test animal's tympanic membrane will affect the shape of the waveform of the sampling signal (acoustic signal waveform collected by the miniature microphone), the detection signal analysis module 925 judges whether the location of the acoustic sensor is appropriate according to the time domain waveform of the sampling signal , when the amplitude of the time-domain waveform of the echo signal reaches the amplitude of the sound waveform generated by the test signal generating module 922 and the waveforms of the two are similar, it is considered that the position of the acoustic sensor is suitable; otherwise, the position of the acoustic sensor needs to be adjusted until it is suitable.

As shown in Figure 5, in the above-mentioned embodiments, the electrode position calibration module 93 includes a test parameter setting module 931, a test signal generation module 932, a test signal stimulation module 933, a detection signal acquisition module 934 and a detection signal analysis module. Module 935. The test parameter setting module 931 is used to set the parameters of the stimulation sound, the test signal generation module 932 generates the corresponding stimulation sound according to the set stimulation sound parameters, and sends it to the test signal stimulation module 933, and the test signal stimulation module 933 sends the stimulation sound, One of the electric-acoustic transducers 31 of the sound card 2 and the micro-speaker 3 sends the sound signal to the vicinity of the test animal's tympanic membrane, and the electrode 6 collects the voltage signal on or near the round window membrane of the test animal through high The impedance input stage 7 and the bioelectric amplifier 8 amplify the electrical signal, and the bioelectric amplifier 8 transmits the amplified electrical signal to the detection signal acquisition module 934 through the sound card 2, and the detection signal acquisition module 934 sends the collected signal to the detection signal analysis module 935 , the detection signal analysis module 935 determines whether the recording electrode is placed on the round window membrane of the test animal according to the collected voltage signal waveform. Since whether the recording electrode is placed on the round window membrane of the test animal directly determines whether the CAP and CM signals can be collected, and the CM has a good waveform following effect on the stimulus sound, so the detection signal analysis module 935 detects the signal according to the detected voltage signal. The time-domain waveform is used to calibrate the position of the recording electrode. When the detected waveform of the voltage signal follows the waveform of the stimulus well, it is considered that the CM waveform is detected and the position of the recording electrode is appropriate. If the waveform of the detected voltage signal does not follow the stimulus well If the acoustic waveform changes, adjust the position of the recording electrode until it is suitable.

As shown in Figure 6, in above-mentioned each embodiment, compound nerve action potential threshold value curve detection module 94 comprises a start test button, a test parameter setting module 941, a short pure tone signal generation module 942, a short pure tone signal stimulation module 943, A detection signal acquisition module 944 , a detection signal analysis module 945 and a result analysis and display module 946 .

The test parameter setting module 941 is used to set the scope of the test frequency, the short pure tone stimulation intensity range and the test frequency step size, the user starts the test button, and the test parameter setting module 941 sends each parameter to the short pure tone signal generation module 942, and the short pure tone signal generates Module 942 generates the short pure tone stimulation sound and sends it to the short pure tone signal stimulation module 943, and the short pure tone signal stimulation module 943 sends the short pure tone stimulation sound to the test animal through the sound card 2 and one of the electric-acoustic transducers 31 of the miniature speaker Near the tympanic membrane, the electrode 6 amplifies the voltage signal on the round window membrane of the test animal through the high-impedance input stage 7 and the bioelectric amplifier 8, and the bioelectric amplifier sends the signal amplified by 8 to the detection signal acquisition module 944 through the sound card 2, The detection signal acquisition module 944 sends the collected signal to the detection signal analysis module 945, and the detection signal analysis module 945 extracts the stimulation sound intensity threshold value of the compound nerve action potential just detected under each short pure tone stimulation frequency according to the test results, and uses this Stimulus intensity thresholds are sent to the result analysis display module 946, and so on. When all the short pure tone stimulation frequency points are tested, the compound nerve action potential threshold curve is drawn and displayed, thereby completing the setting of each short tone stimulation within the test frequency range. Tests of stimulus intensity thresholds for compound nerve action potentials at frequencies. Among them, the extraction process of the stimulus sound intensity threshold at a certain stimulation frequency is as follows: continuously increase or decrease the intensity of the short pure tone, obtain the amplitude of the compound nerve action potential under the stimulation of different intensities of the short pure tone, and find the compound nerve action potential just detected (For example, the amplitude of the compound nerve action potential is 10uV, which can be selected according to actual needs, and is not limited here). The stimulus sound intensity value is the stimulus sound intensity threshold of the compound nerve action potential.

Composite nerve action potential threshold curve detection module 94 detects and obtains the composite nerve action potential threshold curve, compares the threshold curve with the hearing threshold of a normal-hearing animal, if the two are basically parallel in the same frequency band and the amplitude difference is within a certain range ( For example, 5dB SPL, which can be selected according to actual needs, is not limited here), it is considered that the hearing of the test animal is normal, if the amplitude of the threshold curve is higher or the hearing threshold of the normal hearing animal is different, the hearing of the test animal is considered abnormal , which is to analyze the auditory system of the test animal from the detection results of the auditory intensity sensitivity; in the detection of the compound nerve action potential tuning curve, it is to analyze the auditory system of the test animal from the frequency sensitivity.

As shown in Figure 7, in above-mentioned each embodiment, compound nerve action potential tuning curve detection module 95 comprises a start test button, a test parameter setting module 951, a test signal generation module 952, a test signal stimulation module 953, a detection A signal collection module 954 , a detection signal analysis module 955 and a result analysis and display module 956 .

The test parameter setting module 951 sets the stimulating sound frequency, stimulating sound intensity, suppressing sound frequency range, suppressing sound frequency step size, suppressing sound intensity range and suppression criterion according to the test needs, and the stimulating sound frequency and intensity are detected according to the compound nerve action potential threshold curve detection module 94 test results, the stimulus frequency is the frequency corresponding to the point with a lower stimulus threshold in the compound nerve action potential threshold curve, and the stimulus intensity is equal to the stimulus threshold increased by 10 or 20 or 30dB SPL (can be determined according to actual needs , not specifically limited here), to ensure that the amplitude of the compound nerve action potential induced is higher than 10uV and not too large. If the amplitude of the compound nerve action potential is less than 10uV, it cannot be detected. The intensity of the suppressing sound used is too high; the upper limit of the intensity of the suppressing sound is set to avoid damage to the hearing of the test animal by the excessively high suppressing sound intensity. If the intensity is low, the lower limit of the suppressed sound intensity can be set to the stimulus sound intensity minus 30dB SPL (can be determined according to actual needs, no specific limitation here), to ensure that the suppressed sound intensity changes within the effective range.

Start the test button to start, and the test parameter setting module 951 sends each parameter to the test signal generation module 952, and the test signal generation module 952 generates short pure tone stimulation sound and narrow-band suppression sound respectively, and sends it to the test signal stimulation module 953, and the test signal stimulation The module 953 emits the stimulation sound and the suppression sound simultaneously, and the stimulation sound and the suppression sound are sent to the vicinity of the test animal's eardrum through the sound card 2 and the two electro-acoustic transducers 31 and 32 . The electrode 6 amplifies the voltage signal on the round window membrane of the test animal through the high-impedance input stage 7 and the bioelectric amplifier 8, and the bioelectric amplifier 8 sends the amplified signal to the detection signal acquisition module 954 through the sound card 2, and the detection signal is collected The module 954 sends the collected signal to the detection signal analysis module 955, and the detection signal analysis module 955 extracts the compound nerve action potential at each suppression frequency within the suppression sound frequency range and meets the set suppression criterion, the specific process is: at a certain suppression frequency Next, continuously increase or decrease the intensity of the inhibitory sound, and stop adjusting when the amplitude of the compound nerve action potential reaches the set inhibition criterion, then the point at this time (inhibition frequency point and corresponding inhibitory sound intensity) is the compound nerve action potential tuning A point in the curve, and this point is sent to the result analysis and display module 956; According to the above process, measure the next suppression frequency point by analogy, when all the suppression frequency points have been tested, the result analysis display module 956 draws and displays the compound nerve The action potential tuning curve is the relationship curve between different inhibition frequency points and the corresponding inhibition sound intensity under the set stimulation frequency and stimulation intensity.

In the above embodiment, the extraction of the compound nerve action potential can adopt coherent averaging and matched filtering methods. Before the test, the stimulus sound was repeated 1024 times (as long as it is a multiple of 2, it can be determined according to actual needs, and there is no limit here), and the coherent average of each response was aligned with each stimulus moment as a reference point, and the obtained The result is used as the template of the compound nerve action potential, and the template is made into the impulse response of the matched filter and stored in the corresponding detection signal analysis module; the detection signal analysis module can pass each detected voltage signal through the matched filter to determine whether There is a compound nerve action potential. If there is a compound nerve action potential, find the peak position of the response. When the matched filter is used to filter to 32 or 64 (multiples of 2, it can be determined according to actual needs, there is no limit here) After the compound nerve action potential waveform is aligned with the peak position, the compound nerve action potential can be extracted by superimposing and averaging.

Above-mentioned each embodiment is only for illustrating the present invention, and wherein the structure of each component and connection mode etc. all can be changed to some extent, every equivalent conversion and improvement carried out on the basis of the technical solution of the present invention, all should not be excluded from the present invention. outside the scope of protection of the invention.

Claims (7)

1. a compound nerve action potential tuning curve calibration and detection system is characterized in that: it comprises computer, sound card, acoustic sensor, preamplifier, electrode, high impedance input stage, bioelectrical amplifier and earplug, and described acoustic sensor comprises Miniature speakers and miniature microphones; the computer is connected to the sound card through a corresponding data interface, the input end of the miniature speakers is connected to the sound card, and the output end of the miniature speakers is inserted in the earplug through a sound tube, so The input end of the miniature microphone is inserted in the earplug through the sound transmission tube, and the output end of the miniature microphone is connected with the sound card through the preamplifier; A recording electrode, a reference electrode clamped on the skin wound of the test animal's ipsilateral auricle, and a ground electrode inserted on the back skin of the test animal, the output ends of the recording electrode, the reference electrode and the ground electrode are connected to the high-impedance input stage respectively. The input end of the high impedance input stage is connected, and the output end of the high impedance input stage is connected with the sound card through the bioelectrical amplifier; An auditory test platform is set in the computer, and the auditory test platform includes a test execution system and a sound card drive system, and the test execution system includes a system calibration and debugging module, an acoustic sensor position calibration module, an electrode position calibration module, and a compound nerve action potential threshold Curve detection module and compound nerve action potential tuning curve detection module; the system calibration debugging module is used for calibrating and debugging the sensitivity of the sound card and the acoustic sensor; the acoustic sensor position calibration module is used for the acoustic sensor before the test. The position in the external auditory canal of the test animal ear is calibrated; the electrode position calibration module is used to calibrate whether the recording electrode is placed on the round window membrane of the test animal ear before the test; the compound nerve action potential threshold curve detection module is used for The test sets the test frequency range, the stimulation intensity threshold of the compound nerve action potential under the stimulation of each short pure tone frequency, and draws the compound nerve action potential threshold curve to judge whether the auditory intensity sensitivity of the test animal is normal; the compound nerve action potential The tuning curve detection module detects the compound nerve action potential tuning curve according to the set inhibition criterion under short pure tone stimulation with set frequency and intensity, and draws the relationship curve between the intensity change of the inhibitory sound and the frequency of the inhibitory sound to judge the behavior of the test animal. Whether the hearing frequency sensitivity is normal; The sound card driving system is used to drive the sound card, perform D/A conversion on the signal sent by the test execution system, and send the electrical signal after the D/A conversion to the ear of the test animal through the micro-speaker; at the same time A/D conversion is performed on the signals sent back by the preamplifier and the bio-electrical amplifier respectively, and the A/D converted signals are sent to corresponding modules of the test execution system. 2. a kind of composite nerve action potential tuning curve calibration and detection system as claimed in claim 1, is characterized in that: described electrode position calibration module comprises test parameter setting module, test signal generation module, test signal stimulation module, detection signal Acquisition module and detection signal analysis module; The test parameter setting module is used to set the parameters of stimulus sound, and the test signal generation module generates corresponding stimulus sound according to the stimulus sound parameter set, and sends it to the test signal stimulus module, the test signal stimulation module emits stimulating sound, and the stimulating sound is sent to the vicinity of the test animal's tympanic membrane through the sound card and the micro-speaker, and the voltage signal on the round window membrane of the test animal or near the round window membrane is collected by the electrode through the The high-impedance input stage and the bioelectric amplifier amplify the electrical signal, and the bioelectric amplifier transmits the amplified electrical signal to the detection signal acquisition module through the sound card, and the detection signal acquisition module sends the collected signal to the A detection signal analysis module, the detection signal analysis module judges whether the recording electrode is placed on the round window membrane of the test animal according to the collected voltage signal waveform, the specific process is: according to the time domain waveform of the detected voltage signal, the recorded Calibrate the electrode position. When the detected voltage signal waveform follows the stimulus waveform well, it is considered that the CM waveform is detected and the recording electrode position is appropriate. If the detected voltage signal waveform does not follow the stimulus waveform well, then Adjust the position of the recording electrode until it is suitable. 3. a kind of compound nerve action potential tuning curve calibration and detection system as claimed in claim 1, is characterized in that: described compound nerve action potential threshold value curve detection module comprises start test button, test parameter setting module, short pure tone signal generation Module, short pure tone signal stimulation module, detection signal acquisition module, detection signal analysis module and result analysis display module; The test parameter setting module is used to set the scope of test frequency, the scope and test frequency step size of short pure tone stimulation intensity, so The start test button starts, the test parameter setting module sends each parameter to the short pure tone signal generation module, the short pure tone signal generation module generates short pure tone and sends it to the short pure tone signal stimulation module, the short pure tone signal The pure tone signal stimulation module sends a short pure tone to the vicinity of the test animal's tympanic membrane through the sound card and the micro-speaker, and the electrodes amplify the voltage signal on the test animal's round window membrane through the high impedance input stage and the bioelectrical amplifier. The bioelectric amplifier sends the amplified signal to the detection signal acquisition module through the sound card, and the detection signal acquisition module sends the collected signal to the detection signal analysis module, and the detection signal analysis module according to the test result Extract the stimulation sound intensity threshold value of the compound nerve action potential just detected under each pure tone stimulation frequency, and send the stimulation sound intensity threshold value to the result analysis and display module, and so on, when all the pure tone frequency points are fully tested Finished, draw and display the compound nerve action potential threshold curve, and complete the test of the stimulation sound intensity threshold value of the compound nerve action potential at each short tone stimulation frequency within the test frequency range; among them, the stimulation sound at a certain short pure tone stimulation frequency The extraction process of the intensity threshold is as follows: continuously increase or decrease the intensity of short pure tones, obtain the amplitude of the compound nerve action potential under the stimulation of different intensities of short pure tones, and find the corresponding stimulation sound intensity value when the compound nerve action potential is just detected. The stimulus intensity value is the stimulus intensity threshold of the compound nerve action potential. 4. a kind of compound nerve action potential tuning curve calibration and detection system as claimed in claim 2, is characterized in that: described compound nerve action potential threshold value curve detection module comprises start test button, test parameter setting module, short pure tone signal generation Module, short pure tone signal stimulation module, detection signal acquisition module, detection signal analysis module and result analysis display module; The test parameter setting module is used to set the scope of test frequency, the scope and test frequency step size of short pure tone stimulation intensity, so The start test button starts, the test parameter setting module sends each parameter to the short pure tone signal generation module, the short pure tone signal generation module generates short pure tone and sends it to the short pure tone signal stimulation module, the short pure tone signal The pure tone signal stimulation module sends a short pure tone to the vicinity of the test animal's tympanic membrane through the sound card and the micro-speaker, and the electrodes amplify the voltage signal on the test animal's round window membrane through the high impedance input stage and the bioelectrical amplifier. The bioelectric amplifier sends the amplified signal to the detection signal acquisition module through the sound card, and the detection signal acquisition module sends the collected signal to the detection signal analysis module, and the detection signal analysis module according to the test result Extract the stimulation sound intensity threshold value of the compound nerve action potential just detected under each pure tone stimulation frequency, and send the stimulation sound intensity threshold value to the result analysis and display module, and so on, when all the pure tone frequency points are fully tested Finished, draw and display the compound nerve action potential threshold curve, and complete the test of the stimulation sound intensity threshold value of the compound nerve action potential at each short tone stimulation frequency within the test frequency range; among them, the stimulation sound at a certain short pure tone stimulation frequency The extraction process of the intensity threshold is as follows: continuously increase or decrease the intensity of short pure tones to obtain the amplitude of the compound nerve action potential under the stimulation of different intensities of short pure tones, and find the corresponding stimulation sound intensity value when the compound nerve action potential is just detected. The stimulus intensity value is the stimulus intensity threshold of the compound nerve action potential. 5. a kind of compound nerve action potential tuning curve calibration and detection system as claimed in claim 1 or 2 or 3 or 4, is characterized in that: described compound nerve action potential tuning curve detection module comprises start test button, test parameter setting Module, test signal generation module, test signal stimulation module, detection signal acquisition module, detection signal analysis module and result analysis display module; the test parameter setting module sets the range of stimulation sound frequency, stimulation sound intensity, and suppression sound frequency according to test needs , sound suppression frequency step size, the scope and suppression criterion of sound suppression intensity, the stimulation sound frequency is set according to the test results of the compound nerve action potential threshold curve detection module, the start test button starts, and the test parameter setting module sends Each parameter is sent to the test signal generation module, and the test signal generation module generates short pure tone stimulation sound and narrow-band suppression sound respectively, and sends it to the test signal stimulation module, and the test signal stimulation module sends stimulation sound and Suppressing sound, stimulating sound and suppressing sound are sent to the vicinity of the test animal's tympanic membrane through the sound card and the micro-speaker, and the electrodes amplify the voltage signal on the test animal's round window membrane through the high-impedance input stage and the bioelectrical amplifier, The bioelectric amplifier sends the amplified signal to the detection signal acquisition module through the sound card, and the detection signal acquisition module sends the collected signal to the detection signal analysis module, and the detection signal analysis module extracts the suppressed sound frequency The compound nerve action potential that satisfies the set inhibition criterion at each inhibition frequency within the range, the specific process is: at a certain inhibition frequency, continuously increase or decrease the intensity of the inhibition sound, when the amplitude of the compound nerve action potential reaches the set inhibition criterion When the adjustment is stopped, the point at this time is a point in the compound nerve action potential tuning curve, and this point is sent to the result analysis and display module; and so on to measure the next inhibition frequency point, when all the inhibition frequency points are tested After that, the result analysis and display module draws and displays the compound nerve action potential tuning curve. 6. A compound nerve action potential tuning curve calibration and detection system according to any one of claims 1 to 5, wherein the acoustic sensor position calibration module includes a test parameter setting module, a test signal generation module, a test Signal stimulation module, detection signal acquisition module and detection signal analysis module, the test parameter setting module is used to set the parameters of stimulation sound, and the test signal generation module generates corresponding stimulation sound according to the setting stimulation sound parameters, and sends it to Described test signal stimulation module, described test signal stimulation module sends stimulation sound, and stimulation sound is sent to test animal ear through described sound card and miniature loudspeaker, and described miniature microphone collects the sound signal in the ear canal of test animal and it will send to all The preamplifier is amplified, and the preamplifier sends the amplified signal to the detection signal acquisition module through the sound card, and the detection signal acquisition module sends the collected signal to the detection signal analysis module. The detection signal analysis module judges whether the location of the acoustic sensor is appropriate according to the time domain waveform of the sampling signal. 7. A compound nerve action potential tuning curve calibration and detection system according to any one of claims 1 to 6, characterized in that: the system calibration and debugging module includes a test parameter setting module, a test signal generation module, a test signal Stimulation module, detection signal recording module, electroacoustic calibration analysis system and injection syringe, described electroacoustic calibration analysis system includes B&K microphone, Pulse acquisition card and notebook, and Pulse acquisition system is arranged in the notebook; The test parameter setting module sets the stimulation sound parameters, and the test signal generation module generates corresponding stimulation sounds according to the set stimulation sound parameters, and sends signals to the test signal stimulation module, and the test signal stimulation module sends digital stimulation signals to Described sound card, digital stimulus signal generates analog signal after the D/A module conversion of described sound card is sent to described miniature loudspeaker and Pulse acquisition card simultaneously, and described Pulse acquisition card sends signal to described Pulse acquisition system; The micro-speaker sends the stimulating sound to the injection syringe, the micro-microphone and the B&K microphone simultaneously receive the sound signal in the injection syringe, and the micro-microphone converts the received sound signal into sound and electricity and transmits it through the front After the amplifier is amplified, it is sent to the sound card and the Pulse acquisition card simultaneously, and the Pulse acquisition card sends the signal to the Pulse acquisition system, and the sound card sends the signal to the detection signal recording module after the A/D module conversion, The B&K microphone sends the received signal to the Pulse acquisition system through the Pulse acquisition card; according to the digital signal sent by the test signal stimulation module, the signal recorded by the detection signal recording module and the Pulse acquisition system acquisition The A/D and D/A conversion relationship of the sound card, the electrical-acoustic conversion relationship of the miniature speaker and the acoustic-electric conversion relationship of the miniature microphone are obtained from the signal.

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