CN102860975A - Skin-whitening and moisture-preserving skin-care product for eyes and preparation method for skin-whitening and moisture-preserving skin-care product - Google Patents
Skin-whitening and moisture-preserving skin-care product for eyes and preparation method for skin-whitening and moisture-preserving skin-care product Download PDFInfo
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Abstract
The invention discloses a skin-whitening and moisture-preserving skin care product for eyes and a preparation method for the skin-whitening and moisture-preserving skin-care product. The skin-whitening and moisture-preserving skin-care product contains the following ingredients in part by weight: 30 to 60 parts of alpha-mannosan, 0.01 to 0.5 part of tuckahoe sclerotium extract, 0.1 to 5 parts of angelica polysaccharide, 0.1 to 5 parts of angelica total organic acid, 0.1 to 6 parts of largehead atractylodes rhizome root extract and 40 to 75 parts of inocular mixture. After being used, the skin-whitening and moisture-preserving skin-care product has obvious skin-whitening and moisture-preserving effects, and has high capacity of inhibiting melanin and increasing the brightness of skin.
Description
Technical field
The present invention relates to a kind of eye whitening skin and preserving moisture skin care item and preparation method thereof.
Background technology
Eyelid skin is the thinnest skin of Whole Body, along with the growth of physiological age, and the puzzlement of some external factor such as the stimulation of UV light and disease factor, the linked groups at eyes position and top layer skin, the catabiosis such as the easiest generation black eye, eye pouch and wrinkle.Black eye are that the eyelid outward appearance at eye socket position presents darker color, with the pigment number of eyelid itself, the factor analysis such as vascular flow in the eyelid.The reason that black eye form is a lot, mainly contains that (1) does not have enough sleep, malnutrition, calm with eyestrain, bad life habits, cosmetic colors; (2) hormone of health can change after intermenstrual period, conceived latter stage or the fertility, and pigmentation produces black eye at eye; (3) human lesion such as hypertension, diabetes, kidney disease, hepatic disease, eipathia asthenia person etc. near the eyes the bad double venous return of subcutaneous tissue blood supply also the bad eye QI-blood circulation that causes is not normal, blood stasis retardance and cause black eye.
Eye-care product on the market are more now, are some daily moisture retentions mostly, and its composition mostly is the synthetic nutritional labeling of artificial chemistry, and cost is higher, and produces the untoward reaction such as irritated easily.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of eye moisturizing Skin whitening care cosmetics, contain the composition of following parts by weight:
α-mannan 30-60;
Poria cocos sclerotium extract 0.01-0.5;
Radix Angelicae Sinensis polysaccharide 0.1-5;
Radix Angelicae Sinensis total organic acids 0.1-5;
Rhizoma Atractylodis Macrocephalae root extract 0.1-6;
Eye encloses agent 40-75.
Preferably, eye moisturizing Skin whitening care cosmetics is gel, also contains the composition of following parts by weight:
Deionized water 600-900;
Glycerol 20-45;
Butanediol 30-50;
Xanthan gum 0.1-5;
Carbomer U20 4-8;
Pantothenylol 2-8;
EDTA disodium 0.1-3;
Two-PEG-18 methyl ether dimethylsilane 3-7.
Preferably, above-mentioned eye moisturizing Skin whitening care cosmetics also contains the composition of following parts by weight: stimulation inhibitory factor 10-40.
Preferably, above-mentioned eye moisturizing Skin whitening care cosmetics also contains the composition of following parts by weight: antipruritic and antipruritic agent 10-50.
Preferably, above-mentioned eye moisturizing Skin whitening care cosmetics also contains the composition of following parts by weight: the mixture 0.5-5 of Methylisothiazolinone and any ratio of iodine propilolic alcohol butyl mephenesin Carbamate.
The present invention also provides the preparation method of above-mentioned eye moisturizing Skin whitening care cosmetics, comprises the steps:
(1) consumption glycerol, butanediol disperse yellow virgin rubber add entry and stir, and be for subsequent use;
(2) water intaking adds in the emulsion tank, and carbomer U 20 evenly is sprinkled upon water surface, is dipped to water surface without white powder, and is stand-by;
(3) described amount α-mannan, EDTA disodium and water are added in step (2) the gained mixture through 200 eye mesh screens;
(4) described amount pantothenylol is added to the water heating for dissolving after, add in step (3) the gained mixture through 200 eye mesh screens;
(5) poria cocos sclerotium extract, Radix Angelicae Sinensis polysaccharide, Radix Angelicae Sinensis total organic acids, Rhizoma Atractylodis Macrocephalae root extract and two-PEG-18 methyl ether dimethylsilane are added to the water heating for dissolving fully after, add in step (4) the gained mixture through 200 eye mesh screens;
(6) step (1) gained mixture is added in step (5) the gained mixture through 200 eye mesh screens, the vacuum mix and blend is heated to 80 ~ 85 ℃, insulation, and stirring is cooled to below 45 ℃;
(7) step (6) gained mixture is crossed 200 eye mesh screens, transfers pH to 3.5 ~ 8.5, and then vacuum stirring adds the pleasing to the eye agent that encloses, and vacuum stirring is cooled to 38 ℃, more than the ageing 24h.
Preferably, in the step (3) also with stimulation inhibitory factor behind 200 mesh sieves, add in step (2) the gained mixture.
Preferably, in the step (3) also with antipruritic and antipruritic agent behind 200 mesh sieves, add in step (2) the gained mixture.
Preferably, step (6) gained mixture in the step (7) is crossed 200 eye mesh screens, transfer pH to 3.5 ~ 8.5, then vacuum stirring adds the pleasing to the eye mixture that encloses agent and Methylisothiazolinone and any ratio of iodine propilolic alcohol butyl mephenesin Carbamate, vacuum stirring is cooled to 38 ℃, more than the ageing 24h.
Preferably, step (6) gained mixture in the step (7) is crossed 200 eye mesh screens, transfers pH to 3.5 ~ 8.5 with the NaOH aqueous solution.
Technical scheme provided by the invention can reach following technique effect:
1, rigorous, the exact efficacy of scientific formulation has no side effect.
2, the efficacy study test result shows: the present invention uses rear moisturizing whitening effect remarkable, has preferably check melanin ability and increase skin brightness ability, has simultaneously preferably moistening effect, the stronger ability that moisturizes is arranged and continue lock water effect.
3, human body zest and patch experimental studies results show, the present invention is safe and non-stimulating, and human body is had no adverse reaction.Irritation test shows that the present invention's (institute's test system concentration is 4000 μ g/ml and 8000 μ g/ml) is equal nonirritant under RBC Test System test system.The human body patch is tested all experimenters and is all had no adverse reaction, and illustrates that the present invention has no adverse reaction to human body.
Description of drawings
Fig. 1 is the rate of change that the present invention uses rear dermal melanin content;
Fig. 2 is the relative change rate that the present invention uses rear dermal melanin content;
Fig. 3 is the rate of change that the present invention uses rear skin content of hemachrome;
Fig. 4 is the relative change rate that the present invention uses rear skin content of hemachrome;
Fig. 5 is the situation of change that the present invention uses rear hydration rate;
Fig. 6 is the situation of change that the present invention uses the relative increment rate of rear moisture;
Fig. 7 is the situation of change that the present invention uses rear percentage of water loss;
Fig. 8 is that the present invention uses rear relative percentage of water loss situation of change;
Fig. 9 is that SDS detects the erythrocyte integrity
Figure 10 is the microphotograph of erythrocyte.
The specific embodiment
The invention will be further described below in conjunction with the drawings and specific embodiments, can be implemented so that those skilled in the art can better understand the present invention also, but illustrated embodiment is not as a limitation of the invention.
Eye moisturizing Skin whitening care cosmetics embodiment consumption of the present invention following (weight portion):
The preparation process of above-mentioned gel is:
(1) with glycerol, butanediol in advance the disperse yellow virgin rubber stir, add 100 parts of water and stir, for subsequent use;
(2) taking by weighing 396-684 part water adds in the emulsion tank, with carbomer U 20(acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross linked polymer) evenly be sprinkled upon water surface (trying not to be spread across on pot wall), wait to soak fully (about 10min, the surface is standard without white powder) after, stand-by;
(3) with α-mannan, BioAegis(or do not add), BioCalm(or do not add), EDTA disodium and 4-16 part water adds in step (2) the gained mixture through 200 eye mesh screens;
(4) general alcohol and water adds in step (3) the gained mixture by 200 eye mesh screens by weight the 1:2 heating for dissolving;
(5) poria cocos sclerotium extract, Radix Angelicae Sinensis polysaccharide, Radix Angelicae Sinensis total organic acids, Rhizoma Atractylodis Macrocephalae root extract, two-PEG-18 methyl ether dimethylsilane and 100 parts of water heating for dissolving are added in step (4) the gained mixture by 200 eye mesh screens fully;
(6) step (1) gained mixture is added in step (5) the gained mixture through 200 eye mesh screens, vacuum mix and blend (300r/min) is heated to 80 ~ 85 ℃ of insulation 30min, stirs (300r/min) and cools to below 45 ℃;
(7) step (6) gained mixture is crossed 200 eye mesh screens, adding weight percent concentration 10%NaOH aqueous solution transfers pH to 3.5 to 8.5, vacuum stirring (500r/min) 5min, then add and pleasing to the eyely enclose prewired mixture 338Y324, MTI(or do not add), vacuum stirring (500r/min) 15min, be cooled to 38 ℃, packing behind the ageing 24h.
Deionized water of the present invention is 600～900 parts, and minute four addings add 100 parts of deionized waters in step 1, add deionized water 396-684 part in step 2, add deionized water 4-16 part in step 3, add 100 parts of deionized waters in step 4.
The various extracts such as described poria cocos sclerotium extract, Radix Angelicae Sinensis polysaccharide, Radix Angelicae Sinensis total organic acids, Rhizoma Atractylodis Macrocephalae root extract are commercially available or extract with the conventional method of prior art and to obtain that the present invention is not described further.
Eye encloses agent and is preferably eye and encloses prewired mixture 338Y324 in the embodiments of the invention.
The BioAegis(stimulation inhibitory factor), BioCalm(antipruritic and antipruritic agent) (available from the many fragrant bio tech ltds of Beijing China) can obviously improve the present invention to the zest of skin, reduce irritated, MTI(Methylisothiazolinone/iodine propilolic alcohol butyl mephenesin Carbamate is available from Bristol specialty chemicals (Shanghai) Co., Ltd.) make more stable quality of the present invention.
Good, the pH value of obvious, the sense organ of prepared finished product effect and denseness is moderate, steady quality according to the embodiment of the present invention, the indices of embodiment 5 is best, is best mode for carrying out the invention.
The present invention has preferably moistening effect simultaneously to suppressing and to improve eye black eye effect remarkable, the stronger ability that moisturizes is arranged and continue lock water effect, and is safe and non-stimulating, now relevant result of study is reported as follows:
One, white-skinned face function evaluation
Method:
The experimenter amounts to 32 people.Concrete sex composition is determined at random.If there are during this time 2 people untoward reaction to occur, then stop test; The result does not find untoward reaction.After the experimenter had smeared the eye Skin whitening care cosmetics that the present invention arbitrary embodiment makes, dermal melanin all had obvious reduction.
1. select the left and right eye of experimenter as tested zone.Every experimenter's left eye is smeared given the test agent (the eye Skin whitening care cosmetics that the arbitrary embodiment of the present invention makes) as sample sets, and right eye is not smeared any sample as the blank group.
2. use dermal melanin and haemachrome before cosmetics are smeared at dermal melanin and haemachrome tester determination experiment position by the technical staff.
3. sooner or later in the tested district (the same area) of cleaning through clear water, smear testing product every day, experimental session, the experimenter can not be coated with other any cosmetics outside the tested sample of erasing at the experiment position.
The experimenter make continuously the week that applies some make up, two weeks, three weeks and all around after, same time experimenter will smear the position and clean weekly, use dermal melanin and haemachrome tester to measure the spreader portion bit value by the tester.
5. the statistics experimenter tests the numerical value that the position records at every turn, the Changing Pattern of analyzing skin melanin and haemachrome.
The result:
(1) melanin content mutation analysis
The dermal melanin changes of contents was reflected in test period, Experimental Area dermal melanin content change with time.Its value is larger, and dermal melanin content is larger, otherwise dermal melanin content is less.Melanin content (T0) before each time point melanin content (Tn)-use product behind melanin content difference (Δ T)=use product.
The difference of the tested position of table 1 dermal melanin content and initial value
Within 4 weeks of test period, the melanin content difference of sample sets is negative value always.
(2) data variation of the relative initial value of melanin
Melanin rate of change=(sample sets melanin-sample sets melanin initial value)/sample sets melanin initial value, this value is less, illustrates that the test specimen black eye effect of dispelling is more obvious.
The result: analysis result sees accompanying drawing 1 for details.
As seen from Figure 1, within test period, the rate of change of the melanin content at tested position is reduction trend, and is lower than blank group always; Sample uses 4th week, and the rate of change of sample sets melanin content reaches minimum-5.53%, and there is significant difference (p＜0.05) in initial value relatively.
(3) the melanin relative change rate relatively
Melanin relative reduce rate=(sample sets melanin content-blank group melanin content)/blank group melanin content, this value is less, illustrates that the relative blank sample of the test specimen black eye effect of dispelling is more obvious.
The result: analysis result sees accompanying drawing 2 for details.
As seen from Figure 2, within test period, the relative change rate of the melanin content at tested position is reduction trend, and is negative value always; Sample uses 4th week, and the relative reduce rate of sample sets melanin content reaches minimum-5.79%.
(4) content of hemachrome mutation analysis
The variation of skin content of hemachrome is reflected in test period Experimental Area skin content of hemachrome change with time.Its value is larger, and the skin content of hemachrome is larger, otherwise the skin content of hemachrome is less.Content of hemachrome (T0) before each time point content of hemachrome (Tn)-use product behind content of hemachrome difference (Δ T)=use product.
The difference of the tested position of table 2 skin content of hemachrome and initial value
Within 4 weeks of test period, the content of hemachrome difference of sample sets is negative value always.
(5) data variation of the relative initial value of haemachrome
Haemachrome reduction rate=(sample sets haemachrome-sample sets haemachrome initial value)/sample sets haemachrome initial value, this value is less, illustrates that the test specimen black eye effect of dispelling is more obvious.
The result: analysis result sees accompanying drawing 3 for details.
As seen from Figure 3, within test period, the rate of change of the content of hemachrome at tested position is reduction trend, and is lower than blank group always; Sample uses 4th week, and the rate of change of sample sets content of hemachrome reaches minimum-7.78%.
After sample used for 3 weeks, there was significant difference (p＜0.05) in the relative initial value of skin content of hemachrome.
(6) the haemachrome relative change rate relatively
Haemachrome relative reduce rate=(sample sets content of hemachrome-blank group content of hemachrome)/blank group content of hemachrome, this value is less, illustrates that the relative blank sample of the test specimen black eye effect of dispelling is more obvious.
The result: analysis result sees accompanying drawing 4 for details.
As seen from Figure 4, within test period, the relative change rate of the content of hemachrome at tested position is reduction trend, and is negative value always; After sample used 4th week, the relative reduce rate of sample sets content of hemachrome reached minimum-7.88%.
The result
Show according to above result of the test:
After eye Moisturizer of the present invention is used, within test period, the melanin content at tested position is reduction trend always, and has significant difference (p＜0.05) when the 4th week, its tested position melanin content has the reduction effect, and namely sample has the ability of check melanin calmness.
Within test period, the content of hemachrome at tested position is reduction trend always, and has significant difference (p＜0.05) the 3rd week, and its tested position content of hemachrome has certain reduction effect, and namely sample has the Pigmented ability of inhibition.
Two, moisture-keeping efficacy evaluation
Method:
The experimenter amounts to 30 people, and concrete sex composition and age structure determine that at random within test period, untoward reaction is not found at tested position.
1. select successively cycle labeling of the left and right arm of experimenter: tested region samples group (smearing the eye skin care item that the present invention makes) and blank zone;
2. the technical staff uses moisture content of skin test Corneometer CM825, moisture to measure respectively tested zone and blank zone through the lost test of skin Tewameter TM300, averages, and is designated as initial value.
3. smear submitted sample the experimenter through the experiment position that clear water cleaned, any sample is not smeared in the blank zone
The experimenter make continuously applied some make up one hour, two hours, four hours after, use moisture content of skin test Corneometer CM825, moisture to measure respectively tested zone and blank zone 5 times through the skin test Tewameter TM300 that scatters and disappears by the technical staff, average.
5. add up the numerical value that records, analyze its moisture of skin and moisture loss Changing Pattern.
Interpretation of result:
(1) moisture content change
Moisture content change was reflected in test period, Experimental Area moisture change with time.Its value is larger, and moisture is larger, otherwise moisture is less.Each time point water content (T behind water content difference (Δ T)=use product
hWater content (T before)-the use product
0).
The moisture of table 3 sample sets and the difference of initial value
Sample uses after 1 hour and compares with the blank group, and the moisture of sample sets obviously increases; Within 4 hours test periods, the moisture of sample sets 4 is higher than blank group always.
(2) hydration rate changes
Hydration rate=(sample sets moisture-initial moisture)/initial moisture
The moisture of table 4 sample sets over time with its initial value T assay
| Time (h) | 0 | 1 | 2 | 4 |
| T value | ?-- | 4.476 | 3.916 | 3.665 |
| The P value | -- | <0.05 | <0.05 | 0.001<0.05 |
The result: analysis result sees accompanying drawing 5 for details.
As seen from Figure 5, the hydration rate of blank group changes relatively stable, and sample sets is after sample uses 1 hour, and its hydration rate is organized apparently higher than blank, and reaches the highest; The hydration rate of sample sets is higher than blank group always in 4 hours.Compare with the blank group, sample has obvious moisturizing effect, and lasting enrich the water is good.
As seen from Table 4, within 4 hours test periods, sample was smeared after 1 hour in tested position, the moisture at tested position obviously raise (p＜0.05), namely from 1 hour significant difference that comes into existence, the moisture in its 4 hours is apparently higher than initial value (p＜0.05).
(3) relative moisture increment rate
The relative increment rate of moisture, namely moisturizing rate=(sample sets moisture-blank group moisture)/blank is organized moisture relatively; This value is larger, illustrates that the relative blank sample moistening effect of test specimen is more obvious.
The result: analysis result sees accompanying drawing 6 for details.
As seen from Figure 6, sample was smeared after 1 hour in tested position, and the relative moisture increment rate of sample sets obviously raises, and within 4 hours test periods, the relative increment rate of the moisture of sample sets be always on the occasion of, the moisturizing successful of sample sets is described with respect to the blank group.
(4) moisture loss situation
The moisture loss variation is reflected in test period Experimental Area moisture loss change with time.Its value is less, and moisture loss is fewer, and the lock outlet capacity is stronger; Otherwise the lock outlet capacity is more weak.Each time point fluid loss (T behind fluid loss difference (Δ T)=use product
hFluid loss (T before)-the use product
0).
The fluid loss of table 5 sample sets and the difference of initial value
Sample uses after 1 hour and compares with the blank group, and the water loss amount of sample sets obviously reduces; Within 4 hours test periods, the water loss amount of sample sets is lower than blank group always.
(5) moisture loss rate
Percentage of water loss=(sample sets water loss amount-initial water loss amount)/initial water loss amount
This value is less, illustrates that moisture loss is fewer, and test specimen lock water effect is more obvious.
The result: analysis result sees accompanying drawing 7 and table 6 for details.
As seen from Figure 7, within test period, sample was smeared after 1 hour in tested position, and the percentage of water loss of sample sets is starkly lower than blank group; Within 4 hours test periods, the percentage of water loss of sample sets is lower than blank group always, and is negative value always, and sample used after 4 hours, and the percentage of water loss at tested position reaches minimum; Sample is compared with the blank group, and instant lock water effect is arranged, and continues lock outlet capacity good (p＜0.05).
Table 6 sample water loss amount over time with its initial value T assay
| Time (h) | 0 | 1 | 2 | 4 |
| T value | -- | -3.129 | -4.948 | -5.388 |
| The P value | -- | 0.004<0.05 | <0.05 | 0<0.05 |
As seen from Table 6, within 4 hours test periods, sample was smeared after 1 hour in tested position, and the fluid loss at tested position obviously reduces (p＜0.05), namely from 1 hour significant difference that comes into existence, the water loss amount in its 4 hours is starkly lower than initial value (p＜0.05).
(6) the relative scatter ratio, scatter and disappear ratio of moisture
Relative percentage of water loss=(sample sets water loss amount-blank water loss amount)/blank water loss amount, this value is less, illustrates that moisture loss is fewer, and test specimen lock water effect is more obvious.
The result: analysis result sees accompanying drawing 8 for details
As seen from Figure 8, after using sample, the relative percentage of water loss of sample sets be negative value always, and the trend that tapers off, i.e. relative blank the group, and the fluid loss in the sample sets 4 hours obviously reduces.
The result
Show according to above result of the test:
1, within 4 hours test periods, after sample was used at tested position, sample used after 1 hour, and there is significant difference (p＜0.05) in the relative initial value of skin moisture content, and the ability of instant moisturizing is namely arranged, and successful; Continue enrich the water good (p＜0.05) in 4 hours.
2, within 4 hours test periods, after sample was used at tested position, sample used after 1 hour, and there is significant difference (p＜0.05) in the relative initial value of skin fluid loss, and the ability of instant lock water is namely arranged, and successful; Continue lock outlet capacity good (p＜0.05) in 4 hours.
Two, the zest test result of eye skin care item of the present invention
Experimental technique:
With reference to ECVAM DB-ALM:INVITTOX protocol; Red Blood Cell Test System INVITTOX n ° 37, hereinafter to be referred as RBC Test System.
Red Blood Cell(RBC) Test System is the irritating test method of authoritative test products that European alternative method authentication center authenticates, the Draize animal experiment before the method is intended to substitute.
The ultimate principle of RBC Test System is to measure the damage of chemicals cell membrane and the change of the cell membrane permeability that therefore causes, estimate the degree of injury of cell membrane by the amount of measuring the hemoglobin spill from erythrocyte, this degree of injury has directly related property with the zest of product.
The absorbance of the erythrocyte suspension after the effect of use spectrophotometry chemicals can calculate hemolysis rate and H
50Value (even concentration value of the tested material of 50% erythrocyte generation haemolysis).This H
50Preferably concordance of Correlation analysis showed is carried out in the result that value and two kinds of standards of grading of Draize test are maximum average value (MAS) and 24h threshold and the scoring of composition parameter (cornea, conjunctiva, iris) thereof.Under same concentrations, the hemolysis rate of sample is higher to show that the zest of sample is stronger.
The product zest detects: according to the stimulation of product to the erythrocyte cell membrane, can make the erythrocyte film rupture, the haemolysis that acquires a certain degree.Under same concentrations, the hemolysis rate of product is higher to show that the zest of product is stronger.
Product irritation detection: the PBS system of setting up raw material and erythrocyte; suppose that raw material has to a certain degree protective effect to erythrocyte; adding can reach the surfactant SDS of the particular volume accumulated amount of 50% haemolysis; erythrocyte in the stimulation system; under 530nm, measure absorbance; absorbance is larger, illustrates that the cell hemolysis rate is higher, and the irritation ability of raw material is more weak.Absorbance is less, illustrates that the cell hemolysis rate is lower, and the irritation ability of raw material is stronger.
Experiment content
1) preparation and the storage of erythrocyte (RBC): fresh animal blood splendid attire is in the vinyon container, with the anticoagulant mixing.Immediately the mixing blood sample is incubated in couveuse.Transport laboratory in stipulated time back, if blood sample is not polluted, the time can prolong.
Blood sample with the aseptic centrifuge tube packing of polyethylene gathers under the room temperature, washes blood sample repeatedly with buffer.This process can be removed a large amount of leukocyte, blood plasma and yellow fragment.The blood sample of handling well, sealing, Refrigerator store is for subsequent use.
Before the experiment, the RBC taking-up is stable at room temperature, stand-by take PBS accent RBC final concentration as normal concentration, whole process sterile working.
2) RBCH
50Measure: according to stipulating in the RBC Test System method, gathered erythrocytic integrity with mass ratio by 0.1% sodium lauryl sulphate (SDS) detection, examine under a microscope simultaneously erythrocytic form and cell membrane integrity.Determine to cause the H of erythrocyte hemolysis
50
3) sample autostimulation detection: according to stipulating in the RBC Test System method, sample (the eye moisturizing Skin whitening care cosmetics that the arbitrary embodiment of the present invention makes) is used the PBS(phosphate buffer) be diluted to respectively the liquid of final concentration 1.0%, with the zest of its test sample.
Experiment is divided into 2 groups: 1. positive controls; 2. specimen group
The comparison of positive controls and specimen group can characterize the zest of this sample.
Experimental result:
1, the assessment of RBC integrity
According to stipulating in the RBC Test System method, detect by 0.10% sodium lauryl sulphate (SDS) with mass ratio and to be gathered erythrocytic integrity, examine under a microscope simultaneously erythrocytic form and cell membrane integrity.
The results are shown in Figure 5 and Fig. 6, add SDS among Fig. 5 and be: SDS content is 0.10% PBS diluent.
Can get this time erythrocytic H by Fig. 9
50Be about 28.7 * 10
-6, meet regulation (29.4 ± 3.7) * 10 in the Test System standard
-6
Figure 10 is presented at microscopically, and red cell morphology and cell membrane are all complete.
2, sample stimulus property testing
When the concentration of sample in system is 4000 μ g/ml, the erythrocyte haemolysis does not occur, hemolysis rate is 0.92%, is lower than 20%, i.e. H
50The H of 4000 μ g/ml(SDS
50=26.1 μ g/ml) time, sample does not make erythrocyte that obvious haemolysis occurs.
When the concentration of sample in system is 8000 μ g/ml, the erythrocyte haemolysis does not occur, hemolysis rate is 0.37%, is lower than 20%, i.e. H
50The H of 8000 μ g/ml(SDS
50=26.1 μ g/ml) time, sample does not make erythrocyte that obvious haemolysis occurs.
Three, human body patch experimental result
Experimentation: the experimenter amounts to 30 people, and (meet " diagnostic criteria of cosmetics contact dermatitis and treatment principle " included in, exclusion standard) determined in concrete sex composition and age structure at random.
Select qualified patch material, sample (the eye moisturizing Skin whitening care cosmetics that the arbitrary embodiment of the present invention makes) is put into speckle examination device, and (amount of samples is that 0.020g～0.025g), control wells is blank (declining any material).Sample and blank all are affixed on experimenter's the bent side of forearm, make it to stick equably on skin with light pressure of palm, continue 24h.Interval 30min behind the removal patch device observes dermoreaction after impression disappears.24h and 48h observe once respectively again behind the patch test, observe dermoreaction.
Criterion:
Judge according to " diagnostic criteria of cosmetics contact dermatitis and treatment principle " classification: tested position is reactionless to be (0), skin light red speckle occurs and is (1), skin erythema, infiltration or pimple occur and is (2), edematous erythema appears in skin, pimple is (3), and it is (4) that remarkable redness, companion's pimple or bulla appear in skin.
Occur the number of 2 grades of skin adverse reactions in 30 examples more than 2 examples, or any 1 example occurs more than 3 grades or 3 grades during skin adverse reaction, judge that tested material has untoward reaction to human body.
Concrete outcome is estimated as shown in the table:
Table 7 is removed 0.5 hour observed result of speckle examination device
| Sample | Negative reaction (0) | Light red speckle, xerosis cutis, fold (1) | Erythema, edema, pimple, welt, desquamation crack (2) | Obvious erythema, edema, vesicle (3) | Severe erythema, edema, bulla, erosion, pigementation or hypopigmentation, acne sample change (4) |
| Blank | 29 | 1 | 0 | 0 | 0 |
| The | 30 | 0 | 0 | 0 | 0 |
Table 8 is removed 24 hours observed results of speckle examination device
| Sample | Negative reaction (0) | Light red speckle, xerosis cutis, fold (1) | Erythema, edema, pimple, welt, desquamation crack (2) | Obvious erythema, edema, vesicle (3) | Severe erythema, edema, bulla, erosion, pigementation or hypopigmentation, acne sample change (4) |
| | 30 | 0 | 0 | 0 | 0 |
| The | 30 | 0 | 0 | 0 | 0 |
Table 9 is removed 48 hours observed results of speckle examination device
| Sample | Negative reaction (0) | Light red speckle, xerosis cutis, fold (1) | Erythema, edema, pimple, welt, desquamation crack (2) | Obvious erythema, edema, vesicle (3) | Severe erythema, edema, bulla, erosion, pigementation or hypopigmentation, acne sample change (4) |
| | 30 | 0 | 0 | 0 | 0 |
| The | 30 | 0 | 0 | 0 | 0 |
Experimental result shows: eye Skin whitening care cosmetics of the present invention has no adverse reaction to human body.
The above embodiment is the preferred embodiment that proves absolutely that the present invention lifts, and protection scope of the present invention is not limited to this.Being equal to that those skilled in the art do on basis of the present invention substitutes or conversion, all within protection scope of the present invention.Protection scope of the present invention is as the criterion with claims.
Claims (10)
1. an eye moisturizing Skin whitening care cosmetics is characterized in that, contains the composition of following parts by weight:
α-mannan 30-60;
Poria cocos sclerotium extract 0.01-0.5;
Radix Angelicae Sinensis polysaccharide 0.1-5;
Radix Angelicae Sinensis total organic acids 0.1-5;
Rhizoma Atractylodis Macrocephalae root extract 0.1-6;
Eye encloses agent 40-75.
2. eye moisturizing Skin whitening care cosmetics according to claim 1 is characterized in that, described eye moisturizing Skin whitening care cosmetics is gel, also contains the composition of following parts by weight:
Deionized water 600-900;
Glycerol 20-45;
Butanediol 30-50;
Xanthan gum 0.1-5;
Carbomer U20 4-8;
Pantothenylol 2-8;
EDTA disodium 0.1-3;
Two-PEG-18 methyl ether dimethylsilane 3-7.
3. eye moisturizing Skin whitening care cosmetics according to claim 2 is characterized in that, also contains the composition of following parts by weight: stimulation inhibitory factor 10-40.
4. eye moisturizing Skin whitening care cosmetics according to claim 2 is characterized in that, also contains the composition of following parts by weight: antipruritic and antipruritic agent 10-50.
5. eye moisturizing Skin whitening care cosmetics according to claim 2 is characterized in that, also contains the composition of following parts by weight: the mixture 0.5-5 of Methylisothiazolinone and any ratio of iodine propilolic alcohol butyl mephenesin Carbamate.
6. the preparation method of eye moisturizing Skin whitening care cosmetics claimed in claim 2 is characterized in that, comprises the steps:
(1) with described amount glycerol, butanediol disperse yellow virgin rubber, add entry and stir, for subsequent use;
(2) water intaking adds in the emulsion tank, and carbomer U 20 evenly is sprinkled upon water surface, is dipped to water surface without white powder, and is stand-by;
(3) described amount α-mannan, EDTA disodium and water are added in step (2) the gained mixture through 200 eye mesh screens;
(4) after described amount pantothenylol is added to the water heating for dissolving, add in step (3) the gained mixture through 200 eye mesh screens;
(5) described poria cocos sclerotium extract, Radix Angelicae Sinensis polysaccharide, Radix Angelicae Sinensis total organic acids, Rhizoma Atractylodis Macrocephalae root extract and two-PEG-18 methyl ether dimethylsilane are added to the water heating for dissolving fully after, add in step (4) the gained mixture through 200 eye mesh screens;
(6) step (1) gained mixture is added in step (5) the gained mixture through 200 eye mesh screens, the vacuum mix and blend is heated to 80 ~ 85 ℃, insulation, and stirring is cooled to below 45 ℃;
(7) step (6) gained mixture is crossed 200 eye mesh screens, transfers pH to 3.5 ~ 8.5, and then vacuum stirring adds the pleasing to the eye agent that encloses, and vacuum stirring is cooled to 38 ℃, more than the ageing 24h.
7. the preparation method of eye moisturizing Skin whitening care cosmetics according to claim 6 is characterized in that, in the step (3) also with stimulation inhibitory factor behind 200 mesh sieves, add in step (2) the gained mixture.
8. the preparation method of eye moisturizing Skin whitening care cosmetics according to claim 6 is characterized in that, in the step (3) also with antipruritic and antipruritic agent behind 200 mesh sieves, add in step (2) the gained mixture.
9. the preparation method of eye moisturizing Skin whitening care cosmetics according to claim 6, it is characterized in that, step (6) gained mixture in the step (7), cross 200 eye mesh screens, transfer pH to 3.5 ~ 8.5, vacuum stirring, then add the pleasing to the eye mixture that encloses agent and Methylisothiazolinone and any ratio of iodine propilolic alcohol butyl mephenesin Carbamate, vacuum stirring is cooled to 38 ℃, more than the ageing 24h.
10. the preparation method of eye moisturizing Skin whitening care cosmetics according to claim 6 is characterized in that, step (6) gained mixture in the step (7) is crossed 200 eye mesh screens, transfers pH to 3.5 ~ 8.5 with the NaOH aqueous solution.
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| CN2012103175441A CN102860975A (en) | 2012-08-31 | 2012-08-31 | Skin-whitening and moisture-preserving skin-care product for eyes and preparation method for skin-whitening and moisture-preserving skin-care product |
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| CN2012103175441A CN102860975A (en) | 2012-08-31 | 2012-08-31 | Skin-whitening and moisture-preserving skin-care product for eyes and preparation method for skin-whitening and moisture-preserving skin-care product |
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| CN102860975A true CN102860975A (en) | 2013-01-09 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104138347A (en) * | 2014-08-15 | 2014-11-12 | 马应龙药业集团股份有限公司 | Chinese herbal whitening composition, facial mask and preparation method of facial mask |
| CN105326779A (en) * | 2015-11-24 | 2016-02-17 | 广州市花安堂生物科技有限公司 | Moisturizing, brightening and whitening hair gel and preparation method thereof |
| JPWO2015152384A1 (en) * | 2014-04-03 | 2017-04-13 | ポーラ化成工業株式会社 | Melanin production inhibitor comprising D-pantothenyl alcohol, and whitening cosmetic comprising the melanin production inhibitor |
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| CN102319198A (en) * | 2011-08-12 | 2012-01-18 | 马应龙药业集团股份有限公司 | Whitening eye plaster and preparation method thereof |
| CN102319197A (en) * | 2011-08-12 | 2012-01-18 | 马应龙药业集团股份有限公司 | Wrinkle-removal eye paste and preparation method thereof |
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| CN102319198A (en) * | 2011-08-12 | 2012-01-18 | 马应龙药业集团股份有限公司 | Whitening eye plaster and preparation method thereof |
| CN102319197A (en) * | 2011-08-12 | 2012-01-18 | 马应龙药业集团股份有限公司 | Wrinkle-removal eye paste and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPWO2015152384A1 (en) * | 2014-04-03 | 2017-04-13 | ポーラ化成工業株式会社 | Melanin production inhibitor comprising D-pantothenyl alcohol, and whitening cosmetic comprising the melanin production inhibitor |
| CN112402285A (en) * | 2014-04-03 | 2021-02-26 | 宝丽化成工业有限公司 | Whitening cosmetic and use of composition containing D-panthenol in preparation of whitening cosmetic |
| CN104138347A (en) * | 2014-08-15 | 2014-11-12 | 马应龙药业集团股份有限公司 | Chinese herbal whitening composition, facial mask and preparation method of facial mask |
| CN105326779A (en) * | 2015-11-24 | 2016-02-17 | 广州市花安堂生物科技有限公司 | Moisturizing, brightening and whitening hair gel and preparation method thereof |
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Application publication date: 20130109 |