CN115154403A

CN115154403A - Skin repair composition and preparation method and application thereof

Info

Publication number: CN115154403A
Application number: CN202211028535.0A
Authority: CN
Inventors: 李雄山
Original assignee: Guangzhou Archimedes Biotechnology Co ltd
Current assignee: Archimedes Guangzhou Cosmetics Research Co ltd
Priority date: 2022-08-25
Filing date: 2022-08-25
Publication date: 2022-10-11
Anticipated expiration: 2042-08-25
Also published as: CN115154403B

Abstract

The invention provides a skin repair composition and a preparation method and application thereof, wherein the skin repair composition comprises a deformed vesicle, water and active substances loaded in the deformed vesicle, and the active substances comprise DNA sodium, nicotinamide adenine dinucleotide, ceramide and natural product extracts. The skin repair composition provided by the invention can increase the transdermal absorption of the skin by loading the active substance in the deformed vesicle; the barrier repair effect of the skin is enhanced by four factors of relieving, diminishing inflammation, supplementing and rebuilding; the active substances are matched with each other, have a synergistic effect, have a strong moisturizing effect, enhance the skin elasticity, enhance the skin barrier function, improve the self-protection capability of the skin, reduce the skin sensitivity, have fresh and cool skin feeling without greasiness, and can effectively improve the skin problems of patients with hormone dependent dermatitis.

Description

Skin repair composition and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a skin repair composition as well as a preparation method and application thereof.

Background

The world health organization (WTO) has reported that: "in modern people, skin is less than 20% healthy, more than 20% sick, and in between 70% of sub-healthy skin population".

Sensitive skin is a sub-healthy state of the skin, and is mainly characterized in that the tolerance threshold of the skin is lowered, and the skin is easily stimulated by various internal and external factors to have uncomfortable feelings such as pruritus, burning, irritation, dryness or tightness, and occasionally accompanied by erythema, desquamation and the like. Factors causing skin sensitivity mainly include:

(1) Drying and water shortage: after the moisture on the surface layer of the skin is lost, the skin can have the problems of roughness, pruritus, chapping, desquamation and the like; if the skin is lacked for a long time, the skin is cracked, so that the barrier is damaged, and the health of the skin is influenced;

(2) External pollution: the stimulation of external adverse environments such as ultraviolet rays, automobile exhaust, dust in air, microorganisms and the like easily causes pigment precipitation, water-oil imbalance and sensitive stimulation reaction in a short time; the collagen in the skin is easily excessively lost, the cells are damaged, the immunity of the skin is weakened, and the aging process of the skin is accelerated;

(3) Poor skin care: the skin is excessively cleaned and the disinfection product is used, the balance of flora on the surface of the skin is easily influenced, the skin barrier is easily damaged due to excessive cutin removal, and hormone medicines are irregularly used for a long time; the long-term use of the hormone-containing cosmetics by consumers can generate dependence on the hormone, and can induce non-suppurative inflammatory reaction of skin to cause contact dermatitis once the cosmetics are stopped, and phenomena of facial telangiectasia erythema, pimple, facial skin atrophy, rosacea-like skin rash, pigmentation and the like, namely hormone-dependent dermatitis, appear.

(4) Physiological reasons are as follows: the self-metabolism is reduced, the body immunity is reduced, the skin tolerance is reduced, skin cells are easy to be stimulated by the outside, and the phenomena of redness, fever, pruritus, stabbing pain, erythra and the like are generated.

Therefore, how to provide a composition with soothing effect, so that the composition can enhance the skin barrier function and repair damaged skin, is a problem to be solved at present.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a skin repair composition and a preparation method and application thereof. The skin repair composition provided by the invention increases the repair effect of skin from four factors of relieving, diminishing inflammation, supplementing and rebuilding, has the characteristics of naturalness, mildness and high safety, has a strong barrier repair effect, is easy to be absorbed by skin, has a wide application range, can effectively improve the skin problem of a patient suffering from hormone dependent dermatitis, and can repair the skin damage problem after skin metabolism.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a skin repair composition comprising an amoebosome, water, and an active agent loaded in the amoebosome, the active agent comprising sodium DNA, nicotinamide adenine dinucleotide, ceramide, and a natural product extract.

Cholesterol, ceramide and free fatty acid are the most main components of the synthetic sebum membrane, and the skin repair composition is prepared from four factors of relieving, diminishing inflammation, supplementing and reconstructing according to the characteristics of the sebum membrane. The skin modification composition provided by the invention has the characteristics of being natural and mild, high in safety, strong in barrier repair effect, easy to absorb by skin, wide in application range and capable of effectively improving the skin problems of patients suffering hormone-dependent dermatitis.

In the present invention, the skin repair composition comprises, in parts by weight, 11 to 27 parts (for example, 11 parts, 13 parts, 15 parts, 17 parts, 19 parts, 21 parts, 23 parts, 25 parts, 27 parts, etc.) of the deformed vesicle, 2 to 54 parts (for example, 2 parts, 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, 54 parts, etc.) of water, and 35 to 88 parts (for example, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, 88 parts, etc.) of an active substance loaded in the deformed vesicle.

Preferably, the active substance comprises 0.5-1 part (for example, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like) of DNA sodium, 0.5-1 part (for example, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like) of nicotinamide adenine dinucleotide, 2.5-5 parts (for example, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like) of ceramide and 17-40 parts (for example, 17 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts and the like) of natural product extract by weight.

In the present invention, the natural product extract includes any one or a combination of at least two of madecassoside, leontopodium alpinum callus extract, hypsizygus marmoreus extract, thermus thermophilus fermentation product, gentian root extract, or chamomile flower extract, preferably a combination of madecassoside, leontopodium alpinum callus extract, hypsizygus marmoreus extract, thermus thermophilus fermentation product, gentian root extract, and chamomile flower extract.

Preferably, the first and second liquid crystal display panels are, the natural product extract includes 5 to 15 parts (e.g., 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc.) of madder callus extract (e.g., 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc.), 0.5 to 1 part (e.g., 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, etc.) of hypecoum extract, 1 to 3 parts (e.g., 1 part, 1.5 part, 2 parts, 2.5 parts, 3 parts, etc.) of thermophilic bacteria fermentation product, 0.5 to 1 part (e.g., 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, 0.5 part, 2.5 part, 3 parts, etc.) of gentian extract, 0.5 to 1 part (e.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, etc.) of mother extract, 0.5 parts, 0.6 parts, 0.9 parts, etc.) and 0.5 parts of chrysanthemum extract, 0.9 parts, etc.) in parts by weight, etc. parts by weight.

In the present invention, the ceramide includes any one of or a combination of at least two of ceramide NS/ceramide NG, ceramide NP, ceramide EOP, ceramide AS, or ceramide AP, preferably a combination of ceramide NS/ceramide NG, ceramide NP, ceramide EOP, ceramide AS, and ceramide AP.

Preferably, the ceramide includes, in parts by weight, 0.5 to 1 part (e.g., may be 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, etc.) of ceramide AS, and 0.5 to 1 part (e.g., may be 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, etc.) of ceramide AP.

In the present invention, the active substance further includes a fat.

Preferably, the oil comprises any one or a combination of at least two of olive fruit oil, argan nut oil, squalane, shea butter or macadamia nut seed oil, preferably a combination of olive fruit oil, argan nut oil, squalane, shea butter and macadamia nut seed oil.

The skin repair composition provided by the invention is natural and mild, takes olive oil, argania spinosa kernel oil, squalane, shea butter and macadamia nut seed oil as basic grease, is used as an integral framework of the composition, enhances the effect of repairing damaged skin of the composition, has high safety, has a strong moisturizing effect, is easy to absorb by skin, has fresh and non-greasy skin feeling when used, and can effectively improve the skin problem of a patient suffering from hormone-dependent dermatitis.

Preferably, the first and second electrodes are formed of a metal, the oil comprises, by weight, 5 to 10 parts (for example, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc.), 5 to 10 parts (for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.) of shea butter, 1 to 5 parts (for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.) and 1 to 5 parts (for example, 1 to 5 parts, 2 parts, 3 parts, 4 parts, 5 parts, etc.) of macadamia seed oil.

Preferably, the active substance further comprises 1, 2-hexanediol.

Preferably, the active substance also comprises 2-5 parts (for example, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like) of 1, 2-hexanediol.

In the invention, the raw materials for preparing the proteosome comprise PEG/PPG-14/7 dimethyl ether, polyglycerol ester surfactant, sorbitan ester surfactant, cetyl alcohol phosphate, honokiol and magnolol.

Preferably, the raw materials for preparing the proteosome comprise 2-5 parts (such as 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like) of PEG/PPG-14/7 dimethyl ether, 4-10 parts (such as 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like) of polyglycerol ester surfactants, 2-5 parts (such as 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like) of sorbitan ester surfactants, 1-3 parts (such as 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts and the like) of cetyl alcohol phosphate ester, 1-2 parts (such as 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts and the like) of magnolol and 1-2 parts (such as 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts and the like) of magnolol.

Preferably, the polyglycerol ester surfactant comprises a polyglycerol-6 ester and/or polyglycerol-10 oleate of wild almond oil, preferably a combination of polyglycerol-6 ester and polyglycerol-10 oleate of wild almond oil.

Preferably, the sorbitan ester surfactant comprises sorbitan palmitate.

Preferably, the mass ratio of the magnolol to the polyglycerol ester-based surfactant is 1 (2-10) (wherein "2-10" may be 2, 3, 4, 5, 6, 7, 8, 9, 10, etc.).

In the invention, glycerin and natural oil are utilized to form a bilayer through a chemical synthesis method, so as to obtain polyglycerin (wild almond oil polyglycerin-6 esters and polyglycerin-10 oleate); the sorbitan ester surfactant is added to form a bilayer of the deformed vesicle, so that membrane permeability, encapsulation efficiency and bilayer rigidity are influenced; adding cetyl phosphate to ensure stability; honokiol and magnolol are added to adjust the stability, shape, size and other properties of the bilayer, which are determined by the mass ratio of magnolol and polyglycerol-based surfactant.

In the deformed vesicle, the bilayer is completely free of phospholipids and contains only single-chain surfactants, with maximum elasticity and deformability, and thus maximum transdermal capacity.

Because the deformed vesicles are highly hydrophilic vesicles, attempts are always made to avoid dehydration; when applied to the skin, vesicles tend to penetrate the skin barrier, moving to a deep layer rich in moisture, ensuring adequate hydration of the vesicle itself. The mechanism of penetration is that evaporation of water produces an "osmotic gradient" when the deformed vesicles are applied to the skin surface, and thus the transport of these elastic vesicles is not concentration-dependent.

In a second aspect, the present invention provides a method of preparing a skin rejuvenating composition according to the first aspect, the method comprising the steps of: mixing the deformed vesicle, water and the active substance, stirring and homogenizing to obtain the skin repair composition.

In the present invention, the mixing temperature is 35-45 ℃ (for example, 35 ℃, 37 ℃, 39 ℃, 41 ℃, 43 ℃, 45 ℃ and the like can be used).

Preferably, the stirring speed is 50-70rpm (for example, 50rpm, 53rpm, 56rpm, 59rpm, 61rpm, 64rpm, 67rpm, 70rpm, etc.) and the time is 0.8-1.2h (for example, 0.8h, 0.9h, 1h, 1.1h, 1.2h, etc.).

Preferably, the homogenizing speed is 4500-5500rpm (e.g. 4500rpm, 4700rpm, 4900rpm, 5000rpm, 5200rpm, 5400rpm, 5500rpm, etc.) and the time is 4-6min (e.g. 4min, 4.3min, 4.6min, 4.9min, 5.1min, 5.4min, 5.7min, 6min, etc.).

In the present invention, the method for preparing the deformed vesicle comprises the following steps:

(1) Mixing polyglycerol ester surfactant, sorbitan ester surfactant and cetyl phosphate, and stirring to obtain bilayer;

(2) And (2) mixing the bilayer obtained in the step (1), PEG/PPG-14/7 dimethyl ether, honokiol and magnolol, and stirring to obtain the deformed vesicle.

Preferably, in step (1), the temperature of the mixing is 55-65 ℃ (for example, 55 ℃, 57 ℃, 59 ℃, 61 ℃, 63 ℃, 65 ℃ and the like can be used).

Preferably, in step (1), the rotation speed of the stirring is 45-55rpm (for example, 45rpm, 47rpm, 49rpm, 51rpm, 53rpm, 55rpm, etc.), and the time is 20-40min (for example, 20min, 23min, 26min, 29min, 31min, 34min, 37min, 40min, etc.).

Preferably, in step (2), the temperature of the mixing is 40-50 ℃ (for example, 40 ℃, 42 ℃, 46 ℃, 48 ℃, 50 ℃ and the like can be realized).

Preferably, in step (2), the rotation speed of the stirring is 35-45rpm (for example, 35rpm, 37rpm, 39rpm, 41rpm, 43rpm, 45rpm, etc.), and the time is 20-40min (for example, 20min, 23min, 26min, 29min, 31min, 34min, 37min, 40min, etc.).

In a third aspect, the present invention provides a use of the skin repair composition according to the first aspect in the preparation of a skin care product.

Preferably, the skin care product comprises a cream and/or a serum.

Compared with the prior art, the invention has the following beneficial effects:

(1) The skin repair composition provided by the invention can increase the transdermal absorption of the skin by loading the active substance into the deformed vesicle; the four factors of relieving, diminishing inflammation, supplementing and reconstructing are adopted, and the barrier repair effect of the skin is enhanced;

(2) The active substances are matched with each other, have a synergistic effect, have a strong moisturizing effect, enhance the skin elasticity, enhance the skin barrier function, improve the self-protection capability of the skin, reduce the skin sensitivity, have fresh and cool skin feeling without greasiness, and can effectively improve the skin problems of patients suffering from hormone dependent dermatitis;

(3) All components in the active substance are mutually matched and have synergistic effect, so that nutrients are provided for basal cells of the skin, the division of the basal cells of the skin is promoted, the formation of new cutin is accelerated, and the effect of repairing the damaged skin can be finally achieved;

(4) The skin repair composition provided by the invention is high in safety, natural and mild;

(5) The preparation method of the skin repair composition provided by the invention has the characteristics of simple operation, convenient control, high production efficiency and low production cost, and can be used for large-scale production.

(6) The skin repair composition provided by the invention can improve the hydration of the skin, and has the advantages of exogenous hydration of stratum corneum (large amount of water), dehydration of stratum corneum (water evaporation), compression rearrangement of stratum corneum structure (compact and ordered), enhancement of stratum corneum barrier function (better resistance to external stimuli)/higher light transmittance (smooth and transparent appearance).

Drawings

FIG. 1 is a diagram of an in vitro transdermal absorption experiment of free fluorescein.

FIG. 2 is a diagram of an in vitro transdermal absorption experiment of fluorescein encapsulated in a deformed vesicle.

FIG. 3 is a diagram showing the stratum corneum alignment process.

Detailed Description

The technical solution of the present invention is further described below by way of specific embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Example 1

The embodiment provides a skin repair composition, which comprises the following components in parts by weight:

the preparation method of the skin repair composition comprises the following steps:

(1) Mixing oleum Armeniacae amarum polyglycerol-6 esters, polyglycerol-10 oleate, sorbitan palmitate and cetyl phosphate at 60 deg.C, stirring at 50rpm for 30min to obtain bilayer;

(2) Mixing the bilayer obtained in the step (1), PEG/PPG-14/7 dimethyl ether, honokiol and magnolol at 45 ℃, and stirring at the rotating speed of 40rpm for 30min; obtaining the deformed vesicle;

(3) Mixing the deformed vesicle obtained in the step (2), water and an active substance at 40 ℃, and stirring for 1h at the rotating speed of 60 rpm; and homogenizing at 5000rpm for 5min to obtain the skin repairing composition.

Example 2

(1) Mixing oleum Armeniacae amarum polyglycerol-6 esters, polyglycerol-10 oleate, sorbitan palmitate and cetyl phosphate at 55 deg.C, stirring at 55rpm for 20min to obtain bilayer;

(2) Mixing the bilayer obtained in the step (1), PEG/PPG-14/7 dimethyl ether, honokiol and magnolol at 40 ℃, and stirring at the rotation speed of 45rpm for 20min; obtaining the deformed vesicle;

(3) Mixing the deformed vesicle obtained in the step (2), water and active substances at 35 ℃, and stirring for 1.2h at the rotating speed of 50 rpm; homogenizing at 4500rpm for 4min to obtain the skin repairing composition.

Example 3

(1) Mixing oleum Armeniacae amarum polyglycerol-6 esters, polyglycerol-10 oleate, sorbitan palmitate and cetyl phosphate at 65 deg.C, stirring at 45rpm for 40min to obtain bilayer;

(2) Mixing the bilayer obtained in the step (1), PEG/PPG-14/7 dimethyl ether, honokiol and magnolol at 50 ℃, and stirring at the rotation speed of 35rpm for 40min; obtaining the deformed vesicle;

(3) Mixing the deformed vesicle obtained in the step (2), water and active substances at 45 ℃, and stirring for 0.8h at the rotating speed of 70 rpm; and homogenizing at 5500rpm for 6min to obtain the skin repairing composition.

Example 4

This example provides a skin-repairing composition, which is different from example 1 only in that it does not contain madecassoside, the weight part of the edelweiss callus extract is increased to 20 parts, and the other components and preparation method are the same as example 1.

Example 5

This example provides a skin rejuvenating composition, which is different from example 1 only in that it does not contain alpine edelweiss callus extract, the weight part of madecassoside is increased to 20 parts, and other components and preparation method are the same as example 1.

Example 6

This example provides a skin repair composition, which is different from example 1 only in that it does not contain hypsizygus marmoreus extract, the weight part of thermus thermophilus fermentation product is increased to 2.4 parts, the weight part of gentian root extract is increased to 1.2 parts, the weight part of chamomile extract is increased to 1.2 parts, and other components and preparation methods are the same as example 1.

Example 7

This example provides a skin repair composition, which is different from example 1 only in that, without the fermentation product of thermus thermophilus, the parts by weight of the hypecoum extract are increased to 1.6 parts, the parts by weight of the gentian root extract are increased to 1.6 parts, the parts by weight of the chamomile flower extract are increased to 1.6 parts, and other components and preparation methods are the same as example 1.

Example 8

This example provides a skin-repairing composition, which is different from example 1 only in that, in the absence of gentian root extract, the parts by weight of hypecoum vulgare extract were increased to 1.2 parts, the parts by weight of thermus thermophilus fermentation product was increased to 2.4 parts, and the parts by weight of chamomile flower extract was increased to 1.2 parts, and the other components and preparation method were the same as example 1.

Example 9

This example provides a skin-repairing composition, which is different from example 1 only in that, in the absence of the chamomile flower extract, the parts by weight of the hypsizygus marmoreus extract were increased to 1.2 parts, the parts by weight of the thermus thermophilus fermentation product were increased to 2.4 parts, and the parts by weight of the gentian root extract were increased to 1.2 parts, and the other components and preparation method were the same as example 1.

Example 10

This example provides a skin rejuvenation composition that differs from example 1 only in that, in the absence of ceramide NS/ceramide NG, the weight fraction of ceramide NP is increased to 1 part, the weight fraction of ceramide EOP is increased to 1 part, the weight fraction of ceramide AS is increased to 1 part, the weight fraction of ceramide AP is increased to 1 part, and the other components and preparation method are the same AS example 1.

Example 11

This example provides a skin rejuvenation composition which differs from example 1 only in that, without ceramide NP, the weight fraction of ceramide NS/ceramide NG was increased to 1 part, the weight fraction of ceramide EOP was increased to 1 part, the weight fraction of ceramide AS was increased to 1 part, the weight fraction of ceramide AP was increased to 1 part, and the other components and preparation method were the same AS example 1.

Example 12

This example provides a skin rejuvenation composition that differs from example 1 only in that, without ceramide EOP, the parts by weight of ceramide NS/ceramide NG was increased to 1 part, the parts by weight of ceramide NP was increased to 1 part, the parts by weight of ceramide AS was increased to 1 part, the parts by weight of ceramide AP was increased to 1 part, and the other components and preparation method were the same AS example 1.

Example 13

This example provides a skin rejuvenating composition, which differs from example 1 only in that, without ceramide AS, the weight fraction of ceramide NS/ceramide NG was increased to 1 part, the weight fraction of ceramide NP was increased to 1 part, the weight fraction of ceramide EOP was increased to 1 part, the weight fraction of ceramide AP was increased to 1 part, and the other components and preparation method were the same AS example 1.

Example 14

This example provides a skin rejuvenation composition that differs from example 1 only in that, without ceramide AP, the weight fraction of ceramide NS/ceramide NG was increased to 1 part, the weight fraction of ceramide NP was increased to 1 part, the weight fraction of ceramide EOP was increased to 1 part, the weight fraction of ceramide AS was increased to 1 part, and the other components and preparation methods were the same AS example 1.

Example 15

This example provides a skin rejuvenating composition which differs from example 1 only in that olive fruit oil is not included, the parts by weight of argan nut oil is increased to 12 parts, the parts by weight of squalane is increased to 12 parts, and the other components and preparation method are the same as example 1.

Example 16

This example provides a skin repair composition that differs from example 1 only in that argania spinosa kernel oil is not included, the number of parts by weight of olive fruit oil is increased to 12 parts, the number of parts by weight of squalane is increased to 12 parts, and the other components and preparation method are the same as example 1.

Example 17

This example provides a skin rejuvenating composition which differs from example 1 only in that squalane is not present, the parts by weight of olive fruit oil is increased to 12 parts, the parts by weight of argan nut oil is increased to 12 parts, and the other components and preparation method are the same as example 1.

Example 18

This example provides a skin rejuvenating composition that differs from example 1 only in that it does not contain shea butter, increasing the parts by weight of macadamia nut seed oil to 8 parts, and the other components and preparation method are the same as example 1.

Example 19

This example provides a skin rejuvenating composition that differs from example 1 only in that no macadamia nut seed oil is included, the parts by weight of shea butter is increased to 8 parts, and the other components and preparation method are the same as example 1.

Comparative example 1

This comparative example provides a skin-repairing composition, which is different from example 1 only in that it does not contain sodium DNA, the weight part of nicotinamide adenine dinucleotide is increased to 1.6 parts, and the other components and the preparation method are the same as example 1.

Comparative example 2

This comparative example provides a skin repair composition, which is different from example 1 only in that nicotinamide adenine dinucleotide is not contained, the weight part of the DNA sodium is increased to 1.6 parts, and the other components and the preparation method are the same as example 1.

Comparative example 3

This comparative example provides a skin repair composition, which differs from example 1 only in that it does not contain deforming vesicles, and a method for preparing the skin repair composition comprising the steps of: mixing water and active substances at 40 deg.C, and stirring at 60rpm for 1h; and homogenizing at 5000rpm for 5min to obtain the skin repairing composition.

Test example

Test example 1

Deformed vesicle transdermal absorption test

Testing a sample: example 1 denatured vesicle obtained in step (2).

The test method comprises the following steps: in vitro transdermal test materials using isolated bama pig skin as an in vitro transdermal test material, saline-ethanol (1), pH 5.8 phosphate buffer solution-ethanol (1), pH 7.4 phosphate buffer solution-ethanol (1) ² Glass window composition) to determine the permeation and absorption rule of the deformed vesicle, and the LCMS/MS method is used to respectively determine the total absorption amount of the fluorescein in the receiving solution and the change behavior of the residual amount of the fluorescein in the pigskin along with the time, and evaluate the transdermal consistency of the test and the reference fluorescein.

FIG. 1 is a diagram of an in vitro transdermal absorption experiment of free fluorescein, and FIG. 2 is a diagram of an in vitro transdermal absorption experiment of fluorescein encapsulated in a deformed vesicle. From fig. 1 and 2, it can be seen that fluorescein encapsulated in deformed vesicles is much more permeable in the stratum corneum than free fluorescein.

Test example 2

Testing a sample: examples 1-19 and comparative examples 1-3 provide skin repair compositions.

The test method comprises the following steps:

the hair on the right hind foot was removed before test 1d by using 138 guinea pigs, male and female half, and the area of hair removed was about 2cm ² The guinea pigs were randomly divided into 23 groups each containing 6 guinea pigs and recorded as examples 1-19 groups, comparative examples 1-3 groups and blank groups, respectively.

In the test, 0.05mL of the skin-repairing composition prepared in examples 1 to 19 and comparative examples 1 to 3 was applied to the dehaired parts of guinea pigs in examples 1 to 19 and comparative examples 1 to 3 continuously for 2 days, and an equal amount of deionized water was applied to the blank group 1 time a day.

On day 3, histamine phosphate solutions of 0.01% (w/v), 0.02% (w/v), 0.03% (w/v), 0.04% (w/v), 0.05% (w/v), 0.06% (w/v), 0.07% (w/v), 0.08% (w/v), 0.09% (w/v), and 0.1% (w/v) were prepared with physiological saline, respectively.

Coarse sand paper is used for scratching the hair-removed part of the dorsum of the right back foot of the guinea pig, and the scratching area is about 1cm ² And locally applying the corresponding skin repair composition for 1 time, wherein the application volume is 0.05mL, applying an equal amount of deionized water to a blank group, after 10min, dropwise adding 0.05mL of 0.01% (w/v) histamine phosphate solution to the site of the scratch, observing whether the guinea pig returns to the head within 3min to lick the right hind paw, if the histamine phosphate solution does not exist, continuously dropwise adding 0.05mL of 0.02% (w/v) histamine phosphate solution to the site of the scratch, and observing whether the guinea pig returns to the head within 3min to lick the right hind paw. By analogy, the concentration is increased according to the gradient every 3min, 0.05mL of histamine phosphate solution is dripped every time until the guinea pig licks the right hind paw within 3 min. The cumulative total amount of histamine phosphate added dropwise at this time was taken as the itch threshold (μ g), the average value of the itch threshold of the same group of guinea pigs was calculated, and the data was collated, and the test results are shown in table 1:

TABLE 1

Group of	Itch threshold (mug)	Group of	Itch threshold (mug)
				Example 1	212.5	Example 13	181.6
Example 2	209.8	Example 14	182.0
				Example 3	210.4	Example 15	192.5
Example 4	163.2	Example 16	189.8
				Example 5	161.7	Example 17	173.5
Example 6	154.8	Example 18	179.9
				Example 7	163.4	Example 19	187.4
Example 8	156.2	Comparative example 1	182.1
				Example 9	153.8	Comparative example 2	145.6
Example 10	184.5	Comparative example 3	132.1
				Example 11	180.4	Blank group	45.2
Example 12	182.7

As is clear from the data in Table 1, the skin repair composition provided by the invention has a certain inhibiting effect on skin itch of guinea pigs caused by histamine phosphate.

As is clear from comparison between example 1 and examples 4 to 9, madecassoside, an extract from calluses of Leontopodium alpinum, an extract from Criste maritime, a fermentation product of Thermus thermophilus, an extract from Gentiana scabra Bunge root and an extract from Matricaria chamomilla flower were found to cooperate with each other to synergistically inhibit itch of skin of guinea pigs caused by histamine phosphate.

As can be seen from a comparison of example 1 with examples 10 to 14, ceramide NS/ceramide NG, ceramide NP, ceramide EOP, ceramide AS and ceramide AP cooperate with each other to synergistically enhance adhesion between keratinocytes, improve skin dryness and maintain skin barrier.

As can be seen by comparing example 1 with examples 15-19, the olive fruit oil, the argan nut oil, squalane, shea butter and macadamia nut oil cooperate with each other to synergistically enhance the efficacy of the composition in repairing damaged skin, are easily absorbed by the skin, and have a refreshing and non-greasy feel in use.

As is clear from the comparison between example 1 and comparative examples 1-2, DNA sodium and nicotinamide adenine dinucleotide promote the metabolic turnover of cells, and thus both have a certain inhibitory effect on skin itch of guinea pigs caused by histamine phosphate.

As can be seen from a comparison between example 1 and comparative example 3, loading an active substance in a deformed vesicle makes the active substance more easily absorbed by the skin, and has a stronger barrier repair effect.

Test example 3

Test of fading effect

Reference is made to the literature (Wang-neck, anti-allergic and antipruritic plant combined extract preparation process, efficacy and action pathway research [ D ].2015 ]) method recorded in the 2.2.6 skin irritation repair experiment.

The specific method comprises the following steps: the subjects were divided into a total of 115 persons, 23 groups of 5 persons each, of which 1 group was blank, and the remaining 22 groups were tested for the skin-repairing compositions provided in examples 1 to 19 and comparative examples 1 to 3, respectively, and the specific gender configurations were randomly determined. The exclusion conditions of the volunteers meet the inclusion and exclusion standards of the diagnosis standard and the treatment principle of the cosmetic contact dermatitis.

Selecting the inner sides of the left and right arms of a subject as tested parts, and circularly marking a square area with the specification of 2cm multiplied by 2 cm; washing the test area (same area) with clear water, sitting the subject still for 10min, measuring the blank value of skin red value with Mexamter MX18 (pigment test probe) of CK company in Germany, and entering the smearing experiment stage; stimulating the tested area with 50% capsaicin water solution (about 0.01 mL); continuously stimulating for 15min and 30min, and collecting numerical value; smearing a test sample (0.05 mL, diluting the concentration to 10 mg/mL) on a tested area, and performing data acquisition after 15min, 30min and 45 min; smearing 0.05mL of pure water on the blank control; and (4) counting the average value of the values measured at each time of the experimental part of each group of subjects, and analyzing the change of the red value.

The change of the skin red value is reflected in the change rule of the skin redness of the experimental area along with the time in the testing period. The greater the value, the greater the degree of redness of the skin.

Skin redness change rate (%) = [ (T-T) _{Blank value} )/T _{Blank value} ]×100％。

Difference = skin redness change rate after 30min of stimulation-skin redness change rate after 45min of sample use;

the test results are shown in table 2 below:

TABLE 2

As can be seen from the data in table 2, the skin-repairing composition provided by the present invention has a repairing effect on skin irritation. By loading the active substance in the deformed vesicle, the transdermal absorption of the skin can be increased. The DNA sodium, the nicotinamide adenine dinucleotide, the ceramide, the natural product extract and the grease in the active substances are matched with each other, so that the synergistic effect is achieved, and the repair effect on the skin is improved.

Test example 4

Test of moisturizing Effect

110 skin-healthy 18-40-year-old volunteers were selected, male and female in half ratio, and randomly divided into 22 groups, and the skin-repairing compositions provided in examples 1 to 19 and comparative examples 1 to 3 were used, respectively. After cleaning face of volunteer, the ratio of 2mg/cm ² The skin repair composition is weighed and then evenly smeared in a test area, the skin repair composition is used once after being washed on the face in the morning and continuously used for 30 days, and the water content of the skin of a volunteer before and after use is measured by a skin tester (MC 750, CK in Germany);

the moisture content of the facial skin of each subject was tested before use and after 30 days of continuous use, each data was tested three times, after taking the average value, the average value of each group was calculated, one digit after the decimal point was retained, and the rate of change of the moisture content of the skin was calculated according to the following formula:

change rate of moisture content of facial skin (%) = [ moisture content of facial skin 30 days after use-moisture content of facial skin before use)/moisture content of facial skin before use ] × 100%;

the test results are shown in table 3:

TABLE 3

As can be seen from the test results in table 3, the skin repair composition provided by the present invention can increase the transdermal absorption of the skin by loading the active substance into the deformed vesicles. The active substances are the mutual cooperation of DNA sodium, nicotinamide adenine dinucleotide, ceramide, natural product extract and grease, so that the synergistic effect is achieved, the facial water content is improved, and the skin moisturizing state is strengthened and restored.

As shown in fig. 3, the skin repair composition provided by the present invention can improve skin hydration, and exogenous hydration of stratum corneum (large amount of water) (moisture supplement) → stratum corneum dehydration (water evaporation) → stratum corneum structural compression rearrangement (compact order) → stratum corneum barrier function enhancement (better resistance to external stimuli)/transmittance is higher (smooth and transparent in appearance).

Test example 5 safety test

Cosmetic eye irritation test chick embryo chorioallantoic membrane test

Test samples: examples 1-19 and comparative examples 1-3 provide skin rejuvenation compositions.

The method comprises the following steps: T/SHRH 011-2018.

The principle method comprises the following steps: the chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chick embryo. Because the surface blood vessels of the chick embryo allantoic membrane are rich and can be regarded as a complete organism, the test utilizes the characteristics of complete, clear and transparent blood vessel system of chorioallantoic membrane in the middle stage of a hatched chick embryo, a certain amount of a test object is directly contacted with the chick embryo allantoic membrane, the change of chorioallantoic membrane toxicity effect indexes (such as bleeding, blood coagulation and blood vessel melting) is observed after the test object acts for a specified time, the score is given, and the mathematical average value is calculated for evaluating the eye irritation of the test object.

The detection method comprises the following steps: selecting fertilized chicken embryos of White Hangzhou chickens (White Leghorn chicken) and other varieties, incubating until the 8 th day, examining the eggs by an egg candler, discarding unfertilized and inactive chicken embryos, selecting chicken embryos with good blood vessel development, and marking the positions of air chambers on the surfaces of eggshells. The test was started on day 9. Carefully peeling off the eggshell part of the air chamber by dental bending, dripping a few drops of physiological saline on the surface of the eggshell membrane to fully wet the eggshell membrane, pouring out the eggshell membrane, and carefully removing the eggshell membrane by using a forceps to ensure that the exposed allantoic membrane is intact and does not suffer any damage. 0.3mL/0.3g of the test substance was applied directly to the CAM. Spread as much as possible to ensure coverage of at least 50%. After 3min of action, the CAM surface was gently rinsed with normal saline, the rinsing was completed within 30s, the liquid was decanted, the degree of change in each toxic effect was immediately observed under a stereomicroscope, and a score (ES) was given. Conditions allowed that pictures should be taken immediately after the 3min exposure was complete at the beginning of the observation.

The judgment basis is as follows: judged according to the score (ES). ES is less than or equal to 4 without stimulation, 4-Ap ES is less than or equal to 12 with mild stimulation, 12-Ap ES is less than or equal to 16 with moderate stimulation, and ES is more than or equal to 16 with severe stimulation.

The specific evaluation is shown in table 4 below:

TABLE 4

Each group of samples corresponds to 6 chick embryos, and the test result shows that ES of all test samples (the skin repair compositions provided by examples 1-19 and comparative examples 1-3) is less than or equal to 4 under the condition that the concentration of a test substance is 100 percent, so that the skin repair composition provided by the invention is safe and non-irritant.

The applicant states that the present invention is illustrated by the above examples of the process of the present invention, but the present invention is not limited to the above process steps, i.e. it is not meant that the present invention must rely on the above process steps to be carried out. It will be apparent to those skilled in the art that any modification of the present invention, equivalent substitutions of selected materials and additions of auxiliary components, selection of specific modes and the like, which are within the scope and disclosure of the present invention, are contemplated by the present invention.

Claims

1. A skin rejuvenation composition comprising an amoebosome, water and an active agent loaded into said amoebosome, said active agent comprising sodium DNA, nicotinamide adenine dinucleotide, ceramide and a natural product extract.

2. The skin rejuvenation composition as defined in claim 1 wherein said skin rejuvenation composition includes, in parts by weight, 11 to 27 parts of deformed vesicles, 2 to 54 parts of water, and 35 to 88 parts of active agents carried in said deformed vesicles;

preferably, the active substance comprises 0.5-1 part of DNA sodium, 0.5-1 part of nicotinamide adenine dinucleotide, 2.5-5 parts of ceramide and 17-40 parts of natural product extract in parts by weight.

3. The skin rejuvenation composition as defined in claim 1 or 2 wherein said natural product extract comprises any one or a combination of at least two of madecassoside, alpine edelweiss callus extract, hypecoum extract, thermus thermophilus fermentation product, gentian root extract, or matricaria flower extract, preferably a combination of madecassoside, alpine edelweiss callus extract, hypecoum extract, thermus thermophilus fermentation product, gentian root extract, and matricaria flower extract;

preferably, the natural product extract comprises 5-15 parts by weight of madecassoside, 5-15 parts by weight of alpine edelweiss callus extract, 0.5-1 part by weight of hypecoum extract, 1-3 parts by weight of thermophilic thermus fermentation product, 0.5-1 part by weight of gentian extract and 0.5-1 part by weight of chamomile flower extract.

4. The skin rejuvenation composition AS defined in any one of claims 1 to 3 wherein said ceramide comprises any one or a combination of at least two of ceramide NS/ceramide NG, ceramide NP, ceramide EOP, ceramide AS, or ceramide AP, preferably a combination of ceramide NS/ceramide NG, ceramide NP, ceramide EOP, ceramide AS, and ceramide AP;

preferably, the ceramide comprises 0.5-1 part of ceramide NS/ceramide NG, 0.5-1 part of ceramide NP, 0.5-1 part of ceramide EOP, 0.5-1 part of ceramide AS and 0.5-1 part of ceramide AP by weight.

5. The skin rejuvenation composition as claimed in any one of claims 1 to 4 wherein said active substance further includes an oil;

preferably, the oil comprises any one or a combination of at least two of olive fruit oil, argan tree kernel oil, squalane, shea butter or macadamia nut seed oil, preferably the combination of olive fruit oil, argan tree kernel oil, squalane, shea butter and macadamia nut seed oil;

preferably, the grease comprises, by weight, 5-10 parts of olive fruit oil, 5-10 parts of argania spinosa kernel oil, 5-10 parts of squalane, 1-5 parts of shea butter and 1-5 parts of macadamia nut seed oil;

preferably, the active substance further comprises 1, 2-hexanediol;

preferably, the active substance also comprises 2-5 parts of 1, 2-hexanediol according to parts by weight.

6. The skin rejuvenating composition as claimed in any one of claims 1 to 5 wherein the proteoliposomes are prepared from materials including PEG/PPG-14/7 dimethyl ether, polyglycerol ester surfactants, sorbitan ester surfactants, cetyl phosphate, honokiol and magnolol;

preferably, the raw materials for preparing the deformed vesicle comprise, by weight, 2-5 parts of PEG/PPG-14/7 dimethyl ether, 4-10 parts of polyglycerol ester surfactant, 2-5 parts of sorbitan ester surfactant, 1-3 parts of cetyl alcohol phosphate, 1-2 parts of honokiol and 1-2 parts of magnolol;

preferably, the polyglycerol ester surfactant comprises a polyglycerol-6 ester of wild almond oil and/or polyglycerol-10 oleate, preferably a combination of a polyglycerol-6 ester of wild almond oil and polyglycerol-10 oleate;

preferably, the sorbitan ester surfactant comprises sorbitan palmitate;

preferably, the mass ratio of the magnolol to the polyglycerol ester surfactant is 1 (2-10).

7. A method of preparing a skin rejuvenating composition as claimed in any one of claims 1 to 6, which comprises the steps of: mixing the deformed vesicle, water and the active substance, stirring and homogenizing to obtain the skin repair composition.

8. The method of claim 7, wherein the temperature of the mixing is 35-45 ℃;

preferably, the rotation speed of the stirring is 50-70rpm, and the time is 0.8-1.2h;

preferably, the homogenizing speed is 4500-5500rpm for 4-6min.

9. The method for producing an amoebosome according to claim 7 or 8, wherein the method for producing an amoebosome comprises the steps of:

(2) Mixing the bilayer obtained in the step (1), PEG/PPG-14/7 dimethyl ether, honokiol and magnolol, and stirring to obtain the deformed vesicle;

preferably, in step (1), the temperature of the mixing is 55-65 ℃;

preferably, in the step (1), the rotation speed of the stirring is 45-55rpm, and the time is 20-40min;

preferably, in the step (2), the temperature of the mixing is 40-50 ℃;

preferably, in the step (2), the rotation speed of the stirring is 35-45rpm, and the time is 20-40min.

10. Use of a skin repair composition according to any one of claims 1 to 6 in the preparation of a skin care product;

preferably, the skin care product comprises a cream and/or a serum.