CN102815793A - Blue-green alga ingestion method of Simocephalus vetulus - Google Patents
Blue-green alga ingestion method of Simocephalus vetulus Download PDFInfo
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- CN102815793A CN102815793A CN2012103080992A CN201210308099A CN102815793A CN 102815793 A CN102815793 A CN 102815793A CN 2012103080992 A CN2012103080992 A CN 2012103080992A CN 201210308099 A CN201210308099 A CN 201210308099A CN 102815793 A CN102815793 A CN 102815793A
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- 238000000034 method Methods 0.000 title claims abstract description 10
- 241001302205 Simocephalus vetulus Species 0.000 title abstract 6
- 230000037406 food intake Effects 0.000 title abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 87
- 241000238578 Daphnia Species 0.000 claims abstract description 43
- 241000192700 Cyanobacteria Species 0.000 claims abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 13
- 238000012851 eutrophication Methods 0.000 claims abstract description 13
- 241001465754 Metazoa Species 0.000 claims abstract description 7
- 238000009395 breeding Methods 0.000 claims abstract description 6
- 230000001488 breeding effect Effects 0.000 claims abstract description 6
- 229920001282 polysaccharide Polymers 0.000 claims description 19
- 239000005017 polysaccharide Substances 0.000 claims description 19
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- 235000019688 fish Nutrition 0.000 claims description 10
- 239000004677 Nylon Substances 0.000 claims description 9
- 229920001778 nylon Polymers 0.000 claims description 9
- 241001494246 Daphnia magna Species 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 241000252234 Hypophthalmichthys nobilis Species 0.000 claims description 5
- 238000003306 harvesting Methods 0.000 claims description 5
- 239000011148 porous material Substances 0.000 claims description 5
- 241000208340 Araliaceae Species 0.000 claims description 4
- 241001460967 Coilia nasus Species 0.000 claims description 4
- 229920001491 Lentinan Polymers 0.000 claims description 4
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 4
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 4
- 235000008434 ginseng Nutrition 0.000 claims description 4
- 229940115286 lentinan Drugs 0.000 claims description 4
- 239000002131 composite material Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 241000894007 species Species 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
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- 235000013305 food Nutrition 0.000 description 8
- 241000238571 Cladocera Species 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- 238000005273 aeration Methods 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
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- 241000755266 Kathetostoma giganteum Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 2
- 239000008239 natural water Substances 0.000 description 2
- 230000008186 parthenogenesis Effects 0.000 description 2
- -1 physics Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 235000015099 wheat brans Nutrition 0.000 description 2
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- 241000206761 Bacillariophyta Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000238423 Branchiopoda Species 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 241001430403 Coilia grayii Species 0.000 description 1
- 241000238576 Daphnia pulex Species 0.000 description 1
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- Cultivation Of Seaweed (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a blue-green alga ingestion method of Simocephalus vetulus. The method comprises: conducting enrichment and expansion cultivation and blue-green alga induction on Simocephalus vetulus collected in a natural eutrophic water body; selecting and breeding Simocephalus vetulus able to ingest blue-green algae and propagate normally; then according to the eutrophication degree of the water body needing control, putting a certain amount of adult Simocephalus vetulus into a unit of water body, and under the condition of no next-stage organism predation pressure, and making use of the adult Simocephalus vetulus to ingest microalgae, bacteria and organic detritus in the water body to increase the water body transparency rapidly and promote restoration of other animals and plants in the water body, thus finally achieving ecological balance of the water environment. The method disclosed in the invention has the characteristics of easily available materials, convenient cultivation, low cost, environmental safety of aboriginal species, high efficiency and the like, thus providing a new daphnia species for the technology of biological control of water eutrophication.
Description
Technical field
The invention belongs to the field of biological control of water pollution, in particular to lower the amount of domesticated elderly cyanobacteria Daphnia feeding method.
Background technology
After biology in the natural water (producer, human consumer, decomposer) composition reached a kind of rational balance, the water body diversity of organism just can better embody, and it is better that the self-purification capacity of water body could more be brought into play.Along with the influence of the mankind to physical environment; Water body eutrophication degree is increasingly high a few days ago; Cause that miniature planktonic algae breeds in a large number in the water body, particularly harmful algae (being commonly called as " water bloom " or " wawter bloom ") such as micro-capsule blue-green algae breaks out in summer, autumn (high temperature) water body, and frond after death can discharge toxin, water hypoxia stench, make the hydrocoles mass mortality; Water body environment extremely worsens, and causes the sight of water body and utility value to reduce greatly.
The method of administering body eutrophication at present is a lot, like desilting, and allotment water, physics, chemical algae removing and input bacterium (as dissolving phycomycete) and biological regulation and control etc. have all obtained certain effect.
The food chain of applying biological (the non-classical bio operon technology of Jing Dian is arranged) is being obtained interim progress aspect the body eutrophication: under the guidance of back Liu Jiankang academician of studying abroad and comeing back like doctor Xie Ping of Inst. of Hydrobiology, Chinese Academy of Sciences;, nineteen ninety in 1989 and 1992 enclosure test through being located in the lake for three times; Putting in a suitable place to breed in a large number of proof silver carp, flathead is the deciding factor that wawter bloom disappears.Find the Wuhan East Lake reason----" opening the mystery of Wuhan East Lake blue-green alga bloom disappearance " that blue-green algae no longer breaks out over nearly 14 years 1999.Because filter-feeding fish is had relatively high expectations to the dissolved oxygen of water body, can well existence under low dissolved oxygen condition so seek a kind of biology, the biology----cladocera of the wawter bloom of ingesting again becomes the focus of everybody research.
Cladocera belongs to Arthropoda, Crustachia, Branchiopoda, two Leptostraca, branch angle suborder, and the common name water flea is commonly called as " red worm " or " worm for fishing ".Its life of in water body, swimming is a food with little algae (like chlorella, grid algae etc.), bacterium (like yeast, genus bacillus etc.), organic detritus (like the wheat bran behind the soaking fermentation, rice bran etc.), and it is an important bait of fish seed stage.Currently in China's control of water Cladocera declare the patent is Daphnia magna, Daphnia pulex, Daphnia and tip amount Moina.The present inventors conducted in Chuzhou City, Anhui eutrophic water sampling accidentally discovered Daphnia elderly low amount, according to Professor Zhao Wen 2008 editor of "brine Cladocera Biology and seawater cultured using the" literature shows:
(1) China does not find temporarily except that Anhui, Inner Mongol and Ningxia and does not have the report, and all the other each provinces and regions are all on the books, and abroad except that Oceania, it is distributed widely in all over the world;
(2) eurythermic.Happiness is lived in the dense bed mud of pasture and water darker the loke shore limit and pond.Usually utilize its swimming bristle to be attached on the pasture and water, population occurs early spring, and disappear early winter;
(3) can carry out parthenogenesis and gamogenesis replaces, breeding is very fast during parthenogenesis.In large-scale waters, carry out the monocycle reproduction, in little water body, carry out the multicycle reproduction.Summer egg output is very big, and spring, every tire can reach 20-35.Summer and autumn, every tire quantity was compared less;
(4) anti-low dissolved oxygen can be survived in the oxygen free condition environment 5 hours.
Summary of the invention
The purpose of the present invention is to provide a low amount of elderly cyanobacteria Daphnia feeding methods, collecting eutrophic water in natural conditions and induced to lower the amount of elderly survive Daphnia (water cyanobacteria are often able to maintain a longer period of time? <? such as the occurrence of Chaohu Lake Dian long duration, can be seen from April to November each year, even in December, but also occasionally chiffon silk-like blue-green indigo lake leaf floating on the lake look --- Ming poetry, etc., 1981 ?>, this long period of time sufficient to cause the change of zooplankton genotypes better adapted to changes in the environment.< this phenomenon in the research of the lake of Germany, be confirmed----Hairston et al.1999 >); It is carried out " mono-clonal " enlarged culturing; After the hot housing of wet blue-green algae before the application in the also available natural eutrophication water; Be used for the biology regulation and control of eutrophication water, in the hope of reaching the purpose of administering the eutrophic water body environmental pollution.
For realizing above-mentioned purpose, the present invention adopts following technical scheme:
Lower the amount of cyanobacteria old Daphnia feeding method comprising: collecting eutrophic water in natural conditions and induced to lower the amount of elderly survive Daphnia (eg eutrophication of lakes, reservoirs, rivers, ponds, ditches and other water bodies lower the amount of old Daphnia), inoculated into rich microalgae, bacteria, organic detritus pool in a "monoclonal" expanding culture, with the pore size of about 1.0 × 1.0mm nylon sieve (strainer) cultured in expanding elderly low amount magna graded harvest: mesh larvae animal is still less than the original water continued to train; Pipette the appropriate amount into the old low amount Daphnia magna (not through nylon sieve into a large-sized body) to contain natural water bodies to further strengthen the culture of cyanobacteria.After breeding the enhanced low-normal amount of reproductive age Daphnia further expanding culture; final treatment unit can be eutrophication degree, in the absence of a post-biological (eg: lake anchovy, bighead carp and other fish) predation put under pressure after a certain number of elderly strengthen lower the amount of Daphnia, using its right eutrophic water microalgae, bacteria, organic detritus feeding use, after some time, the water can significantly improve the transparency to promote other water animals and plants could be repaired, and ultimately achieve the ecological balance of the aquatic environment.
The rich microalgae, bacteria, organic detritus pool complex polysaccharide is added, the total dosage of water births 1-2, the lower the amount of old age can promote growth and reproduction of Daphnia; said composite polysaccharide as lentinan, LBP, ginseng polysaccharides, three kinds of polysaccharide weight ratio 1:1-2:1-2.
The present invention has following advantage:
A biological control lower the amount of material --- old Daphnia species get convenient, lower the amount of Daphnia as elderly native species invasion on the environment will not cause biological hazards, safety;
2, the lower the amount of old Daphnia mass culture is simple, you can use microalgae, bacteria, organic detritus, complex polysaccharides can achieve large-scale cultivation;
3, it is suitable for extensively envrionment temperature, and all ability normal growth, breedings are with low cost under 10-30 ℃ of condition;
4, it is used simple reinforcement of blue-green algae in the eutrophication water after; Under the condition of the pressure that one-level without issue biological (as: fish such as lake long-tailed anchovy, flathead) is preyed on; Be used for the biology regulation and control of body eutrophication water body; Can improve the transparency of water body rapidly,, better improve water body self-purification ability for aquatic macrophyte and other biological growth provide better living environment.
Embodiment
Further specify through specific embodiment below, embodiment only is used for the present invention and is not used in restriction scope of the present invention.
Embodiment 1
To lower the amount collected elderly Daphnia placed in the room filled with 120L white plastic bucket of water, the water needs aeration two days; irregular feeding 10-50? G organic detritus (soaked fermented wheat bran) to foods Daphnia elderly low rates for expanding culture; pending Daphnia biomass reaches 200 / l in the water after adding 5-10g wet algae to be strengthened induced acclimation to adapt to cyanobacteria as the main food of the water environment.
The domestication of elderly with low rates of Daphnia pore size of about 1.0 × 1.0mm nylon sieve (strainer) in elderly low rates for harvest Daphnia: Daphnia fine mesh smaller than in the original water continued to train; in the laboratory 0.1M
3 lower the amount of water added to the elderly as Daphnia magna (not through nylon sieve into a large-sized body) wet weight of 20 g; in the absence of predation pressure after a biological condition, using its right eutrophic water microalgae, bacteria, organic detritus feeding use 3-5 - 5 days after the water transparency increased rapidly from 10cm to 20cm, promote other water animals and plants repair, and ultimately achieve the ecological water environment improved.
Embodiment 2
To lower the amount collected elderly Daphnia placed in the room filled with 120L white plastic bucket of water, the water needs aeration two days; irregular feeding 5-10g baker's yeast, algae as a food for the elderly low rates magna expanding culture; subject to Daphnia biomass reaches 2? 00 / l in the water after adding 5-10g wet cyanobacteria (natural eutrophic water fish), water weight millionth compound polysaccharides, complex polysaccharides by the lentinan, LBP , ginseng polysaccharide mixture of the three polysaccharide weight ratio of 1:? 2:1, to be strengthened induced acclimation to adapt to cyanobacteria as the main food of the water environment, the vitality of older low amount of Daphnia, quantity, size, significantly higher than the Do not add water body complex polysaccharides.
The domestication of elderly with low rates of Daphnia pore size of about 1.0 × 1.0mm nylon sieve (strainer) in elderly low rates for harvest Daphnia: Daphnia fine mesh smaller than in the original water continued to train; in the laboratory 0.1M
3 lower the amount of water added to the elderly as Daphnia magna (not through nylon sieve into a large-sized body) wet weight of 30 g; without after a biological (eg: lake anchovy, bighead carp and other fish ) predation pressure conditions, using its eutrophic water microalgae algae), using its right eutrophic water microalgae, bacteria, organic detritus feeding use 3-5 - 5 days after the body of water the transparency of the rapid increase from 10cm to 30cm, promote other water animals and plants repair, and ultimately achieve the ecological water environment can be improved.
Embodiment 3
To lower the amount collected elderly Daphnia placed in the room filled with 120L white plastic bucket of water, the water needs aeration two days; irregular feeding 5-20? G microalgae (fish with a dense mesh of green manure pit pond algae, diatoms) for the low amount of food on the elderly for expanding culture of Daphnia, water added weight of the water millionth complex polysaccharides, complex polysaccharides by the lentinan, LBP, ginseng polysaccharide mixture of the three weight ratio of polysaccharide 1:? 2:1, older Daphnia low amount of energy, number, size, significantly higher than that without adding complex polysaccharides body of water; pending Daphnia biomass reaches 200 / l in the water after adding 5-10g wet cyanobacteria (Natural Wealth eutrophic water fish) to induce acclimation to adapt to cyanobacteria as the main food of the water environment.
The domestication of elderly with low rates of Daphnia pore size of about 1.0 × 1.0mm nylon sieve (strainer) in elderly low rates for harvest Daphnia: Daphnia fine mesh smaller than in the original water continued to train; in the laboratory 0.1M
3 lower the amount of water added to the elderly as Daphnia magna (not through nylon sieve into a large-sized body) wet weight of 40 g; without after a biological (eg: lake anchovy, bighead carp and other fish ) predation pressure conditions, using its eutrophic water microalgae (blue-green algae), using its right eutrophic water microalgae, bacteria, organic detritus feeding use, five days after the water transparency increased rapidly from 10cm to 50cm, promote other water animals and plants, repair, and ultimately achieve the ecological water environment can be improved.
Claims (2)
1 older low rates cyanobacteria Daphnia feeding method comprising: collecting old Eutrophication lower the amount of Daphnia (eg eutrophication of lakes, reservoirs, rivers, ponds, canals and other water bodies) were inoculated into rich microalgae, bacteria, organic detritus pool in a "monoclonal" expanding culture; with pore size of about 1.0 × 1.0mm nylon sieve (strainer) cultured on expanding the amount of older low graded on Daphnia harvest: less than mesh baby Daphnia in the original water continued to train; Pipette the appropriate amount of large-sized amount of older low Daphnia magna into water containing cyanobacteria further strengthen the culture; breeding by the strengthened lower the amount of the normal breeding age for expanding culture of Daphnia; final according to Subject to eutrophication management unit level, in the absence of a post-biological (eg: lake anchovy, bighead carp and other fish) predation pressure conditions lower the amount put in a certain number of elderly Daphnia, using its right of eutrophic water microalgae, bacteria, the use of organic detritus feeding, after some time, the water can significantly improve the transparency, in order to promote other water animals and plants can be repaired, and ultimately to the ecological balance of the water environment.
(2) as claimed in claim 1, wherein the lower the amount of old Daphnia feeding cyanobacteria, characterized in that: said rich microalgae, bacteria, organic detritus pool complex polysaccharide is added, the total addition amount of the water extremely The 1-2, promote elderly low amount Daphnia growth and reproduction; said composite polysaccharide lentinan, LBP, ginseng polysaccharides, three kinds of polysaccharide weight ratio 1:1-2:1-2.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105036348A (en) * | 2015-07-29 | 2015-11-11 | 苏州翠浔水生态科技有限公司 | Method for controlling blue-green algae in shallow water eutrophication lakes through utilizing large zooplanktons |
| CN114568284A (en) * | 2022-02-21 | 2022-06-03 | 中国科学院海洋研究所 | Biological coupling culture system and method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1884138A (en) * | 2005-06-21 | 2006-12-27 | 云南正清生态技术有限公司 | Method for domesticating daphnia pulex, A. diaphana King, or moina irrasa in lake to eat blue algae |
| CN101575144A (en) * | 2008-05-07 | 2009-11-11 | 何文辉 | Method for repairing water ecology |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1884138A (en) * | 2005-06-21 | 2006-12-27 | 云南正清生态技术有限公司 | Method for domesticating daphnia pulex, A. diaphana King, or moina irrasa in lake to eat blue algae |
| CN101575144A (en) * | 2008-05-07 | 2009-11-11 | 何文辉 | Method for repairing water ecology |
Non-Patent Citations (1)
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| 张跃伟: "白洋淀控藻生物链研究", 《CNKI优秀硕士学位论文全文数据库》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105036348A (en) * | 2015-07-29 | 2015-11-11 | 苏州翠浔水生态科技有限公司 | Method for controlling blue-green algae in shallow water eutrophication lakes through utilizing large zooplanktons |
| CN114568284A (en) * | 2022-02-21 | 2022-06-03 | 中国科学院海洋研究所 | Biological coupling culture system and method thereof |
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Effective date of registration: 20170829 Address after: 239056 Anhui city in Chuzhou Province, Nanqiao District Nanqiao Road Patentee after: Anhui union education Limited Address before: 239000 No. 99 West Feng Feng Road, Anhui, Chuzhou (room 8018 of people's Congress Hotel) Patentee before: Anhui Lianda Biological Environmental Protection Technology Co., Ltd. |
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