CN101897304A - A culture method for improving the survival rate and nucleus retention rate of Pinctada martensii during the recuperation period - Google Patents

Abstract

The invention relates to a cultivation method for improving the survival rate and the nucleus-remaining rate of nucleus-inserted Pinctada martensii during a recuperation period, comprising the following steps: selecting a pond of 100-500 mu, wherein the dike height thereof is 4.0-5.0m, the water depth is 2.5-3.5m, the bottom of the pond is provided with a silt bottom material, and water can be conveniently charged into or discharged out of the pond; utilizing the pond to cultivate Tetraselmis, chlorella or chlorella sp and other Chlamydomonas 7-10 days before a nucleus inserting operation; selecting individuals with strong activity, the age of 1.5-2.0 and the shell length of 6-7cm to carry out the nucleus inserting operation; after finishing the nucleus inserting operation, cultivating the nucleus-inserted Pinctada martensii in the pond in a hanging mode, wherein the cultivation density is 1500-2000 pieces per mu; and recuperating for one month to obtain the nucleus-inserted Pinctada martensii with the survival rate and the nucleus-remaining rate of above 80% and 50% respectively. The invention has simple, convenient and feasible operation, can obviously improve the survival rate and the nucleus-remaining rate of the nucleus-inserted Pinctada martensii and improves the quality and yield of pearls.

Description

A culture method for improving the survival rate and nucleus retention rate of Pinctada martensii during the recuperation period

technical field

The invention belongs to the field of shellfish culture and provides a culture method for improving the survival rate and nucleus retention rate of Pinctada martensii during the recuperation period.

Background technique

Pinctada martensii is distributed in the coastal areas of Guangdong, Guangxi and Hainan in my country, and is one of the important shellfish for cultivating seawater pearls. Since the successful artificial breeding of Pinctada martensii in my country in 1965, seawater pearl cultivation has developed rapidly. Especially in the 1990s, seawater pearl cultivation has become a pillar industry in coastal areas of Guangdong, Guangxi and Hainan. In the 1990s, my country's seawater pearl cultivation area was nearly 200,000 mu, with an annual output of about 30 tons of pearls, and the output value of unprocessed primary products reached about 1 billion yuan. However, in recent years, although the output of seawater pearls has increased, the quality has declined significantly, and the phenomenon of "high yield and low value" has appeared. Therefore, it is very urgent to systematically carry out research on seawater pearl breeding technology.

Postoperative recuperation is an important part of pearl cultivation. After the core implantation operation, the core implanted oysters are severely damaged and weak, and generally need 10-30 days to recuperate. The purpose of recuperation, on the one hand, is to restore health to the operated oyster, gradually restore the physiological activities from the suppressed state to the normal state, and prevent the operation oyster from dying; Denucleation, production of plain beads and deformed beads (Xie Zhongming, 2004). The recuperation site generally chooses a sea area with flat wind, calm waves, good water quality and few harmful organisms. If the harsh sea environment conditions affect the recovery of surgical shellfish wounds, the survival rate and nucleus retention rate of surgical shellfish will be reduced.

Contents of the invention

The purpose of the present invention is to provide a kind of culture method that improves the survival rate and nucleus retention rate of Pinctada martensii in the recuperation period, and solves the problem that in seawater pearl cultivation, after the nucleus insertion operation, the nucleus insert has been severely damaged. Injury, physical weakness, need to recuperate, so that the operation shell recovers health, physiological activities gradually return from the suppressed state to normal state, prevent the operation shell death; make the bead nuclei do not move before the cell flakes form pearl sacs, and prevent denucleation , The problems of plain beads and deformed beads.

In order to achieve the above-mentioned purpose, the technical scheme that the present invention adopts is: 1) select 100-500 mu of ponds for use, embankment height 4.0-5.0m, depth of water 2.5-3.5m, bottom silt bottom quality of the pond, inflow and drainage are convenient; 2) insert 7-10 days before the nuclear operation, cultivate flat algae, chlorella or cloud microalgae Monocystis in the pond; 3) select individuals with strong vitality, shellfish age 1.5-2.0 years old, and shell length 6-7cm for nuclear insertion Operation; 4) After the core insertion operation, the operated shellfish is suspended and raised in the pond, and the breeding density is 1500-2000/mu. The breeding management is carried out according to the conventional technology. After the one-month rest period is over, the shellfish are cleaned up, and the survival rate and the rate of nuclear retention are counted.

The present invention has the following advantages: the use of ponds to cultivate the oysters can provide abundant biological bait, accelerate the wound healing of the oysters and the formation of pearl sacs; remarkably improve the survival rate and nucleus retention rate of the oysters, and increase the pearl output and quality.

Detailed ways

Example 1

Location: Zhanjiang Xuwen Dajing Anhua Group Co., Ltd.; Time: April-June 2007. A pond of 492 mu is used, the dike height is 4.0m, the water depth is 3.0m, and the bottom of the pond is silt. Ten days before the implantation operation, the cloud microalgae bait in the outdoor cement pool was transferred to the pond. After 5-7 days, the cloud microalgae became the dominant algae in the pond water body. The cultured population of Pinctada martensii in Xuwenchengwu was used to perform enucleation surgery. The age of shellfish was 1.5-2.0 years old, the shell length was 6.0-7.0cm, and the number of enculturation shellfish was 1 million. After the implantation operation, 500,000 implanted shellfish will be raised in ponds and 500,000 in natural sea areas. After the 1-month recuperation period, the survival rate and nuclear retention rate of pearl oysters were 88.4±7.6% and 65.2±5.9%, respectively, which were 21.9% and 14.1% higher than those of the control group in natural sea areas.

Example 2

Location: Zhanjiang Xuwen Dajing Anhua Group Co., Ltd.; Time: September-October 2008. A pond of 492 mu is used, the dike height is 4.0m, the water depth is 3.0m, and the bottom of the pond is silt. Seven days before the core insertion operation, the chlorella and flat algae in the outdoor cement pool were transferred to the pond. After 7 days of cultivation, the chlorella and flat algae became the dominant algae in the pond water. Using Xuwen Chengwu and Leizhou Houhong martensii breeding populations to perform enucleation surgery, the shellfish age is 2.0 years old, the shell length is 6.0-7.5cm, and the number of engrafted oysters is 2 million. After the implantation operation, 800,000 implanted shellfish were suspended in ponds and 1.2 million in natural sea areas. After the 1-month recuperation period, the survival rate and nuclear retention rate of pearl oysters were 85.7±9.2% and 62.1±7.8%, respectively, which were 25.4% and 11.7% higher than those of the control group in the natural sea area.

Claims (1)

1. one kind is improved the cultural method that pteria martensii is inserted nuclear shellfish during recuperation period survival rate and core-remaining rate, it is characterized in that comprising the steps:

1) select 100-500 mu pond for use, the high 4.0-5.0m of dike, depth of water 2.5-3.5m, bed mud husky substrate in pond advances draining conveniently;

2) insert front 7-10 days of nuclear operation, utilize the algae such as pond culture flat algae, chlorella or Yun Weizao;

3) select for use vigor stronger, shellfish 1.5-2.0 in age age, the individuality of the long 6-7cm of shell is inserted the nuclear operation;

4) insert the nuclear operation after, the shellfish hanging culture of will perform the operation is in the pond, cultivation density 1500-2000 only/mu.