CN102786030B - Method for preparing polypeptide nano-film by solvent treatment - Google Patents
Method for preparing polypeptide nano-film by solvent treatment Download PDFInfo
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- CN102786030B CN102786030B CN201210233011.5A CN201210233011A CN102786030B CN 102786030 B CN102786030 B CN 102786030B CN 201210233011 A CN201210233011 A CN 201210233011A CN 102786030 B CN102786030 B CN 102786030B
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- polypeptide
- dipeptide
- film
- nanofilm
- polypeptide nano
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 51
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 46
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 46
- 239000002120 nanofilm Substances 0.000 title claims abstract description 38
- 239000002904 solvent Substances 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 14
- 108010016626 Dipeptides Proteins 0.000 claims abstract description 14
- DMLAVOWQYNRWNQ-UHFFFAOYSA-N azobenzene Chemical compound C1=CC=CC=C1N=NC1=CC=CC=C1 DMLAVOWQYNRWNQ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000002121 nanofiber Substances 0.000 claims abstract description 5
- 238000004528 spin coating Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000000758 substrate Substances 0.000 claims abstract 4
- 238000001035 drying Methods 0.000 claims abstract 3
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims description 13
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 12
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 5
- BYEAHWXPCBROCE-UHFFFAOYSA-N 1,1,1,3,3,3-hexafluoropropan-2-ol Chemical compound FC(F)(F)C(O)C(F)(F)F BYEAHWXPCBROCE-UHFFFAOYSA-N 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 239000010445 mica Substances 0.000 claims description 4
- 229910052618 mica group Inorganic materials 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 239000012265 solid product Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000002243 precursor Substances 0.000 claims 2
- 238000005227 gel permeation chromatography Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000005507 spraying Methods 0.000 claims 1
- 239000012620 biological material Substances 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 230000003287 optical effect Effects 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 102000004860 Dipeptidases Human genes 0.000 description 5
- 108090001081 Dipeptidases Proteins 0.000 description 5
- 241001597008 Nomeidae Species 0.000 description 5
- 239000010408 film Substances 0.000 description 4
- 238000009736 wetting Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 238000007385 chemical modification Methods 0.000 description 3
- 230000006837 decompression Effects 0.000 description 3
- 238000004821 distillation Methods 0.000 description 3
- 239000002086 nanomaterial Substances 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 125000003118 aryl group Chemical group 0.000 description 2
- 238000000089 atomic force micrograph Methods 0.000 description 2
- 238000005229 chemical vapour deposition Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002071 nanotube Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
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Abstract
本发明一种通过溶剂处理制备多肽纳米薄膜的方法，属于生物材料领域。该方法包括如下步骤：对二肽分子进行化学修饰，连接偶氮苯基团；将修饰后的二肽分子溶解于良溶剂，旋涂于亲水基底；用水处理表面，干燥后得到多肽纳米薄膜。本发明所用方法操作简单，成本相对低廉，很容易大面积制备，解决了从多肽纳米纤维制备纳米薄膜的关键问题。所得到的多肽纳米薄膜在纳米尺度上结构规整有序，有望用于组织工程材料，生物相容性表面修饰，基于偶氮苯的光存储薄膜等领域。
The invention relates to a method for preparing a polypeptide nano film by solvent treatment, which belongs to the field of biological materials. The method comprises the following steps: chemically modifying the dipeptide molecule and connecting an azophenyl group; dissolving the modified dipeptide molecule in a good solvent, and spin-coating it on a hydrophilic substrate; treating the surface with water, and drying to obtain a polypeptide nano-film . The method used in the invention is simple in operation, relatively low in cost, easy to prepare in a large area, and solves the key problem of preparing nano film from polypeptide nano fiber. The obtained polypeptide nanofilm has a regular and orderly structure at the nanometer scale, and is expected to be used in tissue engineering materials, biocompatible surface modification, azobenzene-based optical storage films and other fields.
Description
Technical field
The invention belongs to technical field of biological material.Specifically, the present invention relates to a kind of new method of preparing polypeptide nano film of processing by solvent.
Background technology
As a kind of novel biomaterial, polypeptide nano material has many advantages, for example: good biocompatibility, chemical modification be simple directly, possess biomolecule recognition capability.Two peptide molecules are due to simple in structure, and the feature that cost is relatively cheap, becomes a kind of ideal of constructing preparation nano material and construct primitive.Before, it is found that aromatic series dipeptides is easy to by solvent evaporates (Nat Nano 2006,1 (3), 195) or chemical vapour deposition (CVD) (Nat Nano 2009,4 (12), 849) method is assembled into large-area two-dimensional nano pipe array.Prepared nanotube has good chemistry and physical stability, and satisfactory mechanical property is a kind of nano material of excellence.
The present invention is by carrying out chemical modification to two peptide molecules, the method of processing in conjunction with solvent, obtained large-arealy by the fibrous polypeptide nano film of polypeptide nano, on nanoscale, having realized the control to polypeptide nano membrane structure, had broad application prospects.
Summary of the invention
The object of the invention is to, a kind of new method of preparing polypeptide nano film is provided.By solvent, process a method of preparing polypeptide nano film, according to following step, carry out:
1) two peptide molecules are carried out to chemical modification, connect azobenzene group;
2) by the dipeptides molecular melting after modifying in good solvent, at the bottom of being spun on hydrophilic group;
3) water treatment surface, obtains polypeptide nano film after being dried.
In wherein said step 1), specifically carry out in accordance with the following steps:
I) described two peptide molecules are dissolved in to the solution that anhydrous tetrahydro furan obtains 5 mmol/L;
Ii) to step I) add azobenzene-4-chlorobenzoyl chloride and the triethylamine of 1 times in the solution that obtains, more than stirring 2 h, to filter, filtrate decompression distillation obtains solid product; Product is purified and is obtained dipeptidase derivant with gel chromatographic columns, and drip washing solvent is oxolane.
In wherein said step 1), preferably described two peptide molecules are aromatic series dipeptides two L-Phes.
Wherein said step 2), in, specifically carry out in accordance with the following steps:
I) described dipeptidase derivant is dissolved in to good solvent with 0.1-1 mmol/L and obtains dipeptidase derivant solution;
Ii) by described dipeptidase derivant solution with rotating speeds more than 500 rpm more than hydrophilic group basal surface spin coating 1 min, obtain polypeptide nano film presoma.
Wherein said step 2) in, preferred described good solvent is hexafluoroisopropanol, is mica at the bottom of described hydrophilic group.
In wherein said step 3), specifically carry out in accordance with the following steps:
I) use atomizer spray on described polypeptide nano film presoma, obtain wetting polypeptide nano film;
Ii) described wetting polypeptide nano film is dry at normal temperatures, obtain by the fibrous polypeptide nano film of polypeptide nano.
The present invention at least has following meaning:
The present invention utilizes method simply and effectively to prepare polypeptide nano film, and simple to operate, cost is relatively cheap, is easy to large area preparation, has solved the key issue of preparing nano thin-film from polypeptide nano fiber.The resulting polypeptide nano film of the present invention compound with regular structure on nanoscale is orderly, is expected to for tissue engineering material, and biocompatible surfaces is modified, the fields such as the optical storage film of azo-based benzene.
Accompanying drawing explanation
Fig. 1 is the atomic force microscope images of the polypeptide nano film prepared from 1 mmol/L solution.The large figure of 50 * 50 μ m on the left side shows film homogeneous within the scope of large area, and 10 * 10 μ m full resolution prictures on the right show that film reaches tens of micron/nano fiber alignment by many and forms, every wide approximately 40 nm of nanofiber, high approximately 4 nm.
Fig. 2 is the atomic force microscope images of the polypeptide nano film prepared from 0.1 mmol/L solution.Film demonstrates similar structure.
The specific embodiment
The present invention is described in further detail as example to take optimum condition below.
, the material that uses chemical constitution
Two L-Phes (PP, described dipeptides), azobenzene-4-chlorobenzoyl chloride, the chemical constitution of the azobenzene derivatives of two L-Phes (PPA, described dipeptidase derivant) is as follows:
Two L-Phes (PP)
Azobenzene-4-chlorobenzoyl chloride
The azobenzene derivatives of two L-Phes (PPA)
embodiment 1
PPA is dissolved in to the solution that anhydrous tetrahydro furan obtains 5 mmol/L, adds azobenzene-4-chlorobenzoyl chloride and the triethylamine of 1 times, stir 2 h, filter, filtrate decompression distillation obtains solid product.Product is purified and is obtained PPA with gel chromatographic columns, and drip washing solvent is oxolane.
PPA is dissolved in to hexafluoroisopropanol with 1 mmol/L and obtains PPA solution, with the rotating speed of 3000 rpm, at mica surface spin coating 2 min, obtain polypeptide nano film presoma.With atomizer spray, on described polypeptide nano film presoma, obtain wetting polypeptide nano film, dry at normal temperatures, obtain by the fibrous polypeptide nano film of polypeptide nano.
Utilize commercial multiple mode scanning probe microscope (SPM, Nanoscope V, Veeco company, the U.S.), experiment condition is to rap pattern under atmosphere, and polypeptide nano film is scanned, and obtains high-resolution afm image, as shown in Figure 1.
embodiment 2
PPA is dissolved in to the solution that anhydrous tetrahydro furan obtains 5 mmol/L, adds azobenzene-4-chlorobenzoyl chloride and the triethylamine of 1 times, stir 2 h, filter, filtrate decompression distillation obtains solid product.Product is purified and is obtained PPA with gel chromatographic columns, and drip washing solvent is oxolane.
PPA is dissolved in to hexafluoroisopropanol with 0.1 mmol/L and obtains PPA solution, with the rotating speed of 3000 rpm, at mica surface spin coating 2 min, obtain polypeptide nano film presoma.With atomizer spray, on described polypeptide nano film presoma, obtain wetting polypeptide nano film, dry at normal temperatures, obtain by the fibrous polypeptide nano film of polypeptide nano.
Utilize commercial multiple mode scanning probe microscope (SPM, Nanoscope V, Veeco company, the U.S.), experiment condition is to rap pattern under atmosphere, and polypeptide nano film is scanned, and obtains high-resolution afm image, as shown in Figure 2.
Claims (2)
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| CN111671971B (en) * | 2020-07-30 | 2022-01-25 | 齐鲁工业大学 | Polypeptide single-layer film with 6% primary amino group exposure and preparation method and application thereof |
| CN114437171A (en) * | 2022-02-23 | 2022-05-06 | 北京工商大学 | Dipeptide surfactant containing azobenzene group and preparation method thereof |
| CN114605290B (en) * | 2022-03-03 | 2023-04-07 | 浙江大学杭州国际科创中心 | Amino acid spiral array film and preparation method thereof |
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| CN1662815A (en) * | 2002-04-22 | 2005-08-31 | 佛罗里达州立大学 | Functionalized nanoparticles and methods of use thereof |
| CN101973513A (en) * | 2010-08-31 | 2011-02-16 | 电子科技大学 | Method for preparing silicon-oxide-based semiconductor nano-film |
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| US7666661B2 (en) * | 2001-08-27 | 2010-02-23 | Platypus Technologies, Llc | Substrates, devices, and methods for quantitative liquid crystal assays |
| MXPA04003333A (en) * | 2001-10-10 | 2006-02-22 | Neose Technologies Inc | Remodeling and glycoconjugation of peptides. |
| KR20030084279A (en) * | 2002-04-26 | 2003-11-01 | 이진규 | A method for preparing large-area materials with well-defined nanostructure using nanoporous alumina or nano-textured aluminum |
| US8114843B2 (en) * | 2005-11-18 | 2012-02-14 | The Regents Of The University Of California | Photoreactive regulator of protein function and methods of use thereof |
| FR2951449B1 (en) * | 2009-10-15 | 2011-11-25 | Commissariat Energie Atomique | METHOD FOR FUNCTIONALIZATION OF BIOLOGICAL MOLECULES |
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| CN1662815A (en) * | 2002-04-22 | 2005-08-31 | 佛罗里达州立大学 | Functionalized nanoparticles and methods of use thereof |
| CN101973513A (en) * | 2010-08-31 | 2011-02-16 | 电子科技大学 | Method for preparing silicon-oxide-based semiconductor nano-film |
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