CN102743240A - Method and device for performing artificial insemination on cows - Google Patents
Method and device for performing artificial insemination on cows Download PDFInfo
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- CN102743240A CN102743240A CN2012102803509A CN201210280350A CN102743240A CN 102743240 A CN102743240 A CN 102743240A CN 2012102803509 A CN2012102803509 A CN 2012102803509A CN 201210280350 A CN201210280350 A CN 201210280350A CN 102743240 A CN102743240 A CN 102743240A
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- 241000283690 Bos taurus Species 0.000 title claims abstract description 86
- 230000009027 insemination Effects 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 16
- 210000000582 semen Anatomy 0.000 claims abstract description 43
- 230000008021 deposition Effects 0.000 claims abstract description 28
- 238000004140 cleaning Methods 0.000 claims abstract description 24
- 210000001215 vagina Anatomy 0.000 claims abstract description 21
- 210000000664 rectum Anatomy 0.000 claims abstract description 12
- 210000003679 cervix uteri Anatomy 0.000 claims abstract description 11
- 230000000249 desinfective effect Effects 0.000 claims abstract description 6
- 239000004743 Polypropylene Substances 0.000 claims description 19
- -1 polypropylene Polymers 0.000 claims description 19
- 229920001155 polypropylene Polymers 0.000 claims description 19
- 210000005239 tubule Anatomy 0.000 claims description 14
- 230000012173 estrus Effects 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 239000010902 straw Substances 0.000 claims description 11
- 238000013459 approach Methods 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 238000010257 thawing Methods 0.000 claims description 5
- 230000000844 anti-bacterial effect Effects 0.000 claims description 4
- 125000001246 bromo group Chemical group Br* 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000011156 evaluation Methods 0.000 claims description 4
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 230000000007 visual effect Effects 0.000 claims description 4
- 230000002421 anti-septic effect Effects 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 238000004806 packaging method and process Methods 0.000 claims description 3
- 239000002985 plastic film Substances 0.000 claims description 3
- 229920006255 plastic film Polymers 0.000 claims description 3
- 230000028327 secretion Effects 0.000 claims description 3
- 230000005855 radiation Effects 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 6
- 210000004291 uterus Anatomy 0.000 abstract description 6
- 208000017443 reproductive system disease Diseases 0.000 abstract description 2
- 238000011109 contamination Methods 0.000 abstract 5
- 210000003905 vulva Anatomy 0.000 abstract 2
- 230000006378 damage Effects 0.000 description 5
- 239000000645 desinfectant Substances 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 206010025482 malaise Diseases 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 230000016087 ovulation Effects 0.000 description 2
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- 208000019901 Anxiety disease Diseases 0.000 description 1
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- 229940088710 antibiotic agent Drugs 0.000 description 1
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Abstract
The invention discloses a method and a device for performing artificial insemination on cows. An anti-contamination outer sleeve is inserted into the deep of a cow vagina before a semen deposition gun is inserted into the cow vagina. One hand is deeply inserted into the rectum to perform artificial insemination operation. After the hand in the rectum catches the cervix uterus, the other hand holds the semen deposition gun and enables the head of the semen deposition gun to be inserted into the cow cervix uterus to perform semen deposition through a semen deposition gun inserting hole of the anti-contamination outer sleeve, so that the step of cleaning and disinfecting the cow vulva is omitted, and contamination of the semen deposition gun is avoided. According to the method and the device for performing the artificial insemination on the cows, the artificial insemination technology is improved scientifically in an easily-grasping mode. By means of the anti-contamination outer sleeve, the cleaning and the disinfecting of the cow vulva are not needed, contamination caused in the artificial insemination process is avoided, occurrence of reproductive system diseases is reduced, the inseminated rate of cattle is improved, and the conception rate of the artificial insemination of the cows is improved.
Description
Technical field
The present invention relates to the livestock reproduction technology, artificial insemination operational approach and the device of especially a kind of cattle.
Background technology
At present, the application of technology of artificial insemination has been brought into play important function to China's cattle breeds improvement and cultivation, is an indispensable key technology during cattle is cultured.Under good feeding and management condition, the cattle non-return rate can reach more than 50%, and a year over-all conception rate can reach more than 85%～90% even 95%.But the artificial insemination misoperation can cause the cows conception rate low and cause the breeding disease.The cattle technology of artificial insemination in; Polluted for fear of spermatic gun; Need draw clean cow rectum place excrement with hands, and first pudendum to cow carries out cleaning and sterilizing before semen deposition, still; Such method is when cleaning pudendum and semen deposition operation, and cow also can defecate pollute pudendum and spermatic gun head; And two kinds of extreme cases of such method existence, a kind of is to cause excessive cleaning and sterilizing, makes disinfectant solution get into vagina, brings the uterus during semen deposition into, and kills sperm; Another kind of then be that the cleaning of pudendum is not enough, bring feces into vagina during operation, pollute vagina and sperm is caused damage.
Summary of the invention
The objective of the invention is: artificial insemination method and the device of a kind of cattle are provided, and it can improve the test-tube conception rate of cattle, and simplifies test-tube operation, reduces the possibility that pollutes in the artificial insemination process, to overcome the deficiency of prior art.
The present invention is achieved in that the test-tube operational approach of cattle; Before spermatic gun is inserted the vagina of cattle; Earlier anti-pollution overcoat is inserted cow vagina deep, again a hands is goed deep into rectum and carry out the artificial insemination operation, in the rectum hold cervix uteri in hand after; Another holds spermatic gun; The spermatic gun of the rifle head of spermatic gun through anti-pollution overcoat inserts a mouthful mesopore and spermatic gun is inserted the cow cervix uteri carries out semen deposition, thereby saved when artificial insemination the cleaning and sterilizing program of cow pudendum, can prevent that spermatic gun is contaminated when semen deposition is operated.
Before carrying out semen deposition operation, to observations of oestrusing of cow, change into 3 times from 2 times, concrete grammar is: observe on the basis of oestrusing in every morning and afternoon, in late 20～22 increases observation of once oestrusing, observing time is greater than 1h at every turn; Adopt visual observation to combine examination per vagina evaluations of oestrusing, observing stands oestrus back 6h～10h or be judged as according to the variation of pudendum and secretions and carry out semen deposition when oestrusing the later stage.Cow has characteristics such as estrus duration is short, ovulation is fast, the end back ovulation of oestrusing; Accurately grasping estrus time is the key factor that improves the cow conception rate; And oestrusing of cow identifies that peasant household and scale vaccary for cowboying are difficult problems; Be easy to identify the untimely service period of missing, prolong the calving interval, reduce reproductive efficiency because of oestrusing.The present invention is through increasing number of observation and the time to estrus of cow, and is chosen in suitable time and observes, and effectively improved the comprehensive and accuracy that estrus of cow is identified, the leakage that reduces cows is joined and mismatched phenomenon, has improved awarding of cows and has joined rate.
The clear and definite thawing time of straw frozen semen; Defreezing method is: it is that 40 ± 2 ℃ hot water shakes 15～20s that straw frozen semen is put into temperature; Take out the water mark of drying tubule surface with sterile gauze then, after the sealing end of tubule being cut off, the good tubule that will the thaw spermatic gun of packing into is for use again.
Adopt straw frozen semen to carry out artificial transmission & distribution and want much less than effective sperm count that natural mating gets into gential tract of dam; Therefore require to use when freezing smart transmission & distribution; Want strict control to thaw and freeze smart temperature and time; The quality of seminal fluid all was input to intrauterine to the seminal fluid of every part after assurance was thawed, and could guarantee that female animal has higher conception rate.To finding in the investigation that semen deposition person is in the operation phenomenon lack of standardization, unscientific when freezing essence of thawing; The present invention has specified a cover to implement easily; The program that easy to operate straw frozen semen thaws has effectively been avoided having improved the test-tube conception rate of cattle because of improper 2 injuries that seminal fluid is caused of thawing.
Used anti-pollution overcoat is used the bromo geramine cleaning and disinfecting earlier, and reuse flushing with clean water 4～5 times is put into calorstat then, is antibacterial 120min under 80 ℃ the condition in temperature; After anti-pollution overcoat after maybe will cleaning is dried moisture, use power to be 30W ultraviolet radiation disinfection 20min, use plastic film packaging for use at last; After anti-pollution overcoat after maybe will cleaning is dried moisture, wrap, carry out retort sterilization again with antiseptic gauze.
Be used for the test-tube anti-pollution overcoat of cattle, comprise the polypropylene body of hollow, be provided with anti-scratch boss at the front end of polypropylene body, on preceding half section of polypropylene body, be provided with anti-skidding boss, the outer surface of anti-scratch boss and anti-skidding boss is circular arc; End at the polypropylene body is provided with spermatic gun insertion mouth.Anti-scratch boss is smooth hemispherical, when inserting the cow vagina, can prevent the scratch vagina; Anti-skidding boss is a hemispherical, can prevent that anti-pollution overcoat is from the vagina slippage.
It is funnel-form that spermatic gun inserts mouth, and the diameter of major part is 2～2.5 cm, and the diameter of osculum is 0.5cm, and the angle of inclination of sidewall is 25～35 °.
The length of polypropylene body is 27～29cm, and the external diameter of polypropylene body is that 0.7cm, its internal diameter are 0.5cm; The spacing of anti-scratch boss and anti-skidding boss is 8～10cm; The external diameter of anti-scratch boss and anti-skidding boss is 0.85cm.
Before the tradition semen deposition, need draw clean cow rectum place excrement, cleaning and sterilizing cow pudendum and wiped clean then with hands.This operating process is not only loaded down with trivial details, and is prone to reduce test-tube conception rate, and reason is: (1) when cleaning pudendum and semen deposition operation, cow also can defecate pollute pudendum and spermatic gun head; (2) in cleaning process, be prone to bring water, disinfectant, dirt into vagina, can make dirt, disinfectant get into the uterus during semen deposition, dirt contains a large amount of antibacterials, can infect the uterus, and disinfectant can kill sperm, directly influences smart ovum and combines, and reduce conception rate.
The present invention adopts anti-pollution overcoat, saves the step of the cleaning cow pudendum in the cattle artificial insemination operation, reduces vaginal orifice fecal pollution thing to murdering of sperm and importing into of vagina; Because what anti-pollution overcoat adopted is the polypropylene body, its poor thermal conductivity can reduce the stimulation to the milch cow vagina; Thereby alleviate the Cervical anxiety of cow; Help spermatic gun and get into the cervix uteri deep, alleviate, reach the purpose that improves conception rate the injury of vaginal mucosa and the generation of genital diseases.
The present invention 2010 to 2011; Apply second cattle farm of three dairy industry companies, milk cattle cultivating sub-district, Qingzhen and Wudang District milk cattle cultivating sub-district in the Guiyang; Adopt technical scheme of the present invention that the milch cow of above-mentioned cattle farm is carried out artificial insemination; And the situation after will inseminating carries out record, the sickness rate of the non-return rate in 2010,2011 of statistical test cows, year over-all conception rate, breeding difficulty disease respectively, and compare with the statistical indicator in 2007,2008,2009.
Table 1 result demonstration, all lower at non-return rate, year conception rate of 2007,2008 and 2009 cows of suckling, and downward trend is year by year arranged.After adopting technical scheme of the present invention, cows all increased than preceding 3 years at non-return rate, year conception rate in 2010,2011, and were the trend that increases year by year.Non-return rate after the employing present technique has improved 6.3 percentage points, 5.5 percentage points than the average before adopting respectively with the average of year conception rate.Wherein, the non-return rate in 2010,2011 improved 6.4 percentage points, 9.3 percentage points respectively than 2009, and the year conception rate in 2010,2011 has improved 7.0 percentage points, 9.6 percentage points respectively than 2009.And the sickness rate of cows endometritis reduces year by year, explain adopt anti-pollution overcoat can reduce because of misoperation to damage and infection that the cattle reproductive system causes, both guaranteed effective combination of smart ovum, reduced the generation of inflammation of uterus again.
Owing to adopt above-mentioned technical scheme; Compared with prior art; The present invention to observe in oestrus of cow, straw frozen semen thaw and the semen deposition mode has been carried out more science, also more wield improvement; Effectively improved the comprehensive and accuracy that estrus of cow is identified, the leakage of minimizing cows is joined and is mismatched phenomenon, has improved awarding of cows and has joined rate; Stipulated to freeze smart thaw point and time, avoided because of improper 2 injuries that seminal fluid is caused of thawing; Adopt anti-pollution overcoat to substitute cleaning and sterilizing, avoided the pollution in the artificial insemination process, reduced the generation of reproductive system disease, improved the test-tube conception rate of cattle the cow pudendum.The present invention is simple, and the apparatus structure that is adopted is simple, makes easily, and result of use is desirable.
Description of drawings
Accompanying drawing 1 is a structural representation of the present invention.
The specific embodiment
Embodiments of the invention 1: the artificial insemination method of cattle: the confirming of (1) semen deposition time: observing the concrete grammar that cow gets into oestrus is; On the basis of prior art every morning and observation in the morning in the afternoon; Every night 20～22 observation of once oestrusing again, be more than the 1h each observing time; Adopt visual observation to combine examination per vagina evaluations of oestrusing, observe cow and stand and oestrus back 6～10h or judge that cow entering post-estrus carries out semen deposition; (2) thaw routine of straw frozen semen: it is that 40 ± 2 ℃ hot water shakes 15s that straw frozen semen is put into temperature; Take out the water mark of drying the tubule surface with sterile gauze then; Sealing end with tubule cuts off again; The good tubule that will the thaw spermatic gun of packing into is for use, and the good tubule that thaws is packed into and inseminated and will in 30min, accomplish to accomplishing behind the spermatic gun; (3) cleaning and sterilizing of anti-pollution overcoat: used anti-pollution overcoat is used the bromo geramine cleaning and disinfecting earlier, and reuse flushing with clean water 4～5 times is put into calorstat then, is antibacterial 120min under 80 ℃ the condition in temperature, uses plastic film packaging for use at last; (4) test-tube operational approach: anti-pollution overcoat is inserted cow vagina deep; Again a hands is goed deep into rectum and carry out the artificial insemination operation; In the rectum hold cervix uteri in hand after; Another holds spermatic gun, the rifle head of the spermatic gun spermatic gun through anti-pollution overcoat inserts a mouthful mesopore and spermatic gun is inserted the cow cervix uteri carries out semen deposition.
The structure that is used for the test-tube anti-pollution overcoat of milch cow is as shown in Figure 1; The polypropylene body 1 that comprises hollow; Front end at polypropylene body 1 is provided with anti-scratch boss 2; On preceding half section of polypropylene body 1, be provided with anti-skidding boss 3, the outer surface of anti-scratch boss 2 and anti-skidding boss 3 is circular arc; End at polypropylene body 1 is provided with spermatic gun insertion mouth 4, and it is funnel-form that spermatic gun inserts mouth, and the diameter of major part is 2.5cm, and the diameter of osculum is 0.5cm, and the horizontal tilt angle that spermatic gun inserts mouth 4 sidewalls is 30 °; The length of polypropylene body 1 is 29cm, and the external diameter of polypropylene body 1 is that 0.7cm, its internal diameter are 0.5cm; Anti-scratch boss 2 is 8cm with the spacing of anti-skidding boss 3; The external diameter of anti-scratch boss 2 and anti-skidding boss 3 is 0.85cm.
Embodiments of the invention 2: the artificial insemination method of cattle: the confirming of (1) semen deposition time: observe cow and get into post-estrus; Observing the concrete grammar that cow gets into oestrus is: observe on the basis of oestrusing in prior art every morning and in the morning in the afternoon; Every night 20～22 observation of once oestrusing again, be more than the 1h each observing time; Adopt visual observation to combine the examination per vagina evaluation of oestrusing, be judged as according to the variation of pudendum and secretions and carry out semen deposition when oestrusing the later stage; (2) thaw routine of straw frozen semen: it is 40 ± 2 ℃ water-bath jog 15s that straw frozen semen is put into temperature; Take out the water mark of drying the tubule surface with sterile gauze then; After again the sealing end of tubule being cut off; The good tubule that will the thaw spermatic gun of packing into is for use, and the good tubule that thaws is packed into and inseminated and will in 30min, accomplish to accomplishing behind the spermatic gun; (3) cleaning and sterilizing of anti-pollution overcoat: used anti-pollution overcoat use the bromo geramine cleaning and disinfecting earlier, reuse flushing with clean water 4～5 times, the anti-pollution overcoat after the cleaning dried moisture after, wrap with antiseptic gauze, carry out retort sterilization again; (4) test-tube operational approach: anti-pollution overcoat is inserted cow vagina deep; Again a hands is goed deep into rectum and carry out the artificial insemination operation; In the rectum hold cervix uteri in hand after; Another holds spermatic gun, the rifle head of the spermatic gun spermatic gun through anti-pollution overcoat inserts a mouthful mesopore and spermatic gun is inserted the cow cervix uteri carries out semen deposition.
Claims (7)
1. the test-tube operational approach of a cattle; It is characterized in that: before spermatic gun is inserted the vagina of cattle; Earlier anti-pollution overcoat is inserted cow vagina deep, again a hands is goed deep into rectum and carry out the artificial insemination operation, in the rectum hold cervix uteri in hand after; Another holds spermatic gun; The spermatic gun of the rifle head of spermatic gun through anti-pollution overcoat inserts a mouthful mesopore and spermatic gun is inserted the cow cervix uteri carries out semen deposition, thereby saved when artificial insemination the cleaning and sterilizing program of cow pudendum, can prevent that spermatic gun is contaminated when semen deposition is operated.
2. the test-tube operational approach of cattle according to claim 1; It is characterized in that: to the observation of oestrusing of cow; Change into 3 times from 2 times, concrete grammar is, on the basis of observing in every morning and afternoon; Increased evening 20～22 to oestrus observation of cattle, each observing time is greater than 1h; Adopt visual observation to combine examination per vagina evaluations of oestrusing, observing stands oestrus back 6h～10h or be judged as according to the variation of pudendum and secretions and carry out semen deposition when oestrusing the later stage.
3. the test-tube operational approach of cattle according to claim 1; It is characterized in that: the clear and definite thawing time of straw frozen semen; That is: before carrying out semen deposition operation, it is that 40 ± 2 ℃ hot water shakes 15～20s that straw frozen semen is put into temperature, takes out the water mark of drying the tubule surface with sterile gauze then; After the sealing end of tubule being cut off, the good tubule that will the thaw spermatic gun of packing into is for use again.
4. the artificial insemination method of cattle according to claim 1; It is characterized in that: used anti-pollution overcoat is used the bromo geramine cleaning and disinfecting earlier, and reuse flushing with clean water 4～5 times is put into calorstat then; In temperature antibacterial 120min under 80 ℃ the condition; After anti-pollution overcoat after maybe will cleaning is dried moisture, use power to be 30W ultraviolet radiation disinfection 20min, use plastic film packaging for use at last; After anti-pollution overcoat after maybe will cleaning is dried moisture, wrap, carry out retort sterilization again with antiseptic gauze.
5. one kind is used for the test-tube anti-pollution overcoat of cattle; The polypropylene body (1) that comprises hollow; It is characterized in that: the front end at polypropylene body (1) is provided with anti-scratch boss (2); On preceding half section of polypropylene body (1), be provided with anti-skidding boss (3), the outer surface of anti-scratch boss (2) and anti-skidding boss (3) is circular arc; End at polypropylene body (1) is provided with spermatic gun insertion mouthful (4).
6. the test-tube anti-pollution overcoat of cattle that is used for according to claim 5 is characterized in that: it is funnel-form that spermatic gun inserts mouthful (4), and the diameter of major part is 2～2.5 cm, and the diameter of osculum is 0.5cm, and the angle of inclination of sidewall is 25～35 °.
7. the test-tube anti-pollution overcoat of cattle that is used for according to claim 5, it is characterized in that: the length of polypropylene body (1) is 27～29cm, the external diameter of polypropylene body (1) is that 0.7cm, its internal diameter are 0.5cm; Anti-scratch boss (2) is 8～10cm with the spacing of anti-skidding boss (3); The external diameter of anti-scratch boss (2) and anti-skidding boss (3) is 0.85cm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012102803509A CN102743240A (en) | 2012-08-08 | 2012-08-08 | Method and device for performing artificial insemination on cows |
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| CN2012102803509A CN102743240A (en) | 2012-08-08 | 2012-08-08 | Method and device for performing artificial insemination on cows |
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| CN2012102803509A Pending CN102743240A (en) | 2012-08-08 | 2012-08-08 | Method and device for performing artificial insemination on cows |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107157612A (en) * | 2017-03-29 | 2017-09-15 | 吴学权 | It is a kind of to improve the method that frozen cattle semens transmission ＆ distribution award tire rate |
| CN110866481A (en) * | 2019-11-07 | 2020-03-06 | 北京小龙潜行科技有限公司 | Sow oestrus detection method and device |
| CN113749045A (en) * | 2021-09-14 | 2021-12-07 | 毕节市畜牧兽医科学研究所 | Method for improving cow breeding efficiency |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107157612A (en) * | 2017-03-29 | 2017-09-15 | 吴学权 | It is a kind of to improve the method that frozen cattle semens transmission ＆ distribution award tire rate |
| CN110866481A (en) * | 2019-11-07 | 2020-03-06 | 北京小龙潜行科技有限公司 | Sow oestrus detection method and device |
| CN110866481B (en) * | 2019-11-07 | 2023-06-30 | 北京小龙潜行科技有限公司 | Sow oestrus detection method and device |
| CN113749045A (en) * | 2021-09-14 | 2021-12-07 | 毕节市畜牧兽医科学研究所 | Method for improving cow breeding efficiency |
| CN113749045B (en) * | 2021-09-14 | 2023-03-14 | 毕节市畜牧兽医科学研究所 | Method for improving cow breeding efficiency |
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