CN102715193B - Bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide and use thereof - Google Patents

Abstract

The invention relates to a compound fungicide, wherein the effective active ingredients are composed of Bacillus subtilis sf-628 and prochloraz manganese salt. It is characterized in that: among the active ingredients, the weight ratio of bacillus subtilis sf-628 to prochloraz manganese salt is: 1-99:99-1. The compound bactericide is a wettable powder, and its composition and content ratio are respectively: 50% prochloraz manganese salt: 30%; 20 billion Bacillus subtilis sf-628 wettable powder: 30%; NNO (methylene di Sodium naphthalenesulfonate) is 7%; polyethylene glycol is 3%; sodium dodecylbenzenesulfonate is 5%; light calcium carbonate is 25%. During application, the above-mentioned compound fungicide is diluted and poured into the roots of vegetables to prevent and control soil-borne diseases of vegetables, mainly fusarium wilt and bacterial wilt of solanaceous crops; the content of the diluted effective drug components is at least 300mg/L. Its advantages are: the mixed use of Bacillus subtilis sf-628 and the chemical agent prochloraz manganese salt in a certain proportion can realize complementary advantages, reduce the dosage of chemical agents, reduce the cost of medication, and improve the ecological environment while improving the control effect , Reduced the residues of chemicals in agricultural products.

Description

Bacillus subtilis sf-628 and prochloraz manganese salt compound fungicide and its application

technical field

The present invention relates to a compound fungicide and its application, especially the fungicide compounded by a strain of Bacillus subtilis sf-628 and prochloraz manganese salt and its application in preventing and treating soil-borne diseases of solanaceous crops (fusarium wilt and bacterial wilt) application.

Background technique

With the improvement of people's living standards, the requirements for food safety are getting higher and higher. Ensuring the supply of pollution-free and high-quality agricultural products and realizing the sustainable use of resources are the biggest challenges facing agricultural production in the 21st century. On the one hand, a substantial increase in the planting area of protected vegetables is the core content of the structural adjustment of the planting industry; on the other hand, the high-yield, continuous cropping, and diversified planting layout of protected vegetables provides sufficient nutrition and breeding conditions for pests and diseases. Therefore, frequent outbreaks of soil-borne diseases (fusarium wilt and bacterial wilt, etc.) of solanaceous crops under facility conditions in recent years have resulted in the severe situation of continuous use of chemical pesticides during the vegetable growth period. The method of using chemical agents to prevent and control soil-borne diseases of vegetables not only causes serious pollution to the ecological environment, but also directly increases the residues of toxic chemicals in vegetables, which brings serious harm to human health. How to reduce the chemical residues in vegetables has become the focus of governments at all levels, scholars and vegetable farmers. Biopesticides are safe for humans and animals and have good environmental compatibility. Therefore, the use of biological pesticides to control soil-borne diseases of vegetables has attracted more and more attention. However, there are still some problems when using biopesticides in the field, such as: unstable control effect in the field, slow drug effect and so on.

The fundamental way to solve this outstanding problem is to break through the limitations of the current control measures based on chemical pesticides, and develop a technical system for synergistic control of soil-borne diseases of solanaceous crops by biocontrol microorganisms and low-toxic chemicals. By purposely introducing beneficial microorganisms, deteriorating the living environment of pathogenic bacteria and forming complementary advantages, it can not only exert the efficient and rapid antibacterial effect of chemical agents, but also exert the role of biocontrol microorganisms in inducing plant disease resistance and lastingly controlling solanaceous vegetables The purpose of soil-borne disease epidemics.

Bacillus subtilis sf-628 screened by Jiangsu Academy of Agricultural Sciences, which has good control effect on various fungal diseases, was approved by the General Microorganism Center of China Committee for Culture Collection of Microorganisms (CGMCC) on August 1, 2006. ) accepted for deposit, the deposit number is: CGMCCNo.1772, and the Latin scientific name is Bacillus subtilis sf-628 (hereinafter referred to as sf-628). Its biological properties are recorded as: sf-628 colonies are round, beige, and wrinkled on the surface. Positive Gram reaction, cell shape: rod-shaped, cell diameter greater than 1 μm, strictly aerobic, L-arabinose, D-glucose, D-xylose, D-mannitol, starch hydrolysis, gelatin liquefaction and other utilization reactions are all positive .

Since 2007, Jiangsu Provincial Academy of Agricultural Sciences has carried out the research of Bacillus subtilis sf-628 on the prevention and treatment of pear ring spot, and related reports (see Acta Pomology 2010, 27 (5) for details; 2010, 27 (1); 2009, 26 (3); Jiangsu Agricultural Journal 2009, 25(5)). In recent years, the laboratory has found that Bacillus subtilis sf-628 also has a strong control effect on vegetable wilt and bacterial wilt.

Bacillus subtilis sf-628 is a strain that has natural resistance to the chemical agent prochloraz manganese salt, and further resistance induction with prochloraz manganese salt, Bacillus subtilis sf-628 can produce 10000ppm prochloraz manganese salt Grow normally on YGPA medium. Bacillus subtilis sf-628 also has many excellent properties, such as: a broad antibacterial spectrum; fast growth, strong ability to adapt to the environment, can colonize soil and plant surfaces, and form dominant populations; induce hosts to develop disease resistance Sex; secrete and produce a variety of antibacterial substances.

At present, Jiangsu Suke Agrochemical Co., Ltd. has produced 20 billion wettable powders of Bacillus subtilis sf-628.

Prochloraz manganese salt, English common name prochloraz-manganese chloride complex; chemical name is N-propyl-N[2-(2,4,6-trichlorophenoxy)ethyl]-1H imidazole-1-methyl Amide - Manganese Chloride. Other names: Shi Baogong. The appearance of the original drug is white to brown sand-like powder, with a slightly aromatic smell, a melting point of 141-142.5°C, a solubility in water of 40mg/L, and is easily soluble in organic solvents. The vapor pressure is 0.02Pa (20°C). In aqueous solution or suspension, the complex is separated quickly, and its separation degree reaches 55°C within 4 hours at 25°C, which belongs to low-toxicity fungicides. Acute oral LD50 of the original drug in rats: 1600-3200mg/kg. Prochloraz manganese salt has dual effects of treatment and protection, strong systemic absorption, can be absorbed by roots, stems and leaves, and can quickly conduct upwards in the plant body, and can effectively prevent and control diseases caused by most fungi. Its mechanism of action is to affect the biosynthesis of sterols, destroy the cell membrane function of pathogenic bacteria, and eventually lead to cell death, thereby playing the role of sterilization, disease prevention and treatment. Prochloraz manganese salt has good control effect on soil-borne diseases of solanaceous crops.

At present, the wettable fungicidal powder compounded by Bacillus subtilis sf-628 and prochloraz manganese salt has not been reported.

Contents of the invention

The object of the present invention is to provide a kind of Bacillus subtilis (Bacillus subtilis) sf-628, and this bacterial strain has been accepted and preserved by the General Microorganism Center of China Microorganism Culture Collection Management Committee on August 1, 2006, and the preservation number is: CGMCC No. 1772.

Another purpose of the present invention is: in view of the problem that a large amount of chemical pesticides are used in the current production to prevent and control vegetable diseases, seriously pollute the ecological environment, and cause pesticide residues in agricultural products to exceed the standard, propose a plant that is effective against soil-borne diseases of solanaceous crops (fusarium wilt, cyanosis, etc.) Bacillus subtilis sf-628 and prochloraz manganese salt compound wettable fungicide powder with good control effect and its application. After compounding Bacillus subtilis sf-628 and prochloraz manganese salt, they form a complementary effect, which can not only exert the efficient and rapid antibacterial effect of chemical agents, but also exert the effect of Bacillus subtilis sf-628 on inducing plant disease resistance , the purpose of permanently controlling the prevalence of soil-borne diseases of solanaceous vegetables

The inventor screens out Bacillus subtilis sf-628 from 2000 bacterial strains obtained by sampling and isolation, and the antibacterial test results show that the bacterial strain has a strong inhibitory effect on various fungal diseases; and Bacillus subtilis sf-628 is a strain The strains that are naturally resistant to the chemical prochloraz manganese salt, through resistance induction, Bacillus subtilis sf-628 can grow normally on the YGPA medium with the prochloraz manganese salt content of 10000ppm. Therefore, Bacillus subtilis sf-628 and prochloraz manganese salt can be compounded into a fungicide to achieve long-term coexistence.

The invention provides a wettable powder for preventing and treating fusarium wilt and bacterial wilt of solanaceous crops, which is characterized in that its composition and weight percentage are as follows: active ingredient 10-85%; auxiliary agent 1-20% Binder 2-20%; Surfactant 1-20%; Filler 10-70%; Wherein active ingredient is made up of Bacillus subtilis (Bacillus subtilis) sf-628 and prochloraz manganese salt, the weight of both The ratio is 1-3:3-1, and the preservation number of the above-mentioned strain is: CGMCC No.1772; wherein the auxiliary agent is selected from one or more of NNO, azone, and thiazone; the binder is selected from polyvinylpyrrolidone One or more of , polyethylene glycol, polyvinyl alcohol; surfactant is selected from one or more of sodium dodecylbenzene sulfonate, sodium lignin sulfonate, tea dry powder, saponin powder The filler is selected from one or more of light calcium carbonate, diatomaceous earth, kaolin, sericite powder, and white carbon black.

The wettable powder of the present invention has the following composition and weight percentage content: 50% prochloraz manganese salt: 30%; 20 billion Bacillus subtilis sf-628 wettable powder: 30%; NNO: 7%; polyethylene Glycol: 3%; Sodium Dodecyl Benzene Sulfonate: 5%; Light Calcium Carbonate 25%.

The advantages of the present invention are: by introducing Bacillus subtilis sf-628, which is safe for humans and animals and has a strong inhibitory effect on Fusarium wilt and R. Compounding in a certain proportion can achieve complementary advantages, reduce the amount of chemical agents used, and reduce the cost of medication. While improving the control effect, the ecological environment is improved and the residual amount of chemical agents in vegetables is reduced.

Detailed ways:

Embodiment 1,

Inhibition of Bacillus subtilis sf-628 against Fusarium wilt and Ralstonia solanacearum.

Pathogen strains tested:

Fusarium wilt of tomato was provided by Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. Use PSA medium (200g potato, 20g sucrose, 20g agar, and 1000mL distilled water, pH 5.6-6.6) to culture at 28°C for 5 days, and set aside.

Tomato solanacearum was provided by the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. Use NA medium (3.0 g of beef extract, 1 g of yeast extract, 5 g of peptone, 10 g of glucose, 20 g of agar, and 1000 mL of distilled water) at 28°C for 2 days with shaking (150 r/m), and set aside.

The Bacillus subtilis sf-628 screened by Jiangsu Academy of Agricultural Sciences was activated on NA medium, then transferred into 50 mL NA culture medium, cultured at 28°C with shaking (150r/m) for 2 days, and set aside. Place the Fusarium wilt bacteria block in the center of the PSA plate, with a diameter of 7mm; place 4 filter paper sheets in a cross shape around the petri dish (the distance from the center of the petri dish is 22.5mm), and add different dilutions of Bacillus subtilis sf- 7 μL of 628 bacterial solution; set water as control. Repeat 3 dishes for each treatment. Incubate at a constant temperature of 28°C. When the pathogenic bacteria colonies on the control plate are overgrown, measure the growth diameter of the treated colonies. Calculation of inhibition rate: inhibition rate (%)=[(control colony diameter-treatment colony diameter)/(control colony diameter-7)]×100%

Aspirate R. solanacearum liquid and coat NA plate (0.3mL bacterial liquid/plate). A piece of sterilized filter paper was placed in the center of the petri dish, and 7 μL of Bacillus subtilis sf-628 bacterial solution of different dilution multiples were added to the filter paper; water was used as a control. Repeat 3 dishes for each treatment. Incubate at a constant temperature of 28°C for 2 days, and measure the diameter of the inhibition zone.

The test results are shown in Table 1. The stock solution, 100-fold dilution and 500-fold dilution of Bacillus subtilis sf-628 all had strong inhibitory effects on Fusarium wilt and R. solanacearum.

Table 1 Inhibitory effect of Bacillus subtilis sf-628 on tomato Fusarium wilt and Ralstonia solanacearum

Example 2

Toxicity determination of different ratios of Bacillus subtilis sf-628 and prochloraz manganese salts against Fusarium wilt and Ralstonia solanacearum

(1) For the test bacteria:

Tomato Fusarium wilt and Ralstonia solanacearum were used as the test bacteria (provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences).

(2) Test drug:

A. 50% prochloraz manganese salt EC was produced and provided by Jiangsu Huifeng Agrochemical Co., Ltd.

B. 20 billion Bacillus subtilis sf-628 wettable powder is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

Indoor synergistic bioassay:

Mix 50% prochloraz manganese salt EC into 0.01μg/mL, 0.1μg/mL, 1μg/mL, 10μg/mL PSA medium plate with medicine; mix 20 billion Bacillus subtilis sf-628 wettable powder into 2×10 ⁶ cfu Bacillus subtilis/mL, 2×10 ⁵ cfu Bacillus subtilis/mL, 2×10 ⁴ cfu Bacillus subtilis/mL, 2×10 ³ cfu Bacillus subtilis/mL PSA medium plate with bacteria; According to the weight ratio of effective drug components of A and B, 1: 3; 1: 2; 1: 1; 2: 1; 10,000 times, 20,000 times, 40,000 times, prepared into PSA medium plates with different gradient drug solutions. Put the Fusarium wilt bacteria (7mm in diameter) into the center of the poisonous plate, and use a single dose of prochloraz manganese salt and a single dose of Bacillus subtilis sf-628 as controls. At the same time, set up a clean water control. When the clean water control plate is full, measure the colony diameter.

Calculation of inhibition rate: inhibition rate (%)=[(control colony diameter-drug treatment colony diameter)/(control colony diameter-7)]×100%

50% prochloraz manganese salt EC, 20 billion wettable powders of Bacillus subtilis sf-628, and 5 compound solutions of A and B were respectively prepared into drug solutions with different gradients (same as above, Example 2). Aspirate R. solanacearum liquid and coat NA plate (0.3mL bacterial liquid/plate). A piece of sterilized filter paper was placed in the center of the culture dish, and 7 μL each of prochloraz manganese salt solution, Bacillus subtilis sf-628 bacterial solution and compound solution of different dilution multiples were added to the filter paper; clean water was used as a control. Repeat 3 dishes for each treatment. Incubate at a constant temperature of 28°C for 2 days, and measure the diameter of the inhibition zone.

Inhibition rate (%) = (inhibition zone diameter / control colony diameter) × 100%

The observation value of the inhibition rate is transformed into the equivalent deviation of the inhibition rate, and the logarithm of the fungicide concentration in the culture medium of each treatment is X, and the equivalent deviation of the corresponding treatment on the growth inhibition rate of the pathogenic mycelium is Y, and statistical regression is applied Methods, the concentration logarithm-inhibition percentage probability value toxicity curve was fitted, and the EC ₅₀ value and SR were calculated. Refer to Wadley's formula (synergism index SR=theoretical EC ₅₀ of the mixture/measured EC ₅₀ of the mixture, SR>1.5 means synergistic effect, 0.5≤SR≤1.5 means additive effect, SR<0.5 means antagonistic effect).

The experimental results are shown in Table 2 and Table 3. The results of the virulence determination of Fusarium wilt of tomato showed that the synergistic indices of A:B=1:1, 2:1 and 3:1 were all greater than 1.5, indicating that the compound had a synergistic effect under these three ratios; The results of the virulence assay of R. solanacearum showed that the synergistic indices of A:B=1:3, 1:2, 1:1 and 2:1 were all greater than 1.5, indicating that the compound had a synergistic effect under these four ratios. .

Table 2 Determination of the synergistic effect of different ratios of Bacillus subtilis sf-628 and prochloraz manganese salt compound fungicides on inhibiting the mycelial growth of Fusarium wilt

Table 3 Determination of the synergistic effect of different ratios of Bacillus subtilis sf-628 and prochloraz manganese salt compound fungicides on inhibiting the growth of R. solanacearum

Example 3

Preparation of 15% prochloraz manganese salt·6 billion bacillus subtilis sf-628 wettable powder

Effective drugs:

A. 50% prochloraz manganese salt EC was produced and provided by Jiangsu Huifeng Agrochemical Co., Ltd.

B. 20 billion Bacillus subtilis sf-628 wettable powder is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

The auxiliary agent is NNO (sodium methylene dinaphthalene sulfonate); the binder is polyethylene glycol; the surfactant is sodium dodecylbenzene sulfonate; the filler is light calcium carbonate.

Each component is prepared according to the following weight percentages:

50% prochloraz manganese salt: 30%; 20 billion Bacillus subtilis sf-628 wettable powder: 30%; NNO (sodium methylene dinaphthalene sulfonate): 7%; polyethylene glycol: 3%; ten Sodium Dialkylbenzene Sulfonate: 5%; Light Calcium Carbonate: 25%.

Preparation process: put prochloraz manganese salt, Bacillus subtilis sf-628, etc. in the mixer according to the above proportions, mix evenly, grind to 325 mesh with a jet mill, sieve, pack, and prepare 15% prochloraz Manganese salt · 6 billion Bacillus subtilis sf-628 wettable powder.

Embodiment 4,

Determination of the synergistic effect of 15% prochloraz manganese salt·6 billion Bacillus subtilis sf-628 wettable powder on the growth of tomato Fusarium wilt and Ralstonia solanacearum

Test agent: 15% prochloraz manganese salt·6 billion Bacillus subtilis sf-628 wettable powder provided by Example 3.

Contrast agent: 50% prochloraz manganese salt EC (produced by Jiangsu Huifeng Agrochemical Co., Ltd.); 20 billion Bacillus subtilis sf-628 wettable powder (produced by Jiangsu Suke Agrochemical Co., Ltd.).

Indoor synergistic bioassay:

Mix 50% prochloraz manganese salt EC into 0.01μg/mL, 0.1μg/mL, 1μg/mL, 10μg/mL PSA medium plate with medicine; mix 20 billion Bacillus subtilis sf-628 wettable powder into 2×10 ⁶ cfu Bacillus subtilis/mL, 2×10 ⁵ cfu Bacillus subtilis/mL, 2×10 ⁴ cfu Bacillus subtilis/mL, 2×10 ³ cfu Bacillus subtilis/mL PSA medium plate with bacteria; Dilute 15% prochloraz manganese salt·6 billion Bacillus subtilis sf-628 wettable powder by 2500 times, 5000 times, 10000 times, 20000 times and 40000 times to prepare PSA medium plates with different gradients of liquid medicine. Then put the tomato Fusarium wilt bacteria (7mm in diameter) into the center of the plate, and use a single agent of prochloraz manganese salt and a single agent of Bacillus subtilis sf-628 as a control, and set up a clear water control plate at the same time. When the clear water control plate is full, measure the colony diameter .

50% prochloraz manganese salt EC, 20 billion Bacillus subtilis sf-628 wettable powder, 15% prochloraz manganese salt·6 billion Bacillus subtilis sf-628 wettable powder were prepared into compound medicines with different gradients solution (same as above, Example 4). Draw the R. solanacearum liquid and coat the NA plate (0.3mL bacterial liquid/plate). A sterilized filter paper was placed in the center of the petri dish, and 7 μL each of prochloraz manganese salt solution, biocontrol bacteria solution and compound agent of different dilution multiples were added to the filter paper; clean water was used as a control. Repeat 3 dishes for each treatment. Incubate at a constant temperature of 28°C for 2 days, and measure the diameter of the inhibition zone.

Inhibition rate and synergy index SR calculation are the same as in Example 2.

The test results are shown in Table 4 and Table 5. The test results of the toxicity test of 15% prochloraz manganese salt·6 billion Bacillus subtilis sf-628 wettable powder on tomato Fusarium wilt and R. solanacearum showed that the synergistic index was 1.56 respectively and 2.57, both have synergistic effects.

Table 4 15% prochloraz manganese salt · 6 billion Bacillus subtilis sf-628 wettable powder inhibits the synergistic determination of tomato Fusarium wilt mycelia growth

Table 5 15% prochloraz manganese salt · 6 billion Bacillus subtilis sf-628 wettable powder inhibits synergistic determination of tomato Ralstonia solanacearum

Embodiment 5,

Field plot effect of 15% prochloraz manganese salt·6 billion Bacillus subtilis sf-628 wettable powder on control of tomato wilt and bacterial wilt

Test time: around November 1, 2009 (tomato seeding) in the tomato greenhouse of a contracted household in Dajian Village, Huachong Town, Shuyang County, Jiangsu Province.

Test variety: Hongbaoyu.

Test medicament and comparative medicament: same as embodiment 4.

Field drug efficacy test: when tomato seedlings are transplanted (at the end of December), the roots are irrigated, and the roots are irrigated every two weeks or so (15 days) after transplanting. A total of 8-9 times of medication are required throughout the growth period. Dilute the finished medicine by 500 to 1600 times, and the content of the diluted active pharmaceutical ingredients should be at least 300 mg/L; each treatment should be repeated twice, and a protection line should be set.

The incidence index of tomato fusarium wilt and bacterial wilt in each plot was investigated in the first ten days of March. The grading standards are as follows: grade 0 means that the vascular bundles in the stem are normal and the outside is asymptomatic; grade 1 means discoloration of less than 1/4 of the vascular bundles in the stem; Discoloration of 1/4-1/2 of the vascular bundles in the stem; grade 5, discoloration of 1/2-3/4 of the vascular bundles in the stem; grade 7, discoloration of more than 3/4 of the vascular bundles in the stem, and partial wilting of leaves; grade 9, whole The plant dies.

Disease index = [(number of incidences at all levels × representative value at each level)/(total number of plants under investigation × representative value at the highest level)] × 100

Prevention and treatment effect (%)=[(the disease index of the control-the disease index of the control)/the disease index of the control]×100%

Field test results (Table 6) show that the control effects of a single dose of 50% prochloraz manganese salt wettable powder (125 grams per mu, diluted 1600 times) to tomato wilt and bacterial wilt are 60.74% and 49.07%, respectively. The control effects of a single dose of 20 billion Bacillus subtilis sf-628 wettable powder (125 g per mu, diluted 1600 times) on tomato wilt and bacterial wilt were 59.42% and 62.09%, respectively.

15% prochloraz manganese salt·6 billion bacillus subtilis sf-628 wettable powder with an amount of 400 grams per mu (diluted 500 times) has the best control effect on tomato fusarium wilt and bacterial wilt, which are 83.41% and 80.21% respectively . The content of active ingredients in the compound fungicide with an dosage of 125 grams per mu (diluted 1600 times) only accounts for about 1/3 of the two single agents, but the control effect is slightly lower than that of the control two single agents.

The control effects of the compound fungicide with an amount of 200 grams per mu (diluted 1000 times) to tomato fusarium wilt and bacterial wilt were 72.85% and 70.22% respectively; /2, but the control effect is obviously better than the two single agents (see Table 6).

Table 6 15% prochloraz manganese salt · 6 billion Bacillus subtilis sf-628 wettable powder control effect of tomato wilt and bacterial wilt field plot

Embodiment 6,

Field plot effect of 15% prochloraz manganese salt·6 billion Bacillus subtilis sf-628 wettable powder on control of eggplant wilt and bacterial wilt

Test time: around October 1, 2010 (dropping seeds) in the facility greenhouse of Sucheng Modern Agriculture Industrial Park, Shuyang County, Jiangsu Province.

Test variety: Su Qi No. 1.

Test medicament and comparative medicament: same as embodiment 4.

Field drug efficacy test, disease grading standard and control effect calculation: same as embodiment 5.

Field test results (Table 7) show that the control effects of a single dose of 50% prochloraz manganese salt wettable powder (125 grams per mu, diluted 1600 times) on eggplant wilt and bacterial wilt are 54.61% and 41.73%, respectively. The control effects of a single dose of 20 billion Bacillus subtilis sf-628 wettable powder (125 g per mu, diluted 1600 times) on tomato wilt and bacterial wilt are 63.68% and 58.91% respectively.

The changing trend of the usage per mu of the compound agent is the same as that of Example 5. The control effect of the 500-fold dilution is the best; the 1000-fold dilution is next; the control effect of the 1600-fold dilution is slightly worse than the two single agents.

Wherein the content of active ingredient in the 1000-fold dilution accounts for about 1/2 of the two single agents, but the control effect is obviously better than the two single agents (see Table 7).

Table 7 15% prochloraz manganese salt · 6 billion bacillus subtilis sf-628 wettable powder control effect of eggplant wilt and bacterial wilt field plot

Claims (2)

1. A wettable powder for preventing and treating fusarium wilt and bacterial wilt of solanaceous crops, characterized in that: its composition and weight percentage are as follows: active ingredient 10-85%; auxiliary agent 1-20%; 2-20% mixture; 1-20% surfactant; 10-70% filler; wherein the active ingredient is composed of Bacillus subtilis sf-628 and prochloraz manganese salt, and the weight ratio of the two is 1 -3:3-1, the preservation number of the above-mentioned strain is: CGMCC No.1772; wherein the auxiliary agent is selected from one or more of NNO, azone, and thione; the binder is selected from polyvinylpyrrolidone, polyethylene glycol One or more of glycol, polyvinyl alcohol; surfactant is selected from one or more of sodium dodecylbenzene sulfonate, sodium lignin sulfonate, tea dry powder, saponin powder; filling The agent is selected from one or more of light calcium carbonate, diatomaceous earth, kaolin, sericite powder, and white carbon black. 2. The wettable powder according to claim 1, its composition and weight percentage are as follows: 50% prochloraz manganese salt: 30%; 20 billion Bacillus subtilis sf-628 wettable powder: 30%; NNO: 7%; Macrogol: 3%; Sodium Dodecylbenzene Sulfonate: 5%; Light Calcium Carbonate 25%.