CN102715004B - A kind of method that improves ornamental plant resistance - Google Patents

Abstract

The invention discloses a method for improving stress resistance of garden plants, which belongs to the technical field of garden plant protection. It includes the following steps: 1) Prepare the mycorrhizal fungus spore suspension; 2) Germinate the plant seeds at 23-28°C, soak in the mycorrhizal fungal suspension for 5-12 minutes when the seeds are 1/2 white, and then sow into peat: The nutrient soil volume ratio is 3-5:1 in the cultivation soil, and after the seeds germinate for 60-80 days, inject 2-6ml/strain of mycorrhizal spore suspension with a syringe at a distance of 2-3cm from the root of the seedling, and then use Cultivate the soil to cover and compact the injection site, and transplant after 6-8 months of cultivation, and then spray the mycorrhizal spore suspension after transplanting. The above-mentioned method for improving the stress resistance of garden plants has multiple effects such as promoting seed vigor, improving the synthesis of seedling mycorrhiza, promoting seedling growth, and improving the stress resistance of plants, and can effectively resist the occurrence of various diseases. The saplings have special effects on stress resistance.

Description

A method for improving stress resistance of garden plants

technical field

The invention belongs to the technical field of garden plant protection, and specifically relates to a method for improving stress resistance of garden plants.

Background technique

Mycorrhizal fungi (mycorrhizalfungi) are a type of symbiotic fungi that can form a symbiosis with the young part of the plant rhizosphere in the soil, which can improve the stress resistance of the host plant (including drought resistance, salt resistance and disease resistance, etc.), Promote the growth of seedlings and enhance the host's ability to resist pollution from heavy metals such as Cd ²⁺ . According to Song Fuqiang and other studies, the average survival rate of mycorrhizal afforestation is over 95%, which can achieve the effect of a successful afforestation. It is an indispensable biotechnology in forestry production. Tang Ming et al. investigated the resources of mycorrhizal fungi in many semi-arid areas in Northwest my country, studied the effects of mycorrhizal fungi on the drought resistance of seabuckthorn and other plants, and discussed how mycorrhizal fungi can improve poplar's resistance to canker. To a certain extent, it promotes the development and utilization of mycorrhizal resources in my country. As a typical ectomycorrhizal fungus, Paxillus involutus (BatschexFr.) Fr. is widely distributed in the northern hemisphere, and it is easier to form mycorrhizal fungi after inoculation. Because P.involutus can effectively improve the stress resistance of host plants and promote the growth of host plants, many researches have been carried out on the development and utilization of P.involutus at home and abroad, and great progress has been made. Quoreshi and Khasa found that when P.involutus was inoculated together with the bacteria Burkholderiacepacia , it could significantly improve the absorption of nutrients such as N and P in poplar P.tremuloides and balsam poplar P.angustifolia , thereby improving root growth. According to Garbaye et al., the mycorrhizae formed by P.involutus can significantly improve the drought resistance of Quercuspetraea and Q.robur trees. In addition, P. involutus also has a strong absorption effect on the radioactive element Cs-137, and can effectively improve the host's ability to resist heavy metal pollution such as Cu ²⁺ , Cd ²⁺ and Pb ²⁺ . Mycorrhizae play a positive role in promoting the stability of forest ecosystem and the transformation and transportation of energy. It is a special parasitic phenomenon in which plants and microorganisms achieve a high degree of unity. A large number of mycorrhizal application studies at home and abroad have shown that mycorrhizal can significantly promote the growth of host plants, improve the quality of seedlings, promote the host's absorption of nitrogen, phosphorus, and potassium, enhance plant stress resistance, and improve the rhizosphere environment of host plants. Whether the beneficial effect of mycorrhizal can be fully exerted depends on the correct selection of mycorrhizal fungi and whether mycorrhizal fungi can effectively infect host plants. For different tree species, tree capacitance, bark relative expansion, peroxidase activity, polyphenol oxidase activity and phenylalanine ammonia lyase activity indicators are closely related to tree disease resistance or stress resistance. relation.

Contents of the invention

In view of the above-mentioned problems in the prior art, the purpose of the present invention is to design and provide a technical solution for improving the stress resistance of garden plants, which has the advantages of low cost, remarkable effect, natural environmental protection, and no toxic and side effects.

A described method for improving stress resistance of garden plants is characterized in that it comprises the following steps:

1) Bacteria solution preparation

Take the mycorrhizal spores according to the weight ratio: distilled water=1-3:4-6, mix and make the mycorrhizal spore suspension;

2) Mycorrhizal treatment

Plant seeds are germinated at 23-28°C, soaked in mycorrhizal spore suspension for 5-12 minutes when the seeds are 1/2 white, and then sowed into peat: nutrient soil volume ratio of 3-5:1 to cultivate soil, etc. After the seeds germinate for 60-80 days, inject 2-6ml/strain of mycorrhizal spore suspension with a syringe at a distance of 2-3cm from the root of the seedling, and then cover the injection site with cultivation soil and cultivate until 6-8 months later It can be transplanted to any soil for cultivation, and after transplanting, evenly spray 2-4L of mycorrhizal spore suspension per square meter of soil, and then cultivate normally.

The method for improving stress resistance of garden plants is characterized in that the germination temperature of plant seeds is 24-27°C, preferably 25-26°C; the soaking time is 6-10min, preferably 7-9min.

The method for improving stress resistance of garden plants is characterized in that the volume ratio of peat in the cultivation soil: nutrient soil is 4:1.

The method for improving the stress resistance of garden plants is characterized in that the injection time of the bacterial solution is 65-75 days after the seeds germinate, and the amount of the injected bacterial solution is 3-5ml/plant.

The method for improving the stress resistance of garden plants is characterized in that the transplanting time is 6-8 months after cultivation, and the spraying amount of the bacterial solution is 2.5-3L/㎡.

The above-mentioned mycorrhizal spores can be purchased directly from the market.

The above-mentioned method for improving the stress resistance of garden plants uses distilled water as a medium to prepare a suspended bacterial liquid with mycorrhizal spores, and through the treatment of the bacterial liquid of garden plant seeds and seedlings, it has the effect of promoting seed vitality and improving seedling mycorrhizae. Synthesis, promoting the growth of seedlings, improving the stress resistance of plants and other functions, can effectively resist the occurrence of various diseases, and has special effects on improving the stress resistance of saplings. It is natural and environmentally friendly, has no pollution to the environment, is safe to use, has remarkable effects, and is low in cost, and is suitable for application in planting, transplanting and cultivating garden plants.

Unless otherwise specified, the percentages involved in the present application documents are all weight percentages of pure substances.

detailed description

The beneficial effects of the present invention will be further described in conjunction with the embodiments of the present invention.

Example 1

According to the ratio of mycorrhizal fungal spores:distilled water=2:5, the mycorrhizal fungal spore suspension was prepared evenly, and then the volume ratio of peat:nutrient soil=4:1 was used to make the cultivation soil, and then the plant seeds were germinated at 23°C. When the seeds are 1/2 white, soak them in mycorrhizal spore suspension for 5 minutes, and then sow them into the cultivation soil. After the seeds germinate for 60 days, inject 6ml/strain of mycorrhizal fungi with a syringe at a place 3cm away from the root of the seedlings. The spore suspension, and then cover the injection site with cultivation soil, and transplant it to ordinary soil after cultivating for 6 months. After transplanting, spray 2L of mycorrhizal spore suspension evenly in each square meter of soil, and then cultivate normally.

Example 2

According to the ratio of mycorrhizal fungal spores:distilled water=3:5, the mycorrhizal fungal spore suspension is prepared by mixing evenly, and then the cultivation soil is made according to the volume ratio of peat:nutrient soil=3:1, and then the plant seeds are germinated at 28°C. When the seeds are 1/2 white, soak them in the mycorrhizal suspension for 12 minutes, and then sow them into the cultivation soil. After the seeds germinate for 80 days, inject 2ml/strain of mycorrhizal spores with a syringe at a place 3cm away from the root of the seedlings. Suspension, and then cover the injection site with cultivation soil, and transplant to ordinary soil after cultivation until August. After transplanting, spray 4L of mycorrhizal spore suspension evenly in each square meter of soil, and then cultivate normally.

Example 3

According to the ratio of mycorrhizal fungal spores: distilled water = 4:6, the mycorrhizal fungal spore suspension is made by mixing evenly, and then the cultivation soil is made according to the volume ratio of peat: nutrient soil = 4:1, and then the plant seeds are germinated at 24°C. When the seeds are 1/2 white, soak them in the mycorrhizal suspension for 6 minutes, and then sow them into the cultivation soil. After the seeds germinate for 65 days, inject 3ml/strain of mycorrhizal spores with a syringe at a place 3cm away from the root of the seedlings. Suspension, and then cover the injection site with cultivation soil, and transplant to ordinary soil after cultivation until July. After transplanting, spray 3L of mycorrhizal spore suspension evenly in each square meter of soil, and then cultivate normally.

Example 4

According to the ratio of mycorrhizal fungal spores: distilled water = 2:5, the mycorrhizal fungal spore suspension is made by mixing evenly, and then the cultivation soil is made according to the volume ratio of peat: nutrient soil = 5:1, and then the plant seeds are germinated at 25°C. When the seeds are 1/2 white, soak them in the mycorrhizal suspension for 7 minutes, and then sow them into the cultivation soil. After the seeds germinate for 70 days, inject 4ml/strain of mycorrhizal spores with a syringe at a place 3cm away from the roots of the seedlings. Suspension, and then cover the injection site with cultivation soil, and transplant to ordinary soil after cultivating for 6.5 months. After transplanting, spray 2.5L of mycorrhizal spore suspension evenly in each square meter of soil, and then cultivate normally.

Example 5

According to the ratio of mycorrhizal fungal spores:distilled water=2:5, the mycorrhizal fungal spore suspension was prepared evenly, and then the volume ratio of peat:nutrient soil=4:1 was used to make the cultivation soil, and then the plant seeds were germinated at 26°C. When the seeds are 1/2 white, soak them in the mycorrhizal suspension for 8 minutes, and then sow them into the cultivation soil. After the seeds germinate for 75 days, inject 5ml/strain of mycorrhizal spores with a syringe at a place 3cm away from the root of the seedlings. Suspension, and then cover the injection site with cultivation soil, and transplant it to ordinary soil after 7.5 months of cultivation. After transplanting, spray 3.5L of mycorrhizal spore suspension evenly in each square meter of soil, and then cultivate normally.

Example 6

According to the ratio of mycorrhizal spores: distilled water = 3:4, the mycorrhizal spore suspension is prepared by mixing evenly, and then the cultivation soil is made according to the volume ratio of peat: nutrient soil = 4:1, and then the plant seeds are germinated at 27°C. When the seeds are 1/2 white, soak them in the mycorrhizal suspension for 10 minutes, and then sow them into the cultivation soil. After the seeds germinate for 72 days, inject 3.5ml/strain of mycorrhizal fungi with a syringe at a place 3cm away from the root of the seedlings. Spore suspension, and then cover the injection site with cultivation soil, and transplant it to ordinary soil after cultivating for 6 months. After transplanting, spray 2.8L mycorrhizal spore suspension evenly in each square meter of soil, and then cultivate normally .

Further illustrate the beneficial effect of the present invention by experiment below.

Table 1 shows: embodiment 1-6 all has good promoting effect to sapling mycorrhizal synthesis and seedling growth, shows that using this mycorrhizal technology can improve the stress resistance of plant, strengthen its comprehensive physiological and biochemical ability, wherein, implement Example 1 is better than Example 2, Example 3 is better than Example 4, and Example 5 is better than Example 6.

Table 2 shows: embodiment 1-6 all has better promoting effect to seedling disease resistance-related enzyme activity, and wherein, embodiment 1 is better than embodiment 2, and embodiment 3 is better than embodiment 4, and embodiment 5 is better than Example 6 was significantly higher than that of the control group.

Claims (6)

1. A method for improving stress resistance of garden plants, characterized in that it may further comprise the steps: 1) Preparation of bacterial solution Take the mycorrhizal spores according to the weight ratio: distilled water=1-3:4-6, mix evenly to make the mycorrhizal spore suspension; 2) Mycorrhizal treatment Plant seeds are germinated at 23-28°C, soaked in mycorrhizal spore suspension for 5-12 minutes when the seeds are 1/2 white, and then sowed into peat: nutrient soil volume ratio of 3-5:1 to cultivate soil, etc. After the seeds germinate for 60-80 days, inject 2-6ml/strain of mycorrhizal spore suspension with a syringe at a distance of 2-3cm from the root of the seedling, and then cover the injection site with cultivation soil and cultivate until 6-8 months later It can be transplanted to any soil for cultivation, and after transplanting, spray 2-4L of mycorrhizal spore suspension evenly in each square meter of soil, and then cultivate normally. 2. A method for improving the stress resistance of garden plants as claimed in claim 1, characterized in that the germination temperature of the plant seeds is 24-27°C, and the soaking time is 6-10min. 3. A method for improving the stress resistance of garden plants as claimed in claim 1, characterized in that the germination temperature of the plant seeds is 25-26°C, and the soaking time is 7-9min. 4. A kind of method improving garden plant stress resistance as claimed in claim 1, is characterized in that cultivating peat in the soil: the nutrient soil volume ratio is 4:1. 5. A method for improving stress resistance of garden plants as claimed in claim 1, characterized in that the injection time of the bacterial solution is 65-75 days after the seeds germinate, and the amount of the injected bacterial solution is 3-5ml/strain. 6. A method for improving the stress resistance of garden plants as claimed in claim 1, characterized in that the transplanting time is after 6-8 months of cultivation, and the spraying amount of the bacterial solution is 2.5-3L/㎡.