CN102715004A - Method for improving stress resistance of landscape plants - Google Patents
Method for improving stress resistance of landscape plants Download PDFInfo
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- CN102715004A CN102715004A CN2012102206635A CN201210220663A CN102715004A CN 102715004 A CN102715004 A CN 102715004A CN 2012102206635 A CN2012102206635 A CN 2012102206635A CN 201210220663 A CN201210220663 A CN 201210220663A CN 102715004 A CN102715004 A CN 102715004A
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- mycorrhizal
- spore suspension
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 239000002689 soil Substances 0.000 claims abstract description 54
- 239000000725 suspension Substances 0.000 claims abstract description 37
- 238000002347 injection Methods 0.000 claims abstract description 18
- 239000007924 injection Substances 0.000 claims abstract description 18
- 239000003415 peat Substances 0.000 claims abstract description 11
- 239000007921 spray Substances 0.000 claims abstract description 5
- 235000015097 nutrients Nutrition 0.000 claims abstract 3
- 241000894006 Bacteria Species 0.000 claims description 47
- 239000012153 distilled water Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000007226 seed germination Effects 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- 230000035784 germination Effects 0.000 claims 1
- 238000002791 soaking Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 12
- 241000233866 Fungi Species 0.000 abstract description 8
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000002538 fungal effect Effects 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 32
- 230000008569 process Effects 0.000 description 13
- 230000035764 nutrition Effects 0.000 description 8
- 235000016709 nutrition Nutrition 0.000 description 8
- 238000009331 sowing Methods 0.000 description 7
- 208000035240 Disease Resistance Diseases 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- 230000018109 developmental process Effects 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 241001453380 Burkholderia Species 0.000 description 1
- 102000030523 Catechol oxidase Human genes 0.000 description 1
- 108010031396 Catechol oxidase Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 240000000950 Hippophae rhamnoides Species 0.000 description 1
- 235000003145 Hippophae rhamnoides Nutrition 0.000 description 1
- 241001233115 Paxillus involutus Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000168036 Populus alba Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000219492 Quercus Species 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 230000000767 anti-ulcer Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
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- 238000011081 inoculation Methods 0.000 description 1
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- 230000000050 nutritive effect Effects 0.000 description 1
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- 239000011574 phosphorus Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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- Cultivation Of Plants (AREA)
Abstract
一种提高园林植物抗逆性的方法，属于园林植物保护技术领域。包括以下步骤：1）制备菌根菌孢子悬浮液；2）植物种子在23-28℃下催芽，当种子露白1/2时在菌根菌悬浮液中浸泡5-12min，然后播种入泥炭：营养土体积比为3-5:1培育土壤中，等种子发芽60-80天后，再在离苗木根部2-3cm处用针筒注射2-6ml/株的菌根菌孢子悬浮液，然后用培育土壤覆盖压紧注射处，培养至6-8月后可移栽，移栽后再喷洒菌根菌孢子悬浮液。上述一种提高园林植物抗逆性的方法，具有促进种子活力、提高幼苗菌根的合成，促进苗木生长，提高植物的抗逆性等多种功效，能有效抵御各种病害的发生，对提高树苗抗逆性有特效。The invention discloses a method for improving stress resistance of garden plants, which belongs to the technical field of garden plant protection. It includes the following steps: 1) Prepare the mycorrhizal fungus spore suspension; 2) Germinate the plant seeds at 23-28°C, soak in the mycorrhizal fungal suspension for 5-12 minutes when the seeds are 1/2 white, and then sow into peat: The nutrient soil volume ratio is 3-5:1 in the cultivation soil, and after the seeds germinate for 60-80 days, inject 2-6ml/strain of mycorrhizal spore suspension with a syringe at a distance of 2-3cm from the root of the seedling, and then use Cultivate the soil to cover and compact the injection site, and transplant after 6-8 months of cultivation, and then spray the mycorrhizal spore suspension after transplanting. The above-mentioned method for improving the stress resistance of garden plants has multiple effects such as promoting seed vigor, improving the synthesis of seedling mycorrhiza, promoting seedling growth, and improving the stress resistance of plants, and can effectively resist the occurrence of various diseases. The saplings have special effects on stress resistance.
Description
Technical field
The invention belongs to ornamental plant resist technology field, be specially a kind of method that improves ornamental plant resistance.
Background technology
Mycorhiza bacterium (mycorrhizal fungi) is a parasymbiosis fungi that can form symbiont in the soil with the young tender part of plant rhizosphere, has resistance (comprising drought resistance, salt-resistance and disease resistance etc.), the promotion seedling growth that improves host plant and strengthens the host to Cd
2+Contamination resistance in heavy metal.Song Fu waits research to think by force, and the survival rate of Mycorrhizal afforestation can reach the once successful effect of afforestation on average more than 95%, is an indispensable biotechnology in the production of forestry.Tang Ming etc. investigate from the mycorrhizal fungi resource the many semiarid zones of NORTHWEST CHINA; Furtherd investigate from the drought resistance influence of mycorrhizal fungi plants such as sea-buckthorns; And inquired into from branch mycorhiza bacterium and improved the sick mechanism of willow antiulcer, promoted the development and utilization of China's mycorhiza resource to a certain extent.As a kind of typical ectotrophic mycorrhiza fungi,
Paxillus involutus(Batsch ex Fr.) Fr. distribution on the Northern Hemisphere is very general, and the inoculation back is than being easier to form mycorhiza.Because
P. involutusCan improve the anti-adversity of host plant effectively, and promote the growth of host plant, exist both at home and abroad
P. involutusThe development and utilization aspect carried out more research, and obtained bigger progress.Quoreshi and Khasa discover, when
P. involutusWith bacterium
Burkholderia cepaciaWhen inoculating together, can improve white poplar significantly
P. tremuloidesWith the face cream poplar
P. angustifoliaTo the absorption of nutritive elements such as N and P, thus the growth that improves root system.Researchs such as Garbaye think,
P. involutusThe mycorhiza that forms can improve significantly
Quercus petraeaWith
Q. roburThe drought-resistant ability of tree.In addition,
P. involutusRadioactive element Cs-137 is also had very strong absorption, and can improve the anti-Cu of host effectively
2+, Cd
2+And Pb
2+Ability Deng heavy metal pollution.Mycorhiza plays positive facilitation to the conversion transportation of the stable and energy of forest ecosystem, and it is a kind of special parasitism that plant and microorganism reach high unity.A large amount of mycorhiza application study both at home and abroad shows that mycorhiza can obviously promote the growth of host plant, improves seedling quality, promotes the absorption of host to nitrogen, phosphorus, potassium, and enhancement of plant resistance is improved host plant rhizospheric environment etc.Whether this useful influence of mycorhiza can be given full play to, and is decided by that can the correctness and the mycorrhizal fungi of mycorrhizal fungi selection effectively infect host plant.As far as different seeds, tree body capacitance, bark relative expansion degree, peroxidase activity, polyphenol oxidase activity and PAL activity index and trees disease resistance or resistance have confidential relation.
Summary of the invention
To the problems referred to above that exist in the prior art, the objective of the invention is to design provides a kind of technical scheme that improves the method for ornamental plant resistance, and it is low to have a cost, and effect is remarkable, natural environmental-protective, advantage such as without any side effects.
Described a kind of method that improves ornamental plant resistance is characterized in that may further comprise the steps:
1) bacterium liquid preparation
Take by weighing mycorhiza bacterium Bao ︰ distilled water=1-3:4-6 according to weight ratio, mix and process mycorhiza bacterium spore suspension;
2) Mycorrhizal is handled
Plant seed is 23-28 ℃ of following vernalization; When showing money or valuables one carries unintentionally, seed in mycorhiza bacterium spore suspension, soaks 5-12min 1/2 the time; Peat is gone in sowing then: nutrition soil volume ratio is that 3-5:1 cultivates in the soil, wait seed sprouting 60-80 days after, again at the mycorhiza bacterium spore suspension with needle cylinder injection 2-6ml/ strain from nursery stock root 2-3cm place; Compress injection place with cultivating the soil covering then; Be cultured to 6-8 and can transplant to any soil after the month and cultivate, evenly spray 2-4L mycorhiza bacterium spore suspension in the Yong Mei ㎡ soil again after the transplanting, after this normal cultivation gets final product.
Described a kind of method that improves ornamental plant resistance is characterized in that the plant seed germination temperature is 24-27 ℃, preferred 25-26 ℃; Soak time is 6-10min, preferred 7-9min.
Described a kind of method that improves ornamental plant resistance is characterized in that cultivating peat in the soil: nutrition soil volume ratio is 4:1.
Described a kind of method that improves ornamental plant resistance is characterized in that injecting the bacterium liquid time and is behind the seed sprouting 65-75 days, and injection bacterium liquid measure is the 3-5ml/ strain.
Described a kind of method that improves ornamental plant resistance is characterized in that transplanting time is to cultivate 6-8 after the month, and bacterium liquid sprinkling amount is 2.5-3L/ ㎡.
Above-mentioned mycorhiza bacterium spore can directly be buied from market.
Above-mentioned a kind of method that improves ornamental plant resistance is a medium with distilled water, is prepared into suspension bacteria liquid with mycorhiza bacterium spore; Bacterium liquid through to ornamental plant seed and seedling is handled; Have and promote the synthetic of seed vitality, raising seedling mycorhiza, promotion seedling growth, the multiple efficacies such as resistance of raising plant; Can effectively resist the generation of various diseases, special efficacy arranged improving sapling resistance.Its natural environmental-protective does not have any pollution to environment, and safe in utilization, and effect is remarkable, and is with low cost, is adapted at the ornamental plant plantation, transplants and cultivates going up using.
The percentage composition that relates in the present specification unless otherwise indicated, other the weight percentage that is pure material.
Embodiment
Combine embodiments of the invention at present, further specify beneficial effect of the present invention.
Embodiment 1
Evenly process mycorhiza bacterium spore suspension according to mycorhiza bacterium Bao ︰ distilled water=2:5 mixed, again peat by volume: nutrition soil=4:1 processes cultivation soil, then with plant seed 23 ℃ of following vernalization; Put into mycorhiza bacterium spore suspension 1/2 the time and soak 5min when seed shows money or valuables one carries unintentionally; Sowing goes into to cultivate in the soil then, waits seed sprouting after 60 days, again at the mycorhiza bacterium spore suspension with needle cylinder injection 6ml/ strain from nursery stock root 3cm place; And then cover and compress injection place with cultivating soil; Transplant to normal soil after being cultured to June, again with evenly spraying 2L mycorhiza bacterium spore suspension in every ㎡ soil, after this normally cultivate after the transplanting.
Embodiment 2
Evenly process mycorhiza bacterium spore suspension according to mycorhiza bacterium Bao ︰ distilled water=3:5 mixed, again peat by volume: nutrition soil=3:1 processes cultivation soil, then with plant seed 28 ℃ of following vernalization; Put into mycorhiza bacterium suspension 1/2 the time and soak 12min when seed shows money or valuables one carries unintentionally; Sowing goes into to cultivate in the soil then, waits seed sprouting after 80 days, again at the mycorhiza bacterium spore suspension with needle cylinder injection 2ml/ strain from nursery stock root 3cm place; And then cover and compress injection place with cultivating soil; Transplant to normal soil after being cultured to August, again with evenly spraying 4L mycorhiza bacterium spore suspension in every ㎡ soil, after this normally cultivate after the transplanting.
Embodiment 3
Evenly process mycorhiza bacterium spore suspension according to mycorhiza bacterium Bao ︰ distilled water=4:6 mixed, again peat by volume: nutrition soil=4:1 processes cultivation soil, then with plant seed 24 ℃ of following vernalization; Put into mycorhiza bacterium suspension 1/2 the time and soak 6min when seed shows money or valuables one carries unintentionally; Sowing goes into to cultivate in the soil then, waits seed sprouting after 65 days, again at the mycorhiza bacterium spore suspension with needle cylinder injection 3ml/ strain from nursery stock root 3cm place; And then cover and compress injection place with cultivating soil; Transplant to normal soil after being cultured to July, again with evenly spraying 3L mycorhiza bacterium spore suspension in every ㎡ soil, after this normally cultivate after the transplanting.
Embodiment 4
Evenly process mycorhiza bacterium spore suspension according to mycorhiza bacterium Bao ︰ distilled water=2:5 mixed, again peat by volume: nutrition soil=5:1 processes cultivation soil, then with plant seed 25 ℃ of following vernalization; Put into mycorhiza bacterium suspension 1/2 the time and soak 7min when seed shows money or valuables one carries unintentionally; Sowing goes into to cultivate in the soil then, waits seed sprouting after 70 days, again at the mycorhiza bacterium spore suspension with needle cylinder injection 4ml/ strain from nursery stock root 3cm place; And then cover and compress injection place with cultivating soil; Transplant to normal soil after being cultured to 6.5 months, evenly spray 2.5L mycorhiza bacterium spore suspension in the Yong Mei ㎡ soil again after the transplanting, normally cultivation after this.
Embodiment 5
Evenly process mycorhiza bacterium spore suspension according to mycorhiza bacterium Bao ︰ distilled water=2:5 mixed, again peat by volume: nutrition soil=4:1 processes cultivation soil, then with plant seed 26 ℃ of following vernalization; Put into mycorhiza bacterium suspension 1/2 the time and soak 8min when seed shows money or valuables one carries unintentionally; Sowing goes into to cultivate in the soil then, waits seed sprouting after 75 days, again at the mycorhiza bacterium spore suspension with needle cylinder injection 5ml/ strain from nursery stock root 3cm place; And then cover and compress injection place with cultivating soil; Transplant to normal soil after being cultured to 7.5 months, evenly spray 3.5L mycorhiza bacterium spore suspension in the Yong Mei ㎡ soil again after the transplanting, normally cultivation after this.
Embodiment 6
Evenly process mycorhiza bacterium spore suspension according to mycorhiza bacterium Bao ︰ distilled water=3:4 mixed, again peat by volume: nutrition soil=4:1 processes cultivation soil, then with plant seed 27 ℃ of following vernalization; Put into mycorhiza bacterium suspension 1/2 the time and soak 10min when seed shows money or valuables one carries unintentionally; Sowing goes into to cultivate in the soil then, waits seed sprouting after 72 days, again at the mycorhiza bacterium spore suspension with needle cylinder injection 3.5ml/ strain from nursery stock root 3cm place; And then cover and compress injection place with cultivating soil; Transplant to normal soil after being cultured to June, again with evenly spraying 2.8L mycorhiza bacterium spore suspension in every ㎡ soil, after this normally cultivate after the transplanting.
Below further specify beneficial effect of the present invention through test.
Table 1 shows: embodiment 1-6 all has facilitation effect preferably to the synthetic seedling growth that reaches of sapling mycorhiza; Show that this Mycorrhizal technology of use can improve the resistance of plant; Strengthen its comprehensive Physiology and biochemistry ability, wherein, embodiment 1 is superior to embodiment 2; Embodiment 3 is superior to embodiment 4, and embodiment 5 is superior to embodiment 6.
Table 2 shows: embodiment 1-6 all has facilitation effect preferably to nursery stock disease resistance related enzyme activity, and wherein, embodiment 1 is superior to embodiment 2, and embodiment 3 is superior to embodiment 4, and embodiment 5 is superior to embodiment 6, all is significantly higher than control group.
Claims (5)
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210220663.5A CN102715004B (en) | 2012-06-29 | 2012-06-29 | A kind of method that improves ornamental plant resistance |
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| CN201210220663.5A CN102715004B (en) | 2012-06-29 | 2012-06-29 | A kind of method that improves ornamental plant resistance |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828618A (en) * | 2014-03-26 | 2014-06-04 | 兰州大学 | Method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi |
| CN108668794A (en) * | 2018-05-25 | 2018-10-19 | 浙江吉瑞建设有限公司 | A kind of cultural method of Multifunctional garden landscape plant |
| CN110731217A (en) * | 2018-07-18 | 2020-01-31 | 东北林业大学 | method for improving tree transplanting survival rate by inoculating mycorrhizal fungi |
| CN112740949A (en) * | 2020-12-22 | 2021-05-04 | 西施生态科技股份有限公司 | Cultivation method capable of effectively improving growth performance of stress-resistant arbor seedling plants |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103828618A (en) * | 2014-03-26 | 2014-06-04 | 兰州大学 | Method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi |
| CN108668794A (en) * | 2018-05-25 | 2018-10-19 | 浙江吉瑞建设有限公司 | A kind of cultural method of Multifunctional garden landscape plant |
| CN110731217A (en) * | 2018-07-18 | 2020-01-31 | 东北林业大学 | method for improving tree transplanting survival rate by inoculating mycorrhizal fungi |
| CN112740949A (en) * | 2020-12-22 | 2021-05-04 | 西施生态科技股份有限公司 | Cultivation method capable of effectively improving growth performance of stress-resistant arbor seedling plants |
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| CN102715004B (en) | 2016-05-11 |
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