CN102577790B - Simulation cultivation soil with target pathogens, and production method and application of simulation cultivation soil with target pathogens - Google Patents
Simulation cultivation soil with target pathogens, and production method and application of simulation cultivation soil with target pathogens Download PDFInfo
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- CN102577790B CN102577790B CN201210021606.4A CN201210021606A CN102577790B CN 102577790 B CN102577790 B CN 102577790B CN 201210021606 A CN201210021606 A CN 201210021606A CN 102577790 B CN102577790 B CN 102577790B
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- 239000002689 soil Substances 0.000 title claims abstract description 93
- 238000004088 simulation Methods 0.000 title claims abstract description 39
- 244000052769 pathogen Species 0.000 title claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 239000007787 solid Substances 0.000 claims abstract description 15
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 14
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 11
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 239000003814 drug Substances 0.000 claims abstract description 4
- 229930014626 natural product Natural products 0.000 claims abstract description 4
- 238000004382 potting Methods 0.000 claims abstract description 4
- 238000012216 screening Methods 0.000 claims abstract description 4
- 244000061176 Nicotiana tabacum Species 0.000 claims abstract 3
- 230000001954 sterilising effect Effects 0.000 claims description 27
- 238000004659 sterilization and disinfection Methods 0.000 claims description 27
- 239000000463 material Substances 0.000 claims description 18
- 241000233647 Phytophthora nicotianae var. parasitica Species 0.000 claims description 16
- 210000003608 fece Anatomy 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 15
- 235000019504 cigarettes Nutrition 0.000 claims description 14
- 239000010871 livestock manure Substances 0.000 claims description 14
- 239000011159 matrix material Substances 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 14
- 239000003337 fertilizer Substances 0.000 claims description 13
- 238000012545 processing Methods 0.000 claims description 11
- 241000244206 Nematoda Species 0.000 claims description 8
- 239000002131 composite material Substances 0.000 claims description 8
- 235000012054 meals Nutrition 0.000 claims description 8
- 241000195940 Bryophyta Species 0.000 claims description 7
- 238000009630 liquid culture Methods 0.000 claims description 7
- 239000003415 peat Substances 0.000 claims description 7
- 235000013339 cereals Nutrition 0.000 claims description 5
- 239000003864 humus Substances 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 4
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- 230000008569 process Effects 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 230000000443 biocontrol Effects 0.000 claims description 3
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- 230000035800 maturation Effects 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
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- 235000021307 Triticum Nutrition 0.000 claims description 2
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- 238000010298 pulverizing process Methods 0.000 claims description 2
- 241000208125 Nicotiana Species 0.000 abstract description 10
- 208000015181 infectious disease Diseases 0.000 abstract description 5
- 238000011081 inoculation Methods 0.000 abstract description 4
- 239000001963 growth medium Substances 0.000 abstract description 2
- 241001148142 Pectobacterium atrosepticum Species 0.000 abstract 2
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- 229940079593 drug Drugs 0.000 abstract 1
- 239000002054 inoculum Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 4
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Abstract
The invention discloses simulation cultivation soil with target pathogens, and a production method and application of the simulation cultivation soil with target pathogens. The cultivation soil produced by the method can be used for testing infection resistance of a tobacco variety to Pectobacterium atrosepticum, screening drugs or bio-control agents and natural products for controlling tobacco Pectobacterium atrosepticum. The production method includes: A, breaking pathogen culture solid; B, well mixing the broken pathogen culture solid with culture medium; and C, well mixing the mixture obtained in step B with base soil. Pathogen inoculants in the cultivation soil with target pathogens are stable in quality and controllable in amount, and the cultivation soil can be used for potting and can be subjected to pond application and scattering around tobacco plant roots to serve as artificial pathogenic soil used for field cultivation. By using the pathogen cultivation soil compared with other means, inoculation is simpler, the pathogens live longer, and infection to tobacco plants is more effective.
Description
Technical field
The invention belongs to agriculture technical field of microbe application, particularly a kind of simulation cultivating soil with target morbidity germ and preparation method thereof and application.
Background technology
Black shank (tobacco black shank) is the Major Diseases in World tobacco production.At present, in laboratory, greenhouse have adopt dull and stereotyped mycelia piece amplexiform (Wang Ge, Zheng little Bo, Lu Jiayun etc. the research of Yunnan Province's tobacco black shank bacterium Virulence. Agricultural University Of Nanjing's journal, 1997,20(4): 30-35), blend the tobacco black shank bacterium inoculation methods such as mycelia liquid or zoospore liquid irrigating root.Mycelia piece amplexiforms conventionally in rhizome portion, and germ is infected from the epidermis of rhizome junction; Mycelia liquid or zoospore liquid irrigating root usually people for causing some wounds, so that germ is infected from root.These two kinds of method complicated operations, time-consuming, are not generally suitable for land for growing field crops artificial infection.Field application more be the inoculation of cereal culture, after inoculation, germ can breed, survive a period of time on the grain culture medium not utilizing, ensured fully contacting of germ and cigarette strain rhizome portion, be conducive to infection process, but also there is greater risk, the germ that cereal is cultivated is easy to become the bait of its Antagonistic Fungi and causes germ to be cleared up, and germ quantity reduces, and can not effectively infect cigarette strain and falls ill.Therefore, be necessary that preparation is similar to Continuous Tobacco Cropping and causes balck shank that serious field soil occurs imitating field natural occurrence, to measure the medicament of the anti-perception of tobacco bred to balck shank, screening control black shank or biocontrol microorganisms, natural products.
Summary of the invention
The object of the present invention is to provide the simulation cultivating soil with target morbidity germ; Another object is to provide a kind of preparation method of this simulation cultivating soil; The 3rd object is to provide the application of this simulation cultivating soil.
The first object of the present invention is achieved in that described simulation cultivating soil comprises target Pathogen culture thing, cultivation base-material and base soil, and the volume ratio of described target Pathogen culture thing, cultivation base-material and base soil is 1:2 ~ 4:4 ~ 8.
Another object of the present invention is achieved in that described method comprises that culture is prepared, cultivation matrix is prepared and cultivating soil preparation, specifically comprises:
A, culture prepare, and the culture with black shank germ of inoculate and cultivate maturation are smashed or blended into pieces to the meal shape of particle diameter 1 ~ 3mm;
B, cultivation matrix preparation, good fragmentation culture particle is mixed 1:2 ~ 4 by volume with cultivation base-material, and stirring and evenly mixing is made cultivation matrix;
C, cultivating soil preparation, mix the cultivating soil made from black shank germ in 1:2 ~ 4 by cultivation matrix by volume with base soil.
The simulation cultivating soil with target morbidity germ described in the present invention's the 3rd object is achieved in that is measured the anti-perception of balck shank for tobacco bred; Or the medicament of screening control black shank or biocontrol microorganisms, natural products.The described simulation cultivating soil with target morbidity germ is as potting soil; Or the pool execute cigarette strain root system, spread fertilizer over the fields cigarette strain rhizome surrounding as artificial germ soil for field production.
The present invention, with respect to prior art, has the following advantages and effect:
1, in the simulation cultivating soil with target morbidity germ, germ quantity is stablized, infects more effective.Make the crude opium of planting with black shank germ that the simulation cultivating soil with target morbidity germ forming is similar to Continuous Tobacco Cropping self-assembling formation.Because cultivation matrix is through processing, miscellaneous bacteria amount is little, and the content of organic matter of cultivation matrix own is high, easily water suction, retention ability are strong, be conducive to the survival of germ, created good humidity environment simultaneously for infecting of germ, germ and host are long time of contact, infect chance of success larger, infect more effective;
2, infection process mode more approaches nature.Simulation cultivating soil with target morbidity germ can be directly used in potting soil, and germ can pass through rhizome portion, the strain of root invasion cigarette; Also can execute on the pool, land for growing field crops cigarette strain root system, replace former cultivating soil, form the soil moved in to improve the original with black shank germ, germ can pass through the strain of root invasion cigarette; Spread fertilizer over the fields cigarette strain rhizome surrounding, germ can be infected cigarette strain by rhizome portion; The pool is executed cigarette strain root system, is spread fertilizer over the fields cigarette strain rhizome surrounding simultaneously, and germ can pass through rhizome portion, the strain of root invasion cigarette.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but never in any form the present invention is limited, and any change or the improvement based on training centre of the present invention, done, all belong to protection scope of the present invention.
Described simulation cultivating soil comprises target Pathogen culture thing, cultivation base-material and base soil, and the volume ratio of described target Pathogen culture thing, cultivation base-material and base soil is 1:2 ~ 4:4 ~ 8.
The volume ratio of described target Pathogen culture thing, cultivation base-material and base soil is 1:2.5 ~ 3.5:5 ~ 7.
Described target Pathogen culture thing is solid culture or liquid culture; Described cultivation base-material is any one or more than one the mixture in the peat composed of rotten mosses, farmyard manure or cake fertilizer; Described base soil is any one or more than one the mixture in humus soil, mold or non-mold.
Described farmyard manure behaviour, poultry, excrement of animals, or crop stalk farmyard manure of making after fermentation maturity is processed.
Described mold is one or more the composite soil in paddy rice, wheat, beans, cereal, potato class proportion of crop planting soil.
The method of the described simulation cultivating soil with target morbidity germ, described method comprises that culture is prepared, cultivation matrix is prepared and cultivating soil preparation, specifically comprises:
It is the meal shape of the culture with black shank germ of inoculate and cultivate maturation being smashed or blended into pieces particle diameter 1 ~ 3mm that described culture is prepared;
Described cultivation matrix preparation is that good fragmentation culture particle is mixed 1:2 ~ 4 by volume with cultivation base-material, and stirring and evenly mixing is made cultivation matrix;
Described cultivating soil preparation is that cultivation matrix is mixed to the cultivating soil made from black shank germ in 1:2 ~ 4 by volume with base soil.
Described culture is liquid culture or solid culture; Described cultivation base-material is for becoming any one or more than one mixture in the peat composed of rotten mosses of particle diameter 1 ~ 3mm, the farmyard manure becoming thoroughly decomposed or cake fertilizer through pulverization process.
Described base soil is any one or more than one composite soil through 50 ~ 60 ℃ of high-temperature sterilizations, sterilization processing and in without humus soil, mold or the non-mold of nematode, and base soil is ground into the meal shape of particle diameter 1 ~ 3mm.
As an example of black shank germ simulation cultivating soil example, further illustrate the present invention below, other target germ simulation cultivating soils can adopt the inventive method preparation equally.
embodiment 1
By the farmyard manure after the fermentation of balck shank germ solid culture and human and animal excreta and humus soil by volume the ratio of 1:2:4 be mixed with simulation cultivating soil.First the balck shank germ solid culture of 1 part of volume is broken into 1 ~ 3mm meal shape, adds the farmyard manure after the human and animal excreta fermentation after 50 ~ 55 ℃ of high-temperature sterilizations of 2 parts of volumes, sterilization processing fully to mix, mix thoroughly; By 4 parts of volumes, the humus soil after 50 ~ 55 ℃ of sterilizations mixes the simulation cultivating soil obtaining with balck shank germ again.
embodiment 2
By balck shank germ solid culture and the peat composed of rotten mosses and wet rice cultivation soil by volume the ratio of 1:4:8 be mixed with simulation cultivating soil.First the balck shank germ solid culture of 1 part of volume is broken into 1 ~ 3mm meal shape, adds the peat composed of rotten mosses after 4 parts of volume water vapour sterilizations, sterilization processing fully to mix, mix thoroughly; Again 8 parts of volumes are mixed after 55 ~ 60 ℃ of sterilizations and without the wet rice cultivation soil of nematode to the simulation cultivating soil obtaining with balck shank germ.
embodiment 3
By balck shank germ solid culture and the cake fertilizer that becomes thoroughly decomposed and beans mold by volume the ratio of 1:3.5:7 be mixed with simulation cultivating soil.First the balck shank germ solid culture of 1 part of volume is broken into 1 ~ 3mm meal shape, adds the cake fertilizer becoming thoroughly decomposed after 60 ~ 65 ℃ of sterilizations of 3.5 parts of volumes, sterilization processing fully to mix, mix thoroughly; Again 7 parts of volumes are mixed after 50 ~ 55 ℃ of sterilizations and without the beans mold of nematode to the simulation cultivating soil obtaining with balck shank germ.
embodiment 4
By balck shank germ liquid culture, the crop stalk farmyard manure becoming thoroughly decomposed and non-mold by volume the ratio of 1:2.5:4 be mixed with simulation cultivating soil.First by the balck shank germ solid culture of 1 part of volume, add the cake fertilizer that becomes thoroughly decomposed (being crushed to particle diameter 1 ~ 3mm) after 50 ~ 55 ℃ of sterilizations of 2.5 parts of volumes, sterilization processing fully to mix, mix thoroughly; Again 4 parts of volumes are mixed to the simulation cultivating soil obtaining with balck shank germ after 55 ~ 60 ℃ of sterilizations and without the non-work soil of nematode.
embodiment 5
By balck shank germ liquid culture, the poultry manure farmyard manure becoming thoroughly decomposed, non-mold by volume the ratio of 1:2.5:4 be mixed with simulation cultivating soil.First by the balck shank germ solid culture of 1 part of volume, add the cake fertilizer that becomes thoroughly decomposed (being crushed to particle diameter 1 ~ 3mm) after 60 ~ 65 ℃ of sterilizations of 2.5 parts of volumes, sterilization processing fully to mix, mix thoroughly; Again 4 parts of volumes are mixed after 50 ~ 55 ℃ of sterilizations and without the non-mold of nematode to the simulation cultivating soil obtaining with balck shank germ.
embodiment 6
The composite cultivation base-material that balck shank germ liquid culture, the peat composed of rotten mosses and farmyard manure are formed, non-the mold by volume ratio of 1:3:5 are mixed with simulation cultivating soil.First by the balck shank germ solid culture of 1 part of volume, add the above-mentioned composite cultivation base-material (being crushed to particle diameter 1 ~ 3mm) after 50 ~ 55 ℃ of sterilizations of 3 parts of volumes, sterilization processing fully to mix, mix thoroughly; Again 5 parts of volumes are mixed to the simulation cultivating soil obtaining with balck shank germ after 50 ~ 55 ℃ of sterilizations and without the non-work soil of nematode.
embodiment 7
The composite cultivation base-material that balck shank germ liquid culture, the peat composed of rotten mosses and cake fertilizer are formed, non-the mold by volume ratio of 1:3:6 are mixed with simulation cultivating soil.First by the balck shank germ solid culture of 1 part of volume, add the above-mentioned composite cultivation base-material (being crushed to particle diameter 1 ~ 3mm) after 50 ~ 55 ℃ of sterilizations of 3 parts of volumes, sterilization processing fully to mix, mix thoroughly; Again 6 parts of volumes are mixed to the simulation cultivating soil obtaining with balck shank germ after 50 ~ 55 ℃ of sterilizations and without the potato class mold of nematode.
Claims (5)
1. the simulation cultivating soil with target morbidity germ, comprises target Pathogen culture thing, cultivation base-material and base soil, it is characterized in that the volume ratio of described target Pathogen culture thing, cultivation base-material and base soil is 1:2 ~ 4:4 ~ 8; The preparation method of the described simulation cultivating soil with target morbidity germ comprises that culture is prepared, cultivation matrix is prepared and cultivating soil preparation, specifically comprises:
A, culture prepare, and the culture with black shank germ of inoculate and cultivate maturation are smashed or blended into pieces to the meal shape of particle diameter 1 ~ 3mm; Described culture is solid culture or liquid culture;
B, cultivation matrix preparation, good fragmentation culture particle is mixed 1:2 ~ 4 by volume with the cultivation base-material after more than 50 ℃ high-temperature sterilization, sterilization processing, and stirring and evenly mixing is made cultivation matrix; Described cultivation base-material is for becoming the farmyard manure becoming thoroughly decomposed of particle diameter 1 ~ 3mm and/or the mixture of cake fertilizer or the peat composed of rotten mosses and the farmyard manure becoming thoroughly decomposed and/or cake fertilizer through pulverization process; Described farmyard manure behaviour, poultry, excrement of animals, the farmyard manure that crop stalk is made after fermentation maturity is processed;
C, cultivating soil preparation, mix the cultivating soil made from black shank germ in 1:2 ~ 4 by cultivation matrix by volume with base soil; Described base soil is any one or more than one composite soil through 50 ~ 60 ℃ of high-temperature sterilizations, sterilization processing and in without humus soil, mold or the non-mold of nematode, and base soil is ground into the meal shape of particle diameter 1 ~ 3mm.
2. the simulation cultivating soil with target morbidity germ according to claim 1, is characterized in that the volume ratio of described target Pathogen culture thing, cultivation base-material and base soil is 1:2.5 ~ 3.5:5 ~ 7.
3. the simulation cultivating soil with target morbidity germ according to claim 1, is characterized in that described mold is one or more the composite soil in paddy rice, wheat, beans, cereal, potato class proportion of crop planting soil.
4. described in claim 1 ~ 3 any one, the simulation cultivating soil with target morbidity germ is measured the anti-perception of balck shank for tobacco bred; Or the medicament of screening control black shank or biocontrol microorganisms, natural products.
5. described in claim 1 ~ 3 any one, with the simulation cultivating soil of target morbidity germ, be used as potting soil; Or the pool execute cigarette strain root system, spread fertilizer over the fields cigarette strain rhizome surrounding as artificial germ soil for field production.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210021606.4A CN102577790B (en) | 2012-01-31 | 2012-01-31 | Simulation cultivation soil with target pathogens, and production method and application of simulation cultivation soil with target pathogens |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210021606.4A CN102577790B (en) | 2012-01-31 | 2012-01-31 | Simulation cultivation soil with target pathogens, and production method and application of simulation cultivation soil with target pathogens |
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| CN102577790A CN102577790A (en) | 2012-07-18 |
| CN102577790B true CN102577790B (en) | 2014-04-30 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102786350A (en) * | 2012-08-08 | 2012-11-21 | 郑红初 | Seedling raising matrix and preparation method thereof |
| CN104291892B (en) * | 2014-09-18 | 2016-05-25 | 云南省烟草农业科学研究院 | The female soil of a kind of control black shank and preparation and using method |
| CN113966704A (en) * | 2021-10-27 | 2022-01-25 | 云南省烟草公司大理州公司 | Black shank induction morbidity method for potted tobacco leaves of Honghuadajinyuan variety |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101381685A (en) * | 2008-10-28 | 2009-03-11 | 云南省烟草科学研究所 | A kind of preparation method of tobacco black shank inoculum |
| CN101671720A (en) * | 2009-09-22 | 2010-03-17 | 云南省烟草农业科学研究院 | Physiological race identification method of tobacco phytophthora parasitica in disease nursery |
| CN101914454A (en) * | 2010-08-12 | 2010-12-15 | 云南省烟草农业科学研究院 | Simple and convenient separation method for phytophthora parasitica var nicotianae |
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