CN102550284B - Edible mushroom culturing method - Google Patents
Edible mushroom culturing method Download PDFInfo
- Publication number
- CN102550284B CN102550284B CN201110459395.8A CN201110459395A CN102550284B CN 102550284 B CN102550284 B CN 102550284B CN 201110459395 A CN201110459395 A CN 201110459395A CN 102550284 B CN102550284 B CN 102550284B
- Authority
- CN
- China
- Prior art keywords
- solid
- production
- strains
- liquid
- edible
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 10
- 235000001674 Agaricus brunnescens Nutrition 0.000 title description 22
- 238000012258 culturing Methods 0.000 title 1
- 239000007787 solid Substances 0.000 claims abstract description 47
- 241000233866 Fungi Species 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000004519 manufacturing process Methods 0.000 claims abstract description 21
- 239000001963 growth medium Substances 0.000 claims abstract description 11
- 239000003381 stabilizer Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000002609 medium Substances 0.000 claims abstract description 6
- 239000000706 filtrate Substances 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 239000003795 chemical substances by application Substances 0.000 claims abstract 7
- 239000013587 production medium Substances 0.000 claims abstract 2
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 235000013312 flour Nutrition 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- 229930003451 Vitamin B1 Natural products 0.000 claims description 5
- 235000013527 bean curd Nutrition 0.000 claims description 5
- 229960003495 thiamine Drugs 0.000 claims description 5
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 5
- 239000011691 vitamin B1 Substances 0.000 claims description 5
- 235000010374 vitamin B1 Nutrition 0.000 claims description 5
- 239000002023 wood Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 240000006499 Flammulina velutipes Species 0.000 claims description 4
- 235000016640 Flammulina velutipes Nutrition 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 229920002774 Maltodextrin Polymers 0.000 claims description 3
- 239000005913 Maltodextrin Substances 0.000 claims description 3
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 3
- 229940035034 maltodextrin Drugs 0.000 claims description 3
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims 7
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims 2
- 235000011941 Tilia x europaea Nutrition 0.000 claims 2
- 229910052602 gypsum Inorganic materials 0.000 claims 2
- 239000010440 gypsum Substances 0.000 claims 2
- 239000004571 lime Substances 0.000 claims 2
- 241000894007 species Species 0.000 claims 2
- 240000000599 Lentinula edodes Species 0.000 claims 1
- 235000001715 Lentinula edodes Nutrition 0.000 claims 1
- 244000252132 Pleurotus eryngii Species 0.000 claims 1
- 235000001681 Pleurotus eryngii Nutrition 0.000 claims 1
- 239000000047 product Substances 0.000 abstract description 10
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 description 11
- 230000001580 bacterial effect Effects 0.000 description 10
- 235000019738 Limestone Nutrition 0.000 description 7
- PASHVRUKOFIRIK-UHFFFAOYSA-L calcium sulfate dihydrate Chemical compound O.O.[Ca+2].[O-]S([O-])(=O)=O PASHVRUKOFIRIK-UHFFFAOYSA-L 0.000 description 7
- 238000005243 fluidization Methods 0.000 description 7
- 239000006028 limestone Substances 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 238000013467 fragmentation Methods 0.000 description 6
- 238000006062 fragmentation reaction Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
- 238000012364 cultivation method Methods 0.000 description 5
- 241000234427 Asparagus Species 0.000 description 4
- 235000005340 Asparagus officinalis Nutrition 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 239000002893 slag Substances 0.000 description 4
- 230000035800 maturation Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229960001212 bacterial vaccine Drugs 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000003086 food stabiliser Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
本发明公开了一种食用菌培养方法，包括：将食用菌菌种接种到固体培养基中，于20-26℃培养25-40天，制得固体菌种；将稳定剂加入水或PDA液体培养基中，混匀，制得液化剂；将所述的固体菌种加入所述的液化剂中，搅拌，过滤，取滤液，制得液体菌种；将液体菌种接种到生产培养基中进行生产培养，得到食用菌。本发明在食用菌培养过程中，利用液化剂对固体菌种进行液体化，之后再接种进行生产培养，现用现做，操作简便，能减轻劳动强度，较好地控制菌种用量，利于标准化生产；可以使分散种植者的产品质量稳定，形成区域性规模生产；同时，能够有效控制污染，减少人工和原料浪费，提高生产效益和产品质量。The invention discloses a method for cultivating edible fungi, which comprises: inoculating edible fungus strains into a solid medium and cultivating them at 20-26°C for 25-40 days to obtain solid strains; adding a stabilizer to water or PDA liquid In the culture medium, mix well to prepare the liquefaction agent; add the solid strain to the liquefaction agent, stir, filter, and take the filtrate to obtain the liquid strain; inoculate the liquid strain into the production medium Carry out production culture to obtain edible fungus. In the process of cultivating edible fungi, the present invention uses a liquefaction agent to liquefy solid strains, and then inoculates them for production and cultivation. It is ready-to-use and ready-to-use, easy to operate, can reduce labor intensity, better control the amount of strains, and facilitates standardization Production; it can stabilize the product quality of scattered growers and form regional scale production; at the same time, it can effectively control pollution, reduce labor and raw material waste, and improve production efficiency and product quality.
Description
Technical field
The present invention relates to Edible Fungi field, relate in particular to a kind of cultivation method for edible mushroom.
Background technology
China is Edible Fungi big country, and edible mushroom annual production accounts for world's total amount 75% more than, reaches 2,000 ten thousand tons.Edible Fungi is mainly from Chinese countryside peasant's hand labour, practitioner surpasses 2,500 ten thousand people, main employing is the solid edible spawn cultivation method lagging behind in production at present, production process is: first in the medium that routine is produced kind, put into original seed, be the solid spawn of production use under proper temperature after cultivating maturation; Then in wooden airtight casing, the bacterium bag after solid spawn and sterilizing is put into simultaneously, used medicine suffocating sterilization, manually after inoculation, complete production operation and cultivate cultivation.During manual inoculation, due to tank-volumes restriction, the bacterium bag quantity of at every turn putting into is no more than 100 bags conventionally.
The advantage of above-mentioned cultivating method for edible fungi is: manufacturing requirements is not high, and technique is simple, and equipment investment is little, and method is easily grasped.But also there are many deficiencies in the method, specifically comprises: (1) edible mushroom belongs to seasonal product, and output is large, the concentration of production; But casing is less, in case, putting bag, medicament fumigating, inoculation, bag taking etc. need operate repeatedly, and hand labor intensity is large, inefficiency; (2) bacterial classification repeats switching breeding, easily makes " original seed " to lose physiologically active, causes product quality inconsistent; (3) medicament fumigating sterilizing in wooden case, contaminated environment, and can bring very big harm to operator's health; (4) medicament fumigating sterilizing and manual inoculation solid spawn in solid spawn making, wooden case, the mode of production falls behind, way of peasant, production is without standard; A quality, product is unstable.
Visible, bacterial classification is made and used is the important step in Edible Fungi, is the key that affects product quality.
Summary of the invention
The invention provides a kind of cultivation method for edible mushroom, edible fungus solid spawn is carried out being used further to productive culture after fluidization, existing with now doing, easy and simple to handle, constant product quality, is beneficial to standardized production.
A cultivation method for edible mushroom, comprising:
(1) by edible bacterial vaccine inoculation in solid culture medium, cultivate 25-40 days in 20-26 ℃, make solid spawn;
(2) stabilizing agent is added in water or PDA liquid nutrient medium, mix, make liquefier;
(3) described solid spawn is added in described liquefier, stir, filter, get filtrate, make liquid spawn;
(4) liquid-spawn inoculation is carried out to productive culture in productive culture base, obtain edible mushroom.
In step (1), described edible mushroom can be Asparagus, mushroom or Xingbao mushroom.
Described edible fungus species is that the mother of edible mushroom plants or original seed.
Described solid culture medium can adopt conventional edible mushroom solid culture medium.
For bacterial classification can be grown better, preferably, in weight portion, described solid culture medium comprises: wood fragments bits 45-50, corn flour 25-30, wheatfeed 15-20, peptone 5-8, land plaster 1-2, pulverized limestone 1-2, water 120-150;
Or, comprising: corncob 55-65, corn flour 15-25, wheatfeed 10-15, bean curd ground-slag 15-20, land plaster 1-2, pulverized limestone 1-2, water 110-130.
Wherein, the particle diameter of described wood fragments bits, corn flour, wheatfeed, peptone, land plaster, pulverized limestone, corncob or bean curd ground-slag is preferably 30-60 order.
Described solid spawn can be placed 1-2 week at normal temperatures, under low temperature, can place the several months.During use, select and send out full mycelia, fine and close dense white kind bottle.
In step (2), described stabilizing agent can adopt conventional food stabilizer, as colloid, dextrin, sugar ester etc.
Preferably, described stabilizing agent is one or both in polyvinyl alcohol and maltodextrin.Stabilizing agent can make that the solid spawn after fragmentation is particle stabilized to be suspended in liquefier, makes bacterial concentration even up and down, is consistent, and prevents the phenomenons such as caking.Use stabilizing agent can effectively improve the quality of liquid spawn, while guaranteeing inoculation liquid spawn, inoculum concentration consumption is accurate, realizes standardized production, thereby the bacterium product quality of growth is consistent etc.
In every liter of liquefier, the addition of described stabilizing agent is preferably 1-10g.
Preferably, in described liquefier, also contain one or more in white sugar, peptone and vitamin B1.
More preferably, in every liter of liquefier, the content of white sugar is 5-20g, and the content of peptone is 3-8g, and the content of vitamin B1 is 1 μ g-2 μ g.In liquefier, add appropriate white sugar, peptone and vitamin B1, the solid spawn can be after fragmentation provides essential nutrition, white sugar provides carbon source for bacterial classification, peptone is the nitrogenous source that is easy to absorption, both will have certain proportion, and vitamin B1 is the essential vitamin that edible mushroom self can not be synthetic, add these materials, the liquid environment that is beneficial to thalli growth can be provided, contribute to keep the activity of thalline.
In step (3), before described solid spawn adds liquefier, can first be divided into fritter, be convenient to follow-up stirring, fragmentation.
The weight ratio of described solid spawn and liquefier is preferably 1: 10-1: 20; More preferably 1: 10-1: 15.
By described stirring, can make solid spawn fragmentation, realize the fluidization of solid spawn.
In order to improve fragmentation efficiency, can adopt the agitator with propeller to carry out fragmentation processing to solid spawn.
Described mixing time is preferably 5-15 minute.
Described mixing speed is preferably 100-120 rev/min.
Described filtration can adopt 20-60 object screen pack.By filtering the liquid spawn that can obtain particle exquisiteness.
In step (4), described liquid spawn can be inoculated in productive culture base by steam type edible fungus inoculator, and easy to operate, inoculation efficiency is high.Notification number is that the utility model patent of CN201127201Y discloses this steam type edible fungus inoculator.
The edible mushroom obtaining after described productive culture, can gather in the crops its fruit body.
The present invention carries out, after fluidization, inoculating and carrying out productive culture to edible fungus solid spawn, has following beneficial effect:
(1) compare the directly productive culture mode of inoculation of existing solid spawn, adopt edible mushroom of the present invention to cultivate the result of use that can obtain liquid spawn with preparation method, there is inoculum concentration size capable of regulating, accurately easy to control, bacterial classification does not need repeatedly to transfer, be difficult for inactivation, and is beneficial to the advantages such as production in enormous quantities.
(2) compare existing mother is planted or original seed carries out the mode of productive culture after liquid culture again, in the present invention, after female kind or original seed are made solid culture medium, the preservation time is longer, while producing inoculation, carry out again fluidization processing, with liquefier, substitute the liquid nutrient medium that liquid fermentation tank is made, can be now little, easy and simple to handle with now doing, investing.
(3) can adopt special equipment to carry out the fluidization operation of solid spawn or the inoculation operation of liquid spawn, with machinery, substitute manually, reduce labor intensity, can greatly liberate rural area labour productive forces; And compare manual operations, more can control preferably bacterial classification consumption, be beneficial to standardized production, make to disperse grower's constant product quality, form regional large-scale production; Meanwhile, can ensure to produce and stablize, effectively control and pollute, reduce manually and wastage of material, improve productivity effect and product quality.
Embodiment
The production of embodiment 1 Asparagus
(1) preparation of solid culture medium: porphyrize after wood fragments bits, corn flour, wheatfeed, peptone, land plaster and pulverized limestone is dry, cross 40 mesh sieves; Get wood fragments bits 46g, corn flour 28g, wheatfeed 16g, peptone 6g, land plaster 1g, pulverized limestone 1g, add water 150ml, after mediation evenly, be encased in volume 250ml wide-mouth bottle, to 200ml place, compacting charge level, the middle pore of pricking several 10～15mm is to the bottle end; With double-deck brown paper and two rubber rings, tighten, with high pressure steam sterilization (121 ℃ of temperature keep 1.5 hours);
(2) preparation of solid spawn: after solid culture medium is cooling, the female kind of access Asparagus test tube 1/7 fritter, in 25 ℃ of cultivations 28 days, extremely in bottle, mycelia sent out completely, and pure white exquisiteness is maturation;
(3) preparation of liquefier: get white sugar 10g, peptone 3g, VB
11 μ g, polyvinyl alcohol 3g, be dissolved in 1000ml warm water; After dissolving, at 121 ℃, keep 40 minutes, cooling standby;
(4) solid spawn fluidization is made: get the cultured solid spawn of step (2) and under aseptic condition, be cut into irregular fritter, in the agitator of input with propeller, the liquefier that adds again step (3) to make, the ratio 1: 10 (weight ratio) of solid spawn and liquefier; Screw capping, turn on the power switch, start agitator, with the rotating speed rotations of 120 revs/min 10 minutes; Cross afterwards 40 object screen packs, get filtrate, obtain flammulina velutipes liquid strains fine and smooth, that contain solid culture matrix;
(5) under aseptic condition, flammulina velutipes liquid strains step (4) being made by conduit imports steam type edible fungus inoculator (referring to the utility model patent of notification number CN201127201Y), by steam type edible fungus inoculator, flammulina velutipes liquid strains is inoculated into and in productive culture base, carries out productive culture, cultivate 35 days, obtain Asparagus fruit body for 25 ℃.
The production of embodiment 2 mushrooms
(1) preparation of solid culture medium: porphyrize after corncob, corn flour, wheatfeed, bean curd ground-slag, land plaster and pulverized limestone is dry, cross 30 mesh sieves; Get corncob 65g, corn flour 25g, wheatfeed 10g, bean curd ground-slag 15g, land plaster 1g, pulverized limestone 2g, add water 130ml, after mediation evenly, be encased in volume 250ml wide-mouth bottle, to 200ml place, compacting charge level, the middle pore of pricking several 10～15mm is to the bottle end; With double-deck brown paper and two rubber rings, tighten, with high pressure steam sterilization (121 ℃ of temperature keep 2 hours);
(2) preparation of solid spawn: after solid culture medium is cooling, the female kind of access mushroom test tube 1/6 fritter, in 25 ℃ of cultivations 35 days, extremely in bottle, mycelia sent out completely, and pure white exquisiteness is maturation;
(3) preparation of liquefier: get white sugar 15g, peptone 5g, VB
12 μ g, maltodextrin 6g, be dissolved in the potato juice (raw material potato consumption 20%) of 1200ml; After dissolving, at 121 ℃, keep 40 minutes, cooling standby;
(4) solid spawn fluidization is made: get the cultured solid spawn of step (2) and under aseptic condition, be cut into irregular fritter, in the agitator of input with spiral, the liquefier that adds again step (3) to make, the ratio of solid spawn and liquefier is 1: 15 (weight ratio); Screw capping, turn on the power switch, start propeller mixer, with the rotating speed rotations of 120 revs/min 10 minutes; Cross afterwards 40 object screen packs, get filtrate, obtain mushroom liquid bacterial fine and smooth, that contain solid culture matrix;
(5) under aseptic condition, mushroom liquid bacterial step (4) being made by conduit imports steam type edible fungus inoculator (referring to the utility model patent of notification number CN201127201Y), by steam type edible fungus inoculator, mushroom liquid bacterial is inoculated in productive culture base and carries out productive culture, cultivate for 26 ℃ and within 120 days, obtain mushroom fruiting body.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110459395.8A CN102550284B (en) | 2011-12-31 | 2011-12-31 | Edible mushroom culturing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110459395.8A CN102550284B (en) | 2011-12-31 | 2011-12-31 | Edible mushroom culturing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102550284A CN102550284A (en) | 2012-07-11 |
| CN102550284B true CN102550284B (en) | 2014-03-12 |
Family
ID=46397943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110459395.8A Expired - Fee Related CN102550284B (en) | 2011-12-31 | 2011-12-31 | Edible mushroom culturing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102550284B (en) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972205A (en) * | 2012-12-10 | 2013-03-20 | 杨凌芝君生物科技有限责任公司 | Liquefying method and use method of solid strains of edible fungi and medicinal fungi |
| CN103416218A (en) * | 2013-07-21 | 2013-12-04 | 高青诚信菌业科技有限公司 | Efficient environment-friendly production method for needle mushrooms |
| CN103548564B (en) * | 2013-10-23 | 2015-05-13 | 上海市农业科学院 | Factory production method for shitake mushrooms |
| CN103548563B (en) * | 2013-10-23 | 2016-08-17 | 关岭百龙汇生物科技有限公司 | A kind of fragmentation processing method for germination bacterium liquid strain mycelium pellets |
| CN104106370B (en) * | 2014-06-04 | 2016-08-24 | 保山富群农业科技有限公司 | A kind of edible fungi liquid strain and production method thereof |
| CN105884438A (en) * | 2014-10-09 | 2016-08-24 | 遵义市明华福食用菌种植场 | Pleurotus eryngii culture medium and preparation method thereof |
| CN104557315B (en) * | 2015-01-20 | 2017-11-07 | 浙江大学 | Elegant precious mushroom liquefaction special bacteria culture medium and corresponding cultivation |
| CN104838886A (en) * | 2015-04-29 | 2015-08-19 | 吴中区胥口精益生物医药研究所 | Industrial edible fungi cultivation method |
| CN106258471A (en) * | 2015-05-18 | 2017-01-04 | 韦炳合 | A kind of Lentinus Edodes high yield cultivating method |
| CN104926511A (en) * | 2015-06-19 | 2015-09-23 | 桂林健成生物科技开发有限公司 | Application of post-sprout-harvesting seed husks/embryoid bodies and rhizome substrate in preparation of solid spawn of edible fungi |
| CN104926512A (en) * | 2015-06-19 | 2015-09-23 | 桂林健成生物科技开发有限公司 | Application of siraitia grosvenorii residue to production of solid spawn of edible fungi |
| CN105110856A (en) * | 2015-09-18 | 2015-12-02 | 临汾市尧都区杜怡霖种植专业合作社 | Lentinula edodes culture medium |
| CN105110977A (en) * | 2015-09-18 | 2015-12-02 | 临汾市尧都区杜怡霖种植专业合作社 | Cultivation medium used for increasing lentinacin in lentinus edodes |
| CN106495883A (en) * | 2016-11-04 | 2017-03-15 | 颍上县唐垛湖现代农业科技有限公司 | One kind is become thoroughly decomposed straw slag straw mushroom compost and preparation method thereof |
| CN108285873A (en) * | 2017-08-29 | 2018-07-17 | 上海雪榕生物科技股份有限公司 | A kind of preparation method of edible fungus solid spawn reduction liquid |
| CN109845586B (en) * | 2019-02-15 | 2021-05-11 | 宁德师范学院 | Preparation method of flammulina velutipes liquid strain containing high-number hypha growing points |
| CN109964736A (en) * | 2019-04-30 | 2019-07-05 | 容益(海南)农业开发有限公司 | A kind of preparation process and its application of edible mushroom suspension fluid strain |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85107763B (en) * | 1985-10-29 | 1987-07-29 | 北京市食品研究所 | Preparation method of mushroom strain |
| TWI308591B (en) * | 2001-11-16 | 2009-04-11 | Phytoculture Control Co Ltd | Organism-culturing apparatus and organism-culturing method |
| CN1673350A (en) * | 2005-01-26 | 2005-09-28 | 浙江大学 | A process for preparing ediable mushroom colloidal bacterial spawn |
| CN101063086A (en) * | 2006-04-24 | 2007-10-31 | 刘永昶 | Preparation method for special mother seed of edible mushroom liquid bacterial |
| CN101066029A (en) * | 2007-06-05 | 2007-11-07 | 浙江大学 | A method for preparing colloidal strains of edible fungi |
| CN101940128A (en) * | 2010-08-17 | 2011-01-12 | 哈尔滨工业大学（威海） | Method for culturing edible and medicinal fungal liquid seeds |
| CN102265754B (en) * | 2011-06-24 | 2013-01-30 | 郑雪平 | Pleurotus eryngii factory bottle cultivation method |
-
2011
- 2011-12-31 CN CN201110459395.8A patent/CN102550284B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102550284A (en) | 2012-07-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102550284B (en) | Edible mushroom culturing method | |
| CN103214302B (en) | Sawdust mushroom residue nutrition agent used for cultivating mozzie buster, and preparing method thereof | |
| CN102786333A (en) | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same | |
| CN1528117A (en) | A method for producing Hericium erinaceus and its special medium | |
| CN103798057A (en) | Tremella cultivation medium and tremella cultivation method | |
| CN101113409B (en) | Method for cultivating antler mythic fungus by using bacterium glass | |
| CN107937329B (en) | A method for improving the vitality of liquid bacteria | |
| CN110249912A (en) | A kind of method of Phellinus Phellinus factory vase planting | |
| CN103288529A (en) | Mushroom culture material and preparation method thereof | |
| CN105036924A (en) | Edible fungus culture medium with tea leaf waste as main raw materials | |
| CN102239784A (en) | Microorganism production process | |
| CN101411289B (en) | A method for cultivating Flammulina velutipes by using ramie bone | |
| CN102948324A (en) | Method for producing edible mushroom by used biological packing | |
| CN107517737A (en) | A kind of continuous quick bacteria stick manufacture craft of edible mushroom | |
| CN107950288B (en) | Straw mushroom cultivation process | |
| CN101717309A (en) | Culture medium for straw rotting edible fungi solid strain and method for preparing solid strain | |
| CN103420726A (en) | Method for preparing black fungus cultivation material | |
| CN105379556A (en) | Industrial pleurotus erygii cultivation method adopting temperature-controlled fruiting management technique | |
| CN102786334A (en) | Culture medium for culturing edible fungus production mother seeds | |
| CN103975763A (en) | Agrocybe cylindracea cultivation method | |
| CN103704012A (en) | Pholiota nameko mushroom block, mushroom block production process and special device of production of mushroom block | |
| CN106747839A (en) | A kind of culture medium of edible fungus, its preparation method and application | |
| CN110235698A (en) | A kind of cultural method of Antrodia camphorata | |
| CN110036829A (en) | A kind of preparation method of oyster mushroom solid liquefaction strain | |
| CN106431658B (en) | Oyster mushroom culture medium and oyster mushroom cultivation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140312 Termination date: 20181231 |