CN110235698A - A kind of cultural method of Antrodia camphorata - Google Patents
A kind of cultural method of Antrodia camphorata Download PDFInfo
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- CN110235698A CN110235698A CN201910670519.3A CN201910670519A CN110235698A CN 110235698 A CN110235698 A CN 110235698A CN 201910670519 A CN201910670519 A CN 201910670519A CN 110235698 A CN110235698 A CN 110235698A
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- 241001486992 Taiwanofungus camphoratus Species 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title claims abstract description 36
- 240000007313 Tilia cordata Species 0.000 claims abstract description 51
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 241000386927 Cinnamomum micranthum f. kanehirae Species 0.000 claims abstract description 23
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 239000007921 spray Substances 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 12
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- 238000011534 incubation Methods 0.000 claims abstract description 11
- 238000011081 inoculation Methods 0.000 claims abstract description 11
- 238000012545 processing Methods 0.000 claims abstract description 9
- 240000007591 Tilia tomentosa Species 0.000 claims abstract description 8
- 239000003755 preservative agent Substances 0.000 claims abstract description 8
- 230000002335 preservative effect Effects 0.000 claims abstract description 8
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 5
- 238000011161 development Methods 0.000 claims abstract description 4
- 230000018109 developmental process Effects 0.000 claims abstract description 4
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 4
- 230000035784 germination Effects 0.000 claims abstract description 4
- 238000005286 illumination Methods 0.000 claims abstract description 4
- 238000012797 qualification Methods 0.000 claims abstract description 4
- 239000008399 tap water Substances 0.000 claims abstract description 4
- 235000020679 tap water Nutrition 0.000 claims abstract description 4
- 241000723347 Cinnamomum Species 0.000 claims description 43
- 241001237160 Kallima inachus Species 0.000 claims description 40
- 239000000843 powder Substances 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 239000001963 growth medium Substances 0.000 claims description 19
- 229920001817 Agar Polymers 0.000 claims description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 17
- 244000061456 Solanum tuberosum Species 0.000 claims description 17
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 17
- 239000008272 agar Substances 0.000 claims description 17
- 239000008103 glucose Substances 0.000 claims description 17
- 235000012015 potatoes Nutrition 0.000 claims description 17
- 239000006286 aqueous extract Substances 0.000 claims description 16
- 238000009835 boiling Methods 0.000 claims description 14
- 235000015097 nutrients Nutrition 0.000 claims description 14
- 239000002994 raw material Substances 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 5
- 239000002893 slag Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000002390 rotary evaporation Methods 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 239000012188 paraffin wax Substances 0.000 claims description 3
- 238000004804 winding Methods 0.000 claims description 3
- 235000008429 bread Nutrition 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 241000233866 Fungi Species 0.000 abstract description 4
- 239000004615 ingredient Substances 0.000 abstract description 3
- 238000004904 shortening Methods 0.000 abstract description 2
- 150000003648 triterpenes Chemical class 0.000 description 9
- 150000004676 glycans Chemical class 0.000 description 8
- 229920001282 polysaccharide Polymers 0.000 description 8
- 239000005017 polysaccharide Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000123370 Antrodia Species 0.000 description 3
- 241000219071 Malvaceae Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 239000000284 extract Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 241000723346 Cinnamomum camphora Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- VCUFZILGIRCDQQ-KRWDZBQOSA-N N-[[(5S)-2-oxo-3-(2-oxo-3H-1,3-benzoxazol-6-yl)-1,3-oxazolidin-5-yl]methyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C1O[C@H](CN1C1=CC2=C(NC(O2)=O)C=C1)CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F VCUFZILGIRCDQQ-KRWDZBQOSA-N 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 241000222383 Polyporales Species 0.000 description 1
- 241000907897 Tilia Species 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 231100000753 hepatic injury Toxicity 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000033 toxigenic Toxicity 0.000 description 1
- 230000001551 toxigenic effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of cultural methods of Antrodia camphorata, are related to edible and medical fungi technical field.It is characterized in that: a kind of cultural method of Antrodia camphorata, comprising the following steps: cinnamomum kanehirai is cut into linden by linden processing, after peeling, the surface of linden is polished flat, and using tap water at normal temperature spray linden 7 days or more, the linden after spray sent to Sterilization Kettle and sterilizes；Prepare liquid spawn；Linden inoculation；The linden of bacterium germination qualification is taken out in fruit body development management, removes freshness protection package and preservative film, is placed in 27-30 DEG C of temperature, cultivate 1 year or more in the mushroom house of humidity 80-90%, without illumination in incubation.The present invention has the advantages that shortening incubation time, nutritional ingredient are close with wild Antrodia camphorata.
Description
Technical field
The present invention relates to edible and medical fungi technical fields, more specifically, it relates to a kind of cultural method of Antrodia camphorata.
Background technique
Antrodia camphorata belongs to Aphyllophorales, Polyporaceae, antrodia karst, perennial gill fungus mushroom, the entitled Antrodia of Latin
Camphorata is the novel species just delivered by biochemical boundary nineteen ninety.Because being grown in the peculiar protection class plant ox in Taiwan
It gains the name on camphor tree, is precious rare medicinal fungi.Antrodia camphorata fructification is being that Taiwan indigenous peoples is used in early days
It treats the hepatic injury because insobriety is led to, the jewellery that solution is still drank after a night, is widely used in liver protecting and anti-oxidant, anti-now
It is scorching, antitumor etc..
The artificial cultivation method of Antrodia camphorata includes cinnamomum kanehirai cultivation basswood method, solid-state cultivation and solution fermentation at present.
Solid-state cultivation is that Antrodia camphorata strain is carried out Mycelium culture with space packet, and space includes fibre object, carbohydrate, five cereals
Class etc., incubation time about three months, the shortcomings that toxigenic capacity is higher, the breeding method are as follows: triterpenes content is low, and effect is poor, no
Conducive to popularization.Solution fermentation be using 500 liters even tonne or more liquid fermentation slot carry out strain liquid ferment to receive
Take mycelium；Incubation time is short, arrives fortnight for seven.The shortcomings that breeding method are as follows: it is distinctive that wild Antrodia camphorata can not be obtained
Triterpenes, effect is poor, is unfavorable for promoting.Cinnamomum kanehirai cultivation basswood method is to be using the original host's cinnamomum kanehirai of antrodia or other lindens
Culture medium cultivates Antrodia camphorata, can obtain the ingredient identical as wild Antrodia camphorata, effect is identical, avoids wild cinnamomum kanehirai illegal logging；
The shortcomings that breeding method are as follows: incubation time was long, at 3 years or more.
Summary of the invention
In view of the deficiencies of the prior art, the present invention intends to provide a kind of cultural method of Antrodia camphorata, tool
Have the advantages that shortening incubation time, nutritional ingredient are close with wild Antrodia camphorata.
To achieve the above object, the present invention provides the following technical scheme that
A kind of cultural method of Antrodia camphorata, comprising the following steps:
Linden processing, cuts into linden for cinnamomum kanehirai, after peeling, the surface of linden is polished flat, and uses tap water at normal temperature
Spray linden 7 days or more, the linden after spray sent to Sterilization Kettle and sterilizes；
Liquid spawn is prepared, Antrodia camphorata fructification is chosen, using bulk is cut into after alcohol disinfecting, by blocky Antrodia camphorata fructification
It is placed on the culture medium in culture dish, using the opening of paraffin sealing culture dish, is put into 26 ± 1 DEG C of constant incubator culture 28
± 2 days, blocky Antrodia camphorata fructification was removed after the completion of culture, culture medium and mycelia are cut into block, battalion is poured into culture tank
Nutrient solution is cooled to 25 ± 1 DEG C after sterilizing, block is put into 25 ± 1 DEG C of constant temperature cultures 12 days or more in culture tank, obtains liquid bacteria
Kind；
Linden inoculation, the linden after sterilization is cooled to room temperature, and linden is packed into the freshness protection package after disinfection, liquid spawn is sprayed
It spills to the surface of linden, is vacuumized in freshness protection package, seal the sack of freshness protection package, be sent into 27-30 DEG C of temperature, humidity 80-90%
Bacteria room standing four months or more；
The linden of bacterium germination qualification is taken out in fruit body development management, removes freshness protection package and preservative film, be placed in 27-30 DEG C of temperature,
It is cultivated 1 year or more in the mushroom house of humidity 80-90%, illumination is not necessarily in incubation.
By using above-mentioned technical proposal, on the one hand surface polishing facilitates the later period to shovel lower Antrodia camphorata, is on the other hand being inoculated with
When be easier to keep liquid spawn here；Linden is put in the prior art and is impregnated in water, the nutrition although being sufficiently humidified so as to, in linden
Substance has certain loss, and water can slowly foul.The present invention can both reduce battalion inside linden by the way of spray
The loss of substance is supported, water will not foul, and be sufficiently humidified so as to linden.Liquid spawn is the basis of Antrodia camphorata production, quality
Superiority and inferiority is directly related to the quality of culture.The present invention selects good Antrodia camphorata fructification, obtains pure bacterium using tissue isolation
Filament expands culture using nutrient solution in culture tank, and culture speed is fast, and mycelia is pure, quality is high.Liquid spawn is using sprinkling
Mode be inoculated in linden surface, do not need to punch on linden surface, inoculation efficiency is high and liquid spawn is evenly distributed.Using this
The cultural method of invention, Antrodia camphorata fruiting time can shorten incubation time about at 532 days or so compared with prior art, warp
It crosses the triterpenes tested in Antrodia camphorata and polysaccharide body is close with wild Antrodia camphorata, effect is good, is conducive to promote.
Further preferably, in the linden processing step, the water after spray linden is collected into simultaneously circulated sprinkling.
By using above-mentioned technical proposal, when spray, a part of nutriment on linden surface can be lost with shower water,
The nutriment that circulated sprinkling can be such that part is lost enters inside linden, reduces the loss of linden nutriment.
Further preferably, in the preparation liquid spawn step, the mass ratio of block and nutrient solution is (1.2~1.3):
20。
By using above-mentioned technical proposal, when block is very little, needed for the mycelia growth that the nutriment of nutrient solution is more than, make
At waste, when block is too many, the nutriment of nutrient solution is difficult to needed for meeting mycelia growth, and therefore, it is necessary to control blocks and battalion
The mass ratio of nutrient solution is (1.2~1.3): 20.
Further preferably, the raw material of the culture medium includes following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 18-23g；
Cinnamomum kanahirai hay bark powder 8-12g；
Cinnamomum kanehirai dead leaf powder 6-10g；
Water 1000g.
By using above-mentioned technical proposal, Cinnamomum kanahirai hay bark and dead leaf are the leftover pieces of processing, and being ground into after powdery can be again
Secondary utilization reduces waste；The primitive environment that can simulate Antrodia camphorata is added in culture medium in Cinnamomum kanahirai hay bark powder and cinnamomum kanehirai dead leaf powder,
Nutriment in Cinnamomum kanahirai hay bark and dead leaf can be such that mycelia preferably grows.
Further preferably, the raw material of the culture solution includes following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 0.6-1.0g；
The aqueous extract 8-12g of Cinnamomum kanahirai hay bark and dead leaf；
Water 1000g.
By using above-mentioned technical proposal, the aqueous extract of Cinnamomum kanahirai hay bark and dead leaf can extract a part of nutrients
Matter can simulate the primitive environment of Antrodia camphorata, grow mycelia faster and better.
Further preferably, the culture solution the preparation method is as follows: by peeling potatoes, be diced block, boiling is added
Continue to boil 15-20min after boiling, filter, glucose and agar are added into filtrate, obtains mixed liquor；By Cinnamomum kanahirai hay bark and dead leaf
It crushes, after mixing with distilled water according to the mass ratio of 3:10,2-3h, filtering and removing slag, rotation are extracted in water-bath under conditions of 60-70 DEG C
Turn to obtain the aqueous extract of Cinnamomum kanahirai hay bark and dead leaf after being concentrated by evaporation, is added in mixed liquor, stirs evenly, obtain culture solution.
By using above-mentioned technical proposal, the aqueous extract of Cinnamomum kanahirai hay bark and dead leaf can extract a part of nutrients
Matter can simulate the primitive environment of Antrodia camphorata, improve the main nutrient composition content in mycelium, give birth to mycelia faster and better
It is long.
Further preferably, in the linden inoculation step, the method that seals the sack of freshness protection package are as follows: sealed using band
Then the sack of freshness protection package, the winding of blend compounds band coat several layers preservative film on freshness protection package surface.
Linden is avoided to touch the external world using band, adhesive tape and freshness protection package triple seal by using above-mentioned technical proposal
Air reduces the probability of living contaminants, improves survival rate.
Further preferably, in the linden inoculation step, the inoculum concentration of liquid spawn is 5-8ml/dm2。
By using above-mentioned technical proposal, the inoculum concentration of liquid spawn is too low, can reduce the yield of Antrodia camphorata, liquid spawn
Inoculum concentration it is too high, the supply of the nutriment on linden surface not enough, is unfavorable for Antrodia camphorata growth.
In conclusion compared with prior art, the invention has the following advantages:
(1) cultural method of the invention is used, Antrodia camphorata fruiting time can contract compared with prior art about at 532 days or so
Short incubation time, close with wild Antrodia camphorata by the triterpenes and polysaccharide body tested in Antrodia camphorata, effect is good, is conducive to push away
Extensively；
(2) Cinnamomum kanahirai hay bark and dead leaf are the leftover pieces of processing, can be utilized again after being ground into powdery, and waste, cinnamomum kanehirai are reduced
The primitive environment that can simulate Antrodia camphorata, the nutrition in Cinnamomum kanahirai hay bark and dead leaf is added in culture medium in skin powder and cinnamomum kanehirai dead leaf powder
Substance can be such that mycelia preferably grows；
(3) cultural method of the invention is simple, it can be achieved that industrialization, standardized production reduce production to facilitate expanding production
Cost.
Specific embodiment
Below with reference to embodiment, the present invention will be described in detail.
A kind of embodiment 1: cultural method of Antrodia camphorata, comprising the following steps:
Cinnamomum kanehirai, is cut into 10 kilograms or so of linden, after peeling, the surface of linden is polished flat, and adopts by linden processing
It being sprayed linden 7 days with tap water at normal temperature, the linden after spray send to Sterilization Kettle and sterilizes, and 121 DEG C of sterilising temp, sterilization time 4h.
Liquid spawn is prepared, Antrodia camphorata fructification is chosen, using bulk is cut into after alcohol disinfecting, by blocky Antrodia camphorata
Entity is placed on the culture medium in culture dish, and culture medium is PDA culture medium, using the opening of paraffin sealing culture dish, is put into 26
± 1 DEG C of constant incubator culture 28 ± 2 days removes blocky Antrodia camphorata fructification, culture medium and mycelia is cut after the completion of culture
At block, nutrient solution being poured into culture tank, 25 ± 1 DEG C are cooled to after sterilizing, block is put into constant temperature 25 ± 1 in culture tank
DEG C culture 12 days, obtain liquid spawn, the mass ratio of block and nutrient solution is 1.2:20.
The raw material of culture solution includes following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 0.8g；
Water 1000g.
Culture solution the production method is as follows: by peeling potatoes, be diced block, continue to boil 15min, mistake after boiling boiling is added
Glucose and agar are added into filtrate, obtains culture solution for filter.
Linden inoculation, the linden after sterilization is cooled to room temperature, is carried out disinfection to the sack of freshness protection package, and linden loading is disappeared
In freshness protection package after poison, liquid spawn is sprayed to the surface of linden, the inoculum concentration of liquid spawn is 5ml/dm2, in freshness protection package
It vacuumizes, seals the sack of freshness protection package, be sent into 27-30 DEG C of temperature, the bacteria room of humidity 80-90% stands four months.
The linden of bacterium germination qualification is taken out in fruit body development management, removes freshness protection package and preservative film, is placed in temperature 27-
30 DEG C, cultivate 1 year or more in the mushroom house of humidity 80-90%, illumination is not necessarily in incubation.
Embodiment 2: a kind of cultural method of Antrodia camphorata, difference from example 1 is that, in linden processing step,
Water after spray linden is collected into simultaneously circulated sprinkling.
Embodiment 3: a kind of cultural method of Antrodia camphorata, difference from example 1 is that, the raw material of culture medium includes
Following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 18g；
Cinnamomum kanahirai hay bark powder 8g；
Cinnamomum kanehirai dead leaf powder 6g；
Water 1000g；
Culture medium the production method is as follows: by peeling potatoes, be diced block, continue to boil 15min after boiling boiling is added, filter,
Glucose and agar are added into filtrate, Cinnamomum kanahirai hay bark is crushed to 100 mesh or less and obtains Cinnamomum kanahirai hay bark powder, by cinnamomum kanehirai dead leaf
It is crushed to 100 mesh or less and obtains cinnamomum kanehirai dead leaf powder, Cinnamomum kanahirai hay bark powder and cinnamomum kanehirai dead leaf powder are added in mixture, is put into and goes out
Bacterium kettle pours into culture dish after autoclave sterilization, obtains culture medium.
Embodiment 4: a kind of cultural method of Antrodia camphorata, the difference is that, the raw material of culture medium includes with embodiment 3
Following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 23g；
Cinnamomum kanahirai hay bark powder 12g；
Cinnamomum kanehirai dead leaf powder 10g；
Water 1000g.
Embodiment 5: a kind of cultural method of Antrodia camphorata, difference from example 1 is that, the raw material of culture solution includes
Following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 0.6g；
The aqueous extract 8g of Cinnamomum kanahirai hay bark and dead leaf；
Water 1000g；
Culture solution the preparation method is as follows: by peeling potatoes, be diced block, continue to boil 15min after boiling boiling is added, filter,
Glucose and agar are added into filtrate, obtains mixed liquor；Cinnamomum kanahirai hay bark and dead leaf are crushed, Cinnamomum kanahirai hay bark and Cinnamomum kanahirai hay dead leaf
Mass ratio is 1:1, after being mixed with distilled water according to the mass ratio of 3:10, water-bath extraction 2h under conditions of 60 DEG C, and filtering and removing slag,
The aqueous extract of Cinnamomum kanahirai hay bark and dead leaf is obtained after rotary evaporation concentration, is added in mixed liquor, stirs evenly, cultivated
Liquid.
Embodiment 6: a kind of cultural method of Antrodia camphorata, difference from example 1 is that, the raw material of culture solution includes
Following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 1.0g；
The aqueous extract 12g of Cinnamomum kanahirai hay bark and dead leaf；
Water 1000g；
Culture solution the preparation method is as follows: by peeling potatoes, be diced block, continue to boil 20min after boiling boiling is added, filter,
Glucose and agar are added into filtrate, obtains mixed liquor；Cinnamomum kanahirai hay bark and dead leaf are crushed, Cinnamomum kanahirai hay bark and Cinnamomum kanahirai hay dead leaf
Mass ratio is 1:1, after being mixed with distilled water according to the mass ratio of 3:10, water-bath extraction 3h under conditions of 70 DEG C, and filtering and removing slag,
The aqueous extract of Cinnamomum kanahirai hay bark and dead leaf is obtained after rotary evaporation concentration, is added in mixed liquor, stirs evenly, cultivated
Liquid.
Embodiment 7: a kind of cultural method of Antrodia camphorata, the difference is that, the raw material of culture solution includes with embodiment 3
Following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 0.6g；
The aqueous extract 8g of Cinnamomum kanahirai hay bark and dead leaf；
Water 1000g；
Culture solution the preparation method is as follows: by peeling potatoes, be diced block, continue to boil 15min after boiling boiling is added, filter,
Glucose and agar are added into filtrate, obtains mixed liquor；Cinnamomum kanahirai hay bark and dead leaf are crushed, Cinnamomum kanahirai hay bark and Cinnamomum kanahirai hay dead leaf
Mass ratio is 1:1, after being mixed with distilled water according to the mass ratio of 3:10, water-bath extraction 2h under conditions of 60 DEG C, and filtering and removing slag,
The aqueous extract of Cinnamomum kanahirai hay bark and dead leaf is obtained after rotary evaporation concentration, is added in mixed liquor, stirs evenly, cultivated
Liquid.
Embodiment 8: a kind of cultural method of Antrodia camphorata, difference from example 1 is that, in linden inoculation step,
The method for sealing the sack of freshness protection package are as follows: using the sack of band sealing freshness protection package, blend compounds band winding, then in freshness protection package table
Bread covers several layers preservative film, and preservative film coats 4 layers in the present embodiment.
Embodiment 9: a kind of cultural method of Antrodia camphorata, difference from example 1 is that, the matter of block and nutrient solution
Amount is than being 1.3:20.
Embodiment 10: a kind of cultural method of Antrodia camphorata, difference from example 1 is that, the inoculation of liquid spawn
Amount is 8ml/dm2。
Test method: using the method culture Antrodia camphorata in embodiment 1-10, making it grow to 2cm, and record cultivates the time,
And triterpenes content and polysaccharide body content in the Antrodia camphorata of testing example 1-10, the prior art and wild Antrodia camphorata.
Test result and analysis: the results are shown in Table 1 for cultivation time and composition test.As shown in Table 1, the training of embodiment 1
Educate the time is obviously shortened compared with prior art, and Cinnamomum kanahirai hay bark powder and cinnamomum kanehirai dead leaf powder is added in embodiment 3 in the medium, can
To simulate the primitive environment of Antrodia camphorata, mycelia can be made preferably to grow, further shorten and cultivate the time, embodiment 5 is being cultivated
The aqueous extract of Cinnamomum kanahirai hay bark and dead leaf is added in liquid, the cultivation time shortens 5 days, and Cinnamomum kanahirai hay is added in embodiment 7 in the medium
While bark powder and cinnamomum kanehirai dead leaf powder, the aqueous extract of Cinnamomum kanahirai hay bark and dead leaf is added in culture solution, cultivates time contracting
It is 11 days short, illustrate that improved culture medium and culture solution can cooperate with the fast-growth for promoting Antrodia camphorata.
And triterpenes content in the Antrodia camphorata of embodiment 1-10 and polysaccharide body content are higher than the cinnamomum kanehirai of the prior art
Cultivation basswood method, and it is close with the content of wild Antrodia camphorata.Cinnamomum kanahirai hay bark powder is added in the medium for embodiment 3 and cinnamomum kanehirai is withered
Ye Fen, triterpenes content improve 0.05, and polysaccharide body content improves 0.05；Cinnamomum kanahirai hay bark is added in embodiment 5 in culture solution
With the aqueous extract of dead leaf, triterpenes content improves 0.05, and polysaccharide body content improves 0.04；Embodiment 7 is in the medium
While Cinnamomum kanahirai hay bark powder and cinnamomum kanehirai dead leaf powder is added, the aqueous extract of addition Cinnamomum kanahirai hay bark and dead leaf in culture solution, three
Terpene content improves 0.14, and polysaccharide body content improves 0.13, illustrates that improved culture medium and culture solution can cooperate with rush
Nutrition is absorbed into Antrodia camphorata, improves triterpenes content and polysaccharide body content.
Table 1 cultivates time and composition test result
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-described embodiment,
All technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
For those of ordinary skill, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of cultural method of Antrodia camphorata, which comprises the following steps:
Linden processing, cuts into linden for cinnamomum kanehirai, after peeling, the surface of linden is polished flat, and uses tap water at normal temperature
Spray linden 7 days or more, the linden after spray sent to Sterilization Kettle and sterilizes；
Liquid spawn is prepared, Antrodia camphorata fructification is chosen, using bulk is cut into after alcohol disinfecting, by blocky Antrodia camphorata fructification
It is placed on the culture medium in culture dish, using the opening of paraffin sealing culture dish, is put into 26 ± 1 DEG C of constant incubator culture 28
± 2 days, blocky Antrodia camphorata fructification was removed after the completion of culture, culture medium and mycelia are cut into block, battalion is poured into culture tank
Nutrient solution is cooled to 25 ± 1 DEG C after sterilizing, block is put into 25 ± 1 DEG C of constant temperature cultures 12 days or more in culture tank, obtains liquid bacteria
Kind；
Linden inoculation, the linden after sterilization is cooled to room temperature, and linden is packed into the freshness protection package after disinfection, liquid spawn is sprayed
It spills to the surface of linden, is vacuumized in freshness protection package, seal the sack of freshness protection package, be sent into 27-30 DEG C of temperature, humidity 80-90% is supported
Bacterium room standing four months or more；
The linden of bacterium germination qualification is taken out in fruit body development management, removes freshness protection package and preservative film, be placed in 27-30 DEG C of temperature,
It is cultivated 1 year or more in the mushroom house of humidity 80-90%, illumination is not necessarily in incubation.
2. a kind of cultural method of Antrodia camphorata according to claim 1, which is characterized in that in the linden processing step,
Water after spray linden is collected into simultaneously circulated sprinkling.
3. a kind of cultural method of Antrodia camphorata according to claim 1, which is characterized in that the preparation liquid spawn step
In, the mass ratio of block and nutrient solution is (1.2 ~ 1.3): 20.
4. a kind of cultural method of Antrodia camphorata according to claim 1, which is characterized in that the raw material of the culture medium includes
Following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 18-23g；
Cinnamomum kanahirai hay bark powder 8-12g；
Cinnamomum kanehirai dead leaf powder 6-10g；
Water 1000g.
5. a kind of cultural method of Antrodia camphorata according to claim 1, which is characterized in that the raw material of the culture solution includes
Following components:
Peeled potatoes 200g；
Glucose 20g；
Agar 0.6-1.0g；
The aqueous extract 8-12g of Cinnamomum kanahirai hay bark and dead leaf；
Water 1000g.
6. a kind of cultural method of Antrodia camphorata according to claim 5, which is characterized in that the preparation method of the culture solution
It is as follows: by peeling potatoes, to be diced block, continue to boil 15-20min after boiling boiling is added, filter, glucose is added into filtrate
And agar, obtain mixed liquor；Cinnamomum kanahirai hay bark and dead leaf are crushed, after being mixed with distilled water according to the mass ratio of 3:10, in 60-70
2-3h is extracted in water-bath under conditions of DEG C, and filtering and removing slag obtain the aqueous extract of Cinnamomum kanahirai hay bark and dead leaf after rotary evaporation concentration, add
Enter into mixed liquor, stir evenly, obtains culture solution.
7. a kind of cultural method of Antrodia camphorata according to claim 1, which is characterized in that in the linden inoculation step,
The method for sealing the sack of freshness protection package are as follows: using the sack of band sealing freshness protection package, blend compounds band winding, then in freshness protection package table
Bread covers several layers preservative film.
8. a kind of cultural method of Antrodia camphorata according to claim 1, which is characterized in that in the linden inoculation step,
The inoculum concentration of liquid spawn is 5-8ml/dm2。
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| CN112314326A (en) * | 2020-10-28 | 2021-02-05 | 李昌发 | Method for cultivating antrodia camphorata by using basswood clinker |
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