CN102526283A

CN102526283A - Health-care medicine formula with functions of enhancing immunity, alleviating physical fatigue and resisting tumor

Info

Publication number: CN102526283A
Application number: CN2011103409280A
Authority: CN
Inventors: 李�杰; 施建耀
Original assignee: NINGBO HAIYI BIOTECHNOLOGY CO Ltd
Current assignee: NINGBO HAIYI BIOTECHNOLOGY CO Ltd
Priority date: 2011-11-02
Filing date: 2011-11-02
Publication date: 2012-07-04

Abstract

The invention discloses a health-care medicine with functions of enhancing immunity, alleviating physical fatigue and resisting tumor as well as a sugar pill and a manufacture method of the health-care medicine. The health-care medicine comprises the following ingredients at the formula ratio: 5-12g of broccoli extract, 3.5-10g of astragalus mongholicus, 5-15g of codonopsis pilosula, 5-15g of poria cocos, 4-12g of rhizoma atractylodis macrocephalae, 4-12g of oldenlandia diffusa, 3-9g of pseudo-ginseng, 0.007-0.015g of selenium carrageenan and 3.6-10g of microcrystalline cellulose. The preparation method comprises the following steps of: respectively smashing the astragalus mongholicus, the codonopsis pilosula, the poria cocos, the rhizoma atractylodis macrocephalae, the oldenlandia diffusa and the pseudo-ginseng; screening with a screen of 60-80 meshes; weighing and mixing various smashed raw materials and other raw materials and auxiliary materials at a certain ratio, wherein mixing time is controlled within 45-50 minutes; filling into a capsule; in the filling process, controlling the weight of a filler according to the inner quality standard; screening, polishing, internally packaging and externally packaging; and finally putting into storage after detection. The health-care medicine formula with functions of enhancing immunity, alleviating physical fatigue and resisting tumor can help people to enhance immunity, alleviate physical fatigue and resist tumor; the immunity of the human body is improved, the life quality of people is improved, and the health-care medicine formula does not have side effect or adverse reaction; and a modern preparation technology and an advanced quality control method are adopted, and therefore, the product is safe and stable and has controllable quality.

Description

The health medicine prescription of a kind of enhancing immunity, alleviating physical fatigue, anti-tumor function

Technical field:

The present invention relates to the health medicine prescription of a kind of enhancing immunity, alleviating physical fatigue, anti-tumor function, specifically is a kind of Caulis et Folium Brassicae capitatae stomach spirit sugar-tablet.

Background technology:

These article are suitable for the extensive crowd of hypoimmunity.Global the survey showed that according to one of World Health Organization (WHO), and the people of whole world true health only accounts for 5%, through doctor's inspection, diagnoses ill people also only to account for 20%, 75% people and then be in sub-health state.In May, 2002, the crowd that CPMA inference China is in sub-health state is 70% (about 800,000,000), and most people's hypoimmunity.

" in healthy energy deposited, heresy can not be done, and the institute of heresy gathers, and its gas must be empty ".Mean: when having very strong resistance in the human body, disease is impossible invade health, the disease place of arrival, and its resistance is weak certainly.The basic reason that the people falls ill is that resistance (medically claiming immunity) is low.Modern medicine study proves, eliminates subhealth state, prevent disease, and the most effectively way is exactly the immunity of enhances human body to disease.Therefore develop the effect uniqueness, have no side effect, the health product with function of enhancing immunity of high in technological content, pure natural, necessarily have vast market prospect and good social benefit.

According to market survey, though it is many to have the health product of identical function in the market, market demand is still huge, in addition with the Caulis et Folium Brassicae capitatae nutritious, that effect is unique be the primary raw material development product but seldom, so market remains exploitation.These article have following characteristics: this formula for a product rationally, definite ingredients, effect be reliable; Take safe, without any side effects; Easy to carry, be convenient to preserve; Process technology is advanced, quality guaranteed (these article production and processing overall process is carried out in strict accordance with the GMP standard).Caulis et Folium Brassicae capitatae in the prescription is the sharpest edges of these article; The contained nutrient substance of Caulis et Folium Brassicae capitatae is very abundant; Not only contain protein, sugar, fat, mineral, vitamin C and carotene etc., glucosinolate content is very abundant especially, is main with Radix Raphani sulfur glycosides (4-methylsulfinyl cyclobutenyl thioglycoside); Its catabolite sulforaphen is the strongest active component of finding in the vegetable up to now of anticancer vigor; Have the obvious microenvironment of improving human body cell, can improve body immunity, promote the effect of death of neoplastic cells.Therefore than other products, the health-care effect of this product is even better.

China is engaged in the research and the production of natural drug; Advantageous condition is arranged; The rarity of Traditional Chinese Medicine, it has clinical experience in several thousand, from present pharmaceutical research trend; From natural product, seek new drug or find that the guide is combined to thing and it is carried out the important content that structural modification is a drug development, along with the People more and more that day by day seriously makes of social environmental pollution is liked pure natural, pollution-free, the food that do not add synthetics.Adding growth in the living standard also makes people that the quality of life has been had higher pursuit.Under this background, this prescription utilization high-tech means and the hypoglycemia healthcare food that is raw material with free of contamination pure natural animals and plants arise at the historic moment, and develop rapidly.

Summary of the invention:

The health medicine prescription that the purpose of this invention is to provide a kind of enhancing immunity, alleviating physical fatigue, anti-tumor function; Its spy, it can strengthen people's immunity, alleviating physical fatigue, assist people antitumor, improves people's living standard; Have no side effect and untoward reaction; And use modern preparation technique, advanced method of quality control makes product accomplish safety, stable, quality controllable.

In order to solve the existing problem of background technology, the present invention adopts following technical scheme: its prescription ratio is: Caulis et Folium Brassicae capitatae 5-12g, Radix Astragali 3.5-10g, Radix Codonopsis 5-15g, Poria 5-15g, Rhizoma Atractylodis Macrocephalae 4-12g, Herba Hedyotidis Diffusae 4-12g, Radix Notoginseng 3-9g, selenocarrageenan 0.007-0.015g, microcrystalline Cellulose 3.6-10g;

Its preparation technology is: earlier said medicine is pulverized respectively; Cross the 60-80 mesh sieve, the various material powders and other supplementary material that weigh according to the above ratio after the pulverizing mix, incorporation time control 45-50 minute; Carrying out capsule then fills; Filling process is according to inner quality standard control implant weight, screens, polishing, inner packing, outer package, check warehouse-in at last again;

Content active constituent content after above-mentioned processing technique distributes as follows in the said medicine: composition: the 100g medicine contains the sweet 375mg of effective composition Radix Raphani sulfur, naphthoquinone 2400mg, crude polysaccharides 8000mg, total saponins 13000mg.

The present invention has following beneficial effect: can enhancing immunity, alleviating physical fatigue, anti-tumor function; Improve people's health level, have no side effect and untoward reaction, and use modern preparation technique; Advanced method of quality control makes product accomplish safety, stable, quality controllable.

The specific embodiment:

This specific embodiment adopts following technical scheme: its prescription ratio is: Caulis et Folium Brassicae capitatae 5-12g, Radix Astragali 3.5-10g, Radix Codonopsis 5-15g, Poria 5-15g, Rhizoma Atractylodis Macrocephalae 4-12g, Herba Hedyotidis Diffusae 4-12g, Radix Notoginseng 3-9g, selenocarrageenan 0.007-0.015g, microcrystalline Cellulose 3.6-10g; Its preparation technology is: earlier the Radix Astragali, Radix Codonopsis, Poria, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, Radix Notoginseng medicine are pulverized respectively; Cross the 60-80 mesh sieve, the various material powders and other supplementary material that weigh by a certain percentage after the pulverizing mix, incorporation time control 45-50 minute; Carrying out capsule then fills; Filling process is according to inner quality standard control implant weight, screens, polishing, inner packing, outer package, check warehouse-in at last again.

The present invention has following beneficial effect: can strengthen the people can enhancing immunity, alleviating physical fatigue, anti-tumor function; Improve people's health level; Have no side effect and untoward reaction; And use modern preparation technique, advanced method of quality control makes product accomplish safety, stable, quality controllable.

Caulis et Folium Brassicae capitatae:

Caulis et Folium Brassicae capitatae raw material in the prescription is processed through alcohol extraction process with our common vegetable Caulis et Folium Brassicae capitatae used in everyday.Caulis et Folium Brassicae capitatae meets " Ministry of Public Health is about the notice of further standard healthy food material management " requirement as the bread and cheese raw material; Caulis et Folium Brassicae capitatae is with its nutrient substance that is rich in and the unique effects that has; Along with progressively going deep into of scientific research; More and more receive people's attention, and beginning has obtained exploitation faster and has used at field of food.Caulis et Folium Brassicae capitatae not only contains protein, sugar, fat, mineral, vitamin C and carotene etc.; Glucosinolate content is very abundant especially; With Radix Raphani sulfur glycosides (4-methylsulfinyl cyclobutenyl thioglycoside) is main, and its catabolite sulforaphen is the strongest active component of finding in the vegetable up to now of anticancer vigor, has the obvious microenvironment of improving human body cell; Can improve body immunity, promote the effect of death of neoplastic cells.

External relevant bibliographical information Caulis et Folium Brassicae capitatae have function of enhancing immunity; P.Thejass for example; Experimental result shows in " probe into the immunocompetence of sulforaphen, a kind of from Brassica oleracea L. var. botrytis L. the isothiocyanate of natural extract " literary composition that G.Kuttan delivers on " plant amedica (magazine) ": sulforaphen has the effect of the peritoneal macrophage that improves activate the phagocytic capacity.The effect of sulforaphen also can significantly reduce the tumor necrosis factor that is increasing in addition--and-a lipopolysaccharide stimulates the macrophage that produces.These results show that sulforaphen has the immunocompetent effect of adjusting.(seeing the 14.3-1 of declaration material for details); According to Thejass P, Kuttan G is in that " " isothiocyanate that sulforaphen is a kind of to derive from the nature of Caulis et Folium Brassicae capitatae increases the cytotoxicity that BALB/c mouse NK cell and antibody rely on through strengthening interleukin II and tumor necrosis factor " literary composition of delivering on immune pharmacology and the immune toxicity journal is reported: sulforaphen is to normally and the research of the influence of the cell-mediated immunity of the BALB/c mouse of Ehrlich ascites inoculated tumour.Sulforaphen significantly increases the NK cytoactive of normal and inoculated tumour animal.NK cytoactive among the intact animal is observed than inoculated tumour cell animal is more Zao.The cytotoxicity that the antibody of the animal of the normal and inoculated tumour that sulforaphen is handled relies on is significantly strengthened than the animal of the inoculated tumour that does not have sulforaphen to handle.In the animal of the normal and inoculated tumour that sulforaphen is handled, observe a kind of cytotoxicity of complementation mediation of early antibody dependence.Sulforaphen is handled interleukin II and tumor necrosis factor-gamma in the animal that has significantly increased normal and inoculated tumour.Sulforaphen is handled and has significantly been promoted splenocyte, medullary cell, thymocyte proliferation in addition.Showed that sulforaphane with enhanced immune function; According Thejass? P, Kuttan? G in the "immune pharmacological and immunotoxicity management magazine" published "sulforaphane on B16F-10 melanocytes tumor induced migration tumor inoculation C57BL / 6 mice cell-mediated immune response regulation, "a paper reported: sulforaphane treatment significantly enhanced Migration tumor inoculation animals NK cell activity, a significant increase in melanoma vaccination animals interleukin-2 and tumor necrosis factor α, another sulforaphane significantly reduced in tumor metastasis during serum proinflammatory cytokine toxicity level through sulforaphane stimulate cell-mediated immune response, increased IL-2 and IFN-gamma, reduced proinflammatory toxicity factor IL-1beta, IL-6, TNF-alpha and GM-CSF.

Domestic relevant bibliographical information Caulis et Folium Brassicae capitatae have function of enhancing immunity; Show in " sulforaphen extraction, separation and Anticancer Activities in the Caulis et Folium Brassicae capitatae " literary composition that for example Ji Yubin, Chi Wenjie, Zou Xiang, Wang Zhiju deliver that sulforaphen has tangible blocking effect especially obvious to hepatocarcinoma, the esophageal carcinoma, preceding gastric cancer effect to rodent hepatocarcinoma, breast carcinoma, pulmonary carcinoma, the esophageal carcinoma, preceding gastric cancer on " Harbin University of Commerce's journal " (changing the science version over to); Experiment proof they can Phase II (cancerigenic factor detoxifcation) enzyme generation; Thereby play antitumaous effect; These enzymes can be through the active center of destroying cancerigenic factor or the method that they and endogenous aglucon are combined, and acceleration excretes it.Data proves that sulforaphen is through carcinogen metabolic regulation mechanism is worked.The research worker of vast this John Hopkins University of the U.S. is utilized transgenic mouse experiment proof: a kind of system of ubiquity in many animal bodies, the carcinogenesis that is used for resisting toxic chemical substance in food and the environment.The performance of this effect depends on the remarkable increase of Phase II enzyme quantity.This enzyme can be eliminated harmful chemical, suppresses its infringement DNA, thereby reaches anticancer disease effect.Two sulforaphen are the most intensive Phase II enzyme inducers of finding up to now; Deliver in " purification and the anti-tumor activity of main sulfur glycosides in the Semen Brassicae campestris " literary composition in " applied chemistry " (the 22nd the 10th phase of volume) according to the Zhou Jinlan of department of chemistry of the Central China University of Science and Technology, Yu Kaichao and to show: the sulfur glycosides itself is kind of a more stable chemical compound, but can be become multiple products such as isothiocyanate (ITC), oxazolidine thione (OZT) and nitrile compounds by bacterial enzyme catalytic degradation in myrosase or the gastrointestinal tract.The antibacterial action that these catabolites have stronger inhibition fungus and microbial reproduction etc. reaches through inducing the carcinogenesis of the active blocking-up of ubiquinone reductase carcinogen; According to first middle school, Qinhuangdao, Hebei province, Hebei Normal University chemistry and material science institute Shen tree virtue, Zhang Yingfeng, nightstool river " chemistry teaching " (2009; The 7th phase) shows in " the anticancer pharmacology of thioglycoside in the Caulis et Folium Brassicae capitatae " literary composition of delivering on that Radix Raphani sulfur glycosides can induce body to produce the II type and separate toxenzyme-glutathione transferring enzyme and quinone reductase; This enzyme can produce resistance to many carcinogens, thereby plays antitumaous effect; According to the Beijing University of Technology engineering constraint learn, that The Hong Kong Polytechnic University's applying biological and chemical science and technology is Wang Jiandong, Yuan Qipeng, Qian Zhongming points out that sulforaphen acts on the body process in " sulforaphen progress " literary composition that " food and fermentation industries " delivered is following: (1) enzyme II gene transcription.Transcribe the II type enzyme that only produces the human body beneficial, inhibitory enzyme I produces simultaneously, and enzyme I can produce cooperative effect with carcinogen, quickens cancer and takes place.(2) enzyme II acts on the anticancer system of body self.Through this system, can realize following regulation and control: 1. block carcinogen and produce the required metabolic pathway of carcinogenic effect, stop carcinogen or its metabolite to get into target cell, play the carcinogenic effect of removing simultaneously to metabolism; The division of anticancer and growth, blocking-up circulation approach; But the red carcinogen of the protein secreting ancestral who promotes other kill cancer cell is induced the effect of cancer.2. the hydrolyzate of the sinigrin startup generation effect that possibly play a role to carcinogen; At the cancer formation initial stage gene is undergone mutation; Also have some inhibitory enzymes to have dual resistance cancer or cancer suppressing function in addition, Toxic is degraded, thereby play anticancer effect-become and press down the cancer approach; According to Zhejiang Prov Industrial And Commercial University's food is that Wang Zhongying, Wang Xiangyang show in " thioglycoside and catabolite isothiocyanate thereof " literary composition that " chemistry of life " (rolling up for 4 phases in 2005 25) delivered: the anticancer mechanism of isothiocyanate comprises two parts: (1) is through suppressing the process that the Phase I enzyme stops (comprising cytochrome p450 system) chemocarcinogenesis; Particularly PEITC and BITC can effectively suppress cancerigenic factor-DNA and add the formation with thing; Thereby suppress forming by the inductive tumor of cancerigenic factor; (2) induce the generation Phase to separate toxenzyme; For example glutathione transferase and UDP-glucuronyl transferase etc., thus the overactive carcinogenic intermediate product that cytochrome p450 system produces reduced.Wherein SUL induces and produces Phase to separate the ability of toxenzyme strong especially, is the natural inducer that the most effectively Phase that identifies is up to now separated toxenzyme.Though human body is had certain toxic action for the SUL of high concentration and fragrant alkane isothiocyanate but they (in 0.05～20umol), do not show 200～400 milligrams of the oral suggestions of toxicity human body, can effectively prevent multiple cancer in valid density.

With the Caulis et Folium Brassicae capitatae consumption in this prescription, according to hide naval, Zhang Keying shows in " harm of thioglycoside and detoxification Study on Measures in the dregs of rapeseed cake " literary composition that " feed industry " (2007 the 28th volumes) delivered: pig, rabbit, fowl, fish and ruminant to daily ration in the tolerance level of thioglycoside be respectively 0.78,7.0,5.4,3.6 and 1.5～4.22umol/g; Show in " applied chemistry " (the 20th the 10th phase of volume) " purification and the anti-tumor activity of main sulfur glycosides in the Semen Brassicae campestris " literary composition,, mice sulfur glycosides least action dosage 30mg/kg shows that the sulfur glycosides has obvious inhibitory action to S180 tumor in the mice body; Show in " thioglycoside and catabolite isothiocyanate thereof " literary composition that deliver " chemistry of life " (2005 25 volume 4 phases): though the SUL of high concentration and fragrant alkane isothiocyanate to human body have certain toxic action they in valid density (in 0.05～20umol); Do not show 200～400 milligrams of the oral suggestions of toxicity human body, can effectively prevent multiple cancer.According to above different documents and materials, be test raw material with Radix Raphani sulfur glycosides in this prescription Caulis et Folium Brassicae capitatae raw material, experimentize by adult (50kg weighing machine) 34mg every day.

1. 30 times, 10 times of human body recommended amounts are established in ear swelling method experiment, calculate according to formula: promptly every day, Radix Raphani sulfur glycosides 22.48mg/kg, 8.16mg/kg were respectively high low dose group.Actual disposition needs the high low dose group 27.98mg/kg of sample Radix Raphani sulfur glycosides, 9.33mg/kg, dosage 0.4ml/20g Mus weight/d when irritating gastric juice.Every day gastric infusion, experimental result is seen table 1, table 2.

Table 1 Radix Raphani sulfur glycosides immunological enhancement: body weight organ index

Table 2 sulforaphen Radix Raphani sulfur glycosides immunological enhancement: ear swelling

Experimental result compare the high low dosage of Radix Raphani sulfur glycosides with blank control group to not obviously influence of body weight, but the ear swelling experiment shows that high low dose group all has tangible immunological enhancement.

2. humoral immunization serum hemolysin experiment, the high 1.2mg/ml of Radix Raphani sulfur glycosides; Radix Raphani sulfur glycosides hangs down 0.4mg/ml, dosage 0.4ml/20g/d, every day gastric infusion.The result sees table 3 table 4:

Table 3 sulforaphen Radix Raphani sulfur glycosides immunological enhancement: body weight organ index

Table 4 sulforaphen Radix Raphani sulfur glycosides immunological enhancement: serum hemolysin

Experimental result is compared with blank control group, and the positive drug Thymosin alpha 1 is influential to body weight, and the determination experiment of serum hemolysin shows its obviously enhancing body humoral immunization.All the other each groups compare with normal group, and body weight and organ index are not all had remarkable effect.The serum hemolysin and the antibody product of the high low dosage of Radix Raphani sulfur glycosides have raising, p＜0.05, and low metering group also improves with respect to blank control group.The determination experiment of serum hemolysin shows that just metering group of Radix Raphani sulfur glycosides has certain enhancing immunity effect.

The Radix Astragali

Radix Astragali head is stated from Shennong's Herbal, sweet in the mouth, and slightly warm in nature is returned the spleen lung meridian, has the effect that same table, diuresis, promoting pus discharge and tissue regeneration strengthening are defended in spleen invigorating invigorating middle warmer, ascending up spleen-Qi and Yang, benefit.Modern study shows that the Radix Astragali contains materials such as multiple glycoside, polysaccharide, flavone, folic acid and various trace elements, has antitumor, regulates pharmacological actions such as immunity, antiviral, defying age, antioxidation, radioprotective and anti-stress.

1, the ¨ such as antitumor action 1 of the Radix Astragali discover, compare Ramulus et folium taxi cuspidatae with independent use paclitaxel treatment

1.1 human body immunity improving function stone Ren Bing etc. find in to the research of chemotherapy tumor-bearing mice immune function influence and tumor-inhibiting action at Radix Astragali S4; List all is higher than list with cyclophosphamide group and negative control group with thymus index, the index and spleen index that the Radix Astragali group and the Radix Astragali add cyclophosphamide group tumor-bearing mice, and difference has statistical significance; The proof Radix Astragali has protective effect to immune organ, and chemotherapy damage immunologic function is had better protect and promotes restitution.Tumor-bearing mice after the cyclophosphamide chemotherapy of the yellow late-maturing treatment of application such as Ying Zizhong immunocompromised; Find that IgM, IgG significantly increase in the tumor-bearing mice body; Explain that the Radix Astragali can significantly promote the thymus humoral response in anti-tumor immunotherapy, improve the specific immune response of host cancerous cell through stimulating the huge system of biting of monokaryon.Li Peiwen etc. find that also Radix Astragali injection can improve the tumor patient immunologic function, strengthens its humoral immunization and cellular immune function.

1.2 inhibition tumor cell proliferation; Research astragalus root components such as promotion apoptosis of tumor cells Zhao Lian China are in the inhibitory action of novel formulation to people CCL-229, K562 cancerous cell; Find that astragalus root components F3 novel formulation has the obvious suppression effect to 2 kinds of cancerous cell; And be the concentration dependence, more remarkable with cisplatin combined medication effect; The shrinkage of cancerous cell peplos, kytoplasm after the visible Radix Astragali effect of transmission electron microscope observing assembled, chromatin concentrates, and visible karyorrhexis and apoptotic body form.Liu Hui etc. " to discover; after the Radix Astragali acted on the transplanted tumor of lotus people squamous cell carcinoma strain GNM tumor bearing nude mice, mirror was observed down and is seen that the cancerous cell form is irregular, cavity is arranged in the endochylema; the part cell has the karyorrhexis phenomenon; and see the liquefaction and necrosis district, explain that the Radix Astragali has anticancer propagation, promotes its effect of apoptosis.

1.3 the influence heavy to tumor

The result shows that the tumor weight average of the test group of various dose is lower than negative control group (P＜0.05), and LL increases along with Radix Astragali dosage, and tumour inhibiting rate has the trend that increases gradually, and the tumor killing effect tool that shows the Radix Astragali is from. dose response fire system.

The influence that table 5 Radix Astragali is heavy to mice S180 tumor

Annotate: * representes to compare P＜0.05 with negative control group

1.4 to immune influence

The result shows that the T lymphproliferation response of positive controls and NK cytotoxic activity all are lower than negative control group (P＜0.05), shows that cyclophosphamide has certain damage to immune system; Use the T lymphproliferation response and the NK cytotoxic activity of three experiment knobs of the Radix Astragali all to be higher than negative control group (P＜0.05); And increase along with dosage; Above-mentioned two indexes has the trend that increases gradually, shows that the Radix Astragali can strengthen immune function of mice, and dose-response relationship is arranged.

Table 6 Radix Astragali is to immune influence

Annotate: compare P＜0.05 between two dose groups in twos; Compare * P＜0.05 with negative control group

The Radix Astragali is a kind of effective biological regulator, and the research of rice in recent years shows that the Radix Astragali has great potential at the control towel of tumor.This research is successfully being duplicated on the mouse-borne tumor model based; Adopt the astragalus polysaccharides of various dose to intervene; The tumor weight average was lower than negative control group (P＜0.05) when the mice that the result shows all experimental grouies was finished in experiment, and along with the increase of dosage, tumor heavily has the trend (P＜0.05) that reduces gradually; Show that astragalus polysaccharides has tumor-inhibiting action, and there is dose-effect relationship in this effect.Under normal circumstances, be in poised state between the defence of tumor and body, the generation of tumor and even propagation and send out are due to the imbalance of this dynamic equilibrium fully.If will be imbalance state be adjusted to normal level artificially, may command tumor growth even it is disappeared then.Its host immune function of tumor patient is disequilibrium; The immunoreation of advanced tumor body almost disappears, and traditional chemotherapeutics especially selectively kills and wounds normal cell in the kill tumor cell; Particularly the hemopoietic tissue cell usually causes body generation severe complication.This result of study shows that the Radix Astragali can improve the lymphproliferation response that leans on the tumor mice, also can improve the NK cell activity, explains that the Radix Astragali suppresses tumor through human body immunity improving power.

Radix Codonopsis

Radix Codonopsis is a China tradition rare Chinese medicine, " Pharmacopoeia of People's Republic of China) 2005 editions Radix Codonopsis that record are the dry root of campanulaceae plant Radix Codonopsis, plain flower Radix Codonopsis or radix codonpsis tangshen, have invigorating the spleen and replenishing QI; Spleen invigorating cures mainly deficiency of the spleen and lung, the cardiopalmus of breathing hard, anorexia and loose stool; Dyspnea due to deficiency cough, interior-heat disease such as quench one's thirst.Discovery Radix Codonopsis water extracts such as Jia Taiyuan have tangible potentiation to the activate the phagocytic capacity of Mus J774 macrophage; And infer that this effect is the enhanced basis of immunity of organism. observations such as Koryo pine think that magnetic treatment Radix Codonopsis medicinal liquid has the effect that promotes the mononuclear phagocyte system phagocytic function, shows good action to immune system. Zhong Zhimin etc. detect Radix Codonopsis can promote PMNC CD ²⁺The growth of cell; The prompting Radix Codonopsis helps to improve discovery Radix Codonopsis such as immunologic function .B E Shan can stimulate lymphopoiesis; But act on faint; Observing Radix Codonopsis simultaneously Ig and IL-1 are had certain effect. Wang Min etc. find that in the mice aging model that causes with D one galactose Radix Codonopsis and compound recipe thereof can improve the IL-2 level of mice; Thereby raise immunity; Play function in delaying senility. observations such as Tang Qingxiu find that the Radix Codonopsis essence is produced in Gansu and Radix Codonopsis polysaccharide can improve the Turnover of Mouse Peritoneal Macrophages phagocytic function; And increase immune organ weight. Cao Li etc. and Li Yan etc. have inquired into the effect to normal spleen lymphocyte proliferation of Radix Codonopsis polysaccharide and codonopsis clematidea Clarke's polysaccharide respectively; All find to have facilitation; And the former acts on obviously. and Cao Li etc. find that also Radix Codonopsis polysaccharide has tangible promotion proliferation function equally to the lymphocyte that concanavalin A, Con A stimulates. and the antibody horizontal in the immunologic process also is an aspect of reaction immunoregulation effect. and experiments such as Yang Guang show that Radix Codonopsis polysaccharide generates normal mouse antibodies potentiation is arranged. Zhang Xiaojun etc. have reported that Radix Codonopsis polysaccharide has strong facilitation to humoral immunization, and the pair cell immunity has experiment discovery Radix Codonopsis polysaccharide such as facilitation .Z T Wang that immunosuppressive action is arranged when low dose of administration, but the pinocytosis process of macrophage is not had effect; It is active that discovery codonopsis clematidea Clarke polysaccharide such as Xiong Yuanjun can increase SOD in the body. and discovery Radix Codonopsis such as Huang Liya and injection thereof can raise the expression of (GSH-Px); The prompting Radix Codonopsis can be resisted oxygen free radical injury; Belonging to the strong anti-oxidation medicine also plays an important role in the defying age process. and Radix Codonopsis also has the effect of removing free radical. and the scavenging action to ultra-oxygen anion free radical of Radix Codonopsis polysaccharide and codonopsis clematidea Clarke's polysaccharide is measured and proved to the different reaction systems of employing such as Li Guirong etc. and Xiong Yuanjun respectively; The former also finds the scavenging action of Radix Codonopsis polysaccharide to hydroxyl radical free radical; The latter tests the generation that proof codonopsis clematidea Clarke polysaccharide can reduce MDA in vivo. and the anti-aging effects of Radix Codonopsis mainly shows in the following areas. and discovery rats such as Wang Shaoshen begin to give Radix Codonopsis to the geratic period from the postadolescence; Can delay the aging of hypothalamus one endocrine network through the degree of injury that alleviates the central nervous system; Improve the central nervous system function state indirectly; Thereby reaching the purpose of slow down aging. Wang Min etc. [71] find that plate party can improve senile organism IL one 2 levels, thereby repair the immunosenescence of aging body and play function in delaying senility. king's equality finds that plate party can improve the histologic lesion of subacute aging model mouse brain, thus slow down aging. Xu Aixia etc. [73] experiment proof Radix Codonopsis polysaccharide can the human body immunity improving function; Remove free radicals such as active oxygen in various degree; Reduce level of lipid peroxidation and raise NO in the body, improve the organism physiology function, the performance anti-aging effects.

1, Radix Codonopsis polysaccharide is to the abdominal cavity influence of lotus Sm ascites tumor cell mouse time-to-live.

1.1S ₁₈₀The cultivation of going down to posterity of ascites tumor cell: aseptic extraction inoculation back 5d well-grown abdominal cavity lotus S ₁₈₀Mouse ascites, and (oncocyte content is about 510 to become tumor cell suspension with physiological saline solution by 1: 3 dilution proportion ⁷/ m), every mouse peritoneal is penetrated tumor cell suspension 0.2mL, goes down to posterity 1 time to keep S in per 5 days ₁₈₀The vigor of ascites tumor cell.Can find out by table 7, to abdominal cavity S ₁₈₀The increase in life span that the ascites tumor cell mouse is irritated stomach 1g/ (kgd) Radix Codonopsis polysaccharide is 34.29%, compares with negative control group, and P＜0.05 shows that polysaccharide is to abdominal cavity lotus S ₁₈₀The ascites tumor cell mouse has certain life prolongation effect, has certain antitumor action.

1.2 anti-tumor experiment: inoculation S ₁₈₀Behind the ascites tumor cell 24, be divided into experiment drug group 3 dosage, negative control group and positive controls (Cy) at random, 10 every group.The experiment drug group press 0.4mL/10g gastric infusion every day 1 time, 7d continuously, branch 0.5g/ (kgd), 1g/ (kgd) and three dosage of 2g/ (kgd); The gastric infusion next day of positive controls cyclophosphamide 50mg/kg is given 3 times; Negative control group is irritated the stomach normal saline every day, continuously 7d.Continue to observe animal to dead, write down the existence natural law of tumor-bearing mice, calculate the increase in life span of tumor-bearing mice

Table 7 various dose group medicine is to inoculation S ₁₈₀The influence of ascites tumor mice time-to-live

Compare * P＜0.05 Poria with matched group

Poria is a conventional Chinese medicine, and history is stated from Shennong's Herbal, is classified as top grade.Dry sclerotia for the Polyporaceae fungus.The property slightly sweet flavor, flat, classify heart spleen lung meridian.Effect with damp eliminating diuretic, invigorating the spleen and regulating the stomach, mind tranquilizing and the heart calming is used to treat dysuria, the edema distension, and the phlegm retention cough with dyspnea, it is dirty to vomit, have loose bowels, seminal emission, stranguria with turbid discharge, palpitation with fear, forgetful etc.In the last few years, both at home and abroad composition such as pachyman, three mushrooms and pharmacological action thereof, biological activity aspect had been done more research, and bigger progress had been arranged.

1, pachyman is to test chicken lymhocyte transformation rate, NK cytoactive and the active influence of M φ

The lymhocyte transformation rate of each test group chickling different days, NK cell killing activity, M φ activity all are significantly higher than matched group; But lymhocyte transformation rate, NK cytoactive, M φ activity with middle dosage pachyman (2mg/ of every day) group are the highest, and the index of correlation that high dose (3mg/ of every day) group and low dosage (1mg/ of every day) are organized is lower.The different days testing result relatively; Lymhocyte transformation rate, NK cell killing activity, active 3 indexs of M φ are significantly higher than the testing result of 7,42,56 ages in days when 14,28 ages in days; And do not have significant difference between age group on 14th, 28, there is not significant difference between age groups on the 7th, 24,56.

Table 8 pachyman is to chickling lymhocyte transformation rate, active, the active influence of M φ of NK

2, to Immune Function

2.1 activate the immune surveillance system of body

Pachyman can obviously strengthen the breeder reaction that spleen T lymphocyte stimulates ConA; Can strengthen the anti-infective ability, activate and the enhancing bone-marrow-derived lymphocyte, can induce the early young grain leukocyte of people to be divided into mononuclear phagocyte; Can strengthen the macrophage recognition function; Improve the phagocytic index of macrophage and TNF is discharged and increase, strengthen especially cytotoxic T lymphocyte (CTL) activity of cellular immunization, bring into play the effect of killing tumor cell indirectly; Raise mice specific cellular immunity and humoral immune function, but non-stimulated mouse immune organ thymus and the outgrowth effect of spleen.Lee etc. have reported that pachyman makes before the transcription factor NF-KB/ReL activate and raise iNOS gene expression to realize through activating 38 kinases to the activation of macrophage.Pachyman can promote the Turnover of Mouse Peritoneal Macrophages metabolism, makes its quantity, form and cell membrane enzymatic activity that obvious change all take place, and it gulps down drink, phagocytic function and lysosomal enzyme content and discharge and all can significantly improve.

2.2 influence to complement system

Complement is the important component of congenital main body defence, and tens protein that complement system contains are present in normal human or animal's the serum.The mechanism of pachyman enhancing macrophage phagocytic function is relevant with activating complement, and it is through alternative route activating complement system, and this active mode does not rely on the formation of specific antibody, is a kind of effective, early immune defense mechanism in the body.

2.3 induce and urge to induce effect

Experimental studies such as Yang Ji becomes show; Carboxymethyl pachyman induces effect; Can induce PBLC to produce IL-2, TNF, IL-6, IFN-γ separately; And can work in coordination with PBLC or ConA and stimulate PBLC secretion IL-2, TNF, IL-6, IFN-γ to increase, be again a kind of strong short inducer of imitating.There is report to show that the inducer IL-2 of carboxymethyl pachyman is effective medicine of tumor.

2.4 removing oxygen-derived free radicals

Arachidonic acid is the height polyunsaturated fatty acid, and is easy in free radical attack in the cell, the generation peroxidization, and generation has toxicant malonaldehyde (MDA) carcinogenic and the promotion tumor growth, and pachyman can strengthen the liver SOD activity and the removing oxygen-derived free radicals.According to bibliographical information, the growing amount of MDA significantly increases in the cancerous tissue, reduces the arachidonic level that tumor tissues obviously raises, and reduces radical damage, also has certain antitumous effect.The Sulfation pachyman has regulating action to mouse immune organ active nitrogen level.

2.5 influence DNA, RNA and the effect of protein biosynthesis

It is synthetic that pachyman can suppress tumor cell DNA, and GO, GL phase cell are increased, and S, G2 phase cell descend.Report is arranged, and carboxymethyl pachyman (CMP) has direct cytotoxic effect to the mice ehrlich ascites cell, and DNA is synthetic to be realized its inhibitory action through suppressing, and inhibitory action increases with the increase of dosage.Pachyman also possibly influence the transcription of DNA, and the mRNA level is increased, and then makes it express the growing amount increase.

2.6 promotion apoptosis of tumor cells

The Sulfation pachyman can strengthen the expression of Bax gene, reduces the expression of apoptosis suppressor Bcl-2, promotes the apoptosis of S180 tumor cell.Carboxymethyl pachyman can be through the gene induced cancer cell-apoptosis of downward modulation bcl-2.Have the Synergistic anti-cancer effect after carboxymethyl pachyman CMP, selenium and cyclophosphamide share,, thereby prolong life cycle of tumor-bearing mice, alleviate the toxic and side effects of chemotherapeutics simultaneously through downward modulation bcl-2 expression of gene, cancer cell specific induction of apoptosis.The pachyman of Sulfation possibly start apoptosis of tumor cells and reach the antineoplastic purpose through raising the Fas positive expression.

The Rhizoma Atractylodis Macrocephalae

The Rhizoma Atractylodis Macrocephalae is a Chinese medicine, the beginning be stated from Shennong's Herbal, classify as top grade, original name " art ", feverfew, warm in nature for Rhizoma Atractylodis Macrocephalae stem, sweet in the mouth, hardship.. in recent years; Chinese scholars has been made a large amount of research work for the effect of aspects such as the antitumor of Rhizoma Atractylodis polysaccharide in human body, defying age, adjusting immunologic function, enhancing immunity, blood sugar lowering, and the Application Research of Rhizoma Atractylodis polysaccharide in animal produces also also has report.

1, the Rhizoma Atractylodis Macrocephalae is to the influence of immune organ

Peng Xinguo etc. (2001) discover that the Rhizoma Atractylodis Macrocephalae has tangible effect of gain to mouse spleen and thymus.Spleen is one of place that produces primed lymphocyte and antibody, and can eliminate materials such as senile cell and microorganism, and a large amount of T lymphocytes, bone-marrow-derived lymphocyte and macrophage wherein plays an important role in body defence and removing function.Thymus is important central immune organ, also is the sophisticated place of T cell development.Therefore, how extremely close with body's immunity and pathological changes relation the function of thymus and spleen is.According to Zhang Lecui (2002) etc., Chinese medicines such as Radix Codonopsis, the Radix Astragali, white stake are formed herbal mixture with 1%TMP and are added in the feedstuff, and the result can effectively prevent and remove the immunosuppressant due to the IBDV, and makes the organizational structure maintenance or the recovery normal level of immune organ.

2, the influence of Rhizoma Atractylodis Macrocephalae pair cell immunity

Lymphocyte comprises T lymphocyte and bone-marrow-derived lymphocyte, is induced, breaks up and grown by thymus, fabricius bursa and bone marrow respectively, mainly is present in blood and the peripheral immune organ.Transform after the primary stimuli of T lymphocyte receptor mitosis, weigh the immune state of lymphocytic function and state and body usually with the height of its conversion ratio.

3, the Rhizoma Atractylodis Macrocephalae is to the influence of humoral immunization

Humoral immunization is meant by the antigenic stimulus bone-marrow-derived lymphocyte, causes B cell activation, propagation and differentiation, forms plasma cell, and produces antibody, and bring into play the process of a series of immunological effects by antibody.The humoral immunization of animal body is by antibody-mediated, and antibody horizontal just is one of important indicator of its immune state of assessment in the serum.Cai Yumei (2001) etc. form golden pheasant with 20 flavor Chinese medicines such as Rhizoma Coptidis, the Radix Astragali, Radix Scutellariae, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Isatidis and loose; Immunity adopted 2.5% golden pheasant to loose the same day, and immunity has obvious synergistic effect to spice logotype 5d to newcastle; Can significantly improve the content of ND antibody titer and IgG in the serum, the IgG peak persistent period is prolonged, and delay the decline of IgA, IgM; Its immunopotentiation is superior to levamisole, improves the humoral immune function of body.This country flat (2002) adds 2% herbal mixture (Radix Astragali, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, Radix Glycyrrhizae, Herba Portulacae, Massa Medicata Fermentata, Fructus Crataegi and Radix Angelicae Sinensis) in pig starter feed.Behind the 14d, the serum IgG level improves 111.5% (P＜0.01), and the C4 level improves 48.6%, and the C3 level improves 97.8% (P＜0.01).(20 study carefully discovery Rhizoma Atractylodis Macrocephalae antagonist formation cell has stimulation to Peng Xinguo, and the antibody-secreting amount is increased, and Guo Fengli etc. (2003) also draw identical conclusion.Song Haiying etc. (2004) use 100% Rhizoma Atractylodis Macrocephalae leachate regularly to give mouse stomach, find that test group can obviously promote mice serum IgG content to increase, and improve 37.59% than matched group, and difference is (P＜0.01) extremely significantly.

4, the regulation and control of the Rhizoma Atractylodis Macrocephalae pair cell factor

Cytokine is the center adjustment factor of body immune system, by the secretion of various kinds of cell such as lymphocyte, NK cell and macrophage, the immunity of adjusting is arranged, and participates in multiple function (Dinarello, 1986 such as inflammatory reaction; Miossec, 1986; Beutler etc., 1987).Be divided into following several big type according to its structure and function: white thin be situated between plain (IL), interferon (IFN), colony stimulating factor tumor necrosis factor (TNF), transforming growth factor (TGF) and voltinism cytokine man bunch (Jin Baiquan, 2001).Cytokine has one type of key protein material of immunoregulation effect.Many medicines can pass through activated mononuclear. and macrophage system induces multiple intracellular cytokine (generate as promoting interferon, promote interleukin to generate, give birth to tumor necrosis factor etc.) and comes the enhance immune function.Interleukin (IL) ability activating B cell, macrophage, NK born of the same parents discover that in recent years many Chinese medicines have the effect that promotes that cytokine produces.According to Wang Longmei (2000), the Rhizoma Atractylodis Macrocephalae can obviously improve the human peripheral lymphocyte of ConA thorn war proclamation setting forth the purpose of the expedition and enumerating the crimes of the enemy and express the IL-2 receptor.Rhizoma Atractylodis polysaccharide PAM can improve T lymphocytic emiocytosis IL-2 can be used for oncotherapy the time, promote the generation of endogenous IL-2, play the auxiliary treatment effect.Hu Tao (2004) finds that the content of IL-1, IL 1 in the serum obviously raises after chemotherapy injured mice is irritated stomach 10d with Rhizoma Atractylodis Macrocephalae solvent.Wang Peixun (1998) report, compound recipe kidney qi soup can significantly improve suffers from a deficiency of the kidney and mice with spleen deficiency IL-1 activity, and it is active that decoction of four noble drugs can improve mice with spleen deficiency IL-1, and invalid to the mice of suffering from a deficiency of the kidney, two compound recipes all do not have influence to normal mouse IL-1 activity.

5, the Rhizoma Atractylodis Macrocephalae is to the influence of the lame weight of mice S180 meat

Compare with model group, the tumor weight of each dose groups of the Rhizoma Atractylodis Macrocephalae and cyclophosphamide group significantly reduces (P is all＜0.05), but there was no significant difference (P＞0.05) between each dose groups of the Rhizoma Atractylodis Macrocephalae; Tumor weight also variant (P＜0.05) between each group of the cyclophosphamide group and the Rhizoma Atractylodis Macrocephalae.Cyclophosphamide group inhibitory rate to 65.42%, each dose groups of the Rhizoma Atractylodis Macrocephalae reaches 40%～50%, and with the certain gap of cyclophosphamide, but tumour inhibiting rate does not have notable difference between each group of the Rhizoma Atractylodis Macrocephalae.

Table 9 Rhizoma Atractylodis Macrocephalae is to the influence of mice S180 sarcoma weight

modelgroup*P＜0.05；Baizhu?group?△P＜0.05

6, to the influence of tumor-bearing mice tumor weight, thymus index, spleen index

The influence of table 10 pair tumor-bearing mice tumor weight, thymus index, spleen index

Compare * P＜0.05, * * P＜0.01 with model group

7, to the influence of tumor-bearing mice Plasma TNF-a, IL-2 content

The influence of table 11 couple tumor-bearing mice Plasma TNF-a, IL-2 content

Compare * P＜0.05, * * P＜0.01 with model group

The Rhizoma Atractylodis Macrocephalae is suppressed tumor growth in this experiment and amynologic mechanism is inquired into.Through observing the inhibitory action of variable concentrations Rhizoma Atractylodis Macrocephalae water extract to S180 sarcoma tumor-bearing mice tumor piece; Show that each dose groups of the Rhizoma Atractylodis Macrocephalae all can suppress tumor growth; With middle dose groups best results, in the Rhizoma Atractylodis Macrocephalae, small dose group can also improve S180 tumor-bearing mice thymus index.Thymus is important immune organ in the body, is T lymphocyte fully-developed place, and thymus index increases the explanation Rhizoma Atractylodis Macrocephalae can promote the thymocyte proliferation differentiation, promotes cellular immune function.In this experiment; Each dose groups medicine of the Rhizoma Atractylodis Macrocephalae all can improve the tumor-bearing mice thymus index; Wherein in the Rhizoma Atractylodis Macrocephalae, the small dose group effect is remarkable; Explain that Rhizoma Atractylodis Macrocephalae water extract can improve tumor-bearing mice immune organ situation, the function of immune system that damages because of the lotus tumor is had better protect and promotes restitution, and demonstrate certain dose-effect relationship.TNF-a and IL 1 are important indicators of reflection body's immunity, in oncotherapy, are bringing into play important effect.TNF-a is a kind of mainly by the activatory monokaryon one huge excretory soluble protein cytokine of system of biting, and has various biological functions such as the immunne response of adjusting, promotion cell Growth and Differentiation, with the body inflammatory immunoreation substantial connection is arranged.Concentration is lower in the normal condition lower body.An amount of TNF-a is essential to the protective reaction of body, but excessive TNF-a then produces injury response to body.Therefore, the biological agent of TNF-a and intravital content are closely related.The TNF-a content of many clinical report tumor patients is not to descend but rising; Thereby cause body cell factor regulating system disorderly; Excessive TNF-a can also produce pathology damage property reactions such as fat acid decomposition, vascular permeability increase, endogenous pyrogenicity, systemic bleeding necrosis simultaneously, and this also is that one of pathophysiological mechanism of the extensive depletion of whole body late period appears in tumor patient clinically.IL 1 is mainly produced by activated T h1 cell; It is the most important cytokine of host's antitumor; In the adjusting of cytokine network, play a crucial role, one 2 pairs of tumor cells of IL do not have direct cytotoxicity, through regulating the immune system performance GVT of body.Our research shows that each dose groups of the Rhizoma Atractylodis Macrocephalae all can reduce tumor-bearing mice Plasma TNF-a content, and wherein the dose groups effect is remarkable in art.The Rhizoma Atractylodis Macrocephalae can reduce tumor-bearing mice serum TNF _ a content; Possibly be because tumor is suppressed; Body's immunity is protected, and infection chance reduces relevant, also possibly be because oncocyte activates monocytic ability drop in the blood circulation in transfer process; Tumor-infiltrated cell is weakened by the tumor antigen stimulation simultaneously, causes TNF-a content to reduce.The big or middle dose groups of the Rhizoma Atractylodis Macrocephalae can improve mice plasma IL one 2 content, and this possibly be to promote mice Th1 cell activation by the Rhizoma Atractylodis Macrocephalae, produces IL 1, and then promotes T and B cell proliferation differentiation, enhanced NK cell, mononuclear cell killing activity, thus suppress tumor proliferation.The Rhizoma Atractylodis Macrocephalae demonstrates certain dose-effect relationship to TNF one a and the excretory regulating action of IL-2.Our result of study shows that the Chinese medicine Rhizoma Atractylodis Macrocephalae has the effect that suppresses tumor growth.

Herba Hedyotidis Diffusae

Herba Hedyotidis Diffusae is a Rubiaceae annual herb plant, and Herba Hedyotidis Diffusae is common southern medical herbs, and fecund grows in moist limit, field, roadside, limes marginis and meadow on the south the Changjiang river.Its cold in nature, sweet-bitter flavor, GUIXIN lung liver stomach large intestine channel.Have heat-clearing and toxic substances removing, dampness removing is treating stranguria, the eliminating carbuncle mass-dissipating efficacy.Often cure mainly carbuncle sore, laryngopharynx swelling and pain, venom and pyretic stranguria etc.Modern pharmacology shows that this medical instrument has antibiotic, antiinflammatory, antitumor, raise immunity. the polysaccharide in the Herba Hedyotidis Diffusae has the active effect of enhance immunity.Polysaccharide in the real 2 enhance immunity effect Herba Hedyotidis Diffusaes has the active effect of enhance immunity.After experimental appendicitis rabbit was used Herba Hedyotidis Diffusae extract, stable after rabbit body temperature descends, leukocyte obviously descended, and inflammation disappears basically, and remarkable therapeutical effect is arranged.Wu Shi etc. separate from the Herba Hedyotidis Diffusae water soluble ingredient and obtain having immunocompetent polysaccharide, utilize gas chromatogram and gas chromatogram one mass spectral analysis to confirm polysaccharide structures, show and contain glucose, galactose, xylose and arabinose.They have significant enhance immunity activity test shows, and sheep red blood cell Flos Rosae Rugosae piebaldism combination rate and lymphoblast conversion ratio can raise.Glycoprotein material in the Herba Hedyotidis Diffusae can significantly strengthen the proliferation activity of mouse boosting cell, and resists the generation of growth of tumor and other diseases through the immune system that stimulates body.Meng Shi etc. have carried out testing in the body to white mouse, the result show in this plant polysaccharide to S one 180 sarcoma cell suppression ratio up to 40%.The total flavones of Herba Hedyotidis Diffusae has the effect of enhancing body specific immune function and non-specific immunity.Experimental results such as Wang Shi show the Herba Hedyotidis Diffusae total flavones (15,30,60mgkg ^-1) promote of the breeder reaction of immunologic hypofunction mice by ConA or the inductive splenocyte of LPS, and improve the content of mice serum IL 1 and 1FN one γ; Herba Hedyotidis Diffusae total flavones (60mgkg ^-1) promote immunologic hypofunction mouse spleen 1gM antibody to form, and the murine interleukin due to the rising antitumor drug (cyclophosphamide or cytosine arabinoside) reduces.Old experiment such as Xu Shi shows; These article have remarkable facilitation to the tumor-bearing mice immune organ function, and the peritoneal macrophage phagocytic function is significantly strengthened, and hemolysis plaque and hemolysin level significantly improve; And can significantly promote Con, LPS to induce down T, bone-marrow-derived lymphocyte breeder reaction process; Can reduce the mice ear degree, the cellular immunization and the humoral immune function of tumor-bearing mice all had potentiation, have good cancer resistance; Herba Hedyotidis Diffusae has very strong facilitation to the proliferation activity of mouse boosting cell; It is active to the specific killing of tumor cell to strengthen mice and people's killer cell, and the generation and the monocytic cytokine that strengthen B cell antibody produce, and strengthens the phagocytic function of mononuclear cell to tumor cell.Stigmasterol in the Herba Hedyotidis Diffusae, B one sitosterol, oleanolic acid, ursolic acid, para-position coumaric acid etc. can stimulate the reticuloendothelial system hypertrophy, strengthen cytophagous ability four.Yuan Shi etc. carry out the emission spectrographic determination experiment and obtain: the content of canceration suppression ratio and its Se, Mn is proportionate, and does not have dependency with Zn, MO content.In addition, the Ti element can stimulate phagocyte, and body immunity is strengthened.These article have higher phagocytic activity, and the Ti element has played certain effect MI.Polysaccharide is to No. 1 ascitic type of lymphosarcoma and ehrlich carcinoma hypodermic type; Coumarins is to cervical cancer 14 types, sarcoma 180, liver substance type, and triterpenes all has inhibitory action [71 to No. 1 ascitic type of lymphosarcoma, cervical cancer 14 types, sarcoma 180, liver substance type.From the ethanol extraction of Herba Hedyotidis Diffusae, separate obtaining triterpenes, Coumarins, polysaccharide and sterols, the gained composition is carried out the tumor animal experiment study.I terpenoid antitumous effect is optimum as a result.Ursolic acid, oleanolic acid suppress sarcoma 180 strain growth, are antineoplastic effective compositions.The Tan Shi report obtains a fat-soluble effective site from the Herba Hedyotidis Diffusae ethanol extraction, main component is sterol and ursolic acid, and there is the obvious suppression effect at this position to kinds of tumor cells.Water miscible polysaccharide of itq experimental verification Herba Hedyotidis Diffusae such as Zhao Shi and lipophilic extract have significant inhibitory effect to the S-180 tumor cell, and have the protective effect to the chemical damage of chemotherapeutics.Zhang Shi etc.. I applying gene chip technology research Herba Hedyotidis Diffusae stigmasterol (SHD) suppresses the target gene regulation and control of human liver cancer cell S one 7721 growths.The result shows that SHD has the remarkable vitro inhibitory action to human liver cancer cell SMMC 1; SHD suppresses the effect of SMMC one 7721 cells and is worked in coordination with by many target genes, and coordinates to accomplish through the inside and outside signal transduction pathway of born of the same parents.Zhang Shi etc. 112 discover that Herba Hedyotidis Diffusae total flavones composition can return to normal expression with the gene of 15 remarkable high expresseds and 5 significantly low genes of expressing, the generation of these genes and hepatocarcinoma, develop closely related.Yu Shi etc. study demonstration, and Herba Hedyotidis Diffusae extract has the obvious suppression effect to the growth of human hepatoma cell strain Bel one 7402 cells, and is dose-dependence, and half-inhibition concentration (IC50) is 1.6g/L; Among the Bel 1 behind the drug effect smaller volume of oncocyte, karyokinesis picture significantly reduce, no obvious apoptosis phenomenon; Karyopyknosis; Dense the dying of the block gathering of heterochromatin; Mitochondrion becomes big and becomes that circle, substrate are thin out, ridge shortens and tail off even disappear, and when extreme swelling, is converted into little cavity shape structure, and the cell membrane disrepair phenomenon is arranged.In general, Herba Hedyotidis Diffusae extract is to the effect of the almost non-toxic property of organism normal cell, and can obviously promote mice spleen lymphocytes proliferation, and its effector lymphocyte is B cell, foreign body antigen-specific cytotoxic T lymphocyte.Reports such as Zhou Shi; Oleanolic acid in the Herba Hedyotidis Diffusae (be called for short OA) has antiinflammatory, enhance immunity, inhibition platelet and falls many-sided clinical pharmacology effects such as collection, blood sugar lowering, antioxidation and diuresis, also has biological activitys such as hepatoprotective, lipidemia, atherosclerosis and antitumor.Find in the experiment that in vivo it only has bright inhibitory action to melanoma cells.Herba Hedyotidis Diffusae can be adjusted the expression of mice live body ascites H22 HCC HsPT0 to a certain extent, strengthens its immunogenicity and brings into play antitumor action.

1, HD is to the inhibitory action of Hela cell proliferation

All can suppress the propagation of Hela cell behind the HD effect certain hour of 3 kinds of concentration, its suppression ratio reaches as high as 77.83%.See table 11.

Table 11 variable concentrations HD is to the influence (n=41) of Hela cell proliferation

Compare with matched group D value: * * P＜0.01

2.HD influence to ROS in Hela apoptosis and the cell

With the variable concentrations HD prolongation of action time, the Hela apoptosis rate obviously increases, and the ROS level reduces in the cell.See table 12.

Table 12 variable concentrations HD to Hela cell Kang die with cell in the influence (n=4.

) of ROS

The main chemical compositions of HD has ursolic acid, immune polysaccharide, the acid of bird rope, Herba Hedyotidis Diffusae element etc., and result of study shows that HD has certain anti-tumor activity, external various tumor cell strains is had inhibited proliferation.This research is observed HD in external influence to human cervical carcinoma Hela cell's growth; The result shows that the Hela cell proliferation can be suppressed when HD concentration is 20g/L; Along with the increase and the prolonging of action time of HD concentration jumps; Its inhibitory action is more obvious, shows that HD is to the inhibitory action and the drug level of Hela cell growth with substantial connection is arranged action time.Many chemotherapeutics are realized its antitumor action through the free radical approach, because free-radical generating and removing in the tumor cell are in imbalance state, it is strong to produce the free radical ability, and a little less than the removing ability, very responsive to free radical.ROS comprises the intermediate product with high activity that exists with free radical He do not exist with free radical, and the former has O ₂, OH, the latter has H ₂O ₂, NO etc.Malignant cell can also a certain amount of ROS of autocrine except irriate secretion ROS.The ROS of cell interior generation and the balance of antioxidant Metabolism are depended in the existence of cell, and this balance has reflected redox state level in the cell.Utilize the interior OH level of chemical colorimetry mensuration cell to ask in this experiment and meet reflection O ₂, H ₂O ₂Level; Relation between the ROS in people Hela cell inhibitory effect and the cell is tentatively inquired into; The result shows that HD can reduce ROS level in the Hela cell; Suppress cell proliferation simultaneously, this possibly bring into play the function of removing ROS with HD or block some process that produces ROS relevant in cell.

Radix Notoginseng

Radix Notoginseng is the root of Araliaceae Panax notoginseng (Burk) F.H.Chen, another name Radix Notoginseng, Radix Notoginseng, the hemostasis of having addiction, Repercusion analgesia, the strong effect of tonify deficiency.Its main active is Radix Notoginseng soap two (Panax notoginoside; PNS), many both at home and abroad in recent years researchs have reported that Radix Notoginseng soap two has the immunity of adjusting, improves efficacy effects such as myocardial ischemia, arrhythmia, blood fat reducing, blood pressure lowering, blood sugar lowering, fibrosis, antioxidation, microcirculation improvement, anti-inflammatory and antalgic.This prescription has used functions such as adjusting immunity and the microcirculation improvement of Radix Notoginseng.

1, to the influence of the inductive mouse spleen lymphocyte conversion capability of ConA

The OD difference average of being tried each dose groups of thing all is higher than negative control group, and high dose group and matched group difference has significance (P＜0.01), shows that this is tried the effect that thing has to stimulate the splenocyte increment, transform.

The inductive spleen beautiful jade crust of table 13ConA cell transformation experimental result

1.2 to the mouse cell Immune Effects

The left and right sides ear weight difference of being tried each dose groups mice of thing all is higher than negative control group; The difference of its height, middle dose groups and matched group has significance (P＜0.05 or P＜0.01); Show that this is tried the swelling that thing can significantly improve DNFB inducing mouse ear; Promptly have the effect that promotes delayed allergy, see table 14

Table 14 mice delayed allergy (DTH) experimental result

1.3 influence to mouse antibodies cellulation number

Antibody-producting cell number (plaque number) average of being tried each dose groups mice of thing all is higher than negative control group, and with the difference of matched group significance (P＜0.01 or P＜0.05) is arranged all, explains that this is tried thing and has the value-added effect of enhancing antibody cellulation, sees table 15

Table 15 Hangzhoupro body cellulation test experience result

1.4 influence to mouse humoral immune

The antibody product average of being tried each dose groups mice of thing is higher than negative control group, and wherein the difference of low dose group and matched group has significance (P＜0.05), shows that this is tried thing and has the effect that improves the mice serum hemolysin level.The result sees table 16

The side of table 16 mice serum hemolysin is decided result

1.5 the influence that mouse monokaryon one macrophage carbon cell carbon is cleaned up function

The phagocytic index (a) that is tried each dose groups of thing all is higher than negative control group, and each group all has significance (P＜0.05) with the difference of matched group, shows that this is tried thing and has the mouse monokaryon of promotion one macrophage carbon and clean up effect.See table 17

The little people's carbon of table 17 is cleaned up experimental result

1.6 influence to mouse monokaryon one macrophage phagocytic function

Tried each dose groups Turnover of Mouse Peritoneal Macrophages of thing the phagocytic rate and the phagocytic index of chicken red blood cell all is higher than negative control group; But difference does not have significance; Point out this to be tried thing mouse monokaryon one macrophage phagocytic function is had certain facilitation, but not clearly, see table 18

The real face result

of the huge throat cytophagy of table 18 mouse peritoneal chicken red blood cell

1.7 to the active influence of NK cells in mice

The NK cytoactive of being tried each dose groups mice of thing all is higher than negative control group, and height, middle dose groups and negative control group are relatively, and difference all has significance (P＜0.01), points out this sample to have the NK cells in mice of raising active function.The result sees table 19

Table 19 mice Nh1_ cytoactive side is decided result

This experimental study result shows that Radix Notoginseng soap two has tangible potentiation at aspects such as cellular immune function, humoral immune function and NK cells.

1.8 the Radix Notoginseng alcohol extract is organized the influence of MVD to spleen tumor, sees table 20.

Table 20 Radix Notoginseng alcohol extract is to the influence

of tumor-bearing mice MVD

Compare with matched group.* P＜0.05, * * P＜0.01; Compare △ P＞0.05 with the interferon group

1.9 the Radix Notoginseng alcohol extract is organized the influence of vegf expression to spleen tumor, sees table 21.

The influence (n) of table 21 Radix Notoginseng alcohol extract to making vegf expression

2.0 the Radix Notoginseng alcohol extract is seen table 22 to the influence that spleen kind tumor tissue MNP expresses.

The influence that table 22 Radix Notoginseng alcohol extract is expressed MMP2

Modern medicine study thinks and utilizes angiogenesis inhibitor to suppress vascular endothelial cell proliferation and activity specifically, can suppress growth of tumor and transfer and do not influence other host cell.At present the research of tumor-blood-vessel growth is focused mostly on aspect two, the one, detect the expression of short VEGF (like VEGF) in tissue, the body fluid or Angiostatin, the 2nd, the number of blood vessel in the actual detected tumor tissues.A large amount of research t3] prove that the tumor blood capillary generates the growth of solid tumor and shifts most important; Angiogenesis obviously strengthens before the neoplasm metastasis; The enhancing degree and the neoplasm metastasis ability of its angiogenesis are proportionate, and this shows that tumor tissues must obtain nutrition from the host could be in local growth.Therefore block tumor-blood-vessel growth, cut off tumor tissues and obtain the nutrition approach and become new antineoplaston target spot.This tests discovery, and tumor-bearing mice hepatic metastases clone number increases with the rising of MVD in the NS matched group.And the Radix Notoginseng alcohol extract of various dose all has inhibitory action to MVD, particularly in the Radix Notoginseng, the small dose group inhibitory action is stronger.The Radix Notoginseng alcohol extract is suitable with the interferon action effect to the inhibitory action of MVD.Tumor soma medium vessels ECGF (VEGF) expression becomes positive correlation with its microvessel density and grade malignancy, and apparently higher than nonneoplastic tissue.This shows that VEGF possibly promote tumor to generate through promoting modes such as angiogenesis, and its expression has reflected stimulation, propagation and the transfer of cancerous cell to endotheliocyte.Intervene the effect of VEGF and receptor thereof through antibody or gene level, possibly reach the inhibition tumor-blood-vessel growth, suppress tumor growth and transfer.Mice B16 melanoma liver metastasis model is adopted in this research, this model primary tumo(u)r is organized the influence of vegf expression with Using immunohistochemical Radix Notoginseng alcohol extract.The result shows that the Radix Notoginseng alcohol extract can obviously reduce the expression of the F of inkstone Qiang, and relatively there were significant differences with matched group.Malignant cell is by part tissue infiltration and transfer towards periphery, and these act on the formation that also depends on the infiltration of its pair cell epimatrix and basement membrane (BM) and induce new vessels.ECM is in gene expression, the behavior of regulate tumor cell, influences in its growth, transfer, hypertrophy and the metabolism to play an important role.MMP promotes the infiltration of cancerous cell to surrounding tissue through the degraded to ECM; Also scalable cell and iuntercellular and and substrate between brill attach; Regulate the generation of new vessels, strengthen epithelial motor capacity and promote tumor-blood-vessel growth, thereby promote the infiltration and the transfer of tumor.This result of study shows that the Radix Notoginseng alcohol extract can suppress the expression of the stromatolysis related gene albumen MMP2 of tumor cell, thereby reaches the effect that suppresses invasive ability of tumor cell.

Selenocarrageenan

Selenium is one of nutrient of the definite needed by human of World Health Organization (WHO); Also be that at present various nutrients with immunoloregulation function (comprise that unique and opposing viral infection in the vitamin A, vitamin C/Zn/Mg) have the nutrient of direct relation; It is enhances human body immune system ability in several ways, effectively stops virus mutation, resists multiple disease of viral infection; And can effectively improve people's emotion, have resisting fatigue, anti-depressed effect.Selenium is the important component that constitutes glutathione peroxidase (GPX).Selenium Supplement can make the GPX increased activity, removes free radical and oxidation resistance thereby strengthen, protection body cell film, nucleic acid and proteinic normal configuration and function.

1, selenium is to immune influence

Research shows that selenium influences immunity of organism and mainly comprises 3 kinds of immunization wayses, i.e. cellular immunization, humoral immunization and nonspecific immunity.Selenium can promote the generation of interferon, and can increase the plain activity of γ-disturb external, enhances human body NK cytotoxicity, and do not damage target cell membrane.Selenium can also impel the secretion capacity of lymphocyte IL-1 and IL-2 significantly to strengthen, and the formation of immune stimulatory globulin improves the ability that body synthesizes antibody such as IgG, IgM.In addition, selenium for phagocyte to the chemotactic of virion, engulf and the process of killing all has influence in various degree.Discover, selenium can with the macrophage factor (MAF) the synergistic activation macrophage of living, thereby strengthen anti-tumor activity, can reduce macrophage again to lymphocytic inhibitory action simultaneously.

2, selenic antioxidation is to the inhibition of tumor

The existence of life depends on oxygen, and life entity is through oxidizing process acquisition activity energy needed.From this respect, oxygen is indispensable to life entity.But then, oxygen also possibly cause oxidative damage to life entity, and is described as Diplock: " keeping balance on the cutter ball of life between aspect oxygen metabolism optimum and pernicious." can cause the oxygen of damage to belong to active oxygen to body, free radical is wherein arranged, like ultra-oxygen anion free radical (O ₂ ^-) free radical (HO); Hydroperoxyl radical (HOO), organic peroxy free radical (ROO) etc.; These free radicals comprise that the endogenous free radical and the exogenous chemical factors that are produced by enzyme system in the body or non-enzyme system cause the free radical that produces; But the equal various biomacromolecules of attack are like nucleic acid, protein etc.It not only makes membrane molecule receive oxidative damage, and can change or destroy membrane structure and film function, causes body injury; Or produce biomolecule free radical (secondary free radical) with the living matter effect, and in its subsequent reactions, continue to cause damage, cause the cell biological function reduction, even degeneration causes cancerous protuberance.In the face of so serious free radical threatens; In the organism; Aerobe has formed a cover anti-oxidative defense system in evolution of long period of time, can on different links, block radical reaction, makes life process be able in the balance of the generation of reactive oxygen free radical and removing, normally carry out.In the body antioxidant system, selenium also is an important member.Prove at present; Selenium is the essential ingredient of the sweet skin peroxidase of paddy moon bright (GSH-Px); Contain 4 selenium among each molecule GSH-Px, the GSH-Px ability catalytic reduction type sweet skin of paddy moon bright (GSH) becomes the sweet skin of paddy moon bright (GSSG) of oxidized form, makes deleterious peroxide (ROOH) simultaneously; Be reduced into nontoxicly, and make H through based compound (ROH) ₂O ₂Decompose, the protection cell membrane does not receive the damage of peroxide.

3, selenium is to the influence of mouse tumor weight, tumor weight/body weight index

Visible by table 23, the 25d that continues medication, with the normal control ratio, the selenium of 3 kinds of dosage can both significantly reduce heavy, the tumor weight/body weight of the tumor of tumor-bearing mice, and tumor killing effect is remarkable.

Table 23 selenium is heavy to mouse tumor, the influence

of tumor weight/body weight index

4, selenium is to the influence of macrophage phagocytic function, NK (NK) cytoactive

Can find out that by table 24 continue to selenium 15d and normal control ratio, the selenium of 3 all dosage can both significantly strengthen spleen natural killer cell activity and the peritoneal macrophage of mice and engulf the chicken red blood cell ability.

The nano red essence selenium of table 24 is heavy to mouse tumor, the influence

of tumor weight/body weight index

Microcrystalline Cellulose:

Consider Caulis et Folium Brassicae capitatae, lyophilized Stichopus japonicus powder, Cordyceps, contain sugar and vitamin is higher and the taste medicine does not meet the characteristics of modern's custom; And the operability of the course of processing we selected the food additive microcrystalline Cellulose for use, show in " one one kinds of multi-functional macromolecular materials of microcrystalline Cellulose " literary composition of on " Guangzhou chemical industry " 1993 the 21st the 3rd phases of volume, delivering according to the Zhu Yu of Guangzhou Inst of Chemistry, Chinese Academy of Sciences qin, Tang Liegui, Pan Songhan: microcrystalline Cellulose can be used as antiplastering aid.The microcrystalline Cellulose addition meets GB2760 " food additive use sanitary standard " for 15% of prescription, and microcrystalline Cellulose toxicity property ADI does not do particular provisions (FAO/WHO, 1994); Make its whole prescription have more reasonability and science like this.

This pharmaceutical formulation ratio is: Caulis et Folium Brassicae capitatae 5-12g, Radix Astragali 3.5-10g, Radix Codonopsis 5-15g, Poria 5-15g, Rhizoma Atractylodis Macrocephalae 4-12g, Herba Hedyotidis Diffusae 4-12g, Radix Notoginseng 3-9g, selenocarrageenan 0.007-0.015g, microcrystalline Cellulose 3.6-10g;

Through verification experimental verification; In the wherein above-mentioned prescription ratio; Preferred implementation one is: Caulis et Folium Brassicae capitatae 52g, Radix Astragali 3.5g, Radix Codonopsis 5g, Poria 5g, Rhizoma Atractylodis Macrocephalae 4g, Herba Hedyotidis Diffusae 4g, Radix Notoginseng 3g, selenocarrageenan 0.007g, microcrystalline Cellulose 3.6g, verify the curative effect with obvious excellence by above-mentioned content than the medicine of processing through the test of pesticide effectiveness.

Another preferable embodiment is: Caulis et Folium Brassicae capitatae 12g, Radix Astragali 10g, Radix Codonopsis 15g, Poria 15g, Rhizoma Atractylodis Macrocephalae 12g, Herba Hedyotidis Diffusae 12g, Radix Notoginseng 9g, selenocarrageenan 0.015g, microcrystalline Cellulose 10g, above-mentioned content have the curative effect of obvious excellence through test of pesticide effectiveness checking than the medicine of processing.

An outer in addition preferred implementation is: Caulis et Folium Brassicae capitatae 10g, Radix Astragali 8g, Radix Codonopsis 13g, Poria 13g, Rhizoma Atractylodis Macrocephalae 9g, Herba Hedyotidis Diffusae 9g, Radix Notoginseng 7g, selenocarrageenan 0.009g, microcrystalline Cellulose 8g.Above-mentioned content has the curative effect of obvious excellence through test of pesticide effectiveness checking than the medicine of processing.

Content active constituent content after above-mentioned processing technique distributes as follows in the said medicine: composition: the 100g medicine contains the sweet 375mg of effective composition Radix Raphani sulfur, naphthoquinone 2400mg, crude polysaccharides 8000mg, total saponins 13000mg.Wherein, the effective ingredient in the Caulis et Folium Brassicae capitatae is that sulfur is sweet, and the effective ingredient of the Radix Astragali is a crude polysaccharides; The effective ingredient of Radix Codonopsis is a saponin, and the effective ingredient of Poria is a crude polysaccharides, and the effective ingredient of the Rhizoma Atractylodis Macrocephalae is an atractylone; The effective ingredient of Herba Hedyotidis Diffusae is a naphthoquinone, and the effective ingredient of Radix Notoginseng is a saponin.

A kind of health care sugar-tablet is processed with said medicine, and active constituent content distributes as follows: the 100g medicine contains the sweet 375mg of effective composition Radix Raphani sulfur, naphthoquinone 2400mg, crude polysaccharides 8000mg, total saponins 13000mg.

A kind of health-caring capsule, with the sugar-tablet that above-mentioned health medicine is processed, active constituent content distributes as follows: the 100g medicine contains the sweet 375mg of effective composition Radix Raphani sulfur, naphthoquinone 2400mg, crude polysaccharides 8000mg, total saponins 13000mg.

A kind of method for preparing described health-caring capsule is pulverized the Radix Astragali, Radix Codonopsis, Poria, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, Radix Notoginseng medicine respectively earlier, crosses the 60-80 mesh sieve; The various material powders and other supplementary material that weigh by a certain percentage after the pulverizing mix; Incorporation time control certain hour carries out capsule then and fills, and filling process is according to inner quality standard control implant weight; Screen again, polishing, inner packing, outer package, check warehouse-in at last.

The present invention finds through test; The medicament that is mixed and made into according to the above ratio has the effect of the enhancing human immune slow down aging better than simple one or both compositions, and is able to overcome through the adverse effect that the proportioning of above-mentioned various composition specific proportions has beat all health-care effect and former each composition.

Especially better is that during this production, supplementary material uniform mixing time 45-50 minute capable again tabletting the results showed that the long or too short drug effect for last preparation of the incorporation time of supplementary material has adverse effect.With 45-50 minute was the best.

Several kinds of raw materials are assisted mutually in whole prescription and are deposited mutually more than this specific embodiment, bring out the best in each other, and make full side present effect preferably, and product has tangible enhancing immunity, alleviating physical fatigue, anti-tumor function effect through these article of function test proof.

Claims

1. a health medicine comprises Caulis et Folium Brassicae capitatae 5-12g, Radix Astragali 3.5-10g, Radix Codonopsis 5-15g, Poria 5-15g, Rhizoma Atractylodis Macrocephalae 4-12g, Herba Hedyotidis Diffusae 4-12g, Radix Notoginseng 3-9g, selenocarrageenan 0.007-0.015g, microcrystalline Cellulose 3.6-10g.

2. health medicine according to claim 1 is characterized in that, each component ratio is: Caulis et Folium Brassicae capitatae 52g, Radix Astragali 3.5g, Radix Codonopsis 5g, Poria 5g, Rhizoma Atractylodis Macrocephalae 4g, Herba Hedyotidis Diffusae 4g, Radix Notoginseng 3g, selenocarrageenan 0.007g, microcrystalline Cellulose 3.6g.

3. health medicine according to claim 1; It is characterized in that each component ratio is: Caulis et Folium Brassicae capitatae 12g, Radix Astragali 10g, Radix Codonopsis 15g, Poria 15g, Rhizoma Atractylodis Macrocephalae 12g, Herba Hedyotidis Diffusae 12g, Radix Notoginseng 9g, selenocarrageenan 0.015g, microcrystalline Cellulose 10g.

4. health medicine according to claim 1; It is characterized in that each component ratio is: Caulis et Folium Brassicae capitatae 10g, Radix Astragali 8g, Radix Codonopsis 13g, Poria 13g, Rhizoma Atractylodis Macrocephalae 9g, Herba Hedyotidis Diffusae 9g, Radix Notoginseng 7g, selenocarrageenan 0.009g, microcrystalline Cellulose 8g.

One kind the health care sugar-tablet; The sugar-tablet of processing with each described health medicine among the claim 1-4 is characterized in that active constituent content distributes as follows: the 100g medicine contains the sweet 375mg of effective composition Radix Raphani sulfur, naphthoquinone 2400mg, crude polysaccharides 8000mg, total saponins 13000mg.

6. health-caring capsule; The sugar-tablet of processing with each described health medicine among the claim 1-4 is characterized in that active constituent content distributes as follows: the 100g medicine contains the sweet 375mg of effective composition Radix Raphani sulfur, naphthoquinone 2400mg, crude polysaccharides 8000mg, total saponins 13000mg.

7. method for preparing the health-caring capsule described in the claim 6; It is characterized in that, earlier the Radix Astragali, Radix Codonopsis, Poria, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, Radix Notoginseng medicine are pulverized respectively, cross the 60-80 mesh sieve; The various material powders and other supplementary material that weigh by a certain percentage after the pulverizing mix; Incorporation time control certain hour carries out capsule then and fills, and filling process is according to inner quality standard control implant weight; Screen again, polishing, inner packing, outer package, check warehouse-in at last.

8. method according to claim 7 is characterized in that, incorporation time is 45-50 minute.