CN102511790B - Preparation method of aquatic protein peptide microcapsules - Google Patents
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- CN102511790B CN102511790B CN2011103958841A CN201110395884A CN102511790B CN 102511790 B CN102511790 B CN 102511790B CN 2011103958841 A CN2011103958841 A CN 2011103958841A CN 201110395884 A CN201110395884 A CN 201110395884A CN 102511790 B CN102511790 B CN 102511790B
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 44
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 43
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 43
- 239000003094 microcapsule Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000011162 core material Substances 0.000 claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 23
- 108010010803 Gelatin Proteins 0.000 claims abstract description 9
- 229920000084 Gum arabic Polymers 0.000 claims abstract description 9
- 235000010489 acacia gum Nutrition 0.000 claims abstract description 9
- 239000000205 acacia gum Substances 0.000 claims abstract description 9
- 229920000159 gelatin Polymers 0.000 claims abstract description 9
- 239000008273 gelatin Substances 0.000 claims abstract description 9
- 235000019322 gelatine Nutrition 0.000 claims abstract description 9
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 9
- 235000010493 xanthan gum Nutrition 0.000 claims abstract description 8
- 229920001285 xanthan gum Polymers 0.000 claims abstract description 8
- 239000000230 xanthan gum Substances 0.000 claims abstract description 8
- 229940082509 xanthan gum Drugs 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims description 12
- 241000978776 Senegalia senegal Species 0.000 claims description 8
- 238000001694 spray drying Methods 0.000 claims description 7
- 238000004945 emulsification Methods 0.000 claims description 6
- 238000000265 homogenisation Methods 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 241000242583 Scyphozoa Species 0.000 claims description 5
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 6
- 230000029087 digestion Effects 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000019629 palatability Nutrition 0.000 abstract description 3
- 239000002253 acid Substances 0.000 abstract description 2
- 238000010298 pulverizing process Methods 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract 1
- 238000005303 weighing Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 10
- 235000019658 bitter taste Nutrition 0.000 description 4
- 241000237509 Patinopecten sp. Species 0.000 description 3
- 241001125048 Sardina Species 0.000 description 3
- 235000019512 sardine Nutrition 0.000 description 3
- 235000020637 scallop Nutrition 0.000 description 3
- 238000005538 encapsulation Methods 0.000 description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 210000001779 taste bud Anatomy 0.000 description 1
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Abstract
本发明涉及一种水产蛋白肽微胶囊的制备方法,其是选取干燥的水产蛋白肽作为芯材,超微粉碎处理;称取明胶、阿拉伯胶和黄原胶分别按质量分数为0.5-1%、1-2%、0.5%-0.8%加水完全溶解,等比例混合后作为壁材;将壁材与芯材按照质量比为6﹕1-7﹕1的比例混合,乳化均质处理;将乳化均质处理后的壁材与芯材喷雾干燥,得水产蛋白肽微胶囊。按本发明的制备方法得到的微胶囊包埋率高、适口性好、酸稳定性好、缓释,且不影响肽的消化吸收。其制备方法不需新增设备,工艺合理,操作简便,易实现工业化生产。The invention relates to a preparation method of aquatic protein peptide microcapsules, which comprises selecting dried aquatic protein peptide as a core material and superfine pulverizing treatment; weighing gelatin, acacia gum and xanthan gum to be 0.5-1% by mass fraction respectively , 1-2%, 0.5%-0.8% are completely dissolved in water, and mixed in equal proportions as the wall material; the wall material and the core material are mixed according to the mass ratio of 6:1-7:1, emulsified and homogenized; The emulsified and homogenized wall material and core material are spray-dried to obtain aquatic protein peptide microcapsules. The microcapsules obtained by the preparation method of the invention have high embedding rate, good palatability, good acid stability, slow release, and do not affect the digestion and absorption of peptides. The preparation method does not need additional equipment, has reasonable technology, simple and convenient operation, and is easy to realize industrialized production.
Description
技术领域 technical field
本发明涉及一种食用蛋白质的加工,尤其是一种水产蛋白肽微胶囊的制备方法。 The invention relates to a processing of edible protein, in particular to a preparation method of aquatic protein peptide microcapsules.
背景技术 Background technique
我们知道,蛋白肽是将蛋白质进行酶解或发酵之后产生的物质,其具有多种生物 活性。蛋白肽比蛋白质更容易被人体吸收利用,可不经过消化分解,直接以完整的结构进入人体,快速地发挥其生理功能。 We know that protein peptides are substances produced after enzymatic hydrolysis or fermentation of proteins, which have various biological activities. Protein peptides are easier to be absorbed and utilized by the human body than proteins, and can directly enter the human body with a complete structure without digestion and decomposition, and quickly exert their physiological functions.
天然蛋白质疏水性结构常常被掩蔽在天然蛋白质分子内部,在经酶水解成相对分子质量低于6000Da的多肽时,疏水性结构就会较多暴露而出,这些暴露出结构与味蕾发生接触,因而产生苦味。直接作为食品原料会严重影响产品的感官品质。另外,这一类苦味肽因具有疏水性基团,稳定性较差,多肽干粉的吸湿性强,不易贮藏。因此,苦味和稳定性是制约活性肽产品开发的重要因素。 The hydrophobic structure of natural protein is often masked inside the natural protein molecule. When it is hydrolyzed into a polypeptide with a relative molecular weight below 6000Da by enzymatic hydrolysis, more hydrophobic structures will be exposed. These exposed structures come into contact with taste buds, so Produces a bitter taste. Direct use as food raw materials will seriously affect the sensory quality of the product. In addition, this type of bitter peptide has poor stability due to its hydrophobic groups, and the dry peptide powder has strong hygroscopicity and is not easy to store. Therefore, bitterness and stability are important factors restricting the development of active peptide products.
如何减轻蛋白肽的苦味及增加稳定性是一个亟待解决的技术问题。 How to reduce the bitterness and increase the stability of protein peptides is a technical problem to be solved urgently.
发明内容 Contents of the invention
为了克服现有技术中的不足,本发明提供一种工艺合理、操作简便的水产蛋白肽微胶囊的制备工艺。采用该制备方法所得到的水产蛋白肽微胶囊,其包埋率高,适口性好,酸性条件下稳定、缓释,并且不影响肽的消化吸收。 In order to overcome the deficiencies in the prior art, the present invention provides a process for preparing aquatic protein peptide microcapsules with reasonable process and easy operation. The aquatic protein peptide microcapsule obtained by the preparation method has high embedding rate, good palatability, stable and sustained release under acidic conditions, and does not affect the digestion and absorption of the peptide.
本发明解决其技术问题所采用的技术方案是:一种水产蛋白肽微胶囊的制备方法,其特征在于:经过下列工艺步骤 The technical scheme adopted by the present invention to solve its technical problems is: a preparation method of aquatic protein peptide microcapsules, which is characterized in that: through the following process steps
选取干燥的水产蛋白肽作为芯材,超微粉碎,过100目筛,备用; Select dried aquatic protein peptides as the core material, ultrafinely pulverize, pass through a 100-mesh sieve, and set aside;
称取明胶、阿拉伯胶和黄原胶分别加水完全溶解,等比例混合后作为壁材;其中,所述的明胶的质量分数为0.5-1%,阿拉伯胶的质量分数为1-2%, 黄原胶的质量分数为0.5%-0.8%; Weigh gelatin, gum arabic and xanthan gum and add water to dissolve them completely respectively, mix them in equal proportions and use them as wall materials; wherein, the mass fraction of gelatin is 0.5-1%, the mass fraction of gum arabic is 1-2%, yellow The mass fraction of raw gum is 0.5%-0.8%;
将壁材与芯材按照质量比为6﹕1-7﹕1的比例混合,进行乳化均质处理; Mix the wall material and the core material according to the mass ratio of 6:1-7:1, and carry out emulsification and homogenization treatment;
将乳化均质处理后的壁材与芯材喷雾干燥,得水产蛋白肽微胶囊;其中喷雾干燥的条件:进风温度180℃-200℃、出风温度80℃-90℃、进料速度30-50mL/min。 Spray-dry the emulsified and homogenized wall material and core material to obtain aquatic protein peptide microcapsules; the spray-drying conditions: air inlet temperature 180°C-200°C, air outlet temperature 80°C-90°C, feed rate 30°C -50mL/min.
所述芯材是各种水产蛋白水解所得到具有生理活性的蛋白肽。 The core material is a protein peptide with physiological activity obtained by hydrolysis of various aquatic proteins.
本发明采用超微粉碎法对水产蛋白肽芯材进行粉碎处理,并采用明胶、阿拉伯胶、黄原胶的复合物作为复合壁材,通过喷雾干燥法以壁材包裹芯材,形成包埋率高、适口性好、酸稳定性好、缓释的水产蛋白肽微胶囊制品。经测定:所制备的微胶囊的包埋率在90%以上。本发明是将水产蛋白肽进行微胶囊处理,掩盖了其苦而不破坏蛋白肽的原始结构和生物学活性味,不影响肽的消化吸收。本发明在完成干燥工艺的同时进行包埋处理,不需要新增设备,其工艺合理、操作简便、易实现工业化生产。 In the present invention, the core material of the aquatic protein peptide is pulverized by an ultrafine pulverization method, and a compound of gelatin, gum arabic, and xanthan gum is used as a composite wall material, and the core material is wrapped with the wall material by a spray drying method to form an embedding rate. High quality, good palatability, good acid stability, slow-release aquatic protein peptide microcapsules. It has been determined that the embedding rate of the prepared microcapsules is above 90%. The invention microencapsulates the aquatic protein peptide to cover up its bitterness without destroying the original structure and biologically active taste of the protein peptide, and does not affect the digestion and absorption of the peptide. The invention performs embedding treatment while completing the drying process, does not need additional equipment, has reasonable process, simple and convenient operation, and is easy to realize industrialized production.
具体实施方式 Detailed ways
下面结合实施例对本发明做进一步说明。 The present invention will be further described below in conjunction with embodiment.
实施例1 Example 1
一种水产蛋白肽微胶囊的制备方法,经过下列工艺步骤: A preparation method of aquatic protein peptide microcapsules, through the following process steps:
选取干燥的海蜇蛋白肽粉作为芯材,超微粉碎,过100目筛,备用; Select dried jellyfish protein peptide powder as the core material, ultrafinely pulverize, pass through a 100-mesh sieve, and set aside;
称取明胶、阿拉伯胶和黄原胶分别按质量分数0.5%、1%、0.5%加水完全溶解,等比例混合,作为壁材; Weigh gelatin, gum arabic and xanthan gum according to the mass fraction of 0.5%, 1%, 0.5% and add water to dissolve completely, mix in equal proportions, and use them as wall materials;
将壁材与芯材按照质量比为6﹕1的比例混合,进行乳化均质处理; Mix the wall material and the core material according to the mass ratio of 6:1 for emulsification and homogenization;
将乳化均质处理后的壁材与芯材喷雾干燥,得海蜇蛋白肽微胶囊;其中喷雾干燥的条件:进风温度180℃、出风温度80℃、进料速度50mL/min。 The emulsified and homogenized wall material and core material were spray-dried to obtain jellyfish protein peptide microcapsules; the spray-drying conditions were: inlet air temperature 180°C, outlet air temperature 80°C, feed rate 50mL/min.
按此制备方法所得到的海蜇蛋白肽微胶囊包埋率为90%,且在pH3.0稳定性好。 The encapsulation rate of the jellyfish protein peptide microcapsule obtained by this preparation method is 90%, and the stability is good at pH 3.0.
实施例2 Example 2
一种水产蛋白肽微胶囊的制备方法,经过下列工艺步骤: A preparation method of aquatic protein peptide microcapsules, through the following process steps:
选取干燥的鲍鱼蛋白肽粉作为芯材,超微粉碎,过100目筛,备用; Select dried abalone protein peptide powder as the core material, ultrafinely pulverize, pass through a 100-mesh sieve, and set aside;
称取明胶、阿拉伯胶和黄原胶分别按质量分数1%、2%、0.8%加水完全溶解,等比例混合,作为壁材; Weigh gelatin, gum arabic and xanthan gum according to the mass fraction of 1%, 2% and 0.8% respectively and add water to completely dissolve them, and mix them in equal proportions as wall materials;
将壁材与芯材按照质量比为7﹕1的比例混合,进行乳化均质处理; Mix the wall material and the core material according to the mass ratio of 7:1 for emulsification and homogenization;
将乳化均质处理后的壁材与芯材喷雾干燥,得鲍鱼蛋白肽微胶囊;其中喷雾干燥的条件:进风温度185℃、出风温度85℃、进料速度40mL/min。 The emulsified and homogenized wall material and core material were spray-dried to obtain abalone protein peptide microcapsules; the spray-drying conditions were: inlet air temperature 185°C, outlet air temperature 85°C, feed rate 40mL/min.
按此制备方法所得到的鲍鱼蛋白肽微胶囊包埋率为92%以上,且在pH3.0稳定性好。 The embedding rate of the abalone protein peptide microcapsules obtained by the preparation method is over 92%, and the stability is good at pH 3.0.
实施例3 Example 3
一种水产蛋白肽微胶囊的制备方法,经过下列工艺步骤: A preparation method of aquatic protein peptide microcapsules, through the following process steps:
选取干燥的沙丁鱼蛋白肽粉作为芯材,超微粉碎,过100目筛,备用; Select dry sardine protein peptide powder as the core material, ultrafinely pulverize, pass through a 100-mesh sieve, and set aside;
称取明胶、阿拉伯胶和黄原胶分别按质量分数1%、1%、0.8%加水完全溶解,等比例混合,作为壁材; Weigh gelatin, gum arabic and xanthan gum according to the mass fraction of 1%, 1% and 0.8% respectively and add water to completely dissolve them, and mix them in equal proportions as wall materials;
将壁材与芯材按照质量比为6﹕1的比例混合,进行乳化均质处理; Mix the wall material and the core material according to the mass ratio of 6:1 for emulsification and homogenization;
将乳化均质处理后的壁材与芯材喷雾干燥,得沙丁鱼蛋白肽微胶囊;其中喷雾干燥的条件:进风温度190℃、出风温度90℃、进料速度30mL/min。 The emulsified and homogenized wall material and core material were spray-dried to obtain sardine protein peptide microcapsules; the spray-drying conditions were: inlet air temperature 190°C, outlet air temperature 90°C, feed rate 30mL/min.
按此制备方法所得到的沙丁鱼蛋白肽微胶囊包埋率为91%,且在pH3.0稳定性好。 The embedding rate of the sardine protein peptide microcapsule obtained by this preparation method is 91%, and the stability is good at pH 3.0.
实施例4 Example 4
一种水产蛋白肽微胶囊的制备方法,经过下列工艺步骤: A preparation method of aquatic protein peptide microcapsules, through the following process steps:
选取干燥的扇贝蛋白肽粉作为芯材,超微粉碎,过100目筛,备用; Select dried scallop protein peptide powder as the core material, ultrafinely pulverize, pass through a 100-mesh sieve, and set aside;
称取明胶、阿拉伯胶和黄原胶分别按质量分数0.8%、1.5%、0.7%加水完全溶解,等比例混合,作为壁材; Weigh gelatin, gum arabic and xanthan gum according to the mass fraction of 0.8%, 1.5%, 0.7% and add water to dissolve completely, mix in equal proportions, and use them as wall materials;
将壁材与芯材按照质量比为6.5﹕1的比例混合,进行乳化均质处理; Mix the wall material and the core material according to the mass ratio of 6.5:1, and carry out emulsification and homogenization treatment;
将乳化均质处理后的壁材与芯材喷雾干燥,得扇贝蛋白肽微胶囊;其中喷雾干燥的条件:进风温度200℃、出风温度90℃、进料速度50mL/min。 The emulsified and homogenized wall material and core material were spray-dried to obtain scallop protein peptide microcapsules; the spray-drying conditions were: inlet air temperature 200°C, outlet air temperature 90°C, feed rate 50mL/min.
按此制备方法所得到的扇贝蛋白肽微胶囊包埋率为91.5%,且在pH3.0稳定性好。 The encapsulation rate of the scallop protein peptide microcapsule obtained by this preparation method is 91.5%, and the stability is good at pH 3.0.
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CN1206942C (en) * | 2002-04-23 | 2005-06-22 | 李兆明 | Health food compounded with oyster and scallop enzymolzing liquid and soybean lecithin |
CN1202749C (en) * | 2002-08-30 | 2005-05-25 | 大连轻工业学院 | Abalone food and its preparation method |
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