CN102499635A - Line scanning-based fundus retina multispectral imaging system and method - Google Patents

Abstract

The invention relates to a line scanning-based fundus retina multispectral imaging system and a method, which apply the multispectral imaging technology to a line scanning confocal imaging technology, illuminate the fundus retina by scanning line beams with a plurality of wavelengths, simultaneously split the line beams reflected by the fundus retina by using a grating, and detect and image the split sequence spectrum by a detection device. The invention can obtain multispectral images of fundus retina illuminated by multiple wavelengths simultaneously, can multiply the information quantity of the fundus retina compared with single-wavelength imaging, adopts a line beam confocal scanning mode, and has the advantages of fast imaging frame frequency, high resolution, simple and convenient system and the like.

Description

A system and method for multispectral imaging of fundus and retina based on line scanning

technical field

The invention belongs to the multispectral imaging technology in applied optics, and is a fundus retinal multispectral imaging system and method based on line scanning, which can be widely used in biomedical ophthalmological examination.

Background technique

The successful application of optical confocal scanning technology in imaging has broken through the bottleneck of traditional optical imaging, and can obtain high-resolution imaging, making confocal scanning imaging technology applicable to all aspects of optical inspection. With the development of confocal technology, point scanning confocal imaging method and line scanning confocal imaging method have been popularized and applied. Point-scanning confocal imaging requires two-dimensional scanning point beams to illuminate the entire field of view, and at the same time uses weak photodetectors (such as photomultiplier tubes) for detection and imaging. This method has disadvantages such as difficult scanning control, low imaging frame rate, and low detection sensitivity. Line-scanning confocal imaging only requires one-dimensional scanning line beams to illuminate the entire field of view, and a line detector is used for detection and imaging. This method has easy scanning control, high detection sensitivity, and high imaging frame rate, which is more advantageous than the previous method. However, since they all use a single single-band laser as a light source, they can only obtain imaging patterns of a single wavelength. For the study of the spectral characteristics of the detected object, it is necessary to change multiple wavelengths for multiple imaging, which limits its application.

The imaging spectroscopy technology that has emerged in recent years organically combines spectral analysis technology with two-dimensional imaging technology, which can not only perform two-dimensional morphology imaging of objects, but also provide rich spectral information. Since each pixel in the spectral image data contains spectral information related to the measured physical components, it can directly reflect the physical spectral characteristics of the target, thereby revealing the existence and material composition of various targets, making it possible to directly identify the target from space. become possible. Due to its high spectral resolution, multiple bands, and the combination of images and spectra, it is widely used in the detection of space and the earth in remote sensing systems, the inspection of components and lesions in living tissue in biomedicine, military target detection, and food safety. It has a wide range of applications in detection and other aspects.

At present, in the multispectral imaging technology, the divergent beam of the broadband light source is mostly used to focus the sample in a point shape, and then the imaging beam reflected or transmitted back from the sample is separated into a sequence of spectral lines by using the dispersion characteristics of the spectroscopic system, and finally the sequence of spectral lines is analyzed. Simultaneous detection to enable simultaneous multispectral imaging of the same sample. In the point illumination method, it is necessary to scan the light spot to realize the illumination of the entire sample field of view, or to move the sample illumination, which requires two-dimensional precise control, many moving parts, difficult control, and slow imaging speed.

Contents of the invention

The purpose of the present invention is to compensate the deficiencies of the above-mentioned existing two technologies, better meet the requirements of multispectral imaging technology, and enhance the application range of line scanning confocal imaging technology at the same time, and to find a combination of imaging spectrum technology and Line-scanning confocal techniques are combined to form a line-scan-based method for fundus retinal multispectral imaging and a system for implementing this method is provided.

The fundus retinal multispectral imaging system based on line scanning of the present invention includes a line beam generating module, a spectroscopic module, a scanning module, an imaging module and an output module, and is characterized in that,

The line beam generating module includes a light source, and the light source can be a single-band point light source group or a broadband point light source;

The imaging module includes a detection device, which may be a line detector group or an area detector.

When the light source is a single-band point light source group, the line beam generation module also includes a fiber coupler, a collimator lens and a cylindrical lens. After the outgoing beam of the single-band point light source group is coupled into a single beam by the fiber coupler, the single beam Sequentially collimated by a collimator lens and transformed into a line beam by a cylindrical lens; when the light source is a broadband point light source, the line beam generation module also includes a collimator lens and a cylindrical lens, and the outgoing beam of the broadband point light source passes through the collimator lens in turn The collimation and the cylindrical lens are transformed into a line beam; the line beam generation module is used to generate a one-dimensional line beam from the divergent beam output by the light source, and the line beam generation module is connected with the light splitting module;

The spectroscopic module is a broadband spectroscopic flat plate or a broadband spectroscopic prism, which is used to directly transmit a part of the one-dimensional line beam generated by the line beam generating module to the scanning module, and deflect the imaging beam reflected from the scanning module to reach the imaging beam module;

The scanning module uses the line beam directly emitted by the spectroscopic module to scan and illuminate the retina of the human eye, and synchronously reflects the imaging beam reflected from the retina to the spectroscopic module, which is composed of a scanning galvanometer and an illumination objective lens group. The directly emitted one-dimensional line beam passes through the scanning galvanometer and the illumination objective lens group in turn, and the scanning galvanometer scans and illuminates the fundus retina, and the imaging beam reflected from the fundus retina sequentially passes through the illumination objective lens group and the scanning oscillating mirror to reach the spectroscopic module;

The imaging module is used to convert the light intensity signal of the imaging beam deflected and emitted by the spectroscopic module into an electrical signal and transmit it to the output module. The imaging module is composed of an imaging objective lens, a cylindrical lens, a confocal slit, a grating and a detection device. The imaging beam deflected and emitted by the spectroscopic module passes through the imaging objective lens, cylindrical lens, confocal slit and grating in sequence, and reaches the detection device. The confocal slit is conjugate to the retinal plane of the fundus;

The output module is composed of an image acquisition card and an output device. The image acquisition card converts the electrical signal output by the imaging module into an image signal and outputs it through the output device.

The light source is a laser light source, or a light emitting diode, or a superluminescent light emitting diode;

When the detection device is a line detector group, the line detector is a linear charge-coupled device, or a linear complementary metal oxide semiconductor array, or a linear photodiode array; when the detection device is a surface detector, the surface detection The device is an area array charge-coupled device, or an area array complementary metal oxide semiconductor array, or an area array photodiode array.

The fiber coupler is an N*1 type coupler, and N represents the number of output beams of the point light source group;

The collimating lens is an achromatic lens, which is used to collimate the divergent light beam into a parallel light beam;

The cylindrical lens is used to transform the parallel beam output by the collimating lens into a one-dimensional line beam;

The scanning galvanometer is a reflective high-precision scanning galvanometer;

The illumination objective lens group is a beam reduction subsystem composed of two lenses;

The grating is a diffraction grating or a holographic grating;

The output device is a computer.

The fundus retinal multispectral imaging method based on line scanning of the present invention is characterized in that the realization steps are as follows:

Step 1, the line beam generation module generates a single line beam containing multiple wavelengths;

Step 2, a part of the single-beam line beam containing multiple wavelengths is directly transmitted to the scanning module after passing through the spectroscopic module;

Step 3, the scanning module scans and illuminates the retinal retina of the human eye with the line beam directly emitted by the spectroscopic module through the scanning galvanometer and the illumination objective lens group, and synchronously reflects the imaging beam reflected from the retinal retina to the spectroscopic module;

In step 4, the beam splitting module deflects the imaging light beam reflected from the scanning module and sends it to the imaging module.

Step 5, the imaging module converts the light intensity signal of the imaging beam deflected and emitted by the spectroscopic module into an electrical signal, and transmits it to the output module;

Step 6, the image acquisition card in the output module converts the electrical signal output by the imaging module into an image signal, and outputs it through the output device.

When the light source is a single-band point light source group, the step 1 includes,

Step 111, the multiple divergent beams emitted by the single-band point light source group are coupled by the fiber coupler, and output as a single beam containing multiple wavelengths;

Step 112, the collimating lens collimates the single beam containing multiple wavelengths into a parallel beam;

In step 113, the parallel beam is transformed into a line beam through a cylindrical lens.

When the light source is a broadband point light source, the step 1 includes,

Step 121, collimating the divergent beam emitted by the broadband point light source into a parallel beam after passing through the collimating lens;

In step 122, the parallel beam is transformed into a line beam through a cylindrical lens.

Said step 5 includes,

Step 51, the imaging objective lens and the cylindrical lens focus the imaging beam deflected and emitted by the spectroscopic module into a line, and then reach the grating;

Step 52, the line beam is diffracted by the grating into separate levels of single-wavelength line beams;

Step 53, the separated single-wavelength light beams of various levels reach the detection device, and the detection device converts the light intensity signal into an electrical signal.

Said step 6 includes,

Step 61, the image acquisition card converts the electrical signal output by the detection device into an image signal;

Step 62, the output device receives the image signal, displays, processes, stores and prints it.

Compared with the existing multispectral imaging technology, the present invention has the following advantages:

1. The line-scan-based fundus retinal multispectral imaging system and method of the present invention can scan and illuminate the fundus retina with line beams including multiple bands, and then perform detection and imaging of each band at the same time through grating splitting, so as to obtain multispectral images of retinal tissue. information.

2. The fundus retinal multispectral imaging system and method based on line scanning of the present invention, the confocal slit and the fundus retina are conjugated, which eliminates the influence of stray light on the imaging quality of the non-retinal conjugate surface, and realizes the high efficiency of the confocal imaging principle. resolution.

3. The fundus retinal multispectral imaging system and method based on line scanning of the present invention, the scanning beam and the imaging beam are both linear, and only one scanning galvanometer is used to scan the beam to illuminate the field of view of the fundus retina, with fewer moving parts and high control accuracy , and the clock synchronization between the scanning galvanometer and the line detector is simple, the image construction speed is fast, the image frame frequency is high, and the real-time performance is good.

Description of drawings

Fig. 1 is a structure diagram of the fundus retinal multispectral imaging system and method based on line scanning in the present invention.

FIG. 2 is a structural diagram of a system and method for fundus retinal multispectral imaging based on line scanning according to a specific embodiment of the present invention.

FIG. 3 is a schematic diagram of the optical path of a line-scan-based fundus retinal multispectral imaging system and method according to a specific embodiment of the present invention.

Fig. 4 is a structural diagram of a fundus retinal multispectral imaging system based on line scanning according to Embodiment 2 of the present invention.

5 is a schematic diagram of the optical path of the line-scan-based fundus retinal multispectral imaging system and method according to Embodiment 2 of the present invention.

Detailed ways

The present invention will be described in further detail below through specific embodiments and in conjunction with the accompanying drawings.

Embodiment one

As shown in FIG. 2 , it is a structural diagram of a line-scan-based fundus retinal multispectral imaging system and method according to a specific embodiment of the present invention, including a line beam generating module 1, a spectroscopic module 2, a scanning module 3, an imaging module 5 and an output module 6 .

The line beam generation module 1 is connected to the light splitting module 2, and is composed of a single-band point light source group 100, a fiber coupler 110, a collimator lens 120 and a cylindrical lens 130, and is used to diverge the multiple wavelength beams of the single-band point light source group 100 A one-dimensional line beam is generated. The beam emitted by the single-band point light source group 100 is coupled into a single beam through the fiber coupler 110. The single beam is collimated by the collimator lens 120 and then outputs a parallel beam. The cylindrical lens 130 will collimate the lens The parallel light beam output by 120 is transformed into a one-dimensional line beam and output to the spectroscopic module 2 .

The spectroscopic module 2 is a broadband spectroscopic flat plate or a broadband spectroscopic prism, which is used to directly transmit a part of the one-dimensional line beam generated by the line beam generating module 1 to the scanning module 3, and deflect the imaging beam reflected from the scanning module 3 to reach the scanning module 3. Imaging module 5.

The scanning module 3 is composed of a scanning galvanometer 300 and an illumination objective lens group 310, and is used for scanning and illuminating the retina 4 with the line beam directly emitted by the spectroscopic module 2, and synchronously reflecting the imaging beam reflected from the retina 4 to the spectroscopic module 2 . The line beam directly emitted by the spectroscopic module 2 passes through the scanning galvanometer 300 and the illumination objective lens group 310 in sequence, and the scanning galvanometer scans and illuminates the retina 4 of the human eye, and the imaging beam reflected from the human eye retina 4 passes through the illumination objective lens group 310 and the scanning galvanometer 300 in sequence Synchronous reflection to the splitting module 2.

The imaging module 5 is composed of an imaging objective lens 500, a cylindrical lens 510, a confocal slit 520, a grating 530 and a detection device 540, and is used to convert the light intensity signal of the imaging beam deflected by the spectroscopic module 2 into an electrical signal, and transmit it to Output module 6. The imaging light beam deflected by the spectroscopic module 2 passes through the imaging objective lens 500 , the cylindrical lens 510 , the confocal slit 520 and the grating 530 in sequence, and reaches the detection device 540 . The confocal slit 520 is conjugate to the plane of the retina 4 of the human eye, and the confocal slit 520 can exclude stray light that is not on the plane of the retina 4 of the human eye from entering the detection device 540 , thereby realizing the high resolution of the confocal imaging principle.

The output module 6 is composed of an image acquisition card 600 and an output device 610 , the image acquisition card 600 converts the electrical signal output by the imaging module 5 into an image signal, and outputs it through the output device 610 .

As shown in Figure 3, it is a schematic diagram of the optical path of a specific embodiment of the present invention-a line-scan-based fundus retinal multispectral imaging system and method, and three beams are representatively drawn, all components are arranged along the principal optical axis, and the aperture of the light, etc. High concentricity, the light beams all propagate along the main optical axis of the system. The figures are schematic in nature and do not represent real optical design parameters.

The single-band point light source group is composed of multiple single-band point light sources. The number of point light sources and wave bands can be selected and combined arbitrarily.

The point light source is a laser light source, or a light emitting diode, or a superluminescent light emitting diode, and a laser light source is used in this embodiment.

The fiber coupler is an N*1 type coupler, and N represents the number of output beams of the point light source group.

The collimating lens is an achromatic lens with a focal length of 50mm.

The cylindrical lens is a common plano-convex cylindrical lens with a focal length of 100mm.

The beam splitter is a broadband beam splitting plate or a broadband beam splitting prism, and a broadband beam splitting prism is used in this embodiment.

The scanning galvanometer is a reflective high-precision scanning galvanometer. In this embodiment, the model 6210H product of CT Company is used, and the effective surface width is 10 mm.

The illumination objective lens group is a beam reduction subsystem composed of two lenses. In this embodiment, two doublet lenses with focal lengths of 100 mm and 50 mm are used to form the beam reduction subsystem.

The imaging objective lens 7 adopts a lens whose focal length is 140mm.

The confocal slit is an adjustable slit. In this embodiment, the APAS80-1A of Beijing Zhuo Li Hanguang is used, and its position is conjugate to the plane of the retina of the fundus.

The grating is a diffraction grating or a holographic grating. In this embodiment, Thorlabs' broadband diffraction grating is used, and the number of line pairs is 300 per millimeter.

The line detector is a linear charge-coupled device, or a linear CMOS array, or a linear photodiode array. In this embodiment, a linear CCD from e2v Company is used, with 1024 line pixels and a pixel size of 14 μm×14 μm.

Embodiment two

As shown in FIG. 4 , it is a structural diagram of a line-scan-based fundus retinal multispectral imaging system and method according to Embodiment 2 of the present invention, including a line beam generating module 1, a spectroscopic module 2, a scanning module 3, an imaging module 5, and an output module 6. .

The line beam generating module 1 is connected to the spectroscopic module 2 and consists of a broadband point light source 100 , a collimator lens 110 and a cylindrical lens 120 , and is used to generate a one-dimensional line beam from multiple wavelength divergent beams of the broadband point light source 100 . The divergent light beam emitted by the broadband point light source 100 is collimated by the collimator lens 110 to output a parallel beam.

The spectroscopic module 2 is a broadband spectroscopic flat plate or a broadband spectroscopic prism, which is used to directly transmit a part of the one-dimensional line beam generated by the line beam generating module 1 to the scanning module 3, and deflect the imaging beam reflected from the scanning module 3 to reach the scanning module 3. Imaging module 5.

The scanning module 3 is composed of a scanning galvanometer 300 and an illumination objective lens group 310, and is used for scanning and illuminating the retina 4 with the line beam directly emitted by the spectroscopic module 2, and synchronously reflecting the imaging beam reflected from the retina 4 to the spectroscopic module 2 . The line beam directly emitted by the spectroscopic module 2 passes through the scanning galvanometer 300 and the illumination objective lens group 310 in sequence, and the scanning galvanometer scans and illuminates the retina 4 of the human eye, and the imaging beam reflected from the human eye retina 4 passes through the illumination objective lens group 310 and the scanning galvanometer 300 in sequence Synchronous reflection to the splitting module 2.

The imaging module 5 is composed of an imaging objective lens 500, a cylindrical lens 510, a confocal slit 520, a grating 530 and a detection device 540, and is used to convert the light intensity signal of the imaging beam deflected by the spectroscopic module 2 into an electrical signal, and transmit it to Output module 6. The imaging light beam deflected by the spectroscopic module 2 passes through the imaging objective lens 500 , the cylindrical lens 510 , the confocal slit 520 and the grating 530 in sequence, and reaches the detection device 540 . The confocal slit 520 is conjugate to the fundus retina 4 plane, and the confocal slit 520 can exclude stray light from non-fundus retina 4 planes from entering the detection device 540, thereby realizing the high resolution of the confocal imaging principle.

The output module 6 is composed of an image acquisition card 600 and an output device 610 , the image acquisition card 600 converts the electrical signal output by the imaging module 5 into an image signal, and outputs it through the output device 610 .

As shown in Figure 5, it is a schematic diagram of the optical path of a specific embodiment of the present invention-a line-scan-based fundus retinal multispectral imaging system and method, and three beams are representatively drawn, all components are arranged along the principal optical axis, and the aperture of the light, etc. High concentricity, the light beams all propagate along the main optical axis of the system. The figures are schematic in nature and do not represent real optical design parameters.

The broadband point light source is a laser light source, or a light emitting diode, or a superluminescent light emitting diode, and a laser light source is used in this embodiment.

The collimating lens is an achromatic lens with a focal length of 50mm.

The cylindrical lens is a common plano-convex cylindrical lens with a focal length of 100mm.

The beam splitter is a broadband beam splitting plate or a broadband beam splitting prism, and a broadband beam splitting prism is used in this embodiment.

The scanning galvanometer is a reflective high-precision scanning galvanometer. In this embodiment, the model 6210H product of CT Company is used, and the effective surface width is 10 mm.

The illumination objective lens group is a beam reduction subsystem composed of two lenses. In this embodiment, two doublet lenses with focal lengths of 100 mm and 50 mm are used to form the beam reduction subsystem.

The imaging objective lens 7 adopts a lens whose focal length is 140mm.

The confocal slit is an adjustable slit. In this embodiment, the APAS80-1A of Beijing Zhuo Li Hanguang is used, and its position is conjugate to the plane of the retina of the fundus.

The grating is a diffraction grating or a holographic grating. In this embodiment, Thorlabs' broadband diffraction grating is used, and the number of line pairs is 300 per millimeter.

The line detector is a linear array charge-coupled device, or a linear array complementary metal oxide semiconductor array, or a linear array photodiode array. In this embodiment, the linear array CCD of e2v company is used, with 1024 line pixels and a pixel size of 14 μm× 14 μm.

The present invention is not limited to the above examples, and those skilled in the art can realize the present invention by adopting various implementation modes according to the content disclosed in the present invention.

Claims (11)

1. the optical fundus retina multi-optical spectrum imaging system based on line sweep comprises Line beam generation module, spectral module, scan module, image-forming module and output module, it is characterized in that:

The Line beam generation module comprises light source, and said light source is single band point light source groups or broadband point source;

Image-forming module comprises sniffer, and said sniffer is line detector group or surface detector.

2. the optical fundus retina multi-optical spectrum imaging system based on line sweep according to claim 1 is characterized in that:

When said light source is the single band point light source groups; The Line beam generation module also comprises fiber coupler, collimating lens and cylindrical lens; The outgoing beam of single band point light source groups is after fiber coupler is coupled into single beam, and single beam is transformed to Line beam through collimating lens collimation and cylindrical lens successively;

When said light source was the broadband point source, the Line beam generation module also comprised collimating lens and cylindrical lens, and the outgoing beam of broadband point source is transformed to Line beam through collimating lens collimation and cylindrical lens successively;

Said Line beam generation module is used for the divergent beams of light source output are generated the one dimension Line beam, and the Line beam generation module links to each other with spectral module.

3. the optical fundus retina multi-optical spectrum imaging system based on line sweep according to claim 1 is characterized in that:

Said spectral module; Be broadband beam split plain film or broadband Amici prism; An one dimension Line beam part that is used for the Line beam generation module is produced directly transmission arrives scan module and will carry out deflection from the imaging beam of scan module reflected back and shines and reach the picture module;

Said scan module; Utilize the Line beam of the direct outgoing of spectral module that human eye optical fundus retina is scanned illumination; With to the imaging beam synchronous reflection of retinal reflex from the optical fundus to spectral module; It is made up of scanning galvanometer and illumination objective lens group; The one dimension Line beam of the direct outgoing of spectral module scans illumination optical fundus retina through said scanning galvanometer and illumination objective lens group by scanning galvanometer successively, and the imaging beam that retinal reflex returns from the optical fundus arrives spectral module through illumination objective lens group and scanning galvanometer synchronous reflection successively;

Said image-forming module; Be used for converting the imaging beam light intensity signal of spectral module deflection outgoing to the signal of telecommunication; And be transferred to output module; It is made up of image-forming objective lens, cylindrical lens, confocal slit, grating and sniffer, and the imaging beam of spectral module deflection outgoing passes through image-forming objective lens, cylindrical lens, grating and confocal slit successively, arrives sniffer;

Said output module is made up of image pick-up card and outut device, and image pick-up card becomes picture signal with the electrical signal conversion of image-forming module output, and through outut device output.

4. the optical fundus retina multi-optical spectrum imaging system based on line sweep according to claim 1 is characterized in that:

Said light source is LASER Light Source or light emitting diode or super-radiance light emitting diode;

When said sniffer was the line detector group, line detector was linear charge-coupled array or linear array complementary metal oxide semiconductors (CMOS) array or linear array photodiode array; When said sniffer was surface detector, surface detector was surface array charge-coupled device or face battle array complementary metal oxide semiconductors (CMOS) array or face battle array photodiode array.

5. according to claim 1,2 or 3 described optical fundus retina multi-optical spectrum imaging systems, it is characterized in that based on line sweep:

Said optical fiber coupler is a N*1 type coupling device, N representative point light sources output beam number;

Said collimating lens is an achromat, and being used for the divergent beams collimation is collimated light beam;

Said cylindrical lens is used for the collimated light beam of collimating lens output is transformed to the one dimension Line beam;

Said scanning galvanometer is the reflecting type high precision scanning galvanometer;

The illumination objective lens group is the bundle subsystem that contracts that two lens are formed;

Said grating is diffraction grating or holographic grating;

Said outut device is a computer.

6. according to claim 1 and 3 described optical fundus retina multi-optical spectrum imaging systems, it is characterized in that: said confocal slit and optical fundus retinal plane conjugation based on line sweep.

7. optical fundus retina multispectral imaging method based on line sweep is characterized in that: comprising:

Step 1, the Line beam generation module generates the single bunch light beam that comprises a plurality of wavelength;

Step 2, the said single bunch light beam that comprises a plurality of wavelength arrives scan module through the direct transmission of a part behind the spectral module;

Step 3, scan module scans illumination through scanning galvanometer and illumination objective lens group to human eye optical fundus retina with the Line beam of the direct outgoing of spectral module, and will be from the optical fundus imaging beam synchronous reflection of retinal reflex to spectral module;

Step 4, spectral module will carry out from the imaging beam of scan module reflected back deflection shine reach the picture module;

Step 5, image-forming module converts the imaging beam light intensity signal of spectral module deflection outgoing to the signal of telecommunication, and is transferred to output module;

Step 6, the image pick-up card in the output module becomes picture signal with the electrical signal conversion of image-forming module output, and through outut device output.

8. the optical fundus retina multispectral imaging method based on line sweep according to claim 7 is characterized in that:

When said light source was the single band point light source groups, said step 1 comprised:

Step 111, a plurality of divergent beams of single band point light source groups outgoing are coupled through fiber coupler, are output as the single beam that includes a plurality of wavelength;

The single beam collimation that step 112, collimating lens will include a plurality of wavelength is a collimated light beam;

Step 113, collimated light beam is transformed to Line beam through cylindrical lens.

When said light source was the broadband point source, said step 1 comprised:

Step 121, the divergent beams of broadband point source outgoing are behind collimating lens, and collimation is a collimated light beam;

Step 122, collimated light beam is transformed to Line beam through cylindrical lens.

9. the optical fundus retina multispectral imaging method based on line sweep according to claim 7, it is characterized in that: said step 5 comprises:

Step 51, image-forming objective lens and cylindrical lens are focused into wire with the imaging beam of spectral module deflection outgoing, arrive grating then;

Step 52, Line beam becomes isolating single wavelength line light beam at different levels through optical grating diffraction;

Step 53, isolating single wavelength line light beams at different levels arrive sniffer, and sniffer converts light intensity signal to the signal of telecommunication.

10. the optical fundus retina multispectral imaging method based on line sweep according to claim 7, it is characterized in that: said step 6 comprises:

Step 61, image pick-up card becomes picture signal with the electrical signal conversion of sniffer output;

Step 62, outut device receive said picture signal, show, handle, and storage is also printed.

11. the optical fundus retina multispectral imaging method based on line sweep according to claim 6 is characterized in that:

Said light source is LASER Light Source or light emitting diode or super-radiance light emitting diode;

When said sniffer was the line detector group, line detector was linear charge-coupled array or linear array complementary metal oxide semiconductors (CMOS) array or linear array photodiode array; When said sniffer was surface detector, surface detector was surface array charge-coupled device or face battle array complementary metal oxide semiconductors (CMOS) array or face battle array photodiode array;

Said optical fiber coupler is a N*1 type coupling device, N representative point light sources output beam number;

Said collimating lens is an achromat, and being used for the divergent beams collimation is collimated light beam;

Said cylindrical lens is used for the collimated light beam of collimating lens output is transformed to the one dimension Line beam;

Said scanning galvanometer is the reflecting type high precision scanning galvanometer;

The illumination objective lens group is the bundle subsystem that contracts that two lens are formed;

Said grating is diffraction grating or holographic grating;

Said outut device is a computer.

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