CN102391034A - Formula and preparation method of Pleurotus eryngii liquid strain culture medium - Google Patents
Formula and preparation method of Pleurotus eryngii liquid strain culture medium Download PDFInfo
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- CN102391034A CN102391034A CN2011100726433A CN201110072643A CN102391034A CN 102391034 A CN102391034 A CN 102391034A CN 2011100726433 A CN2011100726433 A CN 2011100726433A CN 201110072643 A CN201110072643 A CN 201110072643A CN 102391034 A CN102391034 A CN 102391034A
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Abstract
The invention relates to the biological engineering field, in particular to a culture medium and a preparation method thereof. The Pleurotus eryngii liquid strain culture medium comprises raw materials and an aqueous solution. The raw materials comprise the following components by weight percent: 1.5%-2.5% of white granulated sugar, 0.1%-0.3% of soybean meal powder, 0.1%-0.3% of yeast extract, 0.05%-0.1% of potassium dihydrogen phosphate and 0.05%-0.1% of magnesium sulfate. The preparation method of the Pleurotus eryngii liquid strain culture medium comprises the following steps: preparing a culture medium, adjusting, sterilizing and performing inoculation and culture. By adopting the technical scheme, after the product is used as the culture medium of the Pleurotus eryngii liquid strain, the Pleurotus eryngii mycelium has high growth velocity, strong vitality and high mycelium content; and the inoculated strain has high germination rate, short fermentation period and high yield.
Description
Technical field
The present invention relates to bioengineering field, relate in particular to a kind of substratum and preparation method thereof.
Background technology
Pleurotus eryngii has advantages such as bacterial context plumpness, quality be tender and crisp; Particularly its stem dense structure, solid, milky white can all eat, and stem is than the more crisp cunning of cap, tasty and refreshing; Be called as " flat mushroom king ", " dried scallop mushroom "; Mouthfeel with almond flavor and abalone is fit to fresh-keeping, processing, firmly gets liking of people.
Pleurotus eryngii is produced used bacterial classification has solid spawn and liquid spawn, and liquid spawn has advantages such as cost is low, a bacterium is fast, biological transformation ratio height with respect to solid spawn, be the trend of mushroom industry Development Technology innovation.And liquid spawn mainly relies on the nutrient that absorbs liquid nutrient medium to grow.Present existing Pleurotus eryngii liquid spawn culture medium and preparation method exist be prepared into that power is low, mycelial growth is incompatible, the operation professional technique require high, do not possess shortcoming such as versatility, can't popularize use in enormous quantities.
Summary of the invention
The object of the present invention is to provide a kind of Pleurotus eryngii liquid spawn culture medium prescription, to solve the problems of the technologies described above.
Another object of the present invention is to provide a kind of preparation method of Pleurotus eryngii liquid spawn culture medium, to solve the problems of the technologies described above.
The technical problem that the present invention solved can adopt following technical scheme to realize:
Pleurotus eryngii liquid spawn culture medium prescription; Comprise the raw material and the aqueous solution, said raw material comprises following part by weight component: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, sal epsom 0.05%-0.1%.
The present invention adopts the culture medium raw material of said components, and raw material sources are abundant, cheap, and the Pleurotus eryngii mycelial growth that adopts the present invention to turn out is fast, energetic, hyphae content is high, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
Said raw material preferably includes following part by weight component: white sugar 2%, bean cake powder 0.2%, yeast soak powder 0.2%, potassium primary phosphate 0.05%, sal epsom 0.05%, sorghum flour 0.1%-0.3%.
Said raw material also comprises following part by weight component: ginkgo leaf powder leach liquor 0.01%-0.5%.The synthetic collagen protein ingredient content of yellow glucoside, amino acid and amino acid in the Ginkgo Leaf is abundant, helps that Pleurotus eryngii is mycelial to grow.
Said raw material preferably includes following part by weight component: white sugar 1.5%, bean cake powder 0.1%, yeast soak powder 0.1%, sal epsom 0.1%, potassium primary phosphate 0.1%, ginkgo leaf powder leach liquor 0.4%.
The preparation method of Pleurotus eryngii liquid spawn culture medium comprises the steps:
1) substratum configuration: with the white sugar in the raw material, sal epsom, potassium primary phosphate abundant stirring soluble in water, until dissolving;
Bean cake powder in the raw material, yeast are soaked powder add stirring and dissolving in the entry, defoam;
With the above-mentioned aqueous solution that contains white sugar, sal epsom, potassium primary phosphate with contain the aqueous solution mixing that bean cake powder, yeast soak powder, make medium liquid;
2) adjustment: the pH value of adjusting said medium liquid with hydrochloric acid;
3) sterilization: said medium liquid is carried out germicidal treatment in disinfection equipment; Under the situation of not losing nutrition, kill the bacterium in the medium liquid.
4) inoculation culture: the medium liquid after the germicidal treatment is cooled to the 20-25 degree in fermentor tank; Insert the Pleurotus eryngii bacterial classification, regulate culture temperature 20～23 degree, said fermentor tank feeds to purify air and carries out the aerobic cultivation; Fermentation culture 5-8 days, can make the Pleurotus eryngii liquid spawn.
Medium preparation technological operation of the present invention is simple, is applicable to batch production, normalizing operation.
Said raw material comprises following part by weight composition: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, sal epsom 0.05%-0.1%.
Said raw material also comprises ginkgo leaf powder leach liquor 0.01%-0.5%, when the said medium liquid of allotment, adds said ginkgo leaf powder leach liquor, stirs, and contains said ginkgo leaf powder leach liquor in the said medium liquid.
Said raw material preferably includes following part by weight component: white sugar 1.5%, bean cake powder 0.1%, yeast soak powder 0.1%, sal epsom 0.1%, potassium primary phosphate 0.1%, ginkgo leaf powder leach liquor 0.4%.Yeast soaks powder, can adopt yeast extract, as the high-quality nitrogenous source.
During germicidal treatment, adopt the high temperature and high pressure steam sterilization processing mode, said medium liquid is carried out germicidal treatment.
Beneficial effect: owing to adopt technique scheme, behind the substratum of the present invention as the Pleurotus eryngii liquid spawn, the Pleurotus eryngii mycelial growth is fast, energetic, hyphae content is high, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
Description of drawings
Fig. 1 is preparing method's of the present invention schema.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach purpose and effect and be easy to understand and understand, further set forth the present invention below in conjunction with concrete diagram.
Pleurotus eryngii liquid spawn culture medium prescription; Comprise the raw material and the aqueous solution;, raw material comprises following part by weight component: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, sal epsom 0.05%-0.1%.Raw material preferably includes following part by weight component: white sugar 2%, bean cake powder 0.2%, yeast soak powder 0.2%, potassium primary phosphate 0.08%, sal epsom 0.07%, sorghum flour 0.1%-0.3%.The present invention adopts the culture medium raw material of said components, and raw material sources are abundant, cheap, and the Pleurotus eryngii mycelial growth that adopts the present invention to turn out is fast, energetic, hyphae content is high, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
Raw material also comprises following part by weight component: ginkgo leaf powder leach liquor 0.01%-0.5%.The synthetic collagen protein ingredient content of yellow glucoside, amino acid and amino acid in the Ginkgo Leaf is abundant, helps that Pleurotus eryngii is mycelial to grow.Raw material preferably includes following part by weight component: white sugar 1.5%, bean cake powder 0.1%, yeast soak powder 0.1%, sal epsom 0.1%, potassium primary phosphate 0.1%, ginkgo leaf powder leach liquor 0.4%.
Bean cake powder provides nitrogen nutriment as imitating nitrogenous source late.Yeast soaks powder, as quick-acting nitrogenous sources, nitrogen nutriment is provided fast.Helping Pleurotus eryngii grows up fast.And can effectively guarantee the growth quality of mycelia.Help the mycelia balling-up, and the balling-up shape intact, help the growing state of visual determination mycelia.
With reference to Fig. 1, the preparation method of Pleurotus eryngii liquid spawn culture medium comprises the steps: the first step, substratum configuration: with the white sugar in the raw material, sal epsom, potassium primary phosphate abundant stirring soluble in water, until dissolving.Bean cake powder in the raw material, yeast are soaked powder add stirring and dissolving in the entry, defoam.With the above-mentioned aqueous solution that contains white sugar, sal epsom, potassium primary phosphate with contain the aqueous solution mixing that bean cake powder, yeast soak powder, make medium liquid.Raw material when substratum disposes in the first step is allocated according to following part by weight composition: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, sal epsom 0.05%-0.1%.Raw material also comprises ginkgo leaf powder leach liquor 0.01%-0.5%, when the allotment medium liquid, adds the ginkgo leaf powder leach liquor, stirs, and contains the ginkgo leaf powder leach liquor in the medium liquid.
Second step, adjustment: with the pH value of hydrochloric acid adjustment medium liquid;
The 3rd step, sterilization: medium liquid is carried out germicidal treatment in disinfection equipment; Under the situation of not losing nutrition, kill the bacterium in the medium liquid.During germicidal treatment, adopt the high-temperature sterilization treatment mode, medium liquid is carried out germicidal treatment.
The 4th step, inoculation culture: the medium liquid after the germicidal treatment is cooled in fermentor tank below the 20-25 degree, inserts the Pleurotus eryngii bacterial classification; Regulate culture temperature 20～23 degree; Said fermentor tank feeds to purify air and carries out the aerobic cultivation, fermentation culture 5-8 days, can make the Pleurotus eryngii liquid spawn.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; The present invention is not restricted to the described embodiments; That describes in the foregoing description and the specification sheets just explains principle of the present invention; Under the prerequisite that does not break away from spirit and scope of the invention, the present invention also has various changes and modifications, and these variations and improvement all fall in the scope of the invention that requires protection.
The present invention requires protection domain to be defined by appending claims and equivalent thereof.
Claims (8)
1. the Pleurotus eryngii liquid spawn culture medium is filled a prescription; Comprise the raw material and the aqueous solution; It is characterized in that said raw material comprises following part by weight component: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, sal epsom 0.05%-0.1%.
2. Pleurotus eryngii liquid spawn culture medium prescription according to claim 1, it is characterized in that: said raw material comprises following part by weight component: white sugar 2%, bean cake powder 0.2%, yeast soak powder 0.2%, sal epsom 0.05%, potassium primary phosphate 0.05%, sorghum flour 0.1%-0.3%.
3. Pleurotus eryngii liquid spawn culture medium prescription according to claim 1, it is characterized in that: said raw material also comprises following part by weight component: ginkgo leaf powder leach liquor 0.01%-0.5%.
4. Pleurotus eryngii liquid spawn culture medium prescription according to claim 3, it is characterized in that: said raw material comprises following part by weight component: white sugar 1.5%, bean cake powder 0.1%, yeast soak powder 0.1%, sal epsom 0.1%, potassium primary phosphate 0.1%, ginkgo leaf powder leach liquor 0.4%.
5. the preparation method of Pleurotus eryngii liquid spawn culture medium is characterized in that, comprises the steps:
1) substratum configuration: with the white sugar in the raw material, sal epsom, potassium primary phosphate abundant stirring soluble in water, until dissolving;
Bean cake powder in the raw material, yeast are soaked powder add stirring and dissolving in the entry, defoam;
With the above-mentioned aqueous solution that contains white sugar, sal epsom, potassium primary phosphate with contain the aqueous solution mixing that bean cake powder, yeast soak powder, make medium liquid;
2) adjustment: the pH value of adjusting said medium liquid with hydrochloric acid;
3) sterilization: said medium liquid is carried out germicidal treatment in disinfection equipment;
4) inoculation culture: the medium liquid after the germicidal treatment is cooled in fermentor tank below the 20-25 degree; Insert the Pleurotus eryngii bacterial classification, regulate culture temperature 20～23 degree, said fermentor tank feeds to purify air and carries out the aerobic cultivation; Fermentation culture 5-8 days, can make the Pleurotus eryngii liquid spawn.
6. the preparation method of Pleurotus eryngii liquid spawn culture medium according to claim 5; It is characterized in that said raw material comprises following part by weight composition: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, sal epsom 0.05%-0.1%.
7. the preparation method of Pleurotus eryngii liquid spawn culture medium according to claim 5; It is characterized in that; Said raw material also comprises ginkgo leaf powder leach liquor 0.01%-0.5%; When the said medium liquid of allotment, add said ginkgo leaf powder leach liquor, stir, contain said ginkgo leaf powder leach liquor in the said medium liquid.
8. the preparation method of Pleurotus eryngii liquid spawn culture medium according to claim 6 is characterized in that, during germicidal treatment, the mode that adopts high temperature and ultra-violet sterilization to combine is carried out germicidal treatment to said medium liquid.
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Cited By (12)
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|---|---|---|---|---|
| CN102845219A (en) * | 2012-09-06 | 2013-01-02 | 西北农林科技大学 | Cultivation technique of Pleurotus eryngii |
| CN102964156A (en) * | 2012-08-30 | 2013-03-13 | 上海雪榕生物科技股份有限公司 | Culture medium formula and preparation method of Pleurotus nebrodensis liquid spawn |
| CN102976852A (en) * | 2012-12-12 | 2013-03-20 | 天水众兴菌业科技股份有限公司 | Water-soluble culture medium for edible fungus liquefaction strain |
| CN102972208A (en) * | 2012-12-12 | 2013-03-20 | 天水众兴菌业科技股份有限公司 | Method for preparing edible mushroom liquefied strains |
| CN103242080A (en) * | 2013-05-20 | 2013-08-14 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
| CN103503695A (en) * | 2013-10-14 | 2014-01-15 | 上海市农业科学院 | Method for cultivating pleurotus eryngii liquid strains |
| CN103508809A (en) * | 2013-10-14 | 2014-01-15 | 上海市农业科学院 | Pleurotus eryngii liquid strain culture medium and preparation method thereof |
| CN104145714A (en) * | 2014-07-31 | 2014-11-19 | 陆良爨乡绿园菇业有限公司 | Effective breeding method for king trumpet mushroom production |
| CN105238699A (en) * | 2015-09-16 | 2016-01-13 | 江苏华绿生物科技股份有限公司 | Preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit |
| CN107750823A (en) * | 2017-11-28 | 2018-03-06 | 申国庆 | The culture medium of pleurotus eryngii liquid fermentation |
| CN108812066A (en) * | 2018-07-06 | 2018-11-16 | 山东省科创食用菌产业技术研究院 | A kind of liquid spawn culture medium formula and technique improving Pleurotus eryngii content of glutamic acid |
| CN109588213A (en) * | 2018-12-12 | 2019-04-09 | 湖北省农业科学院经济作物研究所 | A kind of pleurotus eryngii liquid strain fast preparation method |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102964156A (en) * | 2012-08-30 | 2013-03-13 | 上海雪榕生物科技股份有限公司 | Culture medium formula and preparation method of Pleurotus nebrodensis liquid spawn |
| CN102845219A (en) * | 2012-09-06 | 2013-01-02 | 西北农林科技大学 | Cultivation technique of Pleurotus eryngii |
| CN102845219B (en) * | 2012-09-06 | 2013-11-06 | 西北农林科技大学 | Cultivation technique of Pleurotus eryngii |
| CN102976852A (en) * | 2012-12-12 | 2013-03-20 | 天水众兴菌业科技股份有限公司 | Water-soluble culture medium for edible fungus liquefaction strain |
| CN102972208A (en) * | 2012-12-12 | 2013-03-20 | 天水众兴菌业科技股份有限公司 | Method for preparing edible mushroom liquefied strains |
| CN102972208B (en) * | 2012-12-12 | 2015-01-07 | 天水众兴菌业科技股份有限公司 | Method for preparing edible mushroom liquefied strains |
| CN103242080B (en) * | 2013-05-20 | 2014-08-13 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
| CN103242080A (en) * | 2013-05-20 | 2013-08-14 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
| CN103508809A (en) * | 2013-10-14 | 2014-01-15 | 上海市农业科学院 | Pleurotus eryngii liquid strain culture medium and preparation method thereof |
| CN103503695A (en) * | 2013-10-14 | 2014-01-15 | 上海市农业科学院 | Method for cultivating pleurotus eryngii liquid strains |
| CN104145714A (en) * | 2014-07-31 | 2014-11-19 | 陆良爨乡绿园菇业有限公司 | Effective breeding method for king trumpet mushroom production |
| CN105238699A (en) * | 2015-09-16 | 2016-01-13 | 江苏华绿生物科技股份有限公司 | Preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit |
| CN107750823A (en) * | 2017-11-28 | 2018-03-06 | 申国庆 | The culture medium of pleurotus eryngii liquid fermentation |
| CN108812066A (en) * | 2018-07-06 | 2018-11-16 | 山东省科创食用菌产业技术研究院 | A kind of liquid spawn culture medium formula and technique improving Pleurotus eryngii content of glutamic acid |
| CN109588213A (en) * | 2018-12-12 | 2019-04-09 | 湖北省农业科学院经济作物研究所 | A kind of pleurotus eryngii liquid strain fast preparation method |
| CN109588213B (en) * | 2018-12-12 | 2021-02-26 | 湖北省农业科学院经济作物研究所 | Rapid preparation method of pleurotus eryngii liquid strain |
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Application publication date: 20120328 |