CN102381896B - Crab-flavor mushroom liquid strain medium formula and preparation method thereof - Google Patents
Crab-flavor mushroom liquid strain medium formula and preparation method thereof Download PDFInfo
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- CN102381896B CN102381896B CN201110072837.3A CN201110072837A CN102381896B CN 102381896 B CN102381896 B CN 102381896B CN 201110072837 A CN201110072837 A CN 201110072837A CN 102381896 B CN102381896 B CN 102381896B
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- 239000007788 liquid Substances 0.000 title claims abstract description 48
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 40
- 239000000796 flavoring agent Substances 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 238000009472 formulation Methods 0.000 title description 2
- 239000000843 powder Substances 0.000 claims abstract description 66
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 34
- 239000002994 raw material Substances 0.000 claims abstract description 33
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 18
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 18
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 17
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 17
- 239000007864 aqueous solution Substances 0.000 claims abstract description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 10
- 238000000855 fermentation Methods 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 18
- 229910019142 PO4 Inorganic materials 0.000 claims description 16
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 16
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 16
- 229930006000 Sucrose Natural products 0.000 claims description 16
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 16
- 239000010452 phosphate Substances 0.000 claims description 16
- 239000011591 potassium Substances 0.000 claims description 16
- 229910052700 potassium Inorganic materials 0.000 claims description 16
- 210000000582 semen Anatomy 0.000 claims description 16
- 241000218628 Ginkgo Species 0.000 claims description 14
- 235000011201 Ginkgo Nutrition 0.000 claims description 14
- 235000008100 Ginkgo biloba Nutrition 0.000 claims description 14
- 230000002070 germicidal effect Effects 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 4
- 240000006394 Sorghum bicolor Species 0.000 claims description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 230000035784 germination Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 229940041514 candida albicans extract Drugs 0.000 abstract description 2
- 239000012138 yeast extract Substances 0.000 abstract description 2
- 235000021552 granulated sugar Nutrition 0.000 abstract 1
- 238000011177 media preparation Methods 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 25
- 150000001413 amino acids Chemical class 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
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- 108010035532 Collagen Proteins 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 229930182478 glucoside Natural products 0.000 description 2
- 150000008131 glucosides Chemical class 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
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Abstract
The invention relates to the field of bioengineering, and in particular relates to a medium and a preparation method thereof. A crab-flavor mushroom liquid strain medium formula comprises raw materials and an aqueous solution, wherein the raw materials comprise the following components by weight percent: 1.5%-2.5% of white granulated sugar, 0.1%-0.3% of bean pulp powder, 0.1%-0.3% of yeast extract powder, 0.05%-0.1% of monopotassium phosphate and 0.05%-0.1% of magnesium sulfate. The preparation method of the crab-flavor mushroom liquid strain medium formula comprises the following steps of medium preparation, regulation, sterilization and inoculating culture. Because the technical scheme is utilized, after the medium of the invention is used as the medium of crab-flavor mushroom liquid strains, the crab-flavor mushroom mycelium is rapid in growth, strong in activity and high in mycelium content; and the inoculated strain is good in germination rate, short in fermentation period and high in yield.
Description
Technical field
The present invention relates to bioengineering field, relate in particular to a kind of substratum and preparation method thereof.
Background technology
Crab mushroom has the advantages such as taste is fresher than flat mushroom, meat is thicker than sliding mushroom, matter is more tough than mushroom, excellent taste, in crab mushroom, contain and enrich VITAMIN and 17 seed amino acids, wherein Methionin, arginic content, higher than general mushroom class, contribute to teenager's intelligence development to increase, anticancer, reduction cholesterol.Crab mushroom also has unique crab fragrance, is one of world-renowned edible mushrooms.Crab mushroom is produced bacterial classification used solid spawn and liquid spawn, and liquid spawn has the advantages such as cost is low, a bacterium is fast, biological transformation ratio is high with respect to solid spawn, is the trend of Edible Fungi Industry Development technical renovation.And liquid spawn mainly relies on the nutrient that absorbs liquid nutrient medium to grow.Current existing crab-flavor mushroom liquid strain medium and preparation method exist and are prepared into that power is low, mycelial growth inadaptable, operation professional technique require high, do not possess the shortcomings such as versatility, cannot popularize in enormous quantities use.
Summary of the invention
The object of the present invention is to provide a kind of crab-flavor mushroom liquid strain medium formula, to solve the problems of the technologies described above.
Another object of the present invention is to provide a kind of preparation method of crab-flavor mushroom liquid strain medium, to solve the problems of the technologies described above.
Technical problem solved by the invention can realize by the following technical solutions:
Crab-flavor mushroom liquid strain medium formula, comprise raw material and the aqueous solution, described raw material comprises following part by weight component: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%.
Described raw material also comprises Semen Maydis powder 0.2%-0.4%.
Described raw material preferably includes following part by weight component: white sugar 2%, bean cake powder 0.2%, Semen Maydis powder 0.3%, yeast soak powder 0.2%, potassium primary phosphate 0.07%, magnesium sulfate 0.08%, sorghum flour 0.1%-0.3%.
The present invention adopts the culture medium raw material of said components, and raw material sources are abundant, cheap, and the crab mushroom mycelial growth that adopts the present invention to turn out is fast, energetic, hyphae content is high, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
Described raw material also comprises following part by weight component: ginkgo leaf powder leach liquor 0.01%-0.5%.The synthetic collagen protein ingredient rich content of yellow glucoside, amino acid and amino acid in Ginkgo Leaf, contributes to growing of yellow golden mushroom mycelium.
The preparation method of crab-flavor mushroom liquid strain medium, comprises the steps:
1) substratum configuration: by the white sugar in raw material, magnesium sulfate, potassium primary phosphate abundant stirring soluble in water, until dissolve;
Bean cake powder in raw material, Semen Maydis powder, yeast are soaked to powder and be added to the water stirring and dissolving, defoam;
The above-mentioned aqueous solution that contains white sugar, magnesium sulfate, potassium primary phosphate and the aqueous solution that contains bean cake powder, Semen Maydis powder, yeast and soak powder are mixed, make medium liquid;
2) adjust: the pH value of adjusting described medium liquid with hydrochloric acid;
3) sterilization: described medium liquid is carried out in disinfection equipment to germicidal treatment; In the situation that not losing nutrition, kill the bacterium in medium liquid.
4) inoculation culture: the medium liquid after germicidal treatment is cooled to below 25 degree in fermentor tank, access crab mushroom bacterial classification, regulates culture temperature 18～20 degree, and described fermentor tank passes into purify air and carries out aerobic cultivation, fermentation culture 5-8 days, can make crab mushroom liquid spawn.
Substratum preparation technology of the present invention is simple to operate, is applicable to batch production, normalizing operation.
Described raw material comprises following part by weight composition: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, Semen Maydis powder 0.2%-0.4%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%.
Described raw material also comprises ginkgo leaf powder leach liquor 0.01%-0.5%, when the described medium liquid of allotment, adds described ginkgo leaf powder leach liquor, stirs, and contains described ginkgo leaf powder leach liquor in described medium liquid.Yeast soaks powder, can adopt yeast extract, as high-quality nitrogenous source.
During germicidal treatment, adopt high temperature and high pressure steam sterilization processing mode, described medium liquid is carried out to germicidal treatment.
Beneficial effect: owing to adopting technique scheme, after the substratum of crab mushroom liquid spawn, crab mushroom mycelial growth is fast, energetic, hyphae content is high using the present invention, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
Accompanying drawing explanation
Fig. 1 is preparation method's of the present invention schema.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with concrete diagram, further set forth the present invention.
Crab-flavor mushroom liquid strain medium formula, comprise raw material and the aqueous solution, raw material comprises following part by weight component: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%.Raw material also comprises Semen Maydis powder 0.2%-0.4%.Raw material preferably includes following part by weight component: white sugar 2%, bean cake powder 0.2%, Semen Maydis powder 0.3%, yeast soak powder 0.2%, potassium primary phosphate 0.07%, magnesium sulfate 0.08%.The present invention adopts the culture medium raw material of said components, and raw material sources are abundant, cheap, and the crab mushroom mycelial growth that adopts the present invention to turn out is fast, energetic, hyphae content is high, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
Raw material also comprises following part by weight component: ginkgo leaf powder leach liquor 0.01%-0.5%.The synthetic collagen protein ingredient rich content of yellow glucoside, amino acid and amino acid in Ginkgo Leaf, contributes to growing of yellow golden mushroom mycelium.Raw material preferably includes following part by weight component: white sugar 1.5%, bean cake powder 0.1%, Semen Maydis powder 0.2%, yeast soak powder 0.2%, potassium primary phosphate 0.09%, magnesium sulfate 0.09%, straw powder 1%, ginkgo leaf powder leach liquor 0.4%, sorghum flour 0.1%-0.3%.
Bean cake powder, as slow effect nitrogenous source, provides nitrogen nutriment.Yeast soaks powder, and as quick-acting nitrogenous sources, Quick is for nitrogen nutriment.Be conducive to crab mushroom Fast Growth.And can effectively guarantee the growth quality of mycelia.Be conducive to mycelia balling-up, and balling-up shape is complete, is conducive to the growing state of visual determination mycelia.
With reference to Fig. 1, the preparation method of crab-flavor mushroom liquid strain medium, comprises the steps: the first step, substratum configuration: by the white sugar in raw material, magnesium sulfate, potassium primary phosphate abundant stirring soluble in water, until dissolve.Bean cake powder in raw material, Semen Maydis powder, yeast are soaked to powder and be added to the water stirring and dissolving, defoam.The above-mentioned aqueous solution that contains white sugar, magnesium sulfate, potassium primary phosphate and the aqueous solution that contains bean cake powder, Semen Maydis powder, yeast and soak powder are mixed, make medium liquid.Raw material when substratum configures in the first step is allocated according to following part by weight composition: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, Semen Maydis powder 0.2%-0.4%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%.
Raw material also comprises ginkgo leaf powder leach liquor 0.01%-0.5%, when allotment medium liquid, adds ginkgo leaf powder leach liquor, stirs, and contains ginkgo leaf powder leach liquor in medium liquid.
Second step, adjusts: the pH value of adjusting medium liquid with hydrochloric acid;
The 3rd step, sterilization: medium liquid is carried out in disinfection equipment to germicidal treatment; In the situation that not losing nutrition, kill the bacterium in medium liquid.During germicidal treatment, adopt high temperature and high pressure steam sterilization processing mode, medium liquid is carried out to germicidal treatment.
The 4th step, inoculation culture: the medium liquid after germicidal treatment is cooled to below 25 degree in fermentor tank to access crab mushroom bacterial classification, regulate culture temperature 18～20 degree, fermentor tank passes into purify air and carries out aerobic cultivation, and fermentation culture 5-8 days, can make crab mushroom liquid spawn.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (5)
1. crab-flavor mushroom liquid strain medium, comprise raw material and the aqueous solution, it is characterized in that, described raw material comprises following part by weight component: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%, ginkgo leaf powder leach liquor 0.01%-0.5%.
2. crab-flavor mushroom liquid strain medium according to claim 1, is characterized in that: described raw material also comprises Semen Maydis powder 0.2%-0.4%.
3. crab-flavor mushroom liquid strain medium according to claim 2, is characterized in that: described raw material comprises following part by weight component: white sugar 2%, bean cake powder 0.2%, Semen Maydis powder 0.3%, yeast soak powder 0.2%, potassium primary phosphate 0.07%, magnesium sulfate 0.08%, sorghum flour 0.1%-0.3%.
4. the preparation method of crab mushroom liquid spawn, is characterized in that, comprises the steps:
1) substratum configuration: by the white sugar in raw material, magnesium sulfate, potassium primary phosphate abundant stirring soluble in water, until dissolve;
Bean cake powder in raw material, Semen Maydis powder, yeast are soaked to powder and be added to the water stirring and dissolving, defoam;
The above-mentioned aqueous solution that contains white sugar, magnesium sulfate, potassium primary phosphate and the aqueous solution that contains bean cake powder, Semen Maydis powder, yeast and soak powder are mixed, make medium liquid;
2) adjust: the pH value of adjusting described medium liquid with hydrochloric acid;
3) sterilization: described medium liquid is carried out in disinfection equipment to germicidal treatment; In the situation that not losing nutrition, kill the bacterium in medium liquid;
4) inoculation culture: the medium liquid after germicidal treatment is cooled to below 25 degree in fermentor tank, access crab mushroom bacterial classification, regulates culture temperature 18～20 degree, and described fermentor tank passes into purify air and carries out aerobic cultivation, fermentation culture 5-8 days, can make crab mushroom liquid spawn;
Described raw material comprises following part by weight composition: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, Semen Maydis powder 0.2%-0.4%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%;
Described raw material also comprises ginkgo leaf powder leach liquor 0.01%-0.5%, when the described medium liquid of allotment, adds described ginkgo leaf powder leach liquor, stirs.
5. the preparation method of crab mushroom liquid spawn according to claim 4, is characterized in that, during germicidal treatment, adopts high temperature and high pressure steam sterilization processing mode, and described medium liquid is carried out to germicidal treatment.
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| CN102964152A (en) * | 2012-08-30 | 2013-03-13 | 上海雪榕生物科技股份有限公司 | Culture medium formula and preparation method of white beech mushroom liquid spawn |
| CN102976852A (en) * | 2012-12-12 | 2013-03-20 | 天水众兴菌业科技股份有限公司 | Water-soluble culture medium for edible fungus liquefaction strain |
| CN102972208B (en) * | 2012-12-12 | 2015-01-07 | 天水众兴菌业科技股份有限公司 | Method for preparing edible mushroom liquefied strains |
| CN105837324B (en) * | 2016-03-28 | 2017-04-12 | 东莞市合心生物科技有限公司 | Hypsizigus marmoreus liquid strain medium and planting method |
| CN106047720A (en) * | 2016-06-27 | 2016-10-26 | 合肥福泉现代农业科技有限公司 | Red-date-peel-powder-based Hypsizygus marmoreus culture medium and Hypsizygus marmoreus liquid strain preparation method |
| CN112840962B (en) * | 2021-04-14 | 2022-08-23 | 江苏海洋大学 | Preparation method of Hypsizygus marmoreus liquid strain |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101293791A (en) * | 2008-06-13 | 2008-10-29 | 上海浦东天厨菇业有限公司 | Liquid bacterial culture medium for industrial preparation of hypsizygus marmoreus and preparation method thereof |
| CN101317527A (en) * | 2007-06-08 | 2008-12-10 | 上海丰科生物科技股份有限公司 | Process for preparing protospecies of white beech mushroom |
| CN101743843A (en) * | 2008-12-18 | 2010-06-23 | 上海丰科生物科技股份有限公司 | Method for cultivating brown crab taste mushroom |
| CN101891515A (en) * | 2009-05-18 | 2010-11-24 | 李畅 | Hypsizigus marmoreus compost |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101317527A (en) * | 2007-06-08 | 2008-12-10 | 上海丰科生物科技股份有限公司 | Process for preparing protospecies of white beech mushroom |
| CN101293791A (en) * | 2008-06-13 | 2008-10-29 | 上海浦东天厨菇业有限公司 | Liquid bacterial culture medium for industrial preparation of hypsizygus marmoreus and preparation method thereof |
| CN101743843A (en) * | 2008-12-18 | 2010-06-23 | 上海丰科生物科技股份有限公司 | Method for cultivating brown crab taste mushroom |
| CN101891515A (en) * | 2009-05-18 | 2010-11-24 | 李畅 | Hypsizigus marmoreus compost |
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