CN102350007B - Preparation method of human-body-absorbable medical face-lifting product - Google Patents
Preparation method of human-body-absorbable medical face-lifting product Download PDFInfo
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- CN102350007B CN102350007B CN201110315443.6A CN201110315443A CN102350007B CN 102350007 B CN102350007 B CN 102350007B CN 201110315443 A CN201110315443 A CN 201110315443A CN 102350007 B CN102350007 B CN 102350007B
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Transplantation (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
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- Urology & Nephrology (AREA)
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention relates to a preparation method of a human-body-absorbable medical face-lifting product. The invention discloses a low-carbon and environment-friendly face-lifting product. Muscles of a tail and foot muscles of four limbs of a nutria are utilized, are neural fibrous tendons formed by all-natural collagens, are subjected to enzymolysis through a biological protease, and are made into various types of platy, strip and liquid products. The human-body-absorbable medical face-lifting product has no exclusion, can be absorbed by a human body, further has no toxic or side effects, and is used for the face lifting and the beautification of the human body, the skin grafting for the injury, the burn and the scald of the epithelium of the human body, the complementary grafting for the injuries of the ear drum membrane and the like of the human body, the complementary grafting for the injury of the membrane vavle of visceral organs of the human body and the defective repair for the wounds of the muscles and the tendons. The human-body-absorbable medical face-lifting product can play a role of a cartilage framework for the rhinoplasty face lifting of the human body, and has quick implantation wound concrescence and pain-free symptom for a minimally invasive endoscopic technique implemented for breast augmentation. All breast-augmenting products which cannot be absorbed by the human body on the market at present can be replaced, and the human-body-absorbable medical face-lifting product is a face-lifting product which can be absorbed by the human body, is an absorbable natural collagen made of a natural raw material, and is an ideal medical face-lifting product, which can be called the 'welding rods' of the human body for short, is 'welded' at positions where defects exist, and can be absorbed by the human body.
Description
Technical field
The present invention relates to a kind of medical face-lifting, beautification product preparation method, to the disappearance of human body skin, the repairing etc. of the defect of tendon, augmentation rhinoplasty, breast enlargement, the rough cicatrix of reparation human body surface skin, substitutes the preparation method of true soma goods.
Background technology
Nutria, also known as coypu, English name: Nutria, Russia's name: Hytnr, is the dualpurpose large-scale rodent of a kind of skin.Its two hind leg toe has web can live in water, and body weight is about about 5-15 kilogram; Its leathercraft is first-class delicacies by the favor of consumers in general, fine and tender taste, delicious flavour.Its oils and fats is the medicinal raw material of surgery various diseases, is also the high-grade materials producing advanced skin care product.The tail of adult nutria is sturdy, coning, about 30 centimeters long to 40 centimeters, size is had in tail, thickness, tail muscle different in size, also be one of Main Ingredients and Appearance of nutria tail, this tail muscle pulling force is strong, the pulling force of every root muscle is more than 10-15 kilogram, lamellar or strip goods that diameter is 0.1 millimeter to 2 millimeters can be made into, the four-leg muscle of nutria equally can film-making, this tail muscle and foot muscle do not reject, can be absorbed by when injected organism tissue, thus biofermentation enzymolysis process is utilized to make human facelift, beautification product, substitute the medical product of the true soma of human body.
The tail muscle of nutria is also widely used in clinical as suture, the accurate font size products of traditional Chinese medicines, it is the collagen sutures of pure natural, it is better than all kinds of surgical sutures used clinically now, it does not reject, people's physical ability is absorbed in not to be needed to remove line clinically, and wound shifts to an earlier date healing in 3 days than like product, preventing from scar.Thus, utilize the tail muscle of nutria and the feature of foot muscle does not reject, pulling force is strong, soft, good air permeability after processing, the feature that can be absorbed by when injected organism tissue, the goods it being made different size replace tissue to be used for human body epithelium, substitute the augmentation rhinoplasty of human body true soma, breast enlargement, reparation breakdown tendon for the skin-grafting of burning, scalding and human facelift, repair the excellent goods of damaged tympanum etc.
Summary of the invention
The present invention produces product by " fermentation technology " bio protease enzyme solution, peeling routinely after nutria is slaughtered, then with sharp weapon by tail after the method process of cutting tail in the root of the tail portion of nutria according to extraction nutria tail muscle, tail muscle is extracted with cramp machine, put into fermentation tank, add bio protease simultaneously, the amount adding bio protease is that 150 units/gram to 1200 units/gram of the gross weight of tail muscle and four-leg muscle enter fermentation tank, carry out heat-preservation fermentation, fermentation temperature controls between 25 DEG C to 45 DEG C; Constant temperature keeps after fermentation in 1-72 hour to terminate, and takes out tail muscle and four-leg muscle, puts into the goods that special mould machine makes lamellar, strip, the various shape such as liquid, all size after taking off ferment treatment respectively.
Present invention also offers a kind of human-body-absorbable medical face-lifting product preparation method, utilize nutria tail muscle by under the zymolysis of bio protease, be convenient to all types of skin-grafting epithelium that molding can manufacture needed by human body, repair Tendon Defection, the lamellar of augmentation rhinoplasty or breast enlargement, the face-lifting goods of strip or liquid state, it is characterized in that: nutria tail muscle is sent in enzymatic vessel, add bio protease and carry out enzymolysis, enzyme concentration is 150 units/gram to 1200 Dan Wei ∕ gram, assist enzymolysis must add 0.001 gram/gram, calcium chloride, must add sodium fluoride 0.002 gram/gram to 0.02 gram/gram adjusts pH value to be 4-7.
When fermentation tank carries out enzymolysis, enzymolysis constant temperature is at 25 DEG C-45 DEG C, and enzymolysis time is 3-72 hour.
Add Sal 10-15% in time in the enzymolysis later stage, change the electrolyte balance of tail muscle, promote tail muscle inside to unite structural degradation, decompose tail muscle further; Then tail muscle is entered emulsifying in mortar machine, through emulsifying tail muscle fibre diameter at 0.0001-0.1 millimeter.
When emulsifying, the nutria oils and fats preferably adding 5% participates in the emulsifying of tail muscle, can be the liquid product of less than 0.001 millimeter to diameter by the emulsifying of tail muscle.
In preparation method of the present invention, bio protease is selected from the one in aspergillus terricola 3942 protease, bacillus subtilis 1398 protease and bacillus subtilis S114 protease.
Tool of the present invention has the following advantages: low-carbon environment-friendly processing technology is simple, production process three-waste free discharge, free from environmental pollution, product is made up of the biological collagen of pure natural, do not reject, to any when injected organism tissue can in conjunction with, fused, resistance infection is strong, wound healing is fast, without any side effects to human body.Need not hide protection by unclassified stores, its energy self-protection wound surface, the goods that permeability is good, absorption is good, good water-retaining property, permeability are strong, play the human body of true soma for human facelift, beauty treatment, the breakage of reparation human body, the succedaneum of true soma.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further elaborated
Embodiment one:
1, the making of medical epithelium: after nutria is slaughtered, by upper rule layer stripping, peel off skin after extract tail from nutria tail root, send into sterilizing room, after cramp PROCESS FOR TREATMENT, extract tail muscle with cramp machine.
2, sent in enzymatic vessel by 100 grams, the tail muscle extracted, add bio protease 3942, enzyme concentration is 500 units/gram, and adjust pH value to be 6, carry out constant temperature 35 DEG C of enzymolysis, the time is 12 hours.
3, add Sal 15% after 12 hours through enzymolysis tail muscle to carry out " expanded ", because the electrolyte balance changed in tail muscle that adds of Sal promotes that the structural degradation of uniting of tail muscle inside decomposes tail muscle fiber further, also be " a biological puffing process ", salt adding continues enzymolysis again and takes out tail muscle after 12 hours to take off enzyme desalination stand-by.
4, the tail muscle after de-enzyme, desalination is put in mortar machine and is carried out emulsifying, and through microscopy tail muscle fibre diameter in 0.001 millimeter, length is not limited to liquid semi-finished product.
5, manufacture mould of skin film, make with corrosion resistant plate, place framework, the high 0.5 millimeter of square of framework surrounding, frame inner area 100 square centimeters that rustless steel is made on stainless steel.
6, at this moment put into by semi-finished product in mould frame, carrying out rolling pushing, be solid blank with roller film laminator on film, finished product is through airing sterilizing, and goods reduced, to 0.1 millimeter of natural shrinking 4/5, is finished product.
7, as needed the goods of different size, need only zoom in or out frame distance, increasing frame or reducing frame height and operate, be the finished product of different size.
Embodiment two
1, the making of medical human tendon goods: by extracting tail muscle method in embodiment one, extracting 100 grams, tail muscle is being 1 wisp colligation with every 10 muscle, put into enzymatic vessel, add bio protease 1.398,150 units/gram, add 0.001 gram/gram, calcium chloride again, constant temperature 36 DEG C of enzymolysis times are 6 hours, and taking out through taking off enzyme is semi-finished product.
2, manufacture tendon goods mould corrosion resistant plate and make that mold is convex, bed die is Baltimore groove, groove depth 5 millimeters, groove width 2 millimeters, length is not limit, patrix is convex, convexly rises 4 millimeters, and patrix convex top contract enters side seam in lower geosynclinal concave groove and is no more than 0.01 millimeter, is shaping dies.
3, semi-finished product being put into bed die groove sets level whole, and the upper and lower concavo-convex docking of the mold that closes 1 kilogram of exert pressure, be finished product through drying, finished diameter is 1 millimeter.Wide is 2 millimeters, and length is not limit.
4, as needed the goods of different size, the specification on the convex top of groove of mould specification should be changed, size is produced is finished product.
Embodiment three
1, the making of medical breast enlargement goods: extract 100 grams, tail muscle and put into enzymatic vessel by extracting tail muscle method in embodiment one and add bio protease 3942 again, enzyme concentration is 300 units/gram, add 0.001 gram/gram, calcium chloride again, add sodium fluoride 0.002 gram/gram again, constant temperature 35 DEG C of enzymolysis 6 hours de-enzymes are solid blank.
2, the Nutrias fat 5% that the tail muscle after de-enzyme adds through low-temperature refining enters mortar machine and the together emulsifying of tail muscle, randomization, and detect tail muscle fiber is liquid semi-finished product below 0.001 millimeter.Liquid semi-finished product, through process such as sterilizings, are implanted as liquid finished product for Wicresoft's endoscopic technique.
3, preparing medical breast enlargement goods, palpus stainless steel mould processed, arranges framework, high 5 millimeters of " Banana Type " frame inner areas 20 square centimeters in framework surrounding on stainless steel.
4, at this moment put into by semi-finished product in punching block frame, being 0.2 kilogram with drum-type film laminator pressure, to carry out pushing airing be finished product, and finished diameter is 2 millimeters, and area 20 square centimeters one-tenth " Banana Type " transparent or semitransparent color is white, is solid finished product.
5, as needed the goods of different size, transformation die size size and shape requires to can be made into that the templates such as polygonal, rib type, round, stripe shape process as stated above is finished product according to breast enlargement goods.
Embodiment four
1, the making of medical augmentation rhinoplasty goods: to extract 100 grams, tail muscle with every 5 be that 1 wisp colligation is put into enzymatic vessel and carried out enzymolysis by extracting tail muscle method in embodiment one, add bio protease S114,450 units/gram, add 0.001 gram/gram, calcium chloride again and adjust that pH value is 6.5 constant temperature, 32 DEG C of enzymolysis, enzymolysis time is 4 hours, enzymolysis terminates.
2, the tail muscle after 4 hours enzymolysis is taken off enzyme cool to half-dried in sterilizing room, receiving moisture content 50%, is semi-finished product.
3, augmentation rhinoplasty modelling rustless steel mold forming, setting upper and lower mould steel plate groove and patrix as convex top with corrosion resistant plate is concavo-convex intersecting parallels in length and breadth.Dark 2 millimeters of groove, wide 1 millimeter.Patrix is convex rise be less than that groove is wide 0.1 millimeter for 1.2 millimeters, wide 0.9, well between word spacing 2 millimeters, long 15 centimetres.
4, semi-finished product are put into template well word in length and breadth in groove, mold 1.2 kilograms of forming under the pressure in contract, airing is finished product, finished product be well word netted, every root muscle diameter stem is 0.8 millimeter, wide 1 millimeter, mesh products area length is 15 centimetres, wide 8 centimetres, area 120 square centimeters, used time amount type is cut.
5, as needed different size size augmentation rhinoplasty goods, carrying out the applicable goods of cutting or measure body doing mould in 120 square centimeters of goods, according to the concrete shape of the bridge of the nose that varies with each individual, from newly establishing mould, by this operation, be finished product.
Claims (7)
1. a human-body-absorbable medical face-lifting product preparation method, utilize nutria tail muscle by under the zymolysis of bio protease, be convenient to molding can manufacture all types of skin-grafting epithelium of needed by human body, repair the face-lifting goods of Tendon Defection, the lamellar of augmentation rhinoplasty or breast enlargement, strip or liquid state, it is characterized in that: nutria tail muscle is sent in enzymatic vessel, add bio protease and carry out enzymolysis, enzyme concentration is 150 units/gram to 1200 Dan Wei ∕ gram, assist enzymolysis must add 0.001 gram/gram, calcium chloride, sodium fluoride 0.002 gram/gram to 0.02 gram/gram must be added and adjust pH value to be 4-7.
2. a kind of human-body-absorbable medical face-lifting product preparation method according to claim 1, is characterized in that: enzymolysis constant temperature is at 25 DEG C-45 DEG C, and enzymolysis time is 3-72 hour.
3. a kind of human-body-absorbable medical face-lifting product preparation method according to claim 2, is characterized in that: add Sal 10-15% in time in the enzymolysis later stage, changes the electrolyte balance of tail muscle, promotes tail muscle inside to unite structural degradation, decomposes tail muscle further.
4. a kind of human-body-absorbable medical face-lifting product preparation method according to claim 3, it is characterized in that: tail muscle is entered emulsifying in mortar machine, through emulsifying tail muscle fibre diameter at 0.0001-0.1 millimeter, tail muscle fiber hydrolization must be thinner, and it is good that its goods are more absorbed by the body.
5. a kind of human-body-absorbable medical face-lifting product preparation method according to claim 4: the nutria oils and fats adding 5% participates in the emulsifying of tail muscle, is the liquid product of less than 0.001 millimeter by the emulsifying of tail muscle to diameter.
6. human-body-absorbable medical face-lifting product preparation method according to claim 1, makes all types of goods, it is characterized in that: use mechanical compaction, pressure is 0.1 kilogram to 1.2 kilograms, is solid-state product.
7. the human-body-absorbable medical face-lifting product preparation method according to any one in claim 1,2,3, it is characterized in that: described bio protease, be selected from the one in aspergillus terricola 3942 protease, bacillus subtilis 1398 protease and bacillus subtilis S114 protease.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110315443.6A CN102350007B (en) | 2011-10-18 | 2011-10-18 | Preparation method of human-body-absorbable medical face-lifting product |
| PCT/CN2012/083059 WO2013056645A1 (en) | 2011-10-18 | 2012-10-17 | Method for preparing absorbable medical aesthetic product |
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| CN201110315443.6A CN102350007B (en) | 2011-10-18 | 2011-10-18 | Preparation method of human-body-absorbable medical face-lifting product |
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| CN102350007B true CN102350007B (en) | 2015-03-04 |
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| CN102350007B (en) * | 2011-10-18 | 2015-03-04 | 崇州市地龙海龙生物制品开发研究所 | Preparation method of human-body-absorbable medical face-lifting product |
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| CN1508154A (en) * | 2002-12-18 | 2004-06-30 | 和康生物科技股份有限公司 | Preparation method of injectable collagen, product and application thereof |
| EP1484070A1 (en) * | 2003-06-05 | 2004-12-08 | Baxter International Inc. | Compositions for repairing and regenerating human dura mater |
| CN101927032A (en) * | 2010-08-04 | 2010-12-29 | 陈启忠 | Dyeing preparation method of surgical sutures produced by tail tendons of nutria |
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| IT1318663B1 (en) * | 2000-08-02 | 2003-08-27 | Mediolanum Farmaceutici Srl | TWO-SIDED COLLAGEN MEMBRANES, ONE OF WHICH ORGANIZED AT THE MACRO-MOLECULAR LEVEL. |
| WO2006029571A1 (en) * | 2004-09-14 | 2006-03-23 | The University Of Hong Kong | Photochemically crosslinked collagen scaffolds and methods for their preparation |
| KR100676285B1 (en) * | 2005-03-11 | 2007-01-30 | 세원셀론텍(주) | Method for isolating collagen from various tissues of animal and preparing collagen solution and matrix produced using the same |
| CN101230088B (en) * | 2008-02-22 | 2011-08-03 | 四川大学 | Method for extracting undenatured natural collagen from animal skin or/and tendon |
| CN102350007B (en) * | 2011-10-18 | 2015-03-04 | 崇州市地龙海龙生物制品开发研究所 | Preparation method of human-body-absorbable medical face-lifting product |
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2011
- 2011-10-18 CN CN201110315443.6A patent/CN102350007B/en not_active Expired - Fee Related
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2012
- 2012-10-17 WO PCT/CN2012/083059 patent/WO2013056645A1/en active Application Filing
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| CN1508154A (en) * | 2002-12-18 | 2004-06-30 | 和康生物科技股份有限公司 | Preparation method of injectable collagen, product and application thereof |
| EP1484070A1 (en) * | 2003-06-05 | 2004-12-08 | Baxter International Inc. | Compositions for repairing and regenerating human dura mater |
| CN101927032A (en) * | 2010-08-04 | 2010-12-29 | 陈启忠 | Dyeing preparation method of surgical sutures produced by tail tendons of nutria |
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| 狸獭油在制剂领域的应用价值;高永春;《佳木斯医学院学报》;19971231;第20卷(第2期);期刊第46页 * |
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| CN102350007A (en) | 2012-02-15 |
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