CN102268084A - Method for preparing soft golded collagen and study on functions thereof - Google Patents
Method for preparing soft golded collagen and study on functions thereof Download PDFInfo
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Abstract
The invention relates to a method for preparing soft golden collagen and study on functions thereof. The method comprises: washing soft tissue skin of animals such as sheep and cattle, which serves as a raw material, subjecting the raw material to acid and alkali treatment, heating and steaming the treated raw material, soaking the steamed material in alcohol, drying, crushing and extracting collagen from soft tissues; and treating the soft tissue collagen to obtain golden soft solid particles. The method has the advantages of simple process flow, low equipment cost, easy configuration and operation, low energy consumption and high yield. According to the result of the study on the functions of the soft golden collagen, the collagen can be used as a health-care product to promote digestion and absorption and improve immunity in human bodies; when used as a component of cosmetics, the collagen can help maintain beauty and keep young and care skin; and the collagen can also be used for providing energy for body functions and can be used as a repair medicine to repair damaged skin (scald, burn, wound and scratch) and the like. Meanwhile, the collagen in the product has high water solubility and therefore can be absorbed quickly by children and middle and old people; and the collagen can be used as a food raw material to develop collagen ice cream, collagen functional beverages and the like.
Description
[technical field]
The present invention relates to a kind of technology of preparing and functional study field of soft gold collagen protein, specifically, is to adopt animal soft tissue epidermises such as ox, sheep to extract the technology of preparing of collagen protein and the research of its function.
[background technology]
Collagen protein is the structural protein of extracellular matrix, and molecular weight is 300ku.The most general constitutional features of collagen protein is a triple-helix structure.It is made up of 3 a chain polypeptide, and each bar collagen chain all is the left hand helix configuration.Article 3, left hand helix chain fork is wound in the right-handed helix structure mutually, and promptly superhelix closes the triple helices structure of collagen protein uniqueness, makes its molecular structure highly stable, and has reduced immunogenicity and excellent biological compatibility.Collagen protein is the rich in protein of people's in-vivo content, accounts for more than 30% of whole body total protein, and it is rich in glycine, proline(Pro) and oxyproline that human body needs.3 kilograms of collagen proteins are arranged in the adult health approximately, mainly be present in human body skin, bone, eyes, tooth, tendon, internal organ positions such as (comprising the heart, stomach, intestines, blood vessel), its function is to keep the form and the structure of skin and histoorgan, also is the important source material material of repairing each damaged tissue.In the human body skin composition, formed by collagen protein by 70%.
At present, manually extract collagen protein mainly from natural biological.The collagen protein toxicological harmless that extraction obtains, no hormone does not have advantages such as corrosion.The present invention attempts extracting collagen protein first from the animal soft tissue epidermis, it is convenient to extract, equipment is simple to operation, the collagen protein purity height that obtains, the soft gold collagen protein that obtains after the extraction: total protein content 92.2%, can be soluble in water rapidly, contain 18 kinds of free amino acid, do not have toxic side effect, help absorption of human body.
The function introduction of collagen protein:
1. the function of collagen protein aspect makeup:
Collagen protein and hydrolyzate thereof are similar to the human skin collagen structure, and consistency is good, exist interactions such as ionic linkage, hydrogen bond, can be spread to the deep layer of skin, and people's skin is had good trophism.Simultaneously owing to contain a large amount of hydroxyls in its molecule, so it has goodish moisture-keeping function, can reach the purpose that keeps skin moist.Amino in the collagen molecules and carboxyl make it have certain surfactivity, can be compatible with other material fine, and skin do not had hormesis, skin aging there is good opsonization.Countries such as the U.S., Japan, Germany, France are added with collagen protein in many cosmetics of super quality, even inject collagen protein to keep skin smooth the human face, and are soft fine and smooth, reduce wrinkle.Collagen protein is major function aspect makeup: wrinkle resistant, preserve moisture, clean skin, whiten, reparation etc.
Collagen protein class cosmetic product performance gentleness, function uniqueness, safe in utilization, the trend that meets contemporary makeup.At present, nearly 50 kinds of world market collagen protein class makeup, China has only a few product such as N-acidylate collagen protolysate condenses, sarkosine, crin propylhomoserin and hyaluronic acid to sell.
2. the function of collagen protein aspect food:
Collagen protein can be used as food or foodstuff additive, after people are edible, can make skin increase elasticity, and strong muscles and bones is built up health.Edible collagen generally derives from skin corium, tendon and the osso-albumin of animal, belongs to the nature renewable resources, and the source is abundant, is a kind of ideal green food.Studies show that collagen protein can reduce blood triglyceride and cholesterol, and can increase the essential trace element of some shortage in the body, thereby it is maintained within the normal relatively scope, is a kind of ideal reducing weight and blood fat food.
The collagen polypeptide of collagen hydrolysate can be added in the bread, and addition is 3%～5%, and the digestion time that can prolong bread improves the volume of bread and the mellowness of bread.
Collagen protein can be used as food raw material, as: collagen protein functional beverage, collagen protein ice cream or the like.
Collagen protein is used as meat products modifying agent as foodstuff additive in meat product, add the tender degree that can improve reticular tissue in the meat product, makes it have good quality, and not only mouthfeel had been well but also nutritious to increase protein content.
At present, more ripe abroad than domestic research, at home, the superiority of collagen food does not also obtain human consumer's abundant approval, but along with the raising of people's living standard and the requirement of green food, low fat high-protein food was constantly carried, collagen food can more and more obtain human consumer's favor.
3. the health-care function of collagen protein aspect the biology guarantor:
(1) improves immunity function
The height of the immunizing power of human body is that to be called the living body molecule of immunoglobulin (Ig) relevant with some, as lymph corpuscle and scavenger cell, immunocyte can be put up a resistance to external foreign matter when external foreign body intrusion, the foreign matter encirclement is engulfed, even self-sacrifice is healthy to keep, and can increase the immunity function that helps improving human body self so the immunocyte number improves.When immune protein combined with collagen protein, the human immune system can increase the immunizing power that adds more than at least one hundred times.When collagen protein was not enough, not only skin and bone can go wrong, and also can have a negative impact to internal organs.That is to say that collagen protein is to keep the indispensable important component of health normal activity.Simultaneously also be to make health keep young, prevent the aged material.
(2) activation muscles and bones, treatment of arthritis and aching
Collagen protein extensively is stored in muscle and muscles and bones, and be the main doctor of giving birth to material such as artificial skin, artificial ligament, joint, so when aching in human body muscles and bones, joint etc., even during inflammation, just can bring into play the effect that it is replenished, changes if use collagen protein, slight illness such as muscles and bones there is the mitigation effect, existing many in this respect clinical medicine reports.
4. the effect of collagen protein aspect medical:
Collagen protein can produce the blood coagulation phenomenon with the thrombocyte effect, and collagen protein quick-acting haemostatic powder bandage can stop artery within 10 to 15 seconds bleeds, and is effective especially when first aid and treatment health wound.Present first aid hemostasis method is except exerting pressure to wound, without any effective means.The protocollagen product can enter huge first-aid supply and military medical market.
Collagen protein is golden yellow flexible glue particle among the present invention, and green non-pollution has no side effect.
[summary of the invention]
The objective of the invention is to from the animal soft tissue epidermis, extract collagen protein first.Collagen protein purity is good, the protein content height.Collagen protein technical process of the present invention is simple.
Of the present invention being contemplated that: for the external at present a lot of countries of the understanding of collagen protein for example: Japan, the U.S., Korea S, France, Germany etc. have very high understanding.But the domestic market has only several prods listing seldom, and the collagen protein protein content of generally producing is not high.The domestic common people lag far behind external to collagen protein understanding, understand very few to the function of collagen protein.Consider that at present this material has very big development prospect and the big market outlook of diving.The present invention attempts first adopting from the animal soft tissue epidermis and extracts collagen protein, obtaining is the protein content height, has no side effect, and meets the various functional requirements of human body fully through functional study, promptly can also can be used as the exploitation of healthcare products and skin care product as drug development.The inventive method is easy to simply realize that processing unit is simple to operation that whole technological process is pollution-free, is green biological extraction and preparation process fully.
The objective of the invention is to be achieved through the following technical solutions:
One. sheep or ox soft tissue epidermis stripping process: selected healthy blue or green strong sheep or Niu Zuowei alternative, in sheep or ox body, peel off grain through slaughtering the back, muscle and enteron aisle, wash cycles, 1～2 day drying is placed in the alcohol sterilization, and clear water soaks and softened in 1 day, use scraper shaving soft tissue, obtain transparent gel-like film cortex and be soft gold collagen protein starting material.
Two. soft gold collagen protein preparation process comprises: the soft tissue epidermis of sheep or ox cleans up, according to 1: 3 ratio of water, add alkali, add salts contg 2～7%, it is (7.0～7.6) that pH value is regulated in acid, heated 4 hours at 80～87 ℃, put into filter pocket and filter, alcohol-pickled, pulverize behind the oven drying at low temperature.
Compared with prior art, positively effect of the present invention is: collagen protein total protein content very high detection is reported as 92.2%, contains 18 seed amino acids of needed by human body, and is water-soluble easily, is easy to absorption of human body.
Soft gold collagen protein of the present invention, the purity of protein height does not contain hormone, the heavy metal free ionic soil, green pure natural extracts, and helps absorption by human body.Soft tissue is peeled off simple, and collagen protein preparation technology equipment is simple, is easy to realize and operation.
[description of drawings]
Figure one soft tissue epidermis is peeled off schema
Figure two soft gold collagen protein preparation technology schemas
[embodiment]
The embodiment of a kind of soft gold collagen protein preparation method of the present invention and functional study below is provided.
Embodiment 1.
Soft tissue epidermis stripping process: selected healthy ox and sheep are as extracting object, in ox and sheep body, peel off grain, muscle and enteron aisle through slaughtering the back, clean, the alcohol sterilization, place 2 days dryings, clear water soaks and softened in 1 day, and use scraper shaving soft tissue obtains transparent wire and netted gel-like film cortex is the soft tissue epidermis.
Soft gold collagen protein preparation process comprises: selected animal soft tissue epidermis (grain, muscle, enteron aisle etc.) is cleaned up, according to soft tissue and 1: 3 ratio of water, add alkali, add salts contg 5%, regulate with acid, measuring pH value is neutral (7.2), and thermometer is measured 84 ℃ of constant temperature 4 hours, puts into filter pocket and filters, alcohol 55 degree soak, and oven drying at low temperature machine crushed after being dried obtains soft golden yellow solid particulate and is soft gold collagen protein.
The functional study of soft gold collagen protein:
(1) principal component analysis: total protein content 92.2%, soluble in water, be rich in 18 kinds of free amino acid; Suitable human consumption absorbs.
(2) toxicity test: mouse experiment LD50＞20000mg/kg; Test-results: nontoxic.
(3) biological small white mouse experiment: under the same living condition, select with the small white mouse of product series a pair of; One is used normal diet to feed, and other one is added soft gold collagen protein in normal diet; Pass through environmental experiment (UV-irradiation) respectively, fatigue experiment (motion ceaselessly in a large number), injection experiment (alcohol injection) after collagen protein has been taken in checking, can improve the anti-fatigue performance of mouse human body, strengthens body immunity.
Soft gold collagen protein functional development direction:
(1) as building up health and improving the healthcare products of immunizing power, for example: collagen protein powder, capsule and oral liquid or the like.
(2) as wound healing and wound repair drug development, for example: the collagen protein styptic powder, collagen protein is repaired ointment or the like.
(3) develop as the additive component of makeup.
(4) for example: as the development and use such as raw material of novel ice cream and functional beverage as the starting material of food and addition material; Can share with bifidus bacillus etc., be added in the sour milk;
Claims (7)
1. the preparation of a soft gold collagen protein and functional study is characterized in that mainly selecting for use strong, young, healthy animal soft tissue as the raw material that extracts soft gold collagen protein.For example: muscle, ligament, tendon, grain etc.Animal soft tissue leaching process: selected strong, young, healthy animal → separate soft tissue → immersion → cleaning → shaving soft tissue → extraction soft tissues such as fresh muscle, ligament.Soft gold collagen protein preparation process comprises: animal soft tissue → cleaning animal soft tissue → animal soft tissue and water are by adding alkali at 1: 3, being bordering on neutrality → 80～87 ℃ → filter vat of heating filtration → alcohol-pickled → oven drying at low temperature → freezing preservation with salt 2～7%, pH value.Functional study comprises: strengthening immunity, for physical function energy, beautifying face and moistering lotion skin care be provided, repair injured skin (scald, burn, incised wound, scratch), as food raw material (beverage, ice cream) etc.
2. the preparation of a kind of soft gold collagen protein as claimed in claim 1 and functional study, processing unit and technical parameter that it is described are as follows:
Animal is chosen: selected health, blue or green strong ox, sheep are extracted object as main soft tissue.
Animal soft tissue is soaked: soft tissue: water (1: 3)
Salt concn: 2～7%
Acid-alkali treatment: PH=7.0～7.6
Temperature control: 80～87 ℃
Processing unit: reaction boiler, fermentation vat, scraper, dryer, refrigerator, or the like.
3. according to claim 1, a kind of preparation method of soft gold collagen protein, described preparation process is:
(1) animal is selected: select for use healthy blue or green strong ox and sheep to extract object (mainly being: ox and sheep) as soft tissue.
(2) separation of soft tissue epidermis: extract animal soft tissue, use the high-temperature sterilization water coolant to clean soft tissue, distilled water immersion is softening, what the use scraper was careful peels off out with muscle, grain and enteron aisle epidermis from the animal health, after the epidermis peeled off is clear once more, the sterilization, put into the dryer oven drying at low temperature.
(3) extraction of soft gold collagen protein: 1: 3 ratio of animal soft tissue epidermis and water, add alkali, salts contg 2～7%, it is (7.0～7.6) that pH value is regulated in acid, 80～87 ℃ of constant temperature 4 hours, putting into filter pocket filters, alcohol-pickled sterilization, pulverize the oven dry back, obtains soft faint yellow collagen protein grain.
4. as described in the claim 2: selected ox of a kind of preparation of soft gold collagen protein and sheep.
5. as described in the claim 3: in a kind of soft gold collagen protein leaching process, need be with animal soft tissue: epidermises such as muscle, enteron aisle be peeled off out, soak sterilization, clean oven dry and softening.
6. as described in the claim 3: among a kind of soft gold collagen protein preparation technology, make respectively to wash with water, the acid-alkali treatment pH value is (7.0～7.6), and temperature is provided with 80～87 ℃, and 4 hours, alcohol-pickled and oven dry was pulverized.
7. according to claim 1: a kind of functional study of soft gold collagen protein proves that soft gold collagen protein of the present invention is at strengthening immunity, for physical function provides energy, beautifying face and moistering lotion skin care, aspects such as (scald, burn, hurt, scratch) has very high researching value and potentiality to be exploited to repair injured skin.The collagen protein of this product has the well water-soluble children of being adapted to and middle-aged and old rapid absorption simultaneously, can be used as food raw material, develops collagen protein ice cream and collagen protein functional beverage.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016113613A1 (en) * | 2015-01-16 | 2016-07-21 | Welldrinks Ltd | Beverage with collagen and additional additives |
| CN111345390A (en) * | 2020-03-20 | 2020-06-30 | 江西师范大学 | Ice cream premixed flour rich in collagen and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101628938A (en) * | 2009-08-12 | 2010-01-20 | 江苏省海洋资源开发研究院 | Extraction technology of macromolecular collagen protein |
| CN101805775A (en) * | 2010-04-12 | 2010-08-18 | 赵雨 | Method for preparing collagen from deer sinew |
| CN102020711A (en) * | 2010-11-05 | 2011-04-20 | 马兴付 | Casing smooth muscle collagen preparation method and functional study |
-
2011
- 2011-06-07 CN CN2011101500364A patent/CN102268084A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101628938A (en) * | 2009-08-12 | 2010-01-20 | 江苏省海洋资源开发研究院 | Extraction technology of macromolecular collagen protein |
| CN101805775A (en) * | 2010-04-12 | 2010-08-18 | 赵雨 | Method for preparing collagen from deer sinew |
| CN102020711A (en) * | 2010-11-05 | 2011-04-20 | 马兴付 | Casing smooth muscle collagen preparation method and functional study |
Non-Patent Citations (1)
| Title |
|---|
| JEROME GROSS等: "Extraction of collagen from connective tissue by neutral salt solutions", 《PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES》, vol. 41, no. 1, 15 January 1955 (1955-01-15), pages 1 - 7 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016113613A1 (en) * | 2015-01-16 | 2016-07-21 | Welldrinks Ltd | Beverage with collagen and additional additives |
| CN107205448A (en) * | 2015-01-16 | 2017-09-26 | 威尔君克斯有限公司 | Beverage containing collagen and additional additives |
| GB2550510A (en) * | 2015-01-16 | 2017-11-22 | Welldrinks Ltd | Beverage with collagen and additional additives |
| RU2719651C2 (en) * | 2015-01-16 | 2020-04-21 | Веллдринкс Лтд | Beverage with collagen and additional additives |
| CN111345390A (en) * | 2020-03-20 | 2020-06-30 | 江西师范大学 | Ice cream premixed flour rich in collagen and preparation method thereof |
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Application publication date: 20111207 |