CN102007917B - Composite bactericide and application thereof - Google Patents

Abstract

The invention discloses a compound fungicide, whose components and weight content are: 5% to 90% of procymidone and 10% to 95% of component B, where component B is difenoconazole and tebuconazole alcohol, triadimefon, propiconazole or myclobutanil. The invention also simultaneously discloses an emulsion-type bactericide made by using the compound bactericide, and each L of the emulsion-type bactericide contains 50-900 g of the compound bactericide. The invention also simultaneously discloses a powder/granule type fungicide made by using the above-mentioned compound fungicide, and the weight content of the compound fungicide in the powder/granule type fungicide is 10%-90%. The compound fungicide can be used to prevent and control fungal diseases of vegetables, fruit trees or rapeseed.

Description

Compound fungicides and their uses

technical field

The invention relates to a fungicide, in particular to a compound fungicide for preventing and treating diseases of vegetables, fruit trees and rapeseed.

Background technique

Procymidone [procymidone, N-(3,5-dichlorophenyl)-1,2-dimethylcyclopropane-1,2-dicarbonylimide] is effective against Botrytis sp. It has a good control effect on plant diseases caused by various pathogenic fungi such as Alternaria and Alternaria. Studies have shown that the agent inhibits fungal growth by activating the fungal two-component histidine kinase signaling pathway. Phosphorylation levels of OS-2 in fungi such as Botrytis cinerea and Colletotrichumlagenarium increased rapidly after chemical treatment, and the TCHK signaling pathway of fungi was continuously activated, resulting in a large amount of glycerol accumulation in fungi. eventually lead to cell death.

Procymidone is a broad-spectrum contact-killing modern protective fungicide launched in the early 1970s. It is another type of special-effect fungicide after benzimidazole fungicides for the control of botrytis and other diseases. The main problem in the current production of dicarboximide fungicides such as procymidone is the occurrence of drug resistance. In 1983, Beever et al detected the existence of resistant strains of Botrytis cinerea in the field. Alternaria alternate developed drug resistance in less than 5 years after drug control. Important plant pathogenic fungi such as domestic vegetables, strawberry cinerea, Moniliniaf ructicola, Sclerotinia homoeocarpa and Sclerotium cepivorum have all been effective against diformyls such as procymidone Imine fungicides produce field resistance. In addition, double-resistant strains that are resistant to both benzimidazole fungicides and procymidone have appeared in the field, which seriously affects the effective use of procymidone. In addition, the lack of sufficient therapeutic effect also limits the application of procymidone. At present, the main strategies to delay and control the development of fungicide resistance include reducing the use of fungicides, compounding or rotating use of fungicides with different mechanisms of action.

Contents of the invention

The technical problem to be solved by the present invention is to provide a compound fungicide that can be used to prevent and control vegetable, rape and fruit tree diseases, and the compound fungicide can especially be used to prevent and control drug-resistant gray mold.

In order to solve the above-mentioned technical problems, the present invention provides a compound bactericide whose components and weight content are: 5% to 90% of procymidone (component A) and 10% to 95% of (component B) , Component B is difenoconazole or tebuconazole, triadimefon, propiconazole or Myclobutanil.

The present invention also simultaneously provides an emulsion-type bactericide made by using the compound bactericide, and each L of the emulsion-type bactericide contains 50-900 g of the compound bactericide.

The present invention also provides a powder/granule type fungicide made by using the above-mentioned compound fungicide, and the weight content of the compound fungicide in the powder/granule type fungicide is 10%-90%.

The present invention also provides the use of the above-mentioned compound fungicide at the same time: it is used for preventing and treating fungal diseases of vegetables, fruit trees and rapeseed.

As the improvement of the purposes of the compound fungicide of the present invention: the fungal disease is vegetable gray mold, vegetable leaf mold, vegetable sclerotinia, vegetable early blight, strawberry and grape gray mold, fruit tree dry rot or rape sclerotia sick.

In actual use, the compound fungicide of the present invention can be processed into various pesticide preparations with different contents and formulations, such as emulsion-type fungicides and powder/granule-type fungicides, according to different control objects. Each L of emulsion-type fungicide contains 50-900g of compound fungicide, 1%-45% of solvent (including emulsifier), 1%-20% of auxiliary agent, and the rest is water; the above percentages are the ingredients in the emulsion Volume content in type fungicides. The components and weight content of the powder/granule fungicide are: 10%-90% compound fungicide, 9%-75% adsorbent or filler and 1%-20% auxiliary agent.

Due to the different content of components, emulsion fungicides can be divided into suspension concentrate (SC), emulsion in water (EW) and microemulsion (ME), and powder/granular fungicides can be divided into wettable powder (WP) and water Dispersible Granules (WG). The various formulations of the above-mentioned compound fungicides are all prepared from the compound fungicides using conventional production processes, and the emulsifiers, solvents, auxiliaries, and adsorbents/fillers involved in the components are all produced during the preparation of pesticides. conventional varieties. For example: Nongru 33#, Nongru 700# or Nongru 602# can be used as emulsifier, xylene, DMF or ethanol can be used as solvent, diatomite, white carbon black or perlite can be used as adsorbent/filler , Auxiliaries can choose silicone, polyethylene glycol, etc.

The present invention aims at the situation that procymidone, the backbone agent for preventing gray mold and sclerotinia used in the existing production, has high efficiency and low price but poor therapeutic effect and drug resistance has appeared; Azole, etc. have the characteristics of no cross-resistance with procymidone, low toxicity, safety, good environmental compatibility, and good therapeutic effect, providing a set of control methods for vegetables, fruit trees, and rapeseed diseases (especially drug-resistant botrytis cinerea) Compound fungicide. By combining the above-mentioned fungicides with different mechanisms of action—procymidone (component A) and component B (component B is difenoconazole, tebuconazole, triadimefon, propiconazole or myclobutanil) A kind of) carry out compounding, foster strengths and offset weaknesses, reach the purpose of strengthening therapeutic effect, improving effect, delaying and controlling drug resistance. The compound fungicide of the present invention can be used to prevent and control vegetable gray mold, vegetable leaf mold, vegetable sclerotinia, vegetable early blight, strawberry and grape gray mold or fruit tree fungal canker, fruit tree dry rot and rape Sclerotinia and other fungal diseases are new, high-efficiency, low-toxic fungicides.

The inventor provides the technical and theoretical basis for the present invention through the research on the occurrence, evolution and control technology of resistance to Botrytis cinerea and the antibacterial mechanism and resistance of procymidone. The present invention is compounded by using one of procymidone (component A) and component B (component B is difenoconazole or tebuconazole or triadimefon or propiconazole or myclobutanil) Screening, whose goal is to control drug resistance, reduce production costs, and improve therapeutic effects. The invention mainly prevents and treats botrytis cinerea, early blight and sclerotinia against procymidone. The invention meets the needs of the current production of green and pollution-free agricultural products.

Procymidone in the compound preparation has high activity against Botrytis cinerea, etc., and the cost is very low but lacks therapeutic effect and has produced drug resistance; difenoconazole, tebuconazole, triadimefon, propiconazole and Cinconazole is a high-efficiency agent (for botrytis cinerea, early blight and sclerotinia, etc.), especially it has no cross-resistance with procymidone and has a high therapeutic effect. After procymidone and difenoconazole or tebuconazole or triadimefon or propiconazole or myclobutanazole are compounded, the drug effect is significantly increased, and the production cost of the present invention is higher than that of using alone Conazole, triadimefon, propiconazole, cloconazole or procymidone should be reduced by 15% to 30%, and the control effect on gray mold can be stabilized at more than 70%.

The results of indoor bioassay and field efficacy test of the present invention show that the two components are compatible and compatible, safe to use, remarkable in effect, and can effectively prevent botrytis and other diseases. Compared with other pesticides, the present invention has the following advantages:

1. The formula product of the present invention is a high-efficiency, low-toxicity, low-residue, and environmentally compatible pesticide, which has both protective and therapeutic effects, and meets the requirements of green agricultural product production and environmental protection.

2. In addition to solving the problem of resistance to procymidone such as Botrytis cinerea, the present invention can also effectively solve the problem of unsatisfactory treatment effect of procymidone.

3. The method of using the product of the present invention is simple, the cost is low, and all farmers can accept it.

4. The present invention is safe for crops, and has good effect of preventing and treating Botrytis cinerea, etc., and the effect can be stabilized at more than 70%, and it is not easy to produce drug resistance.

When the compound fungicide of the present invention is actually used in the field, use 5 to 50 grams of active ingredients per mu (this data is the amount of the compound fungicide, i.e. the effective amount of the emulsion type fungicide or powder/granule type fungicide). When using, the preparation of this compound fungicide is diluted with water to an effective concentration of 0.01-5 g/L, and the method of use is foliar spraying.

In order to obtain the compound bactericide of the present invention, the inventor once carried out following experiment:

Experiment 1. Indoor formula screening:

The determination of toxicity and synergistic coefficient is to dissolve the former medicine of procymidone and difenoconazole in acetone respectively according to different weight ratios, adopt the mycelia growth method to measure the inhibitory effect on Botrytis cinerea (Table 1), and then calculate EC ₅₀ values and synergistic coefficients. According to the main control objects, the ratio of synergistic coefficient (SR) ≥ 1.5 can be selected, and appropriate dosage forms can be processed.

Table 1. Screening of formulas for preventing and treating Botrytis cinerea

Note: The above brackets refer to the weight ratio of the two components.

Experiment 2. Indoor formula screening:

Toxicity and synergistic coefficients are determined by dissolving the procymidone and tebuconazole in different weight ratios in acetone respectively, using the mycelium growth method to measure the inhibitory effect on tomato leaf mold (Table 2), and then calculating the EC ₅₀ value and synergy factor.

Table 2. Formula screening for preventing and controlling tomato leaf mold

Note: The above brackets refer to the weight ratio of the two components.

Experiment 3. Indoor formula screening:

Toxicity and synergistic coefficients are determined by dissolving procymidone and triadimefon in acetone at different weight ratios, using the mycelium growth method to measure the inhibitory effect on Sclerotinia sclerotiorum (Table 3), and then calculating the EC ₅₀ value and synergy factor.

Table 3. Formula screening for preventing and controlling eggplant sclerotinia

Note: The above brackets refer to the weight ratio of the two components.

Experiment 4. Indoor formula screening:

Toxicity and synergistic coefficients are determined by dissolving the procymidone and propiconazole in acetone respectively in different weight ratios, using the mycelium growth method to measure the inhibitory effect on Sclerotinia sclerotiorum (Table 4), and then calculating the EC ₅₀ value and synergy factor.

Table 4. Formula screening for preventing and controlling Sclerotinia sclerotiorum

Note: The above brackets refer to the weight ratio of the two components.

Experiment 5. Indoor formula screening:

Toxicity and synergistic coefficients are determined by dissolving procymidone and cycloconazole in acetone respectively in different weight ratios, using the mycelium growth method to measure the inhibitory effect on cucumber anthracnose bacteria (Table 5), and then calculating the EC ₅₀ value and synergy factor.

Table 5. Screening of formulas for controlling cucumber anthracnose

Note: The above brackets refer to the weight ratio of the two components.

Detailed ways

Embodiment 1, a compound fungicide, is made up of 75% procymidone and 25% difenoconazole; above-mentioned % is weight %.

Above-mentioned compound fungicide is made effective weight content and is the suspending agent (SC) of 80%, promptly contains the compound fungicide of 800g in every L suspending agent, the solvent (glycerol) of 45mL, the auxiliary agent (alkane) of 70mL base naphthalene sulfonate, methyl naphthalene sulfonate sodium formaldehyde condensate and silicone defoamer), and the rest is deionized water. According to the formula requirements, fully mix the above-mentioned original medicine, additives, solvents, deionized water, etc., put it into a high-shear homogeneous emulsifier for high-speed shearing, and then pump it to a sand mill for sanding, and then get a suspension after filtering agent. This suspending agent is hereinafter referred to as 80% rot·benzene SC.

Field efficacy test:

The above-mentioned suspending agent (80% rot·benzene SC) was used to control strawberry botrytis. The experiment was set in the suburbs of Hangzhou City, each test area was 0.50hm ² , and the test objects were strawberries with the same growth conditions. Spray the medicine for the first time on March 10, 2008, spray the medicine for the second time on March 20, spray 2 times altogether. The application rates were 170 and 350mL per hectare of 80% Hum·Benzene SC, respectively. The control agent I was 50% procymidone WP 600g per hectare; the control agent II was 25% difenoconazole EC 500mL per hectare.

The control effects are shown in Table 6. The control effects of 170 and 350 mL of 80% rot·benzene SC per hectare on Botrytis cinerea were 72.95% and 82.37%, respectively. Therefore, under the premise of the same effective dosage, the present invention is superior to the contrast agent, and the difference is significant.

Table 6. Effect of 80% rot·benzene SC on controlling gray mold of strawberry

*: Different lowercase letters behind the value in the same column indicate significant difference at P=0.05 level.

Embodiment 2, a compound bactericide, is made up of 10% procymidone and 90% tebuconazole; above-mentioned % is weight %. Above-mentioned compound bactericide is made effective weight content and is the wettable powder (WP) of 50%, promptly contains the compound bactericide of 500g in every kg powder, the auxiliary agent (sodium lauryl sulfate and lignin sulfonate) of 50g sodium carbonate), light calcium carbonate of 35g, and all the other being kaolin. The original drug, various additives and fillers are fully mixed according to the ratio of the formula, and can be obtained after being pulverized by a superfine pulverizer. This wettable powder (WP) is hereinafter referred to as 50% rottenone WP.

Field efficacy test:

The above-mentioned wettable powder (50% rot·penta WP) was used to control eggplant leaf mold. The experiment was set in the suburb of Zhuji City, each test area was 0.40hm ² , and the test objects were eggplants with the same growth conditions. Spray the medicine for the first time on May 15, 2008, spray the medicine for the second time on May 22, spray 2 times altogether. The application rate is 350g and 500g per hectare of 50% rot.penta WP. The control agent I was 500 g per hectare of 50% procymidone WP. The control agent II was 315g per hectare of 80% tebuconazole WP.

The control effects are shown in Table 7. The control effects of 350g and 500g per hectare of 50% rot·penta WP on leaf mold were 68.14% and 83.09%, respectively. Under the premise of the same effective dosage, the present invention is superior to the contrast agent, and the difference is significant.

Table 750% rot·penta WP control effect of eggplant leaf mold

Pharmacy Repeat 1 Repeat 2 Repeat 3 Repeat 4 average Average control effect (%) 50% rot·pentyl WP 350g/hm ² 0.24 0.38 0.32 0.36 0.325 68.14b ^* 50% rot·pentyl WP 500g/hm ² 0.21 0.15 0.19 0.14 0.1725 83.09a

50% Procymidone WP 500g/hm ² 0.48 0.42 0.46 0.47 0.4575 55.15c 80% Tebuconazole WP 315g/hm ² 0.51 0.47 0.49 0.50 0.4925 51.7c CK (clean water control) 0.95 0.85 1.04 1.24 1.02 /

*: Different lowercase letters behind the value in the same column indicate significant difference at P=0.05 level.

Embodiment 3, a compound bactericide, is made up of 75% procymidone and 25% propiconazole; above-mentioned % is weight %.

Above-mentioned compound bactericide is made effective weight content and be the suspension concentrate (SC) of 40%, promptly contain the compound bactericide of 400g in every L suspension concentrate, the solvent (ethylene glycol) of 50mL, the auxiliary agent (fat) of 65mL alcohol polyoxyethylene ether, polyether defoamer and sodium starch phosphate), and the rest is deionized water. According to the formula requirements, fully mix the original drug, additives, solvents, deionized water, etc., put it into a high-shear homogeneous emulsifier for high-speed shearing, and then pump it to a sand mill for sanding, and filter to obtain a suspending agent . This suspending agent is hereinafter referred to as 40% rot-acid SC.

field efficacy test

The experiment of tomato early blight was carried out with the above-mentioned suspension (40% rot·acid SC). The experiment was set up in the suburbs of Hangzhou City, each test area was 0.40hm ² , and the test objects were tomatoes with the same growth conditions. Spray the medicine for the first time on April 25, 2009, spray the medicine for the second time on May 2, spray 2 times altogether. The application rate is 400 and 650 mL per hectare of 40% rotacin SC. The control agent I was 650 g per hectare of 50% procymidone WP. The control agent II is 62% propiconazole EC 250mL per hectare.

The control effects are shown in Table 8. The control effects of 40% rot-acid SC 400 and 650mL per hectare on early blight were 62.43b% and 74.60%, respectively. Under the premise of the same effective dosage, the present invention is superior to the contrast agent, and the difference is significant.

Table 8. Effect of 40% rot·bing ME on controlling early blight of tomato

Pharmacy Repeat 1 Repeat 2 Repeat 3 Repeat 4 average Average control effect (%) 40% rot propane SC 400mL/hm ² 0.35 0.38 0.42 0.47 0.355 62.43b ^* 40% rot propane SC 650mL/hm ² 0.32 0.25 0.27 0.34 0.24 74.60a 50% Procymidone WP 650g/hm ² 0.54 0.41 0.49 0.62 0.4525 52.12c 62% propiconazole EC 250mL/hm ² 0.38 0.49 0.44 0.37 0.42 55.97c CK (clean water control) 0.86 0.95 0.81 0.94 0.954 /

*: Different lowercase letters behind the value in the same column indicate significant difference at P=0.05 level.

Embodiment 4, a compound bactericide, is made up of 75% procymidone and 25% triadimefon; above-mentioned % is weight %.

Above-mentioned composite bactericide is made effective weight content and is the wettable powder (WP) of 50%, promptly contains the composite bactericide of 500g in every kg powder, the auxiliary agent (polyacrylamide and light calcium carbonate) of 50g, 12g of talc, the rest is kaolin. The original drug, various additives and fillers are fully mixed according to the ratio of the formula, and can be obtained after being pulverized by a superfine pulverizer. This wettable powder (WP) is hereinafter referred to as 50% rot three WP.

Determination of protective and therapeutic effects:

Cucumbers were cultivated in a greenhouse and artificially inoculated with anthracnose bacteria. The foliar spray treatment was carried out 24 hours before inoculation of anthracnose bacteria (protective effect) and 24 hours after inoculation of anthracnose bacteria (curative effect). The medicament treatment is 50% procymidone WP diluted with water to 50mg/L (that is, the compound fungicide containing 50mg in each L), treated with clear water as CK (contrast), and the contrast agent I is 50% procymidone WP 50mg/L L (that is, containing 50 mg of procymidone in every L), and contrast agent II is 25% triadimefon WP 50 mg/L (that is, containing 50 mg of triadimefon in every L). The effective dosage was the same, and the incidence was investigated 7 days after inoculation with anthrax bacteria. The effects are shown in Table 9. The protective and therapeutic effects of 50% rot three WP 50mg/L on cucumber anthracnose were 73.1% and 71.2%, which were better than the two control agents, and the difference was significant.

Table 9. Protective and therapeutic effects of 50% rot three WP on cucumber anthracnose

Pharmacy Protection effect (%) treatment effect(%) 50% rot three WP 50mg/L 73.1 71.2 50% Procymidone WP 50mg/L 54.5 30.3 25% Triadimefon WP 50mg/L 60.1 62.3

Embodiment 5, a compound fungicide, is made up of 50% procymidone and 50% myclobutanil; above-mentioned % is weight %.

It is 50% wettable powder (WP) that above-mentioned compound fungicide is made into effective weight content, promptly contain the compound fungicide of 500g in every kg powder, the auxiliary agent (polyacrylamide and light calcium carbonate) of 75g, 50g of talcum powder, the rest is kaolin. The original drug, various additives and fillers are fully mixed according to the ratio of the formula, and can be obtained after being pulverized by an ultrafine pulverizer. This wettable powder (WP) is hereinafter referred to as 50% rot-nitrile WP.

Determination of drug resistance control effect:

The above-mentioned wettable powder (50% procymidone WP) was used to control the resistance of strawberry gray mold to procymidone. The experiment was carried out simultaneously in the suburbs of Zhuji City and Hangzhou City, each test area was 0.60hm ² , and the test objects were strawberries with the same growth conditions. The first test was sprayed on March 10, 2008, and it was sprayed every 10 days during the epidemic period of strawberry gray mold until May 1, 2010. The application rate is 500g per hectare of 50% rot-nitrile WP. The control agent I was 500 g of 50% procymidone WP per hectare, and the control agent II was 625 g of 40% myclobutanil WP per hectare.

Sampling was taken on March 10, 2008 and May 8, 2010, respectively, and the development of resistance to procymidone of botrytis botrytis before and after treatment was analyzed. The control effect is shown in Table 10. 500g/ha of 50% rot·nitrile WP has a control effect of 48% on the resistance of strawberry gray mold. However, with only 50% procymidone WP 500g per hectare, the control effect on the resistance of strawberry botrytis is -53.25%. This proves that the present invention preferably solves the problem of the resistance of diseases, especially botrytis cinerea, to procymidone.

Table 10. Control effect of 50% rot-nitrile WP on Botrytis cinerea resistance

Finally, it should be noted that the above examples are only some specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and many variations are possible. All deformations that can be directly derived or associated by those skilled in the art from the content disclosed in the present invention should be considered as the protection scope of the present invention.

Claims (1)

1. The compound fungicide is characterized in that the compound fungicide is any one of the following: Composed of procymidone and difenoconazole, the weight ratio of procymidone: difenoconazole=3:1, the compound fungicide is used for preventing and treating Botrytis cinerea; Composed of procymidone and tebuconazole, the weight ratio of procymidone: tebuconazole=1:9, the compound fungicide is used to prevent and treat tomato leaf mold; Composed of procymidone and cycloconazole, the weight ratio of procymidone:myclobutanazole=1:9, the compound fungicide is used for preventing and treating cucumber anthracnose. the