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CN101971921A - Zinc-enriched inactivated lactobacillus microecological agent and preparation method thereof - Google Patents

Zinc-enriched inactivated lactobacillus microecological agent and preparation method thereof Download PDF

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CN101971921A
CN101971921A CN2010105410358A CN201010541035A CN101971921A CN 101971921 A CN101971921 A CN 101971921A CN 2010105410358 A CN2010105410358 A CN 2010105410358A CN 201010541035 A CN201010541035 A CN 201010541035A CN 101971921 A CN101971921 A CN 101971921A
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zinc
lactobacillus
deactivation
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preparation
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CN101971921B (en
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胡文锋
黄艾武
陈斌强
于凌禹
张明
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Biological Source Biotechnology (shenzhen) Ltd By Share Ltd
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Abstract

The invention relates to the field of feed additives and discloses a zinc-enriched inactivated lactobacillus microecological agent and a preparation method thereof. The Zinc-enriched inactivated lactobacillus microecological agent comprises the following components in parts by mass: 30-60 parts of zinc-enriched lactobacillus fermentation product and 40-70 parts of excipient, wherein the zinc enriched lactobacillus fermentation product comprises the components of inactivated lactobacillus cells (108-1010/g), zinc element (0.1-1mg/g), culture medium and water. The zinc-enriched inactivated lactobacillus microecological agent can effectively adjust enteric microecological balance of animals and effectively prevent diarrhea and other enteric diseases of livestock and poultry, wherein the zinc element contained in the zinc-enriched inactivated lactobacillus microecological agent not only can complement the requirement on the growth of animals, but also can reduce the addition level of zinc oxide in feeds for weaned pigs. The zinc-enriched inactivated lactobacillus microecological agent can be used as a livestock and poultry feeding microecological agent for reducing the addition of zinc oxide in feeds for weaned pigs, avoiding zinc poisoning caused by a high-zinc formula, and greatly reducing the risk of environmental pollution caused by zinc residual in pig manure.

Description

Rich zinc deactivation lactobacillus micro-ecological preparation and preparation method thereof
Technical field
The present invention relates to feed additive field, relate in particular to a kind of rich zinc deactivation lactobacillus micro-ecological preparation and preparation method thereof.
Background information
Zinc is the indispensable element of animal, owing to its widely biochemical functions be called as " biological element ".Zinc is the composition of plurality of enzymes, and its participates in the metabolism of the nutriments such as synthetic, fat, carbohydrate and vitamin of body protein, and the generation of skeleton development and cutin also needs the participation of zinc; Zinc can also strengthen immunity of organisms, improves anti-infection ability and promotes wound healing.When lacking zinc, the disorder of body metabolism be can cause, the growth, immunity of animal etc. influenced.
At present, the kind of the zinc of extensive use has two kinds of inorganic zinc and organic zincs in the animal-breeding process.Inorganic zinc mainly comprises: zinc oxide, zinc sulfate and basic zinc chloride etc.Adding inorganic zinc in feed is a comparatively cheap additional zinc element approach, but its absorptivity and bioavailability in animal body is lower, and easy and other mineral matter elements generation antagonistic actions.Organic zinc mainly comprises: complexing zinc, organic chelated zinc, zinc albuminate and polysaccharide zinc complex etc. are several.With respect to inorganic zinc, organic zinc is as the zinc source, and low dosage uses the additional zinc effect that just can obtain the high dose inorganic zinc.
In various zinc source, have only zinc oxide that young animal (as piglet) is not only had the effectiveness of additional zinc element, but also have pharmacological action concurrently, inhibited to pathogenic entero becteria, reach the purpose that prevention and treatment are suffered from diarrhoea.But zinc oxide is absorptivity and bioavailability very low (about below 10%) in animal body, and is subjected to the influence of hydrochloric acid in gastric juice neutralization, therefore must the high level interpolation just can reach obvious effects.Studies have shown that, in feed, add 2500-3500mg/kg zinc oxide, reduce " weaning syndrome " influence weanling pig for promoting weanling pig growth and prevention diarrhoea to have quite significantly effect.At present, in pig industry, use high level of oxidation zinc to be mainly used in the baby pig feedstuff at wean initial stage, play anti-diarrhoea and growth promoting function; When using low dosage zinc, normally with zinc sulfate or zinc-amino acid chelate, main purpose provides the required zinc element of growth.
Use the harm of high level of oxidation zinc to be fully realized by people at present.High level of oxidation zinc can cause piglet " zinc poisoning ", and growth causes negative effect to the piglet late growing stage.Secondly, add the high-level zinc that surpasses 20~30 times of normal needs in the daily ration, must break the balance of original various elements, unfavorable to the absorption of copper, iron, cause animal iron, copper secondary deficiency to occur, anaemia appears.In a single day moreover, if stop to add high-level zinc, change daily ration, piglet can be suffered from diarrhoea again, reason may be that zinc oxide is to the harmful bacterium tool inhibitory action in the enteron aisle.
Animal is low to the bioavailability of common zinc oxide, only with utilize wherein about 10%, most of with defecate in environment.(2004) such as guilt rubine studies show that, after the high dose zinc oxide (3000mg/kg) of feeding was digested and assimilated by the pig body, its, zinc discharge rate was 33 times of basal diet group of feeding every day.Therefore, add high level of oxidation zinc and can cause the wasting of resources and metal residual, and cause the irreversible pollution of environment.
No. 1224 bulletin " the feed addictive safe handling standard " regulation of The Ministry of Agriculture of the People's Republic of China, MOA's issue, behind the weaned piglet in preceding 2 all mixed feeds the addition of the zinc of zinc oxide form be no more than 2250mg/kg, this high zinc daily ration can only be used to the to wean piglet in preceding 2 weeks of back.As seen, country's further restriction and standard zinc oxide use (annotate: having abrogated the middle weanling pig preceding 2 all daily ration zinc oxide additions of agricultural industry criteria " NY 929-2005 " is to be no more than 3000mg/kg).People have recognized the side effect of using high level of oxidation zinc to be brought, carried out the research and development of efficient zinc compound earlier, feed weanling pig with in can substitute or reduce and use common zinc oxide in the diet, and reach the prevention diarrhoea effect identical with high level of oxidation zinc.Nano zine oxide just arises at the historic moment, there are some researches show, can add the 375mg/kg nano zine oxide at feed, remarkable to the growth performance that promotes weanling pig and the effect of preventing to suffer from diarrhoea, can substitute high level of oxidation zinc (3000mg/kg), be a kind of desirable zinc source, but that is that all right is ripe for the nano zine oxide technology, also needs more deep research.
Summary of the invention
For solving the problem of using high level of oxidation zinc to bring in the above-mentioned breeding process, the object of the invention is to provide a kind of rich zinc deactivation lactobacillus micro-ecological preparation, alternative common zinc oxide, fundamentally reduce the use amount of zinc oxide in the weanling pig feed, remove the effectively weaning syndrome of prevention weanling pig appearance, replenish the required zinc element of growth, promote growth, suppress harmful intestinal tract bacteria and prevention diarrhea, because the zinc oxide use amount reduces, can reduce the risk of zinc pollution environment residual in the swine excrement greatly simultaneously.In addition, product of the present invention also can provide efficient zinc source for poultry.
Second purpose of the present invention is to provide a kind of preparation method of rich zinc deactivation lactobacillus micro-ecological preparation.
For achieving the above object, the present invention adopts following technical scheme:
A kind of rich zinc deactivation lactobacillus micro-ecological preparation is made up of the component of following quality proportioning: rich zinc lactobacillus-fermented product 30-60 part, excipient 40-70 part; Wherein rich zinc lactobacillus-fermented product comprises following component: deactivation lactobacillus cell 10 8-10 10Individual/g, zinc element 0.1-1mg/g, culture medium and water.
Described lactic acid bacteria is selected from but is not limited in lactobacillus acidophilus, Lactobacillus salivarius, Lactococcus lactis, bifidobacterium adolescentis, the bifidobacterium infantis one or more.
Described lactobacillus cell is 0.10%-1.20% to the adsorption rate of zinc element.
Described excipient is the mixture of mixture, cornstarch and precipitated silica of 60-100 order maize cob meal and precipitated silica or maize cob meal, cornstarch and precipitated silica three's mixture.
Described zinc element from but be not limited to zinc sulfate, zinc chloride or zinc oxide.
The preparation method of rich zinc deactivation lactobacillus micro-ecological preparation may further comprise the steps:
1) choosing lactobacillus-fermented cultivates;
2) adding mass fraction in fermentation culture is the zinc compound of 0.01%-0.17%;
3) adding mass fraction after the fermentation ends in zymotic fluid is the protective agent of 0.1-0.5%;
4) hot deactivation or cold deactivation, centrifugal collection thalline;
5) thalline and the mixed with excipients that step 4) is obtained, drying, the content that makes residual moisture is less than 15%.
Described step 2) in, before fermentation, zinc compound is added in the nutrient solution, perhaps in the process of fermentation, zinc compound is added in the nutrient solution, perhaps after fermentation ends, zinc compound added and carry out adsorption treatment in the nutrient solution.
Described zinc compound is selected from and is not limited to zinc sulfate, zinc chloride and other soluble zinc salts or zinc oxide.
Described protective agent is the mixture of sodium alginate and glycerine, perhaps skim milk.
The mass ratio of described sodium alginate and glycerine is 1: 1.
Rich zinc deactivation lactobacillus micro-ecological preparation of the present invention, utilize that some have the group of complexing, coordination function on the lactobacillus cell wall, as cell wall polysaccharide and glycoprotein etc., come adsorbing metal ions with metal ion formation ion or covalent bond, with the carrier of lactic acid bacteria as zinc ion, the zinc element is carried near the enteron aisle superficial cell, promote the absorption of enteron aisle to zinc, improve the utilization rate of zinc, thereby satisfy the demand of animal the zinc element; Rich zinc deactivation lactobacillus micro-ecological preparation can effectively be regulated the animal intestinal microecological balance simultaneously, suppresses the pathogenic bacteria of animal intestinal effectively, reaches the purpose of prevention and supplemental treatment animal diarrhoea.Described rich zinc deactivation lactobacillus micro-ecological preparation can reduce the use of zinc oxide, reduces the interpolation level of zinc oxide in the weanling pig daily ration, reduces the irreversible pollution of zinc residual in the pig ight soil to soil.
Case study on implementation
Case study on implementation 1
A kind of rich zinc deactivation lactobacillus acidophilus probiotics, its component are that the rich zinc deactivation of per kilogram lactobacillus acidophilus probiotics contains:
1) rich zinc lactobacillus acidophilus tunning 350 grams, wherein the deactivation lactobacillus acidophilus cells 10 8Individual/g, zinc constituent content 0.57mg/g (lactobacillus acidophilus is 0.16% to the adsorption rate of zinc element), culture medium and water, the adsorption rate computing formula is:
2) specification is that 80 purpose maize cob meals and precipitated silica 650 restrain totally, wherein each 325 gram of maize cob meal and precipitated silica.
Consumption in one ton of complete feed is the 1-2 kilogram, and this component ratio and addition can be used for piglet and culture.
Case study on implementation 2
Case study on implementation 1 described rich zinc deactivation lactobacillus acidophilus probiotics preparation method may further comprise the steps:
1) lactobacillus acidophilus carries out strain preparation earlier, and bacterial screening as minimal medium, adds 0.1%CaCO with solid MRS culture medium 3, the pH value is 6.4.Culture medium is cooled to 37 ℃ through 121 ℃ of sterilizations 20-30 minute, and is standby.
2) fermented and cultured of lactobacillus acidophilus, fermentation medium be with liquid MRS culture medium as minimal medium, the pH value is 6.4, culture medium is cooled to 37 ℃ through 121 ℃ of sterilizations 20-30 minute, adds 0.12% ZnSO 4, mixing, standby.Seed cell age 12-18 hour, inoculum concentration are 3%-5% (V/V or V/W).Fermentation time 36-48 hour, 37 ℃ of fermentation temperatures, the pH value maintains about 5.5.
3) will add the protective agent of mass fraction 0.1-0.5% (w/v) in the zymotic fluid after fermentation ends, described protective agent is a skim milk.
4) deactivation: hot deactivation, zymotic fluid are warming up to 60-80 ℃, keep 30-60 minute, and centrifugal 15 minutes of 3000 rev/mins of zymotic fluids remove supernatant, collect thalline.
5) thalline adds excipient, the rich zinc lactobacillus acidophilus tunning of 350 grams adds excipient 650 grams, and described excipient is the mixture of 80 order cornstarch and precipitated silica, the two ratio 1: 1, mixing was also stirred 20-30 minute, made it fully to mix and adsorb.
6) with the well-mixed rich zinc deactivation lactobacillus acidophilus of excipient in 90 ℃ of-105 ℃ of dryings, make residual moisture≤15%.
Case study on implementation 3
A kind of rich zinc deactivation Lactobacillus salivarius probiotics, its each component are that the rich zinc deactivation of per kilogram Lactobacillus salivarius probiotics contains: 1) rich zinc Lactobacillus salivarius tunning 600 grams, wherein deactivation Lactobacillus salivarius cell content is 10 8Individual/g, zinc constituent content 0.20mg/g (Lactobacillus salivarius is 0.13% to the adsorption rate of zinc element), culture medium and water; 2) specification is 100 purpose maize cob meals and precipitated silica 400 grams, wherein each 200 gram of maize cob meal and precipitated silica.At one ton of consumption of raising the valency material entirely is the 1-2 kilogram, and this component ratio and addition can be used for piglet and culture.
Case study on implementation 4
The preparation method of case study on implementation 3 described rich zinc deactivation Lactobacillus salivarius probioticses may further comprise the steps:
1) Lactobacillus salivarius carries out strain preparation earlier, the bacterial screening culture medium be with solid MRS culture medium as minimal medium, add 0.1%CaCO 3, the pH value is 6.4.121 ℃ of sterilizations of culture medium 20-30 minute are cooled to 37 ℃, and are standby.
2) fermented and cultured of Lactobacillus salivarius, fermentation medium be with liquid MRS culture medium as minimal medium, the pH value is 6.4.121 ℃ of sterilizations of culture medium 20-30 minute are cooled to 37 ℃, and are standby.Seed cell age 12-18 hour, inoculum concentration 3%-5% (V/V or V/W).Fermentation time 36-48 hour, 37 ℃ of fermentation temperatures, the pH value maintains about 5.5.
3) after the fermentation ends, in zymotic fluid, add 0.07%ZnSO 47H 2O kept 2-8 hour, got final product.
4) will add the protective agent of mass fraction 0.1-0.5% (w/v) in the zymotic fluid after fermentation ends, described protective agent is made up of sodium alginate and glycerine, and the mass ratio of the two is 1: 1.
5) 3000 rev/mins of zymotic fluids are centrifugal 15 minutes, remove supernatant, collect thalline.
6) deactivation: freezing deactivation, the thalline of collecting is cooled to about 4 ℃, kept 2-5 hour.
7) thalline adds excipient, and 600 gram Lactobacillus salivarius tunnings add excipient 400 grams, and described excipient is the mixture of 100 order maize cob meals and precipitated silica, and both ratios are 1: 1, mix and stir 20-30 minute, make it fully to mix and absorption.
8) with the well-mixed rich zinc deactivation Lactobacillus salivarius of excipient in 90 ℃ of-105 ℃ of dryings, make residual moisture≤15%.
Case study on implementation 5
A kind of rich zinc deactivation Bifidobacterium probiotics, its each component are that the rich zinc deactivation of per kilogram Bifidobacterium probiotics contains: 1) rich zinc bifidobacterium fermentation product 300 grams, wherein the deactivation bifidobacterium cells 10 9Individual/g, zinc constituent content 0.35mg/g (Bifidobacterium is 0.14% to the adsorption rate of zinc element), culture medium and water; 2) specification is 100 purpose maize cob meals and precipitated silica 700 grams, wherein each 350 gram of maize cob meal and precipitated silica.Consumption at one ton of complete feed is the 1-2 kilogram, and this component ratio and addition can be used for piglet and culture.
Case study on implementation 6
The feed piglet test of embodiment 1 described rich zinc deactivation lactobacillus acidophilus probiotics
(1) test period: 14 days
(2) test combinations: be divided into four groups,
The A group is control group, does not add zinc oxide in the feed and feeds;
B group for the conventional feed nursing (zinc content (in zinc oxide): 〉=2250mg/kg);
Added 2kg/t embodiment 1 described rich zinc deactivation lactobacillus acidophilus probiotics in the C group feed;
D group feed has added the common deactivation lactobacillus acidophilus probiotics of 2kg/t.
(3) subjects: choose 28 ages in days, the figure is close, and 40 of the assorted piglets of the length that is in a good state of health * big binary are divided into four groups.
(4) data are collected and are analyzed: daily ingestion amount, total augment weight, a feedstuff-meat ratio and diarrhoea number etc.
(5) aquaculture management: the feed formula of each group is except zinc oxide addition difference, and other compositions are unanimity.Duration of test is responsible for raising by the special messenger, environmental condition unanimity, free choice feeding, drinking-water.Carry out routine immunization and sterilization, keep the colony house sanitation and hygiene.Observe growing state, carry out relevant record.
(6) result:
Data are summed up:
The rich zinc deactivation of table one lactobacillus acidophilus probiotics is to the growth performance influence of weanling pig
Conclusion:
1. by last Biao Kede, test group C uses rich zinc deactivation lactobacillus acidophilus probiotics, can effectively reduce the zinc oxide addition in the weanling pig feed, even additionally do not adding under the situation of zinc oxide, the growth performance of weanling pig is still unaffected, even better than the control group that adds high level of oxidation zinc (2250mg/kg).The feedstuff-meat ratio of C group reduces by 13.3% than control group A, reduces by 11.5% than high zinc B group, and effect is obvious.
2. in feeding process, C group piglet mental status is good, and feed intake is obviously big than non-oxidation zinc group, has only 1 piglet the diarrhoea situation to occur, and high-level zinc is suitable with adding, and non-oxidation zinc group then has 3.
3.D group is common deactivation lactobacillus acidophilus probiotics group, its feedstuff-meat ratio is the highest in all groups, reaches 1.72, can illustrate, the zinc element is the importance to piglet growth, so should suitably replenish the zinc element in daily ration.Not occurring the diarrhoea phenomenon in the D group, is because the effect of adjustment intestinal microecology balance that lactobacillus micro-ecological preparation plays and prevention diarrhoea.
Case study on implementation 7
Case study on implementation 3 described rich zinc deactivation Lactobacillus salivarius probioticses cooperate interpolation zinc oxide, the piglet of feeding test
(1) test period: 14 days
(2) test combinations: add at the piglet basal diet on the basis of 0.1% rich zinc deactivation Lactobacillus salivarius probiotics, cooperate (except the control group), test its effect with a certain amount of zinc oxide of interpolation.This test is total to four groups of parts, is grouped as follows:
Table two test grouping
Figure BSA00000343185100071
(3) subjects: choose 28 ages in days, the figure is close, the length that the is in a good state of health * assorted piglet of white binary
(4) data are collected and are analyzed: daily ingestion amount, total augment weight, feedstuff-meat ratio etc.
(5) aquaculture management: the feed formula of each group is except zinc oxide addition difference, and other compositions are unanimity.Duration of test is responsible for raising by the special messenger, environmental condition unanimity, free choice feeding, drinking-water.Carry out routine immunization and sterilization, keep the colony house sanitation and hygiene.Observe growing state, carry out relevant record.
(6) result:
Data are summed up:
The rich zinc deactivation of table three Lactobacillus salivarius probiotics is to the growth performance influence of weanling pig
Figure BSA00000343185100081
Conclusion:
1. the result shows, rich zinc deactivation Lactobacillus salivarius probiotics, can be used with zinc oxide, though zinc oxide under the situation of few additive (500mg/kg), the test group piglet growth performance is better than the control group that adds high level of oxidation zinc (2500mg/kg).The feedstuff-meat ratio of test group one, test group two and test group three has reduced by 0.9%, has reduced by 1.8% and reduce by 4.5% than control group respectively.
2. can draw from conclusion 1, rich zinc deactivation Lactobacillus salivarius probiotics is used zinc oxide, both can reduce the addition of zinc oxide in feed, and piglet growth performance is unaffected, can reduce the interpolation cost again, improves raiser's economic benefit.This is one of comparatively desirable using method of rich zinc deactivation lactobacillus micro-ecological preparation of the present invention.
3. the result of test group one, two, three shows, in the piglet basal diet, add on the basis of 0.1% rich zinc deactivation Lactobacillus salivarius probiotics, along with the increase of the addition of zinc oxide, growth performance also is improved, but the addition of lifting amplitude and zinc oxide is disproportionate.
4. through evidence; rich zinc deactivation lactobacillus micro-ecological preparation can be used with zinc oxide; than the better effects if of using zinc oxide separately; can effectively be reduced in the interpolation level (can be low to moderate 500mg/kg) of zinc oxide in the feed; zinc is residual in the minimizing animal wastes, averages out between environmental protection and economic benefit.
Case study on implementation 8
Case study on implementation 1 described rich zinc deactivation lactobacillus acidophilus probiotics feeding dorking
(1) test period: 21 days
(2) test combinations: test is divided into three groups, shared basal diet (zinc content (in zinc oxide): 40mg/kg), add the Different Zinc level respectively,
Control group: the basal diet of feeding;
Test A group: on the basis of basal diet, add 0.1% case study on implementation, 1 described rich zinc deactivation lactobacillus acidophilus probiotics;
Test B group: basal diet (the zinc content (in zinc oxide): 178mg/kg) that has added high zinc;
Test C group: on the basis of basal diet, add 0.1% common deactivation lactobacillus acidophilus probiotics.
(3) test chicken: select 21 ages in days, same batch, individual difference is little, and fryer 180 plumages that health condition is good are equally divided into 3 groups, every group 30 plumage, and individual difference is little between group.
(4) data are collected and are analyzed: feed intake, daily gain and feedstuff-meat ratio.
(5) feeding and management: carry out according to plant's regular job, duration of test is responsible for raising by the special messenger, environmental condition unanimity, free choice feeding, drinking-water.Carry out routine immunization and sterilization, keep the colony house sanitation and hygiene.Observe growing state, carry out relevant record.
(6) result:
Data are summed up:
The rich zinc deactivation of table four Lactobacillus salivarius probiotics is to the growth performance influence of fryer
Figure BSA00000343185100091
Conclusion:
1. in basal diet, be used the growth performance that rich zinc deactivation lactobacillus micro-ecological preparation can improve fryer, feed intake and daily gain comparison you can well imagine high 5.7% and 8.9% according to component, and feedstuff-meat ratio reduces by 3.1%.
2. the zinc content (178mg/kg) of experiment group B is far above test group A (40mg/kg), but growth performance descends, and feed intake and daily gain have reduced by 6.9% and 9.6% respectively, and visible high zinc daily ration has counter productive to the growth performance of fryer.
3. respectively organize the data comparison and can get from above-mentioned, basal diet is used rich zinc deactivation lactobacillus micro-ecological preparation can make the growth performance of fryer be improved, reduce feedstuff-meat ratio, therefore, situation about the growth performance of fryer being had a negative impact at high zinc, in daily ration, suitably replenish some zinc sources, can improve the growth performance of fryer.Product of the present invention is a kind of so efficient zinc source, replenishes zinc can for the growth of poultry, also to regulate the microecological balance of poultry enteron aisle.
Rich zinc inactivated lactobacillus microecological agent of the present invention has following advantage:
1) can effectively prevent and treat the diarrhoea problem that the weanling pig early weaning stress bring, promote the growth of piglet;
2) fundamentally reduce the addition of zinc oxide in the weanling pig feed;
3) effectively reduce the discharge rate of zinc in the animal wastes, reduce the pollution that zinc causes native environment;
4) product of the present invention also can be used for poultry farming, satisfies poultry to the needs of zinc.

Claims (10)

1. a rich zinc deactivation lactobacillus micro-ecological preparation is characterized in that, is made up of the component of following quality proportioning: rich zinc lactobacillus-fermented product 30-60 part, excipient 40-70 part; Wherein rich zinc lactobacillus-fermented product comprises following component: deactivation lactobacillus cell 10 8-10 10Individual/g, zinc element 0.1-1mg/g, culture medium and water.
2. rich zinc deactivation lactobacillus micro-ecological preparation according to claim 1, it is characterized in that: described lactic acid bacteria is selected from one or more in lactobacillus acidophilus, Lactobacillus salivarius, Lactococcus lactis, bifidobacterium adolescentis, the bifidobacterium infantis.
3. rich zinc deactivation lactobacillus micro-ecological preparation according to claim 1, it is characterized in that: described lactobacillus cell is 0.10%-1.20% to the adsorption rate of zinc element.
4. rich zinc deactivation lactobacillus micro-ecological preparation according to claim 1 is characterized in that: described excipient is the mixture of mixture, cornstarch and precipitated silica of 60-100 order maize cob meal and precipitated silica or maize cob meal, cornstarch and precipitated silica three's mixture.
5. rich zinc deactivation lactobacillus micro-ecological preparation according to claim 1, it is characterized in that: described zinc element is from zinc sulfate, zinc chloride and other soluble zinc salts or zinc oxide.
6. rich zinc deactivation lactobacillus micro-ecological preparation preparation method may further comprise the steps:
1) choosing lactobacillus-fermented cultivates;
2) adding mass fraction in fermentation culture is the zinc compound of 0.01%-0.17%;
3) adding mass fraction after the fermentation ends in zymotic fluid is the protective agent of 0.1-0.5%;
4) hot deactivation or cold deactivation, centrifugal collection thalline;
5) thalline and the mixed with excipients that step 4) is obtained, drying makes the content of residual moisture be not more than 15%.
7. the lactobacillus micro-ecological preparation preparation method of enrichment zinc according to claim 6, it is characterized in that: described step 2), before fermentation, zinc compound is added in the nutrient solution, perhaps in the process of fermentation, zinc compound is added in the nutrient solution, perhaps after fermentation ends, zinc compound added and carry out adsorption treatment in the nutrient solution.
8. according to claim 6 or 7 described rich zinc deactivation lactobacillus micro-ecological preparation preparation methods, it is characterized in that: described zinc compound is zinc sulfate, zinc chloride and other soluble zinc salts or zinc oxide.
9. rich zinc deactivation lactobacillus micro-ecological preparation preparation method according to claim 6, it is characterized in that: described protective agent is the mixture of sodium alginate and glycerine, perhaps skim milk.
10. rich zinc deactivation lactobacillus micro-ecological preparation preparation method according to claim 9, it is characterized in that: described: the mass ratio of described sodium alginate and glycerine is 1: 1.
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CN109984260A (en) * 2019-04-12 2019-07-09 生物源生物技术(深圳)股份有限公司 A kind of inactivated lactobacillus microecological agent of Rich in Trace Element and preparation method thereof
CN114574387A (en) * 2022-03-03 2022-06-03 江南大学 High-enrichment organic zinc animal bifidobacterium for promoting growth and reproductive development
CN116491583A (en) * 2023-04-27 2023-07-28 生物源生物技术(深圳)股份有限公司 Microcapsule with gastrointestinal tract regulating function and preparation method and application thereof

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US11382940B2 (en) 2016-08-09 2022-07-12 Cj Cheiljedang Corporation Lactobacillus salivarius CJLS1511, animal feed additive composition comprising same bacterium or dead cells thereof, and method for producing same dead cells
CN109563473A (en) * 2016-08-09 2019-04-02 Cj第制糖株式会社 Lactobacillus salivarius CJLS1511, the method added composition containing the animal feed of the bacterium or dead cell and produce dead cell
CN108841763A (en) * 2018-07-20 2018-11-20 江苏微康生物科技有限公司 A kind of preparation method of zinc dense plants lactobacillus additive
CN109984260A (en) * 2019-04-12 2019-07-09 生物源生物技术(深圳)股份有限公司 A kind of inactivated lactobacillus microecological agent of Rich in Trace Element and preparation method thereof
CN114574387A (en) * 2022-03-03 2022-06-03 江南大学 High-enrichment organic zinc animal bifidobacterium for promoting growth and reproductive development
CN114574387B (en) * 2022-03-03 2023-08-25 江南大学 High-enrichment organic zinc animal bifidobacterium for promoting growth and reproductive development
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