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CN101913960B - Plant nutrition liquid fertilizer and preparation method thereof - Google Patents

Plant nutrition liquid fertilizer and preparation method thereof Download PDF

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Publication number
CN101913960B
CN101913960B CN2010102356099A CN201010235609A CN101913960B CN 101913960 B CN101913960 B CN 101913960B CN 2010102356099 A CN2010102356099 A CN 2010102356099A CN 201010235609 A CN201010235609 A CN 201010235609A CN 101913960 B CN101913960 B CN 101913960B
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concentrated solution
plant
percent
yeast
weight
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CN101913960A (en
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周冠旻
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Guilin Shakes Ecology Agriculture Development Ltd Co
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Abstract

The invention discloses plant nutrition liquid fertilizer and a preparation method thereof. The liquid fertilizer comprises the following components in percentage by weight: 4 to 7 percent of compound amino acid liquid, 2 to 5 percent of ribonucleic acid concentrated solution, 1.5 to 3.5 percent of plant growth hormone concentrated solution, 0.4 to 0.5 percent of non-protein non-amino-acid nitrogen, 2.0 to 5.0 percent of potassium dihydrogen phosphate, 1.5 to 2.5 percent of potassium oxide, 0.1 to 0.25 percent of zinc, 0.4 to 0.6 percent of boron, 0.1 to 0.25 percent of magnesium, 0.1 to 0.25 percent of copper and the balance of water. Compared with the prior art, the product of the invention can gradually regulate the soil acidity or alkalinity of plant rhizospheres, improve microbiological activity, accelerate the decomposition of organic substances, and improve a soil aggregation structure and the utilization rate of the fertilizer due to amino acid, plant growth hormone and ribonucleic acid. The resistance of crops to stress such as low temperature, drought, salt damage, disease, herbicide, phytotoxicity and the like is enhanced after the fertilizer is applied.

Description

One plant nutrition liquid fertilizer and preparation method thereof
Technical field
The present invention relates to a kind of organic fertilizer, be specifically related to plant nutrition liquid fertilizer and preparation method thereof.
Background technology
In agriculture production, adverse environmental factors is usually produced to farm crop and is brought serious threat, and these are referred to as adverse circumstance to the disadvantageous factor of plant-growth.Adverse circumstance generally comprises abiotic stress and biological adverse circumstance.Biological adverse circumstance mainly comprises various diseases and insect pest, and abiotic stress comprises arid, high temperature, low temperature, high salt, toxic and harmful etc.Be the growth indexes that biological adverse circumstance or abiotic stress all can change plant, affect the growth and development process of plant, cause the growth retardation of plant, have a strong impact on the yield and quality of farm crop.
At present, the fertilizers such as the chemical fertilizer of nitrogenous, phosphorus, potassium and small portion animal excrement are mainly used in China farmland.But in order to reach the purpose of volume increase, will repeatedly use every year, and this is the labor force and cause very large burden to the peasant economically.Though the fertilizers such as animal excrement better but originate limitedly, are used in a large number chemical fertilizer and then can be caused soil-geological ground deteriorated even cause the underproduction, in addition, a large amount of productions of chemical fertilizer also can cause the pollution of environment.In order to reduce the pollution to environment, and improve that to use in a large number the soil that chemical fertilizer causes for a long time deteriorated, occur that successively plant-growth is had promoter action, the plant liquid fertilizer of simultaneously organic again environmental protection, be the Chinese invention patent of CN1158830 such as publication number, disclose a kind of natural plant fertilizer, its main component is protein, amino acid, the non-amino acid nitrogen of non-albumen, reducing sugar and dormin, and all the other are water.It is a kind of meta-bolites by Fu Shi grapestone cup fungi CGMCC0289.Its method for making is to make original strain with above-mentioned fungi; Substratum contains wheat bran, sugar, dry orange meal, water, preferably also contains potassium primary phosphate; Technical process comprises the three grade fermemtation cultivation, elimination mycelium and concentrated; Three grades of fermentation times are no more than 14 days altogether.This product volume increase and disease resisting effect are better, to the person poultry harmless, are conducive to soil protection and keep soil fertility, non-environmental-pollution.But the nutrition of this product is complete not, and the anti-adversity of plant improves not obvious after using.
Summary of the invention
It is more complete that the technical problem to be solved in the present invention provides a kind of nutrition, but not only Promoting plant growth, but also can improve plant nutrition liquid fertilizer of plant stress-resistance performance and preparation method thereof.
For solving the problems of the technologies described above, the present invention by the following technical solutions:
One plant nutrition liquid fertilizer, by weight percentage, it consist of:
Moriamin S 4~7%;
Yeast Nucleic Acid concentrated solution 2~5%;
Plant growth hormones concentrated solution 1.5~3.5%;
The non-amino acid nitrogen 4~5% of non-albumen;
Potassium primary phosphate 2.0~5.0%;
Potassium oxide 1.5~2.5%;
Zinc 0.1~0.25%
Boron 0.4~0.6%;
Magnesium 0.1~0.25%;
Copper 0.1~0.25%;
Surplus is water;
Wherein, Moriamin S is made by following methods: get hair, add the hydrochloric acid of 1~6 times of its weight, acidolysis 2~4h under 50~80 ℃ of conditions filters, and collects filtrate, and being concentrated into aminoacids content is 0.8~1.2g/100ml, namely gets Moriamin S;
The Yeast Nucleic Acid concentrated solution is made by following methods: get the distiller's yeast yeast, take waste molasses as cultivating based on normal temperature bottom fermentation 10~15h in the fermentor tank, the sodium hydroxide that adds fermented liquid weight 0.8~1.2% in the gained fermented liquid, be heated to alkaline hydrolysis 1~2h under 70~80 ℃ of conditions, filter, the rna content that filtrate is concentrated into wherein is 10~15%, namely gets the Yeast Nucleic Acid concentrated solution;
The plant growth hormones concentrated solution is made by following methods: get the Plant hormones regulators,gibberellins bacterial classification and cultivate 72~96h through slant strains first, afterwards through shaking table shaking culture 17~48h, then fermentation culture 17~48h in seeding tank, cultivated 7~9 days in fermentation cylinder for fermentation at last, discharge fermenting mixture, filter, filtrate with sulfuric acid acidation to pH=2~3, regulate pH=4.5~5 with yellow soda ash again, it is 200~300 μ g/ml that filtrate after secondary is regulated pH value joint is concentrated into GA content, namely gets the plant growth hormones concentrated solution.
The composition of above-mentioned each component is preferably as follows:
Moriamin S 5~6%;
Yeast Nucleic Acid concentrated solution 3~4%;
Plant growth hormones concentrated solution 2.5~3.0%;
The non-amino acid nitrogen 4~4.5% of non-albumen;
Potassium primary phosphate 3.0~4.0%;
Potassium oxide 1.8~2.3%;
Zinc 0.1~0.2%
Boron 0.45~0.55%;
Magnesium 0.1~0.2%;
Copper 0.1~0.2%;
Surplus is water.
The preparation method of plant nutrition liquid fertilizer of the present invention, its step is as follows:
1) preparation of Moriamin S: get hair, add the hydrochloric acid of 1~6 times of its weight, acidolysis 2~4h under 50~80 ℃ of conditions filters, and collects filtrate, and being concentrated into aminoacids content is 0.8~1.2g/100ml, namely gets Moriamin S;
2) preparation of Yeast Nucleic Acid concentrated solution: get the distiller's yeast yeast, take waste molasses as cultivating based on normal temperature bottom fermentation 10~15h in the fermentor tank, the sodium hydroxide that adds fermented liquid weight 0.8~1.2% in the gained fermented liquid, be heated to alkaline hydrolysis 1~2h under 70~80 ℃ of conditions, filter, the rna content that filtrate is concentrated into wherein is 10~15%, namely gets the Yeast Nucleic Acid concentrated solution;
3) preparation of plant growth hormones concentrated solution: get the Plant hormones regulators,gibberellins bacterial classification and cultivate 72~96h through slant strains first, afterwards through shaking table shaking culture 17~48h, then fermentation culture 17~48h in seeding tank, cultivated 7~9 days in fermentation cylinder for fermentation at last, discharge fermenting mixture, filter, filtrate with sulfuric acid acidation to pH=2~3, regulate pH=4.5~5 with yellow soda ash again, it is 200~300 μ g/ml that filtrate after secondary is regulated the pH value is concentrated into GA content, namely gets the plant growth hormones concentrated solution;
4) take by weighing the above-mentioned Moriamin S that makes, Yeast Nucleic Acid concentrated solution and plant growth hormones concentrated solution by proportional quantity, the non-amino acid nitrogen of non-albumen, potassium primary phosphate, potassium oxide, zinc, boron, magnesium, the Tong Heshui that add again proportional quantity, mix, and get final product.
Among the above-mentioned preparation method,
Step 2) in, the consumption proportion of waste molasses and distiller's yeast yeast is 100~150: 1.
Step 3) in, the temperature of Plant hormones regulators,gibberellins cultivations at different levels and used substratum are identical with the condition of cellar culture Plant hormones regulators,gibberellins, specifically can be: the temperature of cultivations at different levels is 25~33 ℃.The substratum of spawn culture is the PDA substratum, and the substratum of shaking table shaking culture is to remove agar in the PDA substratum, and shaking speed is 150~180r/min; The substratum of seeding tank and fermentor cultivation is Semen Maydis powder, wheat bran and water, wherein Semen Maydis powder: wheat bran: the weight ratio of water=7: 3: 10, air flow are 0.1~0.2VVM; The access amount of bacterial classification is the ratio of 5% (volume) in the cultivations at different levels.
Plant nutrition liquid fertilizer of the present invention needs product is used after by 100~400 times of dilutions in use.
Compared with prior art, the advantage of plant nutrition liquid fertilizer of the present invention is:
1, not only contains amino acid, plant growth hormones and trace element in the product, but also contain Yeast Nucleic Acid, progressively regulating plant rhizosphere soil potential of hydrogen improves microorganism active, accelerates organic matter decomposition, improves soil aggregate, improves utilization rate of fertilizer; Also can promote plant root system development, particularly owing to the receptivity that strengthens blade face, root has strengthened the receptivity of plant to nutrition;
2, the amino acid in the product, plant growth hormones and Yeast Nucleic Acid all adopt zymotechnique to make, more environmental protection;
3, use the nutrient liquid fertilizer that makes with said ratio after, obvious pest effect is arranged, obviously reduce even do not have such as insects such as red spider, aphid, bollworm, Liriomyzas; And plant obviously strengthens the resistivity of the adverse circumstances such as low temperature, arid, salt damage, disease, weedicide and poisoning.
Embodiment
The invention will be further described with embodiment for the below.
Embodiment 1
One, plant nutrition liquid fertilizer, each component is composed as follows by weight percentage:
Aminoacids content is the Moriamin S 5% of 1.0g/100ml;
Rna content is the Yeast Nucleic Acid concentrated solution 2.5% of 15% (weight);
GA content is the plant growth hormones concentrated solution 2.5% of 200 μ g/ml;
The non-amino acid nitrogen 5% of non-albumen;
Potassium primary phosphate 2.5%;
Potassium oxide 2.0%;
Zinc 0.1%
Boron 0.5%;
Magnesium 0.1%;
Copper 0.1%;
Surplus is water.
Two, the preparation method is as follows:
1) preparation of Moriamin S: get hair, add the hydrochloric acid of 3 times of its weight, acidolysis 4h under 50 ℃ of conditions filters, and collects filtrate, and being concentrated into aminoacids content is 1.0g/100ml, namely gets Moriamin S;
2) preparation of Yeast Nucleic Acid concentrated solution: get the distiller's yeast yeast, take waste molasses as cultivating based on normal temperature bottom fermentation 11h in the fermentor tank, the sodium hydroxide that adds fermented liquid weight 0.8% in the gained fermented liquid, be heated to alkaline hydrolysis 2h under 80 ℃ of conditions, filter, the rna content that filtrate is concentrated into wherein is 15% (weight), namely gets the Yeast Nucleic Acid concentrated solution; Wherein, the weight proportion of waste molasses and distiller's yeast yeast is 120: 1;
3) preparation of plant growth hormones concentrated solution: get the Plant hormones regulators,gibberellins bacterial classification and cultivate 90h through slant strains first, afterwards through shaking table shaking culture 36h, then fermentation culture 36h in seeding tank, cultivated 8 days in fermentation cylinder for fermentation at last, discharge fermenting mixture, filter, filtrate with sulfuric acid acidation to pH=2.5, regulate pH=4.8 with yellow soda ash again, it is 200 μ g/ml that the filtrate after secondary is regulated the pH value is concentrated into GA content, namely gets the plant growth hormones concentrated solution; Wherein the substratum of spawn culture is PDA substratum (potato 200g, glucose 20g, agar 20g, water 1000ml), the substratum of shaking table shaking culture is to remove agar (potato 200g, glucose 20g, water 1000ml) in the PDA substratum, and shaking speed is 180r/min; The substratum of seeding tank and fermentor cultivation is Semen Maydis powder, wheat bran and water, wherein Semen Maydis powder: wheat bran: the weight ratio of water=7: 3: 10, air flow are 0.2VVM; The access amount of bacterial classification is the ratio of 5% (volume) in the cultivations at different levels; The temperature of cultivations at different levels is 25~29 ℃;
4) take by weighing the above-mentioned Moriamin S that makes, Yeast Nucleic Acid concentrated solution and plant growth hormones concentrated solution by proportional quantity, the non-amino acid nitrogen of non-albumen, potassium primary phosphate, potassium oxide, zinc, boron, magnesium, the Tong Heshui that add again proportional quantity, mix, and get final product.
With 150 times of the above-mentioned product dilute with waters that makes, spray paddy rice, two kinds of crops of wheat, compare every mu of volume increase 20~30% with the paddy rice that does not spray chemical fertilizer, wheat crop.
Embodiment 2
One, plant nutrition liquid fertilizer, each component is composed as follows by weight percentage:
Aminoacids content is the Moriamin S 7% of 0.8g/100ml;
Rna content is the Yeast Nucleic Acid concentrated solution 3% of 10% (weight);
GA content is the plant growth hormones concentrated solution 3% of 300 μ g/ml;
The non-amino acid nitrogen 4% of non-albumen;
Potassium primary phosphate 3.0%;
Potassium oxide 1.5%;
Zinc 0.25%
Boron 0.5%;
Magnesium 0.2%;
Copper 0.1%;
Surplus is water.
Two, the preparation method is as follows:
1) preparation of Moriamin S: get hair, add the hydrochloric acid of 5 times of its weight, acidolysis 3h under 70 ℃ of conditions filters, and collects filtrate, and being concentrated into aminoacids content is 0.8g/100ml, namely gets Moriamin S;
2) preparation of Yeast Nucleic Acid concentrated solution: get the distiller's yeast yeast, take waste molasses as cultivating based on normal temperature bottom fermentation 15h in the fermentor tank, the sodium hydroxide that adds fermented liquid weight 1% in the gained fermented liquid, be heated to alkaline hydrolysis 1h under 75 ℃ of conditions, filter, the rna content that filtrate is concentrated into wherein is 10% (weight), namely gets the Yeast Nucleic Acid concentrated solution; Wherein, the weight proportion of waste molasses and distiller's yeast yeast is 150: 1;
3) preparation of plant growth hormones concentrated solution: get the Plant hormones regulators,gibberellins bacterial classification and cultivate 72h through slant strains first, afterwards through shaking table shaking culture 48h, then fermentation culture 24h in seeding tank, cultivated 9 days in fermentation cylinder for fermentation at last, discharge fermenting mixture, filter, filtrate with sulfuric acid acidation to pH=2.8, regulate pH=5.0 with yellow soda ash again, it is 300 μ g/ml that the filtrate after secondary is regulated the adjusting of pH value is concentrated into GA content, namely gets the plant growth hormones concentrated solution; Wherein the substratum of spawn culture is PDA substratum (potato 200g, glucose 20g, agar 20g, water 1000ml), the substratum of shaking table shaking culture is to remove agar (potato 200g, glucose 20g, water 1000ml) in the PDA substratum, and shaking speed is 150r/min; The substratum of seeding tank and fermentor cultivation is Semen Maydis powder, wheat bran and water, wherein starch: sugar: the weight ratio of cake powder=7: 3: 10, air flow are 0.12VVM; The access amount of bacterial classification is the ratio of 5% (volume) in the cultivations at different levels; The temperature of cultivations at different levels is 25~33 ℃;
4) take by weighing the above-mentioned Moriamin S that makes, Yeast Nucleic Acid concentrated solution and plant growth hormones concentrated solution by proportional quantity, the non-amino acid nitrogen of non-albumen, potassium primary phosphate, potassium oxide, zinc, boron, magnesium, the Tong Heshui that add again proportional quantity, mix, and get final product.
With 250 times of the above-mentioned product dilute with waters that makes, spray string bean, cucumber and three kinds of vegetables of eggplant, compare every mu of volume increase 25~38% with the string bean that does not spray chemical fertilizer, cucumber and eggplant.Wherein, the red spider, the hang spring worm that spray the experiment Tanaka of string bean behind this product, cucumber and three kinds of vegetables of eggplant obviously reduce, and in the week after spraying this product, above-mentioned insect does not all have.
Embodiment 3
One, plant nutrition liquid fertilizer, each component is composed as follows by weight percentage:
Aminoacids content is the Moriamin S 4% of 1.2g/100ml;
Rna content is the Yeast Nucleic Acid concentrated solution 4% of 15% (weight);
GA content is the plant growth hormones concentrated solution 3.5% of 250 μ g/ml;
The non-amino acid nitrogen 4.5% of non-albumen;
Potassium primary phosphate 2.0%;
Potassium oxide 2.5%;
Zinc 0.15%
Boron 0.6%;
Magnesium 0.25%;
Copper 0.2%;
Surplus is water.
Two, the preparation method is as follows:
1) preparation of Moriamin S: get hair, add the hydrochloric acid of 2 times of its weight, acidolysis 4h under 80 ℃ of conditions filters, and collects filtrate, and being concentrated into aminoacids content is 1.2g/100ml, namely gets Moriamin S;
2) preparation of Yeast Nucleic Acid concentrated solution: get the distiller's yeast yeast, take waste molasses as cultivating based on normal temperature bottom fermentation 12h in the fermentor tank, the sodium hydroxide that adds fermented liquid weight 1.2% in the gained fermented liquid, be heated to alkaline hydrolysis 1.5h under 70 ℃ of conditions, filter, the rna content that filtrate is concentrated into wherein is 15% (weight), namely gets the Yeast Nucleic Acid concentrated solution; Wherein, the weight proportion of waste molasses and distiller's yeast yeast is 100: 1;
3) preparation of plant growth hormones concentrated solution: get the Plant hormones regulators,gibberellins bacterial classification and cultivate 96h through slant strains first, afterwards through shaking table shaking culture 18h, then fermentation culture 48h in seeding tank, cultivated 7 days in fermentation cylinder for fermentation at last, discharge fermenting mixture, filter, filtrate with sulfuric acid acidation to pH=2.0, regulate pH=4.5 with yellow soda ash again, it is 250 μ g/ml that the filtrate after secondary is regulated the pH value is concentrated into GA content, namely gets the plant growth hormones concentrated solution; Wherein the substratum of spawn culture is PDA substratum (potato 200g, glucose 20g, agar 20g, water 1000ml), the substratum of shaking table shaking culture is to remove agar (potato 200g, glucose 20g, water 1000ml) in the PDA substratum, and shaking speed is 150r/min; The substratum of seeding tank and fermentor cultivation is Semen Maydis powder, wheat bran and water, wherein Semen Maydis powder: wheat bran: the weight ratio of water=7: 3: 10, air flow are 0.15VVM; The access amount of bacterial classification is the ratio of 5% (volume) in the cultivations at different levels; The temperature of cultivations at different levels is 28~31 ℃;
4) take by weighing the above-mentioned Moriamin S that makes, Yeast Nucleic Acid concentrated solution and plant growth hormones concentrated solution by proportional quantity, the non-amino acid nitrogen of non-albumen, potassium primary phosphate, potassium oxide, zinc, boron, magnesium, the Tong Heshui that add again proportional quantity, mix, and get final product.
With 350 times of the above-mentioned product dilute with waters that makes, spray watermelon, two kinds of crops of strawberry, aphid, red spider obviously reduce, and compare every mu of volume increase 30~35% with the watermelon that does not spray chemical fertilizer, strawberry crop.
Embodiment 4
One, plant nutrition liquid fertilizer, each component is composed as follows by weight percentage:
Aminoacids content is the Moriamin S 6% of 1.0g/100ml;
Rna content is the Yeast Nucleic Acid concentrated solution 5% of 12% (weight);
GA content is the plant growth hormones concentrated solution 1.5% of 300 μ g/ml;
The non-amino acid nitrogen 5% of non-albumen;
Potassium primary phosphate 5%;
Potassium oxide 1.8%;
Zinc 0.2%
Boron 0.4%;
Magnesium 0.15%;
Copper 0.25%;
Surplus is water.
Two, the preparation method is with embodiment 3.

Claims (5)

1. a plant nutrition liquid fertilizer is characterized in that: by weight percentage, and it consist of:
Moriamin S 4~7%;
Yeast Nucleic Acid concentrated solution 2~5%;
Plant growth hormones concentrated solution 1.5~3.5%;
The non-amino acid nitrogen 4~5% of non-albumen;
Potassium primary phosphate 2.0~5.0%;
Potassium oxide 1.5~2.5%;
Zinc 0.1~0.25%
Boron 0.4~0.6%;
Magnesium 0.1~0.25%;
Copper 0.1~0.25%;
Surplus is water;
Wherein, Moriamin S is made by following methods: get hair, add the hydrochloric acid of 1~6 times of its weight, acidolysis 2~4h under 50~80 ℃ of conditions filters, and collects filtrate, and being concentrated into aminoacids content is 0.8~1.2g/100ml, namely gets Moriamin S;
The Yeast Nucleic Acid concentrated solution is made by following methods: get the distiller's yeast yeast, take waste molasses as cultivating based on normal temperature bottom fermentation 10~15h in the fermentor tank, the sodium hydroxide that adds fermented liquid weight 0.8~1.2% in the gained fermented liquid, be heated to alkaline hydrolysis 1~2h under 70~80 ℃ of conditions, filter, the rna content that filtrate is concentrated into wherein is 10~15% weight, namely gets the Yeast Nucleic Acid concentrated solution;
The plant growth hormones concentrated solution is made by following methods: get the Plant hormones regulators,gibberellins bacterial classification and cultivate 72~96h through slant strains first, afterwards through shaking table shaking culture 17~48h, then fermentation culture 17~48h in seeding tank, cultivated 7~9 days in fermentation cylinder for fermentation at last, discharge fermenting mixture, filter, filtrate with sulfuric acid acidation to pH=2~3, regulate pH=4.5~5 with yellow soda ash again, it is 200~300 μ g/ml that filtrate after secondary is regulated the pH value is concentrated into GA content, namely gets the plant growth hormones concentrated solution.
2. plant nutrition liquid fertilizer according to claim 1 is characterized in that: each component composed as follows:
Moriamin S 5~6%;
Yeast Nucleic Acid concentrated solution 3~4%;
Plant growth hormones concentrated solution 2.5~3.0%;
The non-amino acid nitrogen 4~4.5% of non-albumen;
Potassium primary phosphate 3.0~4.0%;
Potassium oxide 1.8~2.3%;
Zinc 0.1~0.2%
Boron 0.45~0.55%;
Magnesium 0.1~0.2%;
Copper 0.1~0.2%;
Surplus is water.
3. the preparation method of plant nutrition liquid fertilizer claimed in claim 1 is characterized in that step is as follows:
1) preparation of Moriamin S: get hair, add the hydrochloric acid of 1~6 times of its weight, acidolysis 2~4h under 50~80 ℃ of conditions filters, and collects filtrate, and being concentrated into aminoacids content is 0.8~1.2g/100ml, namely gets Moriamin S;
2) preparation of Yeast Nucleic Acid concentrated solution: get the distiller's yeast yeast, take waste molasses as cultivating based on normal temperature bottom fermentation 10~15h in the fermentor tank, the sodium hydroxide that adds fermented liquid weight 0.8~1.2% in the gained fermented liquid, be heated to alkaline hydrolysis 1~2h under 70~80 ℃ of conditions, filter, the rna content that filtrate is concentrated into wherein is 10~15% weight, namely gets the Yeast Nucleic Acid concentrated solution;
3) preparation of plant growth hormones concentrated solution: get the Plant hormones regulators,gibberellins bacterial classification and cultivate 72~96h through slant strains first, afterwards through shaking table shaking culture 17~48h, then fermentation culture 17~48h in seeding tank, cultivated 7~9 days in fermentation cylinder for fermentation at last, discharge fermenting mixture, filter, filtrate with sulfuric acid acidation to pH=2~3, regulate pH=4.5~5 with yellow soda ash again, it is 200~300 μ g/ml that filtrate after secondary is regulated the pH value is concentrated into GA content, namely gets the plant growth hormones concentrated solution;
4) take by weighing the above-mentioned Moriamin S that makes, Yeast Nucleic Acid concentrated solution and plant growth hormones concentrated solution by proportional quantity, the non-amino acid nitrogen of non-albumen, potassium primary phosphate, potassium oxide, zinc, boron, magnesium, the Tong Heshui that add again proportional quantity, mix, and get final product.
4. the preparation method of plant nutrition liquid fertilizer according to claim 3, it is characterized in that: in the step 3), the temperature of cultivations at different levels is 25~33 ℃.
5. the preparation method of plant nutrition liquid fertilizer according to claim 3, it is characterized in that: in the step 3), the substratum of spawn culture is the PDA substratum, and the substratum of shaking table shaking culture is to remove agar in the PDA substratum, and shaking speed is 150~180r/min; The substratum of seeding tank and fermentor cultivation is Semen Maydis powder, wheat bran and water, wherein Semen Maydis powder: wheat bran: the weight ratio=7:3:10 of water; The access amount of bacterial classification is the ratio of 5% volume in the cultivations at different levels.
CN2010102356099A 2010-07-24 2010-07-24 Plant nutrition liquid fertilizer and preparation method thereof Expired - Fee Related CN101913960B (en)

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