CN101830999A - Method for extracting platycodon root polysaccharide - Google Patents
Method for extracting platycodon root polysaccharide Download PDFInfo
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- CN101830999A CN101830999A CN201010178466A CN201010178466A CN101830999A CN 101830999 A CN101830999 A CN 101830999A CN 201010178466 A CN201010178466 A CN 201010178466A CN 201010178466 A CN201010178466 A CN 201010178466A CN 101830999 A CN101830999 A CN 101830999A
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- platycodon root
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Abstract
The invention discloses a method for extracting platycodon root polysaccharide, which relates to a method for preparing the platycodon root polysaccharide and solves the problems of low yield, long consumed time, low polysaccharide activity and fussy process in the conventional platycodon root polysaccharide extraction process. The method for extracting the platycodon root polysaccharide comprises the following steps of: selecting dry platycodon root, degreasing the crushed powder, then extracting polysaccharide from the platycodon root by a microwave-aid water extracting method to obtain a polysaccharide extract, removing protein and then separating the extract by using an ultra-speed centrifuge, concentrating the extract by using a film under vacuum, and freeze-drying the extract by using a vacuum freeze dryer after dialysis concentration to obtain platycodon root polysaccharide freeze-drying powder. The platycodon root polysaccharide extracted by the method has the effects of enhancing immunologic function, resisting tumor, reducing blood fat and blood pressure, resisting cruor and thrombus, losing weight, caring skin, promoting the healing of cornea wound, improving dry eyes and the like. The platycodon root polysaccharide can be applied in the fields of food, health-care products, cosmetics, medicaments, biochemical engineering and the like.
Description
Technical field
The present invention relates to a kind of preparation method of platycodon root polysaccharide.
Background technology
Traditional platycodon root polysaccharide extracting method is based on the water-bath extraction, and this method is consuming time, consumption power, ethanol waste are big, and the extraction yield of polysaccharide is low, cost is high.Also useful at present ultrasonic wave, supercritical extraction technique, microwave assisting method and enzyme process extract polysaccharide.Though ultrasonic extraction can shorten extraction time greatly, increase work efficiency, save solvent, because hyperacoustic cavitation has destruction to the macromole sugar chain, and the polysaccharide molecular weight of extraction is reduced, destroyed original polysaccharide structures; And supercritical extraction technique can not be widely used again in a large amount of leaching process, as uses entrainment agent, and solvent load is also not little, as CO
2Can not reclaim, also be a very big waste.Enzymatic Extraction then requires the extraction conditions strictness, otherwise the easy inactivation of enzyme, the difficult control of operating process.Compare preceding several method, it is strong that the microwave rule has penetration power, heating fast and evenly can significantly be shortened extraction time, improves the extraction yield of polysaccharide, so the present invention adopts microwave-assisted to extract, convenient, practicality, extraction cost is cheap, and utilize the response surface analysis method to optimize extraction conditions, for industrial extensive extraction Platycodon grandiflouorum polysaccharide is provided fundamental basis and the data support.
Summary of the invention
Low in order to solve the platycodon root polysaccharide extraction yield, active not high problem the invention provides the method that a kind of microwave method prepares platycodon root polysaccharide, can effectively improve the extraction yield and the product activity of platycodon root polysaccharide.Among the present invention, a kind of extracting method of platycodon root polysaccharide, the step that it is characterized in that the platycodon root polysaccharide extracting method is as follows: one, choose exsiccant balloonflower root rhizome, pulverized 60 mesh sieves, the ratio that with the solid-liquid ratio is 1g: 20mL～1g: 60mL adds the Platycodon Root and the aqueous solution respectively, after 160～800W microwave power acts on 1～4min down behind the immersion 1h, 40～70 ℃ of water-bath 60min obtain being rich in the extraction liquid of platycodon root polysaccharide.Carry out solid-liquid separation with the horizontal helical type whizzer with the speed of 1500～2000r/min, isolate extracting solution and residue; Two, with sevag method (chloroform: deproteinated 2～5 times repeatedly propyl carbinol=4: 1), dialysis 24～48h, 45～55 ℃ of vacuum gyroscope concentrated extracting solutions; Three, with the dehydrated alcohol that in extracting solution, adds 3～5 times of volumes after the phenolsulfuric acid method mensuration solution absorbance value; Four, pure hypostasis is separated, obtain the platycodon root polysaccharide powder with vacuum-freeze-dry machine vacuum lyophilization under 0.4～0.7mbar, temperature-40～-50 ℃ condition.
The present invention is in the platycodon root polysaccharide extracting method, adopt the method for microwave auxiliary extraction, make platycodon root polysaccharide be able to abundant release, make the extraction yield of polysaccharide can reach 20～30%, exceed 15～20% than common water-bath extraction yield, improved the economic worth of platycodon root polysaccharide greatly.Technology of the present invention is simple, extraction yield height, suitable promotion and application.
Embodiment
Embodiment one: in this embodiment, a kind of extracting method of platycodon root polysaccharide, the step that it is characterized in that the method that platycodon root polysaccharide extracts is as follows: one, choose exsiccant balloonflower root rhizome, pulverized 60 mesh sieves, with certain solid-liquid ratio is that the ratio of 1g: 20mL～1g: 60mL adds the Platycodon Root and the aqueous solution respectively, soak behind the 1h extract down with certain microwave power after, 40～70 ℃ of water-baths obtain being rich in the extraction liquid of platycodon root polysaccharide.Carry out solid-liquid separation with the horizontal helical type whizzer with the speed of 1500～2000r/min, isolate extracting solution and residue; Two, with the sevag method (chloroform: deproteinated repeatedly propyl carbinol=4: 1), dialysis tubing is dialysed, 45～55 ℃ of vacuum gyroscope concentrated extracting solutions; Three, with in extracting solution, adding times volume of ethanol solution after the phenolsulfuric acid method mensuration solution absorbance value; Four, pure hypostasis is separated, obtain the platycodon root polysaccharide powder with vacuum-freeze-dry machine vacuum lyophilization under 0.4～0.7mbar, temperature-40～-50 ℃ condition.
Embodiment two: what this embodiment and embodiment one were different is: microwave power 300～500W effect 1～4min down in the step 1.Other is identical with embodiment one.
Embodiment three: what this embodiment and embodiment one were different is: the water-bath time is 40～60min in the step 1.Centrifugation time is 10～20min.Other is identical with embodiment one.
Embodiment four: what this embodiment and embodiment one were different is: (the chloroform: deproteinated 2～5 times repeatedly propyl carbinol=4: 1), dialysis 24～48h of sevag method in the step 2.
Embodiment five: what this embodiment and embodiment one were different is: the dehydrated alcohol that adds 3～5 times of volumes in the step 3 in the polysaccharide extraction liquid.
Embodiment six: what this embodiment and embodiment one were different is: the vacuum-freeze-dry time is 24～48 hours in the step 4.
Claims (6)
1. the extracting method of a platycodon root polysaccharide, the step that it is characterized in that the platycodon root polysaccharide extracting method is as follows: one, choose exsiccant balloonflower root rhizome, pulverized 60 mesh sieves, with certain solid-liquid ratio is that the ratio of 1g: 20mL～1g: 60mL adds the Platycodon Root and the aqueous solution respectively, soak behind the 1h extract down with certain microwave power after, 40～70 ℃ of water-baths obtain being rich in the extraction liquid of platycodon root polysaccharide.Carry out solid-liquid separation with the horizontal helical type whizzer with the speed of 1500～2000r/min, isolate extracting solution and residue; Two, with the sevag method (chloroform: deproteinated repeatedly propyl carbinol=4: 1), dialysis tubing is dialysed, 45～55 ℃ of vacuum gyroscope concentrated extracting solutions; Three, with in extracting solution, adding times volume of ethanol solution after the phenolsulfuric acid method mensuration solution absorbance value; Four, pure hypostasis is separated, obtain the platycodon root polysaccharide powder with vacuum-freeze-dry machine vacuum lyophilization under 0.4～0.7mbar, temperature-40～-50 ℃ condition.
2. the extracting method of a kind of platycodon root polysaccharide according to claim 1 is characterized in that microwave power 300～500W acts on 1～4min down in the step 1.
3. the extracting method of a kind of platycodon root polysaccharide according to claim 1 is characterized in that the water-bath time is 40～60min in the step 1.Centrifugation time is 10～20min.
4. the extracting method of a kind of platycodon root polysaccharide according to claim 1 is characterized in that the (chloroform: deproteinated 2～5 times repeatedly propyl carbinol=4: 1), dialysis 24～48h of sevag method in the step 2.
5. the extracting method of a kind of platycodon root polysaccharide according to claim 1 is characterized in that adding in the polysaccharide extraction liquid in the step 3 dehydrated alcohol of 3～5 times of volumes.
6. a kind of cervus elaphus linnaeus extraction method of polysaccharides according to claim 1 is characterized in that the vacuum-freeze-dry time is 24～48 hours in the step 4.
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| CN201010178466A CN101830999A (en) | 2010-05-21 | 2010-05-21 | Method for extracting platycodon root polysaccharide |
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| CN201010178466A CN101830999A (en) | 2010-05-21 | 2010-05-21 | Method for extracting platycodon root polysaccharide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102477103A (en) * | 2010-11-22 | 2012-05-30 | 中国科学院上海药物研究所 | Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof |
| CN102627702A (en) * | 2012-04-24 | 2012-08-08 | 哈尔滨工业大学 | Extraction method of squid hyaluronic acid |
| CN104292355A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Method for extraction of platycodon grandiflorum polysaccharide |
| CN106690244A (en) * | 2015-11-14 | 2017-05-24 | 黄志萍 | Preparation method and application of novel sweet Gynostemma pentaphyllum Makino. sweetener |
| CN108690145A (en) * | 2018-08-07 | 2018-10-23 | 浙江师范大学 | The microwave auxiliary extracting method of Hawthorn Polysaccharides |
| CN114478821A (en) * | 2022-03-01 | 2022-05-13 | 安徽中医药大学 | Platycodon grandiflorum polysaccharide capable of improving body constitution of mice induced by high-fat diet and application of platycodon grandiflorum polysaccharide |
| CN116836310A (en) * | 2023-06-28 | 2023-10-03 | 天津商业大学 | Radix Platycodi polysaccharide with blood sugar reducing effect and preparation method thereof |
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| CN101104645A (en) * | 2007-06-22 | 2008-01-16 | 山东理工大学 | A process for extracting platycodon polysaccharides assisted by ultrasonic waves |
| CN101445567A (en) * | 2008-12-19 | 2009-06-03 | 山东理工大学 | Method for extracting platycodon polysaccharides with pressurized hot water |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101104645A (en) * | 2007-06-22 | 2008-01-16 | 山东理工大学 | A process for extracting platycodon polysaccharides assisted by ultrasonic waves |
| CN101445567A (en) * | 2008-12-19 | 2009-06-03 | 山东理工大学 | Method for extracting platycodon polysaccharides with pressurized hot water |
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| LI LEI等: ""Optimization of microwave-assisted extraction of polysaccharides in the flower of Platycodon grandiflorum by response surface"", 《INT J AGRIC & BIOL ENG》 * |
| 吴彦: ""桔梗中多糖的提取及测定"", 《安庆师范学院学报（自然科学版）》 * |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102477103A (en) * | 2010-11-22 | 2012-05-30 | 中国科学院上海药物研究所 | Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof |
| CN102477103B (en) * | 2010-11-22 | 2014-09-10 | 中国科学院上海药物研究所 | Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof |
| CN102627702A (en) * | 2012-04-24 | 2012-08-08 | 哈尔滨工业大学 | Extraction method of squid hyaluronic acid |
| CN104292355A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Method for extraction of platycodon grandiflorum polysaccharide |
| CN106690244A (en) * | 2015-11-14 | 2017-05-24 | 黄志萍 | Preparation method and application of novel sweet Gynostemma pentaphyllum Makino. sweetener |
| CN106690244B (en) * | 2015-11-14 | 2019-12-24 | 黄志萍 | Preparation and application of novel sweet gynostemma pentaphylla sweetener |
| CN108690145A (en) * | 2018-08-07 | 2018-10-23 | 浙江师范大学 | The microwave auxiliary extracting method of Hawthorn Polysaccharides |
| CN114478821A (en) * | 2022-03-01 | 2022-05-13 | 安徽中医药大学 | Platycodon grandiflorum polysaccharide capable of improving body constitution of mice induced by high-fat diet and application of platycodon grandiflorum polysaccharide |
| CN116836310A (en) * | 2023-06-28 | 2023-10-03 | 天津商业大学 | Radix Platycodi polysaccharide with blood sugar reducing effect and preparation method thereof |
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Application publication date: 20100915 |