CN101828641A - Animal feed using lycopene fungus oil as additive - Google Patents
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Abstract
The invention belongs to the technical fields of waste recycling and environmental protection, in particular to an animal feed using lycopene fungus oil as an additive. The animal feed comprises 98.5 portions of a basal feed by weight and 1.5 portions of the lycopene fungus oil by weight, wherein the lycopene fungus oil is the fungus oil containing lycopene, which is produced after lycopene is abstracted and crystallized in the process of producing the lycopene by fermenting with microorganisms. The invention recycles the lycopene fungus oil, enhances the bioavailability and can not produce environmental pollution caused by emissions. The lycopene fungus oil replaces the prior lycopene feed additive and reduces the feed cost. The prepared feed is safe and reliable, and can enhance the animal immunologic function and reduce the morbidity.
Description
Technical field
The invention belongs to refuse reclamation and environmental protection technical field, specifically relate to a kind of animal feed that utilizes lycopene fungus oil as additive.
Background technology
Natural Beta-carotene is as vitamins nutrition fortifier and colouring agent, be widely used in food by The World Health Organization (WHO) and international food and agricultural organization (FAO) approval as the auxotype additive, health products and aquaculture. the carrotene that is widely used in aquaculture at present mainly is beta carotene, astaxanthin and lutein etc.Lycopene is a kind of in more than the 600 kind of carotenoid, owing to do not possess the activity of provitamin A, the past is not much accounted of always.Recent international research institution studies show that: 11 conjugated double bonds that lycopene had, be to have the maximum straight chain formula carrotene of conjugated double bond in the carotenoid, its oxidation resistance is 2 times of beta carotene, be 10 times of vitamin E, make it become a kind of carrotene that has oxidation resistance most, can suppress the generation of biological interior free yl effectively, improve the immunity of animal, the viability of livestock and poultry, promote the growth of animal; Lycopene can also effectively increase hair color, yellowish pink, the egg look of poultry as a kind of natural colorant in addition.Lycopene had been used to culture already the extremely concern of countries in the world as a kind of novel, high nutrition, natural feed addictive.Transnational major company as many countries such as Japanese KAGOME company, Switzerland ROCHE companies all begins one's study, develops the feed that is rich in lycopene, and preceding Russia just utilizes biofermentation production to be rich in the lycopene thalline as feed addictive.
Continuous development along with microbial technique, the technology that the employing microbe fermentation method prepares lycopene is more and more ripe, microbial fermentation prepares in the lycopene process, after refinement, crystalline lycopene, the direct discharging of these bacterium oil tends to produce a large amount of bacterium oil that contains lycopene, if will cause great pollution to environment.Find after deliberation: except that containing 1～2% lycopene, also contain multiple other carotenoid, in the lycopene fungus oil as canthaxanthin, beta carotene, β-kryptoxanthin, δ-carrotene or the like.And tomato bacterium main body of oil is the grease that bacterial metabolism produces, wherein linoleic plus oleic acid just accounts for more than 60%, palmitic acid accounts for 30%, contain a spot of palmitoleic acid, myristic acid, leukotrienes, arachidic acid etc. in addition, these all are the aliphatic acid of necessary C16 of animal growth and C18 class.And health ministry Food Hygiene Surveillance check institute proves avirulent to the virulence test of this strain culture, and testing result shows that it is safe and reliable developing this bacterium oil.If directly discharge, just cause the significant wastage of resource.
This lycopene fungus oil that is rich in multiple carotenoid and enriches aliphatic acid is added in the feed, not only can increase the facilitation of carrotene to animal growth, and can reduce the interpolation cost of grease in the feed, replenish the demand of animal to various aliphatic acid.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of animal feed that utilizes lycopene fungus oil as additive is provided, this animal feed production cost is low, safe and reliable, and business is worth high, helps environmental protection simultaneously.
According to technical scheme provided by the invention: described utilize lycopene fungus oil as the animal feed of additive comprise the lycopene fungus oil of basal feed part and 1.5 (weight) part of 98.5 (weight) part, described lycopene fungus oil come from microbial fermentation produce in the lycopene process refine, the bacterium oil that contains lycopene of generation after the crystalline lycopene.
As a further improvement on the present invention, the content of lycopene in the described lycopene fungus oil is 0.5～1.0%, and unit is a percetage by weight.
As a further improvement on the present invention, described basal feed comprises: sugared source material, 55～60 (weight) part; The protein sources material, 14～16 (weight) part; The cellulose source material, 18～22 (weight) part; Composite mineral matter, 1.4～1.6 (weight) part; Compound amino acid, 0.9～1.1 (weight) part; B B-complex, 0.9～1.1 (weight) part; Adhesive, 1.8～2.2 (weight) part.
As a further improvement on the present invention, described sugared source material comprises: fish meal, 11～13 (weight) part; Dregs of beans, 21～25 parts (weight) part; Peanut meal, 9～11 (weight) part; The dish dregs of rice, 12～14 (weight) part.
As a further improvement on the present invention, described protein sources material adopts corn flour; The cellulose source material adopts wheat bran; Described adhesive adopts the methylol fiber.
As a further improvement on the present invention, described composite mineral matter comprises that the ratio of weight and number of material and each material is: iron, 3.5～4.0 parts; Zinc, 0.45～055 part; Copper, 0.08～0.12 part; Manganese, 0.35～0.4 part; Iodine, 0.014～0.016 part; Selenium, 0.023～0.027 part; Plug with molten metal 0.002～0.003 part; Magnesium, 12～13 parts; Calcium, 4～6 parts; Phosphorus, 6～8 parts; Sodium, 1～3 part; Potassium, 1～3 part; Sulphur, 3～5 parts; 1～5 part of chlorine.
As a further improvement on the present invention, described compound amino acid comprises that the ratio of weight and number of material and each material is: L-lysine, 4～6 parts; The DL-methionine, 18～22 parts; The L-threonine, 2～4 parts; Histidine, 5.5～6.0 parts; Leucine, 12.5～13 parts; Isoleucine, 7.0～7.5 parts; Arginine, 18～20 parts; Phenylalanine, 16.5～17 parts; Tryptophan, 1.5～2.0 parts; Valine, 10～11 parts.
As a further improvement on the present invention, described B B-complex comprises that the ratio of weight and number of material and each material is: V
A, 0.06～0.07 part; V
B1, 0.8～1.2 part; V
B2, 1.8～2.2 parts; V
B6, 0.8～1.2 part; V
C, 35～45 parts; V
D3, 0.045～0.055 part; V
E, 9～11 parts; The DL-calcium pantothenate, 5.5～6.5 parts; Nicotinic acid, 15～17 parts; Folic acid, 0.14～0.16 part; 8～12 parts in ascorbic acid; Pantothenic acid, 35～45 parts; Inositol, 35～45 parts; Vitamin K, 7～9 parts; Vitamin E, 2～4 parts.
As a further improvement on the present invention, the preparation method of described lycopene fungus oil is as follows in detail:
(1) the one-level kind is cultivated: female, the male bacterial strain of trispore Bruce mould that will be deposited in the energy metabolism generation lycopene on the PDA. slant medium inserts respectively in the conical flask that the first order seed culture medium is housed, in temperature is 24～30 ℃, under 160～180 rev/mins the condition, shaken cultivation 44～48 hours obtains the one-level kind;
(2) the secondary kind is cultivated: after one-level kind cultured trispore Bruce mould is female, male bacterial strain is mixed in 1: 1 ratio, kind amount by 1% inserts and is equipped with in the conical flask of secondary kind culture medium, in temperature is 24～30 ℃, under 160～180 rev/mins the condition, shaken cultivation 44～48 hours, obtain the secondary kind, this secondary kind is as the fermentation seed;
(3) fermentation: the fermentation that step (2) is cultivated inserts with seed is equipped with in the stirred fermentor of fermentation medium, is 0.03～0.08MPa at tank pressure, and temperature is 24～30 ℃, and mixing speed is a ventilating fermentation 96～120 hours under 400～600 rev/mins the condition; The cyclisation that when fermenting, adds arsenic pyridine or derivatives thereof class metabolic block agent inhibition lycopene to 48 hours.
(4) extract: collect the wet thallus after step (3) is fermented, wet thallus obtains to be rich in the dry bacterium powder of natural lycopene 70～80 ℃ of following heating, vacuum dryings, adds the extractant extraction again, and the extract decompression concentrates freezing and crystallizing, centrifugal collection coarse crystallization; With this coarse crystallization dissolution filter, freezing and crystallizing once more, centrifugal collection obtains the pure product of lycopene, and filtrate filtered is lycopene fungus oil.
As a further improvement on the present invention, pyridine is adopted in described metabolic block agent; Described extractant adopts vegetable oil or ethyl acetate.
The present invention compared with prior art, advantage is: realized the utilization again of lycopene fungus oil, bioavilability improves, and can not discharge and environment is polluted.Lycopene fungus oil has substituted lycopene feed addictive in the past, reduced feed cost, and the feed safety that makes is reliable, can strengthen the immunologic function of animal, reduces the incidence of disease.
The specific embodiment
The invention will be further described below in conjunction with specific embodiment.
Embodiment 1: the obtaining of lycopene fungus oil:
(1) the one-level kind is cultivated: will be deposited in PDA. slant medium (fresh peeling potato 300g; Glucose 20g; Agar 20g; Running water 1000ml; The pH nature.Behind the packing test tube, 0.1MPa sterilize 20 minutes, (this bacterial strain is called in name female, the male bacterial strain of trispore Bruce mould of the energy metabolism generation lycopene use after chamfered surface water is done): " a kind of trispore Bruce mould fermentation preparation lycopene method of utilizing ", publication number mentions that in the patent documentation of " 1920046 " trispore Bruce mould female bacterium preserving number is: ATCC14272; The male bacterial strain preserving number of trispore Bruce mould is: ATCC14271) insert respectively and the first order seed culture medium is housed (by weight percentage: glucose 1%, starch 3%, corn steep liquor 7%, MgSO
40.02%, surplus is a water, and pH=6.0, loading amount is the 40ml/250ml triangular flask, 0.1MPa sterilization 20 minutes) conical flask in, be 24 ℃ in temperature, under 160 rev/mins the condition, shaken cultivation 48 hours obtains the one-level kind;
(2) the secondary kind is cultivated: after one-level kind cultured trispore Bruce mould is female, male bacterial strain was mixed in 1: 1 ratio, the kind amount by 1% inserted and secondary kind culture medium is housed (by weight percentage: glucose 2%, starch 4%, corn steep liquor 3%, KH
2PO
4, 0.2%, surplus is a water, pH=6.0, loading amount is the 160ml/1000ml triangular flask, 0.1MPa sterilization 20 minutes) conical flask in, be under 24 ℃ the condition, under 160 rev/mins the condition in temperature, shaken cultivation 48 hours obtains the secondary kind, and this secondary kind is as the fermentation seed;
(3) fermentation: the fermentation that step (2) is cultivated is equipped with fermentation medium (by weight percentage: starch 2%, soya-bean cake powder 2%, corn steep liquor 7%, KH with seed by 10% kind amount access
2PO
40.2%, surplus is a water, pH=6.4; Fermentation tank is 30 liters a common stirred fermentor, adorns 20 liters of fermentation mediums, 120 ℃ of sterilizations 40 minutes) stirred fermentor in, be 0.05MPa at tank pressure, temperature is 24 ℃, mixing speed is a ventilating fermentation 120 hours under 400 rev/mins the condition; When fermenting, add the cyclisation that the arsenic pyridine suppresses lycopene to 48 hours.
(4) extract: collect the wet thallus after step (3) is fermented, wet thallus is 70 ℃ of following heating, vacuum dryings, obtain the dry bacterium powder that 900g is rich in natural lycopene, add 10 liters of ethyl acetate extractions again, the extract decompression concentrates, add absolute ethyl alcohol and carry out freezing and crystallizing, 2000 rev/mins of centrifugal collection coarse crystallization 35.64 grams; This coarse crystallization is dissolved with 5 liters of chloroforms, and filter with sand core funnel, add the absolute ethyl alcohol of 2 times of filtrate volumes, freezing and crystallizing 24 hours, 4000 rev/mins of centrifugal collections obtain containing 85% pure product 13.42 grams of lycopene, and filtrate filtered is lycopene fungus oil.
Embodiment 2: feed preparation and crucian are fed:
(1) basal feed preparation: get 12.0 parts of fish meal (available from Lianyungang, Jiangsu Province distillation fish meal factory) by weight, 23.0 parts of dregs of beans (available from the big agricultural byproducts purchase and sale of Yancheng, Jiangsu Province phoenix company), 10.0 parts of peanut meals (available from the big agricultural byproducts purchase and sale of Yancheng, Jiangsu Province phoenix company), 13.0 parts of the dish dregs of rice (available from the big agricultural byproducts purchase and sale of Yancheng, Jiangsu Province phoenix company), 15.0 parts of corn flour (available from the big agricultural byproducts purchase and sale of Yancheng, Jiangsu Province phoenix company), 20.0 parts of wheat brans (available from the big agricultural byproducts purchase and sale of Yancheng, Jiangsu Province phoenix company), 1.5 parts of composite mineral matters, 1.0 parts of compound amino acids, 1.0 parts of B B-complexes, 2.0 parts of methylol fibers; Mix, make basal feed;
(2) feed preparation: get the lycopene fungus oil of three groups of above-mentioned acquisitions, the lycopene concentration of adjusting wherein is respectively 0.50%, 0.75% and 1.00%; Get four groups of the basal feeds of step (1) preparation, in four groups of basal feeds, add 1.5 parts in 1.5 parts and one group cotton seed oil in contrast of lycopene fungus oil that above-mentioned three groups of concentration do not wait respectively, be designated as experimental group 1 (lycopene concentration is 0.50%), experimental group 2 (lycopene concentration is 0.75%), experimental group 3 (lycopene concentration is 1.00%) and control group (not containing lycopene) respectively.Make particle diameter 2.4mm pellet after fully being mixed, with 50～60 ℃ of oven dry, water content is below 10%;
(3) crucian is fed: get the experiment crucian (available from the former seed multiplication farm of Jiangsu Province's Yangzhou aquatic products, a plant is sent out in breed together in Dantu District Bao Yan town, Zhengjiang City, through handling after 5 days, select the crucian that the strong nothing of body is hindered, specification is close, the long 22.4 ± 1.13cm of crucian average body, average weight 180.60 ± 37.23g) 200 tails, be divided into three experimental group and a control group at random, every group 50 tail put in a suitable place to breed respectively in polyethylene net cage (3.0m * 4.0m * 2.5m, specification is 20 orders) and raised.Control group is thrown something and fed and is added the feed of cotton seed oil (not containing lycopene), and experimental group is thrown something and fed respectively and added the feed of variable concentrations lycopene fungus oil, and daily ration, feeding quantity is determined by fish body weight 4.5%, 8: 30 every days, thrown something and fed 2 time at 16: 30.Water temperature is 26～33 ℃ between feeding period, dissolved oxygen 6.5～8.3mg/L, and pH 6.9～7.6, feeding time 50 days.
Embodiment 3: the result detects and analyzes:
Experiment detection reagent and instrument: T-SOD, CAT, MDA measure kit (building up bio-engineering research institute available from Nanjing); Heparin (available from Shanghai bio-engineering corporation); UV-2800 type ultraviolet-uisible spectrophotometer.
(1) growth effect detects: experiment finishes that fish body (n=50) body in the back weighing net cage is long, body weight and liver are heavy, and (experimental data is with mean+SD (Mean ± SD) expression to carry out the statistical analysis of growth rate, rate of body weight gain, survival rate, coefficient of condition and liver body index.SPSS 11.5 softwares are adopted in data statistics, the multiple comparing check experimental group of Duncan differences, P＜0.05 expression significant difference (P＜0.01 expression difference is extremely remarkable).Fish body growth rate, rate of body weight gain, survival rate, coefficient of condition and the reference of liver body index calculation method " inland waters fishery survey of natural resources handbook).
(2) serum T-SOD determination of activity:
Every net cage is randomly drawed 20 tails experiments fish, and the asepsis injector arteria caudalis is got blood, 1% anticoagulant heparin, 4 ℃ are spent the night, 4 ℃ 250r/ minute centrifugal 5 minutes, get upper serum ,-20 ℃ of preservations are standby.
The active detection of T-SOD adopted xanthine oxidase.Get serum to be checked with physiological saline (85%) dilution in 1: 1, sample volume 50 μ L, OD550, detected temperatures＜25 ℃.Reaching 50% o'clock pairing SOD amount with SOD inhibiting rate in every milliliter of reactant liquor is a SOD unit of activity (U), and testing result is as shown in table 1.
(3) change of serum C AT vigor detects:
The CAT vigor detects and adopts ultraviolet spectrometry.Serum sampling amount 100 μ L to be checked, OD405, detected temperatures＜25 ℃.The H that decomposes 1 μ mol during with 25 ℃ of every milliliter of serum each second
2O
2Amount be a unit of activity (U/mL), testing result is as shown in table 1.
(4) Content of MDA detects:
The MDA content detection adopts thiobarbituricacid, and (the tetraethoxypropane concentration of standard solution is 10nmol/L for Thiobarbituric Acid, TBA) colorimetric method.Serum sampling amount 100 μ L to be checked, OD532, detected temperatures＜25 ℃, testing result represents that with nmol/mL testing result is as shown in table 1.
Table 1: lycopene is to the influence of crucian
Interpretation of result:
By table 1 as seen, the experiment crucian in three experimental group and the control group after raising in 50 days, and survival rate all reaches 100%.Experimental group and control group compare, and growth rate and rate of body weight gain all are significantly increased.Experimental group growth rate and rate of body weight gain increase with the raising of lycopene addition, and with 1.00% addition significantly (P＜0.05), there were significant differences (P＜0.05) with 0.75% and 1.00% addition for rate of body weight gain than the show improvement rate for multiple ratio.Along with the raising of experimental group lycopene addition, coefficient of condition is reduction trend, and liver body index is for increasing gradually.When experimental group liver body index is minimum is 3.51%, and coefficient of condition is 1.76%, meets the good growth theory of fish.Therefore, select adding lycopene concentration in the crucian artifical compound feed is that 0.75% lycopene fungus oil will help to improve the growth of culturing crucian.
Lycopene in natural plants nearly all the form with transconfiguration exist, exist with isomeric forms after entering animal body, in the majority with cis-isomer.In animal body, lycopene by little intestinal absorption and with enter lymphatic vessel after chylomicron in the blood plasma combines, be distributed to the histoorgan of whole body with blood circulation, by physics and chemical mode synergy, efficient cancellation singlet oxygen and remove peroxylradicals.Because of molecular structure contains the characteristics of a plurality of conjugated double bonds, make its oxidation resistance in many carotenoid the strongest.Antioxidation and other carrotene relatively, the ability of its cancellation singlet oxygen is 100 times of vitamin E, 2 times of carrotene.
SOD is the antioxidase of removing oxygen radical important in the body, and the increase of its activity can stop and eliminate oxygen radical, and is closely related with the immune level of organism.CAT mainly participates in the active oxygen metabolism process, makes H
2O
2Disproportionation takes place generate H
2O and oxygen molecule, the protection bio-tissue is avoided poisoning, and plays a significant role.MDA is an important MDA in the body, can cause the chain reaction of oxygen radical, and tool amplifies the effect of active oxygen.Therefore SOD has reflected the ability of body removing oxygen radical indirectly, and MDA reflection body is subjected to the degree of oxygen free radical injury.Significantly raise in that SOD is active as a result to rat body Antioxidation Effects, MDA content is reduction trend, illustrates that lycopene has stronger antioxidation.Find all that in different tests lycopene can significantly reduce level of lipid peroxidation, protection biomembrane and DNA avoid the damage of free radical, effective prophylactic generation.Research further confirms, the remarkable negative correlation that is of lycopene levels and a lot of chronic diseases in serum and the tissue.
By table 1 as seen: the T-SOD activity obtains increasing in experimental group and the control group comparison serum, and CAT vigor and MDA content all have certain reduction.The lycopene addition is 0.75% experimental group and control group comparison, T-SOD and CAT significant difference (P＜0.05), and MDA content has reduced 4.55nmol/mL, for minimum in the experimental group.Lycopene has the change of serum C AT of inhibition in the crucian growth and MDA raises, and improves the T-SOD vigor simultaneously.
Claims (10)
1. one kind, it is characterized in that: this feed comprises the basal feed part of 98.5 weight portions and the lycopene fungus oil of 1.5 weight portions, and described lycopene fungus oil comes from microbial fermentation and produce the bacterium oil that contains lycopene that produces in the lycopene process after refinement, crystalline lycopene; The described lycopene fungus oil of described animal feed comes from microbial fermentation and produce the bacterium oil that contains lycopene that produces in the lycopene process after refinement, crystalline lycopene.
2. the animal feed that utilizes lycopene fungus oil as additive as claimed in claim 1 is characterized in that: the content of lycopene in the described lycopene fungus oil is 0.5～1.0%, and unit is a percetage by weight.
3. the animal feed that utilizes lycopene fungus oil as additive as claimed in claim 1 is characterized in that: described basal feed comprises sugared source material, 55～60 weight portions; The protein sources material, 14～16 weight portions; The cellulose source material, 18～22 weight portions; Composite mineral matter, 1.4～1.6 weight portions; Compound amino acid, 0.9～1.1 weight portion; B B-complex, 0.9～1.1 weight portion; Adhesive, 1.8～2.2 weight portions.
4. the animal feed that utilizes lycopene fungus oil as additive as claimed in claim 3 is characterized in that: described sugared source material comprises fish meal, 11～13 weight portions; Dregs of beans, 21～25 parts of weight portions; Peanut meal, 9～11 weight portions; The dish dregs of rice, 12～14 weight portions.
5. the animal feed that utilizes lycopene fungus oil as additive as claimed in claim 3 is characterized in that: described protein sources material adopts corn flour; The cellulose source material adopts wheat bran; Described adhesive adopts the methylol fiber.
6. the animal feed that utilizes lycopene fungus oil as additive as claimed in claim 3 is characterized in that: described composite mineral matter comprises that the ratio of weight and number of material and each material is: iron, 3.5～4.0 parts; Zinc, 0.45～055 part; Copper, 0.08～0.12 part; Manganese, 0.35～0.4 part; Iodine, 0.014～0.016 part; Selenium, 0.023～0.027 part; Plug with molten metal 0.002～0.003 part; Magnesium, 12～13 parts; Calcium, 4～6 parts; Phosphorus, 6～8 parts; Sodium, 1～3 part; Potassium, 1～3 part; Sulphur, 3～5 parts; 1～5 part of chlorine.
7. the animal feed that utilizes lycopene fungus oil as additive as claimed in claim 3 is characterized in that: described compound amino acid comprises that the ratio of weight and number of material and each material is: L-lysine, 4～6 parts; The DL-methionine, 18～22 parts; The L-threonine, 2～4 parts; Histidine, 5.5～6.0 parts; Leucine, 12.5～13 parts; Isoleucine, 7.0～7.5 parts; Arginine, 18～20 parts; Phenylalanine, 16.5～17 parts; Tryptophan, 1.5～2.0 parts; Valine, 10～11 parts.
8. the animal feed that utilizes lycopene fungus oil as additive as claimed in claim 3 is characterized in that: described B B-complex comprises that the ratio of weight and number of material and each material is: V
A, 0.06～0.07 part; V
B1, 0.8～1.2 part; V
B2, 1.8～2.2 parts; V
B6, 0.8～1.2 part; V
C, 35～45 parts; V
D3, 0.045～0.055 part; V
E, 9～11 parts; The DL-calcium pantothenate, 5.5～6.5 parts; Nicotinic acid, 15～17 parts; Folic acid, 0.14～0.16 part; 8～12 parts in ascorbic acid; Pantothenic acid, 35～45 parts; 35～45 parts of inositols; Part; Vitamin K, 7～9 parts; Vitamin E, 2～4 parts.
9. the animal feed that utilizes lycopene fungus oil as additive as claimed in claim 1, it is characterized in that: the preparation method of described lycopene fungus oil is as follows in detail:
(1) the one-level kind is cultivated: female, the male bacterial strain of trispore Bruce mould that will be deposited in the energy metabolism generation lycopene on the PDA. slant medium inserts respectively in the conical flask that the first order seed culture medium is housed, in temperature is 24～30 ℃, under 160～180 rev/mins the condition, shaken cultivation 44～48 hours obtains the one-level kind;
(2) the secondary kind is cultivated: after one-level kind cultured trispore Bruce mould is female, male bacterial strain is mixed in 1: 1 ratio, kind amount by 1% inserts and is equipped with in the conical flask of secondary kind culture medium, in temperature is 24～30 ℃, under 160～180 rev/mins the condition, shaken cultivation 44～48 hours, obtain the secondary kind, this secondary kind is as the fermentation seed;
(3) fermentation: the fermentation that step (2) is cultivated inserts with seed is equipped with in the stirred fermentor of fermentation medium, is 0.03～0.08MPa at tank pressure, and temperature is 24～30 ℃, and mixing speed is a ventilating fermentation 96～120 hours under 400～600 rev/mins the condition; The cyclisation that when fermenting, adds arsenic pyridine or derivatives thereof class metabolic block agent inhibition lycopene to 48 hours.
(4) extract: collect the wet thallus after step (3) is fermented, wet thallus obtains to be rich in the dry bacterium powder of natural lycopene 70～80 ℃ of following heating, vacuum dryings, adds the extractant extraction again, and the extract decompression concentrates freezing and crystallizing, centrifugal collection coarse crystallization; With this coarse crystallization dissolution filter, freezing and crystallizing once more, centrifugal collection obtains the pure product of lycopene, and filtrate filtered is lycopene fungus oil.
10. the animal feed that utilizes lycopene fungus oil as additive as claimed in claim 9 is characterized in that: pyridine is adopted in described metabolic block agent; Described extractant adopts vegetable oil or ethyl acetate.
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| CN103719640A (en) * | 2014-01-28 | 2014-04-16 | 四川农业大学 | Additive capable of improving fish meat quality, using method, application and fish feed |
| CN104397457A (en) * | 2014-12-11 | 2015-03-11 | 四川农业大学 | Nutrition formula for improving gill barrier function, application and feed adopting nutrition formula |
| CN109123236A (en) * | 2017-07-05 | 2019-01-04 | 云南农业大学 | A kind of efficient fish mixed feed with anti-oxidation function |
| CN111543545A (en) * | 2020-05-15 | 2020-08-18 | 内蒙古自治区农牧业科学院 | A feed additive, diet and application for relieving liver damage of dairy cows |
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| 《饲料与畜牧》 20071115 任延利等 《新型饲料添加剂番茄红素的作用与展望》 第19-21页 1-10 , 第11期 2 * |
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| CN102028113B (en) * | 2010-10-18 | 2014-06-04 | 新疆维吾尔自治区畜牧科学院饲料研究所 | Process for preparing daily ration of beef cattle for producing functional beef |
| CN103719640A (en) * | 2014-01-28 | 2014-04-16 | 四川农业大学 | Additive capable of improving fish meat quality, using method, application and fish feed |
| CN104397457A (en) * | 2014-12-11 | 2015-03-11 | 四川农业大学 | Nutrition formula for improving gill barrier function, application and feed adopting nutrition formula |
| CN104397457B (en) * | 2014-12-11 | 2017-07-11 | 四川农业大学 | Improve fish gill barrier function alimentation composition, using and feed |
| CN109123236A (en) * | 2017-07-05 | 2019-01-04 | 云南农业大学 | A kind of efficient fish mixed feed with anti-oxidation function |
| CN109123235A (en) * | 2017-07-05 | 2019-01-04 | 云南农业大学 | A kind of efficient fish mixed feed with anti-oxidation function |
| CN111543545A (en) * | 2020-05-15 | 2020-08-18 | 内蒙古自治区农牧业科学院 | A feed additive, diet and application for relieving liver damage of dairy cows |
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| CN101828641B (en) | 2012-09-05 |
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