CN101756883A - Microbial ecological beauty mask and preparation method thereof - Google Patents
Microbial ecological beauty mask and preparation method thereof Download PDFInfo
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Abstract
本发明公开了一种微生物生态美容面膜的原料配方及制备方法,主要原料为蔗糖、蜂蜜、硫酸铵、磷酸二氢钾、蛋白胨、茶叶、甘草、人参、茯苓、珍珠粉、红茶菌母液,经生物发酵方法制得,本发明制得的微生物生态美容面膜,内含大量的生物活性成分以及益生菌,具有营养、解毒、抑菌、调节皮肤的微生态等功能,是一种性能优良的新型皮肤保健和美容面膜。The invention discloses a raw material formula and a preparation method of a microbial ecological beauty mask. The main raw materials are sucrose, honey, ammonium sulfate, potassium dihydrogen phosphate, peptone, tea, licorice, ginseng, Poria cocos, pearl powder, and red tea fungus mother liquor, which are prepared through a biological fermentation method. The microbial ecological beauty mask prepared by the invention contains a large amount of biologically active ingredients and probiotics, has the functions of nutrition, detoxification, antibacterial, regulating skin microecology, etc., and is a new type of skin health care and beauty mask with excellent performance.
Description
一技术领域a technical field
本发明涉及一种微生物生态美容面膜及其制备方法,属于日用化妆品领域。The invention relates to a microbial ecological beauty mask and a preparation method thereof, belonging to the field of daily cosmetics.
二、背景技术2. Background technology
目前,市场上销售的各种面膜一般都是由护肤营养物质、填充物以及少量的防腐剂组成。虽然这些面膜对皮肤具有吸附有毒物质、提供皮肤营养的功效,但是这些面膜含有的少量防腐剂以及一些有害物质对皮肤都有一定的伤害;同时,为了方便保存和运输,这些面膜的含水量少、质地也不够细腻,这也容易给皮肤带来一定的伤害;再次,这些面膜在美容的时候往往破坏了皮肤的微生态环境,所以容易造成皮肤过敏等症状;同时,对粉刺等微生物引起的皮肤疾病的保健效果一般也建立在面膜成分中对病原菌的短时抑制作用,所以这些美容面膜对一些皮肤的保健、去斑效果欠佳。At present, various facial masks sold on the market are generally composed of skin care nutrients, fillers and a small amount of preservatives. Although these masks have the effect of absorbing toxic substances and providing skin nutrition, the small amount of preservatives and some harmful substances contained in these masks have certain damage to the skin; at the same time, in order to facilitate storage and transportation, these masks contain less water , the texture is not delicate enough, which is easy to cause certain damage to the skin; again, these masks often destroy the micro-ecological environment of the skin during beauty treatment, so it is easy to cause symptoms such as skin allergies; The health care effect of skin diseases is generally also based on the short-term inhibition of pathogenic bacteria in the ingredients of the mask, so these beauty masks have poor health care and spot removal effects on some skins.
三、发明内容3. Contents of the invention
本发明的目的是针对现有技术的不足而提供一种微生物生态美容面膜及其制备方法。细菌纤维素是一些细菌分泌形成的一种良好生物可降解生物材料,它具有成分含量纯,无毒,吸附能力强,弹性大,较高的生物相容性,因此,它是作为面膜的良好基质材料[(马承铸,顾真荣.细菌纤维素生物理化特性和商业用途(综述).上海农业学报2001,17(4):93-98]。The object of the present invention is to provide a kind of microbial eco-beauty facial mask and its preparation method aiming at the deficiencies of the prior art. Bacterial cellulose is a good biodegradable biomaterial secreted by some bacteria. It has pure ingredients, non-toxicity, strong adsorption capacity, high elasticity, and high biocompatibility. Therefore, it is a good choice for facial masks. Matrix materials [(Ma Chengzhu, Gu Zhenrong. Biophysical and chemical properties and commercial applications of bacterial cellulose (review). Shanghai Agricultural Journal 2001, 17(4): 93-98].
细菌纤维素在医学上的应用研究表明:细菌纤维素具有良好的生物适应性,很好的韧性强度和水合度,有利于皮肤组织生长和限制感染。其次,细菌纤维素膜可作为缓释药物的载体携带各种药物,利于皮肤表面给药,促使创面的愈合和康复。这种材料的优点在于可有效缓解疼痛,有良好的附着性,可有效防止细菌的入侵感染,促进伤口的快速愈合,对于水分及电解物有良好的通透性。此外,与传统的材料相比,这种材料更有利于健康皮肤的生长[J.D.Fontana,A.M.De Souza,C.K.Fontana,et al.Acetobacter cellulose pellicleas a temporary skin substitute[J].Applied Biochemisty and Biotechnology.1990,24/25:253-264;贾士儒,欧斌宇,傅强.新型生物材料一细菌纤维素[J].食品与发酵工业,2001,27(1):54-58]。Research on the application of bacterial cellulose in medicine shows that: bacterial cellulose has good biological adaptability, good toughness, strength and hydration, which is beneficial to the growth of skin tissue and limits infection. Secondly, the bacterial cellulose membrane can be used as a slow-release drug carrier to carry various drugs, which is beneficial to drug delivery on the skin surface and promotes wound healing and rehabilitation. The advantage of this material is that it can effectively relieve pain, has good adhesion, can effectively prevent bacterial invasion and infection, promote rapid wound healing, and has good permeability to water and electrolytes. In addition, compared with traditional materials, this material is more conducive to the growth of healthy skin [J.D.Fontana, A.M.De Souza, C.K.Fontana, et al.Acetobacter cellulose pellicles a temporary skin substitute[J].Applied Biochemistry and Biotechnology.1990 , 24/25: 253-264; Jia Shiru, Ou Binyu, Fu Qiang. New biological material-bacterial cellulose [J]. Food and Fermentation Industry, 2001, 27(1): 54-58].
目前,醋酸菌是主要生产细菌纤维素的菌种。传统发酵工艺中,由醋酸菌纯种培养或醋酸菌和其他微生物混合培养,可产生含有丰富纤维素的发酵食品,如纳塔和红茶菌等。红茶菌是以茶叶浸出液通过发酵制成的一种保健饮料,红茶菌液中含有大量的醋酸菌和酵母菌以及少量乳酸菌。在生产红茶菌的过程中,在培养液的表面形成一层胶状生物膜,其主要成分是细菌纤维素和活性益生菌如醋酸杆菌、酵母菌等,它是由醋酸菌分泌、结晶形成的细菌纤维素包裹红茶菌菌体形成的一种菌膜。红茶菌是一种对人体具有重要保健作用的保健饮料,对红茶菌液的成分分析表明:除了含有大量的活性益生菌之外,它还含有大量对人体有益的活性成分。法国的Blanc(1991)从红茶菌溶液中分离出乙醇、乳酸、乙酸、葡萄糖酸葡萄糖醛酸以及抗菌物质-地衣酸[Blanc,P.J.1996.Characterization of the teafungus metabolites.Biotechnol.Lett.18:139-142.]。Liu等(1995)用液相色谱分析自中国台湾三个城市的红茶菌样品中,从中分析出甘油、乙酸、乙醇,并指出酵母和醋酸菌产生的乙醇、乙酸可防止其他微生物的侵染(Liu,C.H.,W.H.Hsu,F.L.Lee & C.C.Liao.1996.The isolation andidentification of microbes from a fermented tea beverage,Haipao,and their interactions during Haipao fermentation.FoodMicrobiol.13:407-415]。此外,还有红茶菌菌液含有抗菌蛋白,对许多病原菌有抑制作用[谢俊杰,钟青萍,佘世望,晏学明.红茶菌液抑菌作用的研究.食品科技.2000,6:59-60]。At present, acetic acid bacteria are the main strains that produce bacterial cellulose. In the traditional fermentation process, the pure culture of acetic acid bacteria or the mixed culture of acetic acid bacteria and other microorganisms can produce fermented foods rich in cellulose, such as nata and kombucha. Kombucha is a health drink made by fermenting tea extract. Kombucha liquid contains a large amount of acetic acid bacteria, yeast and a small amount of lactic acid bacteria. In the process of producing kombucha, a colloidal biofilm is formed on the surface of the culture medium, and its main components are bacterial cellulose and active probiotics such as acetic acid bacteria, yeast, etc., which are secreted and crystallized by acetic acid bacteria Bacterial cellulose is a bacterial film formed by wrapping the kombucha fungus. Kombucha is a health drink that has an important health care effect on the human body. The composition analysis of the kombucha liquid shows that in addition to containing a large amount of active probiotics, it also contains a large number of active ingredients that are beneficial to the human body. Blanc (1991) of France separated ethanol, lactic acid, acetic acid, glucuronic acid glucuronic acid and antibacterial substance-lichenic acid from the kombucha solution [Blanc, P.J.1996.Characterization of the teafungus metabolites.Biotechnol.Lett.18: 139- 142.]. Liu et al. (1995) analyzed glycerol, acetic acid and ethanol from the kombucha samples of three cities in Taiwan, China by liquid chromatography, and pointed out that ethanol and acetic acid produced by yeast and acetic acid bacteria can prevent the infection of other microorganisms ( Liu, C.H., W.H.Hsu, F.L.Lee & C.C.Liao.1996.The isolation and identification of microbes from a fermented tea beverage, Haipao, and their interactions during Haipao fermentation.FoodMicrobiol.13:407-415]. Bacterial liquid contains antibacterial protein, which has inhibitory effect on many pathogenic bacteria [Xie Junjie, Zhong Qingping, She Shiwang, Yan Xueming. Research on antibacterial effect of black tea bacterial liquid. Food Science and Technology. 2000, 6: 59-60].
Petrovska等(2000)用薄层色谱法(thin layer chromatography,TLC)和紫外吸收光谱法从红茶菌中鉴定出四种可溶性维生素,它们分别是VB1、VB6、VB12和VC[Petrovska B,Petrushevska-Tozi B,Lidija.2000.Mineral and water soluble vitamin content in theKombucha drink.International.Journal of Food Science & Technology,35(2),201-205]。Petrovska et al. (2000) identified four soluble vitamins from kombucha fungi by thin layer chromatography (TLC) and ultraviolet absorption spectroscopy, which are VB 1 , VB 6 , VB 12 and VC [Petrovska B, Petrushevska-Tozi B, Lidija. 2000. Mineral and water soluble vitamin content in the Kombucha drink. International. Journal of Food Science & Technology, 35(2), 201-205].
研究表明:上述活性成分如醋酸菌、酵母菌、乳酸菌、蛋白质、葡萄糖酸、葡萄糖醛酸、乙醇、醋酸、乳酸、维生素、抑菌蛋白等具有营养、解毒、抑制病原菌生长、调节人体微生态的功能,因此,红茶菌液和菌膜的这些功能对于保养人体外表的皮肤保健也具有重要的应用价值。Studies have shown that the above active ingredients such as acetic acid bacteria, yeast, lactic acid bacteria, protein, gluconic acid, glucuronic acid, ethanol, acetic acid, lactic acid, vitamins, antibacterial proteins, etc. Therefore, these functions of kombucha liquid and bacterial film also have important application value for the skin care of maintaining the appearance of the human body.
本发明者发现,通过设计特殊的加有中药的红茶菌培养基,利用红茶菌发酵产生大量的活性物质以及形成红茶菌菌膜,这种红茶菌膜是一种优良的微生物生态美容面膜;利用红茶菌发酵形成的细菌纤维素菌膜作载体,它既可以吸附红茶菌液中大量的生物活性物质,又可以直接用作美容的面膜;它既可以吸附皮肤上的有毒物质,又可以调节皮肤的微生态环境,因此,它是一种具有重要价值和应用前景的化妆品。The inventors found that by designing a special kombucha culture medium added with traditional Chinese medicine, using kombucha fermentation to produce a large amount of active substances and forming a kombucha fungus film, this kombucha fungus film is an excellent microbial ecological beauty mask; The bacterial cellulose film formed by the fermentation of kombucha is used as a carrier. It can not only absorb a large amount of biologically active substances in the kombucha liquid, but also can be directly used as a facial mask; it can not only absorb toxic substances on the skin, but also regulate skin. Therefore, it is a cosmetic with important value and application prospect.
本发明的目的由以下技术措施实现,其中所述原料份数除了特殊说明外,均为重量份数。The purpose of the present invention is achieved by the following technical measures, wherein the parts of raw materials are parts by weight unless otherwise specified.
这种微生物生态美容面膜的起始原料配方组份为:蔗糖5--10份;蜂蜜5--10份;硫酸铵0.2-1.0份;磷酸二氢钾0.2-1.0份;蛋白胨0.5-1.0份;、茶叶5----10份;甘草5----10份;人参5----10份;茯苓5---10份;珍珠粉0.2---1.0份;红茶菌母液5---20份;水600-900份。The starting material formula components of this microbial ecological beauty mask are: 5-10 parts of sucrose; 5-10 parts of honey; 0.2-1.0 parts of ammonium sulfate; 0.2-1.0 parts of potassium dihydrogen phosphate; 0.5-1.0 parts of peptone ;, 5----10 parts of tea; 5----10 parts of licorice; 5----10 parts of ginseng; ---20 parts; water 600-900 parts.
其中的红茶菌母液包含大量的活性醋酸菌、酵母菌以及乳酸菌。The kombucha mother liquor contains a large amount of active acetic acid bacteria, yeast and lactic acid bacteria.
这种微生物生态美容面膜的制备方法:The preparation method of this microbial ecology beauty mask:
(1)按重量配比称原料;(1) weighing raw materials by weight ratio;
(2)将茶叶、甘草、茯苓按中药煎煮1小时,除掉渣后得药液I;(2) Tealeaves, Radix Glycyrrhizae, Poria cocos are decocted by traditional Chinese medicine 1 hour, get medicinal liquid I after getting rid of dregs;
(3)将蔗糖、蜂蜜、硫酸铵、磷酸二氢钾、蛋白胨、珍珠粉和水一起加热溶解,得药液II;(3) Heat and dissolve sucrose, honey, ammonium sulfate, potassium dihydrogen phosphate, peptone, pearl powder and water together to obtain liquid medicine II;
(4)将两种药液(药液I和药液II)混合、冷却到30℃以下时接种红茶菌母液;(4) Mix two medicinal liquids (medicinal liquid I and medicinal liquid II) and inoculate the kombucha mother liquor when cooling to below 30°C;
(5)将配制好的溶液倒入无菌容器,用透气纱布封口后在暗处培养,发酵培养15--30天后,培养液上层形成的菌膜就是微生物生态美容面膜的成品。(5) Pour the prepared solution into a sterile container, seal it with air-permeable gauze and cultivate it in the dark. After 15-30 days of fermentation and cultivation, the bacterial film formed on the upper layer of the culture solution is the finished product of the microbial ecological beauty mask.
本发明具有如下优点:The present invention has the following advantages:
1.此面膜是醋酸菌分泌的纤维素包裹益生菌形成的生物膜,是一种真正的纯天然生物面膜,不含一般面膜保存所需要的防腐剂如乙醇、丙三醇等,因此,它具有无毒、刺激性小的优点;1. This mask is a biofilm formed by cellulose secreted by acetic acid bacteria and wrapped with probiotics. It is a real pure natural biological mask, and it does not contain preservatives such as ethanol and glycerin that are required for the preservation of general masks. Therefore, it It has the advantages of non-toxic and less irritating;
2.此面膜的含水量高、质地非常温柔,因此,它具有很好的保湿和深层洁肤的功能;2. This mask has high water content and very gentle texture, so it has good moisturizing and deep cleansing functions;
3.此面膜含有的醋酸等酸性物质可以软化角质层,有助于消除皱纹、消除雀斑等;3. Acidic substances such as acetic acid contained in this mask can soften the cuticle, which helps to eliminate wrinkles and freckles;
4.此面膜中含有的抑菌物质如醋酸、抑菌蛋白可以在短期内快速抑制人体皮肤中的病原菌的生长;因为醋酸菌是一种好氧的益生菌,它可以在人体皮肤表面进行一定量的繁殖,因此,它可以很好抑制病原菌的生长,调节人体皮肤表面的微生态环境,对于由于病原菌引起的皮肤疾病如面部粉刺、痤疮具有很有的治疗和保健效果。4. The antibacterial substances contained in this mask, such as acetic acid and antibacterial protein, can quickly inhibit the growth of pathogenic bacteria in human skin in a short period of time; because acetic acid bacteria are a kind of aerobic probiotics, it can carry out a certain amount of bacteria on the surface of human skin. Therefore, it can well inhibit the growth of pathogenic bacteria, regulate the micro-ecological environment on the surface of human skin, and has a great therapeutic and health care effect on skin diseases caused by pathogenic bacteria, such as facial acne and acne.
5.可快速用于人体面部或其他部位的美容,由于此面膜是一种含水量很高的面膜,使用方便;同时,由于营养物质容易为人体皮肤吸收,所以可以达到快速的美容效果;5. It can be quickly used for beautifying the face or other parts of the human body. Because this mask is a mask with a high water content, it is easy to use; at the same time, because the nutrients are easily absorbed by the human skin, it can achieve a rapid beautifying effect;
6.由于本面膜可以轻松的更换,所以可以先用一块面膜进行皮肤洁净;然后用另一块面膜进行皮肤保养,这样避免了有害物质对皮肤的再次毒害,这样可以取得理想的保养效果;6. Since this mask can be easily replaced, one piece of mask can be used to clean the skin first; then another piece of mask can be used to maintain the skin, so as to avoid the poisoning of the skin by harmful substances again, so as to achieve the ideal maintenance effect;
四具体实施方式Four specific implementation methods
实施例1:取400克水,分别加入5克茶叶、5克甘草、5克茯苓中药煎煮1小时,除掉渣后得药液I;再取400克水,将50克蔗糖、1克蜂蜜、1克硫酸铵、1克磷酸二氢钾、1克蛋白胨、1克珍珠粉和水一起加热溶解,得药液II;将两种药液(药液I和药液II)混合、冷却到30℃以下时接种红茶菌母液200克;将配制好的溶液倒入圆形的敞口无菌玻璃瓶,用透气纱布封口后在暗处培养,发酵培养15-30天后,在瓶中培养液的表层就会形成圆性的生物膜,这就是微生物生态美容面膜的成品,培养液可以用来保养面膜,也是皮肤保养的营养液。Embodiment 1: get 400 grams of water, add respectively 5 grams of tea leaves, 5 grams of Radix Glycyrrhizae, 5 grams of Poria cocos Chinese medicine and decoct for 1 hour, get medicinal liquid I after removing the residue; Get 400 grams of water again, mix 50 grams of sucrose, 1 gram of Heat and dissolve honey, 1 gram of ammonium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of peptone, 1 gram of pearl powder, and water to obtain liquid medicine II; mix the two liquid medicines (liquid I and liquid II), and cool Inoculate 200 grams of kombucha mother liquor when the temperature is below 30°C; pour the prepared solution into a round open sterile glass bottle, seal it with breathable gauze, and cultivate it in a dark place. After 15-30 days of fermentation, culture it in the bottle A circular biofilm will be formed on the surface of the liquid, which is the finished product of the microbial ecological beauty mask. The culture liquid can be used to maintain the mask, and it is also a nutrient solution for skin maintenance.
实施例2:取400克水,分别加入1克茶叶、1克甘草、1克茯苓中药煎煮1小时,除掉渣后得药液I;再取400克水,将1克蔗糖、1克蜂蜜、1克硫酸铵、1克磷酸二氢钾、1克蛋白胨、1克珍珠粉和水一起加热溶解,得药液II;将两种药液(药液I和药液II)混合、冷却到30℃以下时接种红茶菌母液200克;将配制好的溶液倒入圆形的敞口无菌玻璃瓶,用透气纱布封口后在暗处培养,发酵培养15-30天后,在瓶中培养液的表层就会形成圆性的生物膜,这就是微生物生态美容面膜的成品,培养液可以用来保养面膜,也是保养皮肤的营养液。Embodiment 2: Get 400 grams of water, add 1 gram of tealeaves, 1 gram of Radix Glycyrrhizae, 1 gram of Poria cocos Chinese medicine decocting 1 hour respectively, get medicinal liquid I after getting rid of slag; Get 400 grams of water again, mix 1 gram of sucrose, 1 gram of Heat and dissolve honey, 1 gram of ammonium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of peptone, 1 gram of pearl powder, and water to obtain liquid medicine II; mix the two liquid medicines (liquid I and liquid II), and cool Inoculate 200 grams of kombucha mother liquor when the temperature is below 30°C; pour the prepared solution into a round open sterile glass bottle, seal it with breathable gauze, and cultivate it in a dark place. After 15-30 days of fermentation, culture it in the bottle A circular biofilm will be formed on the surface of the liquid, which is the finished product of the microbial ecological beauty mask. The culture liquid can be used to maintain the mask, and it is also a nutrient solution for maintaining the skin.
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