CN101543523B - Uyghur drug compound recipe extracting technology for chiccory - Google Patents
Uyghur drug compound recipe extracting technology for chiccory Download PDFInfo
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Abstract
Description
技术领域technical field
本发明涉及一种维吾尔药“护肝布祖热”的提取新工艺，由该工艺获得的提取物可作为保肝护肝类药物的用途，还可作为治疗肝寒、胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。The present invention relates to a new extraction process of Uighur medicine "Liver-protecting Buzure". The extract obtained by the process can be used as liver-protecting and liver-protecting drugs, and can also be used to treat liver cold, stomach pain, spleen resistance and rib pain , Arthralgia, rheumatism, urinary system diseases.
背景技术Background technique
随着生活水平的提高以及社会经济、生活模式的变化，人类的健康理念和追求生活质量的意识逐步增强，当今人类疾病谱与医学治疗模式发生了巨大的改变。由于化学合成药物表现出开发难度大、费用高昂、研制周期长、毒副作用明显的缺陷，国际医药市场对治疗慢性病、免疫性疾病、特高发疾病以及养生保健、延年益寿等方面具有独特疗效的天然植物药产品需求急剧上升，有资料表明：世界上大约有60％的人口几乎完全依赖于植物药来治疗疾病。新疆是世界四大长寿区之一，千百年来神秘的维医维药一直维护着新疆各族人民的身心健康。在颇具特色的维医理论指导下，维医维药建立了以实践经验为主体，以整体治疗为特征的科学体系。随着现代社会的发展和人类生活节奏的加快，越来越多的疾病危害着人类的健康，疾病的发生涉及多种因素、多靶点通路和网络调控，故西医治疗学中所采用的针对单一靶点的传统治疗方案，难以奏效。而维医药从整体入手的治疗方案，既是单味药，也通过多途径，多靶点，作用于多个证候的组合，产生预防及治疗效果，多年的实践表明其具有良好的临床疗效，表现出了极大的优势和特色。With the improvement of living standards and changes in social economy and life patterns, human health concepts and awareness of quality of life have gradually increased, and today's human disease spectrum and medical treatment models have undergone tremendous changes. Due to the defects of difficult development, high cost, long development cycle, and obvious toxic and side effects of chemically synthesized drugs, the international pharmaceutical market has natural plants with unique curative effects in the treatment of chronic diseases, immune diseases, extremely high-incidence diseases, health care, and longevity. The demand for pharmaceutical products has risen sharply. According to data, about 60% of the world's population relies almost entirely on plant medicines to treat diseases. Xinjiang is one of the four longevity areas in the world. For thousands of years, the mysterious Uighur medicine has been maintaining the physical and mental health of people of all ethnic groups in Xinjiang. Under the guidance of distinctive Uyghur medicine theory, Uyghur medicine and medicine have established a scientific system with practical experience as the main body and holistic treatment as the characteristic. With the development of modern society and the acceleration of the pace of human life, more and more diseases are endangering human health. The occurrence of diseases involves various factors, multi-target pathways and network regulation. Traditional treatment options with a single target are difficult to work. Uighur medicine starts with the overall treatment plan. It is not only a single medicine, but also acts on the combination of multiple syndromes through multiple channels and multiple targets to produce preventive and therapeutic effects. Years of practice have shown that it has good clinical curative effect. Showed great advantages and features.
部颁标准收载的“护肝布祖热”颗粒是维族人民千百年来用于治疗各种肝炎的最常用的组方药物之一，其主要成分为芹菜子、芹菜根、菊苣子、菟丝子、菊苣根、茴香根皮、小茴香。根据临床的实验，护肝布组热颗粒治疗病毒性肝炎、药物性肝炎和酒精性肝炎引起的肝硬化，肝脏代谢中毒性损伤以及脂肪肝等疾病引起的乏力、腹胀、肝区不适、黄疸、腹水、脂肪肝的改善，效果迅速明显，是治疗肝炎安全有效的药物。复方中的主要药材毛菊苣在国内外具有广泛的研究基础，大量的国内外文献报道说明，毛菊苣提取物能够明显降低由于四氯化碳引起的大鼠肝脏SGPT，SGOT，Alkp and T.Bil水平，具有最好抗肝毒活性，对由于四氯化碳引起的大鼠肝损伤具有很好的保护作用，对酒精肝和肝硬化都有良好的作用。研究人员对菊苣中提取的有效成分进行分离后得到了七叶内酯，它能够保护由于扑热息痛和四氯化碳引起的肝损伤，这在一定程度上能够解释菊苣作为治疗肝损伤药的原因。维吾尔医药理论中，在保肝、护肝的处方中应用毛菊苣等维吾尔药材十分普遍。The "Huganbuzure" granule recorded in the ministerial standards is one of the most commonly used prescription drugs used by the Uyghur people for thousands of years to treat various hepatitis. Its main ingredients are celery seed, celery root, chicory seed, Cuscuta, chicory root, fennel root bark, cumin. According to clinical experiments, Huganbu Group Hot Granules can treat liver cirrhosis caused by viral hepatitis, drug-induced hepatitis and alcoholic hepatitis, fatigue, abdominal distension, liver discomfort, jaundice, The improvement of ascites and fatty liver is quick and obvious, and it is a safe and effective drug for treating hepatitis. The main medicinal material in the compound, Chicory, has extensive research at home and abroad. A large number of domestic and foreign literature reports show that the extract of Chicory can significantly reduce SGPT, SGOT, Alkp and T.Bil in rat liver caused by carbon tetrachloride. Level, has the best anti-hepatotoxic activity, has a good protective effect on rat liver damage caused by carbon tetrachloride, and has a good effect on alcoholic liver and liver cirrhosis. The researchers obtained escinolide after separating the active ingredients extracted from chicory, which can protect the liver damage caused by paracetamol and carbon tetrachloride, which can explain the reason why chicory is used as a drug for liver damage to a certain extent. In Uygur medical theory, it is very common to use Uygur medicinal materials such as chicory in liver protection and liver protection prescriptions.
原标准中“护肝布祖热”采用传统的水煎煮法提取，提取工艺简单，有效成分不明确，无质量控制标准。因此，本发明通过对“护肝布祖热”方中各味药材的化学研究后，将方中药材分类后采用不同溶剂提取，最大程度提取方中有效成分，并对提取物进行了治疗肝损伤的药效学研究。研究采用灌胃方法，其结果表明维吾尔药“护肝布祖热”采用本发明提供的提取工艺得到的提取物具有改善四氯化碳致急性肝损伤模型小鼠ALT及AST的作用，可作为保肝护肝类药物的用途，还可作为治疗肝寒、胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。In the original standard, "Huganbuzure" was extracted by the traditional water decoction method, the extraction process was simple, the active ingredients were not clear, and there was no quality control standard. Therefore, the present invention, after chemical research on the medicinal materials in the "Huganbuzure" prescription, classifies the medicinal materials in the prescription and extracts them with different solvents to extract the active ingredients in the prescription to the greatest extent, and treats the extracts to treat liver disease. Pharmacodynamic studies of injury. The research adopts the method of intragastric administration, and the results show that the extract obtained from the Uighur medicine "Hugan Buzure" using the extraction process provided by the present invention has the effect of improving the ALT and AST of carbon tetrachloride-induced acute liver injury model mice, and can be used as The use of liver-protecting drugs can also be used as a drug for treating liver cold, stomach pain, spleen obstruction and rib pain, joint and bone pain, rheumatism, and urinary system diseases.
发明内容Contents of the invention
本发明目的在于提供一种对现有的维吾尔药复方“护肝布祖热”采用新的提取的工艺，将处方芹菜子、小茴香采用水提取，得到挥发油和水提取物，将菊苣子、菟丝子、芹菜根、茴香根皮、菊苣根采用醇提取，得到醇提取物，将水提取物和醇提取物混合均匀后喷洒 入挥发油即可得到“护肝布祖热”提取物，并将获得的提取物按常规制药方法制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂；或将提取物与中、西药配伍制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂。经药效试验表明：维吾尔药“护肝布祖热”采用本发明提供的提取工艺得到的提取物具有改善四氯化碳致急性肝损伤模型小鼠ALT及AST的作用，可作为保肝护肝类药物的用途，还可作为治疗肝寒、胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。The purpose of the present invention is to provide a new extraction process for the existing Uighur medicine compound "Hugan Buzure", extract the prescription celery seeds and cumin with water to obtain volatile oil and water extracts, and extract the chicory seeds, Cuscuta, celery root, fennel root bark, and chicory root are extracted with alcohol to obtain alcohol extract, and the water extract and alcohol extract are mixed evenly and then sprayed into volatile oil to obtain the "Liver-protecting Buzure" extract, which will be obtained The extract is made into capsules or tablets or oral liquid or granules or sustained-release preparations or controlled-release preparations according to conventional pharmaceutical methods; or the extract is made into capsules or tablets or oral liquid or granules or sustained-release formulations or controlled release formulations. The drug efficacy test shows that the Uighur medicine "Liver-protecting Buzure" obtained by using the extraction process provided by the present invention has the effect of improving ALT and AST of carbon tetrachloride-induced acute liver injury model mice, and can be used as a protective agent for liver protection. The use of liver drugs can also be used as a drug for treating liver cold, stomach pain, spleen obstruction rib pain, joint bone pain, rheumatism, and urinary system diseases.
本发明所述的一种维吾尔药复方“护肝布祖热”提取工艺，按下列步骤进行：The extraction process of a Uighur medicine compound "Huganbuzure" according to the present invention is carried out according to the following steps:
a、取芹菜子、小茴香，加入5-15倍量的水，浸泡1-6小时，按常规方法提取挥发油，提取时间1-8小时，收取挥发油，备用；a. Take celery seeds and fennel, add 5-15 times the amount of water, soak for 1-6 hours, extract the volatile oil according to the conventional method, the extraction time is 1-8 hours, collect the volatile oil, and set aside;
b、将步骤a中剩余的水溶液过滤，浓缩，干燥后粉碎成粉末，得到水提物，备用；b. Filter the remaining aqueous solution in step a, concentrate, dry and pulverize into powder to obtain the water extract for subsequent use;
c、取菊苣子、菟丝子、芹菜根、茴香根皮和菊苣根混合后，进行粉碎，加入5-15倍的醇，回流提取1-5次，每次2小时，过滤，合并滤液，减压浓缩至无醇味，干燥后粉碎成粉末，得到醇提物，备用；c. After mixing chicory seeds, dodder seeds, celery root, fennel root bark and chicory root, grind them, add 5-15 times of alcohol, reflux extraction 1-5 times, each time for 2 hours, filter, combine the filtrate, and reduce pressure Concentrate to no alcohol smell, dry and pulverize into powder to obtain alcohol extract, set aside;
d、将步骤b的水提物与步骤c的醇提物充分混合均匀，然后喷洒步骤a收取的挥发油，即可得到维吾尔药“护肝布祖热”的提取物；d. Fully mix the water extract of step b with the ethanol extract of step c, and then spray the volatile oil collected in step a to obtain the extract of the Uighur medicine "Liver-protecting Buzure";
e、将步骤d的提取物按常规制药方法制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂；或将提取物与中、西药配伍制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂。e. The extract of step d is made into capsules or tablets or oral liquid or granule or sustained-release preparation or controlled-release preparation according to conventional pharmaceutical methods; or the extract is made into capsules or tablets or oral liquid by combining Chinese and Western medicines Or granules or sustained-release preparations or controlled-release preparations.
步骤c中醇为甲醇或乙醇或异丙醇或丁醇，优选乙醇，其浓度为50-95％。The alcohol in step c is methanol or ethanol or isopropanol or butanol, preferably ethanol, and its concentration is 50-95%.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗保肝护肝类药物的用途。The "liver-protecting Buzure" extract obtained by the extraction process is used for the preparation of liver-protecting and liver-protecting drugs.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗胃痛、 脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。The "Hugan Buzure" extract obtained by the extraction process is used as a medicine for the treatment of stomach pain, spleen block pain, joint bone pain, rheumatism, and urinary system diseases.
通过本发明所述的提取工艺获得的提取物，具有改善急性肝损伤小鼠ALT及AST的作用，可作为保肝护肝类药物的用途，还可作为治疗肝寒、胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。同时该工艺中采取秦皮乙素为指标成分，增加了制剂的稳定性，便于制剂质量控制。The extract obtained by the extraction process of the present invention has the effect of improving ALT and AST in mice with acute liver injury, can be used as liver-protecting and liver-protecting drugs, and can also be used as a treatment for liver cold, stomach pain, spleen resistance and rib pain , Arthralgia, rheumatism, urinary system diseases. Simultaneously, in the process, acetaminophen is used as an index component, which increases the stability of the preparation and facilitates the quality control of the preparation.
具体实施方式Detailed ways
实施例1Example 1
a、取芹菜子53g、小茴香53g加入5倍量的水，浸泡1小时，按常规方法提取挥发油，提取时间1小时，收取挥发油0.3ml，备用；a. Take 53g of celery seeds and 53g of fennel, add 5 times the amount of water, soak for 1 hour, extract the volatile oil according to the conventional method, the extraction time is 1 hour, collect 0.3ml of volatile oil, and set aside;
b、将步骤a中剩余的水溶液过滤，浓缩，干燥后粉碎成粉末，得到水提物，备用；b. Filter the remaining aqueous solution in step a, concentrate, dry and pulverize into powder to obtain the water extract for subsequent use;
c、取菊苣子16g、菟丝子4g、芹菜根16g、茴香根皮16g、菊苣根8g混合后，进行粉碎，加入5倍的浓度为50％乙醇，回流提取1次，时间2小时，过滤，合并滤液，减压浓缩至无醇味，干燥后粉碎成粉末，得到醇提物13g，备用；c. Take 16g of chicory seeds, 4g of dodder seeds, 16g of celery root, 16g of fennel root bark, and 8g of chicory root, mix them, grind them, add 5 times the concentration of 50% ethanol, reflux extraction once for 2 hours, filter, and combine The filtrate was concentrated under reduced pressure until it had no alcohol smell, dried and pulverized into powder to obtain 13 g of alcohol extract, which was set aside;
d、将步骤b的水提物与步骤c的醇提物充分混合均匀，然后喷洒步骤a收取的挥发油，即可得到维吾尔药“护肝布祖热”的提取物；d. Fully mix the water extract of step b with the ethanol extract of step c, and then spray the volatile oil collected in step a to obtain the extract of the Uighur medicine "Liver-protecting Buzure";
e、将步骤d的提取物按常规制药方法制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂；或将提取物与中、西药配伍制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂。e. The extract of step d is made into capsules or tablets or oral liquid or granule or sustained-release preparation or controlled-release preparation according to conventional pharmaceutical methods; or the extract is made into capsules or tablets or oral liquid by combining Chinese and Western medicines Or granules or sustained-release preparations or controlled-release preparations.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗保肝护肝类药物的用途。The "liver-protecting Buzure" extract obtained by the extraction process is used for the preparation of liver-protecting and liver-protecting drugs.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。The "liver-protecting Buzure" extract obtained by the extraction process is used as a medicine for treating stomachache, spleen obstruction and rib pain, joint and bone pain, rheumatism and urinary system diseases.
实施例2Example 2
a、取芹菜子53g、小茴香53g加入8倍量的水，浸泡2小时，按常 规方法提取挥发油，提取时间6小时，收取挥发油0.9ml，备用；a. Take 53g of celery seeds and 53g of fennel, add 8 times the amount of water, soak for 2 hours, extract the volatile oil according to the conventional method, the extraction time is 6 hours, collect 0.9ml of volatile oil, and set aside;
b、将步骤a中剩余的水溶液过滤，浓缩，干燥后粉碎成粉末，得到水提物，备用；b. Filter the remaining aqueous solution in step a, concentrate, dry and pulverize into powder to obtain the water extract for subsequent use;
c、取菊苣子16g、菟丝子4g、芹菜根16g、茴香根皮16g、菊苣根8g混合后，进行粉碎，加入8倍量浓度为60％甲醇，回流提取2次，每次2小时，过滤，合并滤液，减压浓缩至无醇味，干燥后粉碎成粉末，得到醇提物16g，备用；c, get chicory seed 16g, dodder seed 4g, celery root 16g, fennel root bark 16g, chicory root 8g after mixing, pulverize, add 8 times of concentration and be 60% methanol, reflux extraction 2 times, each 2 hours, filter, The filtrates were combined, concentrated under reduced pressure until there was no alcohol smell, dried and pulverized into powder to obtain 16 g of alcohol extract, which was set aside;
d、将步骤b的水提物与步骤c的醇提物充分混合均匀，然后喷洒步骤a收取的挥发油，即可得到维吾尔药“护肝布祖热”的提取物；d. Fully mix the water extract of step b with the ethanol extract of step c, and then spray the volatile oil collected in step a to obtain the extract of the Uighur medicine "Liver-protecting Buzure";
e、将步骤d的提取物按常规制药方法制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂；或将提取物与中、西药配伍制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂。e. The extract of step d is made into capsules or tablets or oral liquid or granule or sustained-release preparation or controlled-release preparation according to conventional pharmaceutical methods; or the extract is made into capsules or tablets or oral liquid by combining Chinese and Western medicines Or granules or sustained-release preparations or controlled-release preparations.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗保肝护肝类药物的用途。The "liver-protecting Buzure" extract obtained by the extraction process is used for the preparation of liver-protecting and liver-protecting drugs.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。The "liver-protecting Buzure" extract obtained by the extraction process is used as a medicine for treating stomachache, spleen obstruction and rib pain, joint and bone pain, rheumatism and urinary system diseases.
实施例3Example 3
a、取芹菜子53g、小茴香53g加入10倍量的水，浸泡4小时，按常规方法提取挥发油，提取时间5小时，收取挥发油0.6ml，备用；a. Take 53g of celery seeds and 53g of fennel, add 10 times the amount of water, soak for 4 hours, extract the volatile oil according to the conventional method, the extraction time is 5 hours, collect 0.6ml of volatile oil, and set aside;
b、将步骤a中剩余的水溶液过滤，浓缩，干燥后粉碎成粉末，得到水提物，备用；b. Filter the remaining aqueous solution in step a, concentrate, dry and pulverize into powder to obtain the water extract for subsequent use;
c、取菊苣子16g、菟丝子4g、芹菜根16g、茴香根皮16g、菊苣根8g混合后，进行粉碎，加入10倍量浓度为70％异丙醇，回流提取4次，每次2小时，过滤，合并滤液，减压浓缩至无醇味，干燥后粉碎成粉末，得到醇提物13g，备用；c, get chicory seed 16g, dodder seed 4g, celery root 16g, fennel root bark 16g, chicory root 8g after mixing, pulverize, add 10 times of concentration and be 70% isopropanol, reflux extraction 4 times, each 2 hours, Filtrate, combine the filtrates, concentrate under reduced pressure until there is no alcohol smell, dry and pulverize into powder to obtain 13g of alcohol extract, set aside;
d、将步骤b的水提物与步骤c的醇提物充分混合均匀，然后喷洒步骤a收取的挥发油，即可得到维吾尔药“护肝布祖热”的提取物；d. Fully mix the water extract of step b with the ethanol extract of step c, and then spray the volatile oil collected in step a to obtain the extract of the Uighur medicine "Liver-protecting Buzure";
e、将步骤d的提取物按常规制药方法制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂；或将提取物与中、西药配伍制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂。e. The extract of step d is made into capsules or tablets or oral liquid or granule or sustained-release preparation or controlled-release preparation according to conventional pharmaceutical methods; or the extract is made into capsules or tablets or oral liquid by combining Chinese and Western medicines Or granules or sustained-release preparations or controlled-release preparations.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗保肝护肝类药物的用途。The "liver-protecting Buzure" extract obtained by the extraction process is used for the preparation of liver-protecting and liver-protecting drugs.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。The "liver-protecting Buzure" extract obtained by the extraction process is used as a medicine for treating stomachache, spleen obstruction and rib pain, joint and bone pain, rheumatism and urinary system diseases.
实施例4Example 4
a、取芹菜子53g、小茴香53g加入15倍量的水，浸泡6小时，按常规方法提取挥发油，提取时间8小时，收取挥发油1.1ml，备用；a. Take 53g of celery seeds and 53g of fennel, add 15 times the amount of water, soak for 6 hours, extract the volatile oil according to the conventional method, the extraction time is 8 hours, collect 1.1ml of volatile oil, and set aside;
b、将步骤a中剩余的水溶液过滤，浓缩，干燥后粉碎成粉末，得到水提物，备用；b. Filter the remaining aqueous solution in step a, concentrate, dry and pulverize into powder to obtain the water extract for subsequent use;
c、取菊苣子16g、菟丝子4g、芹菜根16g、茴香根皮16g、菊苣根8g混合后，进行粉碎，加入15倍浓度为95％乙醇，回流提取5次，每次2小时，过滤，合并滤液，减压浓缩至无醇味，干燥后粉碎成粉末，得到醇提物7.6g，备用；c. Take 16 g of chicory seeds, 4 g of dodder seeds, 16 g of celery root, 16 g of fennel root bark, and 8 g of chicory root, mix them, grind them, add 15 times the concentration of 95% ethanol, and reflux extraction 5 times, each time for 2 hours, filter and combine The filtrate was concentrated under reduced pressure until it had no alcohol smell, dried and pulverized into powder to obtain 7.6 g of alcohol extract, which was set aside;
d、将步骤b的水提物与步骤c的醇提物充分混合均匀，然后喷洒步骤a收取的挥发油，即可得到维吾尔药“护肝布祖热”的提取物；d. Fully mix the water extract of step b with the ethanol extract of step c, and then spray the volatile oil collected in step a to obtain the extract of the Uighur medicine "Liver-protecting Buzure";
e、将步骤d的提取物按常规制药方法制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂；或将提取物与中、西药配伍制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂。e. The extract of step d is made into capsules or tablets or oral liquid or granule or sustained-release preparation or controlled-release preparation according to conventional pharmaceutical methods; or the extract is made into capsules or tablets or oral liquid by combining Chinese and Western medicines Or granules or sustained-release preparations or controlled-release preparations.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗保肝护肝类药物的用途。The "liver-protecting Buzure" extract obtained by the extraction process is used for the preparation of liver-protecting and liver-protecting drugs.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。The "liver-protecting Buzure" extract obtained by the extraction process is used as a medicine for treating stomachache, spleen obstruction and rib pain, joint and bone pain, rheumatism and urinary system diseases.
实施例5Example 5
a、取芹菜子53g、小茴香53g加入25倍量的水，浸泡4小时，按常 规方法提取挥发油，提取时间4小时，收取挥发油0.8ml，备用；a. Take 53g of celery seeds and 53g of fennel, add 25 times the amount of water, soak for 4 hours, extract the volatile oil according to the conventional method, the extraction time is 4 hours, collect 0.8ml of volatile oil, and set aside;
b、将步骤a中剩余的水溶液过滤，浓缩，干燥后粉碎成粉末，得到水提物，备用；b. Filter the remaining aqueous solution in step a, concentrate, dry and pulverize into powder to obtain the water extract for subsequent use;
c、取菊苣子16g、菟丝子4g、芹菜根16g、茴香根皮16g、菊苣根8g混合后，进行粉碎，加入12倍浓度为85％丁醇，回流提取2次，每次2小时，过滤，合并滤液，减压浓缩至无醇味，干燥后粉碎成粉末，得到醇提物5.2g，备用；c, get chicory seed 16g, dodder seed 4g, celery root 16g, fennel root bark 16g, chicory root 8g after mixing, pulverize, add 12 times concentration and be 85% butanol, reflux extraction 2 times, each 2 hours, filter, The filtrates were combined, concentrated under reduced pressure until there was no alcohol smell, dried and pulverized into powder to obtain 5.2 g of alcohol extract, which was set aside;
d、将步骤b的水提物与步骤c的醇提物充分混合均匀，然后喷洒步骤a收取的挥发油，即可得到维吾尔药“护肝布祖热”的提取物；d. Fully mix the water extract of step b with the ethanol extract of step c, and then spray the volatile oil collected in step a to obtain the extract of the Uighur medicine "Liver-protecting Buzure";
e、将步骤d的提取物按常规制药方法制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂；或将提取物与中、西药配伍制成胶囊或片剂或口服液或冲剂或缓释制剂或控释制剂。e. The extract of step d is made into capsules or tablets or oral liquid or granule or sustained-release preparation or controlled-release preparation according to conventional pharmaceutical methods; or the extract is made into capsules or tablets or oral liquid by combining Chinese and Western medicines Or granules or sustained-release preparations or controlled-release preparations.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗保肝护肝类药物的用途。The "liver-protecting Buzure" extract obtained by the extraction process is used for the preparation of liver-protecting and liver-protecting drugs.
所述的提取工艺获得的“护肝布祖热”提取物作为制备治疗胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。The "liver-protecting Buzure" extract obtained by the extraction process is used as a medicine for treating stomachache, spleen obstruction and rib pain, joint and bone pain, rheumatism and urinary system diseases.
实施例6Example 6
本发明对维吾尔药“护肝布祖热”采用新的提取工艺获得的提取物，能改善CCl4引起的小鼠急性肝损伤作用。结果见表：The extract obtained by adopting a new extraction process to the Uighur medicine "Liver-protecting Buzure" can improve the effect of acute liver injury in mice caused by CCl 4 . The results are shown in the table:
结果表明：采用本发明提供的新工艺获得的提取物，可作为制备治疗保肝护肝类药物的用途，或作为制备治疗肝寒、胃痛、脾阻肋痛、关节骨痛、风湿病、泌尿系统疾病的药物用途。The results show that: the extract obtained by the new process provided by the present invention can be used as the preparation and treatment of liver-protecting and liver-protecting drugs, or as a preparation for the treatment of liver cold, stomach pain, spleen resistance rib pain, arthralgia, rheumatism, urinary Drug use in systemic diseases.
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