CN101464332A - Selenium diagnosis/measuring reagent kit and method for measuring selenium concentration - Google Patents
Selenium diagnosis/measuring reagent kit and method for measuring selenium concentration Download PDFInfo
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- CN101464332A CN101464332A CNA2007101921259A CN200710192125A CN101464332A CN 101464332 A CN101464332 A CN 101464332A CN A2007101921259 A CNA2007101921259 A CN A2007101921259A CN 200710192125 A CN200710192125 A CN 200710192125A CN 101464332 A CN101464332 A CN 101464332A
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- glutathione
- galactose
- selenium
- coenzyme
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 68
- 239000011669 selenium Substances 0.000 title claims abstract description 47
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 45
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000003745 diagnosis Methods 0.000 title claims description 11
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 46
- 239000005515 coenzyme Substances 0.000 claims abstract description 27
- 239000003381 stabilizer Substances 0.000 claims abstract description 25
- 108010024636 Glutathione Proteins 0.000 claims abstract description 22
- 229960003180 glutathione Drugs 0.000 claims abstract description 22
- 108010015133 Galactose oxidase Proteins 0.000 claims abstract description 21
- VYPPEYAOCURAAE-GUCUJZIJSA-N D-galacto-hexodialdose Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C=O VYPPEYAOCURAAE-GUCUJZIJSA-N 0.000 claims abstract description 20
- 108010014369 galactose dehydrogenase Proteins 0.000 claims abstract description 19
- 238000002835 absorbance Methods 0.000 claims abstract description 13
- 238000005259 measurement Methods 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 3
- 102000006587 Glutathione peroxidase Human genes 0.000 claims description 22
- 108700016172 Glutathione peroxidases Proteins 0.000 claims description 22
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 19
- 239000005864 Sulphur Substances 0.000 claims description 19
- 238000006243 chemical reaction Methods 0.000 claims description 16
- 238000013016 damping Methods 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 10
- 229930182830 galactose Natural products 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 230000001419 dependent effect Effects 0.000 claims description 3
- WNXJCQJNRYHLIO-GEMLJDPKSA-N (2s)-2-amino-5-[[(2r)-1-(carboxymethylamino)-1-oxo-3-sulfanylpropan-2-yl]amino]-5-oxopentanoic acid;hydrate Chemical compound O.OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O WNXJCQJNRYHLIO-GEMLJDPKSA-N 0.000 claims description 2
- PHOQVHQSTUBQQK-MGCNEYSASA-N D-galactono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@H]1O PHOQVHQSTUBQQK-MGCNEYSASA-N 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 5
- RWSXRVCMGQZWBV-PHDIDXHHSA-N L-Glutathione Natural products OC(=O)[C@H](N)CCC(=O)N[C@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-PHDIDXHHSA-N 0.000 claims 2
- 229940093476 ethylene glycol Drugs 0.000 claims 2
- 235000011187 glycerol Nutrition 0.000 claims 2
- 101710122677 L-galactose dehydrogenase Proteins 0.000 claims 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 1
- 235000011130 ammonium sulphate Nutrition 0.000 claims 1
- 239000004615 ingredient Substances 0.000 claims 1
- 239000003755 preservative agent Substances 0.000 claims 1
- 239000000376 reactant Substances 0.000 abstract description 4
- 238000004737 colorimetric analysis Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 238000002965 ELISA Methods 0.000 abstract 1
- DTQFKZRVARUELC-GEMLJDPKSA-N [S].OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O Chemical compound [S].OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O DTQFKZRVARUELC-GEMLJDPKSA-N 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 238000007689 inspection Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 9
- NQMRYBIKMRVZLB-UHFFFAOYSA-N methylamine hydrochloride Chemical compound [Cl-].[NH3+]C NQMRYBIKMRVZLB-UHFFFAOYSA-N 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- 230000000630 rising effect Effects 0.000 description 3
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- 238000001391 atomic fluorescence spectroscopy Methods 0.000 description 2
- 238000002354 inductively-coupled plasma atomic emission spectroscopy Methods 0.000 description 2
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- CXONXVMMINSQBV-NNYOXOHSSA-N (2r,3r,4s,5r)-5-[[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxymethyl]-2-(3-carbamothioylpyridin-1-ium-1-yl)-4-hydroxyoxolan-3-olate Chemical compound NC(=S)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)[O-])=C1 CXONXVMMINSQBV-NNYOXOHSSA-N 0.000 description 1
- 108010072481 Galactose Dehydrogenases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- XJLXINKUBYWONI-NNYOXOHSSA-O NADP(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-O 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 229940065287 selenium compound Drugs 0.000 description 1
- 150000003343 selenium compounds Chemical class 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a kit for diagnosing/measuring selenium by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay of selenium dependency. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of selenium, and belongs to the technical field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, coenzyme, glutathione sulfur, D-GALACTO-HEXODIALDOSE, glutathione peroxydase, galactose oxidase, galactose dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree/velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of selenium.
Description
Technical field
The present invention relates to a kind of selenium diagnosis/determination kit, the invention still further relates to the method for measuring selenium concentration simultaneously, belong to medical science/food/environmental test determination techniques field.
Background technology
Selenium is biological essential trace element, and plant selenium is the main source that the human and animal obtains selenium, and the too much absorption selenium of human body can cause selenosis.The selenium element can make the peroxide breakdown in the human body, protection cell membrane, the heart, kidney, lung, eye etc.; Can remove the free radical of human body, anti-ageing, strengthen immune function of human body, can suppress multiple carcinogen, human body lacks selenium can bring out angiocardiopathy and cancer.
In conjunction with both at home and abroad about the various detection methods of selenium. Chang Yong assay method wherein: as atomic absorption spectrography (AAS) (AAS), atomic fluorescence spectrometry (AFS) and ICP-AES (ICP-AES) etc.; Atomic absorption spectrum (AAS) has become the main method of trace element analysis as element special efficacy detecting device and GC coupling.Also have in addition, use liquid chromatography tandem mass spectrometry (LC-MS-MS) and identify selenium compound.
Summary of the invention
The technical problem to be solved in the present invention is: propose a kind of dependent enzymic colorimetric of selenium (Enzymatic Colorimetric Method) and enzyme (even) united method (Couple Reaction) technology utilized, metering/continuous monitoring reduced form nicotinamide coenzyme (reduced coenzyme) is in the variation of 340nm wavelength place absorbance, measured the method for selenium concentration, simultaneously, the present invention also will provide in order to realize the selenium diagnosis/determination kit of this method, adopt this reagent not only can be ultraviolet analyser or half, carry out measuring selenium concentration on the automatic clinical chemistry analyzer, and finding speed is fast, the accuracy height, thereby can obtain practical applying.
Method for measuring selenium concentration principle of the present invention is as follows:
Glutathione two sulphur+2 water
Glutathione peroxidase/Se
2 glutathione+hydrogen peroxide
Hydrogen peroxide+D-galacto-hexodialdose
Galactose oxidaseGalactose
+ oxygen
Galactose+coenzyme
Galactose dehydrogenaseThe D-galactonolactone+
Reduced coenzyme
This method is utilized the dependent glutathione peroxidase of selenium (glutathione peroxidase; EC1.11.1.9) enzyme (idol) connection galactose oxidase (galactose oxidase; EC1.1.3.9), galactose dehydrogenase (galactose dehydrogenases; EC1.1.1.120; EC1.1.1.48) enzyme ' s reaction speeding colourimetry/end-point method.Glutathione peroxidase enzymolysis glutathione two reaction of Salmon-Saxl produce hydrogen peroxide, the effect of uniting galactose oxidase, galactose dehydrogenase again by (idol), coenzyme (not having absorption peak at the 340nm place) reduces the most at last becomes reduced coenzyme (absorption peak being arranged at the 340nm place), thereby measured degree/speed that reduced coenzyme rises in 340nm place absorbance, by measuring degree/speed that 340nm place absorbance rises, can calculate the concentration of selenium.
Experiment shows, takes all factors into consideration from the accuracy of measurement result and economy two aspects of preparation cost, no matter is single agent, two agent or three doses, and the selenium diagnosis/determination kit of the present invention of following composition relation is comparatively desirable:
Damping fluid 100mmol/L
Stabilizing agent 500mmol/L
Coenzyme 3mmol/L
Glutathione peroxidase 10000U/L
Galactose oxidase 12000U/L
Galactose dehydrogenase 12000U/L
Glutathione two sulphur 10mmol/L
D-GALACTO-HEXODIALDOSE 8mmol/L
Selenium diagnosis/determination kit of the present invention can be single agent, comprising:
Damping fluid, stabilizing agent, coenzyme, glutathione peroxidase, galactose oxidase, galactose dehydrogenase, glutathione two sulphur, D-GALACTO-HEXODIALDOSE.
Kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
Also above-mentioned single agent reagent can be made into following pair of agent reagent:
Reagent 1
Damping fluid, stabilizing agent, coenzyme, glutathione two sulphur, D-GALACTO-HEXODIALDOSE.
Reagent 2
Damping fluid, stabilizing agent, glutathione peroxidase, galactose oxidase, galactose dehydrogenase.
Coenzyme, glutathione peroxidase, galactose oxidase, galactose dehydrogenase, glutathione two sulphur, the position of D-GALACTO-HEXODIALDOSE in reagent 1 or reagent 2 can not limit.Kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
Above-mentioned single agent reagent can also be made into following three doses of reagent:
Reagent 1
Damping fluid, stabilizing agent, coenzyme, glutathione two sulphur, D-GALACTO-HEXODIALDOSE.
Reagent 2
Damping fluid, stabilizing agent, galactose oxidase, galactose dehydrogenase.
Reagent 3
Damping fluid, stabilizing agent, glutathione peroxidase.
Coenzyme, glutathione peroxidase, galactose oxidase, galactose dehydrogenase, glutathione two sulphur, the position of D-GALACTO-HEXODIALDOSE in reagent 1, reagent 2 or reagent 3 can not limit.Kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
No matter be single agent, two agent or three doses, the present invention measures the method for selenium concentration, and its coenzyme can be NADP
+, NAD
+Or thio-NAD
+In a kind of.
Embodiment
The present invention is further illustrated below in conjunction with examples of implementation.
Embodiment one
The selenium diagnosing/determining reagent of present embodiment is single reagent, comprising:
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Coenzyme 3mmol/L
Glutathione peroxidase 10000U/L
Galactose oxidase 12000U/L
Galactose dehydrogenase 12000U/L
Glutathione two sulphur 10mmol/L
D-GALACTO-HEXODIALDOSE 8mmol/L
Reagent divides the bottle of packing into after all dissolving and preparing, and carries out freeze drying, makes powdered reagent; Before the use, add pure water, use after redissolving.
On automatic clinical chemistry analyzer, set: 37 ℃ of temperature, 10 minutes reaction time, initial absorbance≤0.1, test predominant wavelength 340nm, test commplementary wave length 405nm, the volume ratio of tested selenium sample and reagent is 1/25, the Direction of Reaction is positive reaction (reaction of rising), about about 1 minute of time delay is about 5 minutes detection times.
After adding sample and reagent, make them mixed and have reaction, reactant places under the Biochemical Analyzer the most at last, detects degree/speed that predominant wavelength 340nm absorbance rises, thereby calculates the concentration of selenium.
Embodiment two
The selenium diagnosing/determining reagent of present embodiment is double reagent, comprising:
Reagent 1
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 50mmol/L
Coenzyme 3mmol/L
Glutathione two sulphur 10mmol/L
D-GALACTO-HEXODIALDOSE 8mmol/L
Reagent 2
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Glutathione peroxidase 10000U/L
Galactose oxidase 12000U/L
Galactose dehydrogenase 12000U/L
Reagent divides the bottle of packing into after all dissolving and preparing, and makes liquid double reagent, can directly use.
On automatic clinical chemistry analyzer, set: 37 ℃ of temperature, 10 minutes reaction time, initial absorbance≤0.1, test predominant wavelength 340nm, test commplementary wave length 405nm, the volume ratio of tested selenium sample and reagent 1, reagent 2 is 2/20/5, the Direction of Reaction is positive reaction (reaction of rising), about about 1 minute of time delay is about 5 minutes detection times.
After adding sample and reagent, make them mixed and have reaction, reactant places under the Biochemical Analyzer the most at last, detects degree/speed that predominant wavelength 340nm absorbance rises, thereby calculates the concentration of selenium.
Embodiment three
The selenium diagnosing/determining reagent of present embodiment is three reagent, comprising:
Reagent 1
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 50mmol/L
Coenzyme 3mmol/L
Glutathione two sulphur 10mmol/L
D-GALACTO-HEXODIALDOSE 8mmol/L
Reagent 2
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Galactose oxidase 12000U/L
Galactose dehydrogenase 12000U/L
Reagent 3
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Glutathione peroxidase 10000U/L
Reagent divides the bottle of packing into after all dissolving and preparing, and makes liquid three reagent, can directly use.
When measuring selenium concentration, on automatic clinical chemistry analyzer, set: 37 ℃ of temperature, 10 minutes reaction time, initial absorbance≤0.1, test predominant wavelength 340nm, test commplementary wave length 405nm, the volume ratio of tested selenium sample and reagent 1, reagent 2, reagent 3 is 4/40/5/5, the Direction of Reaction is positive reaction (reaction of rising), and about about 1 minute of time delay is about 5 minutes detection times.
After adding sample and reagent, make them mixed and have reaction, reactant places under the Biochemical Analyzer the most at last, detects degree/speed that predominant wavelength 340nm absorbance rises, thereby calculates the concentration of selenium.
The applicant adopts the assay method of putting down in writing in the above summary of the invention all can reach purpose of the present invention through experimental verification, in view of situation such as determination step and above embodiment roughly the same, do not separately enumerate.
In a word, experimental results show that: adopt assay method of the present invention can draw required measurement result by general biochemical analyzer fully---the blank reagent absorbance changes (Δ A/min)≤0.0010; Absorbance time response curve should linearly rise; Reagent can be surveyed effectively, and (R 〉=0.99) linear range can reach 0.5mmol/L; The inaccuracy of reagent test, its relative deviation be no more than ± and 7%; The coefficient of variation (CV)≤2% of the precision of reagent test (repeatability); The sensitivity of reagent can reach that 0.064 ± 0.032 Δ A/mmol/L---the present invention is highly sensitive, degree of accuracy good, and the linear range broadness is enough to easy to utilize.
Claims (6)
1. method for measurement of concentration that utilizes the selenium of dependent enzymic colorimetric of selenium and enzyme-linked method, its method principle is as follows:
Glutathione two sulphur+2 water
Glutathione peroxidase/Se
2 glutathione+hydrogen peroxide
Hydrogen peroxide+D-galacto-hexodialdose
Galactose oxidaseGalactose
+ oxygen
Galactose+coenzyme
Galactose dehydrogenaseThe D-galactonolactone+
Reduced coenzyme
The end reaction thing is placed under ultraviolet analyser or half, the automatic clinical chemistry analyzer, detect degree/speed that predominant wavelength 340nm absorbance rises, calculate the concentration measurement result of selenium.
2. selenium diagnosis/determination kit, principal ingredient comprises:
Damping fluid 20---500mmol/L
Stabilizing agent 1---4000mmol/L
DPN diphosphopyridine nucleotide---6mmol/L
Glutathione peroxidase 1000---80000U/L
Galactose oxidase 1000---80000U/L
Galactose dehydrogenase 1000---80000U/L
Glutathione two sulphur 1---50mmol/L
D-GALACTO-HEXODIALDOSE 1——50mmol/L
The concentration of reagent composition not necessarily is only limited to above-mentioned scope; Effect is better in this scope, and outside this scope, reagent still can reagentia.
It is characterized in that: kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
3. according to the described selenium diagnosis/determination kit of claim 2, it is characterized in that:
Form single agent reagent by damping fluid, stabilizing agent, coenzyme, glutathione peroxidase, galactose oxidase, galactose dehydrogenase, glutathione two sulphur, D-GALACTO-HEXODIALDOSE.
4. according to the described selenium diagnosis/determination kit of claim 2, it is characterized in that:
Form two agent reagent by damping fluid, stabilizing agent, coenzyme, glutathione peroxidase, galactose oxidase, galactose dehydrogenase, glutathione two sulphur, D-GALACTO-HEXODIALDOSE; Reagent 1 is made up of damping fluid, stabilizing agent, coenzyme, glutathione two sulphur, D-GALACTO-HEXODIALDOSE; Reagent 2 is made up of damping fluid, stabilizing agent, glutathione peroxidase, galactose oxidase, galactose dehydrogenase.Coenzyme, glutathione peroxidase, galactose oxidase, galactose dehydrogenase, glutathione two sulphur, the position of D-GALACTO-HEXODIALDOSE in reagent 1 or reagent 2 can not limit.
5. according to the described selenium diagnosis/determination kit of claim 2, it is characterized in that:
Form multi-agent reagent by damping fluid, stabilizing agent, coenzyme, glutathione peroxidase, galactose oxidase, galactose dehydrogenase, glutathione two sulphur, D-GALACTO-HEXODIALDOSE; Reagent 1 is made up of damping fluid, stabilizing agent, coenzyme, glutathione two sulphur, D-GALACTO-HEXODIALDOSE; Reagent 2 is made up of damping fluid, stabilizing agent, galactose oxidase, galactose dehydrogenase; Reagent 3 is made up of damping fluid, stabilizing agent, glutathione peroxidase.Coenzyme, glutathione peroxidase, galactose oxidase, galactose dehydrogenase, glutathione two sulphur, the position of D-GALACTO-HEXODIALDOSE in reagent 1, reagent 2 or reagent 3 can not limit.
6. according to the described selenium diagnosis/determination kit of claim 2, it is characterized in that: also comprise stabilizing agent 1-4000mmol/L or 0.1%-100% volume ratio.Described stabilizing agent is: ammonium sulfate (AmmoniaSulfate), glycerine (Glycerol), propylene glycol (Propylene Glycol), ethylene glycol (Ethyleneglycol) and at least one of the preservatives.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101921259A CN101464332A (en) | 2007-12-19 | 2007-12-19 | Selenium diagnosis/measuring reagent kit and method for measuring selenium concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101921259A CN101464332A (en) | 2007-12-19 | 2007-12-19 | Selenium diagnosis/measuring reagent kit and method for measuring selenium concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101464332A true CN101464332A (en) | 2009-06-24 |
Family
ID=40805008
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101921259A Pending CN101464332A (en) | 2007-12-19 | 2007-12-19 | Selenium diagnosis/measuring reagent kit and method for measuring selenium concentration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101464332A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590105A (en) * | 2011-12-26 | 2012-07-18 | 广州市食品工业研究所有限公司 | Chromazurine beam splitting photometry determining content of aluminum by removing interference of negative ions |
| CN102590109A (en) * | 2012-01-13 | 2012-07-18 | 南京农业大学 | Method for extracting and determining activity of glutathione peroxidase in tea trees |
| CN111198185A (en) * | 2020-02-20 | 2020-05-26 | 迈科若(苏州)医疗科技有限公司 | Enzyme catalysis detection method of selenium monosaccharide |
-
2007
- 2007-12-19 CN CNA2007101921259A patent/CN101464332A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590105A (en) * | 2011-12-26 | 2012-07-18 | 广州市食品工业研究所有限公司 | Chromazurine beam splitting photometry determining content of aluminum by removing interference of negative ions |
| CN102590105B (en) * | 2011-12-26 | 2014-03-26 | 广州市食品工业研究所有限公司 | Chromazurine beam splitting photometry determining content of aluminum by removing interference of negative ions |
| CN102590109A (en) * | 2012-01-13 | 2012-07-18 | 南京农业大学 | Method for extracting and determining activity of glutathione peroxidase in tea trees |
| CN111198185A (en) * | 2020-02-20 | 2020-05-26 | 迈科若(苏州)医疗科技有限公司 | Enzyme catalysis detection method of selenium monosaccharide |
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