CN101422168B - New method for controlling rot knot nematode disease of protected vegetable - Google Patents
New method for controlling rot knot nematode disease of protected vegetable Download PDFInfo
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- CN101422168B CN101422168B CN2008101882672A CN200810188267A CN101422168B CN 101422168 B CN101422168 B CN 101422168B CN 2008101882672 A CN2008101882672 A CN 2008101882672A CN 200810188267 A CN200810188267 A CN 200810188267A CN 101422168 B CN101422168 B CN 101422168B
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- 238000000034 method Methods 0.000 title claims abstract description 22
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 28
- 241000244206 Nematoda Species 0.000 title abstract description 20
- 241000243785 Meloidogyne javanica Species 0.000 claims abstract description 35
- 239000002689 soil Substances 0.000 claims abstract description 16
- 229920002101 Chitin Polymers 0.000 claims abstract description 15
- 241001465752 Purpureocillium lilacinum Species 0.000 claims abstract description 13
- 239000003337 fertilizer Substances 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000008187 granular material Substances 0.000 claims description 3
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- 239000000126 substance Substances 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 4
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- 230000009977 dual effect Effects 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 23
- XOKSLPVRUOBDEW-UHFFFAOYSA-N pinane Chemical compound CC1CCC2C(C)(C)C1C2 XOKSLPVRUOBDEW-UHFFFAOYSA-N 0.000 description 20
- 241000219109 Citrullus Species 0.000 description 19
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 19
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- 240000003768 Solanum lycopersicum Species 0.000 description 12
- 238000010790 dilution Methods 0.000 description 10
- 239000012895 dilution Substances 0.000 description 10
- 229930006728 pinane Natural products 0.000 description 10
- 238000003971 tillage Methods 0.000 description 9
- 244000241257 Cucumis melo Species 0.000 description 5
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 5
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- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
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- 231100000053 low toxicity Toxicity 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000243784 Meloidogyne arenaria Species 0.000 description 2
- 241000243787 Meloidogyne hapla Species 0.000 description 2
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- 238000003825 pressing Methods 0.000 description 2
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Abstract
本发明公开了一种防治蔬菜根结线虫病的新方法。其特征是在蔬菜定植前1-2周用甲壳素、阿罗蒎茲、淡紫拟青霉和杀线先锋中的一种或两种来处理苗床，定植前用福气多1.0Kg-2.0Kg/亩与满地乐20Kg-60Kg/亩混合处理土壤，定植后再配合用生物制剂，具有使用方便，安全有效，既能有效控制根结线虫病，又能促进蔬菜植株生长，具有药肥双重效果，并能够减轻农药大剂量使用所带来的环境污染，前期通过化学杀线虫剂福气多压低土壤中的线虫数量，通过甲壳素和药肥满地乐增加了蔬菜的抗逆性，定植后期通过生物制剂甲壳素、阿罗蒎茲、淡紫拟青霉和杀线先锋中的任一种灌根来调控土壤中残留的线虫数量，达到了生物-化学协同防控蔬菜根结线虫病害的作用，具有很高的推广价值。The invention discloses a new method for preventing and treating vegetable root-knot nematode disease. It is characterized in that the seedbed is treated with one or two of chitin, aropine, Paecilomyces lilacinus and line-killing pioneer 1-2 weeks before vegetable planting, and Fufuduo 1.0Kg-2.0Kg is used before planting Mix 20Kg-60Kg/mu with Mandile to treat the soil, and then use biological agents after planting. It is easy to use, safe and effective. It can not only effectively control root-knot nematode disease, but also promote the growth of vegetable plants. It has dual functions of medicine and fertilizer. effect, and can reduce the environmental pollution caused by the use of large doses of pesticides. In the early stage, the chemical nematicide Fufuduo suppressed the number of nematodes in the soil, and increased the stress resistance of vegetables through chitin and medicine Mandile. In the later stage of planting The number of nematodes remaining in the soil is regulated by any one of the biological agents chitin, arropine, Paecilomyces lilacinus, and nematocide, achieving the role of biological-chemical synergistic control of vegetable root-knot nematode diseases , has high promotional value.
Description
Technical field:
The present invention relates to a kind of new method of facility for prevention and control root knot nematode disease evil.
Background technology:
The many in the world countries and regions of plant nematode take place and cause harm, and kind is varied.In the world; The generation of plant nematode and harm are along with the variation of the development of agriculture and forestry and tillage and cultivation system is serious day by day; Add up according to Esser; Till the nineteen ninety whole world reported find plant nematode 207 belong to 4832 kinds (Liu Weizhi, a section imperial jade seal .2000. plant pathogeny line insect is learned. Beijing: Chinese agriculture publishing house, p1-2).According to estimates, it is 12.3% that plant nematode is caused the year percent of loss of world staple crops, above 1,000 hundred million dollars; And actual loss is considerably beyond estimation; Reason be the harm that causes of many nematodes because of do not have visible symptom in field or specialization characteristic do not arouse attention (Wang Shouhua. the fruit tree nematology. Beijing: Chinese agriculture science and technology publishing house, 1994.P.1-5), in addition; The new discovery of in the world wide nematode being caused harm is also in continuous increase; And the agricultural cultivation system of some renewals makes the nematode problem more outstanding, moreover nematode and other biological interaction and direct or indirect influence that plant is produced are also in rapid increase.The harm that nematode is caused is more hidden than other biology in this sense! At present the harmful nematode of plant there is kind more than 3000 approximately, mainly contains root-knot nematode, SCN, pine wood nematode, sweet potato stem nematode etc. in China.Root-knot nematode is the one type of maximum nematode of causing harm on the agricultural; After disease takes place, the general underproduction about 10%, serious in more than 75%; Even total crop failure (A.G.Whitehead, 1998.Plant Nematode Control.ISBN0851991882 CABInternational).The root-knot nematode that works the mischief in China mainly comprises 4 kinds, i.e. Meloidogyne incognita (Meloidogyneincognita), peanut root-knot nematode (M.arenaria), northern root-knot nematode (M.hapla) and javanese root knot nematode (M.javarnica).Root-knot nematode is infected root system of plant with the form of larva, the ensuing crop underproduction significantly of being injured, even total crop failure, and the generation of isochrone parasitosis has aggravated the harm of soil-borne diseases such as fusarium wilt, verticillium wilt, damping off again.
Control to root-knot nematode at present lacks practicality and high efficiency and continuable improvement technology, and chemical nematode killing agent can only be controlled nematode population in a short time, still has cost height, potentially contaminated, holds problems such as imitating weak point, pesticide resistance.Reasonably cultivating system can play certain preventive and therapeutic effect; But crop and resistant variety that can crop rotation in protection ground be limited; Therefore, the harm of root-knot nematode has become the outstanding problem of restriction pollution-free vegetable production sustainable development and the guarantee of capital food security.Efficient, the practical and continuable root-knot nematode Prevention Technique system of research is to produce at present to go up the key technology of being badly in need of.
Summary of the invention:
The facility for prevention and control root knot nematode disease evil new method that the purpose of this invention is to provide a kind of efficient, low toxicity, low-residual.
For realizing above-mentioned purpose; The present invention adopts following technical scheme: a kind of new method of facility for prevention and control root knot nematode disease evil; This method has overcome the single chemical method of traditional usefulness and has prevented and treated the shortcoming of root-knot nematode and a kind of chemical agent (happy lot is many) and bio-fertilizer (chitin, completely happy) and biopesticide (sieve's Ah pinane is microemulsion, Paecilomyces lilacinus, the line pioneer extremely now) low toxicity that combines, efficient, the safe method of preventing and treating are provided; Concrete grammar is mainly caught seedling stage, setting date and vegetative period three controls periods, carry out grow seedlings, 4 key links such as whole ground, field planting, field management.Evenly water with 500 times of liquid of chitin dilution at growing vegetables the last fortnight and to spill vegetable seedling, evenly be sprinkling upon after before growing vegetables, mixing thoroughly with the completely happy fertilizer of 20Kg-60Kg/ mu on the ground afterwards and carry out rotary tillage and soil with rotovator and fully mix thoroughly and carry out the field planting vegetables afterwards with the many granules of 10%% happy lot of 1.0Kg-2.0Kg/ mu.In order to consolidate control efficiency, after plantation one month, add water with biologic product and dilute the filling root.
Mostly said happy lot is that 10% fosthiazate granule of Japanese stone original production is a kind of efficient, low toxicity of non-fumigation type, the environment-friendly type nematocide of low-residual.Said biologic product is that microemulsion, Paecilomyces lilacinus are 200,000,000 spores/gram Paecilomyces lilacinus bacteria powder, kill a kind of in the production of Korea S BIG company of line pioneer 6% synergy sieve Ah pinane now for sieve's Ah pinane now.
Field trial research shows that method of the present invention has remarkable prevention effect, has guaranteed significant raising the output protection effect.
The present invention through adopt chitin, sieve's Ah pinane now, Paecilomyces lilacinus and kill among the line pioneer one or both and handle the seedbed, before the field planting with happy lot many with completely happy mixed processing soil, cooperate again after the field planting and use biologic product; Have easy to usely, safe and effective, can effectively control root knot nematode disease; Can promote the vegetable plant strain growth again; Have the fertile double effects of medicine, and can alleviate the environmental pollution that the heavy dose of use of agricultural chemicals is brought, early stage is through the nematode population in the low soil of chemical nematocide happy lot multiple pressure; Through the happy resistance that has increased vegetables of chitin and medicine grossness ground; The field planting later stage is regulated and control nematode population residual in the soil through biologic product, has reached the effect of biological-chemical Collaborative Control root-knot nematode disease, has very high promotional value.
Embodiment
Embodiment 1
Choosing the multiple plot of root knot nematode disease of going in in a big way proving ground, big eastern plantation center in Beijing implements.
Crop is a watermelon
Kind is beautiful No. 2 of capital
The field planting time: on February 22nd, 2008
Uproot plants after their edible portions have been harvested the time (control time): on June 2nd, 2008
The practical implementation method is following:
Watermelon field planting the last fortnight evenly spills with 500 times of dilutions of chitin and executes seedling-cultivating tray; Watermelon field planting the last week with sieve's Ah pinane now 1000 times of dilutions evenly spread fertilizer over the fields, before the watermelon field planting same day with the many 1.5Kg of happy lot and 40Kg is completely happy mix after, evenly be sprinkling upon soil surface; Carry out afterwards field planting 2 times with the rotovator rotary tillage; With the dosage of pressing 20mL/100 seedling now with sieve's Ah pinane, 2000 times of thin ups are irritated root after one month in field planting.When watermelon is uprooted plants after their edible portions have been harvested, investigate and contrast root and tie index, calculate control efficiency (seeing table 1).The result shows that the present invention is very obvious to the control efficiency of watermelon root-knot nematode disease, and its control efficiency can reach 92.69%.
The control efficiency of the collaborative prevention and control watermelon root-knot nematode of table 1 biological-chemical
| Handle | Root knot index | Control efficiency (%) |
| Contrast (not having any operation) | 59.48 | ? |
| Embodiment 1 | 4.35 | 92.69 |
Embodiment 2
Choosing the multiple plot of root knot nematode disease that Beijing's Shunyi District carries over special vegetable base plantation proving ground, center implements.
Crop is a muskmelon
Kind is white meteor
The field planting time: on February 13rd, 2008
Uproot plants after their edible portions have been harvested the time (control time): on June 16th, 2008
The practical implementation method is following:
The last week is evenly spread fertilizer over the fields seedling-cultivating tray with 500 times of dilutions of chitin in the muskmelon field planting; After mixed with the many 1.5Kg of happy lot and 60Kg are completely happy the same day before the muskmelon field planting; Evenly be sprinkling upon soil surface, with carrying out field planting after the rotovator rotary tillage 2 times, the dosage of 15mL/100 seedling is pressed in field planting now with sieve's Ah pinane after one month; 1500 times of thin ups are irritated root.When muskmelon is uprooted plants after their edible portions have been harvested, investigate and contrast root and tie index, calculate control efficiency (seeing table 2).The result shows that the present invention is very obvious to the control efficiency of sweet root-knot nematode disease, and its control efficiency can reach 88.57%.
The control efficiency of the collaborative prevention and control muskmelon root-knot nematode of table 2 biological-chemical
| Handle | Root knot index | Control efficiency (%) |
| Contrast (not having any operation) | 64.81 | ? |
| Embodiment 2 | 7.41 | 88.57 |
Embodiment 3
Choosing the multiple plot of root knot nematode disease of going in in a big way proving ground, big eastern plantation center in Beijing implements.
Crop is a watermelon
Kind is beautiful No. 2 of capital
The field planting time: on February 22nd, 2008
Uproot plants after their edible portions have been harvested the time (control time): on June 2nd, 2008
The practical implementation method is following:
Watermelon field planting the last week with sieve's Ah pinane now 1000 times of dilutions evenly spread fertilizer over the fields seedling-cultivating tray; Preceding 1 day of watermelon field planting with happy lot many 2Kg and 40Kg is completely happy mix after; Evenly be sprinkling upon soil surface, with carrying out field planting after the rotovator rotary tillage 2 times, the dosage of 2Kg/ mu is pressed in field planting with Paecilomyces lilacinus after one month; 1000 times of thin ups are irritated root.When watermelon is uprooted plants after their edible portions have been harvested, investigate and contrast root and tie index, calculate control efficiency (seeing table 3).The result shows that the present invention is very obvious to the control efficiency of watermelon root-knot nematode disease, and its control efficiency can reach 92.32%.
The control efficiency of the collaborative prevention and control watermelon root knot nematode disease of table 3 biological-chemical
| Handle | Root knot index | Control efficiency (%) |
| Contrast (not having any operation) | 48.15 | ? |
| Embodiment 3 | 3.70 | 92.32 |
Embodiment 4
Choosing the multiple plot of root knot nematode disease of green a kind of jade plantation proving ground, center, Mentougou, Beijing City district implements.
Crop is a cucumber
Kind is Beijing 102
The field planting time: on March 13rd, 2008
Uproot plants after their edible portions have been harvested the time (control time): on July 2nd, 2008
The practical implementation method is following:
The last week is evenly spread fertilizer over the fields seedling-cultivating tray with 500 times of dilutions of chitin in the cucumber field planting; After mixed with the many 2Kg of happy lot and 20Kg are completely happy the same day before the cucumber field planting; Evenly be sprinkling upon soil surface, carry out field planting afterwards 2 times with the rotovator rotary tillage, field planting is used after one month and is killed the dosage that the line pioneer presses 500mL/ mu; 2000 times of thin ups are irritated root.When cucumber is uprooted plants after their edible portions have been harvested, investigate and contrast root and tie index, calculate control efficiency (seeing table 4).The result shows that the present invention is very obvious to the control efficiency of cucumber root knot nematode disease evil, and its control efficiency can reach 94.82%.
The control efficiency of the collaborative prevention and control cucumber root-knot nematode of table 4 biological-chemical
| Handle | Root knot index | Control efficiency (%) |
| Contrast (not having any operation) | 100 | ? |
| Embodiment 4 | 5.18 | 94.82 |
Embodiment 5
Choosing the multiple plot of root knot nematode disease of green a kind of jade plantation proving ground, center, Mentougou, Beijing City district implements.
Crop is a tomato
Kind is cooperation 918
The field planting time: on March 1st, 2008
Uproot plants after their edible portions have been harvested the time (control time): on July 18th, 2008
The practical implementation method is following:
The last week is evenly spread fertilizer over the fields seedling-cultivating tray with 1000 times of dilutions of Paecilomyces lilacinus in the tomato field planting; After tomato field planting same day, many 1Kg mixed with completely happy 60Kg with happy lot; Evenly be sprinkling upon soil surface, with carrying out field planting after the rotovator rotary tillage 2 times, the dosage of 2Kg/ mu is pressed in field planting with chitin after one month; 1000 times of thin ups are irritated root.When tomato is uprooted plants after their edible portions have been harvested, investigate and contrast root and tie index, calculate control efficiency (seeing table 5).The result shows that the present invention is very obvious to the control efficiency of tomato root-knot eelworm disease, and its control efficiency can reach 100%.
The sick control efficiency of the collaborative prevention and control tomato root-knot eelworm of table 5 biological-chemical
| Handle | Root knot index | Control efficiency (%) |
| Contrast (not having any operation) | 33.33 | ? |
| Embodiment 5 | 0 | 100 |
Embodiment 6
Choosing the multiple plot of root knot nematode disease of green a kind of jade plantation proving ground, center, Mentougou, Beijing City district implements.
Crop is a tomato
Kind is cooperation 918
The field planting time: on March 1st, 2008
Uproot plants after their edible portions have been harvested the time (control time): on July 18th, 2008
The practical implementation method is following:
The last week is evenly spread fertilizer over the fields seedling-cultivating tray with killing 2000 times of dilutions of line pioneer in the tomato field planting; After tomato field planting same day, many 1.2Kg mixed with completely happy 48Kg with happy lot; Evenly be sprinkling upon soil surface, with carrying out field planting after the rotovator rotary tillage 2 times, the dosage of 20mL/100 seedling is pressed in field planting now with sieve's Ah pinane after one month; 1500 times of thin ups are irritated root.When tomato is uprooted plants after their edible portions have been harvested, investigate and contrast root and tie index, calculate control efficiency (seeing table 6).The result shows that the present invention is very obvious to the control efficiency of tomato root-knot eelworm disease, and its control efficiency can reach 100%.
The sick control efficiency of the collaborative prevention and control tomato root-knot eelworm of table 6 biological-chemical
| Handle | Root knot index | Control efficiency (%) |
| Contrast (not having any operation) | 41.59 | ? |
| Embodiment 6 | 0 | 100 |
Embodiment 7
Choosing the multiple plot of root knot nematode disease that Beijing's Shunyi District carries over special vegetable base plantation proving ground, center implements.
Crop is a watermelon
Kind is the capital show
The field planting time: on January 30th, 2008
Uproot plants after their edible portions have been harvested the time (control time): on June 16th, 2008
The practical implementation method is following:
Watermelon field planting the last fortnight evenly spills with 500 times of dilutions of chitin and executes seedling-cultivating tray; Evenly spread fertilizer over the fields with 1000 times of dilutions of Paecilomyces lilacinus the last week in the watermelon field planting, after mixed with the many 1.5Kg of happy lot and 40Kg are completely happy the same day before the watermelon field planting, evenly be sprinkling upon soil surface; Carry out afterwards field planting 2 times with the rotovator rotary tillage; With the dosage of pressing 10mL/100 seedling now with sieve's Ah pinane, 2000 times of thin ups are irritated root after one month in field planting.The result shows that the present invention is very obvious to the control efficiency of watermelon root-knot nematode disease, and its control efficiency can reach 95.48%.
The control efficiency of the collaborative prevention and control watermelon root knot nematode disease of table 7 biological-chemical
| Handle | Root knot index | Control efficiency (%) |
| Contrast (not having any operation) | 100 | ? |
| Embodiment 7 | 4.52 | 95.48 |
Claims (5)
1 A method of disease prevention and control root-knot nematode, which is characterized in 1-2 weeks before planting crops with chitin, hereby Arrow pinene, Paecilomyces lilacinus and kill lines in one or both of the pioneers to handle seedbed; before planting the crop with 1.0Kg-2.0Kg / acre more than 10% of blessing granules and 20Kg-60Kg / acre Montreal Le fertilizer mixture, evenly spread on the ground, with a rotary machine rotary and soil stir well to carry out soil after transplanting; month after planting with chitin, hereby Arrow pinene, Paecilomyces lilacinus and kill any one line of Pioneer IRRIGATING diluted with water; used to protect cultivation of vegetables.
2. the method for claim 1 is characterized in that: 500 times of chitin thin ups, filling root.
3 as claimed in claim 1, characterized in that: Arrow efficiency of 6% pinene hereby arrow pinene hereby microemulsion diluted 500-fold with water and pouring.
4. the method for claim 1 is characterized in that: 2000 times in Paecilomyces lilacinus 200,000,000 spores/gram Paecilomyces lilacinus bacteria powder thin up, irritate root.
5. the method for claim 1 is characterized in that: kill the line pioneer, 2000 times of thin ups, filling root.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008101882672A CN101422168B (en) | 2008-12-23 | 2008-12-23 | New method for controlling rot knot nematode disease of protected vegetable |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101882672A CN101422168B (en) | 2008-12-23 | 2008-12-23 | New method for controlling rot knot nematode disease of protected vegetable |
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| CN101422168A CN101422168A (en) | 2009-05-06 |
| CN101422168B true CN101422168B (en) | 2012-03-21 |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101671210B (en) * | 2009-10-29 | 2012-08-08 | 福建省农业科学院农业生物资源研究所 | Preparation method of paecilomyces lilacinus biological fertilizer |
| CN101904235B (en) * | 2010-07-22 | 2011-12-07 | 济南兆龙科技发展有限公司 | Special method for applying 98% of Longxin dazomet microgranule |
| MX364353B (en) * | 2011-04-29 | 2017-06-13 | Inst De Ecologia A C Star | Uses, methods and biological compositions of the paecilomyces genus for the control, prevention and eradication of phytoparasites in solanaceae crops. |
| CN102422844B (en) * | 2011-09-27 | 2013-04-10 | 德强生物股份有限公司 | Paecilomyces lilacinus-containing composition for preventing and controlling nematodes |
| CN105007741A (en) * | 2012-12-03 | 2015-10-28 | 拜耳作物科学股份公司 | Composition comprising a biological control agent and a fungicide |
| MX360582B (en) | 2012-12-13 | 2018-11-07 | Inst De Ecologia A C Star | Biocontrol of phyto-parasitic nematodes using paecilomyces. |
| CN103503924B (en) * | 2013-08-29 | 2015-05-06 | 中国农业科学院蔬菜花卉研究所 | Preparation method and applications of fusarium oxysporum inoculant |
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| CN104705128A (en) * | 2015-03-12 | 2015-06-17 | 陈争峰 | Integrated prevention and treatment method for root-knot nematode in Eastern China |
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| CN105494448A (en) * | 2016-01-07 | 2016-04-20 | 山东亿丰源生物科技股份有限公司 | Preparation method of biological preparation for preparing and curing vegetable nematode disease |
| CN105660711B (en) * | 2016-04-01 | 2018-08-07 | 青岛正道药业有限公司 | A kind of nematode granule with slow seedling effect |
| CN111448925A (en) * | 2020-04-08 | 2020-07-28 | 赤峰学院 | Method for preventing and treating root knot nematode disease of fruit and vegetable vegetables |
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2008
- 2008-12-23 CN CN2008101882672A patent/CN101422168B/en not_active Expired - Fee Related
Non-Patent Citations (6)
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| 冯艳梅.苦豆子提取物及其系列物防治番茄和黄瓜根结线虫病初探.《中国优秀硕士学位论文全文数据库》.2006,(第1期),第20页第1-3段. * |
| 姚红燕.宁波地区番茄根结线虫病初探.《宁波农业科技》.2008,(第4期),第5-7页. * |
| 孔凡彬等.茄子根结线虫发生规律及综合防治技术.《安徽农业科学》.2005,第33卷(第3期),第446页. * |
| 李宝聚等.蔬菜根结线虫的发生与日光土壤消毒技术.《中国蔬菜》.2006,(第5期),第49-50页. * |
| 郑永利等.10 %福气多颗粒剂防治芹菜根结线虫药效试验.《中国蔬菜》.2006,(第5期),第24-25页. * |
| 鲁秀芝等.日光温室蔬菜根结线虫病的发生与防治.《内蒙古农业科技》.2007,(第6期),第122-123页. * |
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| Publication number | Publication date |
|---|---|
| CN101422168A (en) | 2009-05-06 |
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