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CN101422168A - New method for controlling rot knot nematode disease of protected vegetable - Google Patents

New method for controlling rot knot nematode disease of protected vegetable Download PDF

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Publication number
CN101422168A
CN101422168A CNA2008101882672A CN200810188267A CN101422168A CN 101422168 A CN101422168 A CN 101422168A CN A2008101882672 A CNA2008101882672 A CN A2008101882672A CN 200810188267 A CN200810188267 A CN 200810188267A CN 101422168 A CN101422168 A CN 101422168A
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China
Prior art keywords
root
field planting
knot nematode
chitin
paecilomyces lilacinus
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CNA2008101882672A
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Chinese (zh)
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CN101422168B (en
Inventor
刘霆
刘伟成
刘建华
裘季燕
卢彩鸽
刘德文
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Beijing Academy of Agriculture and Forestry Sciences
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Beijing Academy of Agriculture and Forestry Sciences
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Abstract

The invention discloses a new method for controlling vegetable root knot nematode, which is characterized in that one or two from chitin, aropaz, Paecilomyces lilacinus and nematicidal pioneer is/are selected to process a seedbed in 1 to 2 weeks before the field planting of vegetables; 1.0Kg to 2.0Kg /mu (1 acre equals to 6.07 mu) of Fosthiazate and 20Kg to 60Kg/mu of harmonia mundi are mixed to process soil before the field planting; after the field planting, a biological agent is matched and used; the method is convenient to be used, is safe and effective, not only can effectively control the root knot nematode, but also can promote the growing of the vegetable plants, has the double effects of pesticide and fertilizer and can lighten the environment pollution brought by the use of the pesticide in large dosage; in the early period, the numbers of the eelworms in the soil are reduced by the mechanical eelworm pesticide of Fosthiazate; the stress resistance of the vegetables are increased by the chitin and the pesticide-fertilizer harmonia mundi; during the later period of the field planting, the numbers of the eelworms remained in the soil are controlled by using any of the biological agent of chitin, the aropaz, the Paecilomyces lilacinus and the nematicidal pioneer to irrigate the root, thus achieving the effect of controlling the vegetable root knot nematode by the cooperation of biology and chemistry and having very high popularization value.

Description

A kind of new method of facility for prevention and control root knot nematode disease evil
Technical field:
The present invention relates to a kind of new method of facility for prevention and control root knot nematode disease evil.
Background technology:
The many in the world countries and regions of plant nematode take place and cause harm, and kind is varied.In the world, the generation of plant nematode and harm are along with the variation of the development of agriculture and forestry and tillage and cultivation system is serious day by day, add up according to Esser, the whole world has been reported and has been found that plant nematode 207 belongs to 4832 kinds of (Liu Weizhi till the nineteen ninety, Duan Yuxi .2000. plant pathogeny line insect is learned. Beijing: and Chinese agriculture publishing house, p1-2).According to estimates, it is 12.3% that plant nematode is caused the year percent of loss of world staple crops, above 1,000 hundred million dollars, and actual loss is considerably beyond estimation, reason be the harm that causes of many nematodes because of do not have visible symptom in field or specialization feature do not arouse attention (Wang Shouhua. the fruit tree nematology. Beijing: Chinese agriculture science and technology publishing house, 1994.P.1-5), in addition, the new discovery of in the world wide nematode being caused harm is also in continuous increase, and the agricultural cultivation system of some renewals makes the nematode problem more outstanding, moreover nematode and other biological interaction and direct or indirect influence that plant is produced are also in rapid increase.The harm that nematode is caused is more hidden than other biology in this sense! At present the harmful nematode of plant there is kind more than 3000 approximately, mainly contains root-knot nematode, Cyst nematode, pine wood nematode, sweet potato stem nematode etc. in China.Root-knot nematode is the maximum class nematode of causing harm on the agricultural, after disease takes place, the general underproduction about 10%, serious in more than 75%, even total crop failure (A.G.Whitehead, 1998.Plant Nematode Control.ISBN0851991882 CABInternational).The root-knot nematode that works the mischief in China mainly comprises 4 kinds, i.e. Meloidogyne incognita (Meloidogyneincognita), peanut root-knot nematode (M.arenaria), northern root-knot nematode (M.hapla) and javanese root knot nematode (M.javarnica).Root-knot nematode is infected root system of plant with the form of larva, the ensuing crop underproduction significantly of being injured, even total crop failure, and the generation of isochrone parasitosis has aggravated the harm of soil-borne diseases such as fusarium wilt, verticillium wilt, damping off again.
Control to root-knot nematode at present lacks practicality and high efficiency and continuable improvement technology, and chemical nematode killing agent can only be controlled nematode population in a short time, still has cost height, potentially contaminated, holds problems such as imitating weak point, pesticide resistance.Reasonably cultivating system can play certain preventive and therapeutic effect; but crop and resistant variety that can crop rotation in protection ground be limited; therefore, the harm of root-knot nematode has become the outstanding problem of restriction pollution-free vegetable production sustainable development and the guarantee of capital food security.Efficient, the practical and continuable root-knot nematode Prevention Technique system of research is to produce at present to go up the key technology of being badly in need of.
Summary of the invention:
The facility for prevention and control root knot nematode disease evil new method that the purpose of this invention is to provide a kind of efficient, low toxicity, low-residual.
For achieving the above object, the present invention adopts following technical scheme: a kind of new method of facility for prevention and control root knot nematode disease evil, this method has overcome the single chemical method of traditional usefulness and has prevented and treated the shortcoming of root-knot nematode and a kind of chemical agent (happy lot is many) and bio-fertilizer (chitin are provided, completely happy) and biopesticide (sieve's Ah pinane is microemulsion now, Paecilomyces lilacinus, kill the line pioneer) low toxicity that combines, efficiently, the method of preventing and treating of safety, concrete grammar is mainly caught seedling stage, setting date and vegetative period three controls periods, carry out and grow seedlings, whole ground, field planting, 4 key links such as field management.Evenly water with 500 times of liquid of chitin dilution at growing vegetables the last fortnight and to spill vegetable seedling, evenly be sprinkling upon after before growing vegetables, mixing thoroughly with the completely happy fertilizer of 20Kg-60Kg/ mu on the ground afterwards and carry out rotary tillage and soil with rotovator and fully mix thoroughly and carry out the field planting vegetables afterwards with the many granules of 10%% happy lot of 1.0Kg-2.0Kg/ mu.In order to consolidate control efficiency, after plantation one month, add water with biologic product and dilute the filling root.
The 10% fosthiazate granule that described happy lot mostly is Japanese stone original production is a kind of efficient, low toxicity of non-fumigation type, the environment-friendly type nematocide of low-residual.Described biologic product is that microemulsion, Paecilomyces lilacinus are 200,000,000 spores/gram Paecilomyces lilacinus bacteria powder, kill a kind of in the production of Korea S BIG company of line pioneer 6% synergy sieve Ah pinane now for sieve's Ah pinane now.
Field trial studies show that method of the present invention has remarkable prevention effect, has guaranteed significant volume increase protection effect.
The present invention is by adopting chitin, sieve's Ah pinane Hereby, Paecilomyces lilacinus and kill among the line pioneer one or both and handle the seedbed, before the field planting with happy lot many with completely happy mixed processing soil, cooperate again after the field planting and use biologic product, have easy to use, safe and effective, can effectively control root knot nematode disease, can promote the vegetable plant strain growth again, has the fertile double effects of medicine, and can alleviate the environmental pollution that the heavy dose of use of agricultural chemicals is brought, early stage is by the nematode population in the low soil of chemical nematocide happy lot multiple pressure, by the happy resistance that has increased vegetables of chitin and medicine grossness ground, the field planting later stage is regulated and control nematode population residual in the soil by biologic product, has reached the effect of biological-chemical cooperated control root-knot nematode disease, has very high promotional value.
Embodiment
Embodiment 1
Choosing the multiple plot of root knot nematode disease of going in in a big way proving ground, big eastern plantation center in Beijing implements.
Crop is a watermelon
Kind is beautiful No. 2 of capital
The field planting time: on February 22nd, 2008
Uproot plants after their edible portions have been harvested the time (control time): on June 2nd, 2008
Specific implementation method is as follows:
Watermelon field planting the last fortnight evenly spills with 500 times of dilutions of chitin and executes seedling-cultivating tray, evenly spread fertilizer over the fields with 1000 times of dilutions of sieve's Ah pinane Hereby the last week in the watermelon field planting, after mixed with the many 1.5Kg of happy lot and 40Kg are completely happy the same day before the watermelon field planting, evenly be sprinkling upon soil surface, carry out afterwards field planting 2 times with the rotovator rotary tillage, with the dosage of pressing 20mL/100 seedling with sieve's Ah pinane Hereby, 2000 times of thin ups are irritated root after one month in field planting.When watermelon is uprooted plants after their edible portions have been harvested, investigate and contrast the root knot index, calculate control efficiency (seeing Table 1).The result shows that the present invention is very obvious to the control efficiency of watermelon root-knot nematode disease, and its control efficiency can reach 92.69%.
The control efficiency of table 1 biology-chemical cooperated prevention and control watermelon root-knot nematode
Handle The root knot index Control efficiency (%)
Contrast (not having any operation) 59.48
Embodiment 1 4.35 92.69
Embodiment 2
Choosing the multiple plot of root knot nematode disease that Beijing's Shunyi District carries over special vegetable base plantation proving ground, center implements.
Crop is a muskmelon
Kind is white meteor
The field planting time: on February 13rd, 2008
Uproot plants after their edible portions have been harvested the time (control time): on June 16th, 2008
Specific implementation method is as follows:
The last week is evenly spread fertilizer over the fields seedling-cultivating tray with 500 times of dilutions of chitin in the muskmelon field planting, after mixed with the many 1.5Kg of happy lot and 60Kg are completely happy the same day before the muskmelon field planting, evenly be sprinkling upon soil surface, carry out afterwards field planting 2 times with the rotovator rotary tillage, the dosage that field planting was pressed 15mL/100 seedling with sieve's Ah pinane Hereby after one month, 1500 times of thin ups are irritated root.When muskmelon is uprooted plants after their edible portions have been harvested, investigate and contrast the root knot index, calculate control efficiency (seeing Table 2).The result shows that the present invention is very obvious to the control efficiency of sweet root-knot nematode disease, and its control efficiency can reach 88.57%.
The control efficiency of table 2 biology-chemical cooperated prevention and control muskmelon root-knot nematode
Handle The root knot index Control efficiency (%)
Contrast (not having any operation) 64.81
Embodiment 2 7.41 88.57
Embodiment 3
Choosing the multiple plot of root knot nematode disease of going in in a big way proving ground, big eastern plantation center in Beijing implements.
Crop is a watermelon
Kind is beautiful No. 2 of capital
The field planting time: on February 22nd, 2008
Uproot plants after their edible portions have been harvested the time (control time): on June 2nd, 2008
Specific implementation method is as follows:
The last week is evenly spread fertilizer over the fields seedling-cultivating tray with 1000 times of dilutions of sieve's Ah pinane Hereby in the watermelon field planting, preceding 1 day of watermelon field planting with happy lot many 2Kg and 40Kg is completely happy mix after, evenly be sprinkling upon soil surface, carry out afterwards field planting 2 times with the rotovator rotary tillage, the dosage of 2Kg/ mu is pressed in field planting with Paecilomyces lilacinus after one month, 1000 times of thin ups are irritated root.When watermelon is uprooted plants after their edible portions have been harvested, investigate and contrast the root knot index, calculate control efficiency (seeing Table 3).The result shows that the present invention is very obvious to the control efficiency of watermelon root-knot nematode disease, and its control efficiency can reach 92.32%.
The control efficiency of table 3 biology-chemical cooperated prevention and control watermelon root knot nematode disease
Handle The root knot index Control efficiency (%)
Contrast (not having any operation) 48.15
Embodiment 3 3.70 92.32
Embodiment 4
Choosing the multiple plot of root knot nematode disease of green a kind of jade plantation proving ground, center, Mentougou, Beijing City district implements.
Crop is a cucumber
Kind is Beijing 102
The field planting time: on March 13rd, 2008
Uproot plants after their edible portions have been harvested the time (control time): on July 2nd, 2008
Specific implementation method is as follows:
The last week is evenly spread fertilizer over the fields seedling-cultivating tray with 500 times of dilutions of chitin in the cucumber field planting, after mixed with the many 2Kg of happy lot and 20Kg are completely happy the same day before the cucumber field planting, evenly be sprinkling upon soil surface, carry out afterwards field planting 2 times with the rotovator rotary tillage, field planting is used after one month and is killed the dosage that the line pioneer presses 500mL/ mu, 2000 times of thin ups are irritated root.When cucumber is uprooted plants after their edible portions have been harvested, investigate and contrast the root knot index, calculate control efficiency (seeing Table 4).The result shows that the present invention is very obvious to the control efficiency of cucumber root knot nematode disease evil, and its control efficiency can reach 94.82%.
The control efficiency of table 4 biology-chemical cooperated prevention and control cucumber root-knot nematode
Handle The root knot index Control efficiency (%)
Contrast (not having any operation) 100
Embodiment 4 5.18 94.82
Embodiment 5
Choosing the multiple plot of root knot nematode disease of green a kind of jade plantation proving ground, center, Mentougou, Beijing City district implements.
Crop is a tomato
Kind is cooperation 918
The field planting time: on March 1st, 2008
Uproot plants after their edible portions have been harvested the time (control time): on July 18th, 2008
Specific implementation method is as follows:
The last week is evenly spread fertilizer over the fields seedling-cultivating tray with 1000 times of dilutions of Paecilomyces lilacinus in the tomato field planting, after tomato field planting same day, many 1Kg mixed with completely happy 60Kg with happy lot, evenly be sprinkling upon soil surface, carry out afterwards field planting 2 times with the rotovator rotary tillage, the dosage of 2Kg/ mu is pressed in field planting with chitin after one month, 1000 times of thin ups are irritated root.When tomato is uprooted plants after their edible portions have been harvested, investigate and contrast the root knot index, calculate control efficiency (seeing Table 5).The result shows that the present invention is very obvious to the control efficiency of tomato root-knot eelworm disease, and its control efficiency can reach 100%.
The control efficiency of table 5 biology-chemical cooperated prevention and control tomato root-knot eelworm disease
Handle The root knot index Control efficiency (%)
Contrast (not having any operation) 33.33
Embodiment 5 0 100
Embodiment 6
Choosing the multiple plot of root knot nematode disease of green a kind of jade plantation proving ground, center, Mentougou, Beijing City district implements.
Crop is a tomato
Kind is cooperation 918
The field planting time: on March 1st, 2008
Uproot plants after their edible portions have been harvested the time (control time): on July 18th, 2008
Specific implementation method is as follows:
The last week is evenly spread fertilizer over the fields seedling-cultivating tray with killing 2000 times of dilutions of line pioneer in the tomato field planting, after tomato field planting same day, many 1.2Kg mixed with completely happy 48Kg with happy lot, evenly be sprinkling upon soil surface, carry out afterwards field planting 2 times with the rotovator rotary tillage, the dosage that field planting was pressed 20mL/100 seedling with sieve's Ah pinane Hereby after one month, 1500 times of thin ups are irritated root.When tomato is uprooted plants after their edible portions have been harvested, investigate and contrast the root knot index, calculate control efficiency (seeing Table 6).The result shows that the present invention is very obvious to the control efficiency of tomato root-knot eelworm disease, and its control efficiency can reach 100%.
The control efficiency of table 6 biology-chemical cooperated prevention and control tomato root-knot eelworm disease
Handle The root knot index Control efficiency (%)
Contrast (not having any operation) 41.59
Embodiment 6 0 100
Embodiment 7
Choosing the multiple plot of root knot nematode disease that Beijing's Shunyi District carries over special vegetable base plantation proving ground, center implements.
Crop is a watermelon
Kind is the capital show
The field planting time: on January 30th, 2008
Uproot plants after their edible portions have been harvested the time (control time): on June 16th, 2008
Specific implementation method is as follows:
Watermelon field planting the last fortnight evenly spills with 500 times of dilutions of chitin and executes seedling-cultivating tray, evenly spread fertilizer over the fields with 1000 times of dilutions of Paecilomyces lilacinus the last week in the watermelon field planting, after mixed with the many 1.5Kg of happy lot and 40Kg are completely happy the same day before the watermelon field planting, evenly be sprinkling upon soil surface, carry out afterwards field planting 2 times with the rotovator rotary tillage, with the dosage of pressing 10mL/100 seedling with sieve's Ah pinane Hereby, 2000 times of thin ups are irritated root after one month in field planting.The result shows that the present invention is very obvious to the control efficiency of watermelon root-knot nematode disease, and its control efficiency can reach 95.48%.
The control efficiency of table 7 biology-chemical cooperated prevention and control watermelon root knot nematode disease
Handle The root knot index Control efficiency (%)
Contrast (not having any operation) 100
Embodiment 7 4.52 95.48

Claims (10)

1, a kind of method of preventing and treating the root-knot nematode disease, it is characterized in that before crop field planting 1-2 week with chitin, sieve's Ah pinane Hereby, Paecilomyces lilacinus and extremely one or both among the line pioneer handle the seedbed.
2, a kind of method of preventing and treating root knot nematode disease as claimed in claim 1 is carried out rotary tillage and soil with rotovator after evenly being sprinkling upon on the ground after it is characterized in that before the crop field planting mixing thoroughly with the many granules of 10% happy lot of 1.0Kg-2.0Kg/ mu and the completely happy fertilizer of 20Kg-60Kg/ mu and is fully mixed thoroughly and handle soil and carry out transplant planting afterwards.
3, a kind of new method of preventing and treating root knot nematode disease as claimed in claim 2 is characterized in that after plantation one month, and usefulness chitin, sieve's Ah pinane Hereby, Paecilomyces lilacinus and any thin up that kills among the line pioneer are irritated root.
4, according to claim 1,2 or 3 described methods, it is characterized in that: described vegetables are the vegetables of protection ground cultivation.
5, the method for claim 1 is characterized in that: 500 times of chitin thin ups, irritate root.
6, the method for claim 1 is characterized in that: sieve's Ah pinane Hereby is 500 times of 6% synergy sieve Ah pinane Hereby microemulsion thin ups, irritates root.
7, the method for claim 1 is characterized in that: 2000 times in Paecilomyces lilacinus 200,000,000 spores/gram Paecilomyces lilacinus bacteria powder thin up, irritate root.
8, the method for claim 1 is characterized in that: kill the line pioneer, 2000 times of thin ups are irritated root.
9, method as claimed in claim 2 is characterized in that: happy lot mostly is 10% fosthiazate granule, mu consumption 1.0Kg-2.0Kg/ mu.
10, method as claimed in claim 2 is characterized in that: completely happy for having the organic manure of nematocidal effect, mu consumption 20-60Kg/ mu.
CN2008101882672A 2008-12-23 2008-12-23 New method for controlling rot knot nematode disease of protected vegetable Expired - Fee Related CN101422168B (en)

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Cited By (13)

* Cited by examiner, † Cited by third party
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CN101904235A (en) * 2010-07-22 2010-12-08 济南兆龙科技发展有限公司 Special method for applying 98% of Longxin dazomet microgranule
CN102422844A (en) * 2011-09-27 2012-04-25 德强生物股份有限公司 Nematode control composition containing paecilomyces lilacinus
CN101671210B (en) * 2009-10-29 2012-08-08 福建省农业科学院农业生物资源研究所 Preparation method of paecilomyces lilacinus biological fertilizer
WO2012148251A2 (en) 2011-04-29 2012-11-01 Instituto De Ecologia, A.C. Uses, methods and biological compositions of the genus paecilomyces for the control, prevention and eradication of phytoparasites in solanaceae cultures
CN103503924A (en) * 2013-08-29 2014-01-15 中国农业科学院蔬菜花卉研究所 Preparation method and applications of fusarium oxysporum inoculant
WO2014092529A1 (en) 2012-12-13 2014-06-19 Instituto De Ecología, A.C. Biocontrol of phyto-parasitic nematodes using paecilomyces
CN103875706A (en) * 2014-03-20 2014-06-25 黄业新 Pesticide granules
CN104705128A (en) * 2015-03-12 2015-06-17 陈争峰 Integrated prevention and treatment method for root-knot nematode in Eastern China
CN105007741A (en) * 2012-12-03 2015-10-28 拜耳作物科学股份公司 Composition comprising a biological control agent and a fungicide
CN105112070A (en) * 2015-09-15 2015-12-02 北京好收成全农生物科技有限公司 Acid soil conditioner and preparation method thereof
CN105494448A (en) * 2016-01-07 2016-04-20 山东亿丰源生物科技股份有限公司 Preparation method of biological preparation for preparing and curing vegetable nematode disease
CN105660711A (en) * 2016-04-01 2016-06-15 广西南宁益土生物科技有限责任公司 Nematode granules with seedling recovering effect
CN111448925A (en) * 2020-04-08 2020-07-28 赤峰学院 Method for preventing and treating root knot nematode disease of fruit and vegetable vegetables

Cited By (21)

* Cited by examiner, † Cited by third party
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CN101671210B (en) * 2009-10-29 2012-08-08 福建省农业科学院农业生物资源研究所 Preparation method of paecilomyces lilacinus biological fertilizer
CN101904235A (en) * 2010-07-22 2010-12-08 济南兆龙科技发展有限公司 Special method for applying 98% of Longxin dazomet microgranule
WO2012148251A2 (en) 2011-04-29 2012-11-01 Instituto De Ecologia, A.C. Uses, methods and biological compositions of the genus paecilomyces for the control, prevention and eradication of phytoparasites in solanaceae cultures
US10070653B2 (en) 2011-04-29 2018-09-11 Instituto De Ecologia, A.C. Uses, methods and biological compositions of the genus Paecilomyces in the control, prevention and eradication of plant parasites in Solanaceae cultures
RU2636550C2 (en) * 2011-04-29 2017-11-23 Институто Де Эколохия, А.С. Bio-nematocidal composition for prevention, destruction or fighting of nematodes in nightshade family cultures and its application
CN102422844A (en) * 2011-09-27 2012-04-25 德强生物股份有限公司 Nematode control composition containing paecilomyces lilacinus
CN102422844B (en) * 2011-09-27 2013-04-10 德强生物股份有限公司 Paecilomyces lilacinus-containing composition for preventing and controlling nematodes
CN105007741A (en) * 2012-12-03 2015-10-28 拜耳作物科学股份公司 Composition comprising a biological control agent and a fungicide
WO2014092529A1 (en) 2012-12-13 2014-06-19 Instituto De Ecología, A.C. Biocontrol of phyto-parasitic nematodes using paecilomyces
US9867378B2 (en) 2012-12-13 2018-01-16 Instituto De Ecologia, A.C. Biocontrol of phytoparasitic nematodes by paecilomyces
CN103503924A (en) * 2013-08-29 2014-01-15 中国农业科学院蔬菜花卉研究所 Preparation method and applications of fusarium oxysporum inoculant
CN103503924B (en) * 2013-08-29 2015-05-06 中国农业科学院蔬菜花卉研究所 Preparation method and applications of fusarium oxysporum inoculant
CN103875706B (en) * 2014-03-20 2015-09-30 陈光敏 A kind of granular pesticide
CN103875706A (en) * 2014-03-20 2014-06-25 黄业新 Pesticide granules
CN104705128A (en) * 2015-03-12 2015-06-17 陈争峰 Integrated prevention and treatment method for root-knot nematode in Eastern China
CN105112070A (en) * 2015-09-15 2015-12-02 北京好收成全农生物科技有限公司 Acid soil conditioner and preparation method thereof
CN105112070B (en) * 2015-09-15 2018-02-13 北京好收成全农生物科技有限公司 A kind of acidic soil conditioner and preparation method thereof
CN105494448A (en) * 2016-01-07 2016-04-20 山东亿丰源生物科技股份有限公司 Preparation method of biological preparation for preparing and curing vegetable nematode disease
CN105660711A (en) * 2016-04-01 2016-06-15 广西南宁益土生物科技有限责任公司 Nematode granules with seedling recovering effect
CN105660711B (en) * 2016-04-01 2018-08-07 青岛正道药业有限公司 A kind of nematode granule with slow seedling effect
CN111448925A (en) * 2020-04-08 2020-07-28 赤峰学院 Method for preventing and treating root knot nematode disease of fruit and vegetable vegetables

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