CN101401567A - Eremophyte steeping specimen and method for making protective color - Google Patents

Abstract

The invention discloses a method for making desert plant dipped specimens and color preservation, comprising: material selection and collection → pruning and cleaning → fixation solution immersion → preservation solution immersion → bottle sealing and labeling; the color retention fixation solution and the preservation solution The steeping step is aimed at the color system of the two major flowers, green and red, according to the different characteristics of the pigments contained in the plant organs. Continuous adjustment of chemical properties, through the color series screening of fixative solution and preservation solution, the acid-base balance is reasonably controlled, and the structure of pigment molecules in the plant becomes stable; the specimens treated with color retention can clearly show the plant body The morphological structure of the prepared plant dipped specimens is bright in color, strong in three-dimensionality, and lifelike in shape, which can perfectly display the subtle morphological differences of the flowers, fruits and leaves of plants, and enrich the research on specimen taxonomy and preservation methods. The present invention is aimed at the unique desert plants in Xinjiang. The method of soaking specimens with chemical liquid and preserving color all adopts glass ceramic vessels, and the chemical reagents are all conventional reagents. The cost is low, the method is highly operable, and the market prospect is promising.

Description

Preparation method of desert plant dipped specimen and color preservation

technical field

The invention relates to the production of plant specimens, especially for the production of dipped specimens and color retention, and is especially suitable for the production method of desert plant dipping specimens and color retention.

Background technique

The preservation of plant specimens has important practical value for botanical research and taxonomic research. There are two classic methods: one is the pressing method, that is, the whole plant or the branches with flowers and fruits are laid flat on absorbent paper, tightened with splints, and placed in a ventilated place to dry naturally. This method can be preserved in large quantities. specimens, but there are many problems, such as in the process of water absorption and drying, it is easy to deform the flowers and leaves of the plants and change the color, resulting in the loss and misleading of information closely related to plants and shapes; from the perspective of plant system classification, the information related to plants Completeness is particularly important for taxonomic identification. The difference between some species of plants lies in the subtle morphological differences of the flowers, fruits and leaves of plants. The second is to use the FAA fixative solution preservation method. Due to the effect of formaldehyde and glacial acetic acid, a polymerization reaction will occur after long-term storage, resulting in a white precipitate of paraformaldehyde, which makes the fixative solution turbid and cannot preserve the original color of the plant specimen. It is a thorny problem to keep the herbarium collections intact and free from mildew, discoloration and deterioration.

Regarding the production methods of plant dipping specimens and preservation of color and freshness, see “Experimental Research on Maintaining the Color of Original Plants of Traditional Chinese Medicine by Multi-color Killing and Fixing Method” by Comrade Liu Ruoyong in the Journal of Henan University of Traditional Chinese Medicine, Issue 4, July 2003. The author discloses experiments on Chinese medicine soaking specimens and color preservation, but the plant objects targeted by the present invention are different, because the slight imbalance of anthocyanin acid-base in the plant will change the flower color. Another "Plant Magazine" in 1996 Comrade Bian Yong's "Some Experiences of Dipping Plant Specimens" revealed the application experiment to the color retention of fruits in vegetables and fruits, but still differs from the plant objects targeted by the present invention. . Aiming at the unique desert plants in Xinjiang, the present invention uses the method of dipping specimens in chemical liquids, and conducts an impregnating liquid ratio experiment. Through comparison and screening, the best color-preserving liquid formula is obtained, and the plant specimens treated by color-preserving treatment can clearly express The morphological structure of the plant body, the prepared plant dipped specimens are bright in color, strong in three-dimensionality, and lifelike in shape, which can perfectly display the subtle morphological differences of the flowers, fruits and leaves of plants, and enrich the diversity research on specimen taxonomy and preservation methods.

Contents of the invention

The object of the present invention is to: provide a method for making desert plant dipped specimens, especially for the unique desert plants in Xinjiang, a dipping experiment has been carried out, and research on the dipping of the specimens by the dipping liquid and long-term preservation has been carried out, in order to obtain the preparation method of the dipped specimens. Scientific formula.

Another object of the present invention is: the formula can keep the dipping specimen in its original color and appearance, and under the classification microscope, the structure is clear, the cytoplasm and nucleus are well colored, and the color ratio is suitable and clear, which expands the range of desert plant specimens. taxonomic research.

The preparation method of desert plant dipped specimens and color preservation of the present invention, the preparation of dipped specimens includes: material selection and collection → pruning and cleaning → fixation liquid soaking → preservation liquid dipping → bottle sealing and labeling; the color preservation fixation liquid and preservation The soaking step of liquid is:

Immersion in pink flower fixative solution: use 1.8-4ml of 40% formalin, 2-4g boric acid, 20% alcohol 8-12ml and 800-1200ml water, soak for 22-26h; When the pigment or impurities are precipitated, transfer it to a preservation solution of 20-40ml of 6% sulfurous acid, 18-22g of boric acid and 900-1100ml of water for long-term storage;

Immersion in fixative solution for green leaves and small floral series: Use 5% copper sulfate solution to soak the specimen until it turns green→brown→yellow→green. The fixation time is 3-6d, remove and wash off the sulfuric acid For copper, bleach with sulfurous acid at a concentration of 2% to 3% for 1-2 days; when no pigment or impurities are precipitated, add 8-12ml of sulfurous acid with a concentration of 6%, 38-42ml of formaldehyde, 38-42ml of glycerin, and 900-1100ml of water. In the preservation solution, to be stored for a long time;

Soaking in fixed solution of red flowers: use 3-5ml concentration of 40% formalin, 2-4g boric acid and 380-420ml water, soak for 7-10d; -22ml of 10% sulfurous acid, 8-12g of boric acid and 900-1100ml of water in a preservation solution for long-term storage;

Immersion in yellow fixative solution: Use 38-42ml of 6% sulfurous acid and 800-1000ml of water to prepare the fixative solution, soak for 4-7d; when no pigment or impurities are precipitated, transfer to 23-27ml formalin , 23-27ml glycerin and 900-1100ml water preservation solution, to be stored for a long time;

Immersion in fixative solution for dark red and purple flowers: Soak in 20% magnesium chloride solution for 2-5 days, then soak in 30%, 50%, and 75% magnesium chloride solutions in sequence, each time for 4-8 days, and control the pH 7.5-9; then soaked in 85% magnesium chloride solution respectively, after soaking for 6-10 days each time, keep the original color in supersaturated magnesium chloride fixative solution; when no pigment or impurities are precipitated, move into 20% magnesium chloride solution storage solution for long-term storage.

The preparation method of soaking specimens and color preservation in the previous step, the best fixative solution and preservation solution soaking steps are as follows:

Immersion in pink flower fixative solution: use 2-3ml concentration of 40% formalin, 2-3g boric acid, concentration 20% alcohol 9-11ml and 900-1100ml water mixture, soak for 23-25h; When the pigment or impurities are precipitated, transfer it to a preservation solution of 25-35ml of 6% sulfurous acid, 19-21g of boric acid and 950-1050ml of water for long-term storage;

Immersion in fixative solution for green leaves and small floral series: Use 5% copper sulfate solution to soak the specimen until it turns green→brown→yellow→green. The fixation time is 3-5d, remove and wash off the sulfuric acid For copper, bleach with 2%-3% sulfurous acid for 1-1.5 days; when no pigment or impurities are precipitated, add 9-11ml of 6% sulfurous acid, 39-41ml of formaldehyde, 39-41ml of glycerin, and 950-1050ml of water in the storage solution for long-term storage;

Soaking in fixed solution of red flowers: use 3-5ml of 40% formalin, 2-4g of boric acid and 380-420ml of water, soak for 7-9d; -21ml of 10% sulfurous acid, 9-11g of boric acid and 950-1050ml of water in a preservation solution for long-term storage;

Immersion in yellow fixative solution: Use 39-41ml of 6% sulfurous acid and 850-950ml of water to prepare the fixative solution, soak for 4-6 days; when no pigment or impurities are precipitated, transfer to 24-26ml formalin , 24-26ml glycerin and 950-1050ml water preservation solution, to be stored for a long time;

Crimson and purple flowers are soaked in fixative solution: Soak in 20% magnesium chloride solution for 2-4 days, then soak in 30%, 50%, and 75% magnesium chloride solutions in sequence, each time for 5-7 days, and control the pH 7.8-8.5; and then soaked in 85% magnesium chloride solution respectively, after soaking for 7-9 days each time, keep the original color in supersaturated magnesium chloride fixative solution; when no pigment or impurities are precipitated, move into 20% magnesium chloride solution storage solution for long-term storage.

The preparation method of impregnating specimens and color preservation in the previous step, the best fixative and preservation solution immersion steps are:

Immersion of pink flower series fixative solution: use 2ml concentration of 40% formalin, 3g boric acid, concentration 20% alcohol 10ml and 1000ml water, soak for 24 hours; when no pigment or impurities are precipitated, transfer to 30ml concentration 6 % sulfurous acid, 20g boric acid and 1000ml water preservation solution, to be stored for a long time;

Immersion of green leaves and small floral series in fixative solution: Use 5% copper sulfate solution to soak the specimen until it shows a color segment of green→brown→yellow→green. The fixation time is 4 days. Remove and wash away the copper sulfate. Bleach with 2%-3% sulfurous acid for 1 day; when no pigment or impurities are precipitated, transfer to a preservation solution of 10ml of 6% sulfurous acid, 40ml of formaldehyde, 40ml of glycerin, and 1000ml of water for long-term storage;

Immersion of red flowers in fixative solution: soak in 4ml of 40% formalin, 3g of boric acid and 400ml of water for 8 days; when no pigment or impurities are precipitated, add 20ml of 10% sulfurous acid and 10g of boric acid and 1000ml of water in the preservation solution for long-term preservation;

Immersion in yellow-based fixative solution: Use 40ml of 6% sulfurous acid and 900ml of water to prepare the fixative solution, soak for 5 days; when no pigment or impurities are precipitated, transfer to 25ml of formalin, 25ml of glycerin and 1000ml of water for storage liquid, to be stored for a long time;

Crimson and purple flowers are soaked in fixative solution: soak in 20% magnesium chloride solution for 3 days, then soak in 30%, 50%, and 75% magnesium chloride solutions in sequence, each time for 6 days, and control the pH to 8; Soak in magnesium chloride solution with a concentration of 85% respectively, and after soaking for 8 days each time, keep the original color in supersaturated magnesium chloride fixative solution; when no pigment or impurities are precipitated, transfer it to the preservation solution of magnesium chloride solution with a concentration of 20% for long-term preservation .

The preparation method of the dipped specimens, the selected specimens are typical, the branches, flowers or fruits are fresh and free of disease and insect spots, the length of the branches is controlled at 10-20cm, the opening degree of the flowers is preferably 7-80%, and the fruit is sharp. Pretty and full.

According to the preparation method of the dipped specimen, the pruned branches, flowers and fruits are not damaged, cleaned and dust removed, the submerged section of the plant is 1.8-2.5 cm, shaped with a glass rod, sealed with a top cover, and stored away from light.

The preparation method of the impregnated specimen includes rinsing the impregnated specimen with clear water, fixing the shape, immersing it in the preservation solution, sealing it with paraffin, and affixing a classification label.

According to the preparation method of soaking specimens and color preservation, the soaking time for the fixative solution of soaking specimens to restore the original color of plants is 3-7 days, and that of succulent and berry plants is 60-90 days; the preservation solution can be used for a long time after one year of replacement.

In the preparation method for impregnating specimens and color retention, the containers for specimen impregnation and color fixation are all glassware or ceramic vessels.

The research experiment mechanism of the present invention shows that: the color of plants is determined by pigments, and the main components of chlorophyll, flavonoids and carotenoids that present pigments are anthocyanins. Due to the different combinations of pigment groups, the colors are also different, that is The difference in the amount of the same pigment will also lead to the difference in color.

The molecular structural formula of chlorophyll is as follows: porphyrin ring compound is composed of four pyrrole rings and four ＝CH- to form a macrocycle, the conjugated double bond forms a large π bond, and there is a magnesium atom in the center of the porphyrin ring and the ring, which determines the color of chlorophyll , so when soaking green plants, a certain concentration of copper sulfate solution is selected for substitution reaction, and the Mg ²⁺ in the porphyrin ring of the green plants is replaced by Cu ²⁺⁺ . After being replaced by Cu ²⁺ , the plants remain green. After a substitution reaction, the chlorophyll molecule has a core structure of copper atoms, and the green color presented at this time will not dissolve in organic solutions such as formaldehyde or alcohol, and has stability, thereby achieving a long-term green and fresh state.

The red parts of the plant are flowers and mature fruits, mainly carotenoids and flavonoids, flavonoids are water-soluble cytochromes, mainly anthocyanins, and the basic structure is two aromatic rings (A ring and B ring) Composition, the structural formula is shown on the right:

The number of hydroxyl groups in the B ring increases, which increases the purple color. When it contains one hydroxyl group, it appears pink or red. When it contains two hydroxyl groups, it appears purple-red. When it contains three hydroxyl groups, it appears blue-purple. The red aspect is transformed; anthocyanins are often connected with sugar molecules in the flower to form glycosides, which can make the flower color rich and bright; therefore, in the formula experiment, the concentration of formalin and magnesium chloride solution is used to fix Anthocyanin B ring hydroxyl group, and reasonably control the acid-base balance of the solution to achieve the purpose of color preservation and freshness preservation.

The production method of the present invention shows that: in the production of dipped specimens of desert plants, for the color systems of green and red two major flowers, a scientific formula is adopted, and the structure of the pigment molecule in the plant becomes Stable, to achieve the best condition of the original color of the plant body, the research mechanism and experimental design reflect the rationality and innovation of the invention concept, and highlight the progress of science and technology.

Detailed ways

The present invention will be further described below in combination with embodiments.

The preparation method of desert plant dipping specimen and color retention of the present invention is as follows:

Example 1

The dipping method of the pink and green staggered elm leaf plum specimens:

Pick elm plum branches with an opening degree of 70-80% and a length of 10-20cm, trim and arrange them so that the branches, leaves, and flowers are scattered and scattered, wash the dust on the branches, and then soak them. In order to prevent the branches from floating, tie them with heavy objects. Press, immerse the section 2-3cm; use 2ml of 40% formalin, 3g of boric acid, 10ml of 20% alcohol and 1000ml of water to fix the color, and the time is 24h; when the color is stable, no pigment or impurities are precipitated Take it out and clean it, and transfer it into a preservation solution of 30ml of 6% sulfurous acid, 20g of boric acid and 1000ml of water; seal the bottle with paraffin, and label it; it can be stored for a long time after replacing the preservation solution.

Example 2

The dipping method of the specimen of the yellow-green staggered snow lotus:

Pick snow lotus flowers with an opening degree of about 80%, the plant height is 10-20cm, and contains 5-6 yellow bracts and 4-5 green leaves; trim the branches and leaves, wash the soil at the roots, and soak them to prevent the branches from floating , tied to the root with heavy objects, immerse the section 2-3cm; put it in the refrigerator at 0-5°C, wait for the color to be fixed; use 5% copper sulfate solution to fix the color until it appears green→brown→yellow→green color section, then The process is 4-5d; remove and wash away the copper sulfate, and bleach with 2% to 3% sulfurous acid for 1d; when the color is stable and no pigment or impurities are precipitated, take it out and wash it, and immerse it in 10ml of 6% sulfurous acid, 40ml of formaldehyde, and glycerin In the 40ml and 1000ml water preservation solution, seal the bottle with paraffin and label it; it can be stored for a long time after the preservation solution is replaced.

This method can also be used to impregnate Azalea, Haloxylon and Ephedra plants.

Example 3

The dipping method of rose specimens with rose red flowers:

Pick Chinese rose branches with an opening degree of 70-80%, with a length of 10-20cm and a flower belt of 5-6 leaves; trim and arrange the branches, leaves, and flowers in a sparse manner, wash the dust on the branches, and then soak them. Float up, use heavy objects to bind and press, and immerse the section 2-3cm; use 4ml of 40% formalin, 3g of boric acid and 400ml of water to fix the color for 8 days; when the color is stable, no pigment or impurities are precipitated Take it out and clean it, immerse it in 20ml of 10% sulfurous acid, 10g of boric acid and 1000ml of water preservation solution, seal the bottle mouth with paraffin, and stick a label; it can be stored for a long time after the preservation solution is replaced.

Example 4

The dipping method of yellow marigold specimens:

Pick marigold branches with an opening degree of 80-90%, and the length of the bar is 10-20cm; the flowers have 9-10 leaves; pruning and finishing, the leaves and flowers are scattered, and the dust on the branches can be soaked in water to prevent the branches from floating , use heavy objects to bind and press, immerse the section 2-3cm; use 40ml of 6% sulfurous acid and 900ml of water to fix the color for 5 days; when the color is stable and no pigment or impurities are precipitated, take it out and clean it 25ml of formalin, 25ml of glycerin and 1000ml of water in the preservation solution, seal the bottle with paraffin, and label it; it can be stored for a long time after the preservation solution is replaced.

Example 5

Dipping method of Ageratum thistle with purple flowers:

Pick ageratum branches with an openness of 80% to 90%, length 5-10cm, 3-4 flowers with 9-10 leaves; trim the branches, leaves, and flowers to make them sparse, wash the branches with water, and then soak them , in order to overcome the floating of the branches, use heavy objects to bind the pressure, and immerse the section 2cm; soak in 20% magnesium chloride solution for 1-4d, and then soak in 30%, 50%, and 75% magnesium chloride solution in sequence, each soaking 5-7d , the pH is adjusted to 8, and then soaked in 85% magnesium chloride solution, soaking for 7 to 10 days each time, maintaining the original color in the supersaturated magnesium chloride fixative solution; Immerse in 20% magnesium chloride solution preservation solution, seal the bottle mouth with paraffin, and stick a label; it can be stored for a long time after replacing the preservation solution.

Claims (8)

1. The method for making desert plant dipping specimens and color preservation, which is characterized in that: the preparation of dipping specimens includes: material selection and collection → pruning and cleaning → fixation liquid immersion → preservation liquid immersion → bottle sealing and labeling; its color preservation and fixation The soaking steps of liquid and preservation liquid are: Immersion in pink flower fixative solution: use 1.8-4ml of 40% formalin, 2-4g boric acid, 20% alcohol 8-12ml and 800-1200ml water, soak for 22-26h; When the pigment or impurities are precipitated, transfer it to a preservation solution of 20-40ml of 6% sulfurous acid, 18-22g of boric acid and 900-1100ml of water for long-term storage; Immersion in fixative solution for green leaves and small floral series: Use 5% copper sulfate solution to soak the specimen until it turns green→brown→yellow→green. The fixation time is 3-6d, remove and wash off the sulfuric acid For copper, bleach with sulfurous acid at a concentration of 2% to 3% for 1-2 days; when no pigment or impurities are precipitated, add 8-12ml of sulfurous acid with a concentration of 6%, 38-42ml of formaldehyde, 38-42ml of glycerin, and 900-1100ml of water. In the preservation solution, to be stored for a long time; Soaking in fixed solution of red flowers: use 3-5ml concentration of 40% formalin, 2-4g boric acid and 380-420ml water, soak for 7-10d; -22ml of 10% sulfurous acid, 8-12g of boric acid and 900-1100ml of water in a preservation solution for long-term preservation; Immersion in yellow fixative solution: Use 38-42ml of 6% sulfurous acid and 800-1000ml of water to prepare the fixative solution, soak for 4-7d; when no pigment or impurities are precipitated, transfer to 23-27ml formalin , 23-27ml glycerin and 900-1100ml water preservation solution, to be stored for a long time; Immersion in fixative solution for dark red and purple flowers: Soak in 20% magnesium chloride solution for 2-5 days, then soak in 30%, 50%, and 75% magnesium chloride solutions in sequence, each time for 4-8 days, and control the pH 7.5-9; then soaked in 85% magnesium chloride solution respectively, after soaking for 6-10 days each time, keep the original color in supersaturated magnesium chloride fixative solution; when no pigment or impurities are precipitated, move into 20% magnesium chloride solution storage solution for long-term storage. 2. The preparation method of impregnating specimens and retaining color according to claim 1, characterized in that: the steps of immersing the fixative and preservation solution are as follows: Immersion in pink flower fixative solution: use 2-3ml concentration of 40% formalin, 2-3g boric acid, concentration 20% alcohol 9-11ml and 900-1100ml water mixture, soak for 23-25h; When the pigment or impurities are precipitated, transfer it to a preservation solution of 25-35ml of 6% sulfurous acid, 19-21g of boric acid and 950-1050ml of water for long-term storage; Immersion in fixative solution for green leaves and small floral series: Use 5% copper sulfate solution to soak the specimen until it turns green→brown→yellow→green. The fixation time is 3-5d, remove and wash off the sulfuric acid For copper, bleach with 2%-3% sulfurous acid for 1-1.5 days; when no pigment or impurities are precipitated, add 9-11ml of 6% sulfurous acid, 39-41ml of formaldehyde, 39-41ml of glycerin, and 950-1050ml of water in the storage solution for long-term storage; Soaking in fixed solution of red flowers: use 3-5ml of 40% formalin, 2-4g of boric acid and 380-420ml of water, soak for 7-9d; -21ml of 10% sulfurous acid, 9-11g of boric acid and 950-1050ml of water in a preservation solution for long-term preservation; Immersion in yellow fixative solution: Use 39-41ml of 6% sulfurous acid and 850-950ml of water to prepare the fixative solution, soak for 4-6 days; when no pigment or impurities are precipitated, transfer to 24-26ml formalin , 24-26ml glycerin and 950-1050ml water preservation solution, to be stored for a long time; Crimson and purple flowers are soaked in fixative solution: Soak in 20% magnesium chloride solution for 2-4 days, then soak in 30%, 50%, and 75% magnesium chloride solutions in sequence, each time for 5-7 days, and control the pH 7.8-8.5; and then soaked in 85% magnesium chloride solution respectively, after soaking for 7-9 days each time, keep the original color in supersaturated magnesium chloride fixative solution; when no pigment or impurities are precipitated, move into 20% magnesium chloride solution storage solution for long-term storage. 3. The preparation method of impregnating specimens and preserving color according to claim 1, characterized in that: the steps of immersing the fixative and preservation solution are as follows: Immersion of pink flower series fixative solution: use 2ml concentration of 40% formalin, 3g boric acid, concentration 20% alcohol 10ml and 1000ml water, soak for 24 hours; when no pigment or impurities are precipitated, transfer to 30ml concentration 6 % sulfurous acid, 20g boric acid and 1000ml water preservation solution, to be stored for a long time; Immersion of green leaves and small floral series in fixative solution: Use 5% copper sulfate solution to soak the specimen until it shows a color segment of green→brown→yellow→green. The fixation time is 4 days. Remove and wash away the copper sulfate. Bleach with 2%-3% sulfurous acid for 1 day; when no pigment or impurities are precipitated, transfer to a preservation solution of 10ml of 6% sulfurous acid, 40ml of formaldehyde, 40ml of glycerin, and 1000ml of water for long-term storage; Immersion of red flowers in fixative solution: soak in 4ml of 40% formalin, 3g of boric acid and 400ml of water for 8 days; when no pigment or impurities are precipitated, add 20ml of 10% sulfurous acid and 10g of boric acid and 1000ml of water in the preservation solution for long-term preservation; Immersion in yellow-based fixative solution: Use 40ml of 6% sulfurous acid and 900ml of water to prepare the fixative solution, soak for 5 days; when no pigment or impurities are precipitated, transfer to 25ml of formalin, 25ml of glycerin and 1000ml of water for storage liquid, to be stored for a long time; Crimson and purple flowers are soaked in fixative solution: soak in 20% magnesium chloride solution for 3 days, then soak in 30%, 50%, and 75% magnesium chloride solutions in sequence, each time for 6 days, and control the pH to 8; Soak in magnesium chloride solution with a concentration of 85% respectively, and after soaking for 8 days each time, keep the original color in supersaturated magnesium chloride fixative solution; when no pigment or impurities are precipitated, transfer it to the preservation solution of magnesium chloride solution with a concentration of 20% for long-term preservation . 4. The method for making dipped specimens according to claim 1, characterized in that: the selected specimens are typical in characteristics, the branches, flowers or fruits are fresh and free of disease and insect spots, the length of the branches is controlled at 10-20cm, and the openness of the flowers The best is 70-80%, the fruit is sharp and full. 5. The method for making dipped specimens according to claim 1, characterized in that: pruned branches, flowers, and fruits are not damaged, cleaned and dust removed, the submerged section of the plant is 1.8-2.5 cm, shaped with a glass rod, sealed with a top cover, Store away from light. 6. The method for making an impregnated specimen according to claim 1, characterized in that: rinse the impregnated specimen with clear water, fix the shape, immerse in the preservation solution, seal it with paraffin, and affix classification labels. 7. The preparation method for soaking specimens and color retention according to claims 1, 2, and 3, characterized in that: the soaking time for the fixative solution of soaking specimens to restore the original color of plants is 3-7 days, and for succulent and berry plants 60-90 days ; The preservation solution can be used for a long time after one year of replacement. 8. The preparation method for impregnating specimens and preserving color according to claims 1, 2, and 3, characterized in that the containers used for specimen impregnation and color-fixing storage are glassware or ceramic vessels.