CN101278680B - Insect baculovirus pesticides in dosage form of water dispersible granule and preparation - Google Patents

Abstract

The invention relates to a water-dispersible granule dosage form of an insect baculovirus insecticide and its preparation, which is a solid water-dispersible granule dosage form, containing insect baculovirus powder and auxiliary agents with a content of 10-500 billion PIB/g; auxiliary agents Including carrier, dispersant, wetting agent, disintegrating agent, photoprotectant, and binder; the weight ratio of each component is as follows: insect baculovirus powder 1-50; carrier 1-70; dispersant 1-20; wetting agent 0-20; disintegrant 1-20; light protection agent 0.5-20; binder 1-20. The preparation method is to mix the original virus powder and the auxiliary agent in proportion, and obtain it after jet crushing, granulation and drying; it can be used alone or in combination with chemical insecticides. Its production and processing process has low pollution, long shelf life, stable product quality, good dispersibility, easy storage, convenient transportation, ultra-low dosage, high virus content, and is of great significance to the prevention and control of agricultural and forestry pests.

Description

A kind of water dispersible granule dosage form insect baculovirus insecticide and preparation method

technical field

The invention relates to an insect baculovirus insecticide and a preparation method thereof, in particular to an ultra-low dosage and high content water-dispersible granule type insect baculovirus insecticide and a preparation method.

Background technique

The main hosts of Baculoviridae viruses are insects, which can cause the host insects to suffer from baculovirus diseases and die, and can be made into insecticides to control agricultural and forestry pests. Insect baculovirus insecticides (referred to as virus insecticides) are a class of pure natural, green and environmentally friendly biological pesticides. At present, the amount of chemical pesticides used per unit area is getting higher and higher, and the harm to the environment, non-target animals, biodiversity and humans is becoming more and more serious. A large number of highly toxic and high-residue pesticides are restricted in production, Prohibition, restriction and prohibition, the production, development and application of viral insecticides are developing rapidly, and the market demand is increasing year by year.

The production of virus insecticides consists of two parts: the production of insect virus raw powder and the processing of virus insecticide preparations. The basic principle of raw powder production is to make insects suffer from insect virus diseases by oral administration of the corresponding virus, the virus will amplify in large quantities in the insect body, and then extract the virus from the dead insect corpses to make raw powder, which is a virus-killing insecticide. active ingredients in insecticides. Preparation processing is the process of adding appropriate auxiliaries and other substances to the original powder, so that it can be directly used for field spraying and can maximize the virus and insecticidal activity.

Since insect viruses are a class of living biological products, it is especially important to protect their insecticidal activity from damage during the process of processing virus powder into preparations. For example, in an alkaline environment, the polyhedrin protein of the virus will dissolve quickly, and the activity of the virus will be destroyed; if it is stored in an aqueous solution for a long time, it is easy to produce bacteria in the product, which will affect the quality and use of the product; the ambient temperature is too high Or ultraviolet radiation, etc., will cause a sharp decline in the insecticidal activity of the virus. Therefore, it is very important for the large-scale promotion and application of this type of biological pesticide to develop a virus insecticide dosage form product suitable for storage, transportation and stable effect.

Compared with chemical insecticides, insect baculovirus insecticides have fewer types of development, and the research and development time is later, and the research basis and level are relatively low. The research and development of existing virus preparations are all based on the technology and methods of chemical insecticides. However, the physical and chemical properties and insecticidal mechanisms of insect baculoviruses have completely different characteristics. In terms of the performance of the insecticidal effect of insect viruses, it is redundant or even harmful.

At present, the dosage forms of registered insect virus insecticide products mainly include wettable powder and suspension concentrate. High, the amount of application in the field is large (generally, the amount per mu in the field is 40-100mL (grams) or more), which brings a series of problems such as inconvenient packaging, storage, transportation, sales, and virus denaturation and inactivation during the production and sales of products. . For example, in the processing of wettable powder in virus insecticides, referring to the process of conventional chemical insecticide wettable powders, a large amount (60%-90%) of inert fillers, such as diatomaceous earth, kaolin, etc., are added, which causes processing Process dust pollution, reduced virus content in preparations. Taking cotton bollworm nuclear polyhedrosis virus (Helicoverpa armigera nuclear polyhedrosis virus, referred to as HaNPV) as an example, the content of the original drug is 10-20 billion PIB/mL (grams). A certain amount of auxiliary agent, so that the polyhedron content of the cotton bollworm nuclear polyhedrosis virus preparation is between 1 billion and 2 billion PIB/mL (grams), and the dilution of the original drug concentration is about 10 times. For example, the existing wettable powder products include Henan Boai Huifeng Biochemical Pesticide Co., Ltd. registered product: 1 billion PIB/gram cotton bollworm nuclear polyhedrosis virus wettable powder, registration number: LS94579; Registered product of Engineering Co., Ltd.: 1 billion PIB/gram cotton bollworm nuclear polyhedrosis virus wettable powder, registration number: LS20011685, etc. Suspending agent products include, registered product of Huanye Biopesticide Factory in Shijiazhuang City, Hebei Province: 2 billion PIB/ml cotton bollworm nuclear polyhedrosis virus suspension concentrate, registration number: LS96795; registered product of Zhuhai Huaxia Biological Preparations Co., Ltd., Guangdong Province : 2 billion PIB/milliliter cotton bollworm nuclear polyhedrosis virus suspension, registration number: LS20031797, etc.

Wettable powders and suspensions have their own advantages and disadvantages in transportation and application: for example, suspensions are not convenient for storage and transportation, and wettable powders are superior to suspensions in maintaining the biological activity of viruses, but because the preparations are diluted with water when applied in the field In some cases, limited by the technical level of processing, the dispersibility of the virus polyhedron is not as good as that of the suspension, resulting in a lower field application effect than the suspension.

Contents of the invention

The invention provides a water-dispersible granule formulation insect rod with insect baculovirus as the main active ingredient, which overcomes the shortcomings of suspension concentrate and wettable powder, has good dispersibility, is easy to store, and is convenient to transport, and has ultra-low dosage and high content. Symptovirus insecticide and preparation method.

Technical scheme of the present invention is as follows:

The water-dispersible granule formulation insect baculovirus insecticide provided by the present invention is characterized in that the water-dispersible granule formulation insect baculovirus insecticide is a solid water-dispersible granule formulation, comprising insect baculovirus powder and Auxiliary agent; the insect baculovirus content of the insect baculovirus powder is 100-500 billion PIB/gram; the auxiliary agent includes a carrier, a dispersant, a wetting agent, a disintegrant, a photoprotectant, and an adhesive Binder;

The proportioning by weight of each component is as follows:

1-50 parts by weight of insect baculovirus powder;

Carrier 1-70 parts by weight;

Dispersant 1-20 parts by weight;

0-20 parts by weight of wetting agent;

1-20 parts by weight of disintegrant;

0.5-20 parts by weight of photoprotectant;

1-20 parts by weight of binder.

The insect baculovirus powder is a nuclear polyhedrosis virus powder or/and a granulosis virus powder.

The carrier is white carbon black, bentonite, kaolin, diatomaceous earth or calcium carbonate;

Described dispersant is a kind of surface active substance, can be anionic surfactant, cationic surfactant, amphoteric surfactant and nonionic surfactant; More preferably sodium lauryl laurate, lauryl Sodium benzenesulfonate, sodium lauryl sulfate, sodium lignin, calcium lignin or tea saponin, etc.;

Described wetting agent is a kind of surface active substance, can be anionic surfactant, cationic surfactant, amphoteric surfactant and nonionic surfactant; More preferably sodium lauryl laurate, lauryl Sodium phenyl sulfonate, sodium lauryl sulfate, sodium lignin, calcium lignin or tea saponin, etc.;

The disintegrating agent is croscarmellose sodium, croscarmellose sodium starch, ammonium sulfate, sodium bicarbonate, starch or urea, etc.;

The photoprotectant is fluorescent whitening agent, India ink, nano-titanium dioxide, nano-zinc oxide or activated carbon powder, etc.;

The binder is soluble starch, gelatin, polyvinyl alcohol, carboxymethyl cellulose or sodium carboxymethyl cellulose and the like.

The preparation method of water-dispersible granule dosage form insect baculovirus insecticide provided by the invention, its steps are as follows:

1) Mix the original powder of insect baculovirus, carrier, dispersant, wetting agent, disintegrant, photoprotectant and binder according to the following proportions by weight and perform pre-crushing;

2) The pre-pulverized raw material is then crushed by air flow to make the particle size less than 10 μm;

3) In the mixer, the raw materials after airflow pulverization are mixed evenly again;

4) Add water for kneading, extrude and granulate with a granulator; or granulate with a fluidized bed;

5) Vacuum-drying or freeze-drying it afterwards, the drying temperature is not higher than 40°C, to obtain water-dispersed particles with a moisture content of not more than 5%;

6) Sieving the obtained water-dispersible granules to obtain a water-dispersible granule dosage form insect baculovirus insecticide.

The water-dispersible granule formulation type insect baculovirus insecticide of the present invention can be used alone or in combination with chemical insecticides to prevent and control agricultural and forestry pests.

Compared with insect baculovirus suspensions and wettable powders, the water-dispersible granule formulation insect baculovirus insecticide of the present invention has many irreplaceable advantages: (1) the dust pollution in the processing and field application process is very small, and the The operator is safe; (2) the moisture content is very small (less than 5%), the biological activity of the virus is maintained for a long time, the shelf life is long, and the product quality is stable; (3) the ambient temperature during the processing is not higher than 40°C, and the The insecticidal activity of the virus is maintained; (4) the virus content is high (between 100-500 billion PIB/g), the insecticidal effect is good, the amount used in the field is small, and it is easy to transport; (5) the fluidity is good, the container is less attached, After adding water, it has good disintegration and is easy to use; (6) due to the high content of active ingredients, the dispersant and light protection agent in the auxiliary agent can fully play their roles, so that the virus polyhedron is well dispersed in the field and has strong UV resistance; (7) The auxiliaries used are all water-based substances, and the amount used in the field is very small (only 0.2-10 grams per mu), which is very friendly to the environment and is a green and environmentally friendly pesticide product.

Detailed ways

Example 1

500 billion PIB/gram of cotton bollworm nuclear polyhedrosis virus powder 2Kg, add sodium dodecylbenzene sulfonate (also can be sodium dodecyl laurate) 10Kg, lignin calcium 10Kg, urea 5Kg, fluorescence increase Whitening agent (also can be nano zinc oxide) 2Kg, kaolin 66Kg, carboxymethyl cellulose 5Kg. The pulverizer mixes and pulverizes to become mother powder. The mother powder is further pulverized with a jet mill to make the particle size less than 10 μm, and then fully mixed in a mixer. After being granulated by a fluidized bed, it is vacuum-dried, and the drying temperature is controlled at 30°C. When the moisture content is lower than 5%, the material is taken and sieved, and the finished product rate is 95%. Promptly obtain 95Kg of 10,000,000,000 PIB/gram of cotton bollworm nuclear polyhedrosis virus water-dispersible granules product, and need 100,000,000,000 PIBs of cotton bollworm virus to prevent and control cotton bollworm according to every mu of cotton field, then its field consumption is 10 grams/mu.

For field control, use 10 grams of 10 billion PIB/gram of water-dispersible granules per mu of cotton field and spray with 40Kg of water. After 7 days, the control effect on cotton bollworm can reach more than 80%, and it is safe to natural enemies.

Example 2

200 billion PIB/gram of Spodoptera litura nuclear polyhedrosis virus (Spodoptera litura nuclear polyhedrosis virus) powder 15Kg, add sodium lauryl sulfate 15Kg, tea saponin (also can be lignin calcium) 12Kg, ammonium sulfate (also Can be sodium bicarbonate) 15Kg, nano titanium dioxide 5Kg, bentonite 32Kg, soluble starch 6Kg. The pulverizer mixes and pulverizes to become mother powder. The mother powder is further pulverized with a jet mill to make the particle size less than 10 μm, and then fully mixed in a mixer. After extrusion granulation, vacuum drying, the drying temperature is controlled at 30°C. When the moisture content is lower than 5%, the material is taken and sieved, and the finished product rate is 95%. Promptly obtain 95Kg of 30,000,000,000 PIB/gram Spodoptera litura nucleopolyhedrosis virus water dispersible granules product, calculate by the Spodoptera litura virus control Spodoptera litura virus that needs 60,000,000,000 PIBs per mu of vegetable field, then its field consumption is 2 grams/ mu.

During field control, use 2 grams of 30 billion PIB/gram of water-dispersible granules per mu of vegetable field, and spray with 30Kg of water. After 7 days, the control effect on Spodoptera litura can reach more than 80%, and it is safe to natural enemies.

Example 3

600 billion PIB/gram of Plutella xylostella granulosis virus (Plutella xylostella granulosis virus) former powder 10Kg, add tea saponin 15Kg, starch 5Kg, optical brightener 15Kg, bentonite 49Kg, polyvinyl alcohol 6Kg. The pulverizer mixes and pulverizes to become mother powder. The mother powder is further pulverized with a jet mill to make the particle size less than 10 μm, and then fully mixed in a mixer. After extrusion granulation, freeze-drying in vacuum. After the drying is finished, the material is taken and sieved, and the finished product rate is 90%. Promptly obtain 60,000,000,000 PIB/gram Plutella xylostella granule virus water dispersible granule product 90Kg, by the Plutella xylostella virus control diamondback moth that needs 600,000,000,000 PIB per acre of vegetable field, then its field consumption is 10 grams/mu.

During field control, use 10 grams of water-dispersible granules of 60 billion PIB/gram per mu of vegetable field, and spray with 30Kg of water. After 5 days, the control effect on diamondback moth can reach more than 85%, and it is safe to natural enemies.

Example 4

1Kg of the original powder of Pieris rapae granulosis virus (Pieris rapae granulosis virus) of 1000 billion PIB/gram, add 1Kg of sodium lauryl sulfate, 20Kg of calcium lignin, 20Kg of cross-linked sodium carboxymethyl starch, 20Kg of activated carbon powder, carbonic acid Calcium 37Kg, gelatin 1Kg. The pulverizer mixes and pulverizes to become mother powder. The mother powder is further pulverized with a jet mill to make the particle size less than 10 μm, and then fully mixed in a mixer. After extrusion granulation, freeze-drying in vacuum. After the drying is finished, the material is taken and sieved, and the finished product rate is 98%. Promptly obtain 98Kg of 10,000,000,000 PIB/gram of rapae worm granule virus water dispersible granule product, need 100,000,000,000 PIB of rapae worm virus prevention and control cabbage by every mu vegetable field, then its field consumption is 10 grams/mu.

During field control, use 10 grams of 10 billion PIB/gram of water-dispersible granules per mu of vegetable field and spray with 30Kg of water. After 6 days, the control effect on cabbage caterpillar can reach more than 87%, and it is safe to natural enemies.

Example 5

300 billion PIB/gram of Spodoptera exigua nuclear polyhedrosis virus (Spodoptera exigua nuclear polyhedrosis virus) powder 25Kg, add tea saponin 10Kg, sodium dodecylbenzene sulfonate 10Kg, croscarmellose sodium 12Kg, India ink 0.5Kg, calcium carbonate 32.5Kg, polyvinyl alcohol 10Kg. The pulverizer mixes and pulverizes to become mother powder. The mother powder is further pulverized with a jet mill to make the particle size less than 10 μm, and then fully mixed in a mixer. After extrusion granulation, freeze-drying in vacuum. After the drying is finished, the material is taken and sieved, and the finished product rate is 95%. Promptly obtain 95Kg of 75,000,000,000 PIB/gram beet armyworm nuclear polyhedrosis virus water-dispersible granule product, and the beet armyworm virus control beet armyworm that needs 75,000,000,000 PIBs per mu of vegetable field calculates, then its field consumption is 1 gram/ mu.

For field control, use 1 gram of 75 billion PIB/gram of water-dispersible granules per mu of vegetable field, and spray with 30Kg of water. After 5 days, the control effect on beet armyworm can reach more than 90%, and it is safe to natural enemies.

Example 6

100 billion PIB/gram of Ectropis obliqua nuclear polyhedrosis virus (Ectropis obliqua nuclear polyhedrosis virus) powder 50Kg, add tea saponin 15Kg, urea 5Kg, fluorescent whitening agent 20Kg, diatomaceous earth 9Kg, soluble starch 1Kg. The pulverizer mixes and pulverizes to become mother powder. The mother powder is further pulverized with a jet mill to make the particle size less than 10 μm, and then fully mixed in a mixer. After extrusion granulation, freeze-drying in vacuum. After the drying is finished, the material is taken out and sieved, and the finished product rate is 99%. Promptly obtain 99Kg of 50 billion PIB/gram tea geometrid nuclear polyhedrosis virus water dispersible granules product, and the tea geometrid virus prevention and control tea geometrid meter that needs 100 billion PIB per mu of tea garden, then its field consumption is 2 grams/mu.

When preventing and controlling, use 2 grams of water-dispersible granules of 100 billion PIB/gram per mu of tea garden, and spray with 40Kg of water. After 9 days, the control effect on tea geometrids can reach more than 90%, and it is safe to natural enemies.

Example 7

1000 billion PIB/gram of Agrotis segetum granulosis virus (Agrotis segetum granulosis virus) former powder 50Kg, add lignin calcium 10Kg, sodium lauryl laurate 2Kg, starch 3Kg, nano titanium dioxide 10Kg, white carbon black 5Kg, carboxylate Methyl cellulose 20Kg. The pulverizer mixes and pulverizes to become mother powder. The mother powder is further pulverized with a jet mill to make the particle size less than 10 μm, and then fully mixed in a mixer. After extrusion granulation, freeze-drying in vacuum. After the drying is finished, the material is taken and sieved, and the finished product rate is 94%. Promptly obtain 94Kg of 500,000,000,000 PIB/gram of yellow cutworm granule virus water dispersible granule product, need 750,000,000,000 PIBs of yellow cutworm virus control yellow cutworm according to every mu of farmland, then its field consumption is 1.5 grams/mu.

When preventing and controlling, use 1.5 grams of water-dispersible granules of 1 trillion PIB/gram per mu of farmland, and spray with 30Kg of water. After 7 days, the control effect on the yellow cutworm can reach more than 90%, and it is safe to natural enemies.

Example 8

300 billion PIB/gram of beet armyworm nuclear polyhedrosis virus powder 10Kg, 600 billion PIB/gram of Plutella xylostella granulosa virus powder 40Kg, add tea saponin 2Kg, lignin calcium 2Kg, cross-linked carboxymethyl fiber Su Na 10Kg, nano zinc oxide 1Kg, diatomaceous earth 25Kg, polyvinyl alcohol 10Kg. The pulverizer mixes and pulverizes to become mother powder. The mother powder is further pulverized with a jet mill to make the particle size less than 10 μm, and then fully mixed in a mixer. After extrusion granulation, freeze-drying in vacuum. After the drying is finished, the material is taken and sieved, and the finished product rate is 96%. That is, 96Kg of water dispersible granule product mixed with 30 billion PIB/gram of beet armyworm nuclear polyhedrosis virus and 240 billion PIB/gram of diamondback moth granule virus is obtained, and 75 billion PIB of beet armyworm virus control is required per mu of vegetable field Beet armyworm, 600 billion PIB of Plutella xylostella granulosa virus control Plutella xylostella, in vegetable fields where the two pests are mixed, the field dosage is 5 grams/mu.

In vegetable fields where co-occurrence of Spodoptera and Plutella xylostella, a water dispersible granule product with a mixture of two viruses (30 billion PIB/g Spodoptera nucleopolyhedrosis virus and 240 billion PIB/g Plutella xylostella granulovirus) 5 grams, mixed with 30Kg of water and sprayed, after 5 days, the control effects on beet armyworm and diamondback moth respectively reach more than 90%, and it is safe to natural enemies.

Claims (4)

1. A water-dispersible granule formulation insect baculovirus insecticide, characterized in that, said water-dispersible granule formulation insect baculovirus insecticide is a solid water-dispersible granule formulation, comprising insect baculovirus former powder and auxiliary agent; the insect baculovirus content of the insect baculovirus powder is 100-500 billion PIB/gram; the auxiliary agent includes carrier, dispersant, wetting agent, disintegrant, photoprotectant and adhesive Binder; The proportioning by weight of each component is as follows: 1-50 parts by weight of insect baculovirus powder, the particle size of which is less than 10 μm; 1-70 parts by weight of carrier, which is bentonite or calcium carbonate; 1-20 parts by weight of a dispersant, which is sodium dodecyl laurate, sodium dodecylbenzenesulfonate, sodium dodecyl sulfate or tea saponin; 0-20 parts by weight of wetting agent, which is sodium lauryl laurate, sodium dodecylbenzenesulfonate, sodium lauryl sulfate, sodium lignin, calcium lignin or tea saponin; 1-20 parts by weight of disintegrant, which is cross-linked sodium carboxymethylcellulose or cross-linked sodium carboxymethyl starch; 0.5-20 parts by weight of photoprotectant; 1-20 parts by weight of binder, which is soluble starch, gelatin or sodium carboxymethyl cellulose; The insect baculovirus powder is nuclear polyhedrosis virus or/and granulovirus. 2. water dispersible granule formulation insect baculovirus insecticide according to claim 1, is characterized in that, described photoprotective agent is fluorescent whitening agent, Indian ink, nano-titanium dioxide, nano-zinc oxide or active carbon pink. 3. a preparation method of water-dispersible granule formulation insect baculovirus insecticide according to claim 1, its steps are as follows: 1) Mix the original powder of insect baculovirus, carrier, dispersant, wetting agent, disintegrant, photoprotectant and binder according to the following proportions by weight and perform pre-crushing; The proportioning by weight of each component is as follows: 1-50 parts by weight of insect baculovirus powder; 1-70 parts by weight of carrier, which is bentonite or calcium carbonate; 1-20 parts by weight of dispersant, which is sodium lauryl laurate, sodium dodecylbenzenesulfonate, sodium lauryl sulfate or tea saponin; 0-20 parts by weight of wetting agent, which is sodium lauryl laurate, sodium dodecylbenzenesulfonate, sodium lauryl sulfate, sodium lignin, calcium lignin or tea saponin; 1-20 parts by weight of disintegrant, which is cross-linked sodium carboxymethylcellulose or cross-linked sodium carboxymethyl starch; 0.5-20 parts by weight of photoprotectant; 1-20 parts by weight of binder, which is soluble starch, gelatin or sodium carboxymethyl cellulose; The original powder of insect baculovirus is nuclear polyhedrosis virus or/and granular virus; 2) The pre-pulverized raw material is then crushed by air flow to make the particle size less than 10 μm; 3) In the mixer, the raw materials after airflow pulverization are mixed evenly again; 4) Add water for kneading, extrude and granulate with a granulator; or granulate with a fluidized bed; 5) Vacuum-drying or freeze-drying it afterwards, the drying temperature is not higher than 40°C, to obtain water-dispersed particles with a moisture content of not more than 5%; 6) Sieving the obtained water-dispersible granules to obtain a water-dispersible granule dosage form insect baculovirus insecticide. 4. by the preparation method of water-dispersible granule formulation insect baculovirus insecticide according to claim 1, it is characterized in that, described photoprotective agent is fluorescent whitening agent, Indian ink, nano-titanium dioxide, nano-zinc oxide or activated carbon powder. the