CN101221182A - Novel method for blood serum tumor series diagnosis by fluorescent protein chip - Google Patents
Novel method for blood serum tumor series diagnosis by fluorescent protein chip Download PDFInfo
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- CN101221182A CN101221182A CNA2007100000552A CN200710000055A CN101221182A CN 101221182 A CN101221182 A CN 101221182A CN A2007100000552 A CNA2007100000552 A CN A2007100000552A CN 200710000055 A CN200710000055 A CN 200710000055A CN 101221182 A CN101221182 A CN 101221182A
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- fluorescent protein
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- serum tumor
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- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 31
- 210000002966 serum Anatomy 0.000 title claims abstract description 22
- 238000003745 diagnosis Methods 0.000 title claims abstract description 20
- 102000034287 fluorescent proteins Human genes 0.000 title claims abstract description 18
- 108091006047 fluorescent proteins Proteins 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000005516 engineering process Methods 0.000 claims abstract description 9
- 238000004458 analytical method Methods 0.000 claims abstract description 5
- 238000012360 testing method Methods 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 4
- 238000011160 research Methods 0.000 claims description 3
- 241000202934 Mycoplasma pneumoniae Species 0.000 claims description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 2
- 239000010931 gold Substances 0.000 claims description 2
- 229910052737 gold Inorganic materials 0.000 claims description 2
- 208000002672 hepatitis B Diseases 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 238000002331 protein detection Methods 0.000 claims description 2
- 208000006379 syphilis Diseases 0.000 claims description 2
- 210000002700 urine Anatomy 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 230000010354 integration Effects 0.000 claims 1
- 201000011510 cancer Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to a new method for fluorescent protein chip serum tumor series diagnosis, which is a fluorescent protein chip serum tumor series diagnosis new technology platform system established by collecting the serum of clinical patients with tumors, selecting tumor series antibodies to produce the chip and using a specific software for the analysis and processing of protein expression. The new technology and the new therapy can provide technical supports for reducing the incidence rate and the mortality of malignant tumor diseases and improving healing efficacy and the quality of life.
Description
Technical field:
This patent relates to field of biomedicine technology, is specifically related to a kind of novel method for blood serum tumor series diagnosis by fluorescent protein chip.
Background technology
Protein-chip is a biomedical main direction of studying of 21 century.At present, external medical institutions and drugmaker adopt protein-biochips to carry out medical diagnosis on disease and new drug development in a large number, but domestic seldom application.Also do not use protein-chip clinically.
At present, the protein-biochips system uses the laser chip machine both at home and abroad mostly, costs an arm and a leg, and operates miscellaneous.Our fluorescent protein chip be a kind of with the many analyzing and testing steps in the life science and device by being integrated in an analyzing and testing system on the chip that has only several mm square after parallelization and the miniaturized.Have with low cost, simple operation and other advantages, belong to the developing direction of biochip, have better market prospect.
Malignant tumour is second largest " killer " who is only second to angiocardiopathy in the world.Annual newly-increased tumour patient 2,000,000 people of China, dead more than 130 ten thousand people, present national cancer patient Estimate of Total Number is more than 5,000,000.This technology makes the present cost 1 week blood serum tumor series molecular diagnosis that needs shorten to several hours, and provides foundation for treatment.Market survey shows, the clinical protein-chip that is sought after.
Main innovate point:
1. this patent has been set up the blood serum tumor series diagnosis by fluorescent protein chip system.Present microslide reactive system be can replace, operation and low price are convenient to.
2. this patent has been set up the blood serum tumor series diagnosis by fluorescent protein chip new technology, has hypersensitivity and high stability, is suitable for great amount of samples and detects.
3. this patent has been set up fluorescent protein chip blood serum tumor series digit detection system, makes that the result more standardizes, accuracy.
4. utilize this technology platform also to can be used for the detection of indexs such as relevant hepatitis B e antibody, core antibody and surface antibody, syphilis, mycoplasma pneumoniae, Cox Ab and urine protein detection etc., the high flux screening that can also be used for medicine, application prospect are very wide;
Summary of the invention:
Blood serum tumor series fluorescent protein chip diagnostic techniques is the aetiology of tumour, and early molecule diagnosis, early intervention and the research of preventative strategies can in advance provide gordian technique.By gathering serum from the clinical tumor patient, select tumour series antibody for use: breast cancer antigen (CA15-3), gastrointestinal cancer antigen (CA19-9), ovarian cancer antigen (CA125), cytokeratin 19 (CYFRA21-1), sugar antigen 72-4 (CA72-4), sugar antigen-50 (CA-50), phosphorus cancer associated antigen (SCC), carcinomebryonic antigen (CEA), prostate specific antigen (PSA), free prostate gland specificity antigen (E-PSA), and alpha-fetoprotein (AFP) (0 ~ 300ng/ml), alpha-fetoprotein (AFP) (0 ~ 1000ng/ml), the short sex hormone of free B human chorionic, ferritin, B2 microglobulin, neuronspecific enolase (NSE), make chip, carry out fluorescence reaction and quantitative test.At this common disease of malignant tumour, frequently-occurring disease, obtain new technology, the new treatment of a collection of early prevention and intervention, for M ﹠ M, raising curative effect and the life quality that lowers major disease provides technical support.
Technical scheme:
1. collection of specimens: sample is from clinical tumor patient and normal person's serum.
2. making chip: select tumour series antibody for use: breast cancer antigen (CA15-3), gastrointestinal cancer antigen (CA19-9), cytokeratin 19 (CYFRA21-1), sugar antigen 72-4 (CA72-4), sugar antigen-50 (CA-50), phosphorus cancer associated antigen (SCC), ovarian cancer antigen (CA125), carcinomebryonic antigen (CEA), prostate specific antigen (PSA), free prostate gland specificity antigen (E-PSA), and alpha-fetoprotein (AFP) (0 ~ 300ng/ml), alpha-fetoprotein (AFP) (0 ~ 1000ng/ml), the short sex hormone of free B human chorionic, ferritin, B2 microglobulin, neuronspecific enolase (NSE).
3. set up the fluorescence reaction system. comprise fluorescence labeling albumen → Blocking Array-tube sealing chip pipe → Hybridization hybridization etc.
4. the protein expression chip analysis is handled with its specific software.
Prospect:
Protein-biochips is a biomedical main direction of studying of 21 century.The market sales revenue of protein-biochips is estimated to grow to more than 500,000,000 dollars in 2008 from 100,000,000 2,200 ten thousand dollars of 2002.
The method of fluorescent protein chip blood serum tumor series series diagnosis can provide a cover brand-new, complete and effective platform for modern diagnosis and new drug development.The present invention can also become the more advanced little test tube chip of nm of gold plastics by infiltration and development, can enter the international market for the diagnostic techniques industry of China a solid reliable launching platform is provided, centering state pharmaceutical sector will stand on the invincible position in future in the competition profound significance.
Claims (7)
1. latest scientific research that novel method for blood serum tumor series diagnosis by fluorescent protein chip is a field of biomedicine technology.
2. the described novel method for blood serum tumor series diagnosis by fluorescent protein chip of claim 1 is that the many analyzing and testing steps in the life science and device are integrated in an analyzing and testing system on the chip that has only several mm square.
3. the described novel method for blood serum tumor series diagnosis by fluorescent protein chip of claim 1, its used method susceptibility, stability are high, are suitable for great amount of samples and detect.
4. the described novel method for blood serum tumor series diagnosis by fluorescent protein chip of claim 1, the blood serum tumor series digit detection system of foundation is a kind of real-time continuous register system, more standard is more accurate to make the result.
5. the described novel method for blood serum tumor series diagnosis by fluorescent protein chip of claim 1 has been strengthened the system integration of biotechnology and clinical resource.Biological analysis process analysis procedure analysis speed is improved, and required sample and reagent reduce, and for treatment provides reliable basis, are the aetiology of tumour, and early molecule diagnosis, early intervention and the research of preventative strategies can in advance provide gordian technique.
6. the described novel method for blood serum tumor series diagnosis by fluorescent protein chip of claim 1 can be used for the detection of indexs such as relevant hepatitis B e antibody, core antibody and surface antibody, syphilis, mycoplasma pneumoniae, Cox Ab and urine protein detection etc.
7. the described novel method for blood serum tumor series diagnosis by fluorescent protein chip of claim 1 can develop into the little test tube chip of higher levels of nm of gold plastics through the science extension.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007100000552A CN101221182A (en) | 2007-01-08 | 2007-01-08 | Novel method for blood serum tumor series diagnosis by fluorescent protein chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100000552A CN101221182A (en) | 2007-01-08 | 2007-01-08 | Novel method for blood serum tumor series diagnosis by fluorescent protein chip |
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| CN101221182A true CN101221182A (en) | 2008-07-16 |
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| CNA2007100000552A Pending CN101221182A (en) | 2007-01-08 | 2007-01-08 | Novel method for blood serum tumor series diagnosis by fluorescent protein chip |
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