CN100594920C - Bushen Zengjing Granules - Google Patents
Bushen Zengjing Granules Download PDFInfo
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- CN100594920C CN100594920C CN200610050874A CN200610050874A CN100594920C CN 100594920 C CN100594920 C CN 100594920C CN 200610050874 A CN200610050874 A CN 200610050874A CN 200610050874 A CN200610050874 A CN 200610050874A CN 100594920 C CN100594920 C CN 100594920C
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Abstract
本发明涉及一种补肾增精颗粒剂，特别适用于治疗因肾阳衰虚所致精子减少，活力低下，死精过多的男性不育症。由肉苁蓉、淫羊霍、菟丝子、枸杞子、海龙、补骨脂、制何首乌、黄芪、银杏、蜈蚣10味中药组成。具有补肾助阳、益气生精之功效，本发明经药理学研究表明补肾增精颗粒剂对精子发生、活力及其降低畸形精子率具有明显的增强作用，以小鼠为动物模型，验证了该制剂的药理作用，并经临床预试验证实具有明显疗效，因而具有较大的推广价值。The invention relates to a granule for invigorating the kidney and increasing essence, which is particularly suitable for treating male infertility caused by decreased sperm, low vitality and excessive dead sperm due to deficiency of kidney yang. It is composed of 10 traditional Chinese medicines, including Cistanche, Epimedium, Cuscuta, Lycium barbarum, Sea Dragon, Psoralen, Radix Polygoni Multiflori, Astragalus, Ginkgo, and Centipede. It has the effects of invigorating the kidney and supporting yang, benefiting qi and generating essence. The pharmacological studies of the present invention have shown that the kidney-tonifying Zengjing granule has a significant enhancement effect on spermatogenesis, vitality and the reduction of abnormal sperm rate. Using mice as animal models, it has been verified The pharmacological action of the preparation has been proved to have obvious curative effect by pre-clinical test, so it has great popularization value.
Description
Technical field
The present invention relates to a kind of kidney-replenishing sperm-supplementing granule, especially for the treatment kidney yang oligospermia due to the void that declines, vigor is low, the male infertility that necrospermia is too much.
Background technology
Current environment pollutes and increases the weight of day by day, and human fertility is on a declining curve.61 pieces of document results that publish according to 21 countries of the Denmark scholar E.Carlsen and the A.Giwercman analysis-by-synthesis 1938-1990 whole world show that reduced more than 40% in the period of human sperm's number past 50, semen volume reduces by 20%.China is from the seventies of sperm quantity decline so far 30%.Environmental pollution causes that to the influence of healthy reproduction international community extensively payes attention to.Hold first " environment and healthy reproduction " international conference in Denmark as far back as the United Nations in 1991; Held " environment and development " summit meeting in 1992 in Brazil.
China's rural area chemical fertilizer, pesticide, herbicide, plastic sheeting for farm use etc. are a large amount of at present uses, and electronic waste spreads everywhere, has caused severe contamination.Many river courses blackout, the riverbank is weedy, and rubbish is in heaps, and the agricultural land soil harmful element exceeds standard, the sclerosis of hardening, it is healthy not only to jeopardize the peasant, and directly has influence on the safety of agricultural product, enters huge numbers of families by food chain, this gives birth to quality to the mankind and causes and have a strong impact on, now, the healthy reproduction downgrade, birth defect is high.China has more than 1,200 ten thousand couples of sterility and infertility Mr. and Mrs medical services of seeking help approximately, has every year more than 30 ten thousand birth defect babies to be born approximately, to society and family composition pressure, and present, does not have preferably medicine can treat this problem as yet to male infertility.
Chinese patent CN1088809A discloses a kind of " compound Chinese patent medicine of treatment male sterility ", though effective example report in the literary composition, but poor effect, total effective rate is not quite clear, moreover, because adopt rare Chinese medicines such as Radix Ginseng, Colla Plastri Testudinis, Colla cornus cervi in the prescription, the messenger drug high price is expensive, has influenced to apply.
Summary of the invention
The purpose of this invention is to provide a kind of can treatment because of the kidney yang oligospermia due to the void that declines, vigor is low, the medicine of the male infertility that necrospermia is too much, and the medicine valency is cheap, effect is better, with the misery that solves sterile male and the contradiction of family, make the study and work that helps the patient and build harmonious well-off society.
Kidney-replenishing sperm-supplementing granule of the present invention is made up of Herba Cistanches, Herba Epimedii, Semen Cuscutae, Fructus Lycii, the Radix Astragali, Solenognathus, Fructus Psoraleae, Radix Polygoni Multiflori Preparata, Semen Ginkgo, Scolopendra 10 flavor Chinese medicines, and its prescription is:
Herba Cistanches 160～170g, Herba Epimedii 230～270g, Semen Cuscutae 160～170g, Fructus Lycii 160～170g, the Radix Astragali 230～270g, Solenognathus 58～70g, Fructus Psoraleae 160～170g, Radix Polygoni Multiflori Preparata 230～270g, Semen Ginkgo 160～170g, Scolopendra 13～22g.
Through pharmacology, the test of pesticide effectiveness, acute toxicity test, has ideal effect.
In order to study of the effect of this medicine to spermatogenesis, vigor and reduction abnormal sperm rate thereof, this test system adopt the immaturity mice and for want of the adult mice that increases of the rate of teratosperm that causes of vitamin A be animal model, the pharmacological action of checking said preparation.
One, kidney-replenishing sperm-supplementing granule is to the influence of generation of immaturity mouse sperm and vigor thereof
(1) test material
1, animal: mice (ICR), 57, body weight (BW) 18 ± 1g is provided by Zhejiang Province's Experimental Animal Center, belongs to the conventional animal level, holds effective quality certification (No. 96001, Zhejiang doctor's laboratory animal, as follows).
2, medicine: 10 flavor Chinese medicines, preparation--extractum, wherein Solenognathus and Scolopendra are ground into fine powder, and be mixed in the prescription ratio, standby, and all the other 8 flavor Chinese medicines add 10 times in water, decoct 3 times.The 1st 2h, the 2nd 1.5h, the 3rd 1h, merging filtrate is concentrated in right amount promptly.Final extractum concentration is 2.08g crude drug/ml.Lot number is 98116.
3, instrument: phase contrast microscope; Water bath with thermostatic control; The rigorous analysis balance; Acidometer etc.
(2) EXPERIMENTAL DESIGN and method thereof
1, blank group, 6 of male mices are only obeyed water, do not obey other any medicines.
2, kidney-replenishing sperm-supplementing granule group: 45 of male mices.(1.4g crude drug/kgBW～11.2g crude drug/kgBW) is divided into 4 groups of (see figure 1)s by various dose; The 6th group of kidney-replenishing sperm-supplementing granule group (be called for short and lack 2 flavor groups) for lacking Solenognathus and Scolopendra, 8; The 7th group of kidney-replenishing sperm-supplementing granule list 2 flavor groups, i.e. Solenognathus and Scolopendra 2 flavor groups, 9.
3, the kidney-replenishing sperm-supplementing granule group is as positive controls, 6.
Above-mentioned each group (except that blank), administration every day 1 time, continuous 1 month.Behind the last administration 24h, put to death animal, take out cauda epididymis tissue, put in embryo's operation glass, add immediately in 1: 40 mouse sperm culture fluid with the cervical vertebra dislocation method; Cut fully with ophthalmologic operation and to shred, and put and hatch 10min in 37 ℃ of constant water bath box and make sperm fully be released in the culture fluid; Reuse Macracloth filters rapidly, removes connective tissue etc.; Use precision liquid taking device then, get 10 μ l on clean glass slide, add coverslip (22 * 22mm), put (* 40) under the phase contrast microscope, by The World Health Organization's 1992 standards, observe sperm motility rate (the sperm survival rate expects that with 2% blue repulsion test confirms), count 200 sperms at least; And use blood counting chamber according to a conventional method, calculate sperm concentration.
(3) result
1, sperm motility rate: sperm motility rate increases with medication dose, but each group and matched group and QIANGJING KELI agent group are relatively, not statistically significant (Fig. 1).
2, motility of sperm: (1.4～5.6g crude drug/kgBW) and obviously increase, wherein 2.8g crude drug and 5.6g crude drug/kg group compares P＜0.05 with matched group with medication dose.Though and the kidney-replenishing sperm-supplementing granule motility of sperm increases to some extent than matched group, not statistically significant (P＞0.05, Fig. 2-3).
3, sperm concentration: (1.4g～5.6g crude drug/kg) obviously increase with medication dose compares P＜0.05～P＜0.01 with matched group to the kidney-replenishing sperm-supplementing granule group.Under same dose (10 times of plan clinical dosage groups), the sperm concentration of QIANGJING KELI agent group is greater than the kidney-replenishing sperm-supplementing granule group.See Fig. 4.
Two, kidney-replenishing sperm-supplementing granule is to the influence of adult mice motility of sperm due to the vitamin A for want of and abnormal rate thereof
Get 20 of adult mices, 8 monthly ages, body weight 45 ± 3g.From 8 monthly ages, change and feed the A feedstuff that is deficient in vitamin, finish until experiment, and implement following test.
(1) to the influence of mice external sperm vigor
The same test of sperm processing method is adjusted to 10 with sperm count
6Cells/ml; Be divided into 4 groups of (see figure 5)s, every group of volume is that 0.25ml carries out the influence experiment of kidney-replenishing sperm-supplementing granule to mice external sperm vigor:
1, matched group: do not add any medicine;
2, kidney-replenishing sperm-supplementing granule 2.2g/ml group (being equivalent to intend) with 5 times of clinical dosages;
3, kidney-replenishing sperm-supplementing granule 4.4g group (plan) with 10 times of clinical dosages;
4,8.8g (plan) group with 20 times of clinical dosages;
Put 37 ℃ of water-soluble casees of constant temperature then, continuous culture 4h.Respectively 0,1,2,3 and observe sperm " a+b " level vigor during 4h.
(see figure 5) as a result: matched group motility of sperm (a+b) reduces gradually with the prolongation of incubation time, drops to 20% from 45%; And administration group motility of sperm (a+b) descends slowly though with the prolongation of incubation time decline is arranged.When 3h, the 4.4g group still maintains 43%, and matched group only is 24%.8.8g the group motility of sperm can reach 50%, shows that kidney-replenishing sperm-supplementing granule has tangible potentiation to motility of sperm.
(2) to the influence of the A induced mice rate of teratosperm that is deficient in vitamin
Laboratory animal is divided into 2 groups of experiments with aforementioned:
1, matched group: 6, only feed equal-volume water for any medicine;
2, kidney-replenishing sperm-supplementing granule group (2.8g crude drug/kgBW is 10 times of plan clinical dosages), every day, oral administration was 1 time, 4 weeks of successive administration.Last administration 24h puts to death animal, takes out epididymis and deferent duct sperm, and the sperm processing method is the same, observes sperm morphology then.Form according to eupyrene sperm head, neck and tail three parts: the head acrosome is a sickleshaped, and smooth surface has tangible kiss end, is hook-shaped; Cervical region is short and straight; Afterbody is stage casing, principal piece and rear from top to bottom, and each section is smooth, does not have other accessory structures etc., identifies its rate of teratosperm.
The result: the unusual rate of matched group sperm morphology is very high, can reach 67.6 ± 6.5%.Abnormal spermium mainly shows as the head acrosome and is pure shape, and kiss end hook shape disappears, and the afterbody principal piece still keeps the endochylema droplet, or is the hair clip type deformity, shows sperm epididymis anacmesis.Administration group cacospermia rate obviously reduces (41.2 ± 5.2%).
Three, conclusion
1, kidney-replenishing sperm-supplementing granule can increase mouse sperm quantity and improve motility of sperm childhood significantly, but to essence
Sub-survival rate does not have obvious effect;
2, in vitro tests result shows that said preparation also can directly improve motility of sperm, and prolongs the sperm time-to-live;
3, kidney-replenishing sperm-supplementing granule can reduce the adult mice abnormal sperm rate significantly.
Description of drawings
Fig. 1 is the influence of kidney-replenishing sperm-supplementing granule to immaturity mouse sperm survival rate.Wherein 1 is matched group, 2 is kidney-replenishing sperm-supplementing granule group 1.4g crude drug/kg BW, 3 is kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kg BW, 4 is kidney-replenishing sperm-supplementing granule group 5.6g crude drug/kg BW, 5 is kidney-replenishing sperm-supplementing granule group 11.2g crude drug/kgBW, 6 for lacking kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kgBW of Solenognathus and Scolopendra, and 7 for singly there being kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kg BW of Solenognathus and Scolopendra, and 8 is kidney-replenishing sperm-supplementing granule group 6.7g crude drug/kg BW.
Fig. 2 is the influence of kidney-replenishing sperm-supplementing granule to immaturity mouse sperm vigor.Wherein 1 is matched group, 2 is kidney-replenishing sperm-supplementing granule group 1.4g crude drug/kg BW, 3 is kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kg BW, 4 is kidney-replenishing sperm-supplementing granule group 5.6g crude drug/kgBW, 5 is kidney-replenishing sperm-supplementing granule group 11.2g crude drug/kg BW, 6 for lacking kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kg BW of Solenognathus and Scolopendra, and 7 for singly there being kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kg BW of Solenognathus and Scolopendra, and 8 is kidney-replenishing sperm-supplementing granule group 6.7g crude drug/kg BW.
Fig. 3 is the influence of kidney-replenishing sperm-supplementing granule to immaturity mouse epididymis afterbody motility of sperm.
Fig. 4 is the influence of kidney-replenishing sperm-supplementing granule to immaturity mice sperm density.Wherein 1 is matched group, 2 is kidney-replenishing sperm-supplementing granule group 1.4g crude drug/kg BW, 3 is kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kg BW, 4 is kidney-replenishing sperm-supplementing granule group 5.6g crude drug/kg BW, 5 is kidney-replenishing sperm-supplementing granule group 11.2g crude drug/kg BW, 6 for lacking kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kg BW of Solenognathus and Scolopendra, and 7 for singly there being kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kg BW of Solenognathus and Scolopendra, and 8 is kidney-replenishing sperm-supplementing granule group 6.7g crude drug/kg BW.
Fig. 5 is the effect of kidney-replenishing sperm-supplementing granule to mice external sperm vigor.Wherein 1 is matched group, and 2 is kidney-replenishing sperm-supplementing granule group 2.2mg crude drug/kg BW, and 3 is kidney-replenishing sperm-supplementing granule group 4.4mg crude drug/kg BW, and 4 is kidney-replenishing sperm-supplementing granule group 8.8mg crude drug/kg BW.
Fig. 6 is a kidney-replenishing sperm-supplementing granule to the influence of adult mice sperm deformity due to the vitamin A for want of.Wherein 1 is matched group, and 2 is P＜0.001 kidney-replenishing sperm-supplementing granule group 2.8g crude drug/kg BW.
Kidney-replenishing sperm-supplementing granule of the present invention have tonify the kidney and support yang, the effect of Mi Gu granule, the treatment kidney yang declines due to the void Oligozoospermia, vigor is low, and dead smart too much male sterility has preferably curative effect. The present invention is take mouse as moving Object model carries out pharmaceutical research and shows kidney-replenishing sperm-supplementing granule to spermatogenesis, vigor and fall through standard ground Low abnormal sperm rate has obvious enhancing and improvement effect. Clinical testing tentatively shows, to treating because of few sperm Male sterility result for the treatment of due to disease, asthénospermie and the few sperm companion asthénospermie: total effective rate reaches 85.7%, Wherein cure rate is 20%, obvious effective rate 22.9%. This medicine valency is lower, is fit to civilian's medication, has bigger Promotional value.
The specific embodiment
Below in conjunction with embodiment the present invention is further described
Herba Cistanches 160～170g, Herba Epimedii 230～270g, Semen Cuscutae 160～170g, Fructus Lycii 160～170g, the Radix Astragali 230～270g, Solenognathus 58～70g, Fructus Psoraleae 160～170g, Radix Polygoni Multiflori Preparata 230～270g, Semen Ginkgo 160～170g, Scolopendra 13～22g.The patient each two, serve on 3 months once a day.
Some male patient of table 1 takes the therapeutic effect of kidney-replenishing sperm-supplementing granule
As seen from Table 1, total effective rate is 85.7%; Wherein oligospermatism and agnogenio sterility effective percentage are 100%, but routine number is less; Oligospermatism companion azoospermia take second place (92.3%); Azoospermia curative effect relatively poor (69.2%).Be 73.5 ± 30.3 total course of treatment.
Claims (1)
1, a kind of kidney-replenishing sperm-supplementing granule is characterized in that consisting of of its crude drug: Herba Cistanches 160～170g, Herba Epimedii 230～270g, Semen Cuscutae 160～170g, Fructus Lycii 160～170g, Solenognathus 58～70g, Fructus Psoraleae 160～170g, Radix Polygoni Multiflori Preparata 230～270g, the Radix Astragali 230～270g, Semen Ginkgo 160～170g, Scolopendra 13～22g.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200610050874A CN100594920C (en) | 2006-05-19 | 2006-05-19 | Bushen Zengjing Granules |
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| CN200610050874A CN100594920C (en) | 2006-05-19 | 2006-05-19 | Bushen Zengjing Granules |
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| CN1883612A CN1883612A (en) | 2006-12-27 |
| CN100594920C true CN100594920C (en) | 2010-03-24 |
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| CN200610050874A Expired - Fee Related CN100594920C (en) | 2006-05-19 | 2006-05-19 | Bushen Zengjing Granules |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108926629A (en) * | 2017-05-27 | 2018-12-04 | 成都贝爱特生物科技有限公司 | A kind of Chinese materia medica preparation improving human spermatogoa motility rate and egg penetration ability |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1562116A (en) * | 2004-03-26 | 2005-01-12 | 吉林敖东延边药业股份有限公司 | Chinese medicine preparation with invigorating kidney function |
| CN1682798A (en) * | 2004-09-06 | 2005-10-19 | 毛友昌 | Shenbao solid preparation good for kidney and its preparing method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1562116A (en) * | 2004-03-26 | 2005-01-12 | 吉林敖东延边药业股份有限公司 | Chinese medicine preparation with invigorating kidney function |
| CN1682798A (en) * | 2004-09-06 | 2005-10-19 | 毛友昌 | Shenbao solid preparation good for kidney and its preparing method |
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| CN1883612A (en) | 2006-12-27 |
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