CN100366189C - Edible farm and side line products - Google Patents
Edible farm and side line products Download PDFInfo
- Publication number
- CN100366189C CN100366189C CNB2006100020458A CN200610002045A CN100366189C CN 100366189 C CN100366189 C CN 100366189C CN B2006100020458 A CNB2006100020458 A CN B2006100020458A CN 200610002045 A CN200610002045 A CN 200610002045A CN 100366189 C CN100366189 C CN 100366189C
- Authority
- CN
- China
- Prior art keywords
- toad
- cutis bufonis
- test
- mouse
- micro powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000843 powder Substances 0.000 claims abstract description 16
- 235000013305 food Nutrition 0.000 claims abstract description 8
- 239000006227 byproduct Substances 0.000 claims abstract description 3
- 239000002245 particle Substances 0.000 claims description 11
- 235000012907 honey Nutrition 0.000 claims description 2
- 239000011122 softwood Substances 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 abstract description 9
- 241000699670 Mus sp. Species 0.000 abstract description 6
- 230000007665 chronic toxicity Effects 0.000 abstract description 6
- 231100000160 chronic toxicity Toxicity 0.000 abstract description 6
- 241000269420 Bufonidae Species 0.000 abstract description 2
- VOZHMAYHYHEWBW-NVOOAVKYSA-N Bufotalin Chemical compound C=1([C@@H]2[C@@]3(C)CC[C@@H]4[C@@]5(C)CC[C@H](O)C[C@H]5CC[C@H]4[C@@]3(O)C[C@@H]2OC(=O)C)C=CC(=O)OC=1 VOZHMAYHYHEWBW-NVOOAVKYSA-N 0.000 abstract description 2
- VOZHMAYHYHEWBW-UHFFFAOYSA-N Bufotalin Natural products CC(=O)OC1CC2(O)C3CCC4CC(O)CCC4(C)C3CCC2(C)C1C=1C=CC(=O)OC=1 VOZHMAYHYHEWBW-UHFFFAOYSA-N 0.000 abstract description 2
- 229930013930 alkaloid Natural products 0.000 abstract description 2
- 150000003797 alkaloid derivatives Chemical class 0.000 abstract description 2
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical class [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 abstract description 2
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 2
- 150000002475 indoles Chemical class 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 229910052711 selenium Inorganic materials 0.000 abstract 1
- 239000011669 selenium Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 18
- 241000699666 Mus <mouse, genus> Species 0.000 description 17
- 241001465754 Metazoa Species 0.000 description 9
- 230000003203 everyday effect Effects 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 210000003491 skin Anatomy 0.000 description 6
- 230000037396 body weight Effects 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 229920000832 Cutin Polymers 0.000 description 4
- 230000028327 secretion Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 2
- 230000003542 behavioural effect Effects 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 235000012631 food intake Nutrition 0.000 description 2
- 230000037308 hair color Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000010010 raising Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- VAJVDSVGBWFCLW-UHFFFAOYSA-N 3-Phenyl-1-propanol Chemical compound OCCCC1=CC=CC=C1 VAJVDSVGBWFCLW-UHFFFAOYSA-N 0.000 description 1
- 241000432767 Asparagus setaceus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- DYUQAZSOFZSPHD-UHFFFAOYSA-N Phenylpropyl alcohol Natural products CCC(O)C1=CC=CC=C1 DYUQAZSOFZSPHD-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000015177 dried meat Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 230000006266 hibernation Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000002435 venom Substances 0.000 description 1
- 210000001048 venom Anatomy 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to an edible agricultural byproduct, particularly to a horny membrane naturally exuviated by toads, i.e. a toad shell. The toad shell contains 49.57% of 18 kinds of amino acids, about 74% of protein, 0.15 mg/g of indoles total alkaloid, 0.1 mg/kg of selenium, only 0.03% of bufotalin, less than 1 ppm of arsenic salt and less than 5 ppm of heavy metal, and the indexes are all below the food limitation safety standard issued by the state ministry of health. The experiment on the maximum tolerable dose of the chronic toxicity of toad shell ultrafine powder on mice indicates that the toad shell is nontoxic to the mice.
Description
Technical field
The present invention relates to a kind of edible farm and side line products, this product is that the cutin clothing film under toad sloughs off naturally is a Cutis Bufonis specifically.
Background technology
It is the indisputable fact that is of long duration that toad has medical value, this point can find foundation in works such as " Mingyi Bielu ", " Chinese voluminous dictionary ", traditional method is to be used as medicine with dried toad (the dry toad corpse that expands in removing), the dried venom of toads (the white serum processed goods of ear rear gland of toad and skin of back secretion).But relevant cutin clothing film under sloughing off naturally with toad is that Cutis Bufonis is had no precedent report as the research of edible food.
Summary of the invention
One of main purpose of the present invention is the clothing film under sloughing off naturally with toad, and promptly Cutis Bufonis is not tortured the life of toad as food.Toad is distributed widely in China various places, and it just sloughs off epidermis fall once except that hibernation period at set intervals naturally, the entire process have about 5-10 minute, and healthy toad casts off a skin more than 20 time every year, and can cast off a skin hundreds of all one's life.This toad skin that falls of sloughing off naturally of toad is a cutin clothing film, is the metabolite that its physiological skin renewal is regenerated, and as agricultural byproducts applicatory, has not only effectively utilized the toad resource with this cutin clothing film, has also protected toad life.Contain rich in amino acid in the Cutis Bufonis, its kind reaches 18 kinds, total content 49.57%; Protein content accounts for about 74%; The indoles total alkaloid content is 0.15mg/g; Se content is 0.1mg/kg; And the content of bufotalin only is 0.03%, and the content of arsenic salt is lower than 1ppm, and the content of heavy metal is lower than 5ppm, and these indexs all are lower than food that health ministry the issues safety standard of limiting the quantity of; And Cutis Bufonis ultra micro powder shows that to the mtd test of mouse chronic toxicity Cutis Bufonis is nontoxic to mouse.
The wrinkle that the present invention will collect becomes one Cutis Bufonis to be placed on a carrier surface, must not being placed on wafts in the water washes, carrier is put into 30 to 70 degrees centigrade of drying box continuous dryings together with the Cutis Bufonis of carrier surface, make water content≤2.0%, remove impurity, be ground into 1200 order to 2000 purpose Cutis Bufonis ultra micro powder, divide in the polybag of packing into, sealing, sterilization gets required Cutis Bufonis.
The specific embodiment
1, the acquisition mode of Cutis Bufonis:
Toad in the Nature is placed on temporary supporting 1 to 3 day in the outdoor storage pond, holding pond that cleans up; Average per 3 to 6 hours with the clear water flushing once; Then the toad in the outdoor storage pond, holding pond is put into and indoorly clean sloughed off the clothing pond 1 to 7 day, average per 2 to 3 hours with the clear water rinsing once, the movable minimizing of toad this moment, be stupefied shape, whole skin glandular secretion thing increases, and is commonly called as " perspiration ", and it is moist bright that body surface seems.The slit that at first occurs longitudinal cracking on the midline backbone of back, increase along with the slit, toad begins to struggle, soon head, trunk and four limbs get out from the slit, catch alive toad with hand this moment, and the another hand just can collect Cutis Bufonis, wrinkles into one, the toad that lives is put into special-purpose tube, then the toad alive in the tube is put back to the Nature; Wrinkling into one Cutis Bufonis, to be placed on stainless steel wire online, must not be placed on rinsing in the water, Cutis Bufonis on stainless steel cloth and the silk screen is put into 30 to 70 degrees centigrade of drying box continuous dryings together, make water content≤2.0%, remove impurity, be ground into 1200 order to 2000 purpose Cutis Bufonis ultra micro powder, divide in the polybag of packing into, sealing, sterilization promptly can be used as food.
2, Cutis Bufonis amino acid analysis result report:
Measure amino acid whose kind and content in the Cutis Bufonis with the 835-50 of Hitachi type amino-acid analyzer, the result is as follows:
Amino acid analysis result (each amino acid content when following data are sample analysis in the consumption)
| Amino acid | ％ | Amino acid | ％ |
| Taurine TAU | 0.52 | Different bright He | 2.66 |
| Asparagus fern Asp | 2.48 | Bright Leu | 4.38 |
| The Thr of Soviet Union | 3.11 | Junket Tyr | 2.63 |
| Silk Ser | 9.80 | Phenylpropyl alcohol Phe | 2.26 |
| Paddy Glu | 1.47 | Bird ORN | 0.12 |
| Dried meat Pro | 4.07 | Rely Lys | 4.27 |
| Sweet Gly | 2.77 | Ammonia | |
| Third Ala | 0.47 | Group His | 1.15 |
| Guang Cys | 2.87 | Look Try | |
| Figured silk fabrics Val | 1.25 | Smart Arg | 3.29 |
| First sulphur Met |
3, Cutis Bufonis ultra micro powder is to the mtd test of mouse chronic toxicity
One. test objective
Observation Cutis Bufonis ultra micro powder particles (censorship number 050422) is to the chronic toxicity reaction of mouse.
Two. test material
1. tried thing:
Cutis Bufonis: 20 gram/boxes, be black clothing film bulk, gathering the base by Chanbao Organism Science and Technology Co., Ltd., Shanghai Cutis Bufonis provides, censorship number: 050422.The preparation of Cutis Bufonis ultra micro powder particles: get Cutis Bufonis 200 boxes, gross weight 4000 grams are ground into 1200 order to 2000 purpose Cutis Bufonis ultra micro powder, add honey and make softwood, pushed 5 mesh sieves, make the particle of grain of rice shape, put into baking oven continuous drying, make water content less than 5% less than 50 ℃, standby.
2. animal
100 of ICR mouse, male and female half and half, body weight 18 be to 20g, provided by west, the joint Shanghai of China and Britain pul-Bi Kai animal used as test Co., Ltd, and animal produces the quality certification number: Shanghai is moving closes the card word No. 163.Divide every cage 5 raisings.Raising temperature is 2 ℃ in 22 soil, and humidity is 60--75%.Feed is used the high binder of solid (west, Sino-British joint Shanghai pul-Bi Kai animal used as test Co., Ltd product) with mouse.Fasting is 12 hours before the test, can't help water.
Three. test method
Test in advance: Cutis Bufonis ultra micro powder particles is put into mouse with the food dish, and every day, twice mouse freely ate, and per kilogram mouse every day is from gulping down dosage 20,26g, male and female half and half, each observation of every day at upper and lower noon was once observed 14 days continuously, write down death state and the acute toxic reaction of animal in 14 days.Do not find animal dead, also not seeing has tangible toxic reaction.The mtd test of chronic toxicity: 60 of mouse, male and female half and half, press heap(ed) capacity 26g/kg, every day twice mouse Cutis Bufonis ultra micro powder particles of freely eating, the free diet of mouse behind the clothes Cutis Bufonis ultra micro powder particles, each observation of every day at upper and lower noon was once observed 90 days continuously, write down death state and the chronic toxicity reaction of animal in 90 days.
Four. result of the test
Mouse freely eats Cutis Bufonis ultra micro powder particles every day twice, each 13g/kg, and none example of mouse is dead, and hair color, body weight, behavioral activity, food consumption, ight soil proterties and secretion etc., does not all find tangible abnormal response.
| Observation index | 30 of male mices (test group) | 30 of female mices (test group) | 10 of male mices (control group) | 10 of female mices (control group) | ||||
| Before giving Cutis Bufonis | After giving Cutis Bufonis | Before giving Cutis Bufonis | After giving Cutis Bufonis | Before the test | After the test | Before the test | After the test | |
| Death toll (only) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Body weight (g) | 19.4 ± 0.5 | 29.9 ± 1.7 | 18.6 ± 0.5 | 28.7 ± 1.8 | 19.5 ± 0.5 | 26.8 ± 1.5 | 18.8 ± 0.5 | 26.6 ± 1.6 |
| Hair color | Normally | Normally | Normally | Normally | Normally | Normally | Normally | Normally |
| Behavioral activity | Normally | Normally | Normally | Normally | Normally | Normally | Normally | Normally |
| The food consumption | Normally | Normally | Normally | Normally | Normally | Normally | Normally | Normally |
| The ight soil proterties | Be shaped | Be shaped | Be shaped | Be shaped | Be shaped | Be shaped | Be shaped | Be shaped |
Five. the test brief summary
Mouse freely eats Cutis Bufonis ultra micro powder particles every day twice, and each 13g/kg does not find animal dead, and also not seeing has tangible chronic toxic reaction.
Six. conclusion (of pressure testing)
Freely the eat maximal tolerance dose of Cutis Bufonis ultra micro powder particles (censorship number 050422) of mouse is 26g/kg every day, and the prompting Cutis Bufonis is a kind of relatively edible farm and side line products of safety.None example of animal is dead in this test, and the mouse body weight all is significantly increased when off-test, and body weight is again apparently higher than control group, and growth conditions is splendid.Show that Cutis Bufonis has trophism preferably.
Claims (4)
1. clothing film under sloughing off naturally with toad, promptly Cutis Bufonis is as the purposes of food.
2. purposes as claimed in claim 1, wherein said food are agricultural byproducts.
3. purposes as claimed in claim 1, wherein Cutis Bufonis is a particle diameter 1200-2000 purpose ultra micro powder.
4. purposes as claimed in claim 3, wherein Cutis Bufonis ultra micro powder adds honey and makes softwood, pushes 5 mesh sieves, makes particle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100020458A CN100366189C (en) | 2006-01-24 | 2006-01-24 | Edible farm and side line products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100020458A CN100366189C (en) | 2006-01-24 | 2006-01-24 | Edible farm and side line products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1803019A CN1803019A (en) | 2006-07-19 |
| CN100366189C true CN100366189C (en) | 2008-02-06 |
Family
ID=36865274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100020458A Expired - Fee Related CN100366189C (en) | 2006-01-24 | 2006-01-24 | Edible farm and side line products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100366189C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688438A (en) * | 2012-02-01 | 2014-03-26 | 丰田自动车株式会社 | Electric storage system and method for judging state of electric storage block |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297761A (en) * | 1999-12-02 | 2001-06-06 | 王利忠 | Live toad exuviating liquid and multiple live toad exuviating technology |
| CN1465350A (en) * | 2002-07-05 | 2004-01-07 | 海门市康平蟾衣制药研究所 | Toad skin preparation and its production process |
-
2006
- 2006-01-24 CN CNB2006100020458A patent/CN100366189C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297761A (en) * | 1999-12-02 | 2001-06-06 | 王利忠 | Live toad exuviating liquid and multiple live toad exuviating technology |
| CN1465350A (en) * | 2002-07-05 | 2004-01-07 | 海门市康平蟾衣制药研究所 | Toad skin preparation and its production process |
Non-Patent Citations (1)
| Title |
|---|
| 蟾蜍貌虽丑治病属良药. 李秀英.药膳食疗，第9期. 2002 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688438A (en) * | 2012-02-01 | 2014-03-26 | 丰田自动车株式会社 | Electric storage system and method for judging state of electric storage block |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1803019A (en) | 2006-07-19 |
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