CH651476A5 - Antibodies against proteins - Google Patents
Antibodies against proteins Download PDFInfo
- Publication number
- CH651476A5 CH651476A5 CH1343/81A CH134381A CH651476A5 CH 651476 A5 CH651476 A5 CH 651476A5 CH 1343/81 A CH1343/81 A CH 1343/81A CH 134381 A CH134381 A CH 134381A CH 651476 A5 CH651476 A5 CH 651476A5
- Authority
- CH
- Switzerland
- Prior art keywords
- hli
- collection
- antibodies
- antibody
- interferon
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title claims description 16
- 108090000623 proteins and genes Proteins 0.000 title claims description 16
- 102000014150 Interferons Human genes 0.000 claims abstract description 110
- 108010050904 Interferons Proteins 0.000 claims abstract description 110
- 238000000034 method Methods 0.000 claims abstract description 23
- 210000004408 hybridoma Anatomy 0.000 claims abstract description 22
- 229940047124 interferons Drugs 0.000 claims abstract description 22
- 108020004511 Recombinant DNA Proteins 0.000 claims abstract description 18
- 239000007790 solid phase Substances 0.000 claims abstract description 12
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 11
- 238000000746 purification Methods 0.000 claims abstract description 11
- 238000001042 affinity chromatography Methods 0.000 claims abstract description 10
- 230000000890 antigenic effect Effects 0.000 claims abstract description 6
- 229940079322 interferon Drugs 0.000 claims description 88
- 238000012360 testing method Methods 0.000 claims description 21
- 102000006992 Interferon-alpha Human genes 0.000 claims description 16
- 108010047761 Interferon-alpha Proteins 0.000 claims description 16
- 210000004027 cell Anatomy 0.000 claims description 16
- 230000000840 anti-viral effect Effects 0.000 claims description 10
- 238000004140 cleaning Methods 0.000 claims description 8
- 241000699670 Mus sp. Species 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 5
- 201000000050 myeloid neoplasm Diseases 0.000 claims description 5
- 210000004989 spleen cell Anatomy 0.000 claims description 4
- 230000007910 cell fusion Effects 0.000 claims description 3
- 230000003612 virological effect Effects 0.000 claims description 3
- 239000013543 active substance Substances 0.000 claims description 2
- 238000012512 characterization method Methods 0.000 claims description 2
- 238000011161 development Methods 0.000 claims description 2
- 201000010099 disease Diseases 0.000 claims description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 238000011156 evaluation Methods 0.000 claims description 2
- 230000001771 impaired effect Effects 0.000 claims description 2
- 230000001613 neoplastic effect Effects 0.000 claims description 2
- 230000002285 radioactive effect Effects 0.000 claims description 2
- 230000000717 retained effect Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 230000001225 therapeutic effect Effects 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 1
- 230000027455 binding Effects 0.000 description 32
- 238000009739 binding Methods 0.000 description 31
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 26
- 239000002953 phosphate buffered saline Substances 0.000 description 26
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
- 230000000694 effects Effects 0.000 description 17
- 229940098773 bovine serum albumin Drugs 0.000 description 16
- 239000000243 solution Substances 0.000 description 14
- 239000000499 gel Substances 0.000 description 12
- 210000002966 serum Anatomy 0.000 description 11
- 241000699666 Mus <mouse, genus> Species 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 239000011780 sodium chloride Substances 0.000 description 9
- 241000894007 species Species 0.000 description 9
- 238000011534 incubation Methods 0.000 description 8
- 230000005764 inhibitory process Effects 0.000 description 7
- 239000004800 polyvinyl chloride Substances 0.000 description 7
- 229920000915 polyvinyl chloride Polymers 0.000 description 7
- 239000000872 buffer Substances 0.000 description 6
- 238000006386 neutralization reaction Methods 0.000 description 6
- 210000002381 plasma Anatomy 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 229920004890 Triton X-100 Polymers 0.000 description 5
- 239000013504 Triton X-100 Substances 0.000 description 5
- 230000004927 fusion Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 206010003445 Ascites Diseases 0.000 description 4
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 3
- 239000005695 Ammonium acetate Substances 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 229940043376 ammonium acetate Drugs 0.000 description 3
- 235000019257 ammonium acetate Nutrition 0.000 description 3
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000003472 neutralizing effect Effects 0.000 description 3
- 230000009871 nonspecific binding Effects 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 241001467552 Mycobacterium bovis BCG Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 238000007912 intraperitoneal administration Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- QIVUCLWGARAQIO-OLIXTKCUSA-N (3s)-n-[(3s,5s,6r)-6-methyl-2-oxo-1-(2,2,2-trifluoroethyl)-5-(2,3,6-trifluorophenyl)piperidin-3-yl]-2-oxospiro[1h-pyrrolo[2,3-b]pyridine-3,6'-5,7-dihydrocyclopenta[b]pyridine]-3'-carboxamide Chemical compound C1([C@H]2[C@H](N(C(=O)[C@@H](NC(=O)C=3C=C4C[C@]5(CC4=NC=3)C3=CC=CN=C3NC5=O)C2)CC(F)(F)F)C)=C(F)C=CC(F)=C1F QIVUCLWGARAQIO-OLIXTKCUSA-N 0.000 description 1
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- PQBZDGDVGDHNHR-UHFFFAOYSA-N 3-ethyl-2h-1,3-benzothiazole;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2N(CC)CSC2=C1 PQBZDGDVGDHNHR-UHFFFAOYSA-N 0.000 description 1
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- LPXQRXLUHJKZIE-UHFFFAOYSA-N 8-azaguanine Chemical compound NC1=NC(O)=C2NN=NC2=N1 LPXQRXLUHJKZIE-UHFFFAOYSA-N 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 102000012745 Immunoglobulin Subunits Human genes 0.000 description 1
- 108010079585 Immunoglobulin Subunits Proteins 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 229920002873 Polyethylenimine Polymers 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 229960003896 aminopterin Drugs 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000001064 anti-interferon Effects 0.000 description 1
- 230000009833 antibody interaction Effects 0.000 description 1
- 229960000190 bacillus calmette–guérin vaccine Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- VDQQXEISLMTGAB-UHFFFAOYSA-N chloramine T Chemical compound [Na+].CC1=CC=C(S(=O)(=O)[N-]Cl)C=C1 VDQQXEISLMTGAB-UHFFFAOYSA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000011097 chromatography purification Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000009137 competitive binding Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 150000002009 diols Chemical class 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 210000004013 groin Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- PYLWMHQQBFSUBP-UHFFFAOYSA-N monofluorobenzene Chemical compound FC1=CC=CC=C1 PYLWMHQQBFSUBP-UHFFFAOYSA-N 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 210000000066 myeloid cell Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 230000003617 peroxidasic effect Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229940057838 polyethylene glycol 4000 Drugs 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000012475 sodium chloride buffer Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 235000011008 sodium phosphates Nutrition 0.000 description 1
- 229940054269 sodium pyruvate Drugs 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- YODZTKMDCQEPHD-UHFFFAOYSA-N thiodiglycol Chemical compound OCCSCCO YODZTKMDCQEPHD-UHFFFAOYSA-N 0.000 description 1
- 229950006389 thiodiglycol Drugs 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 229960001479 tosylchloramide sodium Drugs 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/249—Interferons
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
- G01N33/6866—Interferon
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (24)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH1343/81A CH651476A5 (en) | 1981-02-27 | 1981-02-27 | Antibodies against proteins |
AU80772/82A AU561649B2 (en) | 1981-02-27 | 1982-02-24 | Antibodies to proteins |
FR8203018A FR2500754B1 (fr) | 1981-02-27 | 1982-02-24 | Anticorps monoclonaux |
IL65105A IL65105A0 (en) | 1981-02-27 | 1982-02-25 | Antibodies to proteins |
PH26917A PH18323A (en) | 1981-02-27 | 1982-02-25 | Antibodies to proteins |
LU83978A LU83978A1 (de) | 1981-02-27 | 1982-02-25 | Antikoerper gegen proteine |
DE19823206743 DE3206743A1 (de) | 1981-02-27 | 1982-02-25 | Antikoerper gegen proteine |
IT19854/82A IT1150201B (it) | 1981-02-27 | 1982-02-25 | Anticorpi contro proteine |
ZA821271A ZA821271B (en) | 1981-02-27 | 1982-02-25 | Antibodies to proteins |
NZ199833A NZ199833A (en) | 1981-02-27 | 1982-02-25 | Monoclonal antibodies directed against human leucocyte interferon(hli);purification of hli |
DK86382A DK86382A (da) | 1981-02-27 | 1982-02-26 | Antistoffer mod proteiner |
JP57030471A JPS57197224A (en) | 1981-02-27 | 1982-02-26 | Human leucocyte interferon antibody |
GB08205667A GB2111527B (en) | 1981-02-27 | 1982-02-26 | Monoclonal antibodies to human leucocyte interferon |
CA000397155A CA1213226A (fr) | 1981-02-27 | 1982-02-26 | Anticorps contre l'interferon des leucocytes humains |
NL8200786A NL8200786A (nl) | 1981-02-27 | 1982-02-26 | Antilichamen tegen proteine. |
IE422/82A IE52269B1 (en) | 1981-02-27 | 1982-02-26 | Antibodies to proteins |
AT0074082A ATA74082A (de) | 1981-02-27 | 1982-02-26 | Festphasen-sandwich-test |
IE1772/86A IE52270B1 (en) | 1981-02-27 | 1982-02-26 | Monoclonal antibody to hli and its use for purifying hli |
BE0/207424A BE892296A (fr) | 1981-02-27 | 1982-02-26 | Anticorps monoclonaux |
SE8201226A SE8201226L (sv) | 1981-02-27 | 1982-03-02 | Antikroppar mot proteiner |
GB08504271A GB2160544B (en) | 1981-02-27 | 1985-02-19 | Monoclonal antibody to hli and its use for purifying hli |
AU69844/87A AU590688B2 (en) | 1981-02-27 | 1987-03-09 | Monoclonal antibody to HLI and its use for purifying HLI |
AT179288A ATA179288A (de) | 1981-02-27 | 1988-07-11 | Antikoerper gegen proteine |
SE9000931A SE9000931L (sv) | 1981-02-27 | 1990-03-15 | Antikroppar mot proteiner |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH1343/81A CH651476A5 (en) | 1981-02-27 | 1981-02-27 | Antibodies against proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
CH651476A5 true CH651476A5 (en) | 1985-09-30 |
Family
ID=4208260
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CH1343/81A CH651476A5 (en) | 1981-02-27 | 1981-02-27 | Antibodies against proteins |
Country Status (4)
Country | Link |
---|---|
JP (1) | JPS57197224A (fr) |
BE (1) | BE892296A (fr) |
CH (1) | CH651476A5 (fr) |
ZA (1) | ZA821271B (fr) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59172496A (ja) * | 1983-03-18 | 1984-09-29 | Kyowa Hakko Kogyo Co Ltd | モノクロ−ナル抗体 |
JPS6041697A (ja) * | 1983-08-15 | 1985-03-05 | Asahi Chem Ind Co Ltd | 活性蛋白質誘導体の新規合成法 |
JPH0794475B2 (ja) * | 1983-08-16 | 1995-10-11 | 雪印乳業株式会社 | 純粋なエリスロポエチンの製造法 |
-
1981
- 1981-02-27 CH CH1343/81A patent/CH651476A5/de not_active IP Right Cessation
-
1982
- 1982-02-25 ZA ZA821271A patent/ZA821271B/xx unknown
- 1982-02-26 JP JP57030471A patent/JPS57197224A/ja active Pending
- 1982-02-26 BE BE0/207424A patent/BE892296A/fr unknown
Also Published As
Publication number | Publication date |
---|---|
ZA821271B (en) | 1983-01-26 |
BE892296A (fr) | 1982-08-26 |
JPS57197224A (en) | 1982-12-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE3586536T3 (de) | Monoklonaler Antikörper gegen ein Cytotoxin, denselben erzeugendes Hybridoma und Verfahren für die Herstellung des gereinigten Cytotoxin. | |
EP0158599B1 (fr) | Anticorps monoclonaux et cellules hybridomes, procédé pour leur préparation et leur application | |
DE68920693T2 (de) | Gefriergetrocknete Zusammensetzung, die ein mit Meerrettichperoxydase markiertes Fab'-Fragment eines gegen menschliches Beta-Interferon gerichteten Antikörpers und Trehalose enthält; EIA Kit, der diese Zusammensetzung enthält. | |
DE69034249T2 (de) | Monoklonale Antikörper spezifisch für den zytotoxischen Lymphozyten-Reifefaktor | |
DE3689734T2 (de) | Gereinigter immunoglobulinbezüglicher Faktor, monoklonale Antikörper, Hybridoma-Zellinien, Verfahren und Verwendungen. | |
US4504586A (en) | Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1 | |
DE68925935T2 (de) | Antikörper gegen Interleukin-1-beta | |
EP0260610A2 (fr) | Anticorps monoclonaux contre le facteur nécrotique tumoral humain (TNF) et leur utilisation | |
DE3486453T2 (de) | Monoklonale Antikörper gegen neue Faktor-VIII Gerinnungspolypeptide | |
EP1888642B1 (fr) | Partenaires de liaison du facteur de croissance placentaire, notamment anticorps diriges contre le facteur de croissance placentaire, leur production et leur utilisation | |
EP0162812B1 (fr) | Lymphokine sous forme pure, anticorps monoclonaux, lignées de cellules hybridomes, procédés et applications | |
EP0122628A2 (fr) | Anticorps monoclonaux contre l'interféron immun humain | |
EP0547059B1 (fr) | Anticorps specifique a la pancreas-elastase-1, un procede pour son obtention et un kit de test contenant de tels anticorps | |
DE3206743A1 (de) | Antikoerper gegen proteine | |
DE3780256T2 (de) | Anti-pci-monoklonaler antikoerper. | |
CH651476A5 (en) | Antibodies against proteins | |
EP0262571B1 (fr) | Anticorps monoclonaux contre IFN-oméga, leur procédé de préparation et leur application pour la purification et détection d'IFN-oméga | |
DE3850993T2 (de) | Monoklonaler Antikörper spezifisch gegen humane Pankreas-Phospholipase-A2. | |
EP0470565A1 (fr) | Peptides avec une déterminante antigène caractéristique pour alpha 1-microglobuline | |
DE68917797T2 (de) | Gegen menschliches Fibrinopepid A spezifische monoklonale Antikörper. | |
DE68911574T2 (de) | Reagenzsystem zur Bestimmung des Komplexes des menschlichen Plasminogenaktivator-Inhibitors und des menschlichen Gewebeplasminogenaktivators und Testsatz dafür. | |
US4803167A (en) | Monoclonal antidigoxtin antibodies | |
DE3687542T2 (de) | Monoklonaler antikoerper gegen humanes protein c. | |
DE19529026C2 (de) | Monoklonale Antikörper gegen humanes Interleukin-10 | |
DE3855007T2 (de) | METHODE ZUR REINIGUNG REKOMBINANTER t-PA VON DEN IHREN ENTSPRECHENDEN WIRTSZELLPROTEINEN |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUE | Assignment |
Owner name: F. HOFFMANN-LA ROCHE AG |
|
PL | Patent ceased | ||
PL | Patent ceased |