CA2430527A1 - Mass spectrometer - Google Patents
Mass spectrometer Download PDFInfo
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- CA2430527A1 CA2430527A1 CA002430527A CA2430527A CA2430527A1 CA 2430527 A1 CA2430527 A1 CA 2430527A1 CA 002430527 A CA002430527 A CA 002430527A CA 2430527 A CA2430527 A CA 2430527A CA 2430527 A1 CA2430527 A1 CA 2430527A1
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- mass spectrometer
- transient
- fragmentation device
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- 150000002500 ions Chemical class 0.000 claims abstract 66
- 238000006243 chemical reaction Methods 0.000 claims abstract 7
- 238000001816 cooling Methods 0.000 claims abstract 3
- 238000013467 fragmentation Methods 0.000 claims 56
- 238000006062 fragmentation reaction Methods 0.000 claims 56
- 230000001052 transient effect Effects 0.000 claims 56
- 230000007423 decrease Effects 0.000 claims 15
- 239000007789 gas Substances 0.000 claims 6
- 238000000034 method Methods 0.000 claims 6
- 230000004888 barrier function Effects 0.000 claims 4
- 238000011144 upstream manufacturing Methods 0.000 claims 3
- 238000013016 damping Methods 0.000 claims 2
- 238000003795 desorption Methods 0.000 claims 2
- 238000010265 fast atom bombardment Methods 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 238000009616 inductively coupled plasma Methods 0.000 claims 2
- 239000011261 inert gas Substances 0.000 claims 2
- 238000005040 ion trap Methods 0.000 claims 2
- 238000004949 mass spectrometry Methods 0.000 claims 2
- 102100022704 Amyloid-beta precursor protein Human genes 0.000 claims 1
- 238000004252 FT/ICR mass spectrometry Methods 0.000 claims 1
- 101000823051 Homo sapiens Amyloid-beta precursor protein Proteins 0.000 claims 1
- DZHSAHHDTRWUTF-SIQRNXPUSA-N amyloid-beta polypeptide 42 Chemical compound C([C@@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O)[C@@H](C)CC)C(C)C)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC(O)=O)C(C)C)C(C)C)C1=CC=CC=C1 DZHSAHHDTRWUTF-SIQRNXPUSA-N 0.000 claims 1
- 238000000065 atmospheric pressure chemical ionisation Methods 0.000 claims 1
- 230000004323 axial length Effects 0.000 claims 1
- 238000000451 chemical ionisation Methods 0.000 claims 1
- 230000005684 electric field Effects 0.000 claims 1
- 238000004992 fast atom bombardment mass spectroscopy Methods 0.000 claims 1
- 238000001698 laser desorption ionisation Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 claims 1
- 238000005036 potential barrier Methods 0.000 claims 1
- 230000001360 synchronised effect Effects 0.000 claims 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/06—Electron- or ion-optical arrangements
- H01J49/062—Ion guides
- H01J49/065—Ion guides having stacked electrodes, e.g. ring stack, plate stack
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
- H01J49/0045—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn characterised by the fragmentation or other specific reaction
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0468—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components with means for heating or cooling the sample
- H01J49/0481—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components with means for heating or cooling the sample with means for collisional cooling
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Electron Tubes For Measurement (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
A mass spectrometer is disclosed comprising a gas collision cell, reaction cell or collisional cooling cell 1 comprising a plurality of electrodes 2. DC
potentials are progressively applied to the cell 1 so that ions are urged along the cell 1.
potentials are progressively applied to the cell 1 so that ions are urged along the cell 1.
Claims (82)
1. A mass spectrometer comprising:
a fragmentation device comprising a plurality of electrodes wherein, in use, one or more transient DC
voltages or one or more transient DC voltage waveforms are progressively applied to said electrodes so that ions are urged along said fragmentation device.
a fragmentation device comprising a plurality of electrodes wherein, in use, one or more transient DC
voltages or one or more transient DC voltage waveforms are progressively applied to said electrodes so that ions are urged along said fragmentation device.
2. A mass spectrometer as claimed in claim 1, wherein in use an axial voltage gradient along at least a portion of the length of said fragmentation device varies with time whilst ions are being transmitted through said fragmentation device.
3. A mass spectrometer as claimed in claim 1 or 2, wherein said fragmentation device comprises at least a first electrode held at a first reference potential, a second electrode held at a second reference potential, and a third electrode held at a third reference potential, wherein:
at a first time t1 a first DC voltage is supplied to said first electrode so that said first electrode is held at a first potential above or below said first reference potential;
at a second later time t2 a second DC voltage is supplied to said second electrode so that said second electrode is held at a second potential above or below said second reference potential; and at a third later time t3 a third DC voltage is supplied to said third electrode so that said third electrode is held at a third potential above or below said third reference potential.
at a first time t1 a first DC voltage is supplied to said first electrode so that said first electrode is held at a first potential above or below said first reference potential;
at a second later time t2 a second DC voltage is supplied to said second electrode so that said second electrode is held at a second potential above or below said second reference potential; and at a third later time t3 a third DC voltage is supplied to said third electrode so that said third electrode is held at a third potential above or below said third reference potential.
4. A mass spectrometer as claimed in claim 3, wherein:
at said first time t1 said second electrode is at said second reference potential and said third electrode is at said thud reference potential at said second time t2 said first electrode is at said first potential and said third electrode is at said third reference potential; and at said third time t3 said first electrode is at said first potential and said second electrode is at said second potential.
at said first time t1 said second electrode is at said second reference potential and said third electrode is at said thud reference potential at said second time t2 said first electrode is at said first potential and said third electrode is at said third reference potential; and at said third time t3 said first electrode is at said first potential and said second electrode is at said second potential.
5. A mass spectrometer as claimed in claim 3, wherein:
at said first time t1 said second electrode is at said second reference potential and said third electrode is at said third reference potential;
at said second time t2 said first electrode is no longer supplied with said first DC voltage so that said first electrode is returned to said first reference potential and said third electrode is at said third reference potential; and at said third time t3 said second electrode is no longer supplied with said second DC voltage so that said second electrode is returned to said second reference potential and said first electrode is at said first reference potential.
at said first time t1 said second electrode is at said second reference potential and said third electrode is at said third reference potential;
at said second time t2 said first electrode is no longer supplied with said first DC voltage so that said first electrode is returned to said first reference potential and said third electrode is at said third reference potential; and at said third time t3 said second electrode is no longer supplied with said second DC voltage so that said second electrode is returned to said second reference potential and said first electrode is at said first reference potential.
6. A mass spectrometer as claimed in claim 3, 4 or 5, wherein said first, second and third reference potentials are substantially the same.
7. A mass spectrometer as claimed in any of claims 3-6, wherein said first, second and third DC voltages are substantially the same.
8. A mass spectrometer as claimed in any of claims 3-7, wherein said first, second and third potentials are substantially the same.
9. A mass spectrometer as claimed in any preceding claim, wherein said fragmentation device comprises 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 16, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or >30 segments, wherein each segment comprises 1, 2, 3, 4, 5, 6, 7, 6, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or >30 electrodes and wherein the electrodes in a segment are maintained at substantially the same DC potential.
10. A mass spectrometer as claimed in claim 9, wherein a plurality of segments are maintained at substantially the same DC potential.
11. A mass spectrometer as claimed in claim 9 or 10, wherein each segment is maintained at substantially the same DC potential as the subsequent nth segment wherein n is 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 16, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or >30.
12. A mass spectrometer as claimed in any preceding claim, wherein ions are confined radially within said fragmentation device by an AC or RF electric field.
13. A mass spectrometer as claimed in any preceding claim, wherein ions are radially confined within said fragmentation device in a pseudo-potential well and are constrained axially by a real potential barrier or well.
14. A mass spectrometer as claimed in any preceding claim, wherein the transit time of ions through said fragmentation device is selected from the group consisting of: (i) less than or equal to 20 ms; (ii) less than or equal to 10 ms; (iii) less than or equal to ms; (iv) less than or equal to 1 ms; and (v) less than or equal to 0.5 ms.
15. A mass spectrometer as claimed in any preceding claim, wherein at least 50%, 60%, 70%, 80%, 90% or 95%
of the ions entering said fragmentation device are arranged to have, in use, an energy greater than or equal to 10 ev for a singly charged ion or greater than or equal to 20 eV for a doubly charged ion such that said ions are caused to fragment.
of the ions entering said fragmentation device are arranged to have, in use, an energy greater than or equal to 10 ev for a singly charged ion or greater than or equal to 20 eV for a doubly charged ion such that said ions are caused to fragment.
16. A mass spectrometer as claimed in any preceding claim, wherein at least 50%, 60%, 70%, 80%, 90% or 95%
of the ions entering said fragmentation device are arranged to fragment upon colliding with collision gas within said fragmentation device.
of the ions entering said fragmentation device are arranged to fragment upon colliding with collision gas within said fragmentation device.
17. A mass spectrometer as claimed in any preceding claim, wherein said fragmentation device is maintained at a pressure selected from the group consisting of: (i) greater than or equal to 0.0001 mbar; (ii) greater than or equal to 0.0005 mbar; (iii) greater than or equal to 0.001 mbar; (iv) greater than or equal to 0.005 mbar;
(v) greater than or equal to 0.01 mbar; (vi) greater than or equal to 0.05 mbar; (vii) greater than or equal to 0.1 mbar; (viii) greater than or equal to 0.5 mbar;
(ix) greater than or equal to 1 mbar; (x) greater than or equal to 5 mbar; and (xi) greater than or equal to 10 mbar.
(v) greater than or equal to 0.01 mbar; (vi) greater than or equal to 0.05 mbar; (vii) greater than or equal to 0.1 mbar; (viii) greater than or equal to 0.5 mbar;
(ix) greater than or equal to 1 mbar; (x) greater than or equal to 5 mbar; and (xi) greater than or equal to 10 mbar.
18. A mass spectrometer as claimed in any preceding claim, wherein said fragmentation device is maintained at a pressure selected from the group consisting of: (i) less than or equal to 10 mbar; (ii) less than or equal to 5 mbar; (iii) less than or equal to 1 mbar: (iv) less than or equal to 0.5 mbar; (v) less than or equal to 0.1 mbar; (vi) less than or equal to 0.05 mbar; (vii) less than or equal to 0.01 mbar; (viii) less than or equal to 0.005 mbar; (ix) less than or equal to 0.001 mbar; (x) less than or equal to 0.0005 mbar; and (xi) less than or equal to 0.0001 mbar.
19. A mass Spectrometer as claimed in any preceding claim, wherein said fragmentation device is maintained, in use, at a pressure selected from the group consisting of; (i) between 0.0001 and 10 mbar; (ii) between 0.0001 and 1 mbar; (iii) between 0.0001 and 0.1 mbar; (iv) between 0.0001 and 0.01 mbar; (v) between 0.0001 and 0.001 mbar; (vi) between 0.001 and 10 mbar; (vii) between 0.001 and 1 mbar; (viii) between 0.001 and 0.1 mbar; (ix) between 0.001 and 0.01 mbar; (x) between 0.01 and l0 mbar; (xi) between 0.01 and 1 mbar; (xii) between 0.01 and 0.1 mbar; (xiii) between 0.1 and 10 mbar; (xiv) between 0.1 and 1 mbar; and (xv) between 1 and 10 mbar.
20. A mass spectrometer as claimed in any preceding claim, wherein said fragmentation device is maintained, in use, at a pressure such that a viscous drag is imposed upon ions passing through said fragmentation device.
21. A mass spectrometer as claimed in any preceding claim, wherein in use said one or more transient DC
voltages or said one or more transient DC voltage waveforms are initially provided at a first axial position and are then subsequently provided at second, then third different axial positions along said fragmentation device.
voltages or said one or more transient DC voltage waveforms are initially provided at a first axial position and are then subsequently provided at second, then third different axial positions along said fragmentation device.
22. A mass spectrometer as claimed in any preceding claim, wherein said one or more transient DC voltages or said one or more transient DC voltage waveforms move in use from one end of said fragmentation device to another end of said fragmentation device so that ions are urged along said fragmentation device.
23. A mass spectrometer as claimed in any preceding claim, wherein said one or more transient DC voltages create: (i) a potential hill or barrier; (ii) a potential well; (iii) multiple potential hills or barriers; (iv) multiple potential wells; (v) a combination of a potential hill or barrier and a potential well; or (vi) a combination of multiple potential hills or barriers and multiple potential wells.
24. A mass spectrometer in any preceding claim, wherein said one or more transient DC voltage waveforms comprise a repeating waveform.
25. A mass spectrometer as claimed in claim 24, wherein said one or more transient DC voltage waveforms comprise a square wave.
26. A mass spectrometer as claimed in any preceding claim, wherein the amplitude of said one or more transient DC voltages or said one or more transient DC
voltage waveforms remains substantially constant with time.
voltage waveforms remains substantially constant with time.
27. A mass spectrometer as claimed in any of claims 1-25, wherein the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms varies with time.
28. A mass spectrometer as claimed in claim 27, wherein the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms either: (i) increases with time; (ii) increases then decreases with time; (iii) decreases with times or (iv) decreases then increases with time.
29. A mass spectrometer as claimed in claim 28, wherein said fragmentation device comprises an upstream entrance region, a downstream exit region and an intermediate region, wherein:
in said entrance region the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms has a first amplitude;
in said intermediate region the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms has a second amplitude;
and in said exit region the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms has a third amplitude.
in said entrance region the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms has a first amplitude;
in said intermediate region the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms has a second amplitude;
and in said exit region the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms has a third amplitude.
30. A mass spectrometer as claimed in claim 29, wherein the entrance and/or exit region comprise a proportion of the total axial length of said fragmentation device selected from the group consisting of: (i) < 5%; (ii) 5-10%: (iii) 10-15%; (iv) 15-20%; (v) 20-25%; (vi) 25-30%;
(vii) 30-35%; (viii) 35-40%; and (ix) 40-45%.
(vii) 30-35%; (viii) 35-40%; and (ix) 40-45%.
31. A mass spectrometer as claimed in claim 29 or 30, wherein said first and/or third amplitudes are substantially zero and said second amplitude is substantially non-zero.
32. A mass spectrometer as claimed in claim 29, 30 or 31, wherein said second amplitude is larger than said first amplitude and/or said second amplitude is larger than said third amplitude.
33. A mass spectrometer as claimed in any preceding claim, wherein said one or more transient DC voltages or said one or more transient DC voltage waveforms pass in use along said fragmentation device with a first velocity.
34. A mass spectrometer as claimed in claim 33, wherein said first velocity: (i) remains substantially constant;
(ii) varies; (iii) increases; (iv) increases then decreases; (v) decreases; (vi) decreases then increases;
(vii) reduces to substantially zero; (viii) reverses direction; or (ix) reduces to substantially zero and then reverses direction.
(ii) varies; (iii) increases; (iv) increases then decreases; (v) decreases; (vi) decreases then increases;
(vii) reduces to substantially zero; (viii) reverses direction; or (ix) reduces to substantially zero and then reverses direction.
35. A mass spectrometer as claimed in any preceding claim, wherein said one or more transient DC voltages or said one or more transient DC voltage waveforms cause ions within said fragmentation device to pass along said fragmentation device with a second velocity.
36. A mass spectrometer as claimed in claim 35, wherein the difference between said first velocity and said second velocity is less than or equal to 100 m/s, 90 m/s, 80 m/s, 70 m/s, 60 m/s, 50 m/s, 40 m/s, 30 m/s, 20 m/s, 10 m/s, 5 m/s or 1 m/s.
37. A mass spectrometer as claimed in any of claims 33-36, wherein said first velocity is selected from the group consisting of: (i) 10-250 m/s; (ii) 250-500 m/s;
(iii) 500-750 m/s; (iv) 750-1000 m/s; (v) 1000-1250 m/s;
(vi) 1250-1500 m/s; (vii) 1500-1750 m/s; (viii,) 1750-2000 m/s; (ix) 2000-2250 m/s; (x) 2250-2500 m/s; (xi) 2500-2750 m/s; (xii) 2750-3000 m/s; (xiii) 3000-3250 m/s; (xiv) 3250-3500 m/s; (xv) 3500-3750 m/s; (xvi) 3750-4000 m/s; (xvii) 4000-4250 m/s; (xviii) 4250-4500 m/s; (xix) x500-4750 m/s; (xx) 4750-5000 m/s; and (xxi) > 5000 m/s.
(iii) 500-750 m/s; (iv) 750-1000 m/s; (v) 1000-1250 m/s;
(vi) 1250-1500 m/s; (vii) 1500-1750 m/s; (viii,) 1750-2000 m/s; (ix) 2000-2250 m/s; (x) 2250-2500 m/s; (xi) 2500-2750 m/s; (xii) 2750-3000 m/s; (xiii) 3000-3250 m/s; (xiv) 3250-3500 m/s; (xv) 3500-3750 m/s; (xvi) 3750-4000 m/s; (xvii) 4000-4250 m/s; (xviii) 4250-4500 m/s; (xix) x500-4750 m/s; (xx) 4750-5000 m/s; and (xxi) > 5000 m/s.
38. A mass spectrometer as claimed in any of claims 35-37, wherein said second velocity is selected from the group consisting of: (i) 10-250 m/s; (ii) 250-500 m/s;
(iii) 500-750 m/s; (iv) 750-1000 m/s; (v) 1000-1250 m/s;
(vi) 1250-1500 m/s; (vii) 1500-1750 m/s; (viii) 1750-2000 m/s; (ix) 2000-2250 m/s; (x) 2250-2500 m/s; (xi) 2500-2750 m/s; (xii) 2750-3000 m/s; (xiii) 3000-3250 m/s; (xiv) 3250-3500 m/s; (xv) 3500-3750 m/s; (xvi) 3750-4000 m/s; (xvii) 4000-4250 m/s; (xviii) 4250-4500 m/s; (xix) 4500-4750 m/s; (xx) 4750-5000 m/s; and (xxi) > 5000 m/s.
(iii) 500-750 m/s; (iv) 750-1000 m/s; (v) 1000-1250 m/s;
(vi) 1250-1500 m/s; (vii) 1500-1750 m/s; (viii) 1750-2000 m/s; (ix) 2000-2250 m/s; (x) 2250-2500 m/s; (xi) 2500-2750 m/s; (xii) 2750-3000 m/s; (xiii) 3000-3250 m/s; (xiv) 3250-3500 m/s; (xv) 3500-3750 m/s; (xvi) 3750-4000 m/s; (xvii) 4000-4250 m/s; (xviii) 4250-4500 m/s; (xix) 4500-4750 m/s; (xx) 4750-5000 m/s; and (xxi) > 5000 m/s.
39. A mass spectrometer as claimed in claim 35, wherein said second velocity is substantially the same as said first velocity.
40. A mass spectrometer as claimed in any preceding claim, wherein said one or more transient DC voltages or said one or more transient DC voltage waveforms has a frequency, and wherein said frequency: (i) remains substantially constant; (ii) varies; (iii) increases;
(iv) increases then decreases; (v) decreases; or (vi) decreases then increases.
(iv) increases then decreases; (v) decreases; or (vi) decreases then increases.
41. A mass spectrometer as claimed in any preceding claim, wherein said one or more transient DC voltages or said one or more transient DC voltage waveforms has a wavelength, and wherein said wavelength: (i) remains substantially constant; (ii) varies; (iii) increases;
(iv) increases then decreases; (v) decreases; or (vi) decreases then increases.
(iv) increases then decreases; (v) decreases; or (vi) decreases then increases.
42. A mass spectrometer as claimed in any preceding claim, wherein two or more transient DC voltages or two or more transient DC waveforms are arranged to pass simultaneously along said fragmentation device.
43. A mass spectrometer as claimed in claim 42, wherein said two or more transient DC voltages or said two or more transient DC waveforms are arranged to move: (i) in the same direction; (ii) in opposite directions; (iii) towards each other; (iv) away from each other.
44. A mass spectrometer as claimed in any preceding claim, wherein said one or more transient DC voltages or said one or more transient DC waveforms are repeatedly generated and passed in use along said fragmentation device, and wherein the frequency of generating said one or more transient DC voltages or said one or more transient DC voltage waveforms: (i) remains substantially constant; (ii) varies; (iii) increases;
(iv) increases then decreases; (v) decreases; or (vi) decreases then increases.
(iv) increases then decreases; (v) decreases; or (vi) decreases then increases.
45. A mass spectrometer as claimed in any preceding claim, wherein in use a continuous beam of ions is received at an entrance to said fragmentation device.
46. A mass spectrometer as claimed in any of claims 1-44, wherein in use packets of ions are received at an entrance to said fragmentation device.
47. A mass spectrometer as claimed in any preceding claim, wherein in use pulses of ions emerge from an exit of said fragmentation device.
48. A mass spectrometer as claimed in claim 47, further comprising an ion detector, said ion detector being arranged to be substantially phase locked in use with the pulses of ions emerging from the exit of the fragmentation device.
49. A mass spectrometer as claimed in claim 47 or 48, further comprising a Time of Flight mass analyser comprising an electrode for injecting ions into a drift region, said electrode being arranged to be energised in use in a substantially synchronised manner with the pulses of ions emerging from the exit of the fragmentation device.
50. A mass spectrometer as claimed in any preceding claim, wherein said fragmentation device is selected from the group consisting of: (i) an ion funnel comprising a plurality of electrodes having apertures therein through which ions are transmitted, wherein the diameter of said apertures becomes progressively smaller or larger (ii) an ion tunnel comprising a plurality of electrodes having apertures therein through which ions are transmitted, wherein the diameter of said apertures remains substantially constant; and (iii) a stack of plate, ring or wire loop electrodes.
51. A mass spectrometer as claimed in any preceding claim, wherein said fragmentation device comprises a plurality of electrodes, each electrode having an aperture through which ions are transmitted in use.
52. A mass spectrometer as claimed in any preceding claim, wherein each electrode has a substantially circular aperture.
53. A mass spectrometer as claimed in any preceding claim, wherein each electrode has a single aperture through which ions are transmitted in use.
54. A mass spectrometer as claimed in claim 51, 52 or 53, wherein the diameter of the apertures of at least 50%, 60%, 70%, 80%, 90% or 95% of the electrodes forming said fragmentation device is selected from the group consisting of: (i) less than or equal to 10 mm; (ii) less than or equal to 9 mm; (iii) less than or equal to 8 mm; (iv) less than or equal to 7 mm; (v) less than or equal to 6 mm; (vi) less than or equal to 5 mm; (vii) less than or equal to 4 mm; (viii) less than or equal to 3 mm; (ix) less than or equal to 2 mm; and (x) less than or equal to 1 mm.
55. A mass spectrometer as claimed in any preceding claim, wherein at least 50%, 60%, 70%, 80%, 90% or 95%
of the electrodes forming the fragmentation device have apertures which are substantially the same size or area.
of the electrodes forming the fragmentation device have apertures which are substantially the same size or area.
56. A mass spectrometer as claimed in any of claims 1-49, wherein said fragmentation device comprises a segmented rod set.
57. A mass spectrometer as claimed in any preceding claim, wherein said fragmentation device consists of:
(i) 10-20 electrodes; (ii) 20-30 electrodes; (iii) 30-40 electrodes; (iv) 40-50 electrodes; (v) 50-60 electrodes;
(vi) 60-70 electrodes; (vii) 70-80 electrodes; (viii) 80-90 electrodes; (ix) 90-100 electrodes; (x) 100-110 electrodes; (xi) 110-120 electrodes; (xii) 120-130 electrodes; (xiii) 130-140 electrodes; (xiv) 140-150 electrodes; or (xv) more than 150 electrodes.
(i) 10-20 electrodes; (ii) 20-30 electrodes; (iii) 30-40 electrodes; (iv) 40-50 electrodes; (v) 50-60 electrodes;
(vi) 60-70 electrodes; (vii) 70-80 electrodes; (viii) 80-90 electrodes; (ix) 90-100 electrodes; (x) 100-110 electrodes; (xi) 110-120 electrodes; (xii) 120-130 electrodes; (xiii) 130-140 electrodes; (xiv) 140-150 electrodes; or (xv) more than 150 electrodes.
58. A mass spectrometer as claimed in any preceding claim, wherein the thickness of at least 50%, 60%, 70%, 80%, 90% or 95% of said electrodes is selected from the group consisting of: (i) less than or equal to 3 mm;
(ii) less than or equal to 2.5 mm; (iii) less than or equal to 2.0 mm; (iv) less than or equal to 1.5 mm; (v) less than or equal to 1.0 mm: and (vi) less than or equal to 0.5 mm.
(ii) less than or equal to 2.5 mm; (iii) less than or equal to 2.0 mm; (iv) less than or equal to 1.5 mm; (v) less than or equal to 1.0 mm: and (vi) less than or equal to 0.5 mm.
59. A mass spectrometer as claimed in any preceding claim, wherein said fragmentation device has a length selected from the group consisting of: (i) less than 5 cm: (ii) 5-10 cm; (iii) 10-15 cm; (iv) 15-20 cm; (v) 20-25 cm; (vi) 25-30 cm; and (vii) greater than 30 cm.
60. A mass spectrometer as claimed in any preceding claim, wherein said fragmentation device comprises a housing having an upstream opening for allowing ions to enter said fragmentation device and a downstream opening for allowing ions to exit said fragmentation device.
61. A mass spectrometer as claimed in claim 60, wherein the fragmentation device further comprises an inlet port through which a collision gas is introduced.
62. A mass spectrometer as claimed in claim 61, wherein said collision gas comprises air and/or one or more inert gases and/or one or more non-inert gases.
63. A mass spectrometer as claimed in any preceding claim, wherein at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% of said electrodes are connected to both a DC and an AC or RF voltage supply.
64. A mass spectrometer as claimed in any preceding claim, wherein axially adjacent electrodes are supplied with AC or RF voltages having a phase difference of 180°.
65. A mass spectrometer as claimed in any preceding claim, further comprising an ion source selected from the group consisting of: (i) Electrospray ("ESI") ion source; (ii) Atmospheric Pressure Chemical Ionisation ("APCI") ion source; (iii) Atmospheric Pressure Photo Ionisation ("APPI") ion source; (iv) Matrix Assisted Laser Desorption Tonisation ("MALDI") ion source; (v) Laser Desorption Tonisation ("LDI") ion source; (vi) Inductively Coupled Plasma ("ICP") ion source; (vii) Electron Impact ("EI") ion source; (viii) Chemical Ionisation ("CI") ion source; (ix) a Fast Atom Bombardment ("FAB") ion source; and (x) a Liquid Secondary Ions Mass Spectrometry ("LSIMS") ion source.
66. A mass spectrometer as claimed in any of claims 1-64, further comprising a continuous ion source.
67. A mass spectrometer as claimed in any of claims 1-64, further comprising a pulsed ion source.
68. A mass spectrometer comprising:
a reaction cell wherein in use ions react and/or exchange charge with a gas in said reaction cell, said reaction cell comprising a plurality of electrodes wherein, in use, one or more transient DC voltages or one or more transient DC voltage waveforms are progressively applied to said electrodes so that ions are urged along said reaction cell.
a reaction cell wherein in use ions react and/or exchange charge with a gas in said reaction cell, said reaction cell comprising a plurality of electrodes wherein, in use, one or more transient DC voltages or one or more transient DC voltage waveforms are progressively applied to said electrodes so that ions are urged along said reaction cell.
69. A mass spectrometer comprising:
a cell comprising a gas for damping, collisionally cooling, decelerating, axially focusing or otherwise thermalizing ions without substantially fragmenting said ions, said cell comprising a plurality of electrodes wherein, in use, one or more transient DC voltages or-one or more transient DC voltage waveforms are progressively applied to said electrodes so that ions are urged along said cell.
a cell comprising a gas for damping, collisionally cooling, decelerating, axially focusing or otherwise thermalizing ions without substantially fragmenting said ions, said cell comprising a plurality of electrodes wherein, in use, one or more transient DC voltages or-one or more transient DC voltage waveforms are progressively applied to said electrodes so that ions are urged along said cell.
70. A mass spectrometer comprising:
an ion source;
a mass filter;
a fragmentation device comprising a plurality of electrodes wherein, in use, one or more transient DC
voltages or one or more transient DC voltage waveforms are progressively applied to said electrodes so that ions are urged along said fragmentation device; and a mass analyser.
an ion source;
a mass filter;
a fragmentation device comprising a plurality of electrodes wherein, in use, one or more transient DC
voltages or one or more transient DC voltage waveforms are progressively applied to said electrodes so that ions are urged along said fragmentation device; and a mass analyser.
71. A mass spectrometer as claimed in claim 70, further comprising an ion guide arranged upstream of said mass filter.
72. A mass spectrometer as claimed in claim 71, wherein said ion guide comprises a plurality of electrodes wherein at least some of said electrodes are connected to both a DC and an AC or RF voltage supply and wherein one or more transient DC voltages or one or more transient DC voltage waveforms are passed in use along at least a portion of the length of said ion guide to urge ions along said portion of the length of said ion guide.
73. A mass spectrometer as claimed in claim 70, 71 or 72, wherein said mass filter comprises a quadrupole mass filter.
74. A mass spectrometer as claimed in any of claims 70-73, wherein said mass analyser comprises a Time of Flight mass analyser, a quadrupole mass analyser, a Fourier Transform Ion Cyclotron Resonance ("FTICR") mass analyser, a 2D (linear) quadrupole ion trap or a 3D
(Paul) quadrupole ion trap.
(Paul) quadrupole ion trap.
75. A mass spectrometer comprising:
a fragmentation device comprising a plurality of electrodes having apertures, wherein ions are radially confined within said fragmentation device by an AC or RF
voltage such that adjacent electrodes have a phase difference of 180°, and wherein one or more DC voltage pulses or one or more transient DC voltage waveforms are applied successively to a plurality of said electrodes so that ions are urged towards an exit of said fragmentation device and have a transit time of less than 20 ms through said fragmentation device.
a fragmentation device comprising a plurality of electrodes having apertures, wherein ions are radially confined within said fragmentation device by an AC or RF
voltage such that adjacent electrodes have a phase difference of 180°, and wherein one or more DC voltage pulses or one or more transient DC voltage waveforms are applied successively to a plurality of said electrodes so that ions are urged towards an exit of said fragmentation device and have a transit time of less than 20 ms through said fragmentation device.
76. A mass spectrometer comprising a fragmentation device having a plurality of electrodes wherein one or more DC voltage pulses or one or more transient DC
voltage waveforms are applied to successive electrodes.
voltage waveforms are applied to successive electrodes.
77. R method of mass spectrometry comprising:
providing a fragmentation device comprising a plurality of electrodes; and progressively applying one or more transient DC
voltages or one or more transient DC voltage waveforms to said electrodes so that ions are fragmented within said fragmentation device and are urged along said fragmentation device.
providing a fragmentation device comprising a plurality of electrodes; and progressively applying one or more transient DC
voltages or one or more transient DC voltage waveforms to said electrodes so that ions are fragmented within said fragmentation device and are urged along said fragmentation device.
78. A method as claimed in claim 77, wherein said step of progressively applying one or more transient DC
voltages or one or more transient DC voltage waveforms comprises maintaining an axial voltage gradient which varies with time whilst ions are being transmitted through said fragmentation device.
voltages or one or more transient DC voltage waveforms comprises maintaining an axial voltage gradient which varies with time whilst ions are being transmitted through said fragmentation device.
79. A method as claimed in claim 77 or 78, wherein said one or more transient DC voltages or said one or more transient DC voltage waveforms are passed along said fragmentation device with a first velocity.
80. A method as claimed in claim 79, wherein said first velocity is selected from the group consisting of: (i) 10-250 m/s; (ii) 250-500 m/s; (iii) 500-750 m/s; (iv) 750-1000 m/s; (v) 1000-1250 m/s: (vi) 1250-1500 m/s;
(vii) 1500-1750 m/s; (viii) 1750-2000 m/s; (ix) 2000-2250 m/s; (x) 2250-2500 m/s; (xi) 2500-2750 m/s; (xii) 2750-3000 m/s; (xiii) 3000-3250 m/s; (xiv) 3250-3500 m/s; (xv) 3500-3750 m/s; (xvi) 3750-4000 m/s; (xvii) 4000-4250 m/s; (xviii) 4250-4500 m/s; (xix) 4500-4750 m/s; (xx) 4750-5000 m/s; and (xxi) > 5000 m/s.
(vii) 1500-1750 m/s; (viii) 1750-2000 m/s; (ix) 2000-2250 m/s; (x) 2250-2500 m/s; (xi) 2500-2750 m/s; (xii) 2750-3000 m/s; (xiii) 3000-3250 m/s; (xiv) 3250-3500 m/s; (xv) 3500-3750 m/s; (xvi) 3750-4000 m/s; (xvii) 4000-4250 m/s; (xviii) 4250-4500 m/s; (xix) 4500-4750 m/s; (xx) 4750-5000 m/s; and (xxi) > 5000 m/s.
81. A method of reacting ions and/or exchanging the charge of ions with a gas comprising:
providing a reaction cell comprising a plurality of electrodes; and progressively applying one or more transient DC
voltages or one or more transient DC voltage waveforms to said electrodes so that ions are urged along said reaction cell.
providing a reaction cell comprising a plurality of electrodes; and progressively applying one or more transient DC
voltages or one or more transient DC voltage waveforms to said electrodes so that ions are urged along said reaction cell.
82. A method of damping, collisionally cooling, decelerating, axially focusing or otherwise thermalizing ions without substantially fragmenting said ions comprising:
providing a cell comprising a plurality of electrodes; and progressively applying one or more transient DC
voltages to said electrodes so that ions are urged along said cell.
providing a cell comprising a plurality of electrodes; and progressively applying one or more transient DC
voltages to said electrodes so that ions are urged along said cell.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0212511A GB0212511D0 (en) | 2002-05-30 | 2002-05-30 | Mass spectrometer |
| GB0212511.0 | 2002-05-30 | ||
| GB0308346A GB0308346D0 (en) | 2002-05-30 | 2003-04-11 | Mass spectrometer |
| GB0308346.6 | 2003-04-11 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2430527A1 true CA2430527A1 (en) | 2003-11-30 |
| CA2430527C CA2430527C (en) | 2012-03-27 |
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| CA2430527A Expired - Lifetime CA2430527C (en) | 2002-05-30 | 2003-05-30 | Mass spectrometer |
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| AT (1) | ATE372587T1 (en) |
| CA (1) | CA2430527C (en) |
| DE (1) | DE60316070T2 (en) |
| GB (1) | GB2391698B (en) |
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| US20050242281A1 (en) * | 2004-04-30 | 2005-11-03 | Gangqiang Li | Unevenly segmented multipole |
| GB0426900D0 (en) | 2004-12-08 | 2005-01-12 | Micromass Ltd | Mass spectrometer |
| US7312442B2 (en) * | 2005-09-13 | 2007-12-25 | Agilent Technologies, Inc | Enhanced gradient multipole collision cell for higher duty cycle |
| DE102005044307B4 (en) | 2005-09-16 | 2008-04-17 | Bruker Daltonik Gmbh | Ionization of desorbed molecules |
| GB0723183D0 (en) * | 2007-11-23 | 2008-01-09 | Micromass Ltd | Mass spectrometer |
| US8269166B2 (en) * | 2009-02-05 | 2012-09-18 | Shimadzu Corporation | MS/MS mass spectrometer |
| GB201104665D0 (en) * | 2011-03-18 | 2011-05-04 | Shimadzu Res Lab Europe Ltd | Ion analysis apparatus and methods |
| GB201111568D0 (en) * | 2011-07-06 | 2011-08-24 | Micromass Ltd | Apparatus and method of mass spectrometry |
| GB201504817D0 (en) * | 2015-03-23 | 2015-05-06 | Micromass Ltd | Pre-filter fragmentation |
| US11600480B2 (en) | 2020-09-22 | 2023-03-07 | Thermo Finnigan Llc | Methods and apparatus for ion transfer by ion bunching |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5206506A (en) * | 1991-02-12 | 1993-04-27 | Kirchner Nicholas J | Ion processing: control and analysis |
| WO1994001883A1 (en) * | 1992-07-01 | 1994-01-20 | United States Department Of Energy | A method for discriminative particle separation |
| JPH11510946A (en) * | 1995-08-11 | 1999-09-21 | エムディーエス ヘルス グループ リミテッド | Spectrometer with axial electric field |
| GB2341270A (en) * | 1998-09-02 | 2000-03-08 | Shimadzu Corp | Mass spectrometer having ion lens composed of plurality of virtual rods comprising plurality of electrodes |
| CA2391148C (en) * | 2001-06-25 | 2008-02-19 | Micromass Limited | Mass spectrometer |
| CA2391140C (en) * | 2001-06-25 | 2008-10-07 | Micromass Limited | Mass spectrometer |
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2003
- 2003-05-30 AT AT03253411T patent/ATE372587T1/en not_active IP Right Cessation
- 2003-05-30 EP EP03253411A patent/EP1367632B1/en not_active Expired - Lifetime
- 2003-05-30 DE DE60316070T patent/DE60316070T2/en not_active Expired - Lifetime
- 2003-05-30 GB GB0312482A patent/GB2391698B/en not_active Expired - Lifetime
- 2003-05-30 CA CA2430527A patent/CA2430527C/en not_active Expired - Lifetime
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|---|---|
| GB2391698B (en) | 2004-07-21 |
| EP1367632B1 (en) | 2007-09-05 |
| GB2391698A (en) | 2004-02-11 |
| ATE372587T1 (en) | 2007-09-15 |
| CA2430527C (en) | 2012-03-27 |
| DE60316070D1 (en) | 2007-10-18 |
| DE60316070T2 (en) | 2008-06-05 |
| EP1367632A3 (en) | 2004-09-29 |
| GB0312482D0 (en) | 2003-07-09 |
| EP1367632A2 (en) | 2003-12-03 |
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