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CA2329122A1 - Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease - Google Patents

Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease Download PDF

Info

Publication number
CA2329122A1
CA2329122A1 CA002329122A CA2329122A CA2329122A1 CA 2329122 A1 CA2329122 A1 CA 2329122A1 CA 002329122 A CA002329122 A CA 002329122A CA 2329122 A CA2329122 A CA 2329122A CA 2329122 A1 CA2329122 A1 CA 2329122A1
Authority
CA
Canada
Prior art keywords
yes
exonuclease
nucleic acid
polynucleotide
treatment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002329122A
Other languages
English (en)
Inventor
Jay M. Short
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by Individual filed Critical Individual
Publication of CA2329122A1 publication Critical patent/CA2329122A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention a trait à des méthodes permettant d'obtenir de nouveaux polynucléotides ainsi que des polypeptides codés et ce, en faisant intervenir des techniques non stochastiques d'évolution dirigée (Direct Evolution<TM>). Ces méthodes de réassemblage d'acide nucléique induit par l'exonucléase présentent un avantage particulier, savoir la capacité à réassembler des brins nucléotidiques qui, autrement, poseraient des problèmes s'agissant de leur chimérisation. On peut utiliser ces méthodes de réassemblage d'acide nucléique induit par l'exonucléase, associées à d'autres méthodes de mutagenèse décrites dans cette invention. Ces méthodes reposent sur la mutagenèse à saturation de site polynucléotidique non stochastique (Gene Site Saturation Mutagenesis<TM>) ainsi que sur le réassemblage polynucléotidique non stochastique (Gene Reassembly<TM>). Cette invention porte également sur des méthodes d'obtention de nouvelles enzymes aux propriétés physiques et/ou biologiques optimisées. Il est possible, grâce à ces méthodes, de faire évoluer des vaccins génétiques, des enzymes, de petites molécules et d'autres molécules souhaitées vers des propriétés voulues. On peut par exemple obtenir des vecteurs de vaccins à efficacité accrue et les utiliser comme vaccins génétiques. Les vecteurs obtenus grâce à ces méthodes peuvent présenter, par exemple, une expression antigénique accrue, une assimilation accrue dans la cellule, une stabilité accrue dans la cellule, une capacité à adapter la réponse immunitaire, et analogue. Par ailleurs, cette invention concerne des procédés de préparation de diverses nouvelles molécules actives du point de vue biologique dans le domaine des antibiotiques, de la pharmacothérapie, et des caractères transgéniques.
CA002329122A 1999-03-26 2000-03-27 Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease Abandoned CA2329122A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US09/332,835 1999-06-14
US09/276,860 1999-06-14
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
PCT/US2000/008245 WO2000058517A1 (fr) 1999-03-26 2000-03-27 Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease

Publications (1)

Publication Number Publication Date
CA2329122A1 true CA2329122A1 (fr) 2000-10-05

Family

ID=26958167

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002329122A Abandoned CA2329122A1 (fr) 1999-03-26 2000-03-27 Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease

Country Status (6)

Country Link
EP (1) EP1092041A4 (fr)
JP (1) JP2004500019A (fr)
AU (1) AU4039400A (fr)
CA (1) CA2329122A1 (fr)
MX (1) MXPA00011569A (fr)
WO (1) WO2000058517A1 (fr)

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GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
EP1844144B1 (fr) * 2005-01-10 2010-04-14 MedImmune Limited Méthode de mutagenèse
CA2673954C (fr) 2007-01-25 2015-09-15 Danisco A/S Production d'une lipide acyltransferase a partir de cellules transformees de bacillus licheniformis
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
PL2139515T5 (pl) 2007-03-30 2024-04-08 The Research Foundation Of The State University Of New York Atenuowane wirusy przydatne w szczepionkach
KR20100086507A (ko) 2007-12-21 2010-07-30 대니스코 에이/에스 지질 아실트랜스퍼라제를 이용한 식용유 정제 방법
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JP2012527230A (ja) 2009-05-19 2012-11-08 デュポン ニュートリション バイオサイエンシーズ エーピーエス 使用
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
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Also Published As

Publication number Publication date
JP2004500019A (ja) 2004-01-08
WO2000058517A1 (fr) 2000-10-05
EP1092041A4 (fr) 2001-09-19
EP1092041A1 (fr) 2001-04-18
AU4039400A (en) 2000-10-16
MXPA00011569A (es) 2004-12-03

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Legal Events

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EEER Examination request
FZDE Discontinued