ATE260987T1 - Verfahren zum screening enzymatischer aktivität - Google Patents
Verfahren zum screening enzymatischer aktivitätInfo
- Publication number
- ATE260987T1 ATE260987T1 AT96942920T AT96942920T ATE260987T1 AT E260987 T1 ATE260987 T1 AT E260987T1 AT 96942920 T AT96942920 T AT 96942920T AT 96942920 T AT96942920 T AT 96942920T AT E260987 T1 ATE260987 T1 AT E260987T1
- Authority
- AT
- Austria
- Prior art keywords
- enzymatic activity
- dna
- screening
- activity
- screening enzymatic
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 230000002255 enzymatic effect Effects 0.000 title abstract 3
- 238000012216 screening Methods 0.000 title abstract 2
- 230000000694 effects Effects 0.000 abstract 1
- 238000002703 mutagenesis Methods 0.000 abstract 1
- 231100000350 mutagenesis Toxicity 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
- C12Y301/03001—Alkaline phosphatase (3.1.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Ecology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US831195P | 1995-12-07 | 1995-12-07 | |
| US831795P | 1995-12-07 | 1995-12-07 | |
| US831695P | 1995-12-07 | 1995-12-07 | |
| US08/651,568 US5939250A (en) | 1995-12-07 | 1996-05-22 | Production of enzymes having desired activities by mutagenesis |
| US08/692,002 US6054267A (en) | 1995-12-07 | 1996-08-02 | Method for screening for enzyme activity |
| PCT/US1996/019457 WO1997020918A1 (en) | 1995-12-07 | 1996-12-06 | Method of screening for enzyme activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE260987T1 true ATE260987T1 (de) | 2004-03-15 |
Family
ID=27533271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96942920T ATE260987T1 (de) | 1995-12-07 | 1996-12-06 | Verfahren zum screening enzymatischer aktivität |
Country Status (8)
| Country | Link |
|---|---|
| EP (2) | EP1130090A3 (de) |
| JP (2) | JP2000501606A (de) |
| AT (1) | ATE260987T1 (de) |
| AU (1) | AU720334B2 (de) |
| CA (1) | CA2239686A1 (de) |
| DE (1) | DE69631787T2 (de) |
| IL (1) | IL124794A (de) |
| WO (1) | WO1997020918A1 (de) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6004788A (en) | 1995-07-18 | 1999-12-21 | Diversa Corporation | Enzyme kits and libraries |
| US20030215798A1 (en) * | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
| AU726017C (en) * | 1996-01-11 | 2006-09-14 | Bp Corporation North America Inc. | Glycosidase enzymes |
| DE69738631T2 (de) * | 1996-01-11 | 2009-05-14 | Verenium Corp., San Diego | Endoglukanase von Thermotoga |
| US6242211B1 (en) | 1996-04-24 | 2001-06-05 | Terragen Discovery, Inc. | Methods for generating and screening novel metabolic pathways |
| US5789228A (en) | 1996-05-22 | 1998-08-04 | Diversa Corporation | Endoglucanases |
| US7465571B1 (en) | 1996-05-22 | 2008-12-16 | Verenium Corporation | Endoglucanases |
| PT1027367E (pt) | 1996-12-06 | 2005-09-30 | Diversa Corp | Enzimas de glicosidade |
| US7279316B2 (en) | 1996-12-06 | 2007-10-09 | Verenium Corporation | Enzymes having glycosidase activity and methods of use thereof |
| US6268133B1 (en) * | 1997-06-25 | 2001-07-31 | Invitrogen Corporation | Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence |
| DE19731990A1 (de) * | 1997-07-25 | 1999-01-28 | Studiengesellschaft Kohle Mbh | Verfahren zur Herstellung und Identifizierung von neuen Hydrolasen mit verbesserten Eigenschaften |
| US6261842B1 (en) | 1997-10-23 | 2001-07-17 | Wisconsin Alumni Research Foundation | Microorganism genomics, compositions and methods related thereto |
| AU4039400A (en) * | 1999-03-26 | 2000-10-16 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| AU2001234980A1 (en) | 2000-02-09 | 2001-08-20 | Genvec, Inc. | Methods of preparing and using a viral vector library |
| WO2002004628A2 (en) | 2000-07-06 | 2002-01-17 | Genvec, Inc. | Method of identifying a binding partner of a gene product |
| JP4114075B2 (ja) * | 2004-02-16 | 2008-07-09 | ヒューマン・メタボローム・テクノロジーズ株式会社 | 遺伝子産物の機能同定方法及び結合物質同定方法 |
| US20050221339A1 (en) * | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
| JP4568571B2 (ja) * | 2004-10-06 | 2010-10-27 | ユニバーサル・バイオ・リサーチ株式会社 | 熱安定性又は熱活性ｄｎａポリメラーゼ及びそれをコードするｄｎａ |
| EP2406399B1 (de) | 2009-03-09 | 2018-02-14 | Bioatla, LLC | Mirac-proteine |
| ES2741175T3 (es) * | 2010-07-16 | 2020-02-10 | Bioatla Llc | Nuevos métodos de evolución de proteínas |
| CN109508300B (zh) * | 2018-11-22 | 2022-07-05 | 广州爱云信息技术有限公司 | 一种磁盘碎片整理方法、设备及计算机可读存储介质 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0463707A1 (de) * | 1985-12-17 | 1992-01-02 | Lubrizol Genetics Inc. | Herstellung von Polyketid-Antibiotika |
| EP0441252B1 (de) * | 1990-02-07 | 1997-09-03 | Abbott Laboratories | Alkalische Phosphatase mit verbesserter spezifischer Aktivität zur Verwendung in Indikatorreagenzien |
| US5352778A (en) * | 1990-04-26 | 1994-10-04 | New England Biolabs, Inc. | Recombinant thermostable DNA polymerase from archaebacteria |
| WO1992005249A1 (en) * | 1990-09-13 | 1992-04-02 | Novo Nordisk A/S | Lipase variants |
| DE547920T1 (de) * | 1991-12-18 | 1994-02-03 | New England Biolabs Inc | Rekombinante thermostabile DNA-Polymerase von Archaebakterie. |
| US5958672A (en) * | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
-
1996
- 1996-12-06 AT AT96942920T patent/ATE260987T1/de active
- 1996-12-06 DE DE69631787T patent/DE69631787T2/de not_active Expired - Lifetime
- 1996-12-06 CA CA002239686A patent/CA2239686A1/en not_active Abandoned
- 1996-12-06 AU AU11489/97A patent/AU720334B2/en not_active Expired
- 1996-12-06 EP EP01102857A patent/EP1130090A3/de not_active Ceased
- 1996-12-06 JP JP9521457A patent/JP2000501606A/ja active Pending
- 1996-12-06 IL IL124794A patent/IL124794A/en not_active IP Right Cessation
- 1996-12-06 WO PCT/US1996/019457 patent/WO1997020918A1/en not_active Ceased
- 1996-12-06 EP EP96942920A patent/EP0866853B1/de not_active Expired - Lifetime
-
2000
- 2000-08-08 JP JP2000239967A patent/JP2001078786A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO1997020918A1 (en) | 1997-06-12 |
| EP1130090A2 (de) | 2001-09-05 |
| DE69631787D1 (de) | 2004-04-08 |
| EP0866853A1 (de) | 1998-09-30 |
| DE69631787T2 (de) | 2005-03-10 |
| CA2239686A1 (en) | 1997-06-12 |
| AU1148997A (en) | 1997-06-27 |
| EP1130090A3 (de) | 2003-11-26 |
| EP0866853A4 (de) | 1999-09-22 |
| AU720334B2 (en) | 2000-05-25 |
| JP2001078786A (ja) | 2001-03-27 |
| IL124794A (en) | 2008-06-05 |
| EP0866853B1 (de) | 2004-03-03 |
| JP2000501606A (ja) | 2000-02-15 |
| IL124794A0 (en) | 1999-01-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69631787D1 (de) | Verfahren zum screening enzymatischer aktivität | |
| DE3481775D1 (de) | Verfahren zum raschen nachweis von mikroorganismen in klinischen proben. | |
| ATE432342T1 (de) | Eine methode zum entwurf von alpha-amylase mutanten mit vorbestimmten eigenschaften | |
| DE69430054D1 (de) | Verfahren zum einbetten von tierkulturzellen | |
| ATE3993T1 (de) | Mittel zum nachweis esterolytischer und/oder proteolytischer enzyme und dafuer als substrate geeignete phenoxy-aminosaeure- und phenoxypeptidester, verfahren zu deren herstellung sowie deren verwendung zur herstellung der erfindungsgemaessen mittel. | |
| DE69637683D1 (de) | Verfahren zum Einführen von Nukleinsäuren in Zellen für therapeutische und diagnostische Verwendungen | |
| DE3575621D1 (de) | Verfahren und anordnung zum kennzeichnen und erkennen von gegenstaenden. | |
| DE69731222D1 (de) | Verfahren zum entwerfen von reifen, optimierungsanalysereinrichtung und speichermedium zur aufnahme des optimierungsanalyseprogramms | |
| ATE220657T1 (de) | Verfahren zum herstellen mit grosser ausbeute von methylmethacrylat oder methacrylsäure | |
| DE69934035D1 (de) | Vorrichtung zum Aktualisieren von einem Kennwort und dafür verwendetes Aufzeichnungsmedium | |
| DE3761706D1 (de) | Verfahren und vorrichtung zum nachverbrennen von prozess-abgas. | |
| DE69610887D1 (de) | Verfahren und Vorrichtung zum Kleben von Platten | |
| DE3575742D1 (de) | Verfahren und anlage zum durchfuehren eines mikrobiologischen oder enzymatischen verfahrens. | |
| DE68915439D1 (de) | Verfahren und vorrichtung zum sammeln und nachweisen von mikroorganismen. | |
| DE69518378D1 (de) | Verfahren zum Konservieren von ein Suspension von Zellen oder immobilisierten Zellen | |
| DE69031782D1 (de) | Verfahren zum Ersetzen von Speichermodulen in einem Computersystem und Computersystem zur Durchführung des Verfahrens | |
| DE69534698D1 (de) | Verfahren und Vorrichtung zum Vermittlen von Zellen | |
| DE69621008D1 (de) | Verfahren zum zerkleinern von vorzerkleinertem erz | |
| DE59303165D1 (de) | Dunstabzugshaube und Verfahren zum Absaugen von Staub oder Dunst | |
| ATE312434T1 (de) | Verfahren, vorrichtung und träger zum aufnehmen von multiwort-information | |
| DE69637478D1 (de) | Verfahren und anordnung zum erzeugen von mehreren quadraturmodulierten trägern | |
| DE3672920D1 (de) | Verfahren zur herstellung immobilisierter enzyme und vorrichtung zur durchfuehrung des verfahrens. | |
| DE69509104D1 (de) | Testsatz und verfahren zum nachweiss zielzellen und moleküle | |
| DE59703769D1 (de) | Verfahren und eine Vorrichtung zum elektrolytischen Beizen von metallischen Bändern | |
| DE60124780D1 (de) | Vorrichtung und verfahren zur serienvermehrung von säugerzellen zum animpfen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 0866853 Country of ref document: EP |
|
| EEFA | Change of the company name |