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BRPI0708477B8 - Método para identificar uma primeira planta de milho ou um primeiro germoplasma de milho compreendendo em seu genoma um gene de diaglicerol o-aciltransferase do tipo 1 (dgat), polinucleotídeo isolado, método para produzir uma planta ou parte de planta possuindo um fenótipo selecionado, método para identificar a presença de um lócus marcador - Google Patents

Método para identificar uma primeira planta de milho ou um primeiro germoplasma de milho compreendendo em seu genoma um gene de diaglicerol o-aciltransferase do tipo 1 (dgat), polinucleotídeo isolado, método para produzir uma planta ou parte de planta possuindo um fenótipo selecionado, método para identificar a presença de um lócus marcador

Info

Publication number
BRPI0708477B8
BRPI0708477B8 BRPI0708477A BRPI0708477A BRPI0708477B8 BR PI0708477 B8 BRPI0708477 B8 BR PI0708477B8 BR PI0708477 A BRPI0708477 A BR PI0708477A BR PI0708477 A BRPI0708477 A BR PI0708477A BR PI0708477 B8 BRPI0708477 B8 BR PI0708477B8
Authority
BR
Brazil
Prior art keywords
plant
oleic acid
marker
corn
increased
Prior art date
Application number
BRPI0708477A
Other languages
English (en)
Inventor
b allen William
Shen Bo
C Tarczynski Mitchell
E Williams Mark
Zheng Peizhong
Zhong Gan-Yuan
Original Assignee
Pioneer Hi Bred Int
Du Pont
Corteva Agriscience Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int, Du Pont, Corteva Agriscience Llc filed Critical Pioneer Hi Bred Int
Publication of BRPI0708477A2 publication Critical patent/BRPI0708477A2/pt
Publication of BRPI0708477B1 publication Critical patent/BRPI0708477B1/pt
Publication of BRPI0708477B8 publication Critical patent/BRPI0708477B8/pt

Links

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/104165—Lipid, cholesterol, or triglyceride standard or control

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

COMPOSIÇÕES RELACIONADAS AO LÓCUS 6 DE TRAÇO QUANTITATIVO (QTL6) EM MILHO E METODOS DE USO Composições relacionadas ao lócus 6 de traço quantitativo (QTL6) em milho e métodos para seu uso são fornecidos. As composições são loci marcadores moleculares novos que são geneticameflte ligados ao QTL6 e que estão associados ao conteúdo de óleo aumentado e/ou conteúdo de ácido oléico aumentado e/ou relação entre ácido oléico/ácido linoleico aumentada de uma planta ou parte de planta. Estes novos marcadores são caracterizados pela presença de pelo menos um polimorfismo relativo ao lôcus marcador correspondente da região de QTL6 de plantas de milho com conteúdo de óleo baixo, conteúdo de ácido oléico baixo. Em algumas modalidades, o novo loci marcadorcompreende a seqúência codificadora para um polipeptídeo DGAT 1-2 de milho ou variante biologicamente ativa deste. O loci marcador da invenção, e os fragmentos adequados deste,são úteis nos métodos da invenção para manipular o conteúdo de óleo e/ou ácido oléico e/ou a relação entre ácido oléico e ácido linoleico de uma planta ou parte de planta, para a seleção assistida por marcador de uma planta, por exemplo, uma planta de milho, ou parte da planta, possuindo um conteúdo de óleo aumentado e/ou conteúdo de ácido oléico aumentado e/ou uma relação entre ácido oléico e ácido linoleico aumentada, e para a reprodução assistida por marcador do traço de alto conteúdo de óleo e/ou alto conteúdo de ácido oléico.
BRPI0708477A 2006-03-01 2007-03-01 Método para identificar uma primeira planta de milho ou um primeiro germoplasma de milho compreendendo em seu genoma um gene de diaglicerol o-aciltransferase do tipo 1 (dgat), polinucleotídeo isolado, método para produzir uma planta ou parte de planta possuindo um fenótipo selecionado, método para identificar a presença de um lócus marcador BRPI0708477B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US77817806P 2006-03-01 2006-03-01
US60/778,178 2006-03-01
PCT/US2007/063088 WO2007103738A2 (en) 2006-03-01 2007-03-01 Compositions related to the quantitative trait locus 6 (qtl6) in maize and methods of use

Publications (3)

Publication Number Publication Date
BRPI0708477A2 BRPI0708477A2 (pt) 2011-05-31
BRPI0708477B1 BRPI0708477B1 (pt) 2017-07-11
BRPI0708477B8 true BRPI0708477B8 (pt) 2022-12-06

Family

ID=38475689

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0708477A BRPI0708477B8 (pt) 2006-03-01 2007-03-01 Método para identificar uma primeira planta de milho ou um primeiro germoplasma de milho compreendendo em seu genoma um gene de diaglicerol o-aciltransferase do tipo 1 (dgat), polinucleotídeo isolado, método para produzir uma planta ou parte de planta possuindo um fenótipo selecionado, método para identificar a presença de um lócus marcador

Country Status (7)

Country Link
US (3) US7981677B2 (pt)
EP (1) EP1991047B1 (pt)
AU (1) AU2007223426B2 (pt)
BR (1) BRPI0708477B8 (pt)
CA (1) CA2638739A1 (pt)
MX (1) MX2008010989A (pt)
WO (1) WO2007103738A2 (pt)

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JP2007524394A (ja) 2003-06-27 2007-08-30 モンサント テクノロジー エルエルシー 植物中の油レベルの上昇
WO2005063988A1 (en) 2003-12-23 2005-07-14 Pioneer Hi-Bred International, Inc. Alteration of oil traits in plants
MX2008010992A (es) * 2006-03-01 2008-11-27 Pioneer Hi Bred Int Composiciones relacionadas con el locus 6 de rasgo cuantitativo (qtl6) en maiz y metodos de uso.
EP1991047B1 (en) * 2006-03-01 2012-09-26 Pioneer Hi-Bred International Inc. Compositions related to the quantitative trait locus 6 (qtl6) in maize and methods of use

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US7981677B2 (en) 2011-07-19
US8319010B2 (en) 2012-11-27
CA2638739A1 (en) 2007-09-13
WO2007103738A3 (en) 2007-12-27
AU2007223426A1 (en) 2007-09-13
US9137957B2 (en) 2015-09-22
US20130198890A1 (en) 2013-08-01
EP1991047A2 (en) 2008-11-19
BRPI0708477A2 (pt) 2011-05-31
BRPI0708477B1 (pt) 2017-07-11
EP1991047B1 (en) 2012-09-26
AU2007223426B2 (en) 2013-05-09
US20110252499A1 (en) 2011-10-13
WO2007103738A8 (en) 2009-07-30
MX2008010989A (es) 2009-01-26
US20070294781A1 (en) 2007-12-20

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