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AR065284A1 - Composiciones y metodos que utilizan arn de interferencia de tipo cdpk para el control de nematodos - Google Patents

Composiciones y metodos que utilizan arn de interferencia de tipo cdpk para el control de nematodos

Info

Publication number
AR065284A1
AR065284A1 ARP080100568A ARP080100568A AR065284A1 AR 065284 A1 AR065284 A1 AR 065284A1 AR P080100568 A ARP080100568 A AR P080100568A AR P080100568 A ARP080100568 A AR P080100568A AR 065284 A1 AR065284 A1 AR 065284A1
Authority
AR
Argentina
Prior art keywords
polynucleotide
sequence
consecutive nucleotides
seq
indicated
Prior art date
Application number
ARP080100568A
Other languages
English (en)
Original Assignee
Basf Plant Science Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh filed Critical Basf Plant Science Gmbh
Publication of AR065284A1 publication Critical patent/AR065284A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)

Abstract

Composiciones de ARN bicatenario y plantas transgénicas capaces de inhibir la expresion de genes esenciales para establecer o mantener en una planta la infestacion por nemátodos, y con métodos asociados con aquellas. Específicamente se relaciona con el uso de ARN de interferencia para inhibir la expresion de un gen vegetal blanco, que es un gen (le tipo CDPK, y se relaciona con a generacion de plantas que tienen mayor resistencia a nemátodos parasitarios. Reivindicacion 1: Una molécula de dsARN caracterizada porque comprende i) una primera cadena que comprende una secuencia sustancialmente idéntica a una porcion de un gen de tipo CDPK, y ii) una segunda cadena que comprende una secuencia sustancialmente complementaria de la primera cadena, en donde la porcion del gen de tipo CDPK es de un polinucleotido seleccionado del grupo que consiste en: a) un polinucleotido que comprende una secuencia como se indica en SEQ ID N°:1, 2, 4, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24 o 26; b) un polinucleotido que codifica un polipéptido que tiene una secuencia como se indica en SEQ ID N°:3, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25 o 27; c) un polinucleotido que tiene 70% de identidad de secuencia con un polinucleotido que tiene una secuencia como se indica en SEQ ID N°: 1, 2, 4, 5, 8, 10 12, 14, 16, 18, 20, 22, 24 o 26; 9; d) un polinucleotido que codifica un polipéptido que tiene 70% de identidad de secuencia con un polipéptido que tiene una secuencia como se indica en SEQ ID N°: 3, 7,9, 11, 13, 15, 17, 19, 21, 23, 25 o 27; e) un polinucleotido que comprende un fragmento de al menos 19 nucleotidos consecutivos, o al menos 50 nucleotidos consecutivos, o al menos 100 nucleotidos consecutivos, o al menos 200 nucleotidos consecutivos de un polinucleotido que tiene una secuencia como se indica en SEQ ID N°: 1, 2, 4, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24 o 26; f) un polinucleotido que comprende un fragmento que codifica una porcion biologicamente activa de un polipéptido que tiene una secuencia como se indica en SEQ ID N°: 3, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25 o 27; g) un polinucleotido que se hibridiza en condiciones rigurosas a un polinucleotido que comprende al menos 19 nucleotidos consecutivos, o al menos 50 nucleotidos consecutivos, o al menos 100 nucleotidos consecutivos, o al menos 200 nucleotidos consecutivos de un polinucleotido que tiene una secuencia como se indica en SEQ ID N°: 1, 2, 4, 5, 8, 10 12, 14, 16, 18, 20, 22, 24 o 26; y h) un polinucleotido que se hibridiza en condiciones rigurosas a un polinucleotido que comprende al menos 19 nucleotidos consecutivos, o al menos 50 nucleotidos consecutivos, o al menos 100 nucleotidos consecutivos, o al menos que tiene una secuencie como se indica en SEQ ID N°: 3, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25 o 27.
ARP080100568A 2007-02-09 2008-02-08 Composiciones y metodos que utilizan arn de interferencia de tipo cdpk para el control de nematodos AR065284A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US90046607P 2007-02-09 2007-02-09

Publications (1)

Publication Number Publication Date
AR065284A1 true AR065284A1 (es) 2009-05-27

Family

ID=39306022

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP080100568A AR065284A1 (es) 2007-02-09 2008-02-08 Composiciones y metodos que utilizan arn de interferencia de tipo cdpk para el control de nematodos

Country Status (10)

Country Link
US (1) US20100017910A1 (es)
EP (1) EP2115148B1 (es)
CN (1) CN101631867A (es)
AR (1) AR065284A1 (es)
AT (1) ATE524553T1 (es)
BR (1) BRPI0807204A2 (es)
CA (1) CA2677630A1 (es)
ES (1) ES2373614T3 (es)
MX (1) MX2009007766A (es)
WO (1) WO2008095970A1 (es)

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CN101631454A (zh) 2006-12-08 2010-01-20 衣阿华州立大学研究基金公司 参与硝酸盐吸收和代谢的植物基因
EA022553B1 (ru) 2010-01-22 2016-01-29 Байер Интеллектуэль Проперти Гмбх Применение комбинации биологически активных веществ, набор и средство, содержащие комбинацию биологически активных веществ, для борьбы с вредителями животного происхождения и способ улучшения использования продукционного потенциала трансгенного растения
WO2011104153A1 (en) * 2010-02-23 2011-09-01 Basf Plant Science Company Gmbh Nematode-resistant transgenic plants
EP2460406A1 (en) 2010-12-01 2012-06-06 Bayer CropScience AG Use of fluopyram for controlling nematodes in nematode resistant crops
RU2610088C2 (ru) 2010-09-22 2017-02-07 Байер Интеллектуэль Проперти Гмбх Применение активных ингредиентов для борьбы с нематодами у сельскохозяйственных культур, резистентных к нематодам
BR112014002855A2 (pt) 2011-08-10 2017-02-21 Bayer Ip Gmbh combinações do composto ativo que incluem derivados específicos do ácido tetrâmico
IN2014CN04325A (es) 2011-12-19 2015-09-04 Bayer Cropscience Ag
EP2622961A1 (en) 2012-02-02 2013-08-07 Bayer CropScience AG Acive compound combinations
TWI654180B (zh) 2012-06-29 2019-03-21 美商艾佛艾姆希公司 殺真菌之雜環羧醯胺
WO2014090765A1 (en) 2012-12-12 2014-06-19 Bayer Cropscience Ag Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops
BR112018002567B1 (pt) 2015-08-07 2023-10-31 BASF Agricultural Solutions Seed US LLC Gene recombinante, célula hospedeira, método de produção de uma planta transgênica, método de efetuação de expressão preferencial em raiz de um ácido nucleico, método para alterar a tolerância ao estresse biótico ou abiótico, arquitetura de raiz, eficiência no uso de nutriente, ou rendimento de uma planta e uso de um ácido nucleico isolado
CN113046375B (zh) * 2021-05-19 2023-06-27 新疆农业科学院园艺作物研究所 SpCPK33基因及其编码蛋白在调控番茄耐旱性中的应用

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Also Published As

Publication number Publication date
EP2115148A1 (en) 2009-11-11
ES2373614T3 (es) 2012-02-07
EP2115148B1 (en) 2011-09-14
CA2677630A1 (en) 2008-08-14
CN101631867A (zh) 2010-01-20
WO2008095970A1 (en) 2008-08-14
MX2009007766A (es) 2009-07-30
BRPI0807204A2 (pt) 2014-06-03
ATE524553T1 (de) 2011-09-15
US20100017910A1 (en) 2010-01-21

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